Abstract: The invention describes a method of extraction from Withania somnifera (WS) and one or more fractions containing the pharmacologically active ingredients extracted from WS using the method of extraction. A fraction predominantly includes Withaferin-A along with other pharmacologically active ingredients of WS. Another fraction includes additional pharmacologically active ingredients of WS. The method includes soaking a plant material of WS in a mixture of aqueous alcohol and chloroform for a pre¬determined time period and then filtering the mixture to obtain a multi-layered filtrate and a first residue. Thereafter, the multi-layered filtrate is separated into an aqueous alcohol layer and a chloroform layer, Subsequently, the method includes concentrating the aqueous alcohol layer and chloroform layer by evaporation to obtain a hydro-alcoholic fraction and a chloroform fraction. The method further includes dissoluting the chloroform fraction in hexane to get a hexane soluble fraction and a second residue. The second residue obtained by the above method constitutes the fraction including predominantly Withaferin A along with other pharmacologically active ingredients of WS. The hydro-alcoholic fraction obtained by the above method constitutes another fraction including additional pharmacologically active ingredients of WS.
| # | Name | Date |
|---|---|---|
| 1 | 1284-MUM-2009-CORRESPONDENCE(IPO)-(FER)-(26-11-2012).pdf | 2012-11-26 |
| 2 | 1284-MUM-2009-SPECIFICATION(AMENDED)-(25-11-2013).pdf | 2013-11-25 |
| 3 | 1284-MUM-2009-REPLY TO EXAMINATION REPORT(25-11-2013).pdf | 2013-11-25 |
| 4 | 1284-MUM-2009-PETITION UNDER RULE-137(25-11-2013).pdf | 2013-11-25 |
| 5 | 1284-MUM-2009-OTHER DOCUMENT(25-11-2013).pdf | 2013-11-25 |
| 6 | 1284-MUM-2009-FORM PCT-ISA-237(25-11-2013).pdf | 2013-11-25 |
| 7 | 1284-MUM-2009-FORM PCT-IB-373(25-11-2013).pdf | 2013-11-25 |
| 8 | 1284-MUM-2009-FORM 3(25-11-2013).pdf | 2013-11-25 |
| 9 | 1284-MUM-2009-FORM 1(25-11-2013).pdf | 2013-11-25 |
| 10 | 1284-MUM-2009-CLAIMS(AMENDED)-(25-11-2013).pdf | 2013-11-25 |
| 11 | 1284-MUM-2009-ABSTRACT(25-11-2013).pdf | 2013-11-25 |
| 12 | 1284-MUM-2009-CORRESPONDENCE-(30-03-2016).pdf | 2016-03-30 |
| 13 | 1284-MUM-2009_EXAMREPORT.pdf | 2018-08-10 |
| 14 | 1284-mum-2009-form 5.pdf | 2018-08-10 |
| 15 | 1284-MUM-2009-FORM 5(24-7-2009).pdf | 2018-08-10 |
| 16 | 1284-MUM-2009-FORM 5(22-5-2009).pdf | 2018-08-10 |
| 17 | 1284-mum-2009-form 3.pdf | 2018-08-10 |
| 18 | 1284-mum-2009-form 26.pdf | 2018-08-10 |
| 19 | 1284-mum-2009-form 2.pdf | 2018-08-10 |
| 21 | 1284-mum-2009-form 2(title page).pdf | 2018-08-10 |
| 22 | 1284-MUM-2009-FORM 2(TITLE PAGE)-(PROVISIONAL)-(22-5-2009).pdf | 2018-08-10 |
| 23 | 1284-MUM-2009-FORM 2(TITLE PAGE)-(21-5-2010).pdf | 2018-08-10 |
| 24 | 1284-mum-2009-form 2(21-5-2010).pdf | 2018-08-10 |
| 25 | 1284-MUM-2009-FORM 18(21-5-2010).pdf | 2018-08-10 |
| 26 | 1284-mum-2009-form 1.pdf | 2018-08-10 |
| 27 | 1284-MUM-2009-FORM 1(24-7-2009).pdf | 2018-08-10 |
| 28 | 1284-MUM-2009-DRAWING(21-5-2010).pdf | 2018-08-10 |
| 29 | 1284-mum-2009-description(provisional).pdf | 2018-08-10 |
| 31 | 1284-MUM-2009-DESCRIPTION(COMPLETE)-(21-5-2010).pdf | 2018-08-10 |
| 32 | 1284-mum-2009-correspondence.pdf | 2018-08-10 |
| 33 | 1284-MUM-2009-CORRESPONDENCE(IPO)-(HEARING NOTICE)-(1-3-2016).pdf | 2018-08-10 |
| 34 | 1284-MUM-2009-CORRESPONDENCE(IPO)-(DECISION)-(28-3-2016).pdf | 2018-08-10 |
| 35 | 1284-MUM-2009-CORRESPONDENCE(IPO)-(7-10-2009).pdf | 2018-08-10 |
| 36 | 1284-MUM-2009-CORRESPONDENCE(24-7-2009).pdf | 2018-08-10 |
| 37 | 1284-MUM-2009-CORRESPONDENCE(21-5-2010).pdf | 2018-08-10 |
| 38 | 1284-MUM-2009-CLAIMS(21-5-2010).pdf | 2018-08-10 |
| 39 | 1284-mum-2009-abstract.pdf | 2018-08-10 |
| 41 | 1284-MUM-2009-ABSTRACT(22-5-2009).pdf | 2018-08-10 |
| 42 | 1284-MUM-2009-ABSTRACT(21-5-2010).pdf | 2018-08-10 |