DESC:FIELD OF THE INVENTION

[0001] The present disclosure generally relates to a nanoparticle composition. The present disclosure, in particular, relates to a nanocopper composition. Specifically, the present disclosure relates to a composition that aids to prevent and control infectious diseases, preferably coronavirus or norovirus. The present disclosure also relates to a method of preparing the nanoparticle composition.

BACKGROUND

[0002] Coronavirus disease 2019 or COVID-19 is an infectious disease which is spreading globally at an unprecedented rate. Experts are encouraging social distance and use of face masks in order to prevent further spread of the COVID-19 and other pathogenic diseases. Hygienic practices such as washing hands regularly will protect from many pathogenic diseases.

[0003] Typically, disinfectants utilize 60 to 95 percent of alcohol with combination of isopropanol, ethanol, and n-propanol. These alcohol-based sanitizers destroy cell wall of microbes or interferes with their metabolism thereby killing the microbes in the process. However, the alcohol-based hand sanitizers will kill both beneficial and non-beneficial microbes which causes various problems in humans. However, it is still not known, whether available hand-sanitizer are effective against killing viruses such as novel coronavirus.

[0004] Conventionally, alcohol-based hand sanitizers are used to wash hands before intake of any food products. This prevents the microbes to enter into the human body along with the food. However, alcohol will also be ingested along with the food products thereby resulting in alcohol poisoning in the human body. The endocrine disruptors which are available in the alcohol is a major factor for early puberty in children. The most common types of hostile health effects for both alcohol and non-alcohol-based hand sanitizers include ocular irritation, vomiting, conjunctivitis, oral infection, cough, abdominal pain, etc.

[0005] There are no preventive composition are available in the market that is effective against the deadly and fatal infectious diseases like coronavirus. Surprisingly, it was observed that the nanoparticles is a potential antiviral agent due to unique attributes of nanomaterials including intrinsic anti-viral property. However, there is no data or study available on any nanoparticle composition that can kill coronavirus or norovirus.

[0006] Therefore, there exists a need to provide a nanoparticle composition that requires less amount of alcohol and also aids in preventing and controlling infectious diseases, preferably coronavirus or norovirus and method of preparing the same.

OBJECT OF THE INVENTION

[0007] Some of the objects of the present disclosure, with at least one embodiment herein satisfy, are listed herein below:

[0008] It is one of the primary object of the present disclosure to provide a nanoparticle composition that aids to prevent and control infectious diseases, preferably coronavirus or norovirus.

[0009] It is one of the object of the present disclosure to provide a nanoparticle composition having minimal amount of alcohol.

[0010] It is another object of the present disclosure to provide a nanoparticle composition that is environmentally benign and easily available.

[0011] It is another object of the present disclosure to explore the antimicrobial or antiviral properties activities of nanocopper, which is safe and abundantly available.

[0012] It is another object of the present disclosure to provide an eco-friendly nanocopper composition, which is cost efficient.

[0013] It is another object of the present disclosure to provide safe and effective composition against air infection and harmless to human body.

[0014] It is another object of the present disclosure to provide a method of preparing a nanoparticle composition that kills coronavirus or norovirus.

SUMMARY OF THE INVENTION

[0015] The present disclosure discloses a nanoparticle composition comprising, by volume: deionized water in an amount having a range of 88%-98% of the total composition; metal nanoparticles in an amount having a range of 0.4%-0.6 % of the total composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm; one or more pH modifiers having an amount in the range of 0.2% - 0.6% of the total composition; one or more surfactant having an amount in the range of 0.1%-0.3% of the total composition; one or more humectant having an amount in the range of 0.2%- 0.4% of the total composition; and one or more essential oils having an amount in the range of 0.1% - 0.2% of the total composition.
[0016] The present disclosure also relates to a method of preparing a nanoparticle composition, comprising the steps of: mixing copper chloride dihydrate and sodium hydroxide in water for a time period of 55 mins-65 mins to form solution A; mixing one or more pH modifiers in deionized water to form solution B; and heating solution B for a time period of 50 mins-70 mins till the temperature reaches 55°C-65°C; mixing one or more surfactants and humectants in deionized water for a time period of 10 hours -12 hours to form solution C; mixing essential oils in the deionized water to form solution D for a time period of 25 mins-35 mins; and mixing the solutions A-D together to form the nanoparticles composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm.

BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The illustrated embodiments of the subject matter will be best understood by reference to the drawings, wherein the parts are designated with numerals throughout. The following description is intended only by way of example, and simply illustrates certain selected embodiments of compositions, devices, systems, and methods that are consistent with the subject matter as claimed herein, wherein:

[0018] Figure (Fig.) 1 illustrates a flow diagram of a process to prepare a composition 1, which is defined in the below description.

[0019] Fig. 2 illustrates a flow diagram of a process to prepare a composition 2, which is defined in the below description.

[0020] Fig. 3 is a graphical representation that shows growth colonies in normal mask without the application of present composition.

[0021] Fig. 4 is a graphical representation that shows growth colonies after applying present composition-1 on mask.

[0022] Fig. 5 is an image of Transmission electron microscopy (TEM) that shows the image of nanocopper particles (CuO-NPs).

[0023] Fig. 6 is a graphical representation of particle size distribution of CuO-NPs.

DETAILED DESCRIPTION OF THE INVENTION

[0024] The detailed description of various exemplary embodiments of the disclosure is described herein. It should be noted that the embodiments are described herein in such details as to communicate the disclosure. However, the amount of details provided herein is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure.

[0025] It is also to be understood that various substitutions/arrangements/permutations or combinations may be devised that, although not explicitly described or shown herein, embody the principles of the present disclosure. Moreover, all statements herein reciting principles, aspects, and embodiments of the present disclosure, as well as specific examples, intended to encompass equivalents thereof.

[0026] The terminology used herein is to describe particular embodiments only and is not intended to be limiting of example embodiments. As used herein, the singular forms “a”, “an” and “the” are intended to include the plural forms as well, unless the context indicates otherwise. It will be further understood that the terms “comprise”, “comprising”, “includes” and/or “including” when used herein, specify the presence of stated features, steps, operations, elements and/or components, but do not preclude the presence or addition of one or more other features, steps, operations, elements, components and/or groups thereof.

[0027] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which example embodiments belong. It will be further understood that terms, e.g., those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

[0028] The phrase “nanoparticle composition” can be interchangeably used with phrase “composition” in the context of the specification. The meaning of the said phrases shall be considered similar in context of the specification. The composition kills viruses, bacteria, and fungus that are present in air and surfaces. The said composition also helps in controlling infectious diseases. In particular, the said composition kills coronavirus or norovirus. The said composition contains water in large proportion i.e., in an amount of 88-98% vol. of the total composition.

[0029] The meaning of phrase “substantially alcohol free”can be interchangeably used with the phrase “alcohol free.” The meaning of the said phrase is that the said composition may contain minimal amount of alcohol. In particular, the disinfectant composition may contain alcohol in an amount of having range between 0-12 percent by vol. based on the total volume of the composition. The 0 vol. % in the range indicates that the composition does not contain any alcohol. The composition may or may not contain alcohol.

[0030] The term “copper nanoparticles” and “nanocopper” are used interchangeably in the present application. Further, “CuNPs” or “CUO-Nps” are used as abbreviated form of copper nanoparticles.

[0031] The term “pH modifiers” of the present specification means the compounds that helps in maintaining the pH of the composition. The composition include one or more pH modifiers that are selected from the group consisting of sodium carbonate, calcium chloride, ammonium chloride, sodium citrate, or a combination thereof.

[0032] The phrase “a combination thereof” in the context of the present specification means that one or more compounds can be included in the composition. For example, pH modifiers that are selected from the group consisting of sodium carbonate, calcium chloride, ammonium chloride, sodium citrate, or a combination thereof means that the composition can incorporate either one of the pH modifier or the mixture of more than one pH modifier. The examples of the pH modifier is not limited with the disclosure of the specification. The person skilled in the art may incorporate any known pH modifier in a composition of the present application that maintains the pH of the composition within the range of 6-8.

[0033] The term “surfactant” in the context of the present specification means a foaming agent that facilitates the formation of foam. The surfactant in the present application is coco-glucoside. The examples of the surfactant is not limited with the disclosure of the specification. The person skilled in the art may incorporate any known surfactant in a composition of the present application.

[0034] The term “humectant” in the context of the specification means a substance used to reduce the loss of moisture. The humectant in the present application is glycerine. The examples of the humectant is not limited with the disclosure of the specification. The person skilled in the art may incorporate any known humectant in a composition of the present application.

[0035] The essential oils used in the present disclosure are brought from Ultra International Limited.

[0036] The present disclosure relates to a nanoparticle composition. In particular, the nanoparticle composition comprising, by volume: deionized water in an amount having a range of 88%-98% of the total composition; metal nanoparticles in an amount having a range of 0.4%-0.6 % of the total composition; one or more pH modifiers having an amount in the range of 0.2% - 0.6% of the total composition; one or more surfactant having an amount in the range of 0.1%-0.3% of the total composition; one or more humectant having an amount in the range of 0.2%- 0.4% of the total composition; and one or more essential oils having an amount in the range of 0.1% - 0.2% of the total composition.

[0037] In an embodiment of the present disclosure, the nanoparticles are copper nanoparticles.

[0038] In an embodiment of the present disclosure, the copper nanoparticle are in the particle size having the range of 50 nm-100nm. In particular, the particle size of the copper nanoparticles is within in the range of 75nm-100nm. The particle size of the nanoparticles is selected from the group of 50nm, 55nm, 60 nm, 65nm, 70nm, 75nm, 80nm, 85nm, 90nm, 95nm, or 100nm. The specification also includes the copper nanoparticles having particle size falling within the above-mentioned groups, for example, particle size falling between 50nm-55nm are included. The extreme ends of the range are also included within the scope of present application.

[0039] In an embodiment of the present disclosure, the nanoparticle composition comprising deionized water, copper nanoparticles, sodium carbonate, calcium chloride, ammonium chloride, and sodium citrate.

[0040] In another embodiment of the present disclosure, the nanoparticle- composition comprising in volume (vol.)%: deionized water in an amount of 88%-98% of the total composition; copper nanoparticles in an amount of 0.4%-0.6 % of the total composition; sodium carbonate in an amount of 0.4%-0.6 % of the total composition; calcium chloride in an amount of 0.4%-0.6 % of the total composition; ammonium chloride in an amount of 0.2%-0.4 % of the total composition; and sodium citrate in an amount of 0.2%-0.4 % of the total composition.

[0041] In another embodiment of the present disclosure, the nanoparticle composition further comprises coco-glucoside and glycerin.

[0042] In another embodiment of the present disclosure, the nanoparticle based disinfectant composition comprises coco-glucoside in an amount of 0.1 vol.% - 0.3 vol.% of the total composition; and glycerin in an amount of 0.2 vol. %- 0.4 vol. % of the total composition.

[0043] In an embodiment of the present disclosure, the nanoparticle composition comprising essential oils. In particular, the nanoparticle based disinfectant composition comprises essential oils in an amount of 0.1 vol.% - 0.2 vol. % of the total composition.

[0044] In yet another embodiment of the present disclosure, wherein the essential oils are selected from the group of lemon grass oil, peppermint oil, tea tree oil, or a combination thereof. In particular, the said composition may comprise at least one essential oil or may comprise one or more of essential oils.

[0045] In yet another embodiment of the present disclosure, the nanoparticle composition may comprise alcohol.

[0046] In another embodiment of the present disclosure, the nanoparticle composition may be present in an amount of 0-12 vol. % of the total composition. The 0 vol. % of alcohol means absence of alcohol in the composition. The alcohol content in the composition is kept at minimum and has been replaced by the large proportion of water.

[0047] In another embodiment of the present disclosure, wherein the alcohol is selected from the group of methanol, ethanol, propanol, or butanol. The alcohol is not limited with the disclosure of the specification. The person skilled in the art may incorporate any known alcohol in the said composition of the present application.

[0048] In an alternative embodiment of the present disclosure, a nanoparticle composition, by vol. consisting essentially of:- deionized water in an amount of 97% based on the amount of the total composition, Copper chloride dihydrate in an amount of 0.5% based on the amount of the total composition, sodium hydroxide in an amount of 0.4% based on the amount of the total composition, sodium carbonate in an amount 0.4% based on the amount of the total composition, calcium chloride in an amount 0.4% based on the total composition, glycerin in an amount of 0.3% based on the amount of the total composition, ammonium chloride in amount of 0.2% based on the amount of the total composition, sodium Citrate in an amount of 0.2% based on the amount of the total composition, coco-glucoside in an amount of 0.2% based on the amount of the total composition; tea tree Oil in an amount of 0.2% based on the amount of the total composition, Lemongrass Oil in an amount of 0.1% based on the amount of the total composition, and Peppermint oil in an amount of 0.1% based on the amount of the total composition.

[0049] In an alternative embodiment of the present disclosure, a nanoparticle composition, by vol. consisting essentially of:- deionized water in an amount of 89% based on the amount of the total composition, propanol in an amount of 8% based on the amount of the total composition; Copper chloride dihydrate in an amount of 0.5% based on the amount of the total composition; Sodium hydroxide in an amount of 0.4%; Sodium Carbonate in an amount of 0.4% based on the amount of the total composition; Calcium Chloride in an amount of 0.4% based on the amount of total composition; Glycerin in an amount of 0.3% based on the amount of the total composition; Ammonium chloride in an amount of 0.2% based on the amount of the total composition; Sodium Citrate in an amount of 0.2% based on the amount of the total composition; Coco-glucoside in an amount of 0.2% based on the amount of the total composition; Tea tree Oil in an amount of 0.2% based on the amount of the total composition; Lemongrass Oil in an amount 0.1% based on the amount of the total composition; and Peppermint oil in an amount of 0.1% based on the amount of the total composition.

[0050] In an alternative embodiment of the present disclosure, a nanoparticle composition, by vol. consisting essentially of :- water in an amount of 97.3% based on the amount of the total composition; copper chloride dihydrate in an amount of 0.5% based on the amount of the total composition; Sodium hydroxide in an amount of 0.4% based on the amount of the total composition; Sodium Carbonate in an amount of 0.4% based on the total composition; Calcium Chloride in an amount of 0.4% based on the total composition; Ammonium chloride in an amount of 0.2% based on the amount of the total composition; Sodium Citrate in an amount of 0.2% based on the amount of the total composition; Coco-glucoside in an amount of 0.2% based on the amount of the total composition; Tea tree Oil in an amount of 0.2% based on the amount of the total composition; Lemongrass Oil in an amount of 0.1% based on the amount of the total composition; and Peppermint oil in an amount of 0.1% based on the amount of the total composition.

[0051] The nanoparticle composition of the present application is used against a microorganism selected from the group consisting of a bacterium, a fungus, a mold, and a virus. The nanoparticle composition kills coronavirus (COVID-19) or norovirus.

[0052] The nanoparticle composition of the present application is in the form of gel, solution, lotion, foam or cream. In particular, when the composition of the present application is used as the hand sanitizer composition, then the pH of the composition should be in the range of 6 to 8.

[0053] In an embodiment of the present disclosure, the composition has utility in a wide range of antimicrobial and antiviral applications. Some of these products are for personal care and coatings.

[0054] In an embodiment of the present disclosure, the nanoparticle composition is used in the composition that are selected from the group consisting of paints, fruits & vegetable wash, surface sanitizer, fabric spray, moisturizer, sunscreen, liquid soap, floor cleaner, textile conditioner, room freshener, multipurpose sanitizer, air conditioner sanitizer, heating ventilation air conditioning (HVAV) sanitizer, packaging sanitizer, disinfectant, hand sanitizer, surface cleaner, Nano coatings, or a coating formulation.

[0055] In an embodiment of the present disclosure, the composition products may be in the form of liquids, gels, solids or may be used as coatings on variety of solid objects.

[0056] In yet another embodiment of the present disclosure, the solution of copper chloride dihydrate and sodium hydroxide forms copper nanoparticle.

[0057] The present disclosure also relates to a method of preparing a method of preparing a nanoparticle composition, comprising the steps of: mixing copper chloride dihydrate and sodium hydroxide in water for a time period of 55 mins-65 mins to form solution A; mixing one or more pH modifiers in deionized water to form solution B; and heating solution B for a time period of 50 mins-70 mins till the temperature reaches 55°C-65°C; mixing one or more surfactants and humectants in deionized water for a time period of 10 hours -12 hours to form solution C; mixing essential oils in the deionized water to form solution D for a time period of 25 mins-35 mins; and mixing the solutions A-D together to form the nanoparticle composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm.

[0058] In another embodiment of the present disclosure, the components of the nanoparticle composition comprising, by volume: deionized water in an amount having a range of 88%-98% of the total composition; metal nanoparticles in an amount having a range of 0.4%-0.6 % of the total composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm; one or more pH modifiers having an amount in the range of 0.2% - 0.6% of the total composition; one or more surfactant having an amount in the range of 0.1%-0.3% of the total composition; one or more humectant having an amount in the range of 0.2%- 0.4% of the total composition; and one or more essential oils having an amount in the range of 0.1% - 0.2% of the total composition.

[0059] In another embodiment of the present disclosure, the one or more pH modifiers are selected from the group consisting of sodium carbonate, calcium chloride, ammonium chloride, sodium citrate, or a combination thereof.

[0060] In another embodiment of the present disclosure, the one or more surfactant is coco-glucoside and wherein one or more humectant is glycerin.

[0061] In another embodiment of the present disclosure, wherein the one or more essential oils are selected from the group of lemon grass oil, peppermint oil, tea tree oil, or a combination thereof.

[0062] In an embodiment of the present disclosure, the nanoparticle composition, comprising the steps of: mixing copper chloride dihydrate and sodium hydroxide in water for a time period of 55 mins-65 mins to form solution A, mixing sodium carbonate, calcium chloride, and ammonium chloride in deionized water to form solution B; and heating solution B for a time period of 50 mins-70 mins till the temperature reaches 55°C-65°C; mixing sodium citrate in deionized water to form solution C for a time period of 10 hours -12 hours; mixing essential oils in the deionized water to form solution D for a time period of 25 mins-35 mins; and mixing the solutions A-D to form the nanoparticle composition.

[0063] In yet another embodiment of the present disclosure, mixing in step f) is carried out for a time period of 22 hours - 25 hours to form the Nanoparticle based disinfectant composition.

[0064] In another embodiment of the present disclosure, the amount of the components used while preparing nanoparticle based disinfectant composition are as: deionized water is in the range of 88 vol.% - 98 vol.% of the total composition; copper nanoparticles in an amount of 0.4 vol. %-0.6 vol. % of the total composition; sodium carbonate in an amount of 0.4 vol. %-0.6 vol.% of the total composition; calcium chloride in an amount of 0.4 vol.%- 0.6 vol. % of the total composition; ammonium chloride in an amount of 0.2 vol. %-0.4 vol.% of the total composition; and sodium citrate in an amount of 0.2 vol. % - 0.4 vol. % of the total composition.

[0065] In another embodiment of the present disclosure, wherein mixing of sodium citrate in deionized water to form the solution C further comprising the step of adding glycerin and coco glucoside. The amount of coco-glucoside used in an amount of 0.1 vol. %-0.3 vol. % of the total composition; and glycerin is used in an amount of 0.2 vol. %- 0.4 vol. % of the total composition.

[0066] In an embodiment of the present disclosure, the essential oils used is in an amount of 0.1 - 0.2 vol. % of the total composition.

[0067] In another embodiment of the present disclosure, wherein the Nano-particle based disinfectant composition further comprises propanol in the amount of 0-12 vol. %.

[0068] In another embodiment of the present disclosure, wherein the solution A is solution of copper nanoparticles that forms from a chemical precipitation of the copper chloride dihydrate and the sodium hydroxide.

[0069] In another embodiment of the present disclosure, wherein the solution B is a solution of sodium carbonate, calcium chloride, and ammonium chloride in deionized water.

[0070] In another embodiment of the present disclosure, wherein the solution C is solution of sodium citrate, glycerin, and coco glucoside in deionized water.

[0071] In another embodiment of the present disclosure, wherein the solution D is solution of essential oils in the deionized water.

[0072] Advantages of the Components in the present Nanoparticle based disinfectant composition:

Nano Copper or Copper Nanoparticles (CuNPs): It was found in the present application that CuNPs extraordinary effects as antimicrobial and antibacterial. CuNPs increases the bioavailability and blood brain barrier permeability. CuNPs are obtained from copper chloride dihydrate as a source. It’s an ecofriendly disinfectant. The resultant CuNPs solution is both safe and nontoxic to people and surrounding. CuNPs provide high efficacy in killing of microorganisms in all possible media applications of air, water surface, and soil. It proves to be powerful antibacterial, fungicide, virucide that kills away wide range of microorganisms. In particular, it was found effective in killing Coronavirus (Covid-19).

Tea Tree Oil: The tea tree oil is one of the essential oil that is be effective against killing micro-organism.

Lemon Grass Oil: The lemongrass oil is found to be effective against Acinetobacter baumanii (A. baumanii), Aeromonas veronii (A. veronii), Enterococcus faecalis (E. faecalis), Escherichia coli (E. coli), Klebsiella pneumonia (K. pneumonia), Salmonella enterica (S. enterica) serotype typhimurium, Serratia marcesens (S. marcesens), Proteus vulgaris (P. vulgaris), Enterobacter aerogenes (E. aerogenes), Corynebacterium equii (C. equii) and Staphylococcus aureus (S. aureus).

Peppermint Oil: Peppermint oil possesses antibacterial activity in vitro and has moderate antibacterial effects against both Gram-positive and Gram-negative bacteria. Peppermint is also found to possess antiviral and fungicidal activities.

[0073] The invention is further illustrated by the following example, which is provided to be exemplary of the invention and does not limit the scope of the invention. While the present invention has been described in terms of its specific embodiments, certain modifications and equivalents will be apparent to those skilled in the art and are intended to be included within the scope of the present invention.

EXAMPLES

Method of preparing a water-based composition 1:
Copper chloride dihydrate and Sodium hydroxide was mixed in water for approximately 1 hour to prepare copper nanoparticles, named as Solution A. Further, sodium carbonate, calcium chloride, and ammonium chloride is mixed in deionized water to prepare a solution B and heating heat it till 60OC for approximately 1 hour. Sodium citrate, glycerin, and coco glucoside are mixed in deionized water to form solution C for 12 hours. Further, tea tree oil, lemon grass oil, and peppermint oil are mixed in deionized water to form solution D for 30 mins. Then, final solution was prepared by mixing all the solutions (A, B, C, & D) for 24 hours to prepare a disinfectant composition.

Table 1: Concentration of the components in composition 1

Component Name % Composition Range (v/v)
Regent Grade Triple Deionized Water 97
Copper Chloride dihydrate 0.5
Sodium hydroxide 0.4
Sodium Carbonate 0.4
Calcium Chloride 0.4
Glycerin 0.3
Ammonium Chloride 0.2
Sodium Citrate 0.2
Coco-glucoside 0.2
Melaleuca Alternifolia (Tea Tree oil) 0.2
Combopogan Citraus (Lemongrass oil) 0.1
Peppermint oil 0.1

Method of preparing a water-based composition 2:
Copper chloride dihydrate, propanol, and Sodium hydroxide were mixed in water for approximately 1 hour to form copper nanoparticles, named as Solution A. Further, sodium carbonate, calcium chloride, and ammonium chloride were mixed in deionized water to form solution B and heating it till 60OC for approximately 1 hour. Sodium citrate, glycerin, and coco glucoside in deionized water were mixed to form solution C for 12 hours. Further, tea tree oil, lemon grass oil, and peppermint oil were mixed in deionized water to form solution D for 30 mins. Then, final solution was prepared by mixing all the solutions (A, B, C, & D) for 24 hours to prepare a disinfectant composition.

Table 2: Concentration of the components in composition 2
Component Name % Composition Range (v/v)
Regent Grade Triple Deionized Water 89
Propanol 8
Copper Chloride dihydrate 0.5
Sodium hydroxide 0.4
Sodium Carbonate 0.4
Calcium Chloride 0.4
Glycerin 0.3
Ammonium Chloride 0.2
Sodium Citrate 0.2
Coco-glucoside 0.2
Melaleuca Alternifolia (Tea Tree oil) 0.2
Combopogan Citraus (Lemongrass oil) 0.1
Peppermint oil 0.1

Antimicrobial activity of the composition 1:-

Table 3: Data for Antimicrobial activity of composition 1
Name of microorganism Spinking Range After 1 min After 15 mins After 30 mins
Cfu/ml Log Value Cfu/ml % reduction Cfu/ml % reduction Cfu/ml % reduction
Escherichia Coli 2.4 X 107 7.38 305 99.99 62 99.99 13 99.99
Staphylococcus Aureus 3.2 X 107 7.50 623 99.99 77 99.99 9 99.99

The analysis of the antimicrobial activity was carried out for composition 1, under dirty conditions (bovine serum albumin 3 g/L and erythrocytes 3mL/L), at 100% concentration (pure), and during 30 seconds of exposure. The composition 1 shows virucidal activity against the mandatory viruses, Adenovirus type 5 and Murine Norovirus. The activity is evaluated according to NF EN 16777:2018 guideline. Analysis is based on the method ASTM E2315 (Assessment of Antimicrobial Activity Using a Time-Kill Procedure).

The composition 1, batch not indicated, under dirty conditions, pure (100%) and diluted at 50%, and during 30 seconds of exposure, shows virucidal activity against Coronavirus 229E, with a mean reduction of 5.29 ± 0.40 TCID50, for both concentrations and two replicates, compared with the virus control after 30 seconds of exposure (T* - mean = 5.79 ± 0.40 TCID50) when the activity is assayed according with the NF EN 16777: 2018 guideline.

Table 4: Effect of composition 1 on spraying it on normal mask
S. No. FORMULATIONS LOG 5 (E-COLI BACTERIA) LOG 5 (E-COLI BACTERIA) LOG 5 (E-COLI BACTERIA)
1. REFERENCE 53 42 21
2. 60% PROPANOL 135 112 23
3. COMPOSITION -1 13 6 0
The composition-1 was sprayed on the normal mask, no growth of bacteria colony occurred for around 6 hours. Please refer to figure no. 4 which shows that no growth colony was observed in comparison with the normal ask. Further, in case of spraying only propanol on normal mask the bacteria colonies increased exponentially. Furthermore, the anti-allergic and irritation test was conducted as per the guidelines of Drugs and Cosmetics Act, 1940 and the said tests came out negative.

Antimicrobial activity of the composition 2:-

Table 5: Data for Antimicrobial activity of composition 2
Name of microorganism Spinking Range After 1 min After 30 mins After 60 mins
Cfu/ml Log Value Cfu/ml % reduction Cfu/ml % reduction Cfu/ml % reduction
Escherichia Coli 3.4 X 107 7.53 823 99.99 65 99.99 14 99.99
Staphylococcus Aureus 2.6 X 107 7.41 132 99.99 48 99.99 12 99.99

The analysis of the antimicrobial activity was carried out for composition 2, under dirty conditions (bovine serum albumin 3 g/L and erythrocytes 3mL/L), at 100% concentration (pure), and during 30 seconds of exposure.
Transmission electron microscope
Transmission electron microscopy (TEM) has been carried out to find out the size of CuO-NPs (Fig. 5) formed by the process of the present application. The TEM research was carried out on a 200 kV operating HITACHI H-800. Most of the CuO-NPs were sphere-shaped and suspended well in the solution within the size range of 50–100 nm and are shown in (Fig. 6). The standard particle size was around 75 nm. 90% of particles were under 100 nm of size.

SPECIFIC EMBODIMENTS OF THE INVENTION

[0074] The present disclosure relates to a nanoparticle composition comprising, by volume: deionized water in an amount having a range of 88%-98% of the total composition; metal nanoparticles in an amount having a range of 0.4%-0.6 % of the total composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm; one or more pH modifiers having an amount in the range of 0.2% - 0.6% of the total composition; one or more surfactant having an amount in the range of 0.1%-0.3% of the total composition; one or more humectant having an amount in the range of 0.2%- 0.4% of the total composition; and one or more essential oils having an amount in the range of 0.1% - 0.2% of the total composition.

[0075] Such Nanoparticle composition is disclosed, wherein the one or more pH modifiers are selected from the group consisting of sodium carbonate, calcium chloride, ammonium chloride, sodium citrate, or a combination thereof.

[0076] Such Nanoparticle composition, wherein the surfactant is coco-glucoside and wherein the humectant is glycerin.

[0077] Such Nanoparticle composition, wherein the surfactant is coco-glucoside and wherein the humectant is glycerin.

[0078] Such Nanoparticle disinfectant composition is disclosed, wherein the essential oils are selected from the group of lemon grass oil, peppermint oil, tea tree oil, or a combination thereof.

[0079] Such Nanoparticle composition is disclosed that further comprises an alcohol in an amount of 0-12 vol. % of the total composition, wherein the alcohol is propanol.

[0080] Such Nanoparticle composition is disclosed, wherein the nanoparticle composition is used against a microorganism selected from the group consisting of a bacterium, a fungus, a mold, and a virus.

[0081] Such Nanoparticle composition is disclosed, wherein the virus is coronavirus (COVID-19) or norovirus.

[0082] Such Nanoparticle composition is disclosed, wherein the nanoparticle composition is used in the composition that are selected from the group consisting of paints, disinfectant, hand sanitizer, surface cleaner, or a coating formulation.

[0083] Such Nanoparticle composition is disclosed, wherein the nanoparticle composition is in the form of gel, solution, lotion, foam or cream.

[0084] Such Nanoparticle composition is disclosed, wherein the hand sanitizer composition has pH in the range of 6 to 8.

[0085] The present disclosure also relates to a method of preparing a nanoparticle composition, comprising the steps of:
a) mixing copper chloride dihydrate and sodium hydroxide in water for a time period of 55 mins-65 mins to form solution A;
b) mixing one or more pH modifiers in deionized water to form solution B; and
c) heating solution B for a time period of 50 mins-70 mins till the temperature reaches 55°C-65°C;
d) mixing one or more surfactants and humectants in deionized water for a time period of 10 hours -12 hours to form solution C;
e) mixing essential oils in the deionized water to form solution D for a time period of 25 mins-35 mins; and
f) mixing the solutions A-D together to form the nanoparticle composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm.
[0086] Such method is disclosed wherein the components of the nanoparticle composition comprising, by volume:
deionized water in an amount having a range of 88%-98% of the total composition;
metal nanoparticles in an amount having a range of 0.4%-0.6 % of the total composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm;
one or more pH modifiers having an amount in the range of 0.2% - 0.6% of the total composition;
one or more surfactant having an amount in the range of 0.1%-0.3% of the total composition;
one or more humectant having an amount in the range of 0.2%- 0.4% of the total composition; and
one or more essential oils having an amount in the range of 0.1% - 0.2% of the total composition.

[0087] Such method is disclosed, wherein the one or more pH modifiers are selected from the group consisting of sodium carbonate, calcium chloride, ammonium chloride, sodium citrate, or a combination thereof.

[0088] Such method is disclosed, wherein the surfactant is coco-glucoside and wherein the humectant is glycerin.

[0089] Such method is disclosed, the one or more essential oils are selected from the group of lemon grass oil, peppermint oil, tea tree oil, or a combination thereof.

[0090] Such method is disclosed, wherein step c further comprising the step of adding glycerin and coco glucoside.

[0091] Such method is disclosed, wherein mixing in step f) is carried out for a time period of 22 hours-25 hours to form the Nano-particle disinfectant composition.

[0092] Such method is disclosed, wherein the Nanoparticle disinfectant composition further comprises propanol in the amount of 0-12 vol.%

[0093] Such method is disclosed, wherein the solution A is a solution of copper nanoparticles that forms from a chemical precipitation of the copper chloride dihydrate and the sodium hydroxide.

INDUSTRIAL APPLICABILITY

The nanoparticle composition of the present disclosure kills coronavirus (COVID-19) or norovirus. It has anti-fungal and anti-microbial activities. The nanoparticle composition can be used in the composition that are selected from the group consisting of paints, disinfectant, hand sanitizer, surface cleaner, or a coating formulation. It is environmentally benign and easily available. It is a herbal composition. which is safe and abundantly available and is cost efficient.

CLAIMS:We Claim:

1. A nanoparticle composition comprising, by volume:
deionized water in an amount having a range of 88%-98% of the total composition;
metal nanoparticles in an amount having a range of 0.4%-0.6 % of the total composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm;
one or more pH modifiers having an amount in the range of 0.2% - 0.6% of the total composition;
one or more surfactant having an amount in the range of 0.1%-0.3% of the total composition;
one or more humectant having an amount in the range of 0.2%- 0.4% of the total composition; and
one or more essential oils having an amount in the range of 0.1% - 0.2% of the total composition.

2. The nanoparticle composition as claimed in claim 1, wherein the one or more pH modifiers are selected from the group consisting of sodium carbonate, calcium chloride, ammonium chloride, sodium citrate, or a combination thereof.

3. The nanoparticle composition as claimed in claim 1, wherein the surfactant is coco-glucoside and wherein the humectant is glycerin.

4. The nanoparticle composition as claimed in claim 1, wherein the one or more essential oils are selected from the group of lemon grass oil, peppermint oil, tea tree oil, or a combination thereof.

5. The nanoparticle composition as claimed in claim 1, further comprising an alcohol in an amount of 0-12 vol. % of the total composition, wherein the alcohol is propanol.

6. The nanoparticle composition as claimed in claim 1, wherein the nanoparticle composition is used against a microorganism selected from the group consisting of a bacterium, a fungus, a mold, and a virus.

7. The nanoparticle composition as claimed in claim 6, wherein the virus is coronavirus (COVID-19) or norovirus.

8. The nanoparticle composition as claimed in claim 1, wherein the nanoparticle composition is used in the composition that are selected from the group consisting of paints, disinfectant, hand sanitizer, surface cleaner, or a coating formulation.

9. The nanoparticle composition as claimed in claim 1, wherein the nanoparticle composition is in the form of gel, solution, lotion, foam or cream.

10. The nanoparticle composition as claimed in claim 8, wherein the hand sanitizer composition has pH in the range of 6 to 8.

11. A method of preparing a nanoparticle composition, comprising the steps of:
a) mixing copper chloride dihydrate and sodium hydroxide in water for a time period of 55 mins-65 mins to form solution A;
b) mixing one or more pH modifiers in deionized water to form solution B; and
c) heating solution B for a time period of 50 mins-70 mins till the temperature reaches 55°C-65°C;
d) mixing one or more surfactants and humectants in deionized water for a time period of 10 hours -12 hours to form solution C;
e) mixing essential oils in the deionized water to form solution D for a time period of 25 mins-35 mins; and
f) mixing the solutions A-D together to form the nanoparticle composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm.

12. The method as claimed in claim 11, wherein the components of the nanoparticle composition comprising, by volume:
deionized water in an amount having a range of 88%-98% of the total composition;
metal nanoparticles in an amount having a range of 0.4%-0.6 % of the total composition, wherein the metal nanoparticles are copper nanoparticles, and wherein the particle size of the nanoparticles is within the range of 50 nm-100 nm;
one or more pH modifiers having an amount in the range of 0.2% - 0.6% of the total composition;
one or more surfactant having an amount in the range of 0.1%-0.3% of the total composition;
one or more humectant having an amount in the range of 0.2%- 0.4% of the total composition; and
one or more essential oils having an amount in the range of 0.1% - 0.2% of the total composition.

13. The method as claimed in claim 12, wherein the one or more pH modifiers are selected from the group consisting of sodium carbonate, calcium chloride, ammonium chloride, sodium citrate, or a combination thereof.

14. The method as claimed in claim 11, wherein the surfactant is coco-glucoside and wherein the humectant is glycerin.

15. The method as claimed in claim 11, wherein the one or more essential oils are selected from the group of lemon grass oil, peppermint oil, tea tree oil, or a combination thereof.

16. The method as claimed in claim 11, wherein mixing in step f) is carried out for a time period of 22 hours-25 hours to form the nanoparticle composition.

17. The method as claimed in claim 11, wherein the nanoparticle composition further comprises propanol in the amount of 0-12 vol.%

18. The method as claimed in claim 11, wherein the solution A is a solution of copper nanoparticles that forms from a chemical precipitation of the copper chloride dihydrate and the sodium hydroxide.