TITLE:
A herba! nutraceutical formulation for adolescent girls.
FIELD OF THE INVENTION:
This invention relates to a herbal nutraceutical formulation for adolescent
girls.
BACKGROUND OF THE INVENTION:
"Nutraceutical" is any substance that may be considered a food or part of
a food and provides medical or health benefits, encompassing prevention
and treatment of diseases. Such products may range from isolated
nutrients, dietary supplements and diets to genetically engineered
"designer" foods, herbal products and processed foods such as cereals,
soups and beverages. "Nutraceuticals and functional foods have received
considerable interest because of their presumed safety and potential
nutritional and therapeutic effects". There is, thus, a proliferation of these
value-added products aimed at not only keeping oneself healthy but also
prevention/treatment of various ailments ranging from heart diseases to
cancer. The main aim is based on the disease preventing phytonutrients
present in herbal plants. Phenols, which are a large group of
phytonutrients, have great importance in preventive medicine.
Phytochemical is used to enhance the efficacy of Vitamin C. tt can also
act against allergies, ulcers, tumors, platelet aggregation, controlling
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hypertension and reduces the risk of estrogen induced cancer. The food
plays significant role in the general health of women. The right food has
the power to heal, keeping good health of body functions, ageing,
rejuvenating, immuno-modulating and prevention against diseases.
Reference is made to Bhutani K K, Jadhav A N and Katia v (Effect of
SymplocosracemosaRoxb. on gonadotropin retease in immature femate
rats and ovarian histology." J Ethnopharmacot. 2004 s"p; 94 (1):1gl-
200); Gaitonde B and Jetmalani M H (Antioxytocic action of sapopin
isolated from Asparagus racemosusWilld (Shatavari) on uterine muscle.
Arch lnt Pharmacodyn Ther. 1969 M.y; 179 (1):121-9); Miranda, Maria
and Preethi (Effectiveness of selected home remedies in reducing the
pain and discomfort of dysmenorrheal among adolescent girls in a
selected high school at mangalore. Rajiv Gandhi University of Health
sciences, Karnataka, Bangalore, 2005); Mukund sabnis (2006 chemistry
and Pharmacology of Ayurvedic medicinalplants. Chaukhamba
Amarabharati publication, varanasi); Babita Gupta, Satish Nayak and
SaritaSolanki (Abromaaugusta Linn f: A review Der Pharmacia Sinica,
2011, 2(4):253-261); H. Sh, A. Hanif, B. Agarwal, R. Mohammed and J.
Rowank. A journal of plants, people and applied Research Ethnobotany
research and applications.2010, (8), 61 -74); Anonymus (The wealth of
lndia" dictionary of lndian raw materials and industrial products.The
council of scientific and industrial research. (NISCAIR press publisher;
New Delhi 2006, 222); Prajapati N D, Purohit S S, Sharma A K and
Kumar T (A hand book of medicinal plant, A complete source book.
(Agrobios (lndia) publisher; Jodhpur 2003, 2); Nandkarni K M. "lndian
material medica", vol.l, (Bombay popular prakashan; Mumbai,2OO2, 4);
-3-
Agro techniques of selected medicinal plants (vol.l, National medicinal
ptants board. Department of AYUSH, ministry of health and family
welfare, (TERI press The Energy and resources lnstitute. New Delhi,
2008, 1); [\ Rahamtullah, D Nasrin and Jahan I (Advance in natural and
applied science. 2010,4(2),99-10a); Mishra M, Ghosh G, Mishra D and
Barik B (Advance in pharmacology and toxicology. 2008, 9(3), 147-150);
Lee Do Y, Choo B K, Yoon T, Cheon M S, Lee H W, Lee A Y and Kim H
K (Anti-inflamatory effect of asparagus cochinchinesis extract in acute
and chronic cutaneous inflammation. Journal of Ethnopharmacology, 121
(1): 28-34, 2009); Kumar M C, Udupa A L, Sammodavardhana K,
Rathankar U P, Shvetha U and Kodancha G P (Acute toxicity and
diuretic studies of the root of Asparagus recemosus Wild in rats.West
lndian Medical Jouranal, 59 (1): 3-6, 2010); Santosh Kumar, R K Mehla
and A K Dang (Use of Shatavari (Asparagus Racemosus) As
Agalactopoietic and Therapeutic Herb- A ReviewAgric. Rev., 29 (2).132-
138, 2008); Nadkarni K M (lndian plants and drugs with their medicinal
properties and uses. Norton and Co. Madras: 1910. p. 120); Sharma P C,
Yelne M B and Dennis T J (Database on medicinal plants used in
Ayurveda.Vol. 1. New Delhi: lndia Central Council for Research in
Ayurveda and Siddha, 2000. Pp, 150; Sharma P C, Yelne M B and
Dennis T J (Database on medicinal plants used in Ayurveda.Vol. 1. New
Delhi: Central Council for Research in Ayurveda and Siddha, 2000. p.
152,154); Khare C P (lndian medicinal plants. New Delhi: Springer (lndia)
Pvt, Ltd., 20070): Menopause & the Role of Hormone Therapy in lndian
Women. The Third National Revised Consensus Meeting Guidelines of
lndian Menopause Society 2008 (A division of Reed Elsevier lndia Private
Limited. ISBN 978-81 -312-1577 -7);
4-
Komal sharma and Maheep Bhatnagar (Asparagus racemosus
(Shatavari): A Versatile Female Tonic. lnternational Journat of
Pharmaceutical & Biological Archives 2011; 2(3):855-863); sharma R K,
Dash B and CharakaSamhita - Text with English Transtation and Critical
Exposition Based on ChakrapaniDatta's Ayurveda Dipika. Chowkhamba;
varanasi, lndia; 2003); Garde G K and sarthvagbhat (Marathia
Translation of Vagbhat's Astangahridya); Uttarstana (Aryabhushana
Mudranalaya, pp. 40-48; 1970); Atreya (Ayurvedic Heating for women.
Samuel Weiser, lnc, York; 1999); Srikantha M and K R
vagbhata'sAstangaHrdayam - Text, English translation, Notes, Appendix
and lndices.Krishnadas Academy; Varanasi; 1997); Gaitonde B B,
Jetmalani M H (Antioxytocic action of saponin isolated from Asparagus
racemosusWilld (Shatavari) on uterine muscle. Arch. lnt. Pharmacodyn.
Ther. 1969; 179: 121-129); Hemadri K and Rao s s (Leucorrhoea and
menorrhagia: tribal medicine. Ancient Science of Life 1983; 3(1): aOal);
Pugalendhi V, Karunanithi R, Panicker T M R, Abraham C,
Gurumurthy P and Jagadeesan K A (Pilot study on the Breast
Development in Female Wistar Rats Using an lndigenous Herbat
preparation by topical application Bombay Hosp Jour 2004; 46: 03);
Tewari P V, Mata H C, chaturvedi c. Experimental study on estrogenic
activity of certain indigenous drugs. J. Res. lnd. Med. yoga and Homeo
1968; 11: 4-12.
tr
OBJECTS OF THE INVENTION:
An object of this invention is to propose a herbal nutraceuticat formulation
for adolescent girls.
Another object of this invention is to propose a combination of herbal
plants with a base material to provide essentiat nutrient.
Still another object of this invention is to develop a disease protective
nutraceutical formulation to improve the general health of adolescent girls
as food supplement.
BRIEF DESCRIPTION OF THE INVENTION:
This invention relates to a herba! nutraceutical formulation for adolescent
girls comprising: 20 - 40o/o Abroma augustus, 55-4Oo/o Sarraca indica, 15-
25o/o symplocos racemosa and 10-20% Asparagus officinalis.
ln accordance with this invention there is also provided a method for
preparing herbal neutraceutical formulation comprising: obtaining and
cleaning fresh matter roots of Abroma augustus, drying and pulverizing
the clean roots, subjecting the pulverized powder to the step of extraction
in hot water, drying the filtrate, obtaining and cleaning the mature bark of
the tree of Saracea indica, drying the cleaned bark, subjecting the dried
bark to the step of pulverization into form powder extracting them fine
powder with hot water and cotlecting the filtrate, preparing acetic acid
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solution from the extract and drying the same, obtaining and cleaning the
stem and roots of Asparagus officinalis drying and then pulverizing the
clean stem and roots, subjecting the powder to the step of extraction with
water drying the extract, collecting and cleaning the mature bark of
Symplocos racemosa, drying and then pulverizing the cleaned bark,
subjecting the powder form of the bark to the step of extraction with water,
collecting the extract fraction and drying the same, mixing all the extract in
the following fractions:
100-200 mg of Abroma augustus
300400 mg of Sarraca indica,
50-100 mg of Symplocos racemosa,
250-350 mg of Asparagus officinalis
DETAILED DESGRIPTION OF THE INVENTION:
The present invention relates the nutraceutical compositions in a proper
combination of natural medicinal plants/herbs to provide optimum nutrition
health protective, preventive and promotive benefits to improve the
general health of adolescent girls.
The given process defines extraction of the selected portion of herbs. The
nutraceutical contains specific extract fraction in definite proportion and
also contains some diluents and excipients. The details provided for
preparation of the following abstracts reflect the presently preferred
method for extract preparations and not be consider as limiting. The
quantities and times described below can be varied substantially to
provide suitable extracts in accordance with the invention.
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The process extraction involves the following steps of: obtaining fresh
mature roots of Abroma augustus and cleaning the obtained roots with
distilled water. The cleaning roots are air dried at room temperature for
aboutS-10hrs.
The parts are pulverized to a powder. Extracting the fine powder
obtained from pulverizing, with hot water at a temperature not exceeding
40 C and collecting the filtrate and dried to; as the extract of Abroma
augustus.
The total extract (5:1) should contain 2.4% of tannin and 0. 15o/o alkaloids.
Magnesium is also present.
The process extraction involves the following steps of: obtaining fresh
mature bark of the tree of saracca indica and cleaning the obtained bark
with distilled water. The cleanined bark is dried at temperature not more
than 40 C for about 8 - 10 hrs. The bark is collected and pulverized into
a fine powder.
Extracting the fine powder obtained from pulverizing, with hot water
(temperature not more than 50 C) and collecting the filtrate which is air
dried; preparing acetic acid solution from the extract The other fraction
(5:1) Sarraca indica, aqueous extracts of bark contains total 30% tannin
with leucocyanide as biochemical marker compound.
Fresh mature stem and roots of Asparagus officinalis, are selected and
removed for extraneous matters. The parts are washed and air dried for
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a time period of 8-10 hrs. The dried parts are pulverized in a powderform
and then extracted with water at temperature not more than 40 C lor 8-12
hrs. The extract fraction are collected and evaporated for extra water
content. The extract is air dried. Asparagus officinalis extracts contain
alkaloids 0.15o/o as asparagine and shatavarine as biochemical marker
compound with 15o/o total saponin.
Fresh bark of mature trees Symplocos racemosa, are selected and air
dried. The dried bark is pulverized into powder form and sieved. The
dried parts are pulverized in a powder form and then extracted with water
at temperature not more than 50 C for 8-12 hrs. The extract fraction are
collected and evaporated for extra water content. The extract is air dried.
Symplocos racemosa extract fraction contains total 0.5o/o alkaloids with (-
)-epiafzelechin and symposide ((-)- epiafzelechin-7-B- dglucopyranoside)
that are flavan and flavan glycosides.
The total aqueous extract fractions to be formulated are of Abroma
augustus, Sarraca indica, Symplocos racemosa and Asparagus officinalis
are mixed in the below given ratio. The extract fraction of
Abromaaugustus contains total 2.4o/o of tannin and 0.15% alkaloids,
Abromine (CoHrzNOz), friedelin, Abromasterol as biochemical marker
compound. Sarracaindica extracts fraction of bark contains total 30%
tannin with leucocyanide as biochemical marker compound. Symplocos
racemosa extract fraction contains total 0.5o/o alkaloids with f-)-
epiafzelechin and symposide ((-)-epiafzelechin-7-B -d-glucopyranoside)
that are flavan and flavan glycosides, respectively as biochemical marker
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compound. Asparagus officinalis contains alkaloids 0.15% as asparagine
and shatavarine as biochemical marker compound with 15% total
saponin.
Al! the extracts are mixed in dry form to constitute a powder dosage form
with suitable diluent, containing following fractions:
Abroma augustus-1 00-200 mg, 2040o/o
Sarraca indica- 300-400 mg, 25-40o/o
Symplocos racemosa-SO-1 00 mg, 15-25 o/o
Asparagus officinalis-250-350 mg, 10-20 o/o
Suitable diluents for the oral dosage form: Quantity sufficient to make 1.0
gm This formulation is well proven for indications of adolescent girl
menstrual cycle regulation, optimal functioning of endometrium, and ovary
and neuro hormonal regulation.
The processes described above can be scaled up to produce larger
quantities of extracts. The details provided for preparation of the following
abstracts reflect the presently preferred method for extract preparations
and not be consider as limiting. The quantites and times described below
can be varied substantially to provide suitable extracts.

WE CLAIM:
1. A herbal nutraceutical formulation for adolescent girls comprising:
20 - 40o/o Abroma augustus,
5540o/o Sarraca indica,
15-25% Symplocos racemosa and
1 0-20o/o As pa ra g us Offici n a I is
2. A method for preparing herbal neutraceutical formulation comprising:
obtaining and cleaning fresh matter roots of Abroma augustus,
drying and pulverizing the clean roots,
subjecting the pulverized powder to the step of extraction in hot water,
drying the filtrate,
obtaining and cleaning the mature bark of the tree of Saracea indica,
drying the cleaned bark,
subjecting the dried bark to the step of putverization into form powder
extracting them fine powder with hot water and collecting the filtrate,
preparing acetic acid solution from the extract and drying the same,
obtaining and cleaning the stem and roots of Asparagus officinatis
drying and then pulverizing the clean stem and roots,
subjecting the powder to the step of extraction with water drying the extract
collecting and cleaning the mature bark of symplocos racemosa,
drying and then pulverizing the cleaned bark,
subjecting the powder form of the bark to the step of extraction with water,
collecting the extract fraction and drying the same,
mixing all the extract in the following fractions:
100-200 mg of Abroma augustus
300400 mg of Sarraca indica
50-100 mg of Symplocos racemosa
250-350 mg of Asparagus officinalis
3. The method as claimed in claim 2, wherein the step of extraction of Abroma
augustus is done at a temperature not exceeding 40oC.
4. The method as claimed in claim 2, wherein the bark of Saracea indica is
dried at temperature not more than 40oC for about 8 to 10 hours.
5. The method as claimed in claim 2, wherein the step of extraction of
Saracea indica is done at a temperature of not more than 50oC.
6. The method as claimed in claim 2, wherein the stem and roots of
Asparagus Officinalis is dried for a time period of 8-10 hours.
7. The method as claimed in claim 2, wherein the step of extraction of
Asparagus Officinalis is prepared for 8 - 12 hours at a temperature not
more than 40oC.
8. The method as claimed in claim 2, wherein the step of extraction of
Symplocos racemosa is prepared for 8 - 12 hours at a temperature not
more than 50oC.