The present invention relates to the field of agrochemicals. The invention in particular relates to a pesticidal combination for simultaneous control of insect and fungal pests in agricultural crops.
BACKGROUND OF THE TNVENTTON:
Damage to economically important plants and crops caused by various pests including insects, weeds, fungus, bacteria, virus, rodents, nematodes and other pests greatly impair the productivity and, in some instances completely destroy the crop. At certain instances, plants also may suffer from simultaneous or subsequent attack by various kind of pests for instance an insect pest and a plant disease. Since each pest has its unique mechanism of damaging the plant crop, specific active ingredient may be required to control a target pest especially under the circumstances of concurrent attack by the different kind of pests.
Few prior arts disclose certain pesticidal compositions comprising insecticidal active ingredient and fungicidal active ingredient. For instance, WO2008135480 discloses a laundry list comprising azacona-zole, bitertanol, bromu-conazole, cypro-conazole, difenoconazole, diniconazole, diniconazole-M, enilconazole, epoxiconazole, fluquin-conazole, fenbuconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, pencon-azole, propiconazole, prothiocon-azole, simeconazole, tri-adimefon, triadimenol, tebuconazole, tetraconazole, triti-conazole, prochloraz, pe- furazoate, imazalil, triflumizole, cyazofamid, benomyl, carbendazim, thiabenda-zole, fuberidazole, ethaboxam, etridiazole, hymexazole and oxpoconazol. However, WO2008135480 fails to disclose the effective concentration and specific combination of insecticidal and fungicidal active ingredients.
EP2157857 also discloses a composition comprising strigolactone derivative, insecticidal active ingredient and fungicidal active ingredient. However, EP2157857 fails to disclose the particular combination comprising insecticidal and fungicidal active ingredients used at a specific concentration or range.
None of the prior art documents however disclose a combination comprising the insecticidal- fungicidal mix aimed at delivering the simultaneous and effective control of

both fungus and insect pests in agricultural crops. Therefore, there arises a need to develop a pesticidal combination that delivers a simultaneous and enhanced control of both the insect and fungal pests attacking the economically important crops
OBJECT OF THE INVENTION
An object of the present invention is to provide a novel and synergistic pesticidal combination that provides simultaneous control of a specific insect and a specific fungal pest in agricultural crops.
SUMMARY OF THE INVENTION
The present invention provides a novel and synergistic pesticidal combination that provides simultaneous control of insect and fungal pests in agricultural crops. The combination of the present invention may comprise insecticides in combination with pesticides which provides a multipronged approach for management of crop infestations. The present invention also discloses compositions comprising the combination of the present invention and agricultural use of the combination and composition of the present invention.
DETAILED DESCRIPTION OF THE INVENTION:
Those skilled in the art will be aware that the invention described herein is subject to variations and modifications other than those specifically described. It is to be understood that the invention described herein includes all such variations and modifications. The invention also includes all such steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations of any two or more of said steps or features.
In an embodiment, the present invention discloses a pesticidal combination comprising at least two insecticidal active ingredients and at least one fungicidal active ingredient.
The present invention discloses a synergistic pesticidal combination wherein said combination comprises:

a) At least one insecticide belonging to the group of neonicotinoids;
b) At least one insecticide belonging to the group of pyridines azomethines;
c) At least one fungicide belonging to the group of benzimidazole;
The insecticidal active ingredients of present invention may be selected from a group comprising neonicotinoids including but not limited to Acetamiprid, Clothianidin, Imidacloprid, Nitenpyram, Nithiazine, Dinotefuran, Thiacloprid and Thiamethoxam. Preferably the insecticide active ingredients are Nitenpyram.
Nitenpyram, known by its CAS NUMBER: 150824-47-8 and IUPAC name (lE)-N-((6-chloro-3-pyridinyl)methyl)-N-ethyl-N-methyl-2-nitro-l,l- ethenediamine is repreented by the chemical structure below.

Nitenpyram is a pale yellow crystalline solid in its solid form. It is a systemic insecticide that belongs to the chemical class of neonicotinoids. It acts as primarily against hemipteran but also with some activity against thysanoptera. As other neonicotinoids, Nitenpyram is an agonist of the nicotinic acetylcholine receptors it has systemic mode of action with trans- laminar activity. It possesses stomach and contact action affecting the insect nervous system.
It however does not show any long-term activity. It is largely used to kill external parasites in livestock and domestic pets but also used to control sucking insects on rice and in greenhouse crops. Example of pests controlled by this chemical include aphids, thrips, whitefly, fleas and ticks.

The insecticidal active ingredients of present invention may be selected from a group comprising at-least one insecticide belonging to the group of pyridines azomethines, preferably the compound is pymetrozin.
Pymetrozine, having the CAS NUMBER: 123312-89-0 also known as 6-methyl-4-[(E)-pyridin-3-ylmethylideneamino]-2,5-dihydro-l,2,4-triazin-3-one and represented by the figure below is a relatively new insecticide.

It is highly active and specific against sucking insect pests. Pymetrozine is the only representative of the pyridine azomethines, a comparatively new class of insecticides, and is being developed worldwide for control of aphids and whiteflies in field crops, vegetables, ornamentals, cotton, hop, deciduous fruit, and citrus, and for the control of plant hoppers in rice. Pymetrozine technical is a crystalline granular in its solid state with a melting point of 217°C (decomp.). The mode of action of pymetrozine in insects has not been precisely determined biochemically, but it may involve effects on neuro-regulation or nerve-muscle interaction. Physiologically, it appears to act by preventing these insects from inserting their stylus into plant tissue, thus inhibiting the insects to feed themselves.
The fungicidal active ingredients of the presently disclosed composition may be selected from a group comprising benzimidazoles such as Carbendazim, (MBC), thiophanate-methyl, thiabendazole, fuberidazole, Albendazole, Benomyl, Chlorfenazole, cypendazole, debacarb, mecarbinzid and Rabenzazole, more preferably carbendazim.
Carbendazim with CAS No. 10605-21-7 also represented as methyl N-(lH-benzimidazol-2-yl) carbamate is a fungicide of the benzimidazole. Its chemical structure is as below:

V

OCH-

Carbendazim is a systemic fungicide with protective and curative action. It functions by inhibiting beta-tubulin synthesis. It is absorbed through the roots and green tissues with acropetal translocation. It particularly acts by inhibiting the development of germ tubes, formation of appresoria and the growth of mycelia. The fungicide is used to control plant diseases in cereals and fruits, including citrus, bananas, strawberries, pineapples, and pomes.
The pesticidal combination of the present invention may comprise insecticidal active ingredients in the range 1-100% and fungicidal active ingredient in the range of 1-60%.
The pesticidal combination of the present invention preferably comprises
a) Nitenpyram in the range of 1-30% w/w of the total weight, preferably 1-28% w/w, more preferably 1-25% w/w of the combination,
b) Pymetrozine in the range of 1-70% w/w, preferably 1-68% w/w, more preferably 1-65% w/w of the total weight of the combination.
c) Carbendazim in the range of 1-60% w/w, preferably 1-58% w/w, more preferably 1-55% w/w of the total weight of the combination.
The synergistic pesticidal combination may be present in the ratio of 1:1:1 to 1:3:3.
In another embodiment, the present invention discloses a composition comprising the combination of the present invention along with agriculturally acceptable excipients selected from the group comprising suspension concentrates, highly concentrated aqueous suspensions, wet dispersible powders, water dispersible granules, dispersions and aqueous solutions.
The composition of the present invention may be any suitable composition such as wet dispersible granules, suspensions, emulsions, preferably suspension concentrates.

The pesticidal composition of the present invention may be formulated as suspension, highly concentrated aqueous suspensions, wet dispersible powders, water dispersible granule, dispersions or aqueous solution.
The suspension concentrate of the present invention comprises an anti-freeze agent, a dispersing agent, a defoaming agent, a thickener and an antibacterial agent.
The present composition comprises one or more anti-freeze agent selected from the group comprising ethylene glycol, propylene glycol or glycerol. Preferably the anti - freeze agent is Propylene glycol. The antifreezing agent may be present in an amount ranging from 5-10% w/w of the composition. Preferably, the amount of anti-freezing agent is 6% w/w of the composition.
The dispersing agent may be selected from the group comprising sodium lignosulphonates, sodium naphthalene sulphonate- formaldehyde condensates, aliphatic alcohol ethoxylates, tristyrylphenol ethoxylates and esters, ethylene oxide/propylene oxide block copolymers. Preferably, the dispersing agent is tri steryl phenol ethoxylate potassium salt, amine salt of poly aryl phenyl ether phosphate or combinations thereof. The dispersing agent may be present in an amount ranging from 3-8% w/w of the composition. Preferably, the amount of dispersing agent is 5% w/w of the composition.
The defoamer or an antifoaming agent may be selected from the group comprising silicone emulsions, long-chain alcohols, fatty acids, organ fluorine compounds. Preferably, the defoamer or the antifoaming agent is silicon based.The said defoamer or antifoaming agent may be present in an amount ranging from 0.2-1 % w/w of the composition. Preferably, the amount of deformer or the antifoaming agent is 0.5% w/w of the composition.
The present composition comprises one or more thickeners. Preferably, the thickener is Xanthan gum. Said thickener is added as per the specific requirements in the composition. Preferably, the amount of thickener is 0.05% w/w of the composition.
The present composition comprises one or more anti-bacterials or biocides.The antibacterial or the biocide may be present in an amount ranging from 0.1-0.5% of the composition.

Preferable, the amount of anti-bacterial or biocide is 0.2% w/w of the composition.
In another embodiment the present invention disclosed a process of preparing a suspension concentrate of the present invention.
The process of preparing the suspension concentrate comprises the following steps:
i. Diluting and solubilising a mixture surfactants and antifreeze agents in
water to obtain a solution, ii. Addition of defoaming agent to the solution of step (i) followed addition
of Pymetrozine technical to obtain a homogenous mixture; iii. Addition of Nitenpyram technical and Carbendazim technical and the
adjuvants to obtain a homogenous mass; iv. Size reduction of the homogenous mass of step (iv) to obtain mean particle
size of less than 5 micron; v. Preparation of an aqueous gum solution of 2% gum solution; optionally
containing formaldehyde; vi. Addition of the aqueous gum solution of step (v) to the homogenous mass
of step (iv) to obtain a suspension concentrate.
It has been observed that the composition of the present invention is synergistic and possesses enhanced agricultural effect as suspension concentrate.
In another embodiment the pesticidal combination and composition of present invention discloses a simultaneous, effective control of brown plant hopper (BPH) and brown spot disease in paddy crops.
Plant hoppers are important pests of agricultural crops. For instance, brown plant hopper (BPH) Nilaparvata lugens is a plant hopper species that feeds on rice plants (Oryza sativa L). High population of plant hoppers cause leaves to initially turn orange-yellow before becoming brown and dry and this is a condition called "hopper bum" that kills the plant. BPH can also transmit Rice ragged stunt virus and Rice grassy stunt virus from one plant to another. Plant hoppers can be a serious problem in rain-fed and in irrigated

wetland environments. It also occurs in areas with continuous submerged conditions in the field, high shade and humidity.
Brown spot a largely ignored fungal disease, is one of the most common and most damaging rice diseases. It infects the coleoptile, leaves, leaf sheath, panicle branches, glumes and spikelets. Its most observable damage is the numerous big yellowish-brownish spots on the leaves which can kill the whole leaf. Brown spot can occur at all crop stages, but the infection is most critical during maximum tillering up to the ripening stages of the crop. It causes both quality and quantity losses. The disease easily develops in areas with high relative humidity (86-100%) and a temperature between 16 and 36°C.
Unfortunately, similar weather conditions favour the development of both the spot disease and plant hopper insect attack. Humid conditions are especially congenial for the insect pests like Brown plant hopper and infestation by fungi and development of brown spot disease. The present invention therefore is useful in such conditions, where there is simultaneous spot disease and plant hopper insect attack.
EXAMPLES:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
Example 1: Preparation of the combination of present invention:
The present combination was prepared by undertaking the following steps:
1) The mixture of surfactants & anti freezing agents were first diluted in demineralised water, and solubilised by high speed mixer, defoamer was then added followed by addition of the Pymetrozine technical, this was mixed to obtain a homogenous mixture and then Nitenpyram technical was added, and then Carbendazim technical was added and rest of the adjuvants were mixed to obtain a homogenous mass.
2) The above mixed mass was ground in a sand mill. Grinding was carried out until a mean particle size of <5 micron was obtained.

3) 2% gum solution was prepared by taking 10 gm DM water, adding 0.5 gm of formaldehyde sol. then adding0.20gm of xanthan gum under slow stirring.
4) The 2% solution of xanthan gum obtained above was added to the homogenous mass obtained in step 2 under slow stirring.
5) Quality parameters of the final mix were checked.
Example 2; Composition of the Present Invention;
An exemplification of the composition of the present invention is at Table 2(a) and Table 2(b). Various parameters of the composition containing the combination of the present invention are tested by standard methods and set out at Table 2(c).
Table 2 (a); Chemical combination of the present invention

Ingredient % combination m/m
Pymetrozine technical (Basis of 100%) 17.00% m/m
Nitenpyram technical (Basis of 100%) 8.00% m/m
Carbendazim technical (Basis of 100%) 21.00% m/m
Anti-freezing agent (propylene glycol) 6.00% m/m
Dispersing agent (tri steryl phenol ethoxylate 5.00% m/m
Wetting agent (amine salt of poly aryl phenyl ether 3.00% m/m
Defoamer (silicon based) 0.5 % m/m
Anti-bacterial or Biocide (Formaldehyde) 0.20 m/m
Thickener (Xanthan gum) 0.05 m/m
D.M. water Q.S.
Table 2(b); Chemical combination of the present invention

Ingredients % combination m/m
Pymetrozine technical (Basis of 100%) 1-70 % m/m
Nitenpyram technical (Basis of 100%) 1-30% m/m
Carbendazim technical (Basis of 100%) 1-60% m/m
Anti-freezing agent (propylene glycol) 5-10% m/m
Dispersing agent (tri steryl phenol ethoxylate 3-8% m/m
Wetting agent (amine salt of poly aryl phenyl 2-5% m/m
Defoamer (silicon based) 0.2-1% m/m
Anti-bacterial or Biocide (Formaldehyde) 0.1-0.5%m/m
Thickener (Xanthan gum) 00.05 m/m
D.M. water Q.S.

Table Kc^: Test parameters

Form Material shall be in the form of free-flowing
Pourability Residue shall not be more than 5% w/w.
Spontaneity of dispersion Shall not be less than 50% w/w
Wet sieve test 98% min (passes through 45 micron sieve).
Persistence of Foam Persistence of foam shall not be more than 60 ml in 12
A.I. content Pymetrozine 17% + Nitenpyram 8% + Carbendazim
Suspensibility Not less than 80% w/w
pH of 1% aq. Solution 6.0-8.0
HST (Heat Stability Test) Take 100ml sample & keep it in 54°C±2 for 14 days.
Cold Test Take 100ml sample & keep it in 5°C±2 for 14 days
Example 3; Bioefficacv tests of the present combination;
The bioefficacy of the present insecticidal-fungicidal combination was tested against the insect pest -BPH (Brown plant hopper) and brown spot disease in Paddy crop. The field trials were conducted during the Kharif season 2016-17 in Rudrapur, Uttarakhand of India. The trials were conducted in random block design in a plot size of 30 sq. m. at a temperature of 35.5°C and at a relative humidity of 80%. A total of nine treatments in three replications were performed following the foliar over the top application of the composition.
Measured quantity of the chemical was added to the required volume of water @150 lit. /ac for spraying. The spray tank was filled with half the quantity of clean required volume of water and then the chemical measured according to the dose was added followed by the rest half quantity of water. The solution was stirred well, and thorough coverage were ensured. The results are presented at Table 3.

Table 3; below provides the treatment details of the trials conducted on paddv with the present composition

Treatments Treatment details Dose gm a.i./acre Ratio
T-l Untreated -
T-2 Nitenpyram 10 SL 30 g
T-3 Pymetrozine 50 WG 60 g
T-4 Carbendazim 50 WP 75 g
T-5 CCP-81721 SC 24+51+63 1: 2:2.62
T-6 CCP-81721 SC 26+55.25+68.25 1: 2:2.62
T-7 CCP-81721 SC 28+59.5+73.5 1: 2:2.62
T-8 CCP-81620 SC 24+48 + 62.4 1 :2:2.6
T-9 CCP-81620 SC 26+52 + 67.6 1 :2:2.6
T-10 CCP-81620 SC 28+56+72.8 1 :2:2.6
T-ll CCP-82420 SC 24+72+62.4 1 :3:2.6
T-12 CCP-82420 SC 26+78+67.6 1 :3:2.6
T-13 CCP-82420 SC 28+84+72.8 1 :3:2.6
T-14 CCP-81624 SC 24+48+72 1:2:3
T-15 CCP-81624 SC 26+52+78 1:2:3
T-16 CCP-81624 SC 28+56+84 1:2:3
T-17 CCP-81623 SC 24+50.4+62.4 1:2.1:2.6
T-18 CCP-81623 SC 26+54.6+67.6 1:2.1:2.6
T-19 CCP-81623 SC 28+58.8+72.8 1:2.1:2.6
T-20 CCP-81820 SC 24+36+62.4 1:2.25:2.6
T-21 CCP-81820 SC 26+58.5+67.6 1:2.25:2.6
T-22 CCP-81820 SC 28+63+72.8 1:2.25:2.6
T-23 CCP-82020 SC 24+60+62.4 1:2.5:2.6
T-24 CCP-82020 SC 26+65+67.6 1:2.5:2.6
T-25 CCP-82020 SC 28+70+72.8 1:2.5:2.6
3(a) Evaluation of bioefficacv; Target insect pest; Brown plant hopper (BPH) in paddv
The number of live BPH were counted from 10 randomly selected hills/replication before application (pre-count) and at 1, 3, 5, 7, 10 days after application and at 5 days interval thereafter till re-infestation. The percent control of BPH Population was calculated based on the no. of live thrips population. Formula are given below.
Percent control = ((C-T)/C) x 100
Where C =No. of BPH (Both Nymph & Adult) population in control plot T = No. of BPH (Both Nymph & Adult) population in treated plot

It is seen that the combination of the present invention has enhanced efficacy in comparison to the individual components.
ii. Target fungal pest; Helminthosporium orvzae (Brown spot of rice)
Observation of disease symptoms were taken before every spray and 15 days after last application. Disease assessment was done as per the rating scale of 0 to 9 given by Mayee & Datar (Phyto Pathometery, 1986) on the basis of affected leaf area. The rating scale is shown below:

1 No Incidence
2 Less than 1%
3 1-3%
4 4-5%
5 11-15%
6 16-25%
7 26-50%
8 51-75%
9 76-100%
The disease index was calculated as per the formula below:
Sum of all the numerical disease rating X 100
% Disease Index =
(PDI) Total no. of plants (10) X Maximum disease grade in scale (9)
It can be seen that the disease index of the present invention is considerably reduced on administration of a combination of the ingredient in comparison to the individual components.
hi. Evaluation of phvtotoxicitv
Visual observations were recorded at 3, 7 and 10 days after application. The parameters observed were leaf injury on tip/surface, necrosis, vein clearing, epinasty, hyponasty and wilting. For the rating on phytotoxicity the core Scale of 0 - 10 was followed for

leaf injury on tips/surface. The results are presented at Table 4. Table 4; Phytotoxicity symptoms scoring and rating for leaf injury on tip/surface

Leaf injury on tips/surface Rating
0% 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10
Further evaluation were done and the results are presented below at Tables to 5 to 9.

Table 5: Bioefficacy of Trimix of Novel insecticide Nitenpyram + Pymetrozine + Carbendazim against BPH (After First Application)

Treatment Treatment details Dose gm a.i./acre Pre Treat 1DA1A % Control 5DA1A % Control 10 DA1A % Control
T-l Untreated - 42.7 44.6 - 46.7 - 48.4 -
T-2 Nitenpyram 10 SL 30 g 43.2 19.7 55.8 9.3 80.1 15.5 68
T-3 Pymetrozine 50 WG 60 g 45.4 20.3 33.9 10.6 77.3 15.1 68.8
T-4 Carbendazim 50 WP 75 g 45.6 29.5 54.5 30.4 34.9 34.8 28.1
T-5 CCP-81721 SC (1:2:2.62) 24+51+63 46 18.1 59.4 8.8 81.2 10.3 78.7
T-6 CCP-81721 SC (1:2:2.62) 26+55.25+68.25 39.7 18.3 59 7.9 83.1 8.7 82.0
T-7 CCP-81721 SC (1:2:2.62) 28+59.5+73.5 41.6 17.7 60.3 6.5 86.1 7.1 85.3
T-8 CCP-81620 SC (1:2:2.6) 24+48 + 62.4 36.5 19.2 56.95 9.5 79.65 11.3 76.65
T-9 CCP-81620 SC (1:2:2.6) 26+52 + 67.6 40.5 18.8 57.84 9.2 80.29 10.5 78.30
T-10 CCP-81620 SC (1:2:2.6) 28+56+72.8 32 18.5 58.52 8.7 81.37 9.5 80.37
T-ll CCP-82420 SC (1:3:2.6) 24+72+62.4 37 17.9 59.86 8.0 82.86 8.5 82.43
T-12 CCP-82420 SC (1:3:2.6) 26+78+67.6 38 17.5 65.24 7.9 83.08 8.1 83.26
T-13 CCP-82420 SC (1:3:2.6) 28+84+72.8 40 17.2 61.43 7.5 83.94 7.9 83.67
T-14 CCP-81624 SC (1:2:3) 24+48+72 42 20.5 54.03 10.5 77.51 12.4 74.38
T-15 CCP-81624 SC (1:2:3) 26+52+78 44 19.8 55.6 9.8 79.01 11.8 75.61
T-16 CCP-81624 SC (1:2:3) 28+56+84 46 18.5 58.5 9 80.72 11 77.27
T-17 CCP-81623 SC (1:2.1:2.6) 24+50.4+62.4 43 21.4 52.01 11 76.44 10 79.33
T-18 CCP-81623 SC (1:2.1:2.6) 26+54.6+67.6 47 20.8 53.36 9.5 79.65 9.5 80.37
T-19 CCP-81623 SC (1:2.1:2.6) 28+58.8+72.8 49.5 19.2 56.95 9 80.72 9.2 80.99
T-20 CCP-81820 SC (1:2.25:2.6) 24+36+62.4 43 20.5 54.03 9.5 79.65 10 79.33
T-21 CCP-81820 SC (1:2.25:2.6) 26+58.5+67.6 47 19.8 55.6 9.2 80.29 9.7 79.95
T-22 CCP-81820 SC (1:2.25:2.6) 28+63+72.8 32.2 19.6 56.05 8.5 81.79 9.0 81.40
T-23 CCP-82020 SC (1:2.5:2.6) 24+60+62.4 30 19.0 57.39 9.8 79.01 12 75.20
T-24 CCP-82020 SC (1:2.5:2.6) 26+65+67.6 37.5 18.8 57.84 9.5 79.65 8.8 81.81
T-25 CCP-82020 SC (1:2.5:2.6) 28+70+72.8 32.5 18.5 58.52 9 80.72 7.8 83.88
15

Table 6: Bioefficacv of Trimix of Novel insecticide Nitenpyram + Pymetrozine + Carbendazim against BPH (After Second Application)

Treat Treatment details Dose gm a.i./acre Pre Treat 1DA2A % Control 5DA2A % Control 10 DA2A % Control
T-l Untreated - 48.4 51 - 55.7 - 65 -
T-2 Nitenpyram 10 SL 30 g 15.5 11.5 77.45 10.6 80.96 12.5 80.76
T-3 Pymetrozine 50 WG 60 g 15.1 12.3 75.88 11.2 79.89 13.2 79.69
T-4 Carbendazim 50 WP 75 g 34.8 40 21.56 44 21.00 50 23.07
T-5 CCP-81721SC (1:2:2.62) 24+51+63 10.3 9.5 81.37 9.0 83.84 9.5 85.38
T-6 CCP-81721 SC (1:2:2.62) 26+55.25+68.25 8.7 8 84.31 7.5 86.53 7.3 88.76
T-7 CCP-81721 SC (1:2:2.62) 28+59.5+73.5 7.1 6.5 87.25 6.0 89.22 6.2 90.46
T-8 CCP-81620 SC (1:2:2.6) 24+48 + 62.4 11.3 10 80.39 9.5 82.94 10 84.61
T-9 CCP-81620 SC (1:2:2.6) 26+52 + 67.6 10.5 9.8 80.78 9.1 83.66 9.5 85.38
T-10 CCP-81620 SC (1:2:2.6) 28+56+72.8 9.5 9 82.35 8.5 84.73 9.1 86.00
T-ll CCP-82420 SC (1:3:2.6) 24+72+62.4 8.5 8 84.31 7.6 86.35 8.9 86.30
T-12 CCP-82420 SC (1:3:2.6) 26+78+67.6 8.1 7.9 84.50 7.3 86.89 8.5 86.92
T-13 CCP-82420 SC (1:3:2.6) 28+84+72.8 7.9 7.3 85.68 6.8 87.79 8.3 87.23
T-14 CCP-81624 SC (1:2:3) 24+48+72 12.4 11.5 77.45 10.5 81.14 12 81.53
T-15 CCP-81624 SC (1:2:3) 26+52+78 11.8 10 80.39 9.8 82.40 11.5 82.30
T-16 CCP-81624 SC (1:2:3) 28+56+84 11 10.5 79.41 9.5 82.94 11 83.07
T-17 CCP-81623 SC (1:2.1:2.6) 24+50.4+62.4 10 9.5 81.37 9.2 83.48 10.5 83.84
T-18 CCP-81623 SC (1:2.1:2.6) 26+54.6+67.6 9.5 9.2 81.96 8.5 84.73 10 84.61
T-19 CCP-81623 SC (1:2.1:2.6) 28+58.8+72.8 9.2 9.0 82.35 8.2 85.27 9.8 84.92
T-20 CCP-81820 SC (1:2.25:2.6) 24+36+62.4 10 9.5 81.37 9.0 83.84 11.5 82.30
T-21 CCP-81820 SC (1:2.25:2.6) 26+58.5+67.6 9.7 9.2 81.96 8.7 84.38 10.5 83.84
T-22 CCP-81820 SC (1:2.25:2.6) 28+63+72.8 9.0 8.5 83.33 8.3 85.09 9.25 85.76
T-23 CCP-82020 SC (1:2.5:2.6) 24+60+62.4 12 10 80.39 9.8 82.40 9.8 84.92
T-24 CCP-82020 SC (1:2.5:2.6) 26+65+67.6 8.8 8.5 83.33 9.5 82.94 9.2 85.84
T-25 CCP-82020 SC (1:2.5:2.6) 28+70+72.8 7.8 8.2 83.92 9.2 83.48 8.7 86.61
16

Table 7: Bioefficacv of Nitenpyram + Pymetrozine + Carbendazim against Brown spot of rice

Treat. Treatment details Dose gm a.i./acre PDI (25DA2A) % Control PDI 50 DA2A % Control
T-l Untreated - 30.25 - 40.5 -
T-2 Nitenpyram 10 SL 30 g 25.5 15.70 32 20.98
T-3 Pymetrozine 50 WG 60 g 23.5 22.31 34.5 14.81
T-4 Carbendazim 50 WP 75 g 4.50 85.12 6.5 83.95
T-5 CCP-81721 SC (1:2:2.62) 24+51+63 6.20 79.50 7.5 81.48
T-6 CCP-81721 SC (1:2:2.62) 26+55.25+68.25 5.00 83.47 7.1 82.46
T-7 CCP-81721 SC (1:2:2.62) 28+59.5+73.5 4.25 85.95 6.9 82.96
T-8 CCP-81620 SC (1:2:2.6) 24+48 + 62.4 7.5 75.20 8.0 80.24
T-9 CCP-81620 SC (1:2:2.6) 26+52 + 67.6 6.8 77.52 7.5 81.48
T-10 CCP-81620 SC (1:2:2.6) 28+56+72.8 6.3 79.17 7.2 82.22
T-ll CCP-82420 SC (1:3:2.6) 24+72+62.4 7.9 73.88 8.5 79.01
T-12 CCP-82420 SC (1:3:2.6) 26+78+67.6 7.2 76.19 7.8 80.74
T-13 CCP-82420 SC (1:3:2.6) 28+84+72.8 6.6 78.18 7.0 82.71
T-14 CCP-81624 SC (1:2:3) 24+48+72 7.1 76.52 7.6 81.23
T-15 CCP-81624 SC (1:2:3) 26+52+78 6.6 78.18 7.3 81.97
T-16 CCP-81624 SC (1:2:3) 28+56+84 6.0 80.16 6.9 82.96
T-17 CCP-81623 SC (1:2.1:2.6) 24+50.4+62.4 6.9 77.19 7.2 82.22
T-18 CCP-81623 SC (1:2.1:2.6) 26+54.6+67.6 6.2 79.50 7.0 82.71
T-19 CCP-81623 SC (1:2.1:2.6) 28+58.8+72.8 6.0 80.16 6.9 82.96
T-20 CCP-81820SC (1:2.25:2.6) 24+36+62.4 8.0 73.55 8.5 79.01
T-21 CCP-81820SC (1:2.25:2.6) 26+58.5+67.6 7.5 75.20 8.2 79.75
T-22 CCP-81820SC (1:2.25:2.6) 28+63+72.8 7.2 76.19 8.0 80.24
T-23 CCP-82020 SC (1:2.5:2.6) 24+60+62.4 9.5 68.59 10 75.30
T-24 CCP-82020 SC (1:2.5:2.6) 26+65+67.6 8.0 73.55 9.8 75.80
T-25 CCP-82020 SC (1:2.5:2.6) 28+70+72.8 7.6 74.87 9.2 77.28
17

Table 8: Phytotoxicity of Nitenpyram + Pymetrozine + Carbendazim on Rice (After First Application)

Treat. Treatment details Dose 12hrs 1DAA 3DAA 5DAA 7DAA 10DAA
T-l Untreated - 0 0 0 0 0 0
T-2 Nitenpyram 10 SL 30 g 0 0 0 0 0 0
T-3 Pymetrozine 50 WG 60 g 0 0 0 0 0 0
T-4 Carbendazim 50 WP 75 g 0 0 0 0 0 0
T-5 CCP-81721 SC (1:2:2.62) 24+51+63 0 0 0 0 0 0
T-6 CCP-81721 SC (1:2:2.62) 26+55.25+68.25 0 0 0 0 0 0
T-7 CCP-81721 SC (1:2:2.62) 28+59.5+73.5 0 0 0 0 0 0
T-8 CCP-81620 SC (1:2:2.6) 24+48 + 62.4 0 0 0 0 0 0
T-9 CCP-81620 SC (1:2:2.6) 26+52 + 67.6 0 0 0 0 0 0
T-10 CCP-81620 SC (1:2:2.6) 28+56+72.8 0 0 0 0 0 0
T-ll CCP-82420 SC (1:3:2.6) 24+72+62.4 0 0 0 0 0 0
T-12 CCP-82420 SC (1:3:2.6) 26+78+67.6 0 0 0 0 0 0
T-13 CCP-82420 SC (1:3:2.6) 28+84+72.8 0 0 0 0 0 0
T-14 CCP-81624 SC (1:2:3) 24+48+72 0 0 0 0 0 0
T-15 CCP-81624 SC (1:2:3) 26+52+78 0 0 0 0 0 0
T-16 CCP-81624 SC (1:2:3) 28+56+84 0 0 0 0 0 0
T-17 CCP-81623 SC (1:2.1:2.6) 24+50.4+62.4 0 0 0 0 0 0
T-18 CCP-81623 SC (1:2.1:2.6) 26+54.6+67.6 0 0 0 0 0 0
T-19 CCP-81623 SC (1:2.1:2.6) 28+58.8+72.8 0 0 0 0 0 0
T-20 CCP-81820 SC (1:2.25:2.6) 24+36+62.4 0 0 0 0 0 0
T-21 CCP-81820 SC (1:2.25:2.6) 26+58.5+67.6 0 0 0 0 0 0
T-22 CCP-81820 SC (1:2.25:2.6) 28+63+72.8 0 0 0 0 0 0
T-23 CCP-82020 SC (1:2.5:2.6) 24+60+62.4 0 0 0 0 0 0
T-24 CCP-82020 SC (1:2.5:2.6) 26+65+67.6 0 0 0 0 0 0
T-25 CCP-82020 SC (1:2.5:2.6) 28+70+72.8 0 0 0 0 0 0

Table 9: Phytotoxicity of Nitenpyram + Pymetrozine + Carbendazim on Rice (After Second Application)

Treat. Treatment details Dose gm a.i./acre 12hrs 1DAA 3DAA 5DAA 7DAA 10DAA
T-l Untreated - 0 0 0 0 0 0
T-2 Nitenpyram 10 SL 30 g 0 0 0 0 0 0
T-3 Pymetrozine 50 WG 60 g 0 0 0 0 0 0
T-4 Carbendazim 50 WP 75 g 0 0 0 0 0 0
T-5 CCP-81721 SC (1:2:2.62) 24+51+63 0 0 0 0 0 0
T-6 CCP-81721 SC (1:2:2.62) 26+55.25+68.25 0 0 0 0 0 0
T-7 CCP-81721 SC (1:2:2.62) 28+59.5+73.5 0 0 0 0 0 0
T-8 CCP-81620 SC (1:2:2.6) 24+48 + 62.4 0 0 0 0 0 0
T-9 CCP-81620 SC (1:2:2.6) 26+52 + 67.6 0 0 0 0 0 0
T-10 CCP-81620 SC (1:2:2.6) 28+56+72.8 0 0 0 0 0 0
T-ll CCP-82420 SC (1:3:2.6) 24+72+62.4 0 0 0 0 0 0
T-12 CCP-82420 SC (1:3:2.6) 26+78+67.6 0 0 0 0 0 0
T-13 CCP-82420 SC (1:3:2.6) 28+84+72.8 0 0 0 0 0 0
T-14 CCP-81624 SC (1:2:3) 24+48+72 0 0 0 0 0 0
T-15 CCP-81624 SC (1:2:3) 26+52+78 0 0 0 0 0 0
T-16 CCP-81624 SC (1:2:3) 28+56+84 0 0 0 0 0 0
T-17 CCP-81623 SC (1:2.1:2.6) 24+50.4+62.4 0 0 0 0 0 0
T-18 CCP-81623 SC (1:2.1:2.6) 26+54.6+67.6 0 0 0 0 0 0
T-19 CCP-81623 SC (1:2.1:2.6) 28+58.8+72.8 0 0 0 0 0 0
T-20 CCP-81820 SC (1:2.25:2.6) 24+36+62.4 0 0 0 0 0 0
T-21 CCP-81820 SC (1:2.25:2.6) 26+58.5+67.6 0 0 0 0 0 0
T-22 CCP-81820 SC (1:2.25:2.6) 28+63+72.8 0 0 0 0 0 0
T-23 CCP-82020 SC (1:2.5:2.6) 24+60+62.4 0 0 0 0 0 0
T-24 CCP-82020 SC (1:2.5:2.6) 26+65+67.6 0 0 0 0 0 0
T-25 CCP-82020 SC (1:2.5:2.6) 28+70+72.8 0 0 0 0 0 0

Results:
Tables 5 and 6 above indicate that the maximum percent control of BPH population and minimum percent disease index (Table-7) of brown spot of rice was observed with composition at a ratio of 1:2:2.62 at a dosage of 26+55.25+68.25 gm active ingredients and 28+59.5+73.5 gm active ingredients of Nitenpyram, Pymetrozine and Carbendazim respectively per acre with minimum 2 applications.
Further, no phytotoxic symptoms as shown in (Table-8 & 9) were observed in Paddy crop.
Advantages
The present invention ensures uniform and homogeneous distribution of active ingredients and can be easily sprayed on paddy crops for effective control of brown plant hopper (BPH) and brown spot disease in paddy crops.

We Claim;

1.A synergistic pesticidal combination wherein said combination comprises:
a. At least one insecticide belonging to the group of neonicotinoids;
b. At least one insecticide belonging to the group of pyridines azomethines;
c. At least one fungicide belonging to the group of benzimidazole.
2. The synergistic pesticidal combination as claimed in claim 1, wherein the neonicotinoid is selected from the group comprising Acetamiprid, Clothianidin, Imidacloprid, Nitenpyram Nithiazine, Dinotefuran, Thiacloprid, Thiamethoxam, preferably the insecticide is Nitenpyram.
3. The synergistic pesticidal combination as claimed in claim 1, wherein the pyridine azomethine is pymetrozine.
4. The synergistic pesticidal combination as claimed in claim 1, wherein the benzimidazoles is selected from the group comprising Carbendazim, (MBC), thiophanate-methyl, thiabendazole, fuberidazole, Albendazole, Benomyl, Chlorfenazole, cypendazole, debacarb, mecarbinzid, Rabenzazole, more preferably carbendazim.
5. The synergistic pesticidal combination as claimed in claim 1, wherein said combination comprises:

a) Nitenpyram in the range of 1-30% w/w of the total weight, preferably 1-28% w/w, more preferably 1-25% w/w of the combination,
b) Pymetrozine in the range of 1-70% w/w, preferably 1-68% w/w, more preferably 1-65% w/w of the total weight of the combination.
c) Carbendazim in the range of 1-60% w/w, preferably 1-58% w/w, more preferably 1-55% w/w of the total weight of the combination.

6. The synergistic pesticidal combination as claimed in claim 1, wherein the ratio of combination is 1:1:1 to 1:3:3.
7. A pesticidal composition comprising the combination as claimed in claim 1, along with agriculturally acceptable excipients.
8. The pesticidal composition as claimed in claim 7, wherein said composition is formulated in a form selected from the group comprising suspension concentrates, highly concentrated aqueous suspensions, wet dispersible powders, water dispersible granules, dispersions and aqueous solutions, preferably a suspension concentrate.

9. The pesticidal composition as claimed in claim 7, wherein the agriculturally acceptable
excipients are selected from the group comprising anti-freezing agent, dispersing agent,
wetting agent, deformer, antibacterial, biocide, thickener and demineralized water.
10. The pesticidal composition as claimed in claim 9, wherein said antifreeze agent is selected from the group comprising ethylene glycol, propylene glycol and glycerol.
11. The pesticidal composition as claimed in claim 9, wherein said dispersing agent is selected from the group comprising sodium lignosulphonates, sodium naphthalene sulphonate- formaldehyde condensates, aliphatic alcohol ethoxylates, tristyrylphenol ethoxylates and esters and ethylene oxide/propylene oxide block copolymers.
12. The pesticidal composition as claimed in claim 9, wherein said antifoaming agent is selected from the group comprising silicone emulsions, long-chain alcohols, fatty acids, organ fluorine compounds.
13. The pesticidal composition as claimed in claim 9, wherein said thickener is Xanthan gum.
14. A process of preparing the synergistic pesticidal combination as claimed in claim 1, wherein said process comprises the following steps:
i. Diluting and solubilising a mixture surfactants and antifreeze agents in water to
obtain a solution, ii. Addition of defoaming agent to the solution of step (i) followed addition of
Pymetrozine technical to obtain a homogenous mixture; iii. Addition of Nitenpyram technical and Carbendazim technical and adjuvants to
obtain a homogenous mass; iv. Size reduction of the homogenous mass of step (iv) to obtain mean particle size
of less than 5 micron; v. Preparation of an aqueous gum solution of 2% gum solution; optionally
containing formaldehyde; vi. Addition of the aqueous gum solution of step (v) to the homogenous mass of step
(iv) to obtain a suspension concentrate.
15. The combination and composition as claimed in claim 1 and 7 for simultaneous, effective
control of brown plant hopper (BPH) and brown spot disease in paddy crops.