Description:FIELD OF INVENTION:
The present invention relates to a method of preparing a poly herbal and poly nutrient formulated supplement for lactating animals to increase milk yield, enhance the quality of milk while significantly improving and safeguarding the health of animal. The name of our product is ECOGOLD BIOKSHEERA.

BACKGROUND OF INVENTION:
The production of milk in animal is at peak when they are in the early stage of lactation i.e., initial 3 to 4 months but it starts declining later on and farmers often complain about the reduction in the ability of animals to produce milk regularly.
The general cause of this can be physiological, nutritional, dairy farm management, metabolic disturbances or due to disease conditions. Normally, improper nutrition and supplementation are the main causes.
Now to overcome the above causes, many ways and medications are currently available in the market and the natural way to increase the milk production is to provide proper nutrition through minerals which are found in plants but the level of nutrition varies field to field and it also depends on the amount of minerals present in the soil. Therefore, it is difficult to give average values for the amount of minerals that might be expected from feeds.
Other than the plant-based nutrition, grains and legumes are also used to increase the milk production but grains are low in calcium but high in phosphorous and legumes are usually good sources of calcium but not phosphorous.
OBJECT OF INVENTION:
The primary objective of the present invention is to provide poly herbal and poly nutrient formulated supplement for lactating animals to increase milk yield naturally without using any kind of chemicals and steroids that can have side effects on the health of the animal.
Another objective of the present invention is to provide poly herbal and poly nutrient formulated supplement for lactating animals wherein, the present invention helps in enhancing the quality of milk while significantly improving and safeguarding the health of animal.
Another objective of the present invention is to provide poly herbal and poly nutrient formulated supplement for lactating animals wherein, the present invention comprises of completely natural galactogogue, which stimulates the activity of alveolar tissue and increase the secretory activity.
Yet another objective of the present invention is to provide poly herbal and poly nutrient formulated supplement for lactating animals wherein, the present invention is providing a cost-effective way to increase the milk yield.
Yet another objective of the present invention is to provide poly herbal and poly nutrient formulated supplement for lactating animals wherein, the present invention helps in improving the digestion, draining out the worms out of stomach naturally, attaining the natural heat cycle of the animal.
Other objects and advantages of the present invention will become apparent from the following description.
BRIEF DESCRIPTION OF FIGURES:
The present invention will be better understood after reading the following detailed description of the presently preferred aspects thereof with reference to the appended drawings, in which the features, other aspects and advantages of certain exemplary embodiments of the invention will be more apparent from the accompanying drawings in which:
Figure 1 illustrates a certificate of analysis comprising of extract compositions.
Figure 2 illustrates a test report of feed mix conducted by Ministry of Agriculture Development and Food Security Botswana National Veterinary Laboratory.
Figure 3 illustrates a test report conducted by SGS India Pvt. Ltd.
Figure 4 illustrates a trial report conducted by Department of Farm Animal Production & Health Faculty of Veterinary Medicine & Animal Science, University of Peradeniya, Srilanka evidencing the increase of milk yield by one litre.
DETAILED DESCRIPTION:
The following description describes various features and functions of the disclosed invention and methods with reference to the accompanying figures. In the figures, similar symbols identify similar components, unless context dictates otherwise. The illustrative aspects described herein are not meant to be limiting. It may be readily understood that certain aspects of the disclosed system, method and apparatus can be arranged and combined in a wide variety of different configurations, all of which are contemplated herein.
These and other features and advantages of the present invention may be incorporated into certain embodiments of the invention and will become fully apparent from the following description and claims or may be learned by the practice of the invention as set forth hereinafter.
Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope of the invention. In addition, descriptions of well-known functions and constructions are omitted for clarity and conciseness.
The terms and words used in the following description and claims are not limited to the bibliographical meanings, but, are merely used to enable a clear and consistent understanding of the invention. Accordingly, it should be apparent to those skilled in the art that the following description of exemplary embodiments of the present invention are provided for illustration purpose only and not for the purpose of limiting the invention.
It is to be understood that the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise.
It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, integers, steps or components but does not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.
The present invention is directed towards to poly herbal and poly nutrient formulated supplement for lactating animals to increase milk yield, enhance the quality of milk while significantly improving and safeguarding the health of animal.
The present invention, a completely natural and herbal feed supplement which does not contain any kind of chemicals and steroids that can have side effects on health of the animal, comprises of laptadenia reticulate, asparagus racemosus, withania somnifera, withania somnifera, ipomea mauritiana, citrus aurantifolia, hemidesmus indicus, galactogogue, lactogenic and anabolic.
In accordance with the present invention, the present invention comprises of completely natural galactogogue, which stimulates the activity of alveolar tissue and increase the secretory activity, providing a cost-effective way to increase the milk yield, improving the digestion, draining out the worms out of stomach naturally, attaining the natural heat cycle of the animal.
Also, the present invention substantially reduces the methane gas production with best digestion of feed and helps in reducing global warming and is eligible for carbon credits, helps in making the skin of animals’ shinny due to the presence of copper, vitamins and minerals and helps in maintaining the milk production of the animal during the summer season.
LEPTADAENIA RETICULATE (JIVANTI or SVARNAJIVANTZ):
The name (Jiv = life) indicates that the plant has the ability to bestow health and vigour. It is considered to be a rasayana and included among the 10 drugs constituting the jivaniya gana or vitalizing group. It is an ingredient of Chyawanprash, a traditional Polyherbal formulation. The major therapeutic importance is its galactogogue action, which has been proved in rats (Anjaria et al, 1975), cows (Anjaria et al, 1974) as well as humans (Patel et ai, 1982). Lactogenic Anabolic & Galactogogue effect (Ravishankar & Shukla 2007).

Lactogenic: Induce, Initiate and Increase secretion of milk by the mammary glands.

Galactogogue: Induces Secretion and continued production of milk by the mammary glands.

Anabolic: It promotes the storage of protein and the growth of tissue, this generative process (anabolism) results in increased lean muscle mass, stronger bones, and a greater energy supply.

Leptadenia Reticulata is a branched twining shrub of family Asclepiadaceae contains many important phytoconstituents of plants. Major constituents of plants are alpha-amyrin, beta-amyrin, ferulic acid, luteolin, diosmetin, rutin, beta-sitosterol, stigmasterol, hentriacontanol, atriterpene alcohol simiarenol, apigenin (Krishna et al., 1975; Subramanian and Lakshmanan, 1977; Sastry et al., 1985). Some other are pregnane glycosides reticulin, deniculatin, leptaculatin. Other are acetyl alcohol, lupanol 3-O diglucoside, leptidine 1, saponins, flavonoid, luteolin, diosmtin and tannin. Leaves contain two resins and also a bitter neutral principle, albuminous and colourinsing matter, Ca-oxalate, glucose, carbohydrate and tartaric acid. The structure of three novel pregnane glycosides viz., Reticulin (1), Deniculatin (2) and Leptaculatin (3) isolate.

Amino Acids: Leptadenia reticulata shows the presence of 13 amino acids out of which some are essential amino acids. Leucine, valine, glutamic isoleucine, methionine, Phenyl alanine, tryptophan, arginine, alanine, tyrosine and proline.

Flavonoids: O-glycosides revealed the presence of diosmetin, apigening, acacetin, dimethoxy luleolin and Chrysoeriol. C-glycoside: Acacelin.

Phonetic Acid: Vanillic acid, syringic acid, p-coumaric acid (cis and trans isomers) and ferulic acid (cis and trans isomers), p-hydroxy benzoic acid, melilotic acid.
Lactogenic activity: Leptadenia reticulata administered to five cows in the dose of 1.5gm/cow/b.i.d for 15 days produced an increase in milk yield in four out of five cows with a net gain of 10.5% (Anjaria, 1980), Clinical study of Leptaden tablets (2 tablets 3 times a day) on 50 puerperal mothers showed a marked improvement in the flow of milk and fat content when compared to the control group mothers (Kasturi et al., 1980). In another study the powdered drug was administered at a dose of 1840 mg per day in cows and buffaloes, and produced a significant galactopoietic response. No significant changes were observed in the composition of milk or blood.
Other Medicinal Properties:
• Stimulates, Restores and Rejuvenates: Commonly, given for those suffering from weak debility or a lack of energy. It gives general strength to the body. It promotes health and vigour and alleviates the three dosas vata, pitta and kapha.
• Improves the digestive process and enhances metabolic activity.
• Effectively used in the treatment of habitual abortion and prevents abortion.
• Used in skin infections for ringworm, wounds, nose and ear disorders and asthma. It acts as antibacterial & anti-fungal agent. It also cures eye diseases, vomiting blood, emaciation, cough, dyspnoea (condition of painful or difficult breathing), fever & burning sensation.

ASPARAGUS RACEMOSUS (SHATAVARI):
Asparagus Racemosus Chemical composition and key active Nutritive Constituents:

Shatavari is rich in active constituents such as galactose, arabinose, steroidal glycosides and saponins which acts as a direct precursor for lactose production.

It contains steroidal saponins which are also called shatavarins 1-4. Shatwari 1 is the main glycoside which has 3 glucose & rhamnose moiteis attached to sarsasepogenin. Shatwari 4 on the other hand has proteins, alkaloids, tannin, starch and musilage. It also has Isoflavones which includes 8 methoxy 5,6,4 trihydroxyisoflavone 7-0-beta D glucopyranoside. It contains asparagamine, a polycyclic alkaloid and Racemosol which is a cyclic hydrocarbon. The roots contain polysaccharides. It is very low in sodium. It is a good source of folic acid, potassium, dietary fiber, and rutin.

Asparagus racemosus Lactogenic Activity: Five hundred grams of fresh roots of Asparagus racemosus per day, from 21st day after calving till 50 days was fed to lactating buffalos, which resulted in a significant increase in milk yield (Patil and Kanitkar, 1969). Lahar et al., (1991) demonstrated that Asparagus racemosus roots is rich source of minerals and trace elements such as calcium, copper, magnesium, iron, manganese, nickel, and zinc. Presence of calcium in the powder correlates with the analytical results for Galactogogue

The effects of the crude alcoholic extract of Asparagus racemosus 250mg/kg, intramuscularly were studied in post-partum, estrogen-primed and non-primed rats. The extract increased the weight of mammary glands in post-partum and estrogen-primed rats and uterine weight in estrogen-primed group. Increase in the weights of adrenals coupled with depletion of ascorbic acid suggests the release of pituitary adrenocorticotrophic hormone. The growth of the lobulo-alveolar tissue and milk secretion in the estrogen-primed rats may be due to the action of released corticoids or prolactin (Sabnis et al., 1968).

Asparagus racemosus root is the most commonly used traditional medicine in human
beings and its supplementation is recommended during last trimester of pregnancy to first
trimester after birth to the mother to boost milk quality and quantity, immunity of both
mother and fetus and to tone the reproductive system and reproductive health.

It has served as a potential management tool to improve lactation/milk production and
reproductive performance in crossbred dairy cows.

Asparagus racemosus recommended as a galactogogue in case of lactational inadequacy and can improve the milk production and reproduction capacity of dairy animals.

The use of Asparagus racemosus can also boost the immune system and consequently prevent the infection of the udder and reproductive organs of cows.

It can also be effectively used to reduce the stress of dairy animals and improve their productivity thus producing clean and healthy milk from them.

Specifically for females (Human & other Mammals):

Shatavari used in India as a best-known and most often-used herb for females. It is a general tonic and also a female reproductive tonic. Shatavari is used as the main Ayurvedic rejuvenative tonic for females. Shatavari roots are used as a drug that acts on all tissues as a powerful anabolic.

• Shatavari supports reproductive health by toning and nourishing the female reproductive organs.
• Shatavari maintains healthy hormonal balance.
• Shatavari treats PMS symptoms by relieving pain and controlling blood loss during menstruation.
• Shatavari supports and increases production of breast milk for nursing mothers.
• Shatavari relieves menopausal symptoms such as hot flashes. By producing estrogens it makes up for low estrogen levels in women who are menopausal or have had hysterectomies or oophorectomies.
• Used in India as a best-known and most often-used herb for women.
• Shatavari supports normal function of the immune and digestive system.
• Shatavari also increases libido.

Other Medicinal Properties:
Shatavari is a medicinal plant used widely as reproductive system tonic, immunity builder, antioxidant. anti-stress, used for a variety of serious diseases as also impotency of both the sexes. It is good for eyes, muscles, reproductive organs, increases milk secretion and helps to regain vigour and vitality.

WITHANIA SOMNIFERA (ASHWAGANDHA);

Ashwagandha is also called as Indian Ginseng. According to Charaka Samhita, Sushrut and Chakradutt there is no other plant in Ayurveda, which can provide so much of strength. Chakradutt says that consuming it brings back the youth fullness. Regular consumption of Ashwagandha for one year removes the deformities of the body.

Chemical composition and key active Nutritive Constituents:

The methanol, hexane and diethyl ether extracts from both leaves and roots of ashwagandha were found. Alkaloid percentage in roots ranges from 0.13 to 0.31%, The roots of Withania somnifera are alterative, aphrodisiac, deobstruent, diuretic, narcotic, sedative and restorative in nature, The pharmacological activity of the root is attributed to the alkaloids and steroidals lactones. The total alkaloid content in the roots of Indian types has been reported to vary between 0.13 and 0.3, though much high yields (up to 4.3 percent) have been recorded elsewhere. Many bio-chemical heterogeneous alkaloids, including choline, tropanol, pseudotopanol, cuscokygrene, 3- tigioyloxytropana, isopelletierine and several other steroidal lactories. Twelve alkaloids, 35 withanolides and several sitoindosides have been isolated from the roots of the plant have been studied. A sitoindoside is a biologically active constituent known as withanolide containing a glucose molecule at carbon 27. Indian ginseng's pharmacological activity has been attributed to two main withanolides, withaferin A and withanolide D. Withaferin-A is therapeutically active withanolide reported to be present in leaves. In addition to alkaloids, the roots are reported to contain starch, reducing sugars, glycosides, dulcitol, withancil, an acid and a neutral compound. The amino acids reported from the roots include aspartic acid, glycine, tyrosine, alanine, glutamic acid and cysteine. Macro elements recorded: nitrogen, phosphorous, magnesium, sodium, potassium and calcium contain Sodium, potassium and calcium, nitrogen, phosphorous and magnesium
copper and zinc. Components of micronutrients: lron, manganese, copper and zinc.

Plant-Chemicals:

(+)- withanine, somniferine, somnine, somniferinine, withananine, anhydrine, methanol, hexane, diethyl ether, alkaloid (0.13-0.31%), choline, tropanol, pseudotopanol, cuscokygrene, 3-tigioyloxytropana, isopelletierine, withaferin-A, starch, glycosides, dulcitol, withancil, aspartic acid, glycine, tyrosine, alanine, glutamic acid, cysteine.

The biochemical composition of WS has been widely studied and researched. Some 35
compounds had been analyzed in the laboratory.

1. Alkaloids: About 13 alkaloids are known as Isopelletierine, Anaferine, Cuseohygrine, Anahygrine, Tropine etc.
2. Somniferin: It is a bitter alkaloid with some hypnotic activity.
3. Steroidal Lactones: Withanolides, Withanoferins.
4. Saponins: These are with an additional acyl group (Sitoindoside VII and VIII).
5. Withanolides With Carbon At 27th Position: Sitoindoside IX and X.
6. Others: resin, fat, coloring matters, a reducing sugar, phytosterol, Ipuranol and a mixture of saturated and unsaturated acids.

Other Medicinal Properties:

• Ashwagandha provides better physical fitness.
• Ashwagandha has anti-aging and revitalizing properties.
• Ashwagandha increases the iron content in the blood.
• Ashwagandha purifies the blood.
• Ashwagandha immunity booster.
• Ashwagandha relieves total stress and hypertension.
• Ashwagandha is a strong rejuvenative.
• Ashwagandha is used for arthritic pain relief.
• Ashwagandha is great anti-inflammatory.
• Ashwagandha promotes sound sleep.
• Ashwagandha reduces high cholesterol levels.
• Ashwagandha enhances overall brain functions.

IPOMEA MAURITIANA (KSHEERAVIDARI, VIDARI, BHOOMIMANDA, PAYASVANI, IKSHUVALLI)

Chemical composition and key active Nutritive Constituents:
In India the root has been used from ancient Sanskritic times. It is considered tonic, alterative, aphrodisiac, demulcent, galactogogic and cholagogic. It is recommended for emaciation in children, and is put into a compound decoction which is nutritive, diuretic, expectorant and useful in fevers and bronchitis, in disease of the spleen and menorrhagia (5, 11). The root of Indian material has been reported to contain 1.3% content of a fixed oil consisting of glycerides of oleic (60%), linoleic (19%), palmitic (8%) and linolenic (1%) acids, beta sitosterol, glycoside, reducing sugars and mucilage. A resin resembling jalap resins is present. Ether soluble substances which are hypotensive and relaxant have been obtained; also, a non-ether soluble substance which raises blood pressure. increases respiration and stimulates smooth muscle and the uterus. A heterosidal substance, paniculatine, which acts in the manner of the latter has been isolated (8). Taraxerol, taraxerol acetate. umbelliferone, beta-sitosterol. scopoletin and 7-0-b-D-glycopyranosyl scopoletin (Scopolin). It contains a resin, similar to jalap resin, and is considered tonic, alterative, aphrodisiac, demulcent, lactagogue and cholagogue.

Medicinal Properties & common uses:
The roots are sweet, sour, acrid, astringent, cooling, emollient, aphrodisiac, galactagogue, rejuvenating, diuretic, depurative, stimulatn, appretiser, carminative, stomachic, anthelmintic, espectorant, and tonic. It contains a resin. similar to jalap resin. and is considered tonic, alterative. aphrodisiac. demulcent, lactagogue and cholagogue. The large tuberous root is regarded in local medicine as Tonic, alternative, aphrodisiac, demulcent and lactagogue. The powdered root stock is given for the purpose of increasing secretion of milk. The leaves enrich the blood (Yunani).

The drug promotes strength and complexion. improves voice. promotes breast milk and semen, overcomes vitiated pitta and vata and cures burning sensation. The drug is recommended in all cases of general debility and rheumatism. Its important preparations are drug Vidarayadi ghrtam. Dasmularishtam, Cyavanaprasam etc.

Intake of the plant root extracts causes increase in milk secretion. This is due to the presence of a certain glycoside resin. On entering the blood stream of the animal. They are as precursors for fatty acids. As this level increases, the precursors for vitamins increase subsequently. Thus, they help in the improvement of the milk quality and quantity as well.

They are useful in vitiated conditions of pitta and vata agaalactia, emaciation, strangury, leprosy, skin diseases, anorexia, dyspepsia, colic, flatulence, helminthiasis, bronchitis, fever, burning sensation, nausea, vomiting, pharyngodynia, syphilis, gonorrhea, inflammation, splenophathy, hepatopathy, menorrhagia and general debiliiy.

CITRUS AURANTIFOLIA (BRIHAT NIMBUKA. BRIHAT JAMBIRA):
Chemical composition and key active Nutritive Constituents:
Limonin (90%), (Z) Citral & (C) Citral (over3.5%), Flavonone Glycosides, Eriocitrin, Hesperidins, Ascorbic, citric, Malic, acids and their salts, essential oils. Vitamin A IU, Vitamin A RE, A - carotenoid, A - beta carotene, Vitamin C(high), folate, calcium. magnesium, phosphorus. Potassium (very high).
• Bio-Flavanoid extracts of Citrus limes are excellent for nervous system and blood flow, because of which the Feed Conversion ratio is very much improved.
• This plant is a proven remedy against cancers of all types. Some say it is very useful in all variants of cancer.
• It is considered also as an anti-microbial spectrum against bacterial infections and fungi.
• Effective against internal parasites and worms has a ‘Deworming’, effect.
• It regulates blood pressure which is too high and an antidepressant combats stress and nervous disorders.
• Useful for: vitiated pitta, kapha, cough, dyspepsia, nausea, flatulence, colic and anaemia.
, Claims:1. A method of preparing a poly herbal and poly nutrient formulated supplement for lactating animals, comprising the steps of;

- Processing and blending, laptadenia reticulate, asparagus racemosus, withania somnifera, ipomea mauritiana, citrus aurantifolia, hemidesmus indicus, galactogogue, lactogenic and anabolic.

2. The method of preparing a poly herbal and poly nutrient formulated supplement for lactating animals as claimed in claim 1 wherein, laptadenia reticulate is poly herbal formulation, comprises of phytoconstituents of plants, alpha-amyrin, beta-amyrin, ferulic acid, luteolin, desometin, rutin, beta-setosterol, stigmasterol, hentriacontanol, a triterpene alcohol simiarenol, apigenin, pregnane glycosides reticulin, deniculatin, leptaculatin,, acetyl alcohol, lupanol 3-O diglucoside, leptidine 1, saponins, flavonoids, luteolin, diosmtin, tannin, amino acids, phonetic acids, leaves with two resins, a bitter neutral principle, albuminous, colurinsing matter, co-oxalate, glucose, carbohydrate and tartaric acid.

3. The method of preparing a poly herbal and poly nutrient formulated supplement for lactating animals as claimed in claim 1 wherein, laptadenia reticulate shows the presence of 13 amino acids, out of which essential amino acids are leucine, valine, glutamic isoleucine, methionine, phenyl alanine, tryptophan, arginine, alanine, tyrosine and proline.

4. The method as claimed in claim 2 wherein, flavonoids reveal the presence of diosmetin, apigening, acacetin, dimethoxy luteolin, chrysoeriol.

5. The method as claimed in claim 2 wherein, photenic acids are vanillic acid, syringic acid, p-coumaric acid, ferulic acid, p-hydroxy benzoic acid and melilotic acid.

6. The method of preparing a poly herbal and poly nutrient formulated supplement for lactating animals as claimed in claim 1 wherein, asparagus racemosus is a species of asparagus plant comprises of steroidal saponins, isoflavones, asparagamine, a polycyclic alkaloid, racemosol, polysaccharides.

7. The method as claimed in claim 6 wherein, steroidal saponins comprises of shatwari 1 as main glucose which has 3 glucose and rhamnose moiteis attached to sarsasepogenin and shatwari 4 comprising proteins, alkaloids, tannin, starch and mucilage.

8. The method of preparing a poly herbal and poly nutrient formulated supplement for lactating animals as claimed in claim 1 wherein, the ipomea mauritiana comprises glycerides of oleic (60%), linoleic (19%), palmitic (8%) and linolenic (1%), beta-sitosterol, glycoside, reducing sugars mucilage, jalap resins, ether soluble substances, a non-ether soluble substance, a heterosidal substance, paniculatine, taraxerol, taraxerol acetate, umbelliferone, scopoletin, 7-O-B-D-glycopyranosyl scopoletin.