Description:FORM 2
THE PATENTS ACT 1970
(39 OF 1970)
&
The Patent Rules, 2003

COMPLETE SPECIFICATION
(See section 10 and Rules 13)

TITLE OF THE INVENTION
A photocatalytic composition to make biofilm and process for preparation thereof

APPLICANTS:
(a) ADVISENSE (OPC) PVT. LTD
(b) An Indian small entity company
(c) 1136/32, Kali Kutir, Arya Nagar,
Ajmer, Rajasthan -305001

PREAMBLE TO THE DESCRIPTION
The following specification describes the invention and the manner in which it is to be performed.

The following specification in respect of a patent of addition is sumitted in reference to the main patent application no. 202011031905, filed on 25 July 2020, title as ‘A Novel BioFilm as a Photo Catalyst Biological Protective Shieldsuper”
FIELD OF THE INVENTION
This invention relates to a photocatalytic composition to make biofilm and process for preparation thereof. Particularly this invention relates to a photocatalytic composition to make biofilm to use for sanitization and disinfection and process for preparation thereof.
BACKGROUND OF THE INVENTION
In the biology, biofilm research is a dynamic field. Bale silage preserves forage crops using agricultural film, allowing wet hay baling and outdoor storage, reducing barn space needs. This airtight system enables fermentation, enhancing nutrient content by converting sugars to lactic acid. Suitable crops include grasses, clovers, alfalfa, vetches, oats, rye, and maize. Removing the film before feeding livestock is labor-intensive and hazardous if ingested. Non-biodegradable wraps often end up in landfills or are illegally burned, releasing toxic chemicals. Recycling is difficult due to contamination, and burial generates toxic leachate. There is a critical need for a functional, durable, and easily disposable biofilm.
Canada patent, publication no. CA2933770A1 discloses a bio-polymer agriculture film comprising starch, acid, glycerol, and biomass fiber. The biomass fiber component encompasses a variety of materials including corn, sugarcane bagasse, or hemp, contributing to structural reinforcement and ecological viability. The film's thickness ranges from 10 to 50 microns, ensuring requisite flexibility and durability for agricultural applications. The manufacturing protocol involves heating a mixture of starch, acid, and glycerol to form the bio-polymer, followed by the inclusion of biomass fiber and subsequent film formation. Additional thermal treatment of the biomass fiber with the bio-polymer further optimizes material properties.
Canada patent, publication no. CN108641124A discloses the biological base film of a kind of high content of biological carbon content, low-density, which is characterized in that the biology base film includes with the following group Part:Biomass polyolefin 10~80%, thermoplastic starch 10~40%, petroleum base polyolefin 0~60%, inorganic assistant 3~ 15%
Worldwide patent, publication no. WO2011140496A1 discloses biaxially oriented bio-based polyolefin films and laminates comprising a high radiocarbon content, with values of at least 53 pMC. Comprising biaxially oriented layers of bio-based polyolefin resin, the film can incorporate crystalline isotactic propylene homopolymer or bio-based ethylene homopolymer. Co-extrusion techniques facilitate the incorporation of additional layers, potentially comprising bio-based propylene or ethylene copolymers. The lamination process involves joining biaxially oriented films with bio-based low-density polyethylene adhesive
There are disadvantages associated with the conventional composition to make biofilm. One of the disadvantages is that the conventional composition comprises bio-based materials which is inefficient for sanitization and disinfection of microbes.
Another disadvantage associated with the conventional composition to make biofilm is that the improper use of bleach reduces its effectiveness in disinfection.
Yet another disadvantage associated with the conventional composition to make biofilm is that bleach irritates mucous membranes, the skin and the airways; decomposes under heat and light; and reacts easily with other chemicals.
Still another disadvantage associated with the conventional composition to make biofilm is that the conventional composition do not have a photocatalytic properties.
OBJECTIVE OF THE INVENTION
Therefore, an objective of the present invention is to provide a photocatalytic composition to make biofilm which is efficient for sanitization and disinfection of microbes.
Another objective of the present invention is to provide a photocatalytic composition to make biofilm which extended protection over 5 days, equivalent to 120 hours, even under minimal sunlight exposure for up to 90 days.
Yet another objective of the present invention is to provide a photocatalytic composition to make biofilm which offer an environmentally benign solution: non-detrimental upon disposal, non-toxic, and posing no harm to users or patients.
Still, another objective of the present invention is to provide a photocatalytic composition to make biofilm which offer an economically cost-effective method , and prevents the release of heavy metals, thereby limiting their absorption, while promoting soil buffering and reduced compaction.
SUMMARY OF THE INVENTION
According to the invention, there is provided a photocatalyst composition to make a biofilm comprising 48 – 62 % by weight Titanium dioxide (TiO2); 27 – 29 % by weight Silicon dioxide (SiO2); 1 - 3 % by weight Sodium percarbonate(Na2H3CO6); 1 – 3 % by weight Sodium Bromide (NaBr); 10 – 12 % by weight Zeolite (MxAlxSi1-xO2·yH2O); 1 – 3 % by weight Lime (Ca(OH)2.
Further, according to the invention, there is provided a process for preparation of photocatalyst composition to make biofilm comprises mixing 48 – 62 % by weight Titanium dioxide (TiO2); 27 – 29 % by weight Silicon dioxide (SiO2) and calcinating the same at 500 - 600 OC for 55 – 65 minutes, treating the calcinated mixured with a solution of NaBr and drying the same at 180 – 220 °C to modify the surface of the particles of the mixture to activate absorption of light through the surface, adding 1 – 3 % by weight Lime (Ca(OH)2, 1 - 3 % by weight Sodium percarbonate(Na2H3CO6), and 10 – 12 % by weight Zeolite (MxAlxSi1-xO2·yH2O) into treated mixture and mixing the same for 100 – 140 minutes to obtain photocatalyst composition to make a biofilm.
BRIEF DESCRIPTION OF THE DRAWINGS
A photocatalytic composition to make biofilm and process for preparation thereof, according to a preferred embodiment of the present invention is herein described and illustrated in the accompanying drawings and examples wherein;
Fig.1: Graphical representation of microbiological parameters – anti-microbial efficacy,
Fig.2: Graphical representation of microbiological parameter – antiviral efficacy,
Fig.3: Graphical representation of Microbiological Parameter – anti Microbial Efficacy (Longevity of Coverage),
Fig.4: Graphical representation of Microbiological Parameter – anti Microbial Under Sunlight: (Sanitization Fogging),
Fig.5: Graphical representation of Microbiological Parameter – anti Microbial in dark mode (sterilization – swab test).

DETAILED DESCRIPTION OF THE INVENTION
A photocatalytic composition to make biofilm and process for preparation thereof is herein described with numerous specific details so as to provide a complete understanding of the invention. However, these specific details are exemplary details and should not be treated as the limitation to the scope of the invention. Throughout this specification the word “comprise” or variations such as “comprises or comprising”, will be understood to imply the inclusions of a stated element, integer or step, or group of elements, integers or steps, but not the exclusions of any other element, integer or step or group of elements, integers or steps.
The photocatalytic composition to make biofilm comprises a photocatalyst composition to make a biofilm comprises 48 – 62 % by weight Titanium dioxide (TiO2); 27 – 29 % by weight Silicon dioxide (SiO2); 1 - 3 % by weight Sodium percarbonate(Na2H3CO6); 1 – 3 % by weight Sodium Bromide (NaBr); 10 – 12 % by weight Zeolite (MxAlxSi1-xO2·yH2O); 1 – 3 % by weight Lime (Ca(OH)2.
The Titanium dioxide (TiO2) is prepared by mixing of 80 – 90 % by weight Anatase; 10 - 20 % by weight Rutile.
A process for preparation of photocatalyst composition to make biofilm comprises 48 – 62 % by weight Titanium dioxide (TiO2); 27 – 29 % by weight Silicon dioxide (SiO2) are mixed with eachother and calcinated the same at 500 - 600 OC for 55 – 65 minutes. The calcinated mixured is treated with 1 – 3 % by weight of a solution of NaBr and dried the same at 180 – 220 °C to modify the surface of the particles of the mixture to activate absorption of light through the surface. 1 – 3 % by weight Lime (Ca(OH)2, 1 - 3 % by weight dissolved Sodium percarbonate(Na2H3CO6), and 10 – 12 % by weight Zeolite (MxAlxSi1-xO2·yH2O) are added into treated mixture and again mixed the same for 100 – 140 minutes to obtain photocatalyst composition to make a biofilm.
The sodium percarbonate is dissolved in water, for example, 1 - 3 gram dissolved in 1000 ml water.
EXAMPLE -1
53 gram Titanium dioxide (TiO2); 28 gram Silicon dioxide (SiO2) were mixed with eachother and calcinated the same at 550 OC for 60 minutes. The calcinated mixured was treated with 3 gram solution of NaBr and dried the same at 200 °C to modify the surface of the particles of the mixture to activate absorption of light through the surface. 2.5 gram Lime (Ca(OH)2, 2.5 gram dissolved Sodium percarbonate(Na2H3CO6), and 11 gram Zeolite (MxAlxSi1-xO2·yH2O) were added into treated mixture and again mixed the same for to obtain photocatalyst composition to make a biofilm.
EXAMPLE -2
58 gram Titanium dioxide (TiO2); 27 gram Silicon dioxide (SiO2) were mixed with eachother and calcinated the same at 520 OC for 62 minutes. The calcinated mixured was treated with 2 gram solution of NaBr and dried the same at 210 °C to modify the surface of the particles of the mixture to activate absorption of light through the surface 2 gram Lime (Ca(OH)2, 2 gram dissolved Sodium percarbonate(Na2H3CO6), and 10 gram Zeolite (MxAlxSi1-xO2·yH2O) were added into treated mixture and again mixed the same for to obtain photocatalyst composition to make a biofilm.
The present invented photocatalyst composition was tested by the multiple labs regarding anti-microbial efficacy, disinfection technique, sanitization techinque etc. The tested result are mentioned as follows
A lab results of anti-microbial efficacy, disinfection technique, sanitization technique tested with approx. 50kg sample which reveals the following results
Table 1
A lab results of anti-viral efficacy, disinfection technique, sanitization technique tested with approx. 50kg sample which reveals the following results

Table 2
A lab results of anti-microbial efficacy tested under visible ligh and sunlight with approx. 50kg sample which reveals the following results

Table 3
A lab results of anti-microbial efficacy tested in dark environment with approx. 50kg sample which reveals the following results

Table 4

Certain features of the invention have been described with reference to the example embodiments. However, the description is not intended to be construed in a limiting sense. Various modifications of the example embodiments as well as other embodiments of the invention, which are apparent to the persons skilled in the art to which the invention pertains, are deemed to lie within the spirit and scope of the invention.
, Claims:WE CLAIM:
1. A photocatalyst composition to make a biofilm comprising 48 – 62 % by weight Titanium dioxide (TiO2); 27 – 29 % by weight Silicon dioxide (SiO2); 1 - 3 % by weight Sodium percarbonate(Na2H3CO6); 1 – 3 % by weight Sodium Bromide (NaBr); 10 – 12 % by weight Zeolite (MxAlxSi1-xO2·yH2O); 1 – 3 % by weight Lime (Ca(OH)2.
2. The photocatalyst composition to make biofilm as claimed in claim 1, wherein the Titanium dioxide (TiO2) is a mixer of 80 – 90 % by weight Anatase; 10 - 20 % by weight Rutile.
3. A process for preparation of photocatalyst composition to make biofilm comprising
I. Mixing 48 – 62 % by weight Titanium dioxide (TiO2); 27 – 29 % by weight Silicon dioxide (SiO2) and calcinating the same at 500 - 600 OC for 55 – 65 minutes,
II. Treating the calcinated mixured with a solution of NaBr and drying the same at 180 – 220 °C to modify the surface of the particles of the mixture to activate absorption of light through the surface,
III. Adding 1 – 3 % by weight Lime (Ca(OH)2, 1 - 3 % by weight dissolved Sodium percarbonate(Na2H3CO6), and 10 – 12 % by weight Zeolite (MxAlxSi1-xO2·yH2O) into treated mixture and mixing the same for 100 – 140 minutes to obtain photocatalyst composition to make a biofilm.
4. The process for preparation of photocatalyst composition to make biofilm as claimed in claim 3, wherein 1 – 3 % by weight of solution of NaBr is used to treat the mixture.
Dated this 27th day of June 2024

R. P. Yadav
IN/PA – 954
of sr4ipr Partners
Applicant’s Attorney