Description:
The following specification particularly describes the invention and the manner in which it is to be performed:

TECHNICAL FIELD

[001] The present invention relates to novel composition as wax moth repellent for the apiculture store room.

BACKGROUND
[002] Wax moth is the ubiquitous pest of honeybees globally. Wax moth larva could be a terrible menace to the beekeepers as it turns the entire wax combs to a pile of debris and frass. Wax moth larva is a voracious eater tends to feed on wax combs, cast off larval bee skin, pollen, brood and honey more seriously forcing entire colony to abscond.
[003] Galleria mellonella or the greater wax moth commonly called as wax moth or web worm or honeycomb moth includes different species of moth which invade, attack, damage and ultimately destroy honeybee comb. Around the globe for beekeepers and honeybee colonies the wax moth remains a vexing problem.
[004] Chandel et al. (2003) stated the cosmopolitan distribution of wax moth is attributed by its feeding habits and tunnelling through the combs. Initially, the larvae bore into the comb later, making silken threads into the middle of the comb and with the course of the time entire comb gets covered with the mass of silken webbing and faecal matter leads to a condition called “Galleriasis” (Lalita et al., 2017; Hosamani et al., 2000).
[005] Wax moth cause damage only during the larval stage as the mature insect has atrophied mouth parts. The larvae feed on honey, pollen, brood, cast off pupal skin of honeybee, wax as well as the impurities found in beeswax itself (Jindra and Sehnal, 1989). The wax moth simply takes advantage of unattended combs and lays large numbers of eggs, from which larvae emerge and eat the comb turning it into the pile of debris. Kumar et al. (2018) stated that every year wax moth causes financial losses in the apiculture industry which has raised a serious concern among the apiculturists, scientists as well as the general public.
[006] The above stated problem forced the researchers for concentrating on the management of wax moth includes conventional, physical, chemical and non-chemicals methods including use of biological agents. Tsegaye et al. (2014) suggested non-chemical methods involve the use of moth traps, tobacco leaf smoking and supplementary feeding of the honeybee colony and usage of wax moth repellent dhoop and volatile oil etc.
[007] AU2021103106A4, the present utility model relates to the field of pest control devices in Chinese honey bee apiaries, in particular to a device for trapping and killing wax moth.

[008] US20090104288A1, this invention is directed to methods and compositions to prevent or treat a honey bee pathogen infection (e.g., Melissococcus plutonis, Paenibacillus larvae, Ascosphaera apis). Moreover, the invention provides for the treatment or prevention of hive infestations with Lepidopteran pests, such as the wax moth (Galleria mellonella).

[009] RU2001132922A, this invention provided the method of combating wax moth, including treating beehives and honeycombs in storages with a biologically active substance, characterized in that ozone is used as a biologically active substance.
[0010] IN202011032625, this invention provided the method of formulation of natural spray which contains essential oils for curbing the growth of wax moth in a bee hive. The spray contains natural ingredients which do not harm the colony of honey bee and affects only the wax moth which is spoiling the honey bee.
[0011] Lovleen et al in 2021 reviewed about the wax moth i.e Galleria mellonella which is proved to be blessing or blight. (Journal of entomological research, 45).
[0012] None of the prior art discloses the formulation and development of novel composition as wax moth repellent.

SUMMARY
[0013] The present invention discloses the formulation and development of a volatile composition as wax moth repellent for apiculture store room.
[0014] In one of the object of the invention discloses the composition of the said formulation.
[0015] In another implementation, the invention discloses the methodology of this volatile formulation.

BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The foregoing detailed description of embodiments is better understood when read in conjunction with the appended drawings.

DETAILED DESCRIPTION

[0017] The disclosed embodiments are merely exemplary of the disclosure, which may be embodied in various forms
[0018] The target of present work is to develop the volatile formulation for wax moth adults. Wax moth is devastating pest of apiculture which destroy bees wax frames and honey bee comb, therefore present aim is to control adult wax moth which sit on roof of store room.
[0019] In exemplary embodiment, the firstly all the herbs are collected, weighed, dried. Further, the essential oil is separated from each herb using different extraction methods. The diethyl phthalate is used as solvent in the preparation. Later on, the formulation is assessed for effectivity on adult wax moth. Furthermore, thermal stability of product will be analyzed at different temperature range. The formulation stability will be analyzed by considering stability of ingredients.

[0020] In one embodiment, the invention composition of the volatile formulation as wax moth repellent for apiculture store room is disclosed. The volatile formulation is composite mixture of essential oils of Citronella, Datura, Zingiber officinale (ginger), Cinnamomum verum (Dalchini), Syzygium aromaticum (Clove), Trachyspermum ammi (Ajwain) and Withania somnifera (ashwagandha) dissolved in Diethyl phthalate.

[0021] Furthermore, volatile oil from the different herbs are used for the preparation of said formulation. The volatile oil from Citronella, Datura, Cinnamomum verum, Syzygium aromaticum, Trachyspermum ammi, Zingiber officinale are collected by defined methods. The diethyl phthalate is used as solvent in the preparation. The list of ingredients is given in table 1. These all ingredients are used for their antibacterial properties. All the ingredients are purchased form the local vendor in Jalandhar, Punjab.
Table 1: List of ingredients

[0022] Furthermore, table 2 indicated the quantities of the volatile oil form the specified herbs to be used in the said formulation.
Table 2: Quantities of ingredients

[0023] In one another embodiment, the methodology of the said preparation is given.
[0024] Furthermore, the essential oils of different ingredients are dissolved in various proportionalities in solvent Diethyl phthalate. This volatile formulation can be used with involvement of any mosquito repellent machine. Essential oil of Syzygium aromaticum act as thermal stabilizer, whereas Citronella help in imparting fragrance.
[0025] Furthermore, the formulation is further assessed effectivity on adult wax moth. Furthermore, thermal stability of product are analyzed at different temperature range. The formulation stability is analyzed by considering
stability of ingredients.

Claims:
1. A novel composition as wax moth repellent consisting of: Citronell; Datura; Zingiber officinale; Cinnamomum verum; Syzygium aromaticum; Trachyspermum ammi; Withania somnifera; Diethyl phthalate in the ratio of (0.5-2.5): (0.5-1.2): (0.5-1.0): (0.5-2.8): (0.5-1.5): (1.8-2.3): (0.75-1.75): (25.0-30.3).
2. A method for preparing novel composition of wax moth repellent, the said method comprises the step of:

a) Isolation of essential oil from the herbs by using suitable method;
b) Dissolution of the essential oil in the solvent;
c) Mixing of all the components at room temperature.

3. A novel composition as wax moth repellent a claimed in claim 1 wherein the essential oil of the said plants is used as active ingredient.