DESC:FIELD OF THE INVENTION
[0001] The present disclosure relates generally to the field of cleaning formulation. Particularly, the present disclosure relates to a floor cleaning concentrate formulation and method of preparation thereof.

BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] A wide variety of floor cleaning compositions, particularly, floor cleaning compositions, which are specifically formulated to address the particular technical requirements of different flooring materials (including marble, tile, linoleum etc.), or to address the specific technical problems associated with the treatment and/or removal of particular classes of soils from particular flooring materials are known. A great number of such known floor cleaning products include a substantial amount of one or more organic compounds, particularly traditional soap constituents, in a large amount which have been found to cause the deposition and/or formation of undesired surface deposits such as surface streaks or sticky film.
[0004] A further shortcoming of many known compositions available in the market is lack of either the disinfectant action or the cleansing action i.e. the conventional compositions primarily composed of only surfactant are unable to provide effective disinfection, whereas others primarily composed of harsh acidic or basic solutions tend to damage the surface. Such floor cleaning products containing volatile organic materials may damage the finish of the flooring surface; further volatile organic materials are also becoming increasingly undesirable owing to environmental and user/worker safety concerns.
[0005] There is therefore an unmet need in the art to develop a new and improved floor cleaning formulation that is safe, effective and economical, while exhibiting requisite anti-microbial effect. The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art.are found with most all of these therapies.

OBJECTS OF THE INVENTION
[0006] Primary object of the present invention is to provide a floor cleaning formulation that may overcome one or more limitations associated with the conventional formulations.
[0007] It is an object of the present disclosure to provide a floor cleaning formulation that exhibits excellent cleansing and antimicrobial properties.
[0008] It is an object of the present disclosure to provide a formulation that exhibits excellent solubility, foam stability and disinfectant activity.
[0009] It is an object of the present disclosure to provide a formulation that exhibits synergistic activity and/or functional reciprocity.
[00010] It is an object of the present disclosure to provide a formulation that is safe in use.
[00011] It is an object of the present disclosure to provide a formulation that is easy to prepare and is economical.
[00012] Another object of the present disclosure is to provide a method of preparation of the floor cleaning concentrate formulation.

SUMMARY OF THE INVENTION
[00013] The present disclosure relates generally to the field of cleaning formulations. Particularly, the present disclosure relates to a floor cleaning concentrate formulation and method of preparation thereof.
[00014] An aspect of the present disclosure relates to a floor cleaning concentrate formulation, said formulation including: (a) a cleansing and antimicrobial agent in an amount ranging from 50 to 80 % by weight of the formulation, said agent including a combination of benzalkonium chloride (BKC), hydrogen peroxide and povidone iodine; and (b) the remainder being an auxiliary agent.
[00015] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation, hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation and povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation.
[00016] In an embodiment, the auxiliary agent includes: (i) a foam stabilizer selected from cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), caprylyl, coco glucoside, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroylsarcosinate, sodium pareth sulfate, sodium stearate alpha olefin sulfonate and combination thereof; (ii) an auxiliary antimicrobial agent selected from tulsi extract, neem extract, tea tree oil, peppermint oil, cinnamon oil, thyme oil, lemon extract, vinegar, eucalyptus oil, clove oil, lemon, lavender, oregano and combinations thereof; (iii) a preservative selected from tetrasodium EDTA, citric acid and combinations thereof; and (iv) optionally, a fragrance and a coloring agent.
[00017] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation; hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation; povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 5 to 15 % by weight of the formulation; citric acid in an amount ranging from 5 to 15 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 1 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 1 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 1 % by weight of the formulation; a fragrance in an amount ranging from 1 to 20 % by weight of the formulation; and a coloring agent in an amount ranging from 0.2 to 3 % by weight of the formulation.
[00018] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 45 % by weight of the formulation; hydrogen peroxide in an amount ranging from 17 to 27 % by weight of the formulation; povidone iodine in an amount ranging from 3 to 7 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 7 to 12 % by weight of the formulation; citric acid in an amount ranging from 7 to 12 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 0.5 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; a fragrance in an amount ranging from 5 to 15 % by weight of the formulation and a coloring agent in an amount ranging from 0.2 to 2 % by weight of the formulation.
[00019] Another aspect of the present disclosure relates to a method of preparing a floor cleaning concentrate formulation, said method including the steps of: preparing a first pre-mix by uniformly mixing benzalkonium chloride (BKC), hydrogen peroxide and foam stabilizer; preparing a second pre-mix by grinding povidone iodine, an auxiliary antimicrobial agent, preservative and optionally, a coloring agent and a fragrance; and mixing the first pre-mix and the second pre-mix to prepare the formulation. In an embodiment, the foam stabilizer selected from cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), caprylyl, coco glucoside, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroylsarcosinate, sodium pareth sulfate, sodium stearate, alpha olefin sulfonate and combination thereof. In an embodiment, the auxiliary antimicrobial agent selected from Tulsi Extract, Neem Extract, tea tree oil, peppermint oil, cinnamon oil, thyme oil, lemon extract, vinegar, eucalyptus oil, clove oil, lemon, lavender, oregano and combinations thereof. In an embodiment, the preservative is selected from tetrasodium EDTA, citric acid and combinations thereof.
[00020] Other aspects, advantages, and salient features of the invention will become apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the exemplary embodiments of the invention.

DETAILED DESCRIPTION OF THE INVENTION
[00021] The embodiments herein and the various features and advantageous details thereof are explained more comprehensively with reference to the non-limiting embodiments that are detailed in the following description. Descriptions of well-known components and processing techniques are omitted so as to not unnecessarily obscure the embodiments herein. The examples used herein are intended merely to facilitate an understanding of the ways in which the embodiments herein may be practiced and to further enable those of skill in the art to practice the embodiments herein. Accordingly, the examples should not be construed as limiting the scope of the embodiments herein.
[00022] Unless otherwise specified, all terms used in disclosing the invention, including technical and scientific terms, have the meaning as commonly understood by one of ordinary skills in the art to which this invention belongs. By means of further guidance, term definitions may be included to better appreciate the teaching of the present invention.
[00023] As used in the description herein, the meaning of “a,” “an,” and “the” includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of “in” includes “in” and “on” unless the context clearly dictates otherwise.
[00024] As used herein, the terms “comprise”, “comprises”, “comprising”, “include”, “includes”, and “including” are meant to be non- limiting, i.e., other steps and other ingredients which do not affect the end of result can be added. The above terms encompass the terms “consisting of” and “consisting essentially of”.
[00025] As used herein, the terms “formulation”, “blend”, or “mixture” are all intended to be used interchangeably.
[00026] The terms “weight percent”, “vol-%”, “percent by weight”, “% by weight”, and variations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the formulation and multiplied by 100. It is understood that, as used here, “percent”, “%”, and the like are intended to be synonymous with “weight percent”, “vol-%”, etc.
[00027] The terms “hard surface cleaner”, and variations thereof, as used herein, refer to the hard-surfaces typically found in and around residential dwellings like bathrooms, kitchens, basements and garages, e.g., floors, walls, tiles, windows, sinks, showers, shower plasticized curtains, wash basins, drains, dishes, fixtures, and fittings and the like made of different materials like fiberglass and other car materials, leather, ceramic, painted and un-painted wood or concrete, varnished or sealed, plaster, bricks, vinyl, no-wax vinyl, linoleum, marble, melamine, glass, any plastics, metals, chromed surfaces, washing machines, automatic dryers, refrigerators, freezers, ovens, microwave ovens, dishwashers , medical facilities, e.g., hospitals, clinics as well as laboratories among other industrial and/or commercial settings including, but not limited to restaurants, full service and fast food, sports facilities and other facilities using janitorial staff, and those found in cleaning industrial parts such as automobile and airplane engines, metal parts, in removal of oils, greases and lubricants, attached thereto, on, in or and the like.
[00028] The terms “kitchen cleaner” and variations thereof, as used herein, refer to the kitchen surfaces including but not limited to stove, oven, pots, pans, and the like.
[00029] In some embodiments, the numbers expressing quantities of ingredients, properties such as concentration, reaction conditions, and so forth, used to describe and claim certain embodiments of the invention are to be understood as being modified in some instances by the term “about”. Accordingly, in some embodiments, the numerical parameters set forth in the written description are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable.
[00030] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.
[00031] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[00032] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.
[00033] The present disclosure relates generally to the field of cleaning formulation. Particularly, the present disclosure relates to a floor cleaning concentrate formulation and method of preparation thereof.
[00034] An aspect of the present disclosure relates to a floor cleaning concentrate formulation, said formulation including: (a) a cleansing agent; and an antimicrobial agent in an amount ranging from 50 to 80 % by weight of the formulation, said agent including a combination of benzalkonium chloride (BKC), hydrogen peroxide and povidone iodine; and (b) the remainder being an auxiliary agent.
[00035] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation, hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation and povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation.
[00036] In an embodiment, the auxiliary agent includes: (i) a foam stabilizer selected from cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), caprylyl, coco glucoside, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroylsarcosinate, sodium pareth sulfate, sodium stearate alpha olefin sulfonate and combination thereof; (ii) an auxiliary antimicrobial agent selected from tulsi extract, neem extract, tea tree oil, peppermint oil, cinnamon oil, thyme oil, lemon extract, vinegar, eucalyptus oil, clove oil, lemon, lavender, oregano and combinations thereof; (iii) a preservative selected from tetrasodium EDTA, citric acid and combinations thereof; and (iv) optionally, a fragrance and a coloring agent.
[00037] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation; hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation; povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 5 to 15 % by weight of the formulation; citric acid in an amount ranging from 5 to 15 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 1 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 1 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 1 % by weight of the formulation; a fragrance in an amount ranging from 1 to 20 % by weight of the formulation; and a coloring agent in an amount ranging from 0.2 to 3 % by weight of the formulation.
[00038] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 45 % by weight of the formulation; hydrogen peroxide in an amount ranging from 17 to 27 % by weight of the formulation; povidone iodine in an amount ranging from 3 to 7 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 7 to 12 % by weight of the formulation; citric acid in an amount ranging from 7 to 12 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 0.5 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; a fragrance in an amount ranging from 5 to 15 % by weight of the formulation and a coloring agent in an amount ranging from 0.2 to 2 % by weight of the formulation.
[00039] In an embodiment, coloring agent(s) includes but not limited to lemon yellow color. However, a person skilled in the art would appreciate that any other coloring agent(s) can be utilized to serve the intended purpose.
[00040] In an embodiment, fragrance(s) includes but not limited to sun bright. However, a person skilled in the art would appreciate that any other fragrance(s) can be utilized to serve the intended purpose.
[00041] In an embodiment, the formulation further includes a surfactant and a pH adjusting agent.
[00042] In an embodiment, surfactant(s) includes but not limited to sodium coco sulfate, alpha olefin sulfonate, polyethylene glycol, polyvinyl pyrrolidone, narrow-range ethoxylate, octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, nonoxynols, Triton X-100, Polyethoxylated tallow amine, Cocamide monoethanolamine, Cocamide diethanolamine, Glycerol monostearate, Glycerol monolaurate, Sorbitan monolaurate, Sorbitan monostearate, Sorbitan tristearate, Tween 20, Tween 40, Tween 60, Tween 80, Alkyl polyglycoside, Decyl glucoside, Lauryl glucoside, Octyl glucoside, cetylpyridinium, alkyl-dimethyl dichlorobenzene ammonium, dequalinium and phenamylinium chlorides, cetrimonium and cethexonium bromides, hydroxypropyl methyl cellulose, xanthan gum, guar gum, sodium starch glycolate, polyethylene glycol, polyvinyl pyrrolidone, methyl cellulose and combination thereof. However, a person skilled in the art would appreciate that any other surfactant(s) can be utilized to serve the intended purpose.
[00043] In an embodiment, the pH adjusting agent(s) include but not limited to conventionally known acidifying and/or basifying agents. However, a person skilled in the art would appreciate that any other pH adjusting agent(s) can be utilized to serve the intended purpose.
[00044] In an embodiment, the concentrate formulation is a ready to use formulation. In an embodiment, the formulation is a multi-functional, self-dissolving concentrate that easily dissolves in a solvent such as water without requiring any external agitation.
[00045] In an embodiment, the formulation forms a low viscosity solution in water. In an embodiment, 15 to 40 ml of the concentrate formulation is to be dissolved/mixed with 200 to 1000 ml of water. In an embodiment, 25 ml of the concentrate formulation is dissolved/mixed with 500 ml of water.
[00046] In an embodiment, the concentrate formulation has a viscosity in the range of about 0.5 cps to 8.0 cps in water, when 25 ml of concentrate formulation is dissolved/mixed with 500 ml of water.
[00047] In an embodiment, the concentrate formulation has neutral to slightly acidic pH ranging from 3.0 to 8.0, preferably, from 4.0 to about 6.9, most preferably, from 5.0 to about 6.9.
[00048] In an embodiment, the formulation reduces or inhibits the growth of or kills microbes, wherein the microbes include but not limited to green and blue-green algae, gram negative and gram positive bacteria, enveloped and non-enveloped viruses, as well as fungi, including molds and yeasts.
[00049] In an embodiment, the floor cleaning concentrate formulation kills microorganisms in less than 21 seconds.
[00050] In an embodiment, the floor cleaner concentrate formulation can be used for all kinds of floors selected from sheet floors (e.g., vinyl flooring, linoleum or rubber sheeting), vinyl composite tiles, luxury vinyl tiles, vinyl asbestos tiles, rubber tiles, cork and synthetic sports floors, and non-resilient substrates such as concrete, stone, marble, wood, bamboo, ceramic tile, grout, Terrazzo and other poured or “dry shake” floors, epoxy, polyvinyl chloride (PVC), and methyl methacrylates (MMA),cement, linoleum, rubber, and the like.
[00051] In an embodiment, the floor cleaner concentrate formulation can be used as a hard surface cleaner, a kitchen cleaner, a bathroom cleaner, a toilet bowl cleaner, a glass cleaner, a floor cleaner, a carpet cleaner, a biocidal cleaner, an all-purpose general cleaner, and the like.
[00052] In an embodiment, the formulation can be used to clean the surfaces using a variety of conventional methods and tools, including spraying (e.g., with a trigger sprayer, pump sprayer, aerosol, or with an onboard spray device located on a mop), squirting, brushing, flat or string mopping, using a machine such as a floor cleaning machine, mop application, preferably flat mopping, and the like.
[00053] Another aspect of the present disclosure relates to a method of preparing a floor cleaning concentrate formulation, said method including the steps of: preparing a first pre-mix by uniformly mixing benzalkonium chloride (BKC), hydrogen peroxide and foam stabilizer; preparing a second pre-mix by grinding povidone iodine, an auxiliary antimicrobial agent, preservative and optionally, a coloring agent and a fragrance; and mixing the first pre-mix and the second pre-mix to prepare the concentrate formulation.
[00054] In an embodiment, the foam stabilizer selected from cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), caprylyl, coco glucoside, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroylsarcosinate, sodium pareth sulfate, sodium stearate, alpha olefin sulfonate and combination thereof.
[00055] In an embodiment, the auxiliary antimicrobial agent selected from Tulsi Extract, Neem Extract, tea tree oil, peppermint oil, cinnamon oil, thyme oil, lemon extract, vinegar, eucalyptus oil, clove oil, lemon, lavender, oregano and combinations thereof.
[00056] In an embodiment, the preservative is selected from tetrasodium EDTA, citric acid and combinations thereof.
[00057] In an embodiment, the floor cleaning concentrate formulation includes: (a) a cleansing and antimicrobial agent in an amount ranging from 50 to 80 % by weight of the formulation, said agent including a combination of benzalkonium chloride (BKC), hydrogen peroxide and povidone iodine; and (b) the remainder being an auxiliary agent. In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation, hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation and povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation.
[00058] In an embodiment, the auxiliary agent includes: (i) a foam stabilizer selected from cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), caprylyl, coco glucoside, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroylsarcosinate, sodium pareth sulfate, sodium stearate alpha olefin sulfonate and combination thereof; (ii) an auxiliary antimicrobial agent selected from tulsi extract, neem extract, tea tree oil, peppermint oil, cinnamon oil, thyme oil, lemon extract, vinegar, eucalyptus oil, clove oil, lemon, lavender, oregano and combinations thereof; (iii) a preservative selected from tetrasodium EDTA, citric acid and combinations thereof; and (iv) optionally a fragrance and a coloring agent.
[00059] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation; hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation; povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 5 to 15 % by weight of the formulation; citric acid in an amount ranging from 5 to 15 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 1 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 1 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 1 % by weight of the formulation; a fragrance in an amount ranging from 1 to 20 % by weight of the formulation; and a coloring agent in an amount ranging from 0.2 to 3 % by weight of the formulation.
[00060] In an embodiment, the formulation includes: benzalkonium chloride (BKC) in an amount ranging from 35 to 45 % by weight of the formulation; hydrogen peroxide in an amount ranging from 17 to 27 % by weight of the formulation; povidone iodine in an amount ranging from 3 to 7 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 7 to 12 % by weight of the formulation; citric acid in an amount ranging from 7 to 12 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 0.5 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; a fragrance in an amount ranging from 5 to 15 % by weight of the formulation and a coloring agent in an amount ranging from 0.2 to 2 % by weight of the formulation.
EXAMPLES
[00061] The present disclosure is further explained in the form of following examples. However, it is to be understood that the foregoing examples are merely illustrative and are not to be taken as limitations upon the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the scope of the invention.
[00062] Example 1: An exemplary concentrate formulation was prepared, composition whereof is provided in Table 1 below.
TABLE 1: A FLOOR CLEANING CONCENTRATE FORMULATION
Concentrate Liquid Formulation
S. No. Ingredients Amount
(% w/w)
1 Benzalkonium Chloride (BKC) 41.85
2 Hydrogen Peroxide 21.93
3 Cocamide Diethanolamine (CDEA) 9.97
4 Citric Acid 9.97
5 Sun BrightFragrance 9.95
6 Povidone iodine 4.98
7 Lemon Yellow Color 0.80
8 Tetrasodium EDTA 0.25
9 Tulsi Extract 0.15
10 Neem Extract 0.15
Total 100
[00063] Method of preparation: All the ingredients, as per the composition provided in Table 1 above, were weighed accurately and the formulation was prepared as follow: A first pre-mix was prepared by uniformly mixing benzalkonium chloride (BKC), hydrogen peroxide and CDEA. A second pre-mix was prepared separately by grinding povidone iodine, lemon yellow color, tulsi extract, neem extract, citric acid, tetrasodium EDTA and sun bright fragrance, till a uniform mixture was obtained. First pre-mix was added into the second pre-mix under constant stirring till a uniform mixture of the concentrate formulation was obtained. The obtained concentrate was stored in a tightly closed container at room temperature for further use.
[00064] ANTIMICROBIAL ACTIVITY
[00065] The antimicrobial activity of the above prepared formulation was studied according to ASTM-E 2315-016 and the process used therefor was: about 10 ml of the sample formulation was taken with respect to individual test culture. About 100 µl of adjusted test cultures were inoculated in individual sample prepared. A contact time of about 20-60 seconds were taken. Using the pour plate technique about 1 ml of above prepared sample was pipette to triplicate petri-dishes for each dilution prepared by serial dilution technique, and then 20ml of melted SCDA was added. The Petri dishes were incubated at 30-35°C for 72 hours. The colonies on the petri-dishes were counted with the help of colony counter and the observations were recorded in cfu (colony forming unit). Please note that the petri-dish showing a number of colonies; 15-300 for bacterial and fungal of 15-150 range were used for performing the result calculation.

LOG REDUCTION CALCULATION
The effectiveness of the disinfection procedure against each challenge organism was calculated for each product as a log10 reduction as follows:
Log10 reduction per product= Log10 [Positive Control Count] – Log10 [Test Surface Count]
The log reduction will be rounded to the nearest 0.1 value. For each product concentration and each experimental condition, calculate and recorded the decimal log reduction separately.

ACCEPTANCE CRITERIA
In case of surface challenge test positive control should yield 50 to 200% recovery of the microorganism in comparison to inoculums added. Bactericidal, Fungicidal or Yeasticidal activity on surfaces for general purposes is characterized by the concentration of the tested product.
1) Agents should demonstrate a 3 log reduction of vegetative test organisms and mold spores.
2) Agents should demonstrate sporicidal activity of 2 log reduction for B. subtilis spores and a 3 log reduction for A. brasiliensis spores.
TABLE 2: ANTI-MICROBIAL ACTIVITY OF FLOOR CLEANING CONCENTRATE FORMULATION
S. N.
Organism
Contact time
% Reduction
1 Candida albicans(MTCC
227) 20 sec 99.99%
30 sec 99.99%
60 sec 99.99%
2 Staphylococcus aureus(ATCC 6538) 20 sec 99.99%
30 sec 99.99%
60 sec 99.99%
3 Staphylococcusepidermidis(ATCC12228) 20 sec 99.99%
30 sec 99.99%
60 sec 99.99%
4 Escherichia coli(ATCC 8739) 20 sec 99.99%
30 sec 99.99%
60 sec 99.99%
5 Salmonella abony(NCTC6017) 20 sec 99.99%
30 sec 99.99%
60 sec 99.99%
6 Aspergillus brasilensis(ATCC 16404) 20 sec 99.99%
30 sec 99.99%
60 sec 99.99%
7 Listeria monocytogenes(ATCC 19111) 20 sec 99.99%
30 sec 99.99%
60 sec 99.99%
COMPARATIVE ANTI-MICROBIAL ACTIVITY AGAINST PSEUDOMONAS AERUGINOSA
[00066] In a well diffusion method, the anti-microbial activity of the individual ingredients, their combinations (as specified in Table 3) and the formulation prepared herein above (provided in Table 1) was studied and compared. Briefly, about 100 ul of components/formulations were incubated against the pseudomonas aeruginosa for about 12-14 hours at 37°C, and a zone antimicrobial inhibition was analysed.
Table 3: Comparative anti-microbial activity against pseudomonas aeruginosa
Components/formulation Zone of inhibition
(in cm)
Hydrogen Peroxide (0.412%) 2.5
Povidone Iodine (0.103%) 2.7
Benzalkonium Chloride (0.84%) 2.5
Povidone Iodine (0.103%) + BKC (0.84%) 2.7
Hydrogen Peroxide (0.412%) + BKC (0.84%) 2.9
Hydrogen Peroxide (0.412%) + Povidone Iodine (0.103%) + BKC (0.84%) 3.0
Formulation (as in Table 1) 3.0
[00067] It could be noted, albeit surprisingly, that in comparison to the anti-microbial efficacy of Hydrogen Peroxide, Povidone Iodine, Benzalkonium Chloride alone or binary mixtures thereof, the ternary combination of Hydrogen Peroxide, Povidone Iodine, Benzalkonium Chloride affords enhanced anti-microbial activity owing to existence of synergism therebetween, plausibly owing to functional reciprocity therebetween.
[00068] The foregoing description of the specific embodiments will so fully reveal the general nature of the embodiments herein that others can, by applying current knowledge, readily modify and/or adapt for various applications such specific embodiments without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. Therefore, while the embodiments herein have been described in terms of preferred embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modification within the scope of the embodiments as described herein.

ADVANTAGES
[00069] The present disclosure provides a floor cleaning formulation that may overcome the limitations associated with the conventional formulation.
[00070] The present disclosure provides a floor cleaning formulation that exhibits excellent cleansing and antimicrobial properties.
[00071] The present disclosure provides a formulation that exhibits excellent solubility, foam stability and disinfectant activity.
[00072] The present disclosure provides a formulation that exhibits synergistic activity /functional reciprocity.
[00073] The present disclosure provides a formulation that is safe in use.
[00074] The present disclosure provides a formulation that is easy to prepare and is economical.

CLAIMS:1. A floor cleaning concentrate formulation, said formulation comprising:
(a) a cleansing and antimicrobial agent in an amount ranging from 50 to 80 % by weight of the formulation, said agent comprising a combination of benzalkonium chloride (BKC), hydrogen peroxide and povidone iodine; and
(b) the remainder being an auxiliary agent.
2. The formulation as claimed in claim 1, wherein the formulation comprises: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation, hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation and povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation.
3. The formulation as claimed in claim 1, wherein the auxiliary agent comprises:
(i) a foam stabilizer selected from cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), caprylyl, coco glucoside, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroylsarcosinate, sodium pareth sulfate, sodium stearate alpha olefin sulfonate and combination thereof;
(ii) an auxiliary antimicrobial agent selected from tulsi extract, neem extract, tea tree oil, peppermint oil, cinnamon oil, thyme oil, lemon extract, vinegar, eucalyptus oil, clove oil, lemon, lavender, oregano and combinations thereof;
(iii) a preservative selected from tetrasodium EDTA, citric acid and combinations thereof; and
(iv) optionally a fragrance and a coloring agent.
4. The formulation as claimed in claim 1, wherein the formulation comprises: benzalkonium chloride (BKC) in an amount ranging from 35 to 55 % by weight of the formulation; hydrogen peroxide in an amount ranging from 14 to 30 % by weight of the formulation; povidone iodine in an amount ranging from 1 to 10 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 5 to 15 % by weight of the formulation; citric acid in an amount ranging from 5 to 15 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 1 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 1 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 1 % by weight of the formulation; a fragrance in an amount ranging from 1 to 20 % by weight of the formulation; and a coloring agent in an amount ranging from 0.2 to 3 % by weight of the formulation.
5. The formulation as claimed in claim 1, wherein the formulation comprises: benzalkonium chloride (BKC) in an amount ranging from 35 to 45 % by weight of the formulation; hydrogen peroxide in an amount ranging from 17 to 27 % by weight of the formulation; povidone iodine in an amount ranging from 3 to 7 % by weight of the formulation; cocamide diethanolamine (CDEA) in an amount ranging from 7 to 12 % by weight of the formulation; citric acid in an amount ranging from 7 to 12 % by weight of the formulation; tetrasodium EDTA in an amount ranging from 0.1 to 0.5 % by weight of the formulation; tulsi extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; neem extract in an amount ranging from 0.05 to 0.5 % by weight of the formulation; a fragrance in an amount ranging from 5 to 15 % by weight of the formulation and a coloring agent in an amount ranging from 0.2 to 2 % by weight of the formulation.
6. A method of preparing a floor cleaning concentrate formulation, said method comprising the steps of: preparing a first pre-mix by uniformly mixing benzalkonium chloride (BKC), hydrogen peroxide and a foam stabilizer; preparing a second pre-mix by grinding povidone iodine, an auxiliary antimicrobial agent, a preservative and optionally, a coloring agent and a fragrance; and mixing the first pre-mix and the second pre-mix to prepare the formulation.
7. The method as claimed in claim 6, wherein the foam stabilizer is selected from cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), caprylyl, coco glucoside, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroylsarcosinate, sodium pareth sulfate, sodium stearate, alpha olefin sulfonate and combination thereof
8. The method as claimed in claim 6, wherein the auxiliary antimicrobial agent is selected from tulsi extract, neem extract, tea tree oil, peppermint oil, cinnamon oil, thyme oil, lemon extract, vinegar, eucalyptus oil, clove oil, lemon, lavender, oregano and combinations thereof;
9. The method as claimed in claim 6, wherein the preservative is selected from tetrasodium EDTA, citric acid and combinations thereof.