Claims:We Claim:
1. A method for rapid induction of lipid in fungi, said method comprising exposing the fungi to infrared radiation (IR), wherein increased content of lipid is obtained.
2. The method as claimed in claim 1, wherein the fungi is selected from a group comprising unicellular fungi, multicellular fungi and a combination thereof.
3. The method as claimed in claim 2, wherein the multicellular fungi is selected from a group comprising Aspergillus sp., Mucor sp, Chrysosporium sp., Fusarium sp., Scedosporium sp., Scytalidium sp., Scopulariopsis sp., Penicillium sp., Cladosporium sp.
4. The method as claimed in claim 2, wherein the unicellular fungi is yeast.
5. The method as claimed in claim 4, wherein the yeast is selected from a group comprising Saccharomyces sp., Candida sp and a combination thereof.
6. The method as claimed in claim 1, wherein the fungi is exposed to infrared radiation of a wavelength ranging from about 700 nm to 1200 nm, preferably about 1000 nm to 1100 nm.
7. The method as claimed in claim 6, wherein the fungi is exposed to infrared radiation at a wavelength of about 1100 nm.
8. The method as claimed in claim 1, wherein the fungi is exposed to infrared radiation for a time-period ranging from about 1 minute to 60 minutes.
9. The method as claimed in claim 8, wherein the fungi is exposed to infrared radiation for a time-period ranging from about 5 minutes to 30 minutes, preferably for about from 5 minutes to about 15 minutes.
10. The method as claimed in claim 1, wherein distance between the infrared radiation source and the fungi is ranging from about 1 cm to 100 cm.
11. The method as claimed in claim 10, wherein the distance between the infrared radiation source and the fungi is ranging from about 10 cm to 30 cm.
12. The method as claimed in any of the preceding claims, wherein the exposure is carried out in absence of light other than infrared radiation to obtain irradiated fungi.
13. The method as claimed in any of the preceding claims, wherein said method is carried out at a temperature ranging from about 30 °C to 35 °C for rapid induction of lipid.
14. The method as claimed in any of the preceding claims, wherein the method for rapid induction of lipid in the fungi, comprises steps of:
a. exposing the fungi to infrared radiation to obtain irradiated fungi; and
b. harvesting the irradiated fungi, followed by extracting lipid from the irradiated fungi, wherein increased lipid content is obtained.
15. The method as claimed in any of the preceding claims, wherein said method enhances neutral lipid yield in the fungi compared to the neutral lipid yield when fungi is not exposed to infrared radiation.
16. The method as claimed in any of the preceding claims, wherein the enhancement of neutral lipid yield is about 02 % to ¬¬¬¬¬80 %.
17. The method as claimed in any of the preceding claims, wherein said method enhances total lipid content in the fungi compared to the total lipid yield when fungi is not exposed to infrared radiation.
18. The method as claimed in any of the preceding claims, wherein the enhancement of total lipid yield is about 01 % to 12 %.

, Description:TECHNICAL FIELD
[001]. The present disclosure relates to the field of lipid enhancement in fungi. Particularly, the present disclosure relates to a method of rapid induction of lipids in fungi to obtain increased lipid content. In an exemplary embodiment, the present disclosure relates to a method of rapid induction of lipid in fungi by exposing the fungi to infrared radiation.

BACKGROUND
[002]. In the present scenario, the energy from renewable sources are interesting as they are the main raw sources for replacing the limited supply of fossil fuels.
[003]. There have been intense research efforts aimed at increasing and modifying the accumulation of lipids in organisms, such as fungi-through physical, biochemical and genetic approaches. However, the available approaches possess drawbacks, for instance, the biochemical approach causes reduced replication of the fungi, the physical approach suffers from limitations including lack of penetration and heat energy and the genetic approach is not cost effective and is cumbersome. Thus, the available approaches are complicated and suffer from various drawbacks.
[004]. Therefore, there is a need to develop simple, cost-effective and more efficient process for rapid induction of lipids in organisms, such as fungi. The present disclosure addresses said need.

SUMMARY OF THE DISCLOSURE
[005]. The present disclosure provides for simple, efficient and cost-effective method of rapid induction of lipids in fungi.
[006]. In an embodiment, the present disclosure relates to a method of rapid induction of lipids in fungi.
[007]. In an embodiment, the present disclosure relates to a method of rapid induction of neutral lipid and total lipid content, independently in fungi.
[008]. In an embodiment of the present disclosure, the method of rapid induction of lipids comprises exposing fungi to infrared radiation.
[009]. In an embodiment of the present disclosure, the fungi is exposed to infrared radiation for a time-period ranging from about 1 minute to 60 minutes.
[0010]. In an embodiment of the present disclosure, the distance between the infrared radiation source and the fungi is ranging from about 1 cm to 100 cm.
[0011]. In an embodiment of the present disclosure, the combination of distance between the infrared radiation source and the fungi and the duration of exposure of the fungi to infrared radiation is an essential feature in the present method for rapid induction of lipid in the fungi to obtain increased lipid content.

BRIEF DESCRIPTION OF THE ACCOMPANYING FIGURES
[0012]. In order that the present disclosure may be readily understood and put into practical effect, reference will now be made to exemplary embodiments as illustrated with reference to the accompanying figures. The figures together with detailed description below, are incorporated in and form part of the specification, and serve to further illustrate the embodiments and explain various principles and advantages, where:

[0013]. Figure 1 depicts the exemplary experimental step of the method of the present disclosure.
[0014]. Figure 2 depicts the image of Aspergillus awamori obtained from optical microscope.
[0015]. Figure 3 depicts the lipid profile of Aspergillus awamori.
[0016]. Figure 4 depicts the image of Mucor hiemalis obtained from TEM.
[0017]. Figure 5 depicts the image of Mucor hiemalis obtained from optical microscope.
[0018]. Figure 6 depicts the lipid profile of Mucor hiemalis.
[0019]. Figure 7 depicts the image of Saccharomyces cerevisiae obtained from TEM.
[0020]. Figure 8 depicts the lipid profile of Saccharomyces cerevisiae.
[0021]. Figure 9 depicts the image of Candida albicans obtained from TEM.
[0022]. Figure 10 depicts the lipid profile of Candida albicans.

DESCRIPTION OF THE DISCLOSURE
[0023]. To address the limitations as stated in the background, the present disclosure provides a simple and efficient method for rapid induction of lipid in fungi to obtain increased lipid content.
[0024]. In an embodiment, the present disclosure relates to a method of rapid induction of neutral lipid in fungi.
[0025]. As used herein, ‘neutral lipids’ refer to hydrophobic molecules (lipid moieties) lacking charged groups. Triacylglycerols (TAGs), steryl esters (SEs) and wax esters (WEs) majorly form the group of neutral lipids.
[0026]. In another embodiment, the present disclosure relates to a method of rapid induction of total lipid in fungi.
[0027]. As used herein, ‘total lipids’ consists of neutral lipids and polar lipids.
[0028]. In an embodiment, the present disclosure relates to a method of rapid induction of lipid in fungi by exposing the fungi to infrared radiation (IR) at a predetermined distance, for a predetermined duration, to obtain increased lipid content.
[0029]. In an embodiment of the present disclosure, the fungi are selected from a group comprising unicellular fungi, multicellular fungi and a combination thereof.
[0030]. In an embodiment of the present disclosure, the unicellular fungi are yeast.
[0031]. In an embodiment of the present disclosure, the multicellular fungi is selected from a group comprising Aspergillus sp., Mucor sp, Chrysosporium sp., Fusarium sp., Scedosporium sp., Scytalidium sp., Scopulariopsis sp., Penicillium sp., Cladosporium sp,.
[0032]. In an embodiment of the present disclosure, the yeast is selected from a group comprising Saccharomyces sp., Candida sp. and any combination fungal sp., thereof.
[0033]. In an exemplary embodiment of the present disclosure, the fungi are selected from a group comprising Aspergillus awamori Mucor hiemalis, Saccharomyces cerevisiae and Candida albicans.
[0034]. In an embodiment of the present disclosure, the method comprises exposing the fungi to infrared radiation at a wavelength ranging from about 700 nm to 1200 nm for rapid induction of lipid in the fungi.
[0035]. In a preferred embodiment of the present disclosure, the fungi are exposed to infrared radiation at a wavelength ranging from about 1000 nm to 1100 nm.
[0036]. In a more preferred embodiment of the present disclosure, the fungi are exposed to infrared radiation at a wavelength of about 1100 nm.
[0037]. In an embodiment of the present disclosure, the fungi are exposed to infrared radiation for a duration ranging from about 1 minute to 60 minutes.
[0038]. In a preferred embodiment of the present disclosure, the fungi are exposed to infrared radiation for a duration ranging from about 1 minute to 30 minutes.
[0039]. In a more preferred embodiment of the present disclosure, the fungi are exposed to infrared radiation for a duration ranging from about 1 minute to 20 minutes.
[0040]. In another preferred embodiment of the present disclosure, the fungi are exposed to infrared radiation for a duration ranging from about 5 minutes to 20 minutes.
[0041]. In an exemplary embodiment of the present disclosure, the fungi are exposed to infrared radiation for a duration of about 1 minute, about 2 minutes, about 3 minutes, about 4 minutes, about 5 minutes, about 6 minutes, about 7 minutes, about 8minutes, about 9 minutes, about 10minutes, about 11minutes, about 12 minutes, about 13 minutes, about 14 minutes, about 15 minutes, about 16 minutes, about 17 minutes, about 18minutes, about 19minutes or about 20minutes.
[0042]. In an embodiment of the present disclosure, the distance between the infrared radiation source and the fungi is ranging from about 1 cm to 100 cm.
[0043]. In a preferred embodiment of the present disclosure, the distance between the infrared radiation source and the fungi is ranging from about 1 cm to 30 cm.
[0044]. In another preferred embodiment of the present disclosure, the distance between the infrared radiation source and the fungi is ranging from about 10 cm to 30 cm.
[0045]. In an exemplary embodiment of the present disclosure, the distance between the infrared radiation source and the fungi is about 10cm, about 15cm, about 20cm, about 25cm, about 30cm, about 35cm, about 40cm, about 45cm, about 50cm, about 55cm, about 60cm, about 65cm, about 70cm, about 75cm, about 80cm, about 85cm, about 90cm, about 95cm or about 100cm.
[0046]. In an embodiment of the present disclosure, the fungi are exposed to infrared radiation in absence of light other than infrared radiation to enable rapid induction of lipid in the fungi.
[0047]. In an embodiment of the present disclosure, the method is carried out in presence or absence of carbon dioxide (CO2).
[0048]. In an embodiment of the present disclosure, the CO2 if present, has a concentration ranging from about 0 % to10 %.
[0049]. In an embodiment of the present disclosure, the method is carried out at a temperature ranging from about 30 °C to 35 °C for rapid induction of lipid in the fungi.
[0050]. In an exemplary embodiment of the present disclosure the method is carried out at a temperature of about 30 °C, about 31 °C, about 32 °C, about 33 °C, about 34 °C or about 35 °C for rapid induction of lipid in the fungi.
[0051]. In an embodiment of the present disclosure, the method of rapid induction of lipid in the fungi comprises steps of:
i. exposing the fungi to infrared radiation to obtain irradiated fungi;
ii. harvesting the irradiated fungi, followed by extracting lipid from the irradiated fungi, wherein increased lipid content is obtained.
[0052]. In another embodiment of the present disclosure, the method of rapid induction of lipid in the fungi comprises step of:
i. exposing the fungi to infrared radiation to obtain irradiated fungi;
ii. harvesting the irradiated fungi, followed by extracting lipid from the irradiated fungi, wherein increased lipid content is obtained.
[0053]. In another embodiment of the present disclosure, the method of rapid induction of lipid in the fungi comprises steps of:
(i) exposing the fungi to infrared radiation to obtain irradiated fungi;
(ii) harvesting the irradiated fungi;
(iii) drying the harvested fungi to obtain powdered fungi;
(iv) extracting the powdered fungi with a solvent to extract the lipid, wherein increased lipid content is obtained.
[0054]. In an embodiment, the above described steps of exposing, harvesting, drying, and extracting comprises techniques selected from shaking, sonication, centrifugation, heating, grinding, drying, stirring, decoupling of heat using a liquid medium and any combination thereof.
[0055]. In an embodiment, the present method enhances lipid yield in the fungi by about 02 % to ¬¬¬¬80 % compared to the lipid yield when the fungi are not exposed to infrared radiation.
[0056]. In another embodiment, the present method enhances lipid yield in the fungi by about 2%, about 4%, about 6%, about 8%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%., about 75% or about 80% compared to the lipid yield when the fungi are not exposed to infrared radiation.
[0057]. In an embodiment, the present method enhances lipid yield in the fungi by about 02 % to ¬¬¬¬80 % compared to the lipid yield when the fungi is not exposed to infrared radiation.
[0058]. In another embodiment, the present method enhances lipid yield in the fungi by about 2%, about 4%, about 6%, about 8%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%., about 75% or about 80% compared to the lipid yield when the fungi is not exposed to infrared radiation.
[0059]. In an embodiment, the present method enhances neutral lipid yield in the fungi by about 2% to 80% compared to the neutral lipid yield when the fungi is not exposed to infrared radiation.
[0060]. In another embodiment, the present method enhanced neutral lipid yield in the fungi by about 2%, about 4%, about 6%, about 8%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%., about 75% or about 80% compared to the neutral lipid yield when the fungi is not exposed to infrared radiation.
[0061]. In an embodiment, the present method enhances total lipid yield in the fungi by about 1% to 12% compared to the total lipid yield when the fungi is not exposed to infrared radiation.
[0062]. In another embodiment, the present method enhances total lipid yield in the fungi by about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 11% or about 12% in the fungi compared to the total lipid yield when the fungi is not exposed to infrared radiation.
[0063]. In an exemplary embodiment of the present disclosure, the method of rapid induction of lipid in the fungi comprises steps of:
(i) exposing the fungi to infrared radiation at a wavelength of about 700 nm to 1200 nm for a duration ranging from about 1minute to 60 minutes to obtain irradiated fungi, wherein the distance between the infrared radiation source and the fungi is about 1 cm to 100 cm; and
(ii) harvesting the irradiated fungi, followed by extracting lipid from the irradiated fungi, wherein increased lipid content is obtained
[0064]. In another exemplary embodiment of the present disclosure, the method of rapid induction of lipid in the fungi comprises steps of:
(i) exposing the fungi to infrared radiation at a wavelength of about 1000 nm to 1100 nm for a duration ranging from about 1 minute to 30 minutes to obtain irradiated fungi, wherein the distance between the infrared radiation source and the fungi is about 1 cm to 30 cm; and
(ii) harvesting the irradiated fungi, followed by extracting lipid from the irradiated fungi, wherein increased lipid content is obtained.
[0065]. In another exemplary embodiment of the present disclosure, the method of rapid induction of lipid in the fungi comprises steps of:
(i) exposing the fungi to infrared radiation at a wavelength of about 1100 nm for a duration 5 minutes to obtain irradiated fungi, wherein the distance between the infrared radiation source and the fungi is about 15cm; and
(ii) harvesting the irradiated fungi, followed by extracting lipid from the irradiated fungi, wherein increased lipid content is obtained.
[0066]. In another exemplary embodiment of the present disclosure, the method of rapid induction of lipid in the fungi comprises steps of:
(i) exposing the fungi to infrared radiation at a wavelength of about 700 nm to 1200 nm for a duration ranging from about 1minute to 60 minutes to obtain irradiated fungi, wherein the distance between the infrared radiation source and the fungi is about 1 cm to 100 cm;
(ii) harvesting the irradiated fungi;
(iii) drying and the harvested fungi to obtain powdered fungi; and
(iv) extracting the powdered fungi with a solvent, wherein increased lipid content is obtained.
[0067]. In an embodiment of the present disclosure, harvesting the irradiated fungi is carried out by centrifugation, flocculation, coagulation, electrocoagulation, filtration and any combination thereof.
[0068]. In another embodiment of the present disclosure, drying the harvested fungi is carried out by oven/sunlight drying, lyophilization/freeze drying and any combination thereof.
[0069]. In yet another embodiment of the present disclosure, extracting the powdered fungi is carried out by solvent extraction with or without sonication. The solvent employed is selected from a group comprising chloroform, methanol, toluene, hexane, isopropanol and any combination thereof.
[0070]. In an embodiment, the method of the present disclosure causes production of Eicosapentaenoic acid (EPA) and/or Docosahexaenoic acid (DHA) in the fungi only upon exposure of the fungi to infrared radiation at predetermined distance, for a predetermined duration, as described above. However, naturally (without exposure to infrared radiation), the fungi does not produce EPA and/or DHA.
[0071]. In an embodiment, in the method of the present disclosure, the infrared radiations penetrates the fungi and vibrates at molecular level and induces oxidative stress which eventually leads to rapid induction of lipid, thereby causing increased lipid content from the fungi.
[0072]. Additional embodiments and features of the present disclosure will be apparent to one of ordinary skill in art based upon description provided herein. The embodiments herein provide various features and advantageous details thereof in the description. Descriptions of well-known/conventional methods and techniques are omitted so as to not unnecessarily obscure the embodiments herein. Further, the disclosure herein provides for examples illustrating the above described embodiments, and in order to illustrate the embodiments of the present disclosure certain aspects have been employed. The examples used herein for such illustration are intended merely to facilitate an understanding of ways in which the embodiments herein may be practiced and to further enable those of skill in the art to practice the embodiments herein. Accordingly, the following examples should not be construed as limiting the scope of the embodiments herein.

EXAMPLES
[0073]. The following fungal strains have been employed in the Examples:
Accession numbers of the fungal strains are provided below-
Multicellular Fungus/mycelial cells
1. Aspergillus awamori Nakazawa. ATCC No: 11382
2. Mucor hiemalis Wehmer. NCIM No: 873

Unicellular fungus/Non-mycelial cells
1. Saccharomyces cerevisiae Hansen. NCIM No.3044
2. Candida albicans (Robin) Berkhout. ATCC No: 18804
EXAMPLE 1:
The fungal strains, such as Aspergillus awamori Nakazawa. ATCC No.: 11382Mucor hiemalis Wehmer. NCIM No: 873, Saccharomyces cerevisiae Hansen. NCIM No.3044 and Candida albicans (Robin) Berkhout.ATCC No: 18804, were grown independently in a Sabouraud’s broth media at a temperature of about in 30ºC for a duration of about 72 hours. The Fungal strains were exposed to Infrared radiation (1100 nm, shortwave IR-A, 240V, 150W Philips) for a duration of about 5minutes, about 10minutes, about 20minutes, respectively. The distance between the infrared radiation source and the fungal strains was 15cm, 30cm and 100cm, respectively.
The Table 1 illustrates the combination chart of tested physical parameters (distance and time combination chart).
Rapid induction of lipid in the fungal strains were assessed by analyzing total lipid and neutral lipid content by gravimetric extraction, analyzing fatty acid methyl esters (FAME) composition by Gas chromatography (GC) and by microscopy (Optical and TEM), with respect to control samples (fungal strains not exposed to infrared radiation).
Table 1: Combination chart of tested physical parameters (distance and time combination chart)
Sr. N Strain IR lamp distance from the top of culture (cm) Minutes of exposure
(minutes)
1 Aspergillus awamori 15 5, 10 & 20, independently
30 5, 10 & 20, independently
100 5, 10 & 20, independently
2 Saccharomyces cerevisiae 15 5, 10 & 20, independently
30 5, 10 & 20, independently
100 5, 10 & 20, independently
3 Candida albicans 15 5
4 Mucor hiemalis 15 5

Results:
1. Lipid data through gravimetric analysis:
Under IR exposure conditions (according to Table 1) all the strains showed doubled lipid enhancement compared to control. A unique correlation between distance and exposure time. For instance, similar amount of lipid content in 15 cm distance and 5minute exposure time and 100cm distance and 20minutes exposure time was observed (see figure 3). However, in the algal strains, when the distance between the algal strain and IR source and the IR exposure time was increased lipid content decreased.
• Figure 3 illustrates the lipid profile of Aspergillus awamori;
• Figure 6 illustrates the lipid profile of Mucor hiemalis;
• Figure 8 illustrates the lipid profile of Saccharomyces cerevisiae; and
• Figure 9 illustrates the lipid profile of Candida albicans.

2. TEM images/optical microscopy analysis:
Microscopic analysis by Transmission electron microscopy (TEM) or optical microscopy showed clear black stained lipid spots inside the cells than the control (see figures 2, 4, 5, 7 and 9)

3. Gas chromatography analysis:
i. For Aspergillus awamori
Palmitic acid (C16:0), stearic acid (C18:0), Heneicosylliic acid (C21:0) EPA (C20:5n3) were detected under IR treated culture when compared to control.

ii. For Saccharomyces cerevisiae
Palmitoleic acid (C16:1), Gincgolic acid (C17:1), Paullinic acid (C20:1), Tricosyllic acid (C23:0), Arachidonic acid (C20:4n6), EPA (C20:5n3), DHA (C22:6n3) were detected under IR treated culture when compared to control.

iii. For Candida albicans
Lignoceric acid (C24:0) and EPA (C20:5n3) were detected under IR treated culture when compared to control.

Table 2 Illustrates the gas chromatography (GC) profile of all tested organisms under IR treated and untreated, showing variation in FAME profile.
According to the data in the Table 2 infrared radiation (IR) treated cultures of Candida albicans, Saccharomyces cerevisiae and Aspergillus awamori showed change in the FAME profile when compared to control cultures. It was observed that IR exposure caused induction of Cis-11, 14-Eicosapentaenoic acid (C20:5n3) compound in all the strains when compared to Control.

Strain Fatty acid FAME %
IR treated Control
Candida albicans Lignocerate (C24:0) 0.021 ND
Cis-11,14-Eicosapentaenoic acid (C20:5n3) 0.005 ND
Saccharomyces cerevisiae Methyl Palmitoleate (C16:1) 0.096 ND
Cis-10-Heptadecanoic Acid Methyl (C17:1) 0.145 ND
Methyl cis-11-Eicosanoate (C20:1) 0.400 0.76
Lignocerate (C24:0) 0.021 ND
Cis-11,14-Eicosapentaenoic acid (C20:5n3) 0.005 ND
Docosa Hexanoic Acid (C22:6n3) 0.141 ND
Aspergillus awamori Methyl Palmitate (C16:0) 0.200 ND
Methyl Stearate (C18:0) 0.059 ND
Methyl cis-11-Eicosanoate (C21:0) 0.109 ND
Cis-11,14-Eicosapentaenoic acid (C20:5n3) 0.005 ND
Table 2: FAME profile showing the components which are vital and economically viable. ND: Not Detected (below detectable limits)

ADVANTAGES
[0074]. The present disclosure enables enhanced lipid production in fungi by a simple and efficient method using IR exposure at specific combination of distance between the IR source, fungi and exposure time as described above.
[0075]. The present method increases the neutral lipid content and total lipid content in fungi at a shorter time duration, without reduction of biomass.
[0076]. The enhanced lipid content obtained by the present method is immensely useful in producing high quantities of biofuels.
[0077]. The present method is cost-effective in large scale cultivation.
[0078]. Additional embodiments and features of the present disclosure will be apparent to one of ordinary skill in art based on the description provided herein. The embodiments herein provide various features and advantageous details thereof in the description. Descriptions of well-known/conventional methods and techniques are omitted so as to not unnecessarily obscure the embodiments herein.
[0079]. The foregoing description of the specific embodiments fully reveals the general nature of the embodiments herein that others can, by applying current knowledge, readily modify and/or adapt for various applications such specific embodiments without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. Therefore, while the embodiments in this disclosure have been described in terms of preferred embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the embodiments as described herein.
[0080]. With respect to the use of substantially any plural and/or singular terms herein, those having skill in the art can translate from the plural to the singular and/or from the singular to the plural as is appropriate to the context and/or application. The various singular/plural permutations may be expressly set forth herein for sake of clarity.
[0081]. The use of the expression “at least” or “at least one” suggests the use of one or more elements or ingredients or quantities, as the use may be in the embodiment of the disclosure to achieve one or more of the desired objects or results.
[0082]. Any discussion of documents, acts, materials, devices, articles and the like that has been included in this specification is solely for the purpose of providing a context for the disclosure. It is not to be taken as an admission that any or all of these matters form a part of the prior art base or were common general knowledge in the field relevant to the disclosure as it existed anywhere before the priority date of this application.
[0083]. While considerable emphasis has been placed herein on the particular features of this disclosure, it will be appreciated that various modifications can be made, and that many changes can be made in the preferred embodiments without departing from the principles of the disclosure. These and other modifications in the nature of the disclosure or the preferred embodiments will be apparent to those skilled in the art from the disclosure herein, whereby it is to be distinctly understood that the foregoing descriptive matter is to be interpreted merely as illustrative of the disclosure and not as a limitation.