Claims:I/We CLAIMS
1. An anti-microbial composition emulsion comprising of
a first mixture; the first mixture having
a Tea Tree oil in the range of 0.5 to 1.5%,
a Rose oil in the range of 0.5 to 1.5%,
a Eucalyptus oil in the range of 0.1 to 1.0%,
a Turmeric Oil in the range of 0.1 to 1.0%, and
a Licorice oil in the range of 0.1 to 1.0%, resulting in an oil blend; and
a second mixture; the second mixture having
Carbopol 940 in the range of 0.1 to 0.5%,
EDTA in the range of 0.01 to 0.05g, and
Purified water;
wherein, the mixtures are obtained using a 3 blade marine impellar stirrer form a vortex having RPM in the range of 800-1200.

2. The composition as claimed in claim 1, wherein the second mixture is a water phase.
3. The composition as claimed in claim1, wherein the first mixture is oil phase comprising all the essential oil in the range of 1 to 6% v/v.
4. The composition as claimed in claim 1, wherein the composition is prepared through the process comprising:

stirring the weighed quality of carbopol 940 for 30 to 60 minutes at 800-1200 RPM in a vessel containing water;

adding EDTA into the carbopol mixture, resulting in a water phase;
adding PG, surface active agents, emulsifiers and benzyl alcohol into the oil phase;
mixing the oily phasewith slow addition into the water phase with continuous stirring at around 800-1200 RPM and blending the mixture for 15 to 20 minutes, resulting in an emulsion;

addingtriethanolamine to neutralizing the solution and formation of oleo gel and maintaining the pH of the composition in the range of 4 to 6;

further stabilizing the composition at room temperature for 6 to 10 hrs.

5. The composition as claimed in claim4, wherein the surface active agents can be selected from but not limited to stearic acid, cetyl alcohol, PEG-100, stearate and glyceryl stearate, cetearylglucoside, polysorbate 20, polysorbate 80 methylcellulose, sodium carboxymethylcellulose, glycerine, bentonite, ceteareth-20, cetyl alcohol, cetearyl alcohol, lanolin alcohol, riconyl alcohol, self-emulsifying wax (e.g., Lipowax P), cetylpalmitate, stearyl alcohol, lecithin, hydrogenated lecithin, steareth-2, steareth-20, and polyglyceryl-2 stearate.

6. The composition as claimed in claim 4, wherein the emulsifiers can be selected from but not limited to glycerine, polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, polyoxyethylene, polyoxypropylene glycol, polyethylene glycol, fatty acid polyethylene glycol, fatty acid polyoxy, polypropylene, sodium laurethsulfates, and specific preservatives, fragrances, and surfactants.
, Description:FIELD OF THE INVENTION
The present invention relates to a natural antimicrobial composition comprising of essential oils. More specifically the present invention relates to a natural composition free from paraffin and other harmful chemical reagents.
BACKGROUND OF THE INVENTION
Cosmetic compositions are known for enhancing the look, feel and health of a user. Cosmetic compositions include skin creams and emulsions having essential oils, that are applied to the skin of the user. Skin creams and emulsions seek to improve the softness of the skin and prevent it from drying out during times of cold, dry weather. Further, skin application products are commonly used for the prevention of acne and to aid in the healing of cuts or burn on the skin of the user and have been used as anti-microbial creams also. Cosmetic compositions are common in everyday life and are generally used on a daily basis by many individuals. Particular needs are thus present for cosmetic compositions made from certain components for use in imparting various desired benefits to the skin of a user. Several studies have been conducted on the possibility of using essential oils for the biological control of microorganisms. Essential oils are degraded faster than synthetic compounds in the environment, and some have increased specificity in favour of beneficial insects. Despite most promising properties, essential oil formulations are difficult to use as a result of problems related to essential oil volatility, low water solubility, and ease of oxidation, and as a result there was a real problem that it was difficult to apply to the target environment. Further, Conventional cosmetic compounds having essential oils use a lot of chemical substances that may damage the skin cells and are also expensive.
US7691419B2 discloses a topical composition for treating skin diseases is provided. The composition comprises calendula, tea tree oil, eucalyptus oil, grape seed oil, and Aloe vera. In some embodiment, the composition may also include beeswax, vegetable oil, vitamin E, rosemary oil, or shea butter or combinations thereof to further enhance its beneficial effects. In some embodiments, the composition may also include herbs. Kits and methods for treatment of skin diseases are also disclosed.

JP6283342B2 discloses an oil-in-water gel composition which can remove or reduce a malodour more favourably than before.SOLUTION: An oil-in-water gel composition for removing or reducing a malodor has an oily component, a hydrophilic gelator and a surfactant, wherein a light transmittance at wavelength 600 nm is 10% or less, a content of the surfactant is 0.1-2 mass% in the composition, and the oily component is an oily aroma component.

Thus the need of the hour is the use of the present invention that overcomes these disadvantages by combining nourishing, natural products that benefit and promote the health of the skin.
OBJECTIVE OF THE INVENTION
The main objective of the present invention is to develop an anti-microbial composition that provides protection up to 6 hours of application.
Yet another objective of the present invention is to provide sanitization of the facial skin by protecting from common pathogenic microbes generally present on the facial skin area. .
Yet another objective of the present invention is to provide skin lightening and skin toning effects.
Yet another objective of the present invention is to provide a soothing and calming effect.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example.
SUMMARY OF THE INVENTION
The present invention discloses an anti-microbial composition emulsion comprising of a first mixture and a second mixture. The first mixture comprises a Tea Tree oil in the range of 0.5 to 1.5%, a Rose oil in the range of 0.5 to 1.5%, a Eucalyptus oil in the range of 0.1 to 1.0%, a Turmeric Oil in the range of 0.1 to 1.0%, and a Licoriceoil in the range of 0.1 to 1.0%, resulting in an oil blend mixture. The second mixture comprises of a Carbopol 940 in the range of 0.1 to 0.5%, EDTA in the range of 0.01 to 0.05g, and purified water. Herein, the mixtures are obtained using a 3 blade marine impeller stirrer form a vortex having RPM in the range of 800-1200. In an embodiment, the second mixture in the composition is a water phase. The first mixture in the composition is an oil phase comprising all the essential oil blends in the range of 1 to 6% v/v. In the present invention, the composition is prepared through the process comprising of stirring the weighed quality of carbopol 940 for 30-60 minutes at 800-1200 RPM in a vessel containing water then adding EDTA into the carbopol mixture, resulting in a water phase. To the oil phase add PG, surface active agents, emulsifiers and benzyl alcohol. Mix the oily phase with slow addition intothe water phase with continuous stirring at around 800-1200 RPM and blending the mixture for 15 to 20 minutes, resulting in an emulsion. To the emulsion, add triethanolamine to neutralizing the solution and formation of oleo gel and maintaining the pH of the composition in the range of 4 to 6. Further the composition is stabilized at room temperature for 6-10 hrs.
The main advantage of the present invention is that the present invention developsan anti-microbial composition which provides protection up to 6 hours of application..
Yet another advantage of the present invention is that the present invention providessanitization of facial skin by protecting from common pathogenic microbes generally present on facial skin area.
Yet another advantage of the present invention is that the present inventionprovides skin lightening and skin toning effects.
Yet another advantage of the present invention is that the present invention provides soothing and calming effect.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example.
DETAILED DESCRIPTION OF THE INVENTION
Definition
The terms “a” or “an”, as used herein, are defined as one or as more than one. The term “plurality”, as used herein, is defined as two as or more than two. The term “another”, as used herein, is defined as at least a second or more. The terms “including” and/or “having”, as used herein, are defined as comprising (i.e., open language). The term “coupled”, as used herein, is defined as connected, although not necessarily directly, and not necessarily mechanically.
The term “comprising” is not intended to limit inventions to only claiming the present invention with such comprising language. Any invention using the term comprising could be separated into one or more claims using “consisting” or “consisting of” claim language and is so intended. The term “comprising” is used interchangeably used by the terms “having” or “containing”.
Reference throughout this document to “one embodiment”, “certain embodiments”, “an embodiment”, “another embodiment”, and “yet another embodiment” or similar terms means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of such phrases or in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics are combined in any suitable manner in one or more embodiments without limitation.
The term “or” as used herein is to be interpreted as an inclusive or meaning any one or any combination. Therefore, “A, B or C” means any of the following: “A; B; C; A and B; A and C; B and C; A, B and C”. An exception to this definition will occur only when a combination of elements, functions, steps or acts are in some way inherently mutually exclusive.
As used herein, the term "one or more" generally refers to, but not limited to, singular as well as the plural form of the term.
The drawings featured in the figures are to illustrate certain convenient embodiments of the present invention and are not to be considered as a limitation to that. Term "means" preceding a present participle of an operation indicates a desired function for which there is one or more embodiments, i.e., one or more methods, devices, or apparatuses for achieving the desired function and that one skilled in the art could select from these or their equivalent in view of the disclosure herein and use of the term "means" is not intended to be limiting.
The present invention discloses an anti-microbial composition emulsion comprising of a first mixture and a second mixture. The first mixture comprises a Tea Tree oil in the range of 0.5 to 1.5%, a Rose oil in the range of 0.5 to 1.5%, a Eucalyptus oil in the range of 0.1 to 1.0%, a Turmeric Oil in the range of 0.1 to 1.0%, and a Licoriceoil in the range of 0.1 to 1.0%, resulting in an oil blend mixture. The second mixture comprises ofa Carbopol 940 in the range of 0.1 to 0.5%, EDTA in the range of 0.01 to 0.05g, and purifiedwater. Herein, the mixtures are obtained using a 3 blade marine impeller stirrer form a vortex having RPM in the range of 800-1200. In anembodiment the second mixture in the composition is a water phase. The first mixture in the compositionis anoil phase comprising all the essential oil blends in the range of 1 to 6% v/v.
In the preferred embodiment, the composition is prepared through the process comprising of stirring the weighed quality of carbopol 940 for 30-60 minutes at 800-1200 RPM in a vessel containing water then adding EDTA into the carbopol mixture, resulting in a water phase. To the oil phase add PG, surface active agents, emulsifiers and benzyl alcohol. Mix the oily phasewith slow addition into the water phase with continuous stirring at around 800-1200 RPM and blending the mixture for 15 to 20 minutes, resulting in an emulsion. To the emulsion, add triethanolamine to neutralizing the solution and formation of oleo gel and maintaining the pH of the composition in the range of 4 to 6. Further the composition is stabilized at room temperature for 6-10 hrs.
In anembodiment, the surface active agents includes,but not limited to, stearic acid, acetyl alcohol, PEG-100, stearate and glyceryl stearate, cetearylglucoside, polysorbate 20, polysorbate 80 methylcellulose, sodium carboxymethylcellulose, glycerine, bentonite, ceteareth-20, lecithin, hydrogenated lecithin, steareth-2, steareth-20, and polyglyceryl-2 stearate. In the preferred embodiment of the present invention, the surface active agent is polysorbate 80 in the composition.
In an embodiment,the emulsifiers includes,but not limited to,glycerine, polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, polyoxyethylene, polyoxypropylene glycol, polyethylene glycol, fatty acid polyethylene glycol, fatty acid polyoxy, polypropylene, sodium laurethsulfates, and specific preservatives, fragrances, and surfactants. In the preferred embodiment ofthe present invention, PEG400 is use as emulsifier in the composition
The following formulation was prepared and tested for rapid anti-microbial activity using Salmonella abony, Escherchia Coli, Psedumonasaeruginosa, Staphyococcusaureus and Candia albicans as test organisms. The results are shown in the table 1, showing broad spectrum antimicrobial activity (99.9%) with all the above pathogens. The tests are done using Antimicrobial susceptibility testing (ASM). They are used to discover foundational information on antibiotic classes, their modes of action, spectrum of activity, breakpoint determination and interpretation.

Table1.
TEST FOR ANTIMICROBIAL ACTIVITY
Testing Strain Antimicrobial Activity
Direct Application Dilution with DMSO
(1:1 Ratio) Zone of Inhibition(mm)
S,aureus No. Growth/Zone of Inhibition Observed No. Growth/Zone of Inhibition Observed 20.0mm
P.aeruginosa No. Growth/Zone of Inhibition Observed No. Growth/Zone of Inhibition Observed 22.0mm
Esch.Coli No. Growth/Zone of Inhibition Observed No. Growth/Zone of Inhibition Observed 20.0mm
Salmonella spp No. Growth/Zone of Inhibition Observed No. Growth/Zone of Inhibition Observed 21.0mm
Candida albicans No. Growth/Zone of Inhibition Observed No. Growth/Zone of Inhibition Observed 19.0mm

The following formulation was tested for rapid anti-microbial activity by direct application of the formulation on four different areas on one square centime area of each place. The test is conducted by ISO 18593 method. The tested result samples showed antimicrobial activity (99.9%) upto 6hours in case of an adult.

Volunteer 1 Cfu/cm2
Initial 1st Hour 2nd Hour 3rd Hour 4th Hour 5th Hour 6thHour
Forehead 8 2 ND ND ND ND ND
Left Cheek 10 ND ND ND ND ND ND
Right Cheek 12 ND ND ND ND ND 2
Chin 7 ND ND ND ND ND ND
Neck Area 15 4 ND ND ND ND 3

Volunteer 2 Cfu/cm2
Initial 1st Hour 2nd Hour 3rd Hour 4th Hour 5th Hour 6th Hour
Forehead 10 3 ND ND ND ND 4
Left Cheek 5 ND ND ND ND ND ND
Right Cheek 9 3 ND ND ND ND ND
Chin 7 ND ND ND ND ND ND
Neck Area 9 ND ND ND ND ND 3

Volunteer 3 Cfu/cm2
Initial 1st Hour 2nd Hour 3rd Hour 4th Hour 5th Hour 6th Hour
Forehead 5 ND ND ND ND ND ND
Left Cheek 8 ND ND ND ND ND ND
Right Cheek 3 ND ND ND ND ND ND
Chin 2 ND ND ND ND ND ND
Neck Area 6 ND ND ND ND ND 5

Volunteer 4 Cfu/cm2
Initial 1st Hour 2nd Hour 3rd Hour 4th Hour 5th Hour 6th Hour
Forehead 7 ND ND ND ND ND ND
Left Cheek 3 ND ND ND ND ND ND
Right Cheek 4 ND ND ND ND ND ND
Chin 4 ND ND ND ND ND ND
Neck Area 9 ND ND ND ND ND ND
*ND: Not Detected

Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided hereinbelow, in which various embodiments of the disclosed present invention are illustrated by way of example and appropriate reference to accompanying drawings. Those skilled in the art to which the present invention pertains may make modifications resulting in other embodiments employing principles of the present invention without departing from its spirit or characteristics, particularly upon considering the foregoing teachings. Accordingly, the described embodiments are to be considered in all respects only as illustrative, and not restrictive, and the scope of the present invention is, therefore, indicated by the appended claims rather than by the foregoing description or drawings. Consequently, while the present invention has been described with reference to particular embodiments, modifications of structure, sequence, materials and the like apparent to those skilled in the art still fall within the scope of the invention as claimed by the applicant.