FORM 2
THE PATENTS ACT, 1970
(39 of 1970)
&
The Patents Rules, 2005
COMPLETE SPECIFICATION
(See section 10 and rule 13)
TITLE OF THE INVENTION
A neem oil and turpentine oil based wound healing ointment or cream
APPLICANTS

Name : Godrej Agrovet Limited
Nationality : an Indian Company
Address : Pirojshanagar, Eastern Express
Mumbai 400079, Maharashtra, India

Highway, Vikhroli (East),

PREAMBLE TO THE DESCRIPTION
The following specification particularly describes the nature of this invention and the
manner in which it is to be performed


FIELD OF INVENTION
This invention relates to a neem oil and turpentine oil based wound
healing ointment or cream.
PRIOR ART
Antibacterial and antifungal properties of neem oil and turpentine oil
are known. It is, however, difficult and cumbersome to apply and
retain neem oil and turpentine oil on wounds for healing purpose due to
the free flowing nature of the oils. Therefore, use of neem oil and
turpentine oil for wound healing application is inconvenient,
cumbersome and time consuming. Turpentine oil is also known to act as
maggot repellent. Regeneration of tissues is an integral part of healing.
Activities such as cell proliferation, smoothening of the skin or restoration
of the epithelial cells also facilitate healing. Linseed oil, zinc oxide and
salicylic acid are reported to possess these activities. Antiseptic properties of
salicylic acid and zinc oxide are also reported. [The Merck Index - 12th Edn
1996, Publ: Merck & Co. Inc. NJ USA]
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OBJECTS OF INVENTION
An object of the invention is to provide a neem oil and turpentine oil
based wound healing ointment or cream whicn has improved wound
healing properties.
Another object of the invention is to provide a neem oil and turpentine
oil based wound healing ointment or cream which is easy and
convenient to apply on wounds.
Another object of the invention is to provide a neem oil and turpentine
oil based wound healing ointment or cream which is economical.
Another object of the invention is to provide a neem oil and turpentine
oil based wound healing ointment or cream which is without side
effects and is safe.
Another object of the invention is to provide a process of preparing a
neem oil and turpentine oil based wound healing ointment or cream
which has improved wound healing properties.
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Another object of the invention is to provide a process of preparing a
neem oil and turpentine oil based wound healing ointment or cream
which is easy and convenient to apply on wounds.
Another object of the invention is to provide a process of preparing a
neem oil and turpentine oil based wound healing ointment or cream
which is economical.
Another object of the invention is to provide a process of preparing a
neem oil and turpentine oil based wound healing ointment or cream which
is without side effects and is safe.
Another object of the invention is to provide a process of preparing a
neem oil and turpentine oil based wound healing ointment or cream which
is simple, easy and convenient to carry out.
Another object of the invention is to provide a method of treatment of
wounds by applying topically the above neem oil and turpentine oil based
wound healing ointment or cream.
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DESCRIPTION OF THE INVENTION
According to the invention there is provided a neem oil and turpentine oil
based wound healing ointment or cream comprising 10 to 20% by weight of
neem oil and turpentine oil in the volume ratio 1:4 to 4:1, 1.5 to 3.0 % by
weight of zinc oxide, 0.5 to 2.0% by weight of salicylic acid, 0.5 to 2.0%
by weight of potassium nitrate, 5 to 15% by weight of linseed oil and 50 to
80% by weight of additive base.
Preferably the additive base comprises bees wax and petroleum
jelly in the weight ratio 15 : 40 to 25 : 70.
Preferably the wound healing ointment or cream comprises 16.5% by
weight of neem oil and turpentine oil in the volume ratio 3:1, 1.65% by
weight of zinc oxide, 0.8% by weight of salicylic acid, 0.8% by weight
of potassium nitrate, 10.0% by weight of linseed oil and 70.25% by
weight of additive base, namely bees wax and petroleum jelly in the
weight ratio 23 : 77.
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According to the invention there is also provided a process of
preparing a neem oil and turpentine oil based wound healing ointment or
cream comprising the steps of
(i) melting 50 to 80% by weight of an additive base at 40 to
100°C;
(ii) mixing 10 to 20% by weight of neem oil and turpentine oil in the
volume ratio 1:4 to 4:1 and 5 to 15% by weight of linseed oil with the
additive base melt under stirring followed by cooling the mixture to
20 to 50 ° C;
(iii) and further mixing the mixture obtained by step (ii) with 1.5 to
3.0% by weight of zinc oxide, 0,5 to 2.0% by weight of salicylic acid
and 0.5 to 2.0% by weight of potassium nitrate under stirring.
Preferably process comprises melting the additive base namely bees
wax and petroleum jelly in the weight ratio 15 : 40 to 25 : 70.
Preferably the process comprises the steps of
ia) melting the additive base, namely bees wax and petroleum jelly in
the weight ratio 23 : 77;
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iia) mixing 16.5% by weight of neem oil and turpentine oil in the
volume ratio 3:1 and 10.0% by weight of linseed oil with the additive
base melt followed by cooling;
iiia) and further mixing the mixture obtained by step (iia) with 1.65%
by weight of zinc oxide, 0.8% by weight of salicylic acid and 0.8% by
weight of potassium nitrate.
Preferably the additive base is melted at 75 ° C.
Preferably the mixture of step (ii) or (iia) is cooled to 40 ° C.
Besides bees wax and petroleum jelly other additive base such as paraffin
wax, salts and esters of fatty acids, fatty alcohols or hydrogenated wax may
also be used in the ointment or cream of the invention.
According to the invention there is also provided a method of
treatment of wounds by applying topically the neem oil and turpentine
oil based wound healing ointment or cream described above.
The medicament of the invention may be used on any mammalian species.
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This application has been divided out of our patent application No
486/MUM/2004 filed on 27th April 2004.
The following experimental example is illustrative of the invention but not
limitative of the scope thereof.
Example 1
Bees wax (16.5% by weight) and petroleum jelly (53.75% by weight)
were melted at 75 °C. Neem oil and turpentine oil (16.5% by weight in the
volume ratio 3:1) and linseed oil (10.0% by weight) were mixed with the
melt under stirring for half an hour. After cooling the mixture to 40°C, zinc
oxide (1.65% by weight), salicylic acid (0.8% by weight) and potassium
nitrate ( 0.8% by weight) were further mixed with the above mixture
under stirring for one hour to form an ointment. The wound healing
efficacy of the ointment was studied on fresh wounds in dogs and
cattle and the results were as given in the following Tables:
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Table 1
Data on wound healing in dogs

Medicament Time taken to start
wound healing in days
(Mean ± S.E) Time taken for
complete wound
healing in days (Mean
±SE)
Neem oil and
turpentine oil in the
volume ratio 3:1 4.41 ±0.51 21.34 ±2.64
Ointment of Example
1 2.83 ±0.33 16.50 ±2.38
Acrilin ointment 2.33 ±0.33 15.33 ±1.05
Table 2
Data on wound healing in cattle

Medicament Time taken to start
wound healing in days
(Mean ± S.E) Time taken for
complete wound
healing in days (Mean
+SE|)
Neem oil and
turpentine oil in the
volume ratio 3:1 5.12 ± 0.48 36.2 ±2.80
Ointment of Example
1 2.67 ±0.33 29.5 ± 2.29
Acrilin ointment 3.0 ± 0.26 30.33 ±2.65
The frequency of application of the medicaments on the wounds was
twice daily namely morning and evening. Acrilin is a commercially
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available ointment of GSK Agrivet, India comprising gamma benzene
hexachloride, profiavin hemisulphate and cetrimide.
It is quite evident from Tables 1 and 2 that the ointment of the
invention showed improved wound healing properties as compared to
neem oil and turpentine oil alone and that the wound healing properties
of the ointment of the invention were comparable to those of the
commercially available ointment Acrilin. The ointment of the invention is
easy and convenient to apply on wounds. It is economical because
neem oil and turpentine oil and the other ingredients are cheap and easily
available. The ointment of the invention is also safe and without side
effects as the ingredients are without side effects. Besides being in the
form of ointment, the medicament of the invention also may be in the
form of a cream. The process of preparing the ointment or cream of
the invention is simple, easy and convenient to carry out.
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WE CLAIM :
1) A neem oil and turpentine oil based wound healing ointment or
cream comprising 10 to 20% by weight of neem oil and turpentine oil in
the volume ratio 1:4 to 4:1, 1.5 to 3.0% by weight of zinc oxide, 0.5
to 2.0% by weight of salicylic acid, 0.5 to 2.0 % by weight of potassium
nitrate, 5 to 15% by weight of linseed oil and 50 to 80% by weight of
additive base.
2) A neem oil and turpentine oil based wound healing ointment
or cream as claimed in claim 1, wherein the additive base comprises
bees wax and petroleum jelly in the weight ratio 15 : 40 to 25 : 70.
3) A neem oil and turpentine oil based wound healing ointment or
cream as claimed in claim 1, comprising 16.5% by weight of neem oil
and turpentine oil in the volume ratio 3:1, 1.65% by weight of zinc oxide,
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0.8% by weight of salicylic acid,0.8% by weight of potassium nitrate,
10.0% by weight of linseed oil and 70.25% by weight of additive base
namely bees wax and petroleum jelly in the weight ratio 23 : 77.
Dated this 9th day of March 2005

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