Field of Invention:
Present invention relates to a culture medium for formulation for promoting growth in culture or in field. More particularly, the culture medium refers to a process for formulation comprising plant parts of weeds Parthenium hysterophorus or Lantana camara.

Background of Invention:
The general purpose media for bacterial growth like Nutrient agar involves ingredients of animal origin. Thus their extraction and use in media formulation may lead to ethical and environmental issues. In addition their formulation involves processes like enzyme digestion which significantly enhances their overall cost.
While being used as a biofertilizer, the other alternative like composting takes 30-40 days. The seaweeds which are being used as biofertilizers are available in liquid form and their collection may also be a costly affair adding to the overall cost.
Lokesh Dubey, Megha Dubey and Princy Jain (2015). Role of green manuring in Organic farming. Plant Archives. Vol. 15 No. 1, ISSN 0972-5210, pp. 23-26 discloses that green manure can be prepared by mixing plant leaf and weeds and the same is spread in the field as a biofertilizer prior to 4-6 week of planting the next crop. However, the present invention discloses a process wherein the formulation can be prepared just in 4 days and does not involve batch variation.
Nagavallemma KP. Wani SP. Stephane Lacroix. Padmaya VV. Vineela C. Babu Rao M. and Sahrawat KL. 2004. Vermicomposting: Recycling Wastes into Valuable organic fertilizer. Global theme on Agrecosystems Report no.8, Patancheru 502324, Andra Pradesh, India. International crops research institute for the Semi-Arid Tropics. 20 pp. discloses commercially available alternative of our invention as biofertilizer is Vermicompost. However, for preparation of vermicompost various raw material such as dry organic waste, dung slurry, rock phosphate, earthworm, agriculture waste etc. are required and again its complete preparation takes about 2 months.
Thus, there arises a need to develop a formulation which is prepared in a shorter time period and is made up of natural ingredients.

Summary of Invention:
In this disclosure, whenever a composition, an element or a group of elements is preceded with the transitional phrase "comprising", it is understood that we also contemplate the same composition, element or group of elements with transitional phrases "consisting essentially of”, "consisting", "selected from the group of consisting of, "including", or "is" preceding the recitation of the composition, element or group of elements and vice versa.
According to an aspect of the invention, there is provided a formulation for promoting growth in culture or in field, said formulation prepared from plant parts of weeds Parthenium hysterophorus or Lantana camara.
Yet according to another embodiment of the invention the plant part is leaf.
Yet according to another embodiment of the invention the formulation promotes growth of bacteria, fungi in a culture medium and promotes growth of plants in a field.
Yet according to another embodiment of the invention the formulation is in form of a powder.
Yet according to another embodiment of the invention there is provided a process for preparation for formulation of any of the preceding claim, said process comprises:
(i) grinding air dried leaves of weeds Parthenium hysterophorus or Lantana camara by grinding;
(ii) treating the grinded leaves of (i) with an oxidizing agent;
(iii) grinding the treated powder of (ii) to a fine powder;
(iv) dissolving fine powder of (iii) in distilled water followed by addition of an alkali to maintain a pH of 7.
(v) dehydrating the powder of (iv) followed by grinding to obtain the powder for formulation.

(vi) dehydrating the powder of (iv) followed by grinding to obtain the powder for formulation.

Yet according to another embodiment of the invention the alkali is NaOH.
Yet according to another embodiment of the invention, the oxidizing agent is UV treated 30% H2O2.

Yet according to another embodiment of the invention the leaf powder is treated with H2O2 for a period of 6 to 12 hours at a temperature ranging from 35 ºC to 65 ºC.

Yet according to another embodiment of the invention for grinded particle size in step (i) is less than 3mm, the range of amount of grinded leaves to H2O2 is 2: 1 to 1:3 w/v.

Yet according to another embodiment of the invention for grinded particle size in step (i) is greater than 3mm the ratio of grinded leaves to H2O2 is 1:2 to 1:5 w/v.

Detailed description of Invention:
The invention makes use of the weed (plant material) for development of a powdery product that can be used as a growth medium for laboratory cultivation of microorganism as well as a biofertilizer.
The invention provides a formulation for promoting growth in culture or in field, said formulation prepared from plant parts of weeds Parthenium hysterophorus or Lantana camara.
Leaves were collected from Parthenium hysterophorus or Lantana camara weeds and air dried, followed by crushing to a powder by a grinder. The leaf powder was then treated with UV activated H2O2 for overnight at a temperature ranging from 35 ºC to 60 ºC, preferably 50 ºC.
According to an embodiment of the invention the UV activated H2O2 is 30%. Hydrogen peroxide treated powder was further grinded into fine powder by mortar pestle and the fine powder was dissolved in distilled water and it pH was maintained to 7 by a alkali solution, for example NaOH to form a liquid, which is completely dehydrated to powder form in a hot air oven. The solid mass is again crushed into powder form by mortar pastel. This powder is the final product to be used as a media for microbial growth as well as a biofertilizer.
According to an embodiment of the invention powder has particles in size range of 7µm to 114 µm.
While making a powder of the leaves, if the powder size leafs particle size more than 3mm, in this case, the volume of H2O2 for leaf chunk treatment require more for example 20g leaf chunk of 2.5mm is required 10 ml of H2O2. If leaf chunk size larger than 3mm the H2O2 volume increases up to 10 ml. According to an embodiment of the invention, the ratio of leaves to 30% UV activated H2O2 is 20gm of weed powder to 10ml of 30% UV activated H2O2.

Experiment:
Leaves of weeds Parthenium or Lantana camara were collected and air dried. Air dried leaves were crushed to powder by a grinder. 20g of this leaf powder was then treated with 10 ml of UV activated 30% H2O2 for overnight at 50ºC. Hydrogen peroxide treated powder was further grinded into fine powder by mortar pestle and the fine powder was dissolved in distilled water and it pH was maintained to 7 by NaOH solution. The liquid was completely dehydrated to solid form in hot air oven. The solid mass is again crushed into powder form by mortar pastel. This powder is the final product to be used as a media for microbial growth as well as a biofertilizer.

Further areas of applicability of the present invention will become apparent from the detailed description provided hereinafter. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
While the embodiments of the present invention have been disclosed above, but its use is not limited to the description set forth and described embodiments, which can be applied to various fields suitable for the present invention, for the person skilled in the art, can be easily realized a further modification, thus without departing from the generic concept claims and equivalents as defined by the scope of the present invention is not limited to the specific details shown and described herein with legend.

We claim:
1. A formulation for promoting growth in culture or in field, said formulation prepared from plant parts of weeds Parthenium hysterophorus or Lantana camara.

2. The formulation as claimed in claim 1, wherein the plant part is leaf.

3. The formulation as claimed in claim 1, wherein the formulation promotes growth of bacteria, fungi in a culture medium and promotes growth of plants in a field.

4. The formulation as claimed in claim 2, wherein the formulation is in form of a powder.

5. A process for preparation for formulation of any of the preceding claim, said process comprises :

(i) grinding air dried leaves of weeds Parthenium hysterophorus or Lantana camara, by grinding;
(ii) treating the grinded leaves of (i) with an oxidizing agent;
(iii) grinding the treated powder of (ii) to a fine powder;
(iv) dissolving fine powder of (iii) in distilled water followed by addition of an alkali to maintain a pH of 7;
(v) dehydrating the powder of (iv) followed by grinding to obtain the powder for formulation.

6. The process as claimed in claim 5, wherein the alkali is NaOH.

7. The process as claimed in claim 5, the oxidizing agent is UV treated 30% H2O2.

8. The process as claimed in claim 7, wherein the leaf powder is treated with H2O2 for a period of 6 to 12 hours at a temperature ranging from 35ºC to 65ºC.

9. The process as claimed in claim 7, wherein for grinded particle size in step (i) is less than 3mm, the range of amount of grinded leaves to H2O2 is 2: 1 to 1:3 w/v.

10. The process as claimed in claim 7, wherein for grinded particle size in step (i) is greater than 3mm the ratio of grinded leaves to H2O2 is 1:2 to 1:5 w/v.