Claims:CLAIM
1. A method for a periodontal wound repair, the method comprises:
extraction of stem cells, the extraction of stem cells comprises
obtaining blood from a at least one patient in a at least one first tube, wherein the at least one first tube contain cell separator gel,
the at least one first tube with blood is centrifuged and the blood is separated into an at least one upper layer and a at least one lower layer, where in the at least one upper layer and the at least one lower layer are separated from each other through cell separator gel,
the at least one upper layer in at least one first tube includes supernatant plasma and stem cell suspension, wherein the supernatant plasma lay above in the at least one upper layer and the stem cell suspension present near to the cell separator gel in the at least one upper layer,
the at least one lower layer in at least one first tube includes red blood cells, and
the stem cells obtained from the stem cell suspension of the upper layer in a range of 0.5-1ml;
preparation of glue, the preparation of glue comprises
stem cells obtained in the extraction of stem cells poured into the at least one second tube,
blood obtained from the at least one patient poured into the at least one second tube, wherein the at least one second tube contain cell separator gel,
the at least one second tube is centrifuged, the blood and stem cell mixture is separated into a at least one supernatant layer and a at least one pellet layer,
the at least one supernatant layer and the at least one pellet layer are separated from each other through cell gel separator,
the at least one supernatant layer comprises of supernatant plasma and platelet suspension,
the at least one pellet layer in at least one second tube includes red blood cells, and
the glue is formed after centrifugation in the at least one supernatant layer, wherein the glue is formed due to fibrin polymerization with stem cells;
wherein the glue is taken and used in the periodontal tissue repair.
2. The method claimed in claim 1, wherein the 8ml blood is taken from the at least one patient for the extraction of stem cells and preparation of glue.
3. The method claimed in claim 1, wherein the at least one first tube is centrifuged at 3400 rpm for 6 minutes.
4. The method claimed in claim 1, wherein the at least one second tube is centrifuged for 5 minutes at 3400 rpm.
5. The method claimed in the claim 1, where in the same patient blood is used for the extraction of stem cells and preparation of glue.
6. The method claimed in claim 1, wherein the at least one first tube and the at least one second tube are vacuum collection tubes.
, Description:A Method for Periodontal Wound Healing
FIELD OF THE INVENTION
The present invention relates to a method for a wound repair in patient and more particularly relates to method for a periodontal wound repair in patient with stem cells.
BACKGROUND OF THE INVENTION
In the present age the stem cells brings a revolutionary change in the medical field. The stem cells are known for their totipotency. The stem cells are of different type and obtained from the different parts of the body. One of the types of the stem cells is mesenchymal stem cells. The mesenchymal stem cells are multi potent stem cells which are present in vivo in organism body. The mesenchymal stem cells are able to differentiate in various types of body cells and also help in tissue repair mechanism. In case of the wound healing the stem cells play a major role. The major factor in the wound healing is the growth factors. The stem cells are rich in the growth factors which help in the wound recovery. Now a day’s various procedures are present for wound healing such as tissue engineering, cell based techniques, biomimetic nanoscaffold fabrication and ERT. But the success rate of the present procedure is highly variable and these procedures are highly complex. The stem cells can be used in repair of various body tissues, since the stem cells are rich in growth factors.
US20070231297A1 discloses a method for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived there from (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived there from, cell products derived there from, each alone or in combination.
US7309232B2 discloses methods, apparatus and kits for regenerating dental tissue in vivo that are useful for treating a variety of dental conditions, exemplified by treatment of caries.
The invention uses tissue scaffold wafers, preferably made of PGA, PLLA, PDLLA or PLGA dimensioned to fit into a hole of corresponding sized drilled into the tooth of subject to expose dental pulp in vivo. In certain embodiments the tissue scaffold wafer further comprises calcium phosphate and fluoride. The tissue scaffold wafer may be secured into the hole with a hydrogel, a cement or other suitable material. Either the wafer or the hydrogel or both contain a morphogenic agent, such as a member encoded by the TGF-ß supergene family, that promotes regeneration and differentiation of healthy dental tissue in vivo, which in turn leads to remineralization of dentin and enamel. The tissue scaffold may further include an antibiotic or anti-inflammatory agent.
US6767740B2 discloses Methods and apparatus for recovering dental pulp from dentition of a donor are disclosed, wherein the pulp from within extracted teeth utilizing such methods and apparatus is harvested, while preserving a sterile environment and avoiding trauma and infection, and stem cells, dendritic cells, and other cells isolating from the pulp, and the various cells propagated and expanded for subsequent use in repair or regeneration of tissues of the body, for therapeutic treatments, and other medical purposes.
In the existing inventions the repair process of organs is highly complex and varies a lot. The success rate of the current inventions is low. The costing procedure of the existing invention is also very high. The need therefore has arisen to develop a novel organ/tissue repair process which is simple in nature and produces high result. The present invention overcomes all drawbacks of the existing inventions hence there is need of the present invention.
OBJECTIVE OF THE INVENTION
The main objective of the present invention is to regenerate and repair the tissues particularly in periodontal tissues.
The principal objective of the present invention is to use stem cells in the periodontal wound healing.
Another objective of the present invention is to use the cell growth factors in the wounded area for tissue regeneration or repair mechanism.
Yet another objective of the present invention is to use patient blood for isolation of stem cells for wound healing.
Yet another objective of the present invention is to reduce the cost of operation in tissue wound healing.
Yet another objective of the present invention is to achieve higher regeneration rate of the wound healing process.
Further objectives, advantages and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example and appropriate reference to accompanying drawings.
SUMMARY OF THE PRESENT INVENTION
The present invention relates to a method for a periodontal repair, the method comprises of two steps:
Extraction of stem cells, the extraction of stem cells comprises
Blood is taken from a patient in a first tube. The first tube contains cell separator gel. The first tube is centrifuged and the blood is separated into a upper layer and a lower layer. The upper layer and the lower layer are separated from each other through cell separator gel. The upper layer in the first tube consists of supernatant plasma and stem cell suspension. The supernatant plasma present above in the upper layer and the stem cell suspension present near to the cell separator gel. The lower layer in the first tube includes red blood cells. The stem cells extracted from the upper layer.
Preparation of glue, the preparation of glue comprises
The stem cells obtained in the extraction of stem cell procedure poured into a second tube. The blood obtained from the patient pour into the second tube. The second tube contains cell separator gel. The second tube centrifuged. The blood and stem cell mixture is separated into a supernatant layer and a pellet layer. The supernatant layer and the pellet layer are separated from each other through cell gel separator. The supernatant layer comprises of supernatant plasma and platelet suspension. The glue is formed in the supernatant layer after centrifugation.
One advantage of the present invention is that the present invention provides an effective method for the periodontal wound repair.
Yet another advantage of the present invention is that the present invention repairs the soft and osseous tissue.
Yet another advantage of the present invention is that the present invention uses biological active protein like fibrin, fibronectin and vitronectin for tissue repair.
Yet another advantage of the present invention is that the present invention helps in the regeneration periodontal intrabony defect.
Yet another advantage of the present invention is to ease the root coverage procedure.
Yet another advantage of the present invention is that the present invention provides proper socket preservation repair for the dental defects.
Yet another advantage of the present invention is that the present invention helps in the dental implant placement procedure.
Yet another advantage of the present invention is that the present invention is very helpful in the periapical surgical procedure.
Yet another advantage of the present invention is that the present invention is very effective in the immediate implant placement procedure.
Yet another advantage of the present invention is that the operational and running cost of the present invention is very low as compare to the other procedures.
Yet another advantage of the present invention is that the present invention achieves higher success rate with low operation cost.
Yet another advantage of the present invention is that the present invention can be arrange and implemented in very short time.
Further objectives, advantages and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example and appropriate reference to accompanying drawings.
DETAILED DESCRIPTION OF THE INVENTION
While this invention is susceptible to embodiment in many different forms, there is shown in the drawings and will herein be described in detail specific embodiments, with the understanding that the present disclosure of such embodiments is to be considered as an example of the principles and not intended to limit the invention to the specific embodiments shown and described. In the description below, like reference numerals are used to describe the same, similar or corresponding parts in the several views of the drawings. This detailed description defines the meaning of the terms used herein and
specifically describes embodiments in order for those skilled in the art to practice the invention.
Definition
The terms “a” or “an”, as used herein, are defined as one or as more than one. The term “plurality”, as used herein, is defined as two or as more than two. The term “another”, as used herein, is defined as at least a second or more. The terms “including” and/or “having”, as used herein, are defined as comprising (i.e., open language). The term “coupled”, as used herein, is defined as connected, although not necessarily directly, and not necessarily mechanically.
The term “comprising” is not intended to limit inventions to only claiming the present invention with such comprising language. Any invention using the term comprising could be separated into one or more claims using “consisting” or “consisting of” claim language and is so intended. The term “comprising” is used interchangeably used by the terms “having” or “containing”.
Reference throughout this document to “one embodiment”, “certain embodiments”, “an embodiment”, “another embodiment”, and “yet another embodiment” or similar terms means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of such phrases or in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics are combined in any suitable manner in one or more embodiments without limitation.
The term “or” as used herein is to be interpreted as an inclusive or meaning any one or any combination. Therefore, “A, B or C” means any of the following: “A; B; C; A and B; A and C; B and C; A, B and C”. An exception to this definition will occur only when a
combination of elements, functions, steps or acts are in some way inherently mutually exclusive.
As used herein, the term “stem cells” generally refers to, but not limited to, undifferentiated biological cells that can differentiate into specialized cells and can divide (through mitosis) to produce more stem cells. They are found in multicellular organisms. Adult stem cells are frequently used in various medical therapies (e.g., bone marrow transplantation). Stem cells can now be artificially grown and transformed (differentiated) into specialized cell types with characteristics consistent with cells of various tissues such as muscles or nerves.
As used herein, the term “blood” generally refers to, but not limited to, a body fluid in humans and other animals that delivers necessary substances such as nutrients and oxygen to the cells and transports metabolic waste products away from those same cells.
As used herein, the term “tube” generally refers to, but not limited to, a sterile glass tube with a colored rubber stopper creating a vacuum seal inside of the tube facilitating the drawing of a predetermined volume of liquid. The tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing. The tubes are used for but not limited to collection of blood.
As used herein, the term “cell separator gel” generally refers to, but not limited to, serum separating as physiological inert gel material, used for medical examination, application in biological examination, immunological examination and more gel with increase of serum liquid surface, preventing blood and serum material between interactions, protesting serum biochemical nature and chemical composition without obvious changing for long time.
As used herein, the term “plasma” generally refers to, but not limited to, a yellowish coloured liquid component of blood that normally holds the blood cells in whole blood in
suspension; this makes plasma the extracellular matrix of blood cells. It makes up about 55% of the body's total blood volume.
As used herein, the term “supernatant” generally refers to, but not limited to, the liquid lying above a solid residue after crystallization, precipitation, centrifugation, or other process.
As used herein, the term “red blood cell” generally refers to, but not limited to, the most common type of blood cell and the vertebrate's principal means of delivering oxygen (O2) to the body tissues—via blood flow through the circulatory system. RBCs take up oxygen in the lungs, or gills of fish, and release it into tissues while squeezing through the body's capillaries.
As used herein, the term “polymerization” generally refers to, but not limited to, a process of reacting monomer molecules together in a chemical reaction to form polymer chains or three-dimensional networks.
As used herein, the term “centrifuge” generally refers to, but not limited to, a piece of equipment that puts an object in rotation around a fixed axis (spins it in a circle), applying a potentially strong force perpendicular to the axis of spin (outward). The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances and particles to move outward in the radial direction.
As used herein, the term “polymerization” generally refers to, but not limited to, a process of reacting monomer molecules together in a chemical reaction to form polymer chains or three-dimensional networks.
In preferred embodiment, the present invention relates to method for a periodontal wound repair, the method includes:
extraction of stem cells, the extraction of stem cells comprises
blood is obtained from a patient in a first tube,
the first tube with blood is centrifuged and the blood is separated into a upper layer and a lower layer,
the upper layer and the lower layer are separated from each other through cell separator gel,
the supernatant plasma present in upper portion of the upper layer and the stem cell suspension present in the lower portion of the upper layer near to the cell separator gel, and
the stem cells obtained from the stem cell suspension of the upper layer in a range of 0.5-1ml;
preparation of glue, the preparation of glue comprises
the stem cells obtained pour into a second tube,
the blood obtained from the same patient and poured into the second tube,
the second tube is centrifuged, the blood and stem cell mixture is separated into a supernatant layer and a pellet layer,
the supernatant layer and the pellet layer are separated from each other through cell gel separator.
the supernatant layer comprises of supernatant plasma and platelet suspension,
the pellet layer in second tube includes red blood cells,
the glue is formed after centrifugation in the supernatant layer, and
the glue is extracted from the supernatant layer and used in the tissue repair.
In an embodiment of the present invention, the present invention relates to method for a periodontal wound repair, the method includes:
extraction of stem cells, the extraction of stem cells comprises
blood is obtained from a patient in a first tube, the first tube contain cell separator gel,
the first tube with blood is centrifuged and the blood is separated into a upper layer and a lower layer, the upper layer and the lower layer are separated from each other through cell separator gel,
the upper layer in the first tube consist of supernatant plasma and stem cell suspension, The supernatant plasma present in upper portion of the upper layer and the stem cell suspension present in the lower portion of the upper layer near to the cell separator gel,
the red blood cells are present in the lower layer in the first tube, and
the stem cells obtained from the stem cell suspension of the upper layer in a range of 0.5-1ml;
preparation of glue, the preparation of glue comprises
the stem cells obtained pour into a second tube,
the blood obtained from the same patient and poured into the second tube, the second tube contains cell separator gel,
the second tube is centrifuged, the blood and stem cell mixture is separated in a supernatant layer and a pellet layer due to centrifugation,
the supernatant layer and the pellet layer are separated from each other through cell gel separator,
the supernatant layer comprises of supernatant plasma and platelet suspension,
the pellet layer in second tube includes red blood cells,
the glue is formed due to fibrin polymerization with stem cells after centrifugation in the supernatant layer, and
the glue is extracted from the supernatant layer and used in the tissue repair.
Further objectives, advantages and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed present invention are illustrated by way of example and appropriate reference to accompanying drawings. Those skilled in the art to which the present invention pertains may make modifications resulting in other embodiments employing principles of the present invention without departing from its spirit or characteristics, particularly upon considering the foregoing teachings. Accordingly, the described embodiments are to be considered in all respects only as illustrative, and not restrictive, and the scope of the present invention is, therefore, indicated by the appended claims rather than by the foregoing description or drawings. Consequently, while the present invention has been described with reference to particular embodiments, modifications of structure, sequence, materials and the like apparent to those skilled in the art still fall within the scope of the invention as claimed by the applicant.