DESC:FIELD OF THE INVENTION
The present invention relates to process for preparation of a slow and sustained release pheromones which releases the desired pheromone substances into air for monitoring or mass trapping or mating disruption of pests), and more particularly it relates to a process for preparation of pheromone lure specific for trapping and monitoring Red Palm Weevil (Rhynchophorus ferrugineus).

BACKGROUND OF THE INVENTION

A pheromone is a chemical signalling compound naturally produced by many living organisms that send information to other organisms of the same species. Pheromones are non-toxic, they have no adverse effects on non-target organisms, if applied or spread it does not kill parasitoids or other beneficial insects. All these features make the pheromones a strong candidate for controlling insect population. However, the same has not be done at larger scale. The biggest drawbacks of using pheromones to control insect populations is the cost of the insect pheromone. Most pheromone components vaporize quickly thereby defeating the whole purpose itself. If such pheromones are not formulated in an appropriate sustain-release dispenser base it cannot deployed in field. An insect pest generally emerges over a long period of time. So, it is a requirement of sustained release of a pheromone substance from the dispenser base to make the monitoring or mass trapping or mating disruption for the entire period of emergence.

The existing insect trapping or monitoring devices can be exemplified as follows, the material includes rubber, polyethylene, polypropylene, an ethylene-vinyl acetate copolymer containing 90% by weight or greater of ethylene repeating units, and polyvinyl chloride. The polyolefin-based plastics are also widely used since they are inexpensive. Each of these presently known and used devices and methods have certain advantages and drawbacks. The lures used in the above-mentioned devises are neither biodegradable nor release duration of the pheromone covers the entire crop cycle while being costly for mass use.

It is required for effective pest management to develop a control technology with pheromone to sustainably release a sufficient amount of pheromone from an attraction source to attract insect pests with respect to the monitoring or mass trapping or mating disruption and to maintain a sufficient amount of pheromone to cause mating disruption in an entire region to be controlled with respect to the mating disruption. Ideally, the release rate of the pheromone should be relatively constant and remain effective during the whole flight period of the pest.

Therefore, there is an urgent need for a process for preparing a slow and sustain release dispersion medium for pheromones which are eco-friendly and biodegradable.

SUMMARY OF THE INVENTION

The present disclosure provides for a process preparing a bio-degradable angstrom voids polymer (AVP) matrix gel and its formulation for a slow and sustained release of pheromone 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure.

In an embodiment of the present invention, the pheromone lure comprises pheromone 4-methyl-5-nonanol, 4-methyl-5-nonanone and a kairolure impregnated in angstrom voids polymer (AVP) matrix gel as sustained release dispensing medium. In the present invention, AVP matrix gel helps the pheromone in retaining their stability and enables the active ingredients to release at a continuous and slow release rate for a prolonged period of time. However, it is also necessary to instant release high amount of pheromone substance in order to reach the pests which are far away. On the other hand, it is also necessary to achieve extended release of a pheromone substance from the dispenser to make monitoring or mass trapping or the mating disruption for the entire period of pest emergence to Red Palm Weevil (Rhynchophorus Ferrugineus).

In an embodiment, the present invention provides for a process for preparation of bio-degradable angstrom voids polymer (AVP) matrix gel for a slow and sustained release of pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure. The process, include steps of:

(a) mixing pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure and Zeolite in a container to get both the pheromones and Kairolure incorporated in angstrom voids polymer (AVP) matrix gel, where both the pheromones being allowed to be completely absorbed by Zeolite;

(b) preparing pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure gel matrix by adding one or more gelling agents to water while mixture being continuously stirred, where the one or more gelling agents being added lot by lot and allowed to swell,
adding to the mixture obtained in previous step at least one of one or more preserving agents, one or more moisture retaining agents, one or more anti-microbial agents, one or more anti-oxidizing agents, and one or more UV-blockers, while continuously stirring,
adding one or more surfactants and one or more colouring agents to the mixture obtained in the previous step, and
adding the pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure dissolved in ethanol, to the mixture obtained in the previous step while being continuously stirred, thereby obtaining the pheromones gel matrix;

(c) mixing the both pheromones, 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure, incorporated in zeolites, angstrom voids polymer (AVP) with the pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure gel matrix thereby obtaining the bio-degradable angstrom voids polymer (AVP) matrix gel for a slow and sustained release of the pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure.; and

(d) adding one or more organic pH modifiers to the bio-degradable angstrom voids polymer (AVP) matrix gel to adjust pH to 6.0 - 7.5., preferably in pH range 6.5-7.0.

In another embodiment, the present invention provides for a bio-degradable angstrom voids polymer (AVP) matrix gel for a slow and sustained release of pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure impregnated on Zeolite angstrom voids polymer (AVP), mixed with pheromones gel matrix comprising pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure, one or more gelling agents, one or more preserving agents, one or more moisture retaining agents, one or more anti-microbial agents, one or more anti-oxidizing agents, and one or more UV-blockers, organic pH modifiers and one or more surfactants. The pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure impregnated in bio-degradable polymer matrix gives instant release of the pheromones as well as pheromones incorporated in angstrom voids spaces of zeolite gives slow and sustained release of the pheromones.

DETAILED DESCRIPTION OF THE PRESENT INVENTION

The present invention provides for a process for preparation of pheromone lure based on bio-degradable angstrom voids polymer (AVP) matrix gel, one or more pheromones and a kairolure to be used for trapping and/or monitoring Red Palm Weevil (Rhynchophorus ferrugineus). The pheromone lure is designed for a slow and sustained release of the one or more pheromones suitable for attracting the Red Palm Weevil (RPW).

RPW larvae can excavate holes in the trunk of a palm trees up to a metre long, thereby weakening and eventually killing the host plant. As a result, the weevil is considered a major pest in palm plantations, including the coconut palm, date palm and oil palm. The present invention provides for an efficient and eco-friendly solution for pest managing of the RPW.

In an embodiment of the present invention utilises zeolites for making the AVP matrix gel. The Zeolites are crystalline solids structures mainly consist of Si, Al, O, and metals including Ti, Sn, Zn, and so on that form a framework with cavities and channels inside where cations, water and/or small molecules may reside. The Zeolites are also called as molecular sieves. Many of zeolites occur naturally as minerals and are extensively mined in many parts of the world finding applications in industry and medicine. However, most of zeolites have been made synthetically some of them made for commercial use while others created by scientists to study their chemistry.

In an embodiment, the present invention provides for a process for preparation of the pheromone lure using the bio-degradable angstrom voids polymer (AVP) matrix gel, pheromone 4-methyl-5-nonanol and 4-methyl-5-nonanone and the kairolure. The process, comprises steps of:

(a) mixing the pheromone 4-methyl-5-nonanol, the pheromone 4-methyl-5-nonanone, the kairolure and Zeolite in a container to get the pheromones incorporated in the angstrom voids polymer (AVP) of zeolite, wherein both the pheromones 4-methyl-5-nonanol, and pheromone 4-methyl-5-nonanone being allowed to be completely absorbed by the Zeolite;

(b) preparing one or more pheromones’ gel matrix by
adding one or more gelling agents to water to obtain a first mixture, while being continuously stirred, where the one or more gelling agents being added lot by lot to the water and allowed to swell;
adding to the first mixture one or more preserving agents, one or more moisture retaining agents, one or more anti-microbial agents, one or more anti-oxidizing agents, one or more pH stabilizers, and one or more UV-blockers, and one or more surfactants, and one or more preservatives or combination thereof to obtain a second mixture, while being continuously stirred;
adding one or more surfactants and one or more colouring agents to the second mixture to obtain a third mixture; and
adding the pheromones 4-methyl-5-nonanol and 4-methyl-5-nonanone, the kairolure dissolved in ethanol to the third mixture, while being continuously stirred, to obtain the pheromone gel matrix;

(c) mixing (i) the pheromone 4-methyl-5-nonanolthe 4-methyl-5-nonanone and the kairolure incorporated in the angstrom voids polymer (AVP) of zeolite with (ii) the one or more pheromones’ gel matrix, thereby obtaining the bio-degradable angstrom voids polymer (AVP) matrix gel as the pheromone lure for a slow and sustained release pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure; and

(d) adding one or more organic pH modifiers to the bio-degradable angstrom voids polymer (AVP) matrix to adjust pH to 6.0 - 7.5 to obtain the pheromone lure. The pH range may be preferably adjusted to 6.5 - 7.0.

The kairolure used herein comprises of Vanillin and one or more surfactants. The one or more surfactants are selected from a group consisting of Triethanolamine, Triton x 100, Triethanolamine, Propylene glycol, Triton-X, Diethanolamine, sodium dodecyl sulphate, Polyethylene glycol, Alkyl polyglycoside, Cetomacrogol 1000, Cetostearyl alcohol, Cetyl alcohol, Cocamide DEA, Cocamide MEA, Decyl glucoside, Decyl polyglucose, Disodium cocoamphodiacetate, Glycerol monostearate, IGEPAL CA-630, Isoceteth-20, Lauryl glucoside, Maltosides, Monolaurin, Mycosubtilin, Narrow-range ethoxylate, Nonidet P- 40, Nonoxynol-9, Nonoxynols, NP-40, Octaethylene glycol monododecyl ether, N-Octyl beta-D-thioglucopyranoside, Octyl glucoside, Oleyl alcohol, PEG-10 sunflower glycerides, Pentaethylene glycol, monododecyl ether, Polidocanol, Poloxamer, Poloxamer 407, Polyethoxylated tallow amine, Polyglycerol polyricinoleate, Polysorbate, Polysorbate 20, Polysorbate 80, Sorbitan, Sorbitan monolaurate, Sorbitan, monostearate, Sorbitan tristearate, Stearyl alcohol, Surfactin and Tween 80.

To store the pheromone lure (the bio-degradable AVP matrix) for long term and to increase its effectiveness, it has to be protected from physico-chemical factors, the abiotic and biotic factors like UV light, moisture, high temperature and micro-organisms.

The anti-oxidising agents are selected from a group consisting of Butylated Hydroxy Toluene (BHT), Butylated hydroxyl anisole (BHA), vitamin E, vitamin C, 2,6-di-tert-butyl-p-cresol, Tertiary butyl hydroxyquinone, Lecithin soya 30%, 2,5-di-tert-butylhydroquinone. More preferably BHT and BHA. The stoichiometric ratio of the antioxidant agents in the gel matrix were added in the ratio about 0.1 to 3%, more preferably 0.5 to 1.5%.

The moisture retaining agents are selected from a group consisting of PEG (polyethylene glycol), PPG (polypropylene glycol), Glycerine. More preferably PEG and Glycerine. The stoichiometric ratio of the moisture retaining agents in the gel matrix were added in the ratio about 2 to 10%, more preferably 4 to 6%.

The anti-microbial agents are selected from a group consisting of sodium benzoate, sodium sorbate, potassium sorbate, propylene oxide, Benzalkonium chloride, Sorbic acid, Boric acid, Thiomersal, methyl-4-hydroxy benzoate. More preferably sodium benzoate, sodium sorbate. The stoichiometric ratio of the anti-microbial agents in the gel matrix were added in the ratio about 0.1 to 3%, more preferably 0.5 to 1.5%.
The one or more pH stabilizers added in the in the gel matrix in predefined stoichiometric ratio of 0.5 to 1.0%, more preferably 0.1 to 0.25%.

The UV-blockers are selected from a group consisting of Para-Amino benzoic acid, Zinc oxide, Benzophenone, Ethyl cinnamate. The stoichiometric ratio of the UV-blockers in the gel matrix were added in the ratio about 0.01 to 0.5%, more preferably 0.05 to 0.2%.

The preserving agents are selected from a group consisting of 2-chloroacetamide, Sodium sorbate, Potassium sorbate, Calcium sorbate, Benzoic acid, Sodium benzoate, Potassium benzoate, Calcium benzoate, Propylparaben or Propyl-p-hydroxy-benzoate, Methylparaben or Methyl-p-hydroxy-benzoate, Sulphur dioxide, Sodium sulphite, Sodium bisulphate, Sodium meta bisulphite, Potassium meta bisulphite, Potassium sulphite, Potassium bisulphate, Nisin, Natamycin or Pimaricin, Dimethyl dicarbonate, Potassium nitrite, Sodium nitrite, Sodium nitrate, Potassium nitrate, Propionic acid, Sodium propionate, Calcium propionate, Potassium propionate, Calcium disodium ethylenediaminetetraacetate or calcium disodium EDTA, Lysozyme. More preferably 2-chloroacetamide and benzyl alcohol. The stoichiometric ratio of the preserving agents in the gel matrix were added in the ratio about 0.1 to 3%, more preferably 0.5 to 1.5%.

One or more surfactants such as Triethanolamine, Triton x 100, Triethanol amine, Propylene glycone, Criton-X, Diethanol amine, sodium dodecyl sulphate, Poly ethylene glycol, Alkyl polyglycoside, Cetomacrogol 1000, Cetostearyl alcohol, Cetyl alcohol, Cocamide DEA, Cocamide MEA, Decyl glucoside, Decyl polyglucose, Disodium cocoamphodiacetate, Glycerol monostearate, IGEPAL CA-630, Isoceteth-20, Lauryl glucoside, Maltosides, Monolaurin, Mycosubtilin, Narrow-range ethoxylate, Nonidet P-40, Nonoxynol-9, Nonoxynols, NP-40, Octaethylene glycol monododecyl ether, N-Octyl beta-D-thioglucopyranoside, Octyl glucoside, Oleyl alcohol, PEG-10 sunflower glycerides, Pentaethylene glycol, monododecyl ether, Polidocanol, Poloxamer, Poloxamer 407, Polyethoxylated tallow amine, Polyglycerol polyricinoleate, Polysorbate, Polysorbate 20, Polysorbate 80, Sorbitan, Sorbitan monolaurate, Sorbitan, monostearate, Sorbitan tristearate, Stearyl alcohol, Surfactin, Triton X-100, and Tween 80. One or more of the surfactants were added in small amount which raises the pH and increases the swelling capacity of gelling agent. The one or more surfactants added in the predefined stoichiometric ratio of 0.01 to 0.5%, more preferably 0.01 to 0.25%.

The gelling agents are selected from a group consisting of Carbopol 940, Gelatin, Pluronic, sodium alginate, Beta cyclodextrin, Bentonite, Methyl cellulose, Carboxy methyl cellulose, chitosan, carboxy methyl chitosan, gellan gum, carrageenan, xanthan gum, silica, mannitol dioctanoate, PVA(poly vinyl alcohol), tragacanth gum, hydroxyethyl cellulose, hydroxypropyl cellulose, Locust bean gum, Pectin, Starch, agars, magnesium aluminium silicate. Preferred grades of polyacrylic acid are those referred to as Carbopol at concentrations of 0.5-10.0%. Higher concentrations of polyacrylic acid lower the release rate of the active components. Viscosities of polyacrylic acids are generally stable between pH 6.0 - 7.0, preferably in the pH range 6.5-7.0. The viscosity of the gels prepared ranged from about 0 to about 80,000 cps.

The special bio-degradable angstrom voids polymer (AVP) matrix formulation of the pheromone lure enables it to have instant release of high amount of the pheromones as well as extended release of a pheromones. The instant release of high amount of the pheromones is necessary to reach the pests which are far away. On the other hand, the extended release of the pheromones from the dispenser to make monitoring or mass trapping or the mating disruption for the entire period of pest emergence with respect to the Red Palm Weevil.

The bio-degradable angstrom voids polymer (AVP) matrix formulation of the pheromone lure may be used in insect traps for population monitoring or trapping as a part of pest control. For any lure to be used in such insect traps requires a very specific delivery rate. A too low delivery rate may be below the threshold of perceptions while too high delivery rate may confuse or repel the insect and prevent the insects from entering the trap. However, the present bio-degradable angstrom voids polymer (AVP) matrix formulation of the pheromone lure provides an optimum delivery rate suitable to be used efficiently in the insect traps.
The process disclosed by present invention enables preparation of novel, cost effective, slow and extended release of impregnated pheromone in angstrom voids polymer (AVP) matrix. The AVP matrix with the pheromolecules serves both purposes of instant release of high amount of pheromone substance in order to reach the pests which are far away and extended release of the pheromone substance. These two features make the AVP matrix with the pheromolecules a suitable candidate to be used in insect trapping devices to enable monitoring or mass trapping or the mating disruption for the entire period of pest emergence.

In yet another embodiment of the present invention, a pheromone lure having properties to attract RPW is provided. The pheromone lure consists of pheromone impregnated in angstrom voids polymer (AVP) matrix as sustained release dispensing medium. The pheromone retains their stability and the active ingredients are allowed to release at a continuous slow release rate for a prolonged period of time. However, it is necessary to instant release of high amount of pheromone substance in order to reach the pests which are far away. On the other hand, it is necessary to require extended release of a pheromone substance from the dispenser to make monitoring or mass trapping or the mating disruption for the entire period of pest emergence. Thus, the present invention AVP matrix consists together of pheromone impregnated bio-degradable polymer which gives instant release of pheromone as well as pheromone incorporated in angstrom voids polymer matrix extended release of the pheromone. In addition, the pheromone formulation is protected from the degradation of UV light, moisture, and high temperature by addition of antioxidants, pH stabilizers, moisture retainers, anti-microbial agent, surfactants and UV blockers.

EXAMPLES
The present invention is explained further in the following specific examples which are only by way of illustration and are not to be construed as limiting the scope of the invention.
Example 1: Procedure for preparation of 1000 grams of gel

The procedure requires a wide mouthed container selected based on the amount of gel to be prepared. The container should be provided with a stirrer for the uniform mixing of the all the ingredients. For the 1000 gms of the gel preparation one can use about 2.0 L volume containers. 5.0-100.0 grams of Carbopol (0.5 to 10%) was added to the solution and mixed slowly by stirring with the use of a mechanical stirrer. The Carbopol was allowed to swell. Added other additives at the concentration of 0.1-5% by weight. Added pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure, dissolved in suitable eco-friendly organic solvent. Finally added zeolites (pore size - 400 Picometers) impregnated with 10.0 - 50.0% bioactive agent and mixed well for uniform distribution. The final formulation mixture was adjusted to pH 6.5 to 7.5 by the addition of organic pH modifiers.

The pheromone lure consists of Zeolite biodegradable polymer matrix gel, wherein which the pheromone present in a zeolite bio-degradable polymer matrix in an amount varying from about 0.5 % to 5.0 % by weight, more preferably 1%. The angstrom voids polymer (AVP) matrix and its formulations act as a sustain release dispersion medium for pheromones. Further, the pheromone lure is bio-degradable, non-polluting, cost effective, eco-friendly and efficacious.

The present invention may boost the study of RPW by trapping and monitoring, and thereby will enable finding of better pest management.

The use of pheromone lure may result in capturing of the RPW which is surely expected to improve yield of palm plantations, including the coconut palm, date palm and oil palm.

While the invention has been described in detail with reference to preferred embodiments thereof, it will be apparent to one skilled in the art that various changes can be made and equivalents employed, without departing from the scope of the invention.
,CLAIMS:WE CLAIM:

1. A process for preparation of pheromone lure for attracting red palm weevil, comprising:

mixing a pheromone 4-methyl-5-nonanol, a pheromone 4-methyl-5-nonanone, a kairolure and Zeolite so as to allow absorption of the pheromone 4-methyl-5-nonanol, the pheromone 4-methyl-5-nonanone and the kairolure in angstrom voids (AV) of the zeolite and thereby forming angstrom voids polymer (AVP);

preparing pheromone gel matrix, wherein the preparing comprises

adding one or more gelling agents to water, while being continuously stirred, where the one or more gelling agents being added lot by lot to the water and allowed to swell to obtain a first mixture,

adding at least one of one or more preserving agents, one or more moisture retaining agents, one or more anti-microbial agents, one or more anti-oxidizing agents, one or more pH stabilizers, and one or more UV-blockers, and one or more preservatives to the first mixture, while being continuously stirred, to obtain a second mixture;

adding one or more surfactants and one or more colouring agents to the second mixture, while being continuously stirred, to obtain a third mixture; and

adding the pheromones 4-methyl-5-nonanol and 4-methyl-5-nonanone, the kairolure dissolved in ethanol to the third mixture, while being continuously stirred, to obtain the pheromone gel matrix;

mixing the angstrom voids polymer (AVP) of zeolite with the pheromone gel matrix, to obtain an angstrom voids polymer (AVP) matrix gel; and

adding one or more organic pH modifiers to the angstrom voids polymer (AVP) matrix to adjust pH to 6.0 - 7.5 to obtain the pheromone lure for attracting red palm weevil (RPW),

wherein the pheromone lure enables slow and sustained release pheromones 4-methyl-5-nonanol, 4-methyl-5-nonanone and kairolure.

2. The process as claimed in claim 1, wherein the kairolure comprises vanillin and one or more surfactants, wherein the one or more surfactants are selected from a group consisting of Triethanolamine, Triton x 100, Triethanolamine, Propylene glycol, Triton-X, Diethanolamine, sodium dodecyl sulphate, Polyethylene glycol, Alkyl polyglycoside, Cetomacrogol 1000, Cetostearyl alcohol, Cetyl alcohol, Cocamide DEA, Cocamide MEA, Decyl glucoside, Decyl polyglucose, Disodium cocoamphodiacetate, Glycerol monostearate, IGEPAL CA-630, Isoceteth-20, Lauryl glucoside, Maltosides, Monolaurin, Mycosubtilin, Narrow-range ethoxylate, Nonidet P- 40, Nonoxynol-9, Nonoxynols, NP-40, Octaethylene glycol monododecyl ether, N-Octyl beta-D-thioglucopyranoside, Octyl glucoside, Oleyl alcohol, PEG-10 sunflower glycerides, Pentaethylene glycol, monododecyl ether, Polidocanol, Poloxamer, Poloxamer 407, Polyethoxylated tallow amine, Polyglycerol polyricinoleate, Polysorbate, Polysorbate 20, Polysorbate 80, Sorbitan, Sorbitan monolaurate, Sorbitan, monostearate, Sorbitan tristearate, Stearyl alcohol, Surfactin and Tween 80.

3. The process as claimed in claim 1, wherein the pH of the pheromone lure preferably adjusted to have in pH range 6.5-7.0.

4. The process as claimed in claim 1, wherein at least one of the one or more preserving agents, the one or more moisture retaining agents, the one or more anti-microbial agents, the one or more anti-oxidizing agents, the one or more pH stabilizers, the one or more UV-blockers, one or more surfactants, and one or more preservatives being added to the first mixture in a predefined stoichiometric ratio, wherein

the one or more anti-oxidizing agents added in the predefined stoichiometric ratio of 0.1 to 3%, more preferably 0.5 to 1.5%;
one or more pH stabilizers added in the predefined stoichiometric ratio of 0.5 to 1.0%, more preferably 0.1 to 0.25%;
one or more moisture retainers are added in predefined stoichiometric ratio of 2 to 10%, more preferably 4 to 6%;
one or more anti-microbial agents are added in predefined stoichiometric ratio of 0.1 to 3%, more preferably 0.5 to 1.5%;
one or more surfactants added in the predefined stoichiometric ratio of 0.01 to 0.5%, more preferably 0.01 to 0.25%;
one or more preservatives in the predefined stoichiometric ratio of 0.1 to 3%, more preferably 0.5 to 1.5%; and
one or more UV blockers in the predefined stoichiometric ratio of 0.01 to 0.5%, more preferably 0.05 to 0.2%.

5. The process as claimed in claim 1, wherein the one or more anti-oxidizing agents being selected from a group consisting of Butylated Hydroxy Toluene (BHT), Butylated hydroxyl anisole (BHA), vitamin E, vitamin C, 2,6-di-tert-butyl-p-cresol, Tertiary butyl hydroxyquinone, Lecithin soya 30%, 2,5-di-tert-butylhydroquinone. More preferably BHT and BHA.

6. The process as claimed in claim 1, wherein the moisture retaining agents are selected from a group consisting of PEG (polyethylene glycol), PPG (polypropylene glycol), Glycerine.

7. The process as claimed in claim 1, wherein the anti-microbial agents are selected from a group consisting of sodium benzoate, sodium sorbate, potassium sorbate, propylene oxide, Benzalkonium chloride, Sorbic acid, Boric acid, Thiomersal, methyl-4-hydroxy benzoate.

8. The process as claimed in claim 1, wherein the UV-blockers are selected from a group consisting of Para-Amino benzoic acid, Zinc oxide, Benzophenone, and Ethyl cinnamate.

9. The process as claimed in claim 1, wherein the preserving agents are selected from a group consisting of 2-chloroacetamide, Sodium sorbate, Potassium sorbate, Calcium sorbate, Benzoic acid, Sodium benzoate, Potassium benzoate, Calcium benzoate, Propylparaben or Propyl-p-hydroxy-benzoate, Methylparaben or Methyl-p-hydroxy-benzoate, Sulphur dioxide, Sodium sulphite, Sodium bisulphate, Sodium metabisulphite, Potassium metabisulphite, Potassium sulphite, Potassium bisulphate, Nisin, Natamycin or Pimaricin, Dimethyl dicarbonate, Potassium nitrite, Sodium nitrite, Sodium nitrate, Potassium nitrate, Propionic acid, Sodium propionate, Calcium propionate, Potassium propionate, Calcium disodium ethylenediaminetetraacetate or calcium disodium EDTA, Lysozyme.

10. The process as claimed in claim 1, wherein the surfactants are selected from a group consisting of Triethanolamine, Triton x 100, Tri ethanol amine, Propylene glycone, Criton-X, Di ethanol amine, sodium dodecyl sulphate, Poly ethylene glycol, Alkyl polyglycoside, Cetomacrogol 1000, Cetostearyl alcohol, Cetyl alcohol, Cocamide DEA, Cocamide MEA, Decyl glucoside, Decyl polyglucose, Disodium cocoamphodiacetate, Glycerol monostearate, IGEPAL CA-630, Isoceteth-20, Lauryl glucoside, Maltosides, Monolaurin, Mycosubtilin, Narrow-range ethoxylate, Nonidet P-40, Nonoxynol-9, Nonoxynols, NP-40, Octaethylene glycol monododecyl ether, N-Octyl beta-D-thioglucopyranoside, Octyl glucoside, Oleyl alcohol, PEG-10 sunflower glycerides, Pentaethylene glycol, monododecyl ether, Polidocanol, Poloxamer, Poloxamer 407, Polyethoxylated tallow amine, Polyglycerol polyricinoleate, Polysorbate, Polysorbate 20, Polysorbate 80, Sorbitan, Sorbitan monolaurate, Sorbitan, monostearate, Sorbitan tristearate, Stearyl alcohol, Surfactin, Triton X-100, and Tween 80.