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A Method Of Purifying Human Growth Hormone And Purified Growth Hormone Thereof
Abstract: ABSTRACT The present invention relates to a method of purifying human growth hormone from a mixture containing at least one related impurity; and a purified growth hormone with purity of at least 85%.
Notices, Deadlines & Correspondence
Patent Information
Applicants
BIOCON LIMITED
Inventors
1. SHAIKH AZIMODDIN MINYASAB
2. SAGAR PRABHAKAR DHAMANE
3. PARTHA HAZRA
4. HARISH IYER
Specification
FIELD OF THE INVENTION
The invention provides for a method for purifying a desired heterologous polypeptide resulting from methylotrophic yeast fermentation broth. Specifically, the present invention relates to a process for the purification of a human growth hormone. More particularly the instant invention employs a chaotrope and a detergent to purify the desired protein from pigment related contaminants generated during the methanol induction phase of the fermentation. The invention relates to a simplified and optimized purification process of a polypeptide particularly Human growth hormone from a composition comprising the said polypeptide and at least one related pigment impurity by chromatographic procedures enabling high yields, selectivity and purity of the desired end product.
Documents
Application Documents
| # | Name | Date |
|---|---|---|
| 1 | 1183-che-2009 form-3 -30-07-2009.pdf | 2009-07-30 |
| 1 | 1183-CHE-2009_EXAMREPORT.pdf | 2016-07-02 |
| 2 | 1183-che-2009 correspondance others -30-07-2009.pdf | 2009-07-30 |
| 2 | 1183-CHE-2009 CORRESPONDENCE OTHERS 13-08-2012.pdf | 2012-08-13 |
| 3 | Form-5.pdf | 2011-09-03 |
| 3 | 1183-CHE-2009 FORM-1 13-08-2012.pdf | 2012-08-13 |
| 4 | Form-3.pdf | 2011-09-03 |
| 4 | 1183-CHE-2009 FORM-13 13-08-2012.pdf | 2012-08-13 |
| 5 | 1183-CHE-2009 CORRESPONDENCE OTHERS 05-12-2011.pdf | 2011-12-05 |
| 5 | Form-1.pdf | 2011-09-03 |
| 6 | 1183-CHE-2009 FORM-3 05-12-2011.pdf | 2011-12-05 |
| 6 | Drawings.pdf | 2011-09-03 |
| 7 | 1183-CHE-2009 FORM-3 05-12-2011.pdf | 2011-12-05 |
| 7 | Drawings.pdf | 2011-09-03 |
| 8 | 1183-CHE-2009 CORRESPONDENCE OTHERS 05-12-2011.pdf | 2011-12-05 |
| 8 | Form-1.pdf | 2011-09-03 |
| 9 | 1183-CHE-2009 FORM-13 13-08-2012.pdf | 2012-08-13 |
| 9 | Form-3.pdf | 2011-09-03 |
| 10 | Form-5.pdf | 2011-09-03 |
| 10 | 1183-CHE-2009 FORM-1 13-08-2012.pdf | 2012-08-13 |
| 11 | 1183-che-2009 correspondance others -30-07-2009.pdf | 2009-07-30 |
| 11 | 1183-CHE-2009 CORRESPONDENCE OTHERS 13-08-2012.pdf | 2012-08-13 |
| 12 | 1183-CHE-2009_EXAMREPORT.pdf | 2016-07-02 |
| 12 | 1183-che-2009 form-3 -30-07-2009.pdf | 2009-07-30 |