CLIAMS:1. A method of identification of bioactives that inhibit melanin synthesis, wherein the method comprises:
a. obtaining a bioactive with melanin synthesis inhibition potential;
b. contacting the bioactive from (a) with a cell capable of producing melanin; and
c. assaying the transcript levels of microRNAs
wherein, the up-regulation of microRNAs is indicative of ability of the bioactive to inhibit melanin synthesis.
2. The method as claimed in claim 1, wherein the cell is melanocyte.
3. The method as claimed in claim 1, wherein the microRNAs are selected from the group consisting of miR-BART12, miR-BART13, miR-BART16, miR-BART19-3p, let-7a-5p, let-7b-5p, let-7c, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-100-5p, miR-103a-3p, miR-106b-5p, miR-107, miR-10a-5p, miR-10b-5p, miR-1181, miR-1202, miR-1207-5p, miR-1225-5p, miR-1246, miR-125a-3p, miR-125a-5p, miR-125b-5p, miR-1260a, miR-1260b, miR-1268a, miR-1274a_v16.0, miR-1274b_v16.0, miR-1275, miR-1280, miR-1281, miR-1290, miR-1305, miR-130a-3p, miR-130b-3p, miR-134, miR-135a-3p, miR-138-2-3p, miR-140-3p, miR-146a-5p, miR-148a-3p, miR-148b-3p, miR-150-3p, miR-151a-3p, miR-151a-5p, miR-155-5p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-185-5p, miR-188-5p, miR-1915-3p, miR-193a-3p, miR-193a-5p, miR-193b-3p, miR-193b-5p, miR-1973, miR-197-3p, miR-199a-3p, miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-20b-5p, miR-210, miR-211-5p, miR-21-5p, miR-221-3p, miR-222-3p, miR-22-3p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-30e-5p, miR-3152-3p, miR-31-5p, miR-3162-5p, miR-3195, miR-3196, miR-3198, miR-320a, miR-320b, miR-320c, miR-320d, miR-320e, miR-324-3p, miR-324-5p, miR-331-3p, miR-34a-5p, miR-34b-5p, miR-361-3p, miR-361-5p, miR-362-5p, miR-3651, miR-3652, miR-3653, miR-3656, miR-365a-3p, miR-3663-3p, miR-3665, miR-3679-5p, miR-371a-5p, miR-378_v17.0, miR-3937, miR-423-5p, miR-425-5p, miR-4257, miR-4271, miR-4281, miR-4284, miR-4286, miR-4298, miR-4299, miR-4306, miR-4313, miR-4327, miR-455-3p, miR-483-5p, miR-494, miR-509-3p, miR-513a-5p, miR-513b, miR-513c-5p, miR-514a-3p, miR-514b-5p, miR-532-3p, miR-548c-3p, miR-548f, miR-571, miR-572, miR-574-3p, miR-574-5p, miR-575, miR-584-5p, miR-623, miR-630, miR-638, miR-642b-3p, miR-720, miR-762, miR-874, miR-92a-3p, miR-93-5p, miR-939, miR-940, miR-98, miR-99b-5p, miR-H1*, miR-H18, miR-H6-5p, miR-H8, miR-H10, miR-H24, miR-H6, miR-K12-3, and miR-K12-5*.
4. The method as claimed in claim 3, wherein the microRNA is miR29b-3p or miR23a-3p or miR-27a-3p or miR-27b-3p or miR-155-5p or miR-25-3p or let-7d-5p.
5. The method as claimed in claim 1, wherein the microRNA inhibits the synthesis of proteins involved in melanin synthesis.
6. The method as claimed in claim 5, wherein the proteins are selected from the group consisting of MITF, TYR, TRP1, SLC24A5, SLC45A, and CREB.
7. A composition comprising microRNAs selected from the group consisting of miR-BART12, miR-BART13, miR-BART16, miR-BART19-3p, let-7a-5p, let-7b-5p, let-7c, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-100-5p, miR-103a-3p, miR-106b-5p, miR-107, miR-10a-5p, miR-10b-5p, miR-1181, miR-1202, miR-1207-5p, miR-1225-5p, miR-1246, miR-125a-3p, miR-125a-5p, miR-125b-5p, miR-1260a, miR-1260b, miR-1268a, miR-1274a_v16.0, miR-1274b_v16.0, miR-1275, miR-1280, miR-1281, miR-1290, miR-1305, miR-130a-3p, miR-130b-3p, miR-134, miR-135a-3p, miR-138-2-3p, miR-140-3p, miR-146a-5p, miR-148a-3p, miR-148b-3p, miR-150-3p, miR-151a-3p, miR-151a-5p, miR-155-5p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-185-5p, miR-188-5p, miR-1915-3p, miR-193a-3p, miR-193a-5p, miR-193b-3p, miR-193b-5p, miR-1973, miR-197-3p, miR-199a-3p, miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-20b-5p, miR-210, miR-211-5p, miR-21-5p, miR-221-3p, miR-222-3p, miR-22-3p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-30e-5p, miR-3152-3p, miR-31-5p, miR-3162-5p, miR-3195, miR-3196, miR-3198, miR-320a, miR-320b, miR-320c, miR-320d, miR-320e, miR-324-3p, miR-324-5p, miR-331-3p, miR-34a-5p, miR-34b-5p, miR-361-3p, miR-361-5p, miR-362-5p, miR-3651, miR-3652, miR-3653, miR-3656, miR-365a-3p, miR-3663-3p, miR-3665, miR-3679-5p, miR-371a-5p, miR-378_v17.0, miR-3937, miR-423-5p, miR-425-5p, miR-4257, miR-4271, miR-4281, miR-4284, miR-4286, miR-4298, miR-4299, miR-4306, miR-4313, miR-4327, miR-455-3p, miR-483-5p, miR-494, miR-509-3p, miR-513a-5p, miR-513b, miR-513c-5p, miR-514a-3p, miR-514b-5p, miR-532-3p, miR-548c-3p, miR-548f, miR-571, miR-572, miR-574-3p, miR-574-5p, miR-575, miR-584-5p, miR-623, miR-630, miR-638, miR-642b-3p, miR-720, miR-762, miR-874, miR-92a-3p, miR-93-5p, miR-939, miR-940, miR-98, miR-99b-5p, miR-H1*, miR-H18, miR-H6-5p, miR-H8, miR-H10, miR-H24, miR-H6, miR-K12-3, and miR-K12-5*, wherein said microRNAs inhibit expression of proteins involved in melanin synthesis.
8. The composition as claimed in claim 7, wherein the protein is selected from the group consisting of MITF, TYR, TRP1, SLC24A5, SLC45A, and CREB.
9. A composition comprising a miRNA that inhibits melanin synthesis by inhibiting SLC45A expression, wherein the microRNA is microRNA- 29b-3P. ,TagSPECI:As Attached