FIELD OF THE INVENTION
The present invention generally relates to hygiene technologies and more particularly, to a method of formation of natural remineralizing toothpaste and a composition thereof.
BACKGROUND OF THE INVENTION
Toothpaste is a dentifrice used in the form of a gel or paste to maintain the cleanliness and aesthetics of the teeth and the overall health of the oral cavity. It is usually an abrasive in the form of paste, gel or powder which removes food particles and dental plaque from the tooth surface, cures bad breath problems and prevents gum diseases and tooth decay.
Tooth decay or caries is a chronic disease which usually affects hard tissues of a tooth. The decay or demineralization process is due to an interaction between fermentable sugars from food and acid-producing bacteria. The variety of carbohydrates consumed gets deposited on the tooth surfaces in the form of biofilm or plaque. This layer of plaque is a good source of nutrition for the cariogenic bacteria to act on and produce acids, thus lowering the pH. Prolonged periods of low pH gradually lead to mineral loss from the hard tissues of teeth. Some other factors like inadequate flow of saliva, poor oral hygiene, inappropriate feeding methods of infants and insufficient exposure to fluoride synergistically participate in the sugar – bacteria interaction for further mineral loss. Dental caries affects individuals at any point of their life. It can also severely attack primary teeth in early childhood and thus, a right approach is needed for its prevention.
Over the years, to develop a correct approach for caries prevention, a link has been established between fluoride and oral health. Today amongst the several methods, fluoride application has emerged to be one of the key approaches in caries prevention. Fluoride promotes remineralization of tooth surfaces and hence, nowadays, it is delivered to the oral cavity in various forms such as toothpastes, mouth rinses, varnish, gels and fluoridated water and milk.
Commercially available fluoridated toothpastes have to be used with a lot of caution especially in children. Accidental swallowing of the toothpaste can lead to many dental problems like fluorosis and enamel defects. Chronic ingestion can even cause systemic disorders. Parental supervision is strictly required when using such products in terms of both the amount used and the appropriate method. Even after proper guidance children are still susceptible to swallow the product. So, use of unflavoured fluoridated toothpastes is sometimes recommended but that can even lead to children avoiding brushing. Hence, to overcome such existing disadvantages and demerits of the available fluoridated toothpastes, a formulation of remineralizing toothpaste with all natural elements has been proposed. The composition of the suggested toothpaste is safe and purely herbal. It provides same benefits as fluoride and prevents tooth decay.
Regular use of fluoridated toothpastes is an excellent method for caries control not only in adults but also in children. With the increase in fluoride concentration, its effect of caries prevention also increases. But excessive fluoride use can lead to some adverse reactions. Even inappropriate use of the permissible concentration can also cause harmful effects. Adults using such toothpastes are self-sufficient to keep a check on its appropriate use. But the problem arises in children who are very susceptible to develop caries due to incorrect feeding habits and many other factors. So, regular use of fluoridated toothpaste in children becomes very important to control and prevent the tooth decay process. While doing so, it can give rise to a number of adverse reactions. High concentration of fluoride has been attributed to be a cause of dental fluorosis and enamel defects. Fluorosis is a cosmetic condition affecting the teeth. In the first eight years of life, when the permanent teeth are in their formative stage, overexposure to fluoride can lead to dental fluorosis. It can be a pathologic condition paving the path for teeth having hypo mineralized enamel.
Therefore, there is a need in the art for a method of formation of natural remineralizing toothpaste and a composition thereof.
OBJECT OF THE INVENTION
An object of the present invention is to provide a method of formation of natural remineralizing toothpaste.
Another object of the present invention is to provide a composition of natural remineralizing toothpaste.
Yet another object of the present invention is to develop an anti-cariogenic toothpaste by substituting fluoride
Yet another object to of the present invention is to develop chemical-free, flavoured anti-cariogenic toothpaste for teeth strengthening.
Yet another object of the present invention is to develop a toothpaste that will strengthen tooth enamel and protect it from acid attack.
Yet another object of the present invention is to develop a chemical-free, herbal toothpaste.
Yet another object of the present invention is to develop a chocolate flavoured toothpaste for children for easy acceptance.
SUMMARY OF THE INVENTION
Embodiments of the present invention discloses a method of formation of natural remineralizing toothpaste and a composition thereof.
According to a first aspect of the present invention, a method of formation of natural remineralizing toothpaste is provided. The method comprises steps of mixing a predetermined quantity of bentonite clay and water to form a paste, heating a predetermined quantity of cocoa butter pieces thereby melting the cocoa butter, adding the melted cocoa butter into the paste forming a first mixture, adding a predetermined quantity of cocoa powder, a predetermined quantity of apple powder, a predetermined quantity of celery juice, a predetermined quantity of honey and a predetermined quantity of a foaming agent to the first mixture, forming a second mixture, and mixing the second mixture to get a smooth texture thereby forming a toothpaste.
In accordance with an embodiment of the present invention, for formation of 100 grams of the paste, wherein the predetermined quantity of cocoa powder is in range of 10 grams to 50 grams.
In accordance with an embodiment of the present invention, for formation of 100 grams of the paste, wherein the predetermined quantity of apple powder is in range of 10 grams to 50 grams.
In accordance with an embodiment of the present invention, for formation of 100 grams of the paste, wherein the predetermined quantity of celery juice is in range of 10 ml to 50 ml.
In accordance with an embodiment of the present invention, for formation of 100 grams of the paste, wherein the predetermined quantity of cocoa butter is in range of 10 grams to 50 grams.
In accordance with an embodiment of the present invention, for formation of 100 grams of the paste, wherein the predetermined quantity of honey is in range of 5 grams to 25 grams.
In accordance with an embodiment of the present invention, for formation of 100 grams of the paste, wherein the predetermined quantity of bentonite clay is in range of 3 grams to 9 grams.
In accordance with an embodiment of the present invention, for formation of 100 grams of the paste, wherein the predetermined quantity of foaming agent in range of 0.2 gram to 3 grams.
According to a second first aspect of the present invention, a composition of natural remineralizing toothpaste is provided. The composition comprises a predetermined quantity of cocoa powder, a predetermined quantity of apple powder, a predetermined quantity of celery juice, a predetermined quantity of cocoa butter, a predetermined quantity of honey, a predetermined quantity of bentonite, and a predetermined quantity of foaming agent.
In accordance with an embodiment of the present invention, for a composition of 100 grams of the paste, the predetermined quantity of cocoa powder in range of 10 grams to 50 grams, the predetermined quantity of apple powder in range of 10 grams to 50 grams, the predetermined quantity of celery juice in range of 10 ml to 50 ml, the predetermined quantity of cocoa butter in range of 10 grams to 50 grams, the predetermined quantity of honey in range of 5 grams to 25 grams, the predetermined quantity of bentonite clay in range of 3 grams to 9 grams, and the predetermined quantity of foaming agent in range of 0.2 gram to 03 grams.
BRIEF DESCRIPTION OF THE DRAWINGS
So that the manner in which the above recited features of the present invention can be understood in detail, a more particular to the description of the invention, briefly summarized above, may be had by reference to embodiments, some of which are illustrated in the appended drawings. It is to be noted, however, that the appended drawings illustrate only typical embodiments of this invention and are therefore not to be considered limiting of its scope, the invention may admit to other equally effective embodiments.
These and other features, benefits and advantages of the present invention will become apparent by reference to the following text figure, with like reference numbers referring to like structures across the views, wherein:
Fig. 1 illustrates a method of formation of natural remineralizing toothpaste, in accordance with an embodiment of the present invention;
Fig. 2 illustrates selected tooth, in accordance with an embodiment of the present invention;
Fig. 3 illustrates application of chocolate toothpaste, in accordance with an embodiment of the present invention;
Fig. 4 illustrates SEM photomicrograph of tooth specimen coated with chocolate toothpaste showing demineralized surface with precipitates of calcified components, in accordance with an embodiment of the present invention;
Fig. 5 illustrates SEM photomicrograph of tooth specimen coated with normal fluoridated toothpaste showing demineralized surface with precipitates of calcified components, in accordance with an embodiment of the present invention;
Fig. 6 illustrates SEM photomicrograph of control tooth specimen showing no prominent precipitates of calcified components, in accordance with an embodiment of the present invention;
Fig. 7 illustrates Elemental analysis of surface (EDX) of tooth sample coated with chocolate flavoured anti-cariogenic toothpaste, in accordance with an embodiment of the present invention;
Fig. 8 illustrates spectrum of tooth surface showing calcium and phosphate deposits in sample coated with chocolate toothpaste, in accordance with an embodiment of the present invention;
Fig. 9 illustrates elemental analysis of surface of tooth sample coated with commercially available fluoride toothpaste, in accordance with an embodiment of the present invention; and
Fig 10 illustrates elemental analysis of surface of control tooth sample, in accordance with an embodiment of the present invention.
DETAILED DESCRIPTION OF THE DRAWINGS
While the present invention is described herein by way of example using embodiments, those skilled in the art will recognize that the invention is not limited to the embodiments of drawing or drawings described and are not intended to represent the scale of the various components. Further, some components that may form a part of the invention may not be illustrated in certain figures, for ease of illustration, and such omissions do not limit the embodiments outlined in any way. It should be understood that the drawings and detailed description thereto are not intended to limit the invention to the particular form disclosed, but on the contrary, the invention is to cover all modification/s, equivalent/s and alternative/s falling within the scope of the present invention as defined by the appended claim. The headings used herein are for organizational purposes only and are not meant to be used to limit the scope of the description or the claim. As used throughout this description, the word "may" be used in a permissive sense (i.e. meaning having the potential to), rather than the mandatory sense (i.e. meaning must).
Further, the words "a" or "an" means "at least one” unless otherwise mentioned. Furthermore, the terminology and phraseology used herein is solely used for descriptive purposes and should not be construed as limiting in scope. Language such as "including", "comprising", "having", "containing", or "involving" and variations thereof, is intended to be broad and encompass the subject matter listed thereafter, equivalents, and additional subject matter not recited, and is not intended to exclude other additives, components, integers or steps. Likewise, the term "comprising" is considered synonymous with the terms "including" or "containing" for applicable legal purposes. Any discussion of documents, acts, materials, devices, articles and the likes are included in the specification solely for the purpose of providing a context for the present invention. It is not suggested or represented that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention.
In this disclosure, whenever an element or a group of elements is preceded with the transitional phrase “comprising”, it is understood that we also contemplate the same composition, element or group of elements with transitional phrases “consisting of”, “consisting”, “selected from the group of consisting of”, “including”, or “is” preceding the recitation of the composition, element or group of elements and vice versa.
This invention described herein may be embodied in many different forms and should not be construed as limited to the embodiment set forth herein. Rather, the embodiment is provided so that this disclosure will be thorough and complete and will fully convey the scope of the invention to those skilled in the art. In the following description, numeric values and ranges are provided for various aspects of the implementations described. These values and ranges are to be treated as examples only and are not intended to limit the scope of the claims. In addition, a number of materials are identified as suitable for various facets of the implementations. These materials are to be treated as exemplary and are not intended to limit the scope of the invention. While the present invention is described herein by way of example using embodiments and illustrative drawings, those skilled in the art will recognize that the invention is not limited to the embodiments of drawing or drawings described and are not intended to represent the scale of the various components. Further, some components that may form a part of the invention may not be illustrated in certain figures, for ease of illustration, and such omissions do not limit the embodiments outlined in any way. It should be understood that the drawings and detailed description thereto are not intended to limit the invention to the particular form disclosed, but on the contrary, the invention is to cover all modifications, equivalents, and alternatives falling within the scope of the present invention as defined by the appended claims.
As used throughout this description, the word "may" is used in a permissive sense (i.e., meaning having the potential to), rather than the mandatory sense, (i.e., meaning must). Further, the words "a" or "an" mean "at least one” and the word “plurality” means “one or more” unless otherwise mentioned. Furthermore, the terminology and phraseology used herein is solely used for descriptive purposes and should not be construed as limiting in scope. Language such as "including," "comprising," "having," "containing," or "involving," and variations thereof, is intended to be broad and encompass the subject matter listed thereafter, equivalents, and additional subject matter not recited, and is not intended to exclude other additives, components, integers or steps. Likewise, the term "comprising" is considered synonymous with the terms "including" or "containing" for applicable legal purposes. Any discussion of documents, acts, materials, devices, articles and the like is included in the specification solely for the purpose of providing a context for the present invention. It is not suggested or represented that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention.
The present invention is described hereinafter by various embodiments with reference to the accompanying drawings, wherein reference numerals used in the accompanying drawing correspond to the like elements throughout the description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiment set forth herein. Rather, the embodiment is provided so that this disclosure will be thorough and complete and will fully convey the scope of the invention to those skilled in the art. In the following detailed description, numeric values and ranges are provided for various aspects of the implementations described. These values and ranges are to be treated as examples only and are not intended to limit the scope of the claims. In addition, a number of materials are identified as suitable for various facets of the implementations. These materials are to be treated as exemplary and are not intended to limit the scope of the invention.
Theobromine is an alkaloid which is derived from cacao plants. It is found in high concentration in cocoa powder, dark chocolates, other chocolate products and also in coffee, tea and other foods. Recent studies have found that theobromine found in cocoa powder and chocolates has remineralizing property and can effectively protect the enamel from acid attacks. Thus, it can be an excellent natural alternative to fluoride and protect children from its toxic effects. Crude extracts of antioxidant rich apple have also shown anti-cariogenic properties and inhibit the adherence of cariogenic bacteria to tooth surfaces. Cocoa butter prevents plaque build-up. All these natural compounds, in different ways, protect the teeth against decay and have high potential to show synergistic anti-decay properties when combined together. Moreover, the chocolate derived theobromine adds a chocolate flavour and colour to the toothpaste, which increases the level of acceptance in children.
A toothpaste basically consists of Abrasives, Surfactants, Antibacterial agents, Flavorants, Remineralizing agents. The Abrasive is a key component of toothpaste. While brushing, it mechanically disrupts the plaque formed on teeth. E.g., aluminium hydroxide, silica. Further, the surfactants are foaming agents enabling even distribution and enhancing the cleansing property of toothpaste. E.g., sodium lauryl sulphate. In addition, the Antibacterial agents are commonly found in toothpastes to reduce plaque formation and prevent gingivitis. E.g., Triclosan. Furthermore, the Flavorants are colours and flavour added to the toothpaste for acceptability and likability. Additionally, remineralizing agents are added to enhance remineralizing properties of saliva and fluorides. Examples of remineralizing agents is calcium phosphate. Moreover, the toothpaste may contain some miscellaneous contents such as some agents are added for some further advantages. E.g., xylitol potassium nitrate.
Figure 1 illustrates a method of formation of natural remineralizing toothpaste, in accordance with an embodiment of the present invention. Composition for natural remineralizing toothpaste comprises, but not limited to, a predetermined quantity of cocoa powder, a predetermined quantity of apple powder, a predetermined quantity of celery juice, a predetermined quantity of cocoa butter, a predetermined quantity of honey, a predetermined quantity of bentonite, and a predetermined quantity of foaming agent.
As shown in figure 1, the method of formulation of the toothpaste starts at step 102 where a predetermined quantity of bentonite clay and water is mixed to form a paste. For formation of every 100 grams of the paste, the predetermined quantity of bentonite clay is in range of 3 grams to 9 grams. The bentonite clay added acts as an abrasive. Abrasives help to remove stains from the surface of the teeth. Without any abrasive, toothpastes would become a slippery gel. Plaque is a sticky layer of bacteria that forms on the surface of the teeth. The plaque can build up both above and below the gums. The plaque causes staining of the teeth. Therefore, function of the bentonite clay as abrasive is to clean the teeth stains.
Next, at step 104, a predetermined quantity of cocoa butter pieces is heated thereby melting the cocoa butter. For formation of 100 grams of the paste, the predetermined quantity of cocoa butter is in range of 10 grams to 50 grams. The cocoa butter is melted at a temperature ranging from 29? to 35?. The cocoa butter pieces My be heated at a temperature ranging from, but not limited to, 70? – 90? to form the melted cocoa butter. Further, at step 106, the melted cocoa butter is added into the paste of the bentonite clay and water, forming a first mixture. The cocoa butter added forms a layer over the teeth surfaces and coats the teeth. Coating of the teeth does not allow plaque to stick on the tooth surfaces.
Furthermore, at step 108, a predetermined quantity of cocoa powder is added to the first mixture. for formation of 100 grams of the paste, the predetermined quantity of cocoa powder is in range of 10 grams to 50 grams. In accordance with an embodiment of the present invention, a compound namely theobromine is found in cocoa. The Theobromine, a bitter alkaloid, is extracted from cocoa. It brings calcium and phosphate ions from saliva together and increases the size of hydroxyapatite crystals of enamel, thus increasing its microhardness. Theobromine is an effective remineralizing agent. In presence of calcium and phosphate in saliva, it forms hydroxyapatite crystallites of increased size which strengthens the enamel and increases its microhardness. This also makes the enamel less susceptible to acid attacks.
In addition, a predetermined quantity of apple powder is added to the first mixture. For formation of 100 grams of paste, the predetermined quantity of apple powder is in range of 10 grams to 50 grams. The apple powder used in the composition are rich in antioxidants and nutrients like phosphorus and thus have anti-cariogenic properties. The apple powder inhibits the adherence of cariogenic bacteria on tooth surfaces and inhibit the pH drop. Apple powder has inhibitory activity of adherence of cariogenic bacteria and cocoa butter prevents plaque build- up. Thus, these compounds together protect teeth against cavitation.
Furthermore, as shown in figure 1, at step 108, a predetermined quantity of celery juice is added to the first mixture. For formation of 100 grams of the paste, the predetermined quantity of celery juice is in range of 10 ml to 50 ml. The celery juice in the composition acts as a desensitizing agent. This compound helps in reduction of pain and tooth sensitivity. The compound blocks pain signals traveling from a nerve in tooth to brain. The celery juice may help when one has holes in the enamel of teeth. Since this exposes the sensitive dentin underneath, sensitive toothpaste can rebuild that layer to protect the dentin.
Additionally, at step 108, a predetermined quantity of honey is added to the first mixture. For formation of 100 grams of the paste, the predetermined quantity of honey is in range of 5 grams to 25 grams. The honey is an effective desensitizing and anti-bacterial agent. It also acts as a good preservative. By fighting the bacteria, honey reduces the amount of acid mouth produces. Without that acid, bacteria can no longer produce dextran, which is essentially the “glue” that helps bacteria attach to the tooth surface. Bacteria become plaque, which eventually can harden into what leads to gum disease and tooth decay. Moreover, addition of honey provides a sweet taste to the toothpaste.
In accordance with an embodiment of the present invention, at step 108, a predetermined quantity of a foaming agent is added to the first mixture. For formation of 100 grams of the paste, the predetermined quantity of foaming agent in range of 0.2 gram to 3 grams. The foaming agent may be saponins from Quillaia Saponaria and Yucca schidigera, coconut oil, and like. However, a person skilled in the art may also appreciate usage of Sodium Lauryl Sulphate, sodium Mon fluorophosphate, and like as foaming agent.
After adding a predetermined quantity of cocoa powder, a predetermined quantity of apple powder, a predetermined quantity of celery juice, a predetermined quantity of honey and a predetermined quantity of a foaming agent to the first mixture, a second mixture is formed. At step 110, the second mixture is mixed to form a smooth consistency to get a smooth texture thereby forming a toothpaste.
Different forms of use:
Toothpaste:
The prepared smooth toothpaste can be stored in a paste form in toothpaste dispensing tubes.
Method of application: Squeeze out the paste on a toothbrush to brush teeth whenever required.
Toothpaste tablets:
The prepared smooth mixture having a paste like consistency is loaded into a piping bag. Take a baking tray and place baking sheet on it. Pipe the mixture on the sheet in small droplet-like structures. Bake them in a preheated oven for about one hour. After completion, the droplets should be non-sticky and dry. Cool them and then store in a jar.
Method of use: Take a toothpaste tablet from jar. Place it in the mouth, Chew the tablet until it is broken up, Brush teeth once the tablet breaks.
Tooth Mousse: Make a smooth paste as mentioned earlier with the following ingredients and proportions. Store the mixture in a tube.
Method of use:
1. Home Application:
• Take a small amount of the mousse on fingertip or cotton bud.
• Apply it on all tooth surfaces.
• Keep it for about 2-5 minutes.
• Spit out.
• Avoid eating for the next 30 minutes.
2. Dental Office Application:
• Take a small amount of the mousse on a preformed tray.
• Place the tray properly in the mouth so that the mousse gets applied on all the tooth surfaces.
• Remove tray.
• Keep it for about 2-5 minutes.
• Spit out.
• Avoid eating for the next 30 minutes.
Experiments performed:
As dental caries has various methods of diagnosis, similarly there are multiple experiments to evaluate the surface remineralization of enamel post the usage of fluoridated toothpaste. Enamel surface examination under scanning electron microscope (SEM) is the most widely used approach to assess enamel remineralization. Microhardness test, confocal microscopy and quantitative fluorescence of enamel have been also documented as other used methods. Micro-focus X-ray computed tomography is also a useful approach for assessing mineral density of bones and teeth.
A few of these methods are often employed when the effect of different fluoridated toothpastes on tooth remineralization are compared. Based on these studies a few experiments, may also be performed to evaluate the effectiveness of the prepared natural re-mineralizing toothpaste.
Scanning Electron Microscopic (SEM) Analysis
SEM analysis is one of the best ways to study the surface structures and alterations of teeth. Thus, this analysis gives a potential insight into the effectiveness of such toothpastes on enamel surface mineralization. SEM analysis of specimen surface and elemental surface analysis (EDX) may be performed.
Tooth preparation: Six teeth specimens with sound enamel may be taken. Figure 2 illustrates selected tooth, in accordance with an embodiment of the present invention. The attached soft tissues may be removed and the teeth thoroughly cleaned with running water. To avoid bacterial contamination the specimens may be stored in tap water with thymol crystals dissolved in alcohol (4mL). The tooth surfaces may be cleaned with pumice stone suspensions washed with running water and dried with a towel. A diamond saw may be used under low speed to remove the root portions and keep only the crown for the analysis. It may be done under water to keep control of the heat produced. 4x4x3 mm enamel-dentin blocks may be prepared from the crowns using diamond saw under water. Then with the help of silicone-carbide papers the enamel surfaces may be grounded and made flat. A window of 2x2 mm of the enamel surface may be exposed and rest surfaces may be covered.
Demineralization: The specimens may be demineralized with the help of 37% phosphoric acid. The enamel surfaces may be immersed in the acid for about 7 days. After the demineralization process, they may be again analysed under SEM.
Application of toothpaste: The 6 specimens may be divided into 3 groups of 2 specimens each. In one group, the natural remineralizing chocolate toothpaste may be applied on the enamel surfaces for 5 minutes for 7 days. In the other group, commercially available fluoride toothpaste may be applied for the same duration. Figure 3 illustrates application of chocolate toothpaste (left) in one group and commercially available fluoride toothpaste (right) in other group. The remaining 2 tooth specimens may be used as control and kept in normal saline.
pH cycling: The 6 specimens underwent a pH cycling regime. The enamel specimens may be alternately placed in remineralization solution (1.5 mM CaCl2, 0.9mM KH2PO4, 130mM KCl; pH=7 with 10mM KOH and 1.0mM NaN3) and demineralization solution (17.8mM CaCl2, 8.8 mM KH2PO4, and 100 mM lactic acid, 1.0 mM NaN3; pH=4.3 with 10mM KOH). During a 24-hour period the blocks may be placed in remineralization solution for 1-hour and demineralization solution for 23-hour and may be washed with water between the alterations. The toothpaste treatments may be done just after demineralization and before remineralization. The toothpastes may be applied once daily for 5 minutes for 7 days.
SEM analysis: After 7 days the enamel surfaces of 3 specimens may be analysed under SEM.
Table 1: Elemental analysis of surfaces of the specimens
ELEMENTS CONTENT OF ELEMENTS (Wt %) IN DIFFERENT SPECIMENS
CHOCOLATE TOOTHPASTE COATED TOOTH COMMERCIAL FLUORIDE TOOTHPASTE COATED TOOTH CONTROL TOOTH SPECIMEN
Calcium (Ca) 15.7 39.8 4.7
Phosphorus (P) 8.6 15.6 2.6
Oxygen (O) 53.7 35.6 28.2
Carbon (C) 21 6.9 47.2
Sodium (Na) 0.6 0.6 4

Results: Theobromine containing chocolate toothpaste demonstrated re mineralizing ability on the enamel surfaces. Thus, it can be quite well speculated that the proposed toothpaste composition could easily replace the use of commercially available fluoridated toothpastes and products. The pure natural composition will be equally effective in remineralization process and will be much safer than the existing compositions. Figure 4 illustrates SEM photomicrograph of tooth specimen coated with chocolate toothpaste showing demineralized surface with precipitates of calcified components, in accordance with an embodiment of the present invention. Further, Figure 5 illustrates SEM photomicrograph of tooth specimen coated with normal fluoridated toothpaste showing demineralized surface with precipitates of calcified components. Furthermore, figure 6 illustrates SEM photomicrograph of control tooth specimen showing no prominent precipitates of calcified components. In addition, Figure 7 illustrates Elemental analysis of surface (EDX) of tooth sample coated with chocolate flavoured anti-cariogenic toothpaste, in accordance with an embodiment of the present invention; Additionally, Figure 8 illustrates spectrum of tooth surface showing calcium and phosphate deposits in sample coated with chocolate toothpaste. Moreover, Figure 9 illustrates elemental analysis of surface of tooth sample coated with commercially available fluoride toothpaste. In addition, Figure 10 illustrates elemental analysis of surface of control tooth sample, in accordance with an embodiment of the present invention.
The present invention provides a number of advantages. The toothpaste is an effective anti-caries agent. It strengthens the enamel and protects it in acidic condition. The toothpaste provides a good chocolaty taste and makes it palatable and acceptable to children. The toothpaste is safe and purely herbal. Ingestion of toothpaste during brushing in children will have no toxic effects. The toothpaste poses no risk of dental fluorosis, enamel defects or systemic toxicity. It provides a coating on teeth and prevents plaque build-up. It provides a soothing effect on teeth and comforts individuals from hypersensitivity. The toothpaste can be used by children and adults safely. There is no strict supervision or guidance required in children. There is no training of “spitting out the toothpaste” is needed in children. Supervision on amount of toothpaste used is also not needed.
Chronic use of fluoridated dental products especially toothpastes will lead to harmful effects in adults as well as in children. Children specially require a supervision and training in using such fluoridated products. Risk of ingestion is very high in them. Thus, it is very needed to use non-fluoridated toothpastes which will provide the anti-cariogenic benefits of fluoride and at the same time will be safe. Thus, the above mentioned toothpaste composition is required in the market today. It has the anti-caries properties of fluoride and a chocolate taste and colour which will be make it attractive to children. Theobromine, a chocolate derivative, which provides all these benefits, should be the active ingredient of choice in future toothpastes.
Various modifications to these embodiments are apparent to those skilled in the art from the description and the accompanying drawings. The principles associated with the various embodiments described herein may be applied to other embodiments. Therefore, the description is not intended to be limited to the embodiments shown along with the accompanying drawings but is to be providing broadest scope of consistent with the principles and the novel and inventive features disclosed or suggested herein. Accordingly, the invention is anticipated to hold on to all other such alternatives, modifications, and variations that fall within the scope of the present invention and the appended claims.

We claim:

1. A method of formation of natural remineralizing toothpaste, the method comprising steps of:
mixing a predetermined quantity of bentonite clay and water to form a paste;
heating a predetermined quantity of cocoa butter pieces thereby melting the cocoa butter;
adding the melted cocoa butter into the paste forming a first mixture.
adding a predetermined quantity of cocoa powder, a predetermined quantity of apple powder, a predetermined quantity of celery juice, a predetermined quantity of honey and a predetermined quantity of a foaming agent to the first mixture, forming a second mixture; and
mixing the second mixture to get a smooth texture thereby forming a toothpaste.
2. The method as claimed in claim 1, for formation of 100 grams of the paste, wherein the predetermined quantity of cocoa powder is in range of 10 grams to 50 grams.
3. The method as claimed in claim 1, for formation of 100 grams of the paste, wherein the predetermined quantity of apple powder is in range of 10 grams to 50 grams.
4. The method as claimed in claim 1, for formation of 100 grams of the paste, wherein the predetermined quantity of celery juice is in range of 10 ml to 50 ml.
5. The method as claimed in claim 1, for formation of 100 grams of the paste, wherein the predetermined quantity of cocoa butter is in range of 10 grams to 50 grams.
6. The method as claimed in claim 1, for formation of 100 grams of the paste, wherein the predetermined quantity of honey is in range of 5 grams to 25 grams.
7. The method as claimed in claim 1, for formation of 100 grams of the paste, wherein the predetermined quantity of bentonite clay is in range of 3 grams to 9 grams.
8. The method as claimed in claim 1, for formation of 100 grams of the paste, wherein the predetermined quantity of foaming agent in range of 0.2 gram to 3 grams.
9. A composition of natural remineralizing toothpaste, the composition comprising:
a predetermined quantity of cocoa powder;
a predetermined quantity of apple powder;
a predetermined quantity of celery juice;
a predetermined quantity of cocoa butter;
a predetermined quantity of honey;
a predetermined quantity of bentonite; and
a predetermined quantity of foaming agent.
10. The composition as claimed in claim 9, wherein for a composition of 100 grams of the paste:
the predetermined quantity of cocoa powder in range of 10 grams to 50 grams;
the predetermined quantity of apple powder in range of 10 grams to 50 grams;
the predetermined quantity of celery juice in range of 10 ml to 50 ml;
the predetermined quantity of cocoa butter in range of 10 grams to 50 grams;
the predetermined quantity of honey in range of 5 grams to 25 grams;
the predetermined quantity of bentonite clay in range of 3 grams to 9 grams; and
the predetermined quantity of foaming agent in range of 0.2 gram to 03 grams.