1. A microfluidic device (100) for inducing bidirectional oscillatory shear stress on biological cells (10), the microfluidic device (100) comprising: a coverslip (1), defined with a flow surface adapted to receive a plurality of biological cells (10); and a cover member (2) disposed on the coverslip (1), wherein the cover member (2) and the coverslip (1) define a chamber (6) for receiving fluid, the cover member (2) further comprises: a first inlet section (3) defined at a portion of the cover member (2), wherein the first inlet section (3) is configured to selectively receive and channelize fluid at a first predetermined velocity into the chamber (6); and a second inlet section (4) defined at a portion of the cover member (2) away from the first inlet section (3), wherein the second inlet section (4) is configured to selectively receive and channelize fluid at a second predetermined velocity into the chamber (6); wherein, fluid channelized at the first predetermined velocity and the second predetermined velocity into the chamber (6) creates a predefined oscillatory bi-directional flow pattern to induce predefined wall shear stress on the plurality of biological cells (10).

2. The microfluidic device (100) as claimed in claim 1, wherein the plurality of biological cells (10) are cultured on the coverslip (1).

3. The microfluidic device (100) as claimed in claim 1, comprises an outlet section (5) defined at a portion of the cover member (2) opposite to the first inlet section (3) and the second inlet section (4), the outlet section (5) is configured to dispense fluid out of the chamber (6).

4. The microfluidic device (100) as claimed in claim 3, wherein the outlet section (5) is fluidly coupled to a reservoir adapted to receive and store fluid from the chamber (6).

5. The microfluidic device (100) as claimed in claim 1, wherein the first inlet section (3), the second inlet section (4) and the outlet section (5) are defined in a spaced apart configuration with an angular separation.

6. The microfluidic device (100) as claimed in claim 1, wherein the coverslip (1) is made of a transparent material for microscopic observation of the plurality of biological cells (10).

7. The microfluidic device (100) as claimed in claim 1, wherein the coverslip (1) and the cover member (2) are adapted to be positioned within a frame configured to encompass the microfluidic device (100).

8. The microfluidic device (100) as claimed in claim 7, wherein the frame includes a first part defined with a cavity to receive the microfluidic device (100) and a second part adapted to be fixed on the first part to enclose the microfluidic device (100).

9. A microscope configured to selectively receive the microfluidic device (100), as claimed in claim 1, to observe reaction of a plurality of biological cells (10) to bi-directional oscillatory flow pattern.

We claim:
1. A microfluidic device (100) for inducing bidirectional oscillatory shear stress on biological
cells (10), the microfluidic device (100) comprising:
a coverslip (1), defined with a flow surface adapted to receive a plurality of biological cells (10); and
a cover member (2) disposed on the coverslip (1), wherein the cover member (2) and the coverslip (1) define a chamber (6) for receiving fluid, the cover member (2) further comprises:
a first inlet section (3) defined at a portion of the cover member (2), wherein
the first inlet section (3) is configured to selectively receive and channelize fluid at
a first predetermined velocity into the chamber (6); and
a second inlet section (4) defined at a portion of the cover member (2) away
from the first inlet section (3), wherein the second inlet section (4) is configured to
selectively receive and channelize fluid at a second predetermined velocity into the
chamber (6);
wherein, fluid channelized at the first predetermined velocity and the
second predetermined velocity into the chamber (6) creates a predefined oscillatory
bi-directional flow pattern to induce predefined wall shear stress on the plurality of
biological cells (10).
2. The microfluidic device (100) as claimed in claim 1, wherein the plurality of biological cells (10) are cultured on the coverslip (1).
3. The microfluidic device (100) as claimed in claim 1, comprises an outlet section (5) defined at a portion of the cover member (2) opposite to the first inlet section (3) and the second inlet section (4), the outlet section (5) is configured to dispense fluid out of the chamber (6).
4. The microfluidic device (100) as claimed in claim 3, wherein the outlet section (5) is fluidly coupled to a reservoir adapted to receive and store fluid from the chamber (6).

5. The microfluidic device (100) as claimed in claim 1, wherein the first inlet section (3), the second inlet section (4) and the outlet section (5) are defined in a spaced apart configuration with an angular separation.
6. The microfluidic device (100) as claimed in claim 1, wherein the coverslip (1) is made of a transparent material for microscopic observation of the plurality of biological cells (10).
7. The microfluidic device (100) as claimed in claim 1, wherein the coverslip (1) and the cover member (2) are adapted to be positioned within a frame configured to encompass the microfluidic device (100).
8. The microfluidic device (100) as claimed in claim 7, wherein the frame includes a first part defined with a cavity to receive the microfluidic device (100) and a second part adapted to be fixed on the first part to enclose the microfluidic device (100).
9. A microscope configured to selectively receive the microfluidic device (100), as claimed in claim 1, to observe reaction of a plurality of biological cells (10) to bi-directional oscillatory flow pattern.