DESC:Field of the Invention:
The present invention relates to the field of blood biochemistry. More particularly the present invention also relates to field of non-invasive blood biochemistry diagnosis of coronavirus infection in asymptomatic carriers.

Even more particularly the present invention relates to field of pathophysiology and psychophysiology diagnostic components wherein, it analyses the change in the body organ system wherein it provides an efficient system and process for non-invasive early stage detection of Coronavirus Infection and monitoring methods.

Background of the invention:
Modern diagnosis has become limited to Physiology Pathology where doctors diagnose a medical condition based on physical examination and pathological tests
like blood biochemistry tests. The problem with this approach is that most of the
diseases cannot be detected at the functional or asymptomatic stage where you
appear almost normal.
Detecting diseases at such an early stage can not only result in better treatment but also complete recovery. As psychophysiology and pathophysiology devices and tests are costly and are not easily available, these two components are completely
removed from the diagnosis. This has made it difficult for clinicians to effectively
detect the diseases at the early stage. There is no continues monitoring solution that enables the doctors and their patients to track the improvement with the treatment.
Therefore, there is no real-time feedback to the doctors on the improvement front.
If there was a comprehensive system that could allow the patients to monitor their
health on a regular basis during intervention and recovery period then any degradation in the health condition of the patients over a period of time could also
be informed to the doctor, leading him to review the intervention process.
It is being proved through past studies that pulse acquired from mobile camera can be used efficiently for comprehensive risk analysis for cardiovascular diseases.
Some earlier techniques present methods for non-invasive measurement of haemoglobin levels by continues analysis of the RGB histogram from mobile phone
images of the fingertip. These studies have progressed the non-invasive blood biochemistry diagnosis field. However, blood-biochemistry is affected by significant other aspects and they effect cardiovascular health in deterministic ways. As such, if cardiovascular health and non-invasive capillary analysis is correlated, then more accurate blood biochemistry indication can be obtained.

The moment a virus gets into a host body, the six different immune systems are
triggered to try to defend against the foreign body. Even before the first asymptomatic traits starts, the cytokinesis mechanism will be triggered. This mechanism is also overseen by the sympathetic branch of the Autonomic system
whereas the parasympathetic branch is responsible for managing the other organs. Not only does Heart Rate Variability can help profile early immune response of the body against the Virus, but at the same time the mechanism can be used to monitor the progression of different respiratory diseases as the respiratory system is directly modulated by the sympathetic branch of the autonomic Nervous system Kanva et. al. has proven that it is possible to capture Spo2 (peripheral capillary oxygen saturation) from the mobile camera accurately even for the Hypoxia patients and monitoring Spo2 is one of the major parts of the Corona Virus Decision Maker as suggested by WHO. Tian has presented in their study the role of pulmonary system in monitoring the pathogenesis as well as the pathological progression of the disease.

The effect of the virus in different groups have been proven to have different mortalities and severity. For example, Fang, Lei has explained the severity of the disease is more for diabetic and the hypertensive patients because, Human pathogenic coronaviruses (severe acute respiratory syndrome coronavirus [SARS CoV] and SARS-CoV-2) bind to their target cells through angiotensin-converting
enzyme 2 (ACE2), which is expressed by epithelial cells of the lung, intestine, kidney, and blood vessels. The expression of ACE2 is substantially increased in patients with type 1 or type 2 diabetes, who are treated with ACE inhibitors and angiotensin II type-I receptor blockers (ARBs). Hypertension is also treated with ACE inhibitors and ARBs, which results in an upregulation of ACE2. ACE2 can also be increased by thiazolidinediones and ibuprofen.
Therefore, monitoring the risk profile of the infected person is extremely important
for diabetic and hypertensive patients.

Reference is made to US20150182132A1, titled Mobile device system for measurement of cardiovascular health by applicant- CNV SYSTEMS Ltd. The invention provides a system that continuously monitors cardiovascular health using anelectrocardiography (ECG) source synchronized to an optical (PPG) source, without requiring invasive techniques or ongoing, large-scale external scanning procedures. The system includes an ECG signal source with electrodes contacting the skin, which generates a first set of information, and a mobile device having a camera which acts as a PPG signal source that generates a second set of information. Together with the mobile device's processor, configured to receive and process the first and second sets of information, from which the time differential of the heart beat pulmonary pressure wave can be calculated, continuous data related to cardiovascular health markers such as arterial stiffness can be determined. Variations of the ECG source may include a chest strap, a plug-in adaptor for the mobile device, or electrodes built into the mobile device.

Another reference is made to US20130072145A1, titled 911 SERVICES AND VITAL SIGN MEASUREMENT UTILIZING MOBILE PHONE SENSORS AND APPLICATIONS by applicant- University of North Texas. The invention provides Improved methods for utilizing 911 services, for implementing 911 dispatch protocols, and for measuring vital signs of a human, all by accessing mobile phone sensors and applications, are disclosed. Vital signs such as heart rate, breathing rate, breathing distress, and blood pressure can be measured using mobile phone sensors and applications. A method for differential estimation of blood pressure involves the synchronization of time between two mobile phones, locating an appropriate position for one cell phone and recording heart sounds, and recording video data from the finger tip of the subject using the other mobile phone.

Another reference is made to US20100268094A1, titled Consumer electronic
camera photoplethysmograph by applicant- Ocean it Laboratories Inc. The invention provides a body part is placed on or near a consumer electronic device camera. The camera senses periodic light variations. A program analyses variation in images captured by the digital camera to determine pulse rate or breathing rate.
Another reference is made to WO2020006518A1, titled Mobile device applications to measure blood pressure by applicant- Board Of Trustees Of Michigan State University. The present invention provides various systems, techniques, and embodiments are disclosed for implementing a blood- pressure measurement method that does not require specialized equipment (such as inflatable blood pressure cuffs).
The measurement can be taken from arterial locations within a user's finger, via the standard equipment and features of many widely-available consumer mobile devices. Such devices can be programmed to accurately and easily guide a user to press her finger on the screen of a device at a precise location, so that an accurate measurement can be taken. For example, guidance visualizations on a screen (such as finger silhouettes and animations) can be employed on the same screen on which a user presses her finger. Pressure sensitivity and optical camera readings are then used to calculate blood pressure for the user.

Another reference is made to KR20180053746A, titled System and method for obtaining vital measurements using a mobile device by applicant- Qualcomm Inc. The present invention provides Methods, systems, computer-readable media and apparatus for obtaining vital measurements are presented. Vital measurements may include blood pressure values that can be obtained by determining a pulse-transit time (PTT) as a function of a photoplethysmography (PPG) measurement and an ECG (electrocardiogram) measurement. The mobile device includes a user-portable outer body, a processor contained within the outer body, a display coupled to the light guide, and at least one first sensor coupled to the light guide. The display is configured to display an illumination pattern that directs light towards the blood vessels within the user. Wherein the at least one first sensor is configured to measure reflected light reflected from blood vessels within the user from the illumination m pattern and the processor obtains a first measurement indicative of changes in blood volume based at least in part on the measured reflected light.

Another reference is made to TWM542444U titled Wearable pulse contour analysis device with multiple diagnostic functions dated 2016-11-08 filed by Huang Shengrong. The present invention relates to a multiple function wearable device can analyze arterial pulse contour and can perform diagnosis. The essential component is an "arterial scan module" which contains LED as light source, photo diode as detector, and an MCU. With the innovated data procession algorithm and hardware design, more than 27 invaluable physiologic indexes can be extracted from enhanced high-resolution PPG signals collected by this device. Based on these results, further diagnosis about arterial stiffness, constriction power of ventricles, remaining blood volume, overall cardiovascular health, arteriosclerosis progress, SpO2, mental stress, autonomic nervous system activity measurements, and many more medical grade heart rate variability indexes. Blood pressure, respiration rate, and pulse frequency analysis can also be measured simultaneously. Even better, the same innovated device can measure the heart rates for both of pregnant woman and her fetus. The arterial scan module can be implemented into various products such as watch, bracelet, senior care devices, and smart clothing. This invention enables users to obtain all of these health-related indexes whenever they need and to receive notifications from any detected abnormal sign automatically.

Another reference is made to DE102006055691A1 titled Measuring device for non-invasive determination of physiological parameter, has diagnostic measuring unit integrated in keyboard of computer, and evaluating unit for determining physiological parameter by processing measuring signals dated 2006-11-23 filed by Flore Ingo Dr, Flore Ingo. The present invention relates to a measuring device for the non-invasive measurement of physiological parameters, comprising at least one diagnostic measuring unit for generating measuring signals and having an evaluation unit (140) for determining the at least one physiological parameter by processing the measuring signals. The object of the invention is to provide such a measuring device with extended functionality. For this purpose, the invention proposes that the at least one measuring unit is integrated in the keyboard of a computer.

However, none of the above discussed inventions provides a non-invasive and comprehensive around the clock detection of Coronavirus Infection by capturing arterial pulse from finger capillaries and by using light reflection processing from smart phone camera to find major part of Coronavirus decision marker known as “Peripheral Capillary Oxygen Saturation (spO2)” even from hypoxia patients. The present invention also utilizes “Cardiovascular Autonomic Neuropathy” (CAN) protocol along with the principle of “Photoplethysmography” (PPG), science of “Diabetic Cardiovascular Autonomic Neuropathy” and science of “Heart Rate Variability (HRV)” and glucose metabolism to estimate overall health status of people.

Objectives of the invention:
The principle objective of the invention is to provide round the clock available mobile tool which can be used by mass population as well as the authorities to effectively detect, asses risk, intervene, and manage corona virus using a non-invasive point-of-care.

Another objective of the present invention is to play a significant role in quickly detecting an accurate profile for Corona Virus Infection.

Another objective of the invention is to establish multi-parameter, multi-method correlated Profile (Rate of Change of Set of Biomarkers for different disease groups and/or different disease stage) and use it to detect and monitor coronavirus in the population.

Another objective of the invention is to provide instant non-invasive health assessment tool that offers pathophysiology and psychophysiology diagnostic components.

Another objective of the invention is to make it easier for clinicians to detect the
diseases at the early stage and help the patients to monitor their progress.

Another objective of the invention is to capture arterial pulse from finger capillaries by using light reflection processing from a smartphone camera, extracts arterial pulse using the principle of photoplethysmography (PPG) and then use Cardiovascular Autonomic Neuropathy (CAN) protocol for providing Psychophysiology and Physiopathology.

Another objective of the invention is to provide various functional biomarkers correlated to different early stage diseases which are derived from proven clinical tests and observations and thereafter combining the findings from all tests.

Another objective of the invention is to perform cardiovascular estimation from advanced second order PPG signal.
Another objective of the invention is to monitor progression of the disease.
Another objective of the invention is to identify and Classify the threat and risk profile of the patients.
Another objective of the invention is to analyse post-Quarantine monitoring and tracking.
Another objective of the present invention is to analyse to nervous system response, immune system response, metabolic response, respiratory response, circulatory response, endocrine response, bones and muscles response, vascular risk analysis (cumulative arterial risk, arterial impression, HRV analysis) due to cell hypoxia.
Another objective of the present invention is to analyse response to respiratory system, arterial pulse analysis, HRV linear analysis due to respiratory hypoxia.

Another objective of the present invention is to analyse glucose metabolism.

Yet another objective of the invention is to provide non invasive technique for obtaining body organ system wide biomarkers which can analyse the change in body organ system such as Respiratory System (Hemodynamic, FEV1/FEV6, Viscosity, Spo2), Nervous System (Autonomic and Peripheral), Vascular System (Arterial Stiffness and Vascular Elasticity), Functional Blood Biochemistry (Anaemic Profile, Lipids, Protein, Solute, Enzymes), Immunity System (Lymphocytes, Total Bilirubin), Endocrine System (Cardiac Rhythm), Lifestyle (Sleep, Energy, Cardiac Fitness, body pain, metabolism), Psychological Health (Depression, Anxiety, Chronic and Acute Stress).

Summary of the invention:
The present invention provides a non-invasive, smart phone-based tool towards
comprehensive early stage detection of Coronavirus Infection by capturing arterial pulse from finger capillaries and by using light reflection processing from smart phone camera to find major part of Coronavirus decision marker known as “Peripheral Capillary Oxygen Saturation (spO2)” even from hypoxia patients, uses Cardiovascular Autonomic Neuropathy (CAN) protocol for providing Psychophysiology and Physiopathology.
The present invention makes it easier for clinicians to detect the diseases at the early stage and helps the patients to monitor their progress. It offers a set of various functional biomarkers, which are correlated to various asymptomatic conditions related to various diseases.
The system analyses photo reflection at the blood capillary in the finger using the principle of photoplethysmography (PPG) and then uses a proven science of Diabetic Cardiovascular Autonomic Neuropathy to estimate the overall health status through a set of easy, static and dynamic tests and proven vitals. The present invention performs cardiovascular estimation from advanced second order PPG signal whereas the estimation of the state of Autonomic Nervous system is performed using proven science of Heart Rate Variability (HRV).
The present invention provides non-invasive indication of Haemoglobin, TSH, Protein levels and Uric Acid and Immunity components like platelet, bilirubin, WBC count and lymphocytes.

Statement of the invention:
An efficient system and process for non-invasive early stage detection of Coronavirus Infection and monitoring thereof wherein the invention comprises of mobile based tool, the said system capture arterial pulse from finger capillaries by using light reflection processing from a smartphone camera, extracts arterial pulse using the principle of photoplethysmography (PPG), use Cardiovascular Autonomic Neuropathy (CAN) protocol for providing Psychophysiology and Physiopathology. The present invention provides system and process to monitor the progression of disease, to analyse response to respiratory system, arterial pulse analysis, HRV linear analysis due to respiratory hypoxia and cell hypoxia, the said analysis of glucose metabolism, the said system and process provides functional biomarkers, and analysis of asymptomatic patients.

Brief Description of Drawings:
Figure 1, represent hypothesis of the Coronavirus infection case use for the analysis of body system wherein to the left of the parameter (9) the study and analysis to obtain profile of virus/disease in relation to rate of change of these parameters is done and to the right of parameters (9) it mentioned that individual can perform test on phone and see how they compare against developed profile for risk analysis.
Figure 2, represent acquiring Pulse Signal from smartphone camera for the test.
Figure 3, represents overview of the objective involved in the present invention wherein it begins with study the existing patients (22), understand change in various parameters and develop disease profile (23), symptomatic people monitor those parameters from their homes on their phone (24), individual’s risk is analysed based on developed disease profile (25) and appropriate action is referred based on risk (26).
Figure 4, represent conceptual System-wide diagram of calculating functional Biomarkers wherein it shows cardio regulatory centres in medulla oblongata (27), parasympathetic nerve fibre (28), sympathetic nerve fibre (29), bioreceptors in the wall of carotid artery (30), carotid sinus nerve to nerve IX (31), vagus nerve X (32), bioreceptors in aorta (33), erratic rhythm (34), heart (35), circulation (36), dynamic vascular score (37), sympathetic dominance (38), left ventricular after load (39), parasympathetic impairments (40), heart rate and arrhythmia (41).
Figure 5, represents the principles of photoplethysmography in the mobile application of the present invention wherein it shows conventional pulse oximeter which works by measuring blood volume change which is detected through light refraction by blood uses IR and red LEDS (42), use of camera flash light to glow fingertip (43), capturing continuous frame of fingertip (44), finally gives report (47), blowing mike like expulsing a balloon (45), exercise (46), signalling process (53), data backup (52), propitiatory signal processing and extract an ECG signal called advanced PPG (50), processing signal live (51), change of intensity due to blood volume change as signal (48) and spectral data extraction (49).

Figure 6, represents another result of cell hypoxia wherein ACE2 receptor expression which is managed by the Sympathetic branch of the Autonomic system and therefore there is a severe stress in that branch.

Figure 7, represents another result in cell hypoxia analysis wherein Overall Metabolic stress is increased and metabolic function is reduced due to Oxidative stress. This is due to increase of free radicals in Hypoxia which temporarily increases the Lactic acid. Acidosis results in reduction of the Metabolic function. As ACE2 is expressed by various parts of the GI track, a frequent change in this can mean Metabolic stress. Prolonged Hypoxia further increases the respiratory rate.
Figure 8, represents another result in cell hypoxia analysis wherein, due to HPA axis signalling and increase in the Sympathetic stress, results in increase of the Cortisol, Endocrine system performance degrades, bone and muscle strength degrades, and circulatory system performance degrades (due to falling Hemodynamic, AF).
Figure 9, represents spectral vibration during cell hypoxia analysis wherein it shows baseline normal (54), mild choking (55), moderate choking (56), severe choking (57).
Figure 10, represents vascular risk analysis wherein it shows cumulative arterial risk at baseline normal (58), mild choking (59), moderate choking (60), severe choking (61). In this analysis it is shown that Cell Hypoxia doesn’t causes Arterial Stiffness. As arterial stiffness is a unique characteristic observed in COVID due to cell rupture and involvement of Plamin. D-dimmer increase in the blood can be resulted in COVID involvement in the arterial stiffness. Normal Necrosis or Necrosis resulted from Acidosis can therefore be distinguished from COVID using arterial stiffness measurement.
Figure 11, represents arterial impressions wherein it shows baseline normal (62), mild choking (63), moderate choking (64), severe choking (65).
Figure 12, represents HRV linear analysis wherein it shows baseline normal (66), mild choking (67), moderate choking (68), severe choking (69) during cell hypoxia analysis.
Figure 13, represents summary by subject tests in varied breathing pattern shock to the system leading to Respiratory Hypoxia wherein baseline (70), data with running nose (71), controlled breathing rate (72). The Respiratory rate captured in the present invention by analysing the breathing pattern from Microphone. It can be seen that system can distinguish between deep breathing, regular breathing, breathing rate, breathing with running nose and so on. A Respiratory Hypoxia can be simulated by not breathing for a minute or by maintaining one breath par minute.
Figure 14, represents the modulation of the Arterial Pulse signal captured by the Arterial pulse. Therefore, the invention also provides the system wherein it can capture the respiratory quality of the body without the Microphones from the Arterial pulse separated from the capillary pulse. The graph shows baseline normal (73), breathing only twice (74), controlled breathing rate at 6 bpm (75), breathing only 6 times (76), with running nose (77).
Figure 15, represents HRV analysis of the subject test in varied breathing pattern shock to the system leading to respiratory Hypoxia.
Figure 16, represents cumulative arterial risk (83) and arterial impression (84) in base line normal, cumulative arterial risk (85) and arterial impressions (86) in controlled breathing rate 6 bpm, cumulative arterial risk (87) arterial impressions (88) in breathing only twice.
Figure 17, represents cumulative arterial risk (89) and arterial impression (90) when breathed only 3 times holding for 40 secs each, cumulative arterial risk (91), arterial impression (92) when inhaled a lot of water through nose before test analysis with running nose.
Figure 18, represents subject tests in Normal condition (93) V/s Consuming Sweet (94) during the test of glucose metabolism.
Figure 19, represent glucose metabolism affected component.

Detailed description of the invention:
It should be noted that the particular description with features, designs, components, construction, working and embodiments set forth in the specification below are merely exemplary of the wide variety and arrangement of instructions which can be employed with the present invention. The present invention maybe embodied in other specific forms without departing from the spirit or essential characteristics thereof. All the features disclosed in this specification may be replaced by similar other or alternative features performing similar or same or equivalent purposes. Thus, unless expressly stated otherwise, they all are within the scope of present invention. Various modifications or substitutions are also possible without departing from the scope or spirit of the present invention. Therefore, it is to be understood that this specification has been described by way of the most preferred embodiments and for the purposes of illustration and not limitation.
The present invention provides a non-invasive, smart phone-based tool towards
comprehensive and non-invasive early stage detection of Coronavirus Infection by
capturing arterial pulse from finger capillaries and by using light reflection processing from smart phone camera to find major part of Coronavirus decision marker known as “Peripheral Capillary Oxygen Saturation (spO2)” even from hypoxia patients.
The present invention makes it easier for clinicians to detect the diseases at the early stage and helps the patients to monitor their progress by offering a set of functional biomarkers, which are correlated to various asymptomatic conditions related to various diseases.
The present invention provides alternative screening tool to combat the challenges posed by the deployed tests based on two parameters such as Capturing marker specific prognostic tool to COVID-19 for identification of asymptomatic cases and a non-invasive tool, which is scalable, useful even at the remote point of care of setting and easy to handle without the requirement of exceptional skills.

The invention is based on the principles of photo-plethysmography (PPG) and photo chromatography (PCG). PPG measures blood volume changes in the microvascular and PCG measures the reflected light of various blood components. This is carried out from an optical sensor (camera and the flashlight of a smartphone) acting as an input layer. A signal processing layer, our proprietary mathematical modelling, and an algorithm convert the input signal into actionable metrics that constitute the functional biomarker parameters.
The principle is to provide non-invasive indication of Haemoglobin, TSH, Protein levels and Uric Acid and Immunity components like platelet, bilirubin, WBC count and lymphocytes.
The invention provides overall organ system health and a separate score for Diabetic Autonomic Neuropathy and the system can be used efficiently for cardiovascular early diagnosis using techniques mentioned in the invention.
With the help of the present invention a controlled clinical study was conducted on 25 (n=25) subjects to prove the hypothesis and establish the difference in between an infected and non-infected group of individuals. The study derived out of this clinical study was found to be different between the two groups. The positive infected test group had a significantly high (p=.00012) median score of 19.8(range 9.9 to 22.1) compared to 6.9(range 2.2 to 7.8) that of the healthy uninfected control group. The accuracy, sensitivity, and specificity of the study were found to be 92%, 93.2%, and 90% respectively. The result of this study is, therefore, evidence to use the present invention as a potential tool in the identification, screening, and surveillance of asymptomatic individuals.

The present invention provides a way and means to identify, differentiate, and prioritize confirmatory testing for asymptomatic but infected individuals. This strategy certainly helps in community-level screening and surveillance of the infection with no risk of invasive procedures. On one hand, it will help us to identify high-risk asymptomatic individuals, on the other hand, reduce the burden on subjecting healthy uninfected individuals to the expensive confirmatory RTPCR testing in an already resource constraint setting.
The Non-invasive technique for obtaining body organ-system wide biomarkers which can analyse the change changes in the body organ systems such as: Respiratory System (Hemodynamic, FEV1/FEV6, Viscosity, Spo2), Nervous System (Autonomic and Peripheral), Vascular System (Arterial Stiffness and Vascular Elasticity), Functional Blood Biochemistry (Anaemic Profile, Lipids, Protein, Solute, Enzymes), Immunity System (Lymphocytes, Total Bilirubin), Endocrine System (Cardiac Rhythm), Lifestyle (Sleep, Energy, Cardiac Fitness, body pain, metabolism), Psychological Health (Depression, Anxiety, Chronic and Acute Stress).
The functional biomarkers as shown in Table-1 below in more detail
Autonomic Nervous System Autonomic Neuropathy Autonomic Nervous System Impairment, Autonomic psycho-physiological stress.
Autonomic Homeostasis Autonomic imbalance, Sympathetic, Parasympathetic score.
Vascular Endothelium Dysfunction, Non-invasive Left ventricular afterload quality, Atrial Stiffness,
Cardio Fitness Vo2Max Exercise, Vo2Max Normal.
Rhythm Ectopic, Ischemic, Erratic Rhythm
Sinus and Non-Sinus Arrhythmia, Heart/Pulse rate.
Autonomic Cardiac Neuropathy Autonomic Cardiac impairment, Autonomic Cardiac Power
ECG
Electrophysiology AF, MI, VT detection, ST, QTc, PR, QT dispersion, Axis
Pulmonary FEV1/FEV6, Respiratory Rate, Spo2, Viscosity
Endocrine Erratic Rhythm, Vascular elasticity, Hemodynamic Quality, Non-Invasive CBC Quality
Metabolism Non-invasive glucose metabolism impairments, Autonomic metabolic impediments, Non-invasive blood solute property.
Immunity Presence and level of active immune dynamics (for infection, inflation, tumors)
Physiology Appetite, Digestion, Sleep, Body Pain, Stress, Low Work Energy

Bone and Muscles Gripper Strength Test
Tests HRV, 1-12 Lead Concurrent ECG, Spirometry, Orthostatic Homeostatic, Pulse Rate Variability, Exercise Vo2Max, Gripper Strength Test, Ishihara Color Blindness based Retinopathy (under trial), Hearing Loss Test Audiometry (Under trial)

By capturing capillary pulse from the finger capillary using mobile camera (as shown in figure 2), respiratory envelop by using mobile microphone, ambient light from the ambient light sensor and tremor analysis signal by accelerometer sensor of the phone using set of static tests (Sitting HRV) and dynamic tests (Orthostatic, Gripper, Suboptimal Vo2Max Stair test and Spirometry tests) are also performed.
In this way the present invention helps in detection of Corona Virus infection at an early stage, monitor progression of the disease, identify and Classify the threat and risk profile of the patients, analyse the immune system response of the body, post-Quarantine monitoring and tracking.
The objective is to achieve the analysis in two stage, First, ground truth data collection phase where data from subjects of the known conditions is collected and distinct profiles is created and in second phase, the data from test subjects is collected and classified using developed profiles. Accuracy of the classification would be calculated using blind review method. Further, sensitivity and specificity of the detection and progress monitoring accuracy would be calculated.
Disease progression profile for coronavirus is created by studying positive patient data to develop the algorithm for a non-invasive, smartphone-based screening and health monitoring test for the mass population wherein existing patients were studied to understand change in various parameters and development of disease profile. The symptomatic people were monitored from their home. The individual risks were analysed based on developed disease profile and thereafter appropriate action were taken based on risk assessment.

The technology performs cardiovascular estimation from advanced second order PPG signal whereas the estimation of the state of Autonomic Nervous system is performed using proven science of Heart Rate Variability.
The Presents methods for non-invasive measurement of haemoglobin levels by continues analysis of the RGB histogram as shown in Figure 9 from mobile phone images of the fingertip. These studies have progressed the non-invasive blood biochemistry diagnosis field. However, blood-biochemistry is affected by significant other aspects and they effect cardiovascular health in deterministic ways. As such, if cardiovascular health and non-invasive capillary analysis is correlated, then more accurate blood biochemistry indication can be obtained.

As the core of the present invention is heart rate variability wherein it helps in earliest profile detection of the virus entry through a HRV profile itself. At same time the mechanism can be used to monitor the progression of different respiratory diseases as the respiratory system is directly modulated by the sympathetic branch of the autonomic Nervous system as well.
The present invention helps in quickly detecting an accurate profile for Corona Virus Infection, it’s effect and spread and the profile can then be used by a mass population as well as the authorities to effectively detect, asses risk, intervene, and manage corona virus using a non-invasive point-of-care round the clock available mobile tool.
The present invention provides solution of asymptomatic disease diagnosis by providing various functional biomarkers correlated to different early stage diseases which are derived from proven clinical tests and observations such as Pulse Morphology, Rhythmic Abnormality, Autonomic Neuropathy, Blood Biochemistry, Heart Rate Variability, Exercise Vo2Max test, Stress Analysis, Endothelial Quality check and thereafter combining the findings from all tests.
The present invention consisting of smartphone based technology wherein its usage comprises of step such as first-input basic parameters and any symptoms, family history and personal medical history , second-selection of the test to be performed default is 2 mins HRV and orthostatic tests, third- user sits calmy places fingertip on rear camera and follows audio instructions from the application, fourth, a detailed report generated instantly with preliminary diagnosis ready to be shared with clinicians finally history of the patient can be tracked over time to see progress.
Various functional biomarker such as Cell Hypoxia, Pulmonary/Respiratory Hypoxia, Glucose Metabolism were analysed with help of the present invention. In case of cell hypoxia Tissue Necrosis which was simulated by Choking the Venus blood supply with rubber band. Tissue Necrosis also causes oxidative stress in the body. As the stress is managed by the autonomic branch of the Nervous system, the nervous system performance comes down. ACE2 receptor expression is managed by the Sympathetic branch of the Autonomic system and therefore there is a severe stress in that branch. However, this state doesn’t cause any immune response. So, the Hypoxia in any part of the body can be captured in isolation to the immune response as shown in figure 6. Further, Overall Metabolic stress is increased and Metabolic function is reduced due to Oxidative stress. This is due to increase of free radicals in Hypoxia which temporarily increases the Lactic acid. Acidosis results in reduction of the Metabolic function. As ACE2 is expressed by various parts of the GI track, a frequent change in this can mean Metabolic stress as shown in figure 7. Further, Due to HPA axis signalling and increase in the Sympathetic stress, resulting in the increase of the Cortisol, Endocrine system performance degrades, bone and muscle strength degrades, and circulatory system performance also degrades (due to falling Hemodynamic, AF) as shown in figure 8. Further vascular risk analysis is cumulative arterial risk analysis arterial stiffness is a unique characteristic observed in COVID due to cell rupture and involvement of Plamin. D-Dimmer increase in the blood can be resulted in COVID-19 involvement in the Arterial stiffness. Normal Necrosis or Necrosis resulted from Acidosis can therefore be distinguished from COVID using Arterial Stiffness measurement. and heart rate analysis was done as shown in figure 10, arterial impression analysis as shown in figure 11 and figure 12.
In respiratory hypoxia, as shown in figure 13, figure 14, figure 15, and figure 16, figure 17 it can be observed through arterial impression graph. This happens because when there is Hypoxia, it induced Sympathetic dominance which further increases the ACE2 to ACE1 conversion that results in vasoconstriction or shortening of the thickness of the Arteries. Such a stiffness is also called Physiological Arterial stiffness. A physiological Arterial Stiffness is a function of Flight and Fight response of the body. In order to isolate the Physiological Arterial Stiffness from the Pathological Stiffness (Atherosclerosis resulting from the Diabetic induced calcification), the spectrum was observed. In the presence of a vascular occlusion, we can analyse that there is a probable Atherosclerosis. In case of COVID, an Arterial Stiffness change can reflect external body-based changes. Though Acidosis can also cause Physiological Arterial Stiffness, a change in the Protein value (resulting from the Metabolic stress) can therefore be used to separate the Acidosis stress to COVID induced Arterial stiffness.
In glucose metabolic analysis as shown in figure 18 and figure 19, as the external envelop of COVID has Glycoprotein layers, in the case of Viral Shedding, the glucose in the blood increases Also in the case of cell rapturing, glucose energy is released in the cell. This is simulated by externally shooting the glucose levels by consuming 3TSP of Honey. The hypothesis is that in the case of absence of external sugar consumption, if test is taken before food, change in the parameters due to increase in the sugar in the blood can reflect the possible presence of a viral attack.

The accuracy summary obtained in the present invention after analysis of various test are mentioned in Table-2 below:
Particulars %
Asymptomatic COVID Detection Accuracy 92%(Phase 1), 95%(Phase 2)
Sensitivity 93%(Phase 1), 100%(Phase 2)
Specificity 90%(Phase 1), 80%(Phase 2)

In an exemplary embodiment, the said system provides the non-invasive diagnostic solution capture arterial pulse from finger capillaries by using light reflection processing from a smartphone camera.
In another exemplary embodiment, the said system helps in extraction of arterial pulse using the principle of photoplethysmography (PPG) and then use Cardiovascular Autonomic Neuropathy (CAN) protocol for providing Psychophysiology and Physiopathology.
In another exemplary embodiment, the said system helps in establishing multi-parameter, multi-method correlated Profile (Rate of Change of Set of Biomarkers for different disease groups and/or different disease stage) and use it to quickly to detect and monitor coronavirus in the population.
In another exemplary embodiment, the said system helps in the diagnostic analysis due to cell hypoxia, respiratory hypoxia and glucose metabolism.

In another exemplary embodiment, the said system involves the functional biomarkers correlated to different early stage diseases which are derived from proven clinical tests and observations such as Pulse Morphology, Rhythmic Abnormality, Autonomic Neuropathy, Blood Biochemistry, Heart Rate Variability, Exercise Vo2Max test, Stress Analysis, Endothelial Quality check and thereafter combining the findings from all tests.
In another exemplary embodiment, the said system involves check analysis of asymptomatic abnormalities and enable speedy point of care diagnosis.

In another exemplary embodiment, the objective is to be achieved in two stages, the ground-truth data collection phase where data from subjects of the known conditions will be collected and distinct profiles will be created. In the second phase the data from test subjects will be collected and classified using developed profiles.
In another exemplary embodiment the accuracy of the classification would be calculated using blind review method. Further, sensitivity and specificity of the detection and progress monitoring accuracy would be calculated.

Advantages of the invention:
1. The present invention helps in quickly detecting an accurate profile for Corona Virus Infection.
2. The present invention helps in providing round the clock available mobile tool which can be used by mass population as well as the authorities to effectively detect, asses risk, intervene, and manage corona virus using a non-invasive point-of-care.
3. The present invention helps in establishing multi-parameter, multi-method correlated Profile (Rate of Change of Set of Biomarkers for different disease groups and/or different disease stage) and use it to detect and monitor coronavirus in the population.
4. The present invention helps in providing instant non-invasive health assessment tool.
5. The present invention helps in using light reflection processing from a smartphone camera and then use Cardiovascular Autonomic Neuropathy (CAN) protocol for providing Psychophysiology and Physiopathology.
6. The present invention helps in providing various functional biomarkers correlated to different early stage diseases which are derived from proven clinical tests and observations such as Pulse Morphology, Rhythmic Abnormality, Autonomic Neuropathy, Blood Biochemistry, Heart Rate Variability, Exercise Vo2Max test, Stress Analysis, Endothelial Quality check and thereafter combining the findings from all tests.
7. The present invention also helps in providing non-invasive indication of Haemoglobin, TSH, Protein levels and Uric Acid and Immunity components like platelet, bilirubin, WBC count and lymphocytes.
8. The present invention also helps in providing a technique to find out SpO2 (peripheral capillary oxygen saturation) from the mobile camera accurately even for the Hypoxia patients since SpO2 is one of the major parts of the Corona Virus Decision Maker.
9. The present invention also helps in providing respiratory profile which not only attributes towards a comprehensive detection but also enables efficient monitoring of the patients.
10. The present invention also helps in providing the mechanism which can be used to monitor the progression of different respiratory diseases as the respiratory system is directly modulated by the sympathetic branch of the autonomic Nervous system.
11. The present invention aides in asymptomatic disease diagnosis.
12. The present invention is Instant, Portable, Accurate, Fast and Scalable.
13. The present invention provides accuracy of 90.72%.
14. The invention gives instant result.
15. Portable.
16. Faster analysis.
17. Scalable.
18. Needs no internet.
19. Extensive testing and validation.
20. Rigorous clinical validation.
,CLAIMS:We claim:
1. An efficient system and process for non-invasive early stage detection of Coronavirus Infection and monitoring thereof wherein the invention comprises of mobile based tool, the said system capture arterial pulse from finger capillaries by using light reflection processing from a smartphone camera (2), extracts arterial pulse using the principle of photoplethysmography (PPG), the said system and process uses Cardiovascular Autonomic Neuropathy (CAN) protocol for providing Psychophysiology and Physiopathology, the said process to monitor the progression of disease (22), the said system to analyse response to respiratory system, arterial pulse analysis, HRV linear analysis due to respiratory hypoxia and cell hypoxia, the said analysis of glucose metabolism , the said system and process provides functional biomarkers, the said system and process analyses of asymptomatic patients.
2. The system and process as claimed in claim 1, wherein the non-invasive diagnostic solution capture arterial pulse from finger capillaries by using light reflection processing from a smartphone camera.
3. The system and process as claimed in claim 1, wherein the said system extracts arterial pulse using the principle of photoplethysmography (PPG) and then use Cardiovascular Autonomic Neuropathy (CAN) protocol for providing Psychophysiology and Physiopathology.
4. The system and process as claimed in claim 1, wherein it helps in establishing multi-parameter, multi-method correlated Profile (Rate of Change of Set of Biomarkers for different disease groups and/or different disease stage) and use it to quickly to detect and monitor coronavirus in the population.
5. The system and process as claimed in claim 1, wherein the diagnostic consists of analysis due to cell hypoxia, respiratory hypoxia and glucose metabolism.
6. The system and process as claimed in claim 1, wherein the functional biomarkers correlated to different early stage diseases which are derived from proven clinical tests and observations such as Pulse Morphology, Rhythmic Abnormality, Autonomic Neuropathy, Blood Biochemistry, Heart Rate Variability, Exercise Vo2Max test, Stress Analysis, Endothelial Quality check and thereafter combining the findings from all tests.
7. The system and process as claimed in claim 1, wherein the overall efficiency of the system is 90.72%.
8. The system and process as claimed in claim 1, wherein the present invention provides check analysis of asymptomatic abnormalities and enable speedy point of care diagnosis.
9. The system and process as claimed in claim 1, wherein the analysis consists of steps such as:
I. The ground-truth data collection phase where data from subjects of the known conditions will be collected and distinct profiles will be created.
II. The data from test subjects will be collected and classified using developed profiles.
III. Accuracy of the classification would be calculated using blind review method.
IV. Further, sensitivity and specificity of the detection and progress monitoring accuracy would be calculated.