A Process For Characterization Of A Malarial Molecule Hmgb 1 For

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A Process For Characterization Of A Malarial Molecule Hmgb 1 For Developing An Anti Disease Vaccine.

Abstract: The present invention discloses and claims a vaccine composition comprising principally of HMGB1 from Plasmodium falciparum (Pf HMGB1) and adjuvants for protection against respective malarial parasite. Further, the present invention claims a process for preparation of a vaccine composition against malaria comprising the steps of expressing pfHMGB1 in BL21DE3 E. coli cells through transferring in presence of ampicillin while inducing with IPTG; pelleting down the expressed cells through centrifugation following suspension in buffer; sonicating; separating the aqueous extract of cell lysate by further centrifugation; purifying of the resultant by Ni-NTA column using imidazole; quantifying of protein and screening for integrity of purified protein on 12% SDS-PAGE; and finally mixing of pfHMGB1 protein in PBS with either Freund’s Complete Adjuvant, for first dose or with 75 ul of Freund’s Incomplete Adjuvant, for subsequent dose(s).

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Notices, Deadlines & Correspondence

Patent Information

Application #

Filing Date

04 October 2017

Publication Number

14/2019

Publication Type

INA

Invention Field

PHARMACEUTICALS

Status

Email

lsdavar@ca12.vsnl.net.in

Parent Application

Applicants

INSTITUTE OF LIFE SCIENCES

(An autonomous Institution under Department of Biotechnology, Government of India), Bhubaneswar-751023, India.

Inventors

1. B. Ravindran

INSTITUTE OF LIFE SCIENCES(An autonomous Institution under Department of Biotechnology, Government of India), Bhubaneswar-751023, India.

2. Arun Nagaraj

INSTITUTE OF LIFE SCIENCES(An autonomous Institution under Department of Biotechnology, Government of India), Bhubaneswar-751023, India.

3. Diwakar Singh

INSTITUTE OF LIFE SCIENCES(An autonomous Institution under Department of Biotechnology, Government of India), Bhubaneswar-751023, India.

4. Birendra Pursty

INSTITUTE OF LIFE SCIENCES(An autonomous Institution under Department of Biotechnology, Government of India), Bhubaneswar-751023, India.

5. Sagar Gaikwad

INSTITUTE OF LIFE SCIENCES(An autonomous Institution under Department of Biotechnology, Government of India), Bhubaneswar-751023, India.

6. Dileep Vasudevan

INSTITUTE OF LIFE SCIENCES(An autonomous Institution under Department of Biotechnology, Government of India), Bhubaneswar-751023, India.

Specification

DESC:Please find the attached file ,CLAIMS:Please find the attached file

Documents

Application Documents

#	Name	Date
1	201731035230-STATEMENT OF UNDERTAKING (FORM 3) [04-10-2017(online)].pdf	2017-10-04
2	201731035230-PROVISIONAL SPECIFICATION [04-10-2017(online)].pdf	2017-10-04
3	201731035230-FORM 1 [04-10-2017(online)].pdf	2017-10-04
4	201731035230-DRAWINGS [04-10-2017(online)].pdf	2017-10-04
5	201731035230-Proof of Right (MANDATORY) [14-11-2017(online)].pdf	2017-11-14
6	201731035230-FORM-26 [01-12-2017(online)].pdf	2017-12-01
7	201731035230-DRAWING [04-10-2018(online)].pdf	2018-10-04
8	201731035230-CORRESPONDENCE-OTHERS [04-10-2018(online)].pdf	2018-10-04
9	201731035230-COMPLETE SPECIFICATION [04-10-2018(online)].pdf	2018-10-04
10	201731035230-FORM 18 [21-05-2019(online)].pdf	2019-05-21
11	201731035230-NBA CORRESPONDENCE-(28-08-2019).pdf	2019-08-28
12	201731035230-FER.pdf	2021-10-18
13	201731035230-FORM 4(ii) [17-11-2021(online)].pdf	2021-11-17
14	201731035230-EDUCATIONAL INSTITUTION(S) [17-11-2021(online)].pdf	2021-11-17
15	201731035230-(21-03-2022)-NBA COMMUNICATION.pdf	2022-03-21
16	201731035230-AbandonedLetter.pdf	2024-07-11

Search Strategy

1	2021-05-2016-22-06E_20-05-2021.pdf