Description:FIELD OF THE INVENTION
This invention relates to a formulation of biopesticide having an enhanced ability to improve plant growth and/or promoting plant health, by exhibiting high activity against fungal disease, particularly Cercospora malayensis in okra plant.
BACKGROUND OF THE INVENTION
In the realm of crop protection, there is a constant and pressing need to explore innovative methods and solutions that can enhance plant health and promote optimal growth. After all, the fundamental principle behind successful crop cultivation is quite straightforward; healthier plants invariably lead to higher yields and/or improved quality of the plant itself or the products derived from it. This simple yet profound truth underscores the critical importance of investing in research, development, and the implementation of cutting-edge strategies to safeguard and nurture the vitality of agricultural crops.
Pesticides and fungicides have become ubiquitous in agricultural practices worldwide, employed as a means to combat harmful pests and diseases. However, the synthetic chemical compounds utilized in these conventional pest control methods often lack specificity, inadvertently impacting non-target organisms, including beneficial species that play crucial roles in ecological balance. Repeated use of synthetic pesticides or fungicides can lead to resistant pests or microorganisms. Mancozeb, a dithiocarbamate non-systemic fungicide is commonly used to treat Cercospora malayensis. These Cercospora strains are often cross-resistant to other compounds with the same mechanism. This renders the active ingredient ineffective. Developing new active ingredients with different mechanisms is difficult and costly.
Furthermore, the inherent chemical nature of these substances can render them toxic and resistant to biodegradation, leading to the accumulation of harmful residues, particularly in food products. Consumers across the globe are growing increasingly aware of the potential environmental and health-related risks associated with the presence of these chemical residues, fueling a rising demand for more sustainable and eco-friendly pest management solutions.
As a result, there is mounting pressure on agricultural stakeholders to explore alternative approaches that can effectively control pests while minimizing the reliance on synthetic pesticides and fungicides. Navigating this delicate balance between managing food production requirements and implementing effective, yet environmentally responsible, pest control strategies has become a pressing concern. To addressing this concern, requires a multifaceted approach that considers the needs of both producers and consumers, while prioritizing the protection of natural ecosystems and human health. Despite these challenges, the inherent benefits of biopesticides, such as their eco-friendly nature, reduced environmental impact, and potential for sustainable agriculture, make them a highly attractive option.
U.S. Patent Publication US 2011/0319430 discloses certain fungicidal pyrazoles, but does not disclose the fungicidal mixtures of the present invention.
US2009/156592 discloses certain fungicidal azocyclic amides and their use in controlling plant diseases caused by fungal plant pathogens but does not disclose the fungicidal mixtures of the present invention.
DK2809157T3 titled “FUNGICIDE PYRAZOL MIXTURES” relates to a fungicidal composition (i.e. combination, mixture) comprising at least one compound selected from the stereoisomers), A/-oxides, and salts thereof:
JP6923437B2 titled “Composition containing recombinant Bacillus cells and another biopesticide’ discloses a synergistically effective amount of
a) (i) enzymes involved in the production or activation of plant growth-stimulating compounds and enzymes or pathogens that degrade or modify the nutrient sources of bacteria, fungi or plants. At least one plant growth-stimulating protein or peptide selected from the group consisting of proteins or peptides that protect the enzyme; and (ii) recombination that expresses a fusion protein containing a target sequence that localizes the fusion protein to the outer membrane of Bacillus cells. Outer membrane-producing Bacillus cells; and
b) All of the further different specific biopesticides and / or individual strains disclosed herein that exhibit activity against insects, mites, nematodes and / or phytopathogens.
It relates to a composition comprising a variant of a particular strain of a bacterium disclosed herein having distinctive properties and / or at least one metabolite produced by an individual strain.
The use of biopesticides, also known as biological control agents (BCAs), presents a compelling alternative to conventional fertilizers and synthetic pesticides. While in some cases the effectiveness of BCAs may not match that of traditional chemical-based products, especially under conditions like highly infectious conditions, they offer distinct advantages that warrant serious consideration. One of the key limitations of biopesticides is that at lower dosage levels, their performance and the efficacy of their mutant strains or metabolic byproducts may not be entirely satisfactory.
In addition to the prior art above, introducing novel fungicide combinations - the strategic combination of existing fungicides has proven to be an effective strategy. Certain rare fungicide combinations can demonstrate a synergistic effect, providing commercially significant levels of plant disease control. The advantages of specific fungicide combinations can vary depending on factors such as the plant species, the target disease, and whether the plants are treated before or after infection by the fungal pathogen.
Consequently, there is a pressing need to further innovate and develop new, advantageous fungicide combinations that can provide a diverse range of options to best address the specific needs of plant disease control. This underscores the need for continued research and development to enhance the capabilities of these biological solutions.
OBJECTIVE OF THE INVENTION
The principal objective of the invention is to provide a formulation of biopesticide having an enhanced ability to improve plant growth and/or promoting plant health, by exhibiting high activity against fungal disease, particularly Cercospora malayensis caused diseases in okra plant.
STATEMENT OF THE INVENTION
This invention relates to a formulation of biopesticide having an enhanced ability to improve plant growth and/or promoting plant health, by exhibiting high activity against fungal disease. The formulation comprising a) Annona reticulata leaf extract as the active ingredient 40%w/w, b) Sapindus mukorossi (surfactant) 10%w/w c) Calotropis gigantea (thickener) 5%w/w, d) Glycine (antifreeze) 5%w/w, and e) Demineralised water 40%w/w. This synergistic formulation of biopesticide has demonstrated remarkable potential for controlling Cercospora leaf spot disease in crop plants. In a series of experiments, all the treatments evaluated showed statistically significant differences in the population of individual okra plants compared to the untreated control at 10 days after application. Notably, the 50% concentration of the formulation exhibited statistically superior efficacy, underscoring its potent and targeted action against the targeted fungal pathogens. The versatility and adaptability of this synergistic biopesticide formulation make it a promising solution for addressing a wide array of fungal challenges faced by crop growers.
BRIEF DESCRIPTION OF DRAWINGS
Figure 1: depicts a thick layer of creamy coloured fungal hyphae of Cercospora malayensis fungi as developed on the PDA medium.
Figure 2: depicts the Fungal hyphae observed by compound microscope under 100X magnification
Figure 3: depicts Fungal hyphae observed in SEM (A) Image of sample treated with Annona reticulata leaf extract (B) Image of sample without treatment.
Figure 4: Zone of inhibition of fungal hyphae as observed at 25%,50%,75%,100%
Figure 5: depicts Soxhlet apparatus used for extraction
Figure 6: Farmer spraying the composition on the okra field
Figure 7: depicts A) picture of infected plant and B) picture of plant after spraying
Figure 8: depicts observance of effectiveness of biofungicides on lady’s finger at field level (A. more infected leaf, B. Minimize the infection compared to A, C. More minimize growth of fungus as compared to B, D. More inhibition of fungal spore is observed).
DETAILED DESCRIPTION OF THE INVENTION
Accordingly, the present invention provides a synergistic formulation of biopesticide having an enhanced ability to improve plant growth and/or promoting plant health, by exhibiting high activity against fungal disease.
The control of plant diseases caused by fungal plant pathogens is a critical component in achieving high crop efficiency and productivity. Damage to ornamental, vegetable, field, cereal, and fruit crops can result in significant reductions in yield, ultimately leading to increased costs for consumers. These plant diseases can be particularly challenging to manage, as they often develop resistance to commercially available fungicides. To address this issue, there is a continued need for the development of new, more effective fungicidal compounds that are less costly, less toxic, and environmentally safer, with different mechanisms of action and this invention is addressing that need to the stake holders.
The term “plant propagation material” is understood to denote generative parts of a plant, such as seeds, vegetative material such as cuttings or tubers, roots, fruits, tubers, bulbs, rhizomes and parts of plants, germinated plants and young plants which are to be transplanted after germination or after emergence from the soil. These young plants may be protected before transplantation by a total or partial treatment by immersion.
It is to be understood that the singular forms “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise.
It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, steps or components but does not preclude the presence or addition of one or more other features, steps, components or groups thereof.
The term “fungi” as used herein, includes all organisms in the class “Ascomycota”.
The term “Fungicidal” as used herein, refers to the ability of a pesticide to increase mortality or inhibit growth rate of fungi.
The terms “weight percent”, “WT-%”, “percent by weight”, “% by weight” and variations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the composition and multiplied by 100. It is understood that, as used here, “percent”, “%” and the like are intended to be synonymous with “weight percent”, “wt. %”, etc.
The term “synergist” as used herein refers to a material which enhances the activity of other materials such as fungicides so that the overall activity of the composition is greater than the sum of the individual ingredient’s activities.
Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a way similar to one or more embodiments and/or in combination with or instead of the features of the other embodiments.
Annona reticulata belongs to the Annonaceae family, and commonly called as Custard Apple. It has a few medical properties like anthelmintic, analgesis, anti-inflammatory, antipyretic, Wound healing, cytotoxic and antimicrobial impacts. It is broadly disseminated with phytochemicals like tannins, alkaloids, phenols, glycosides, flavonoids and steroids. It is very useful for controlling many insects and microbes. Herein we have focused a bio fungicide prepared from leaf extract of Annona reticulata against Cercospora leaf spot disease. The casual organism of this disease is Cercospora malayensis, which is primarily developed in the lower surface of leaves as an irregular spots. The Annona leaf extract inhibits the Cercospora malayensis by reducing the number of fungal spores in leaf.
The present invention thus provides a fungicidal composition comprising
a) Annona reticulata leaf extract as the active ingredient 40%w/w
b) Sapindus mukorossi (surfactant) 10%w/w
c) Calotropis gigantea (thickener)5%w/w
d) Glycine (antifreeze) 5%w/w, and
e) Demineralised water 40%w/w.
In an embodiment, the fungicidal composition of the present invention comprising the said active ingredients exhibit synergistic effect in the controlling of the fungi in agriculture crops, particularly Cercospora malayensis in okra plant.
In an embodiment, the fungicidal composition of the present innovation is formulated in the form of a suspension concentrate comprising a dispersing agent, an antifreeze agent and a thickener.
In a preferred embodiment the dispersing agent is Sapindus mukorossi , particularly in the ratio of 10%w/w.
In an embodiment the thickening agent is Calotropis gigantean, particularly in the ratio of 5%w/w.
In other embodiment the antifreeze agent is Glycine, particularly in the ratio of 5%w/w.
In a further embodiment, the insecticidal composition is formulated in a form selected from the group comprising water soluble concentrates (SL), emulsifiable concentrates (EC), emulsion (EW), micro-emulsion (ME), suspension concentrates (SC), oil based suspension concentrates (OD), flow able suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), granules(GR), encapsulated granules (CG), fine granules (FG), macro granules (GG), dry flowables (DF), aqueous suspo-emulsion (SE), Capsule suspension (CS) and micro granules (MG).
In a preferred embodiment, the fungicidal composition of the present invention is formulated as a suspension concentrate.
While the invention described in this disclosure is amenable to various modifications and alternative forms, the examples provided in the experiments serve as to only illustrate its effectiveness. However, the intention is not to limit the invention solely to these specific examples. Rather, the invention is intended to encompass a broad range of modifications, equivalents, and alternative embodiments that fall within the scope of the detailed description provided herein
EXAMPLE-1
ISOLATING AND CULTURING PHYTO PATHOGEN (Cercospora malayensis)
Materials required:
Infected leaf, forceps, potato, dextrose, Agar, distilled water, laminar air flow, incubator, conical (Erlenmeyer) flask, beaker, petri dishes, cotton.
Procedure:
a) Collecting the Cercospora leaf spot disease infected leaves from the locality to laboratory.
b) Potato Dextrose Agar (PDA) medium is preferred to culture mold, yeasts and different mycotic parasites. So, PDA medium is prepared for culturing the collected phytopathogenic fungi.
c) Taking in a conical flask a mixture of 100ml of distilled water and 20gm of potato.
d) Heating the mixture in hot plate until it becomes a semifluid form.
e) Filtering the semifluid obtained to collect the filtrate in a beaker, and is made up to 100ml of solution by adding distilled water.
f) Mixing 2gm dextrose and 1.5gm Agar to the prepared solution in a conical flask for completing the PDA medium and kept for cooling.
g) Cleaning the laminar air flow bench with ethanol.
h) Platting is done by adding 20ml of PDA medium from the conical flask to petri plate(s).
i) After transferring the medium onto the petri plates, they are exposed to UV light in the laminar air flow bench for 2 minutes.
j) PDA medium in the petri plates is allowed to solidify; once solidified, the phytopathogen infected part from the collected leaf is isolated with sterile forceps and inoculated in the PDA medium.
k) All the inoculated PDA medium petri plates are incubated in a BOD incubator at 28+ 1℃ for 2-3 days.
l) A thick layer of creamy coloured fungal hyphae is developed on the PDA medium (Figure-1).
EXAMPLE 2
MICROSCOPIC IDENTIFICATION OF THE PHYTOPATHOGEN
1. COMPOUND MICROSCOPE
Materials required:
Infected leaf, needle, glass slide, safranine, dropper, cover slip, immersion oil, compound microscope.
Procedure:
a) Collecting infected leaf from locality to laboratory.
b) Isolating the phytopathogenic fungi from the leaf by using needle and placing it on clean glass slide.
c) Adding a drop of safranine on the fungus in a glass slide and placing a cover slip over it.
d) Observing under Olympus compound magnifying instrument at oil immersion objective focal point (100X magnification).
e) A stained fungal hyphae is observed, based on micro- morphological features, branched septate hyphae with conidiophores and conidia which were hyaline, and subacute is also observed. The conidia are detached from the conidiophore and spreaded under the coverslip (Figure-2).
2. SCANNING ELECTRON MICROSCOPE (SEM):
Materials required:
Fungal hyphae, cover slip, osmium tetroxide, ethanol, scanning electron microscope.
Procedure:
a) Collecting the fungal hyphae from the prepared fungus in a petri plate.
b) Taking the Fungal hyphae in a cover slip and fixing it with aldehyde followed by osmium tetroxide.
c) Using ethanol for dehydration and drying in a hot air oven for 45min.
d) Placing the dried sample inside the Scanning Electron Microscope (SEM).
e) Observing under SEM, the specimen is exposed to a narrow electron beam from an electron gun, which rapidly moves over or scans the surface of the specimen releasing a shower of secondary electrons and other types of radiations from the specimen surface.
f) Collecting the electrons by a detector, which generates electronic signals.
g) Scanning the signals in the manner of a television system to produce an image on a cathode ray tube.
h) Observing under the instrument with different angles to obtain the better view/photograph.
i) A cultured Cercospora is observed under Scanning Electron Microscope (SEM). The distinct ascospores spreading over the plate is documented (Figures- 3 a&b).
EXAMPLE 3
PREPARATION OF PLANT EXTRACT:
Materials required:
Annona reticulata leaf, mechanical grinder, weighing machine, Soxhlet apparatus, beaker, distilled water, filter paper, Eppendorf tube.
Procedure:
a) Collecting Annona reticulata leaf from the locality and shade drying it for 2-3 days at room temperature.
b) Powdering the dried leaves by using mechanical grinder.
c) Measuring 5gm of powder in the weighing machine.
d) Extracting organic solvent, by using Soxhlet extractor.
e) Taking 150ml of distilled water in a flask, the crude substance is placed in thimble- shaped filter paper and kept in a glass cylinder.
f) The glass cylinder is provided with a siphon tube and an inlet tube. A water condenser is attached to the cylinder at the top. This entire assembly is fitted into the neck of a round bottom flask containing the solvent as in figure 5.
g) Heating the solvent flask (in a water bath or sand bath) vaporizes the solvent and the vapors reach the cylinder through the inlet tube.
h) Any vapor moving upward into the condenser get cooled and drips back to the middle cylinder part, over the thimble.
i) Maintaining a continuous supply of solvent vapors in the cylinder, and ensuring the dissolved organic compounds flows back into the flask.
j) The condensed solvent vapor will accumulate over the thimble till it reaches the siphon tube outlet and once it reaches that level, whole of collected solvent with organic material will be siphoned back to the round bottom flask.
k) Stopping the apparatus and the solution in the flask is distilled to recover the solvent, while the organic compound is left behind.
l) Collecting the extraction in a beaker and heating it to reduce the volume.
m) The extraction volume is reduced to 10ml by evaporation of water and is considered as 100% concentrated sample.
n) Preparing 3 diluted samples in 3 Eppendorf tubes such as 25%, 50%, and 75% using distilled water.
EXAMPLE 4
INVITRO EVALUATION OF BIOFUNGICIDE AGAINST PHYTO PATHOGEN IN AGAR WELL DIFFUSION TECHNIQUE
Materials required:
Gel puncture, prepared sample, laminar air flow, ethanol, cotton, micro pipette, incubator.
Procedure:
a) Cleaning the laminar air flow surface using ethanol with cotton.
b) Taking the Petri plates, where the white-coloured fungal spores are developed in a PDA medium into the laminar air flow.
c) Taking the sterile gel puncture and four wells are made in the petriplates.
d) Then 10µl of prepared sample that is 25%, 50%, 75% and 100% concentrated samples were added to individual well carefully.
e) The Petri dishes are incubated in BOD chamber at 37oC for 48hrs.
f) The clear zone of inhibition (ZOI) by the extract is observed after 48 hours. Maximum ZOI is observed at 75% of the extract (Figure-4). So, the optimum concentration of the extract observed to inhibit the growth of the fungus is 75%. The same concentration is used in future as the optimum concentration.
Fungal species Control 25% 50% 75% 100%
Cercospora malayensis --- 1mm 4.5mm 7mm 7.5mm
EXAMPLE 5
FIELD PREPARATION
a) The seeds of ladies finger is obtained from local market krushi bigyan kendra at Kotturu.
b) Healthy seeds were grown in the green house with soil and fertilizer in the ratio of 3:1.
c) The plants were aligned in a plot size of 12m2 area, with the spacing of 20cm between two adjacent plants and a 20cm between the rows.
d) One month old plants with equal height are selected for studying, by exposing them to the spores of Cercospora at a concentration of 1×106ml and by incubating to develop the disease.
e) The disease symptoms started appearing after 10 days of incubation; as small brown flecks, the fungal spores developed on the lower surface of the leaves.
f) The trail is laid out in 6 blocks of quadrat including control.
EXAMPLE 6
PREPARATION OF FUNGICIDAL COMPOSITION AS SUSPENSION CONCENTRATE (SC)
a. The dispersing agent (Sapindus mukorossi which is also used as a surfactant) were diluted in DM water and mixed with high shear mixer. After mixing, the anti-freezing agent is added in desired amount, followed by addition of Annona reticulata leaf extract. The ingredients were mixed properly to make a homogeneous mixture after with continuous mixing to obtain a homogeneous mass.
b. The homogeneous mass obtained above is wet grinded in Dyno mill.
c. After the wet grinding, 2% aqueous solution of 5% Calotropis gigantea is added under low stirring to obtain a homogenous product with desired viscosity.
In an embodiment, the composition of the present fungicidal formulation is depicted in Table 1.
Table 1: composition of the fungicidal formulation
S.no. Ingredient Quantity
1 Annona reticulata leaf extract 1-40%
2 Sapindus mukorossi (Surfactant) 1-5%
3 Calotropis gigantean (Thickener) 1-2.5%
4 Glycine (Antifreeze) 1-2.5%
5 DM water (Demineralised water) 1-50%

EXAMPLE 7
SAMPLE PREPARATION FOR FIELD APPLICATION
To evaluate the sample in the field, it is prepared as solution of different concentration as follows,
In a 10% concentration of sample, 100 ml of Annona reticulata leaf extract with surfactant, thickener and glycine are mixed with 900ml of water.
In a 50% concentration of sample, 500 ml of Annona reticulata leaf extract with surfactant, thickener and glycine are mixed with 500ml of water.
Similarly, the five different concentrations at 10%, 20%, 30%, 40%, and 50% are prepared.
EXAMPLE 8
FIELD TRIAL
Different concentration of biofungicides samples as prepared such as 10%, 20%, 30%, 40% and 50% are applied to plants with sprayer machine in morning time for 3 times at a regular interval of 5 days each.
a) After sample application, the cure plant count is calculated from the Day After Application (DAA) and the readings are taken as 1 DAA, 5 DAA and 10 DAA. The observation of cure plants is taken from a quadrat with the dimension of 2m2 area.
b) Significant inhibition in the spore germination is observed by different concentrations of biofungicides shown in results (Figures 7 and 8). After third spraying maximum inhibition in the spore germination is found at highest concentration as compared to control which showed least inhibition in spore germination. Results indicated that after 1st, 2nd and 3rd application of biofungicide exhibited minimum disease incidence at 50% concentration.

EXAMPLE-9
EVALUATING THE EFFICACY OF THE PRESENT FUNGICIDAL FORMULATION BY FIELD STUDY
The presently disclosed fungicidal composition is tested for its bio efficacy against Cercospora malayensis on a local variety of okra plant in Gunupur, Odisha. The plants were aligned in a plot size of 12m2 area, with the spacing of 20cm between individual crop plants and a spacing 20cm between the rows. The trial is laid out in a 6 random block including control, design consisting of a one treatment at different concentration.
After application the cure plant count is taken at intervals of five Days After Application (DAA) like 1DAA, 5DAA and 10DAA. The observation of cure okra plant is taken from 2m2 area.
Measured quantity of the substance is added to required volume of water for spraying. The spray tank is filled with ½ the quantity of clean water and balance half with prepared sample. The solution is stirred well before application and thorough coverage is ensured.
Observation of Cure okra plant per 2m2:
A quadrat with the dimension of 2×2 sqm is placed randomly at 5 places in each plot and the cure plant from that area were counted and expressed a number per 2sqm. Data is also converted to percent fungi control and tabulated as follows.
TABLE-2: TREATMENT DETAILS IN OKRA PLANT

S.no.
Treatment details
Dose in %
1DAA
% Control
5DAA
% Control
10DAA
% Control
1 Untreated
2 T1 10% 12 10% 14 10% 15
3 T2 20% 20 20% 22 20% 26
4 T3 30% 40 30% 44 30% 48
5 T4 40% 54 40% 58 40% 62
6 T5 50% 66 50% 69 50% 72

EVALUATION OF BIO-EFFICACY IN OKRA PLANT
Evaluation of bio-efficacy for Cercospora leaf spot disease
The efficacy of fungicide formulation against Cercospora leaf spot disease is judged on the basis of the percent of the number of cure plant per 2×2 sqm and percent of the infected plant per 2×2 sqm. The evaluation is calculated as follow.
Disease control percent = Number of cure plant × 100
Number of infected plants

RESULTS:
The synergistic formulation of a biopesticide has demonstrated remarkable potential for controlling fungal diseases in crop plants. In a series of experiments, all the treatments evaluated showed statistically significant differences in the population of individual okra plants compared to the untreated control at 10 days after application. Notably, the 50% concentration of the formulation exhibited statistically superior efficacy, underscoring its potent and targeted action against the targeted fungal pathogens. The versatility and adaptability of this synergistic biopesticide formulation make it a promising solution for addressing a wide array of fungal challenges faced by crop growers.
, Claims:We claim
1. A fungicidal composition comprising;
a) 40%w/w of Annona reticulata leaf extract as the active ingredient,
b) 10%w/w of Sapindus mukorossi,
c) 5%w/w of Calotropis gigantea,
d) 5%w/w of Glycine, and
e) 40%w/w Demineralised water.
2. The composition as claimed in claim 1, wherein said composition at a concentration of 50% exhibited 72% inhibition rate in controlling the fungal attack in agriculture crops, particularly Cercospora malayensis in okra plant.

3. The composition as claimed in claim 1, wherein said composition is in the form of a suspension concentrate comprising a dispersing agent, an antifreeze agent and a thickener.

4. The composition as claimed in claim 3, wherein said composition the dispersing agent is Sapindus mukorossi.

5. The composition as claimed in claim 3, wherein said composition the thickening agent is Calotropis gigantean.

6. The composition as claimed in claim 3, wherein said composition the antifreeze agent is Glycine.

7. The composition as claimed in claim 1, wherein said composition is formulated in a form selected from the group comprising of water soluble concentrates (SL), emulsifiable concentrates (EC), emulsion (EW), micro-emulsion (ME), suspension concentrates (SC), oil based suspension concentrates (OD), flow able suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macro granules (GG), dry flowables (DF), aqueous suspo-emulsion (SE), Capsule suspension (CS) and micro granules (MG).
8. The composition as claimed in claim 7, wherein said composition is formulated preferably as a suspension concentrate.