ABSTRACT
876/CHENP/2004
"A mutated G-CSF mRNA or cDNA fragment or protein as a diagnostic
cancermarker''
This invention relates to a mutated G-CSF mRNA or cDNA fragment iiaving a deletion of an exon 3 region for use as a diagnostic cancermarker.

WE CLAIM:
1. A mutated G-CSF mRNA or cDNA fragment having a deletion of an exon 3 region for use as a diagnostic cancermarker.
2. A mutated G-CSF protein having a deletion of an amino acid sequence corresponding to an exon 3 region for use as a diagnostic cancermarker.
3. A microarray for diagnosis of cancer comprising (a) a DNA fragment corresponding to exon 3 of a G-CSF gene, and (b) at least one of DNA fragments corresponding to exons 1, 2, 4 and 5 of said G-CSF gene are bound.
4. A diagnostic agent for cancer comprising an antibody against a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to an exon 3 region of said G-CSF protein.
5. A microarray for diagnosis of cancer comprising an antibody against a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to an exon 3 region of said G-CSF protein.
6. A primer for use in amplification of a G-CSF gene in a G-CSF nucleic acid sample obtained from mammalian tissues or cells, wherein said primer is selected from the group consisting of the following nucleotide sequence pairs:
sense: 5'-ACCCCCCTGGGCCCTGCC-3' (SEQ ID NO. 1) and

antisense: 5'-TCAGGGCTGGGCAAGGTG-3' (SEQ ID NO. 2); sense: 5'-ACCCCCCTGGGCCCTGCC-3' (SEQ ID NO. 1) and antisense: 5'-CAGCTGCAGGGCCTGGCT-3' (SEQ ID NO 5); sense: 5'-ACCCCCCTGGGCCCTGCC-3' (SEQ ID NO. 1) and antisense: 5'-CGCTATGGAGTTGGCTCAAGC-3' (SEQ ID NO. 6); sense: 5'-ACCCCCCTGGGCCCTGCC-3' (SEQ ID NO. 1) and antisense: 5'-CAGCTTCTCCTGGAGCGC-3' (SEQ ID NO. 9); sense: 5'-ATCCAGGGCGATGGCGCAGCG-3' (SEQ ID N0.3) and antisense: 5'-TCAGGGCTGGGCAAGGTG-3' (SEQ ID NO. 2); sense: 5'-ATCCAGGGCGATGGCGCAGCG-3' (SEQ ID N0.3) and antisense: 5'-CAGCTGCAGGGCCTGGCT-3' (SEQ ID NO. 5); sense: 5'-ATCCAGGGCGATGGCGCAGCG-3' (SEQ ID N0.3) and antisense: 5'-CGCTATGGAGTTGGCTCAAGC-3' (SEQ ID NO. 6); sense: 5'-TGTGCCACCTACAAGCTGTGC-3' (SEQ ID N0.4) and antisense: 5'-TCAGGGCTGGGCAAGGTG-3' (SEQ ID NO. 2); sense: 5'-TGTGCCACCTACAAGCTGTGC.3' (SEQ ID N0.4) and antisense: 5'-CAGCTGCAGGGCCTGGCT-3' (SEQ ID NO. 5); and sense: 5'-TGTGCCACCTACAAGCTGTGC-3' (SEQ ID N0.4) and antisense: 5'-CGCTATGGAGTTGGCTCAAGC-3' (SEQ ID NO. 6).
7. A mutated G-CSF protein having a deletion of an amino acid sequence corresponding to an exon 3 region.

8. An antibody specifically binding to the mutated G-CSF protein as claimed in
claim 7.
9. The antibody as claimed in claim 8, wherein the antibody is a polyclonal
antibody.
10. The antibody as claimed in claim 8, wherein the antibody is a monoclonal
antibody.