This invention relates to a method of Leaf extract of Pedilanthus Tithymaloides for herbal synthesis of TiO2 nanoparticles.
Background of the Invention
US20090061230A1 Synthesis of Titanium Dioxide Nanoparticles discloses the synthesis of nanoparticle having average particle size 25 nm. In this method used hydrolysable halide as a precursor in the synthesis of nanoparticles. The innovation helpful for industrial usefulness of TiO2 nanoparticles.
Research Gap: In this patent chemical methods used in the production TiO2 nano particle but in the present innovation we use the leaf extract of pedilanthus tithymaloides.
WO2012131643A1 Herbal compositions comprising pedilanthus tithymaloides for treatment prevention of gastrointestinal disorder discloses the phytochemical analysis of the extract of the different part of the selected medicinal plant and established that they are very use full in the treatment of gastrointestinal disorder.
Research Gap: In this patent leaf extract of pedilanthus tithymaloides use. In the treatment of the gastrointestinal disorder the but in the present innovation we use the leaf extract of pedilanthus tithymaloides in synthesis of TiO2 nanoparticles.
None of the prior art indicate above either alone or in combination with one another disclose what the present invention has disclosed. Present invention is Leaf extract of Pedilanthus Tithymaloides for herbal synthesis of TiO2 nanoparticles.
SUMMARY OF THE INVENTION
This summary is provided to introduce a selection of concepts, in a simplified format, that are further described in the detailed description of the invention.
This summary is neither intended to identify key or essential inventive concepts of the invention and nor is it intended for determining the scope of the invention.
To further clarify advantages and features of the present invention, a more particular description of the invention will be rendered by reference to specific embodiments thereof, which is illustrated in the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. The invention will be described and explained with additional specificity and detail with the accompanying drawings.
Discloses herein a method of Green synthesis of TiO2 nanoparticles by using this leaf extract comprising the steps of: using Isolated leaf extract as reducing agent in the synthesis of TiO2 nanoparticles; Taking 20 mL titanium tetraisopropoxide in a conical flask and add 5 mL of isolated leaf extract in this solution; placing this flask on the magnetic stirrer keeps the temperature around 60-650C; so that after 6 hours continuous stirring the color of the solution starts to change; wherein this color change indicates the formation of our nanoparticles; after the color of the whole solution changed from green to buff colored stop the stirring and dry the reaction mixture; after 2-3 h now placed the powder in to the muffle furnace at 400oC for about 1 hour; and After one hour of calcinations we obtained a white color Powder of the desired product.
During the last few decades the use of inorganic metal oxide nanoparticles is increasing tremendously. The inorganic metal oxide nanoparticles have very wide range of application like in pharmaceutical industry, food industry in the reproduction of silkworm etc. There are so many physical and chemical methods are available for the synthesis of metal oxide nanoparticles .But the available conventional method creating serious environmental threat as they use lots of carcinogenic chemical in the production of metal oxide nanoparticles. Hence in the last few years interest among the researcher to develop a clean ecofriendly approach for the potentially useful metal oxide nanoparticles by using waste product or by using the extract of the leaf roots and barks
BRIEF DESCRIPTION OF THE DRAWINGS
The illustrated embodiments of the subject matter will be understood by reference to the drawings, wherein like parts are designated by like numerals throughout. The following description is intended only by way of example, and simply illustrates certain selected embodiments of devices, systems, and methods that are consistent with the subject matter as claimed herein, wherein:
Fig. 1: UV analysis
Fig. 2: FT IR Analysis of the fabricated TiO2 NPs
Fig. 3: XRD Analysis
Fig. 4: Morphological analysis of the fabricated nanoparticles
The figures depict embodiments of the present subject matter for the purposes of illustration only. A person skilled in the art will easily recognize from the following description that alternative embodiments of the structures and methods illustrated herein may be employed without departing from the principles of the disclosure described herein.
DETAILED DESCRIPTION OF THE INVENTION
The detailed description of various exemplary embodiments of the disclosure is described herein with reference to the accompanying drawings. It should be noted that the embodiments are described herein in such details as to clearly communicate the disclosure. However, the amount of details provided herein is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the present disclosure as defined by the appended claims.
It is also to be understood that various arrangements may be devised that, although not explicitly described or shown herein, embody the principles of the present disclosure. Moreover, all statements herein reciting principles, aspects, and embodiments of the present disclosure, as well as specific examples, are intended to encompass equivalents thereof.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments. As used herein, the singular forms “a",” “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms “comprises,” “comprising,” “includes” and/or “including,” when used herein, specify the presence of stated features, integers, steps, operations, elements and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and/or groups thereof.
It should also be noted that in some alternative implementations, the functions/acts noted may occur out of the order noted in the figures. For example, two figures shown in succession may, in fact, be executed concurrently or may sometimes be executed in the reverse order, depending upon the functionality/acts involved.
In addition, the descriptions of "first", "second", “third”, and the like in the present invention are used for the purpose of description only, and are not to be construed as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first" and "second" may include at least one of the features, either explicitly or implicitly.
Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which example embodiments belong. It will be further understood that terms, e.g., those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
These and other advantages of the present subject matter would be described in greater detail with reference to the following figures. It should be noted that the description merely illustrates the principles of the present subject matter. It will thus be appreciated that those skilled in the art will be able to devise various arrangements that, although not explicitly described herein, embody the principles of the present subject matter and are included within its scope.
Material and Methods
Isolation of Leaf extract:
Pedilanthus Tithymaloides plant was collected in different part of Dehradun. From the collected plant material the leaves were separated and washed with double distilled water to remove the dust and other particles associated with the leaf.Now the leaf was dry under the shade. After drying the powder of the leaf was prepared by grinding. The powder of the leaf was placed in the soxhlet extractor and isolated the leaf extract by using ethanol as a solvent. After four h of continuous extraction the extracted was isolated and it is collected in the sample tube.
Green synthesis of TiO2 nanoparticles by using this leaf extract:
Isolated leaf extract was used as reducing agent in the synthesis of TiO2 nanoparticles. For the synthesis of TiO2 nanoparticles first Took 20 mL titanium tetraisopropoxide in a conical flask and add 5 mL of isolated leaf extract in this solution. Now placing this flask on the magnetic stirrer keeps the temperature around 60-650C. After 6 hr continuous stirring the color of the solution starts to change. This color change indicates the formation of our nanoparticles. After the color of the whole solution changed from green to buff colored stop the stirring and dry the reaction mixture. After 2-3 h now we placed the powder in to the muffle furnace at 400oC for about 1 h. After one hour of calcinations we obtained a white color Powder of the desired product.
Characterization of the fabricated nanoparticles:
The fabricated nanoparticles was analyzed by using various analytical techniques like UV,FT-IR, XRD and SEM.
UV analysis:
The prepared TiO2 nanoparticles were aanalyzed by several techniques. UV–vis absorbance spectroscopy was used to determine the molecule size and band hole of titanium dioxide nanoparticles that have been incorporated. The absorption spectrum was measure in wavelength range of 200-800 nm and in the ultraviolet absorption spectra of produced TiO2, a prominent absorption band at 400 nm was observed. Our findings are consistent with the published literature, indicating the presence of a titanium dioxide band at 380–400 nm.
FT IR Analysis of the fabricated TiO2 NPs
Fourier transform infra-red spectroscopy (FTIR) technique has been done for the characterization of synthesized TiO2 nanoparticles that explained the formation of these nanoparticles by depicting various functional groups and chemical compounds that were present in them and are responsible for the nanoparticle formation. Figure showing the absorption spectra of synthesized TiO2 nanoparticles. The highest peak is marked at 1107 cm-1 .The band at 1491-1402 cm-1 depicts the bending of C-H functional groups. The band at 1215 cm-1 displays the presence C-O stretching groups of carboxylic acids, esters etc. The stretching vibration of O-H reflecting at the band near 1854-1887 cm-1 indicates the alcohol and phenol groups. Peaks observed at 1630.547 and 1391.910 cm-1 indicate the Ti-O band and Ti-O-Ti stretching mode. The presence of hydroxyl group builds up photocatalytic efficiency of these nanoparticles.
XRD Analysis:
XRD was also used to characterize the prepared nanoparticles. The XRD characterization showed that the prepared nanoparticles have highly crystalline structure with anatase phase . The diffraction angle showed Several peaks were observed at 25.29°, 36.7°, 48.4°, 55.2°, 62.7° and 70.50. The peaks are in sync with JCPDS data (#21-1272) .The diffraction angle at 25.29 closely related to the crystallographic anatase phase of TiO2 . The particle size of the fabricated TiO2 nanoparticles was determine by the Scherrer's equation. The average crystallite size of the herbal synthesized TiO2 nanoparticles was found to be 10 nm.
Morphological analysis of the fabricated nanoparticles:
Scanning electron microscopy (SEM) analysis of these synthesized TiO2 nanoparticles have detected various microstructural properties and features carried by these nanoparticles. Complete information of these nanoparticles from size to roughness of the surface has been detected by SEM analysis. As shown in figure the morphology of the TiO2 nanoparticles was explained from the SEM images that depicted the size of these nanoparticles that resulted in a range between 45-70 nm. The nanoparticles are crystalline in nature and acquire spherical shape and most of them are in the form of clusters with agglomeration which makes it problematic to study and specify each one of the nanoparticles individually. It also depicted the pore distribution and grain distribution, some of the grains came out to be bigger in size.
ADVANTAGES OF THE INVENTION:
A new application of the leaf extract of the selected medicinal plant Pedilanthus Tithymaloides was developed which was very use full to develop a new ecofriendly, cost effective method to synthesized the very useful TiO2 nanoparticles.

WE CLAIM:

1. A method of Green synthesis of TiO2 nanoparticles by using this leaf extract comprising the steps of: using Isolated leaf extract as reducing agent in the synthesis of TiO2 nanoparticles;
Taking 20 mL titanium tetraisopropoxide in a conical flask and add 5 mL of isolated leaf extract in this solution;
placing this flask on the magnetic stirrer keeps the temperature around 60-650C; so that after 6 hours continuous stirring the color of the solution starts to change;
wherein this color change indicates the formation of our nanoparticles; after the color of the whole solution changed from green to buff colored stop the stirring and dry the reaction mixture;
after 2-3 h now placed the powder in to the muffle furnace at 400oC for about 1 hour; and After one hour of calcinations we obtained a white color Powder of the desired product.
2. The method as claimed in claim 1, wherein the absorption spectrum is measured in wavelength range of 200-800 nm and in the ultraviolet absorption spectra of produced TiO2, a prominent absorption band at 380–400 nm is observed.
3. The method as claimed in claim 1, wherein Fourier transform infra-red spectroscopy (FTIR) technique is done for the characterization of synthesized TiO2 nanoparticles; wherein the highest peak is marked at 1107 cm-1 ; wherein the band at 1491-1402 cm-1 depicts the bending of C-H functional groups; wherein the band at 1215 cm-1 displays the presence C-O stretching groups of carboxylic acids, esters; The stretching vibration of O-H reflecting at the band near 1854-1887 cm-1 indicates the alcohol and phenol groups; wherein Peaks observed at 1630.547 and 1391.910 cm-1 indicate the Ti-O band and Ti-O-Ti stretching mode.
4. The method as claimed in claim 1, wherein XRD is also used to characterize the prepared nanoparticles; wherein the diffraction angle showed Several peaks were observed at 25.29°, 36.7°, 48.4°, 55.2°, 62.7° and 70.50; and the peaks are in sync with JCPDS data (#21-1272); wherein the diffraction angle at 25.29 closely related to the crystallographic anatase phase of TiO2; wherein the particle size of the fabricated TiO2 nanoparticles was determine by the Scherrer's equation; and wherein the average crystallite size of the herbal synthesized TiO2 nanoparticles was found to be 10 nm.