FORM 2
THE PATENTS ACT, 1970
(39 of 1970)
&
The Patent Rules, 2003
COMPLETE SPECIFICATION
(See section 10 and rule 13)
TITLE OF THE INVENTION
“A PROCESS FOR PREPARATION OF
TRIAMINOPYRIMIDINE COMPOUND AND
INTERMEDIATES THEREOF”
We, ZYDUS LIFESCIENCES LIMITED, an Indian company incorporated under the Companies Act, 1956, of Zydus Corporate Park, Scheme No. 63, Survey No. 536, Plot No. 103, Khoraj(Gandhinagar), Nr. Vaishnodevi Circle, Sarkhej – Gandhinagar Highway, Ahmedabad 382481, Gujarat, India,
The following specification particularly describes the invention and the manner in which it is to be performed:

FIELD OF THE INVENTION
The present invention relates to a process for preparation of triaminopyrimidine
compound and intermediates thereof. In particular, the present invention relates to a
5 process for preparing pure triaminopyrimidine compound. The present invention also
relates to a pharmaceutical composition comprising pure triaminopyrimidine compound,
useful for preventing or treating malaria.
BACKGROUND OF THE INVENTION
10 The following discussion of the prior art is intended to present the invention in an
appropriate technical context and allow its significance to be properly appreciated. Unless clearly indicated to the contrary, however, reference to any prior art in this specification should be construed as an admission that such art is widely known or forms part of common general knowledge in the field.
15
Malaria is caused by protozoan parasites of the genus Plasmodium that infect and destroy red blood cells, leading to fever, severe anemia, cerebral malaria and, if untreated, death.
20 International (PCT) Publication No. WO 2015/165660 (the WO '660) discloses
triaminopyrimidine compounds, intermediates, pharmaceutical compositions and methods for use for preventing or treating malaria. The WO '660 discloses a process for preparation of 4-cyclopropyl-5-fluoro-6-methylpyridin-2-amine (compound 5) as depicted in scheme-1. 25
CH3 COOH CH3 NH CH3 CH3
N^SfF A N^VF Ph"%h Ph N-^VF N^VF
4a 3a 2a 5
Scheme 1
WO '660 discloses a process for preparation of triaminopyrimidine compounds as depicted in scheme-2.

CH3 Q^3
ONH ^ lAo V*
10 9 8 H 7
6
CH, PH' fHs
2b F 3
ffi,
N NH
1 F
Scheme 2
WO '660 discloses the preparation of compounds 8 and 4 by using microwave 5 technique using Biotage microwave vial. WO '660 in example-13, discloses the isolation of compound 1 by concentration of reaction mixture to obtain crude product, which was purified through reverse phase HPLC GILSON instrument to obtain pure solid compound 1 in 40.8% yield, without providing the purity of the solid compound 1. The process disclosed in WO '660 is not industrially advantageous as it requires microwave conditions 10 as well as chromatographic purification and provides compound 1 with lower yields. The compound 1 prepared may not be suitable for pharmaceutical preparations based on various regulatory requirements.

Polymorphism, the occurrence of different crystalline forms, is a property of some molecules. A single molecule can exist in different crystalline forms having distinct physical properties like melting point, thermal behaviors (e.g. measured by thermogravimetric analysis - TGA, or different scanning calorimetry - DSC, Powder x-ray 5 diffraction pattern - PXRD, infrared absorption - IR). One or more these techniques may be used to distinguish different polymorphic forms of a compound.
Different salts and solid states (e.g. solvates, hydrates) of an active pharmaceutical ingredient may possess different physio-chemical properties. Such variation in the
10 properties of different salts and solid states forms may provide a basis for improving formulation, for example, by facilitating better processing or handling characteristics, changing the dissolution profile in a favorable direction, or improving stability (both chemical and polymorph) and shelf-life. These variations in the properties of different salts and solid states forms may offer improvements to the final dosage form for example, to
15 improve bioavailability. Different salts and solid state forms of an active pharmaceutical ingredient may also give rise to a variety of polymorphs or crystalline forms or amorphous form, which may in turn provide additional opportunities to assess variations in the properties and characteristics of an active pharmaceutical ingredient.
20 In view of the above, the present invention provides a process for the preparation of
triaminopyrimidine compound 1 or pharmaceutically acceptable salts thereof or hydrates or solvates or polymorphs or optically active forms thereof, which is industrially scalable, environment friendly and efficient so as to obtain compounds of the invention in higher yields and purity.
25
The process for the preparation of triaminopyrimidine compound 1 or intermediates thereof of the present invention, takes the advantage by using appropriate solvent systems and isolation techniques as well as purification techniques, thereby to overcome problems of lower yields, chromatography purifications and microwave
30 reactions of the prior art.
SUMMARY OF THE INVENTION

The present invention provides solid state forms of triaminopyrimidine compound
1,
CH3
CH3 f^N NH
AN
F 1
or pharmaceutically acceptable salts thereof, or hydrates, or solvates, or polymorphs, or
5 optically active forms thereof, in solid state forms.
The present invention provides solid state forms of triaminopyrimidine compound 1 or pharmaceutically acceptable salts thereof and use in the preparation of pharmaceutical compositions and/or formulations for use in medicine, preferably for the treatment of 10 malaria.
In one general aspect, the present invention provides pharmaceutical compositions and/or pharmaceutical formulations comprising any one or a combination of the solid forms of triaminopyrimidine compound 1 or pharmaceutically acceptable salts thereof. 15
In another general aspect, the present invention provides different crystalline forms of triaminopyrimidine compound 1 and intermediates thereof.
In another general aspect, there is provided substantially pure triaminopyrimidine 20 compound 1 having a purity of about 98% or more by weight, of about 99% or more by weigh, of about 99.5% or more by weight, of about 99.8% or more by weight, of about 99.9% or more by weight, by area percentage of HPLC.
In another general aspect, there is provided substantially pure compound 5 or 25 hydrate thereof having a purity of about 99 % or more by weight, of about 99.5% or more by weight, of about 99.8% or more by weight, measured by area percentage by HPLC.

In another general aspect, there is provided crystalline compound 5. In general, the crystalline compound 5 is a hydrate. In particular, the crystalline compound 5 is a monohydrate. 5
In another general aspect, there is provided a process for the preparation of compound 5 or a hydrate thereof,
CH3
HO NH2 ^^ >T7 5
the process comprising:
10 (a) reacting compound 4a with cyclopropane carboxylic acid in one or more solvents to
obtain a compound 3a;
CH3 CH3
4a 3a
(b) reacting the compound 3a with diphenylmethanimine in one or more solvents to obtain
compound 2a;
CH3
Ph N*Sr*F
15 2a
(c) reacting the compound 2a with hydrochloric acid in one or more solvents to obtain the
compound 5; and
CH3
HONrif^^^-7 5
(d) obtaining the compound 5 or a hydrate thereof by crystallizing in one or more solvents.
20

In another general aspect, there is provided a process for the preparation of compound 6,
H3C ,CH3
NH2 6
the process comprising:
5 (a) halogenating 2-butenenitrile to obtain a compound 2,3-dihalobutanenitrile; and
(b) reacting the compound 2,3-dihalobutanenitrile with methyl hydrazine or salts thereof in
the presence of a base to obtain compound 6.
In another general aspect, there is provided a process for the preparation of 10 triaminopyrimidine compound 1, the process comprising:
(a) reacting compound 10 with compound 9 in one or more solvents to obtain compound
8;
CH3
10 9 8 H
(b) reacting the compound 8 with a chlorinating agent in one or more solvents to obtain
15 compound 7;
CH3
Boc^ ci
7Vc,
(c) reacting the compound 7 with compound 6 in one or more solvents in the presence of a
base to obtain compound 4;

CH3
H3C ,CH3 CH3 p4
VN Boc A A,N-CH3
NH2 T "J
4
(d) reacting the compound 4 with compound 5 or a hydrate thereof in one or more solvents
in the presence of a base to obtain compound 3;
CH3
CH3 r=BOC. J\ ^ 'N~~CH3
N^ HN N
CH3 ^NY^N
FYS lNANH
F 3
5 (e) reacting the compound 3 or solution thereof with an acid in one or more solvents to
obtain compound 2 free base;
CH3
X X ^-CH3
HN ^ HN N
N NH
\7T^CH3
F 2
(f) reacting the compound 2 free base with a methylating agent in one or more solvents to obtain the compound 1; and 10 (g) optionally, crystallizing the compound 1 in one or more solvents.
In another general aspect, there is provided a free base compound 2, which is an intermediate for the preparation of triaminopyrimidine compound 1,

CH3
CH3 r=HN-^^I HN N N NH
V-V-CH3
2
In another general aspect, there is provided a pharmaceutical composition comprising crystalline triaminopyrimidine compound 1, and pharmaceutical^ acceptable carrier, diluents and excipients. 5
In another general aspect, there is provided a pharmaceutical composition comprising substantially pure triaminopyrimidine compound 1, and one or more of pharmaceutically acceptable carrier, diluents and excipients.
10 BRIEF DESCRIPTION OF DRAWINGS
FIG.l: X-ray powder diffraction pattern of crystalline compound 1 (Ex-16) FIG.2: Differential Scanning Calorimetry of crystalline compound 1 (Ex-16) FIG.3: X-ray powder diffraction pattern of crystalline compound 1 (Ex-17) FIG.4: Differential Scanning Calorimetry of crystalline compound 1 (Ex-17) 15 FIG.5: X-ray powder diffraction pattern of compound 5 (Ex-6) FIG.6: Differential Scanning Calorimetry of compound 5 (Ex-6) FIG.7: Thermogravimetric analysis of compound 5 (Ex-6)
DETAILED DESCRIPTION OF THE INVENTION
20 The above and other objects of the present invention are achieved by the process of
the present invention, which leads a process for the preparation of pure triaminopyrimidine compound 1 in crystalline form and hydrate form of compound 5 in one or more solvents.

PH3
CH3 r==HoC X X N-CH3
N NH
^N CH3
F HO NHa-^^^ry
1 5 V
Optionally, the solution, prior to any solids formation, can be filtered to remove
any undissolved solids or solid impurities prior to removal of the solvent. Any filtration
system and filtration techniques known in the art can be used.
5
All ranges recited herein include the endpoints, including those that recite a range
"between" two values. Term "substantially" is to be construed as modifying a term or
value such that it is not an absolute. This includes, at very least, the degree of expected
experimental error, technique error and instrument error for a given technique used to
10 measure a value.
The term "substantially pure" means a compound having a purity of at least about
98% or more, by area percentage of HPLC. In particular, the compound is having a purity
of at least about 99% or more, more particularly, a purity of at least about 99.5% or more,
15 further more particularly, a purity of at least about 99.8% or more, most particularly, a
purity of at least about 99.9% or more by area percentage of HPLC.
The term "substantially free" means triaminopyrimidine compound 1 having one or more each of compound 11, compound 12, compound 13, compound 14, compound 15 or 20 other such compounds, of about 1% or less by area percentage of HPLC. In particular, the triaminopyrimidine compound 1 is having one or more each of these compounds, of about 0.5% or less, of about 0.25% or less, of about 0.20% or less, of about 0.15% or less, of about 0.10% or less, of about 0.05% or less, or of about not in detectable amount by an area percentage of HPLC.
9S

As used herein the terms, "treating", "reacting", or "condensing" have meanings as widely used by general prior art in the field of invention and can be easily understood by those skilled in the art.
5 As used herein the terms, "obtaining", or "getting" may include filtration, filtration
under vacuum, centrifugation, and decantation for isolation of the product. The product obtained may be further or additionally dried to achieve the desired moisture values. For example, the product may be dried in a tray drier, dried under vacuum and/or in a Fluid Bed Drier. The product may be preceded for further reaction with or without isolation and 10 with or without drying in case of the product was isolated.
As used herein, the term "solution" or "reaction mixture" does not limit to a clear solution only and includes any hazy or opaque mass obtained.
15 The term "composition" used herein means a physical mixture of two or more
components.
The term "pharmaceutically acceptable" means that which is useful in preparing a pharmaceutical composition that is generally non-toxic and is not biologically undesirable, 20 and includes that which is acceptable for veterinary use and/or human pharmaceutical use.
The term "pharmaceutical composition" is intended to encompass a drug product including the active ingredient(s), pharmaceutically acceptable excipients that make up the carrier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients. Accordingly, the 25 pharmaceutical compositions encompass any composition made by admixing the active ingredient, active ingredient dispersion or composite, additional active ingredient(s), and pharmaceutically acceptable excipients.
The term "pharmaceutically acceptable salts" as used herein includes but not
30 limited to acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate,
bromide, calcium edetate, camsylate, carbonate, chloride, clavulanate, citrate,
dihydrochloride, edetate, edislyate, estolate, esylate, ethylsuccinate, fumarate,gluceptate,
gluconate, glutamate, glycolylarsanilate, hexylresorcinate, hydrabamine, hydrobromide,

hydrochloride, iodide, isothionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylsulfate, mucate, napsylate, nitrate, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, phospate/diphosphate, polygalacturonate,salicylate, stearate, subacetate, succinate, tannate, tartrate,teoclate, tosylate, triethiodode, and 5 valerate.
As used herein the term "crystallizing" refers to a process comprising: heating a mixture of a starting material and a solvent to a temperature of between about 10 °C below and above the reflux temperature of the solvent to obtain a solution, and cooling the 10 solution to a temperature of about 0 °C to about 35 °C.
The terms used throughout the description is defined herein below.
"BINAP" refers to 2,2'-bis-(diphenylphosphino)-l,l'-binaphthyl
"TEA" refers to Triethylamine 15 "DIPEA" refers to N,N-Diisopropylethylamine
"STB" refers to Sodium-tert-butoxide
"PTB" refers to Potassium-tert-butoxide
"Xantphos" refers to 4,5-Bis-(diphenylphosphino)-9,9-dimethylxanthene
"XPhos" refers to 2-Dicyclohexylphosphino-2', 4', 6'-triisopropylbiphenyl 20 "DBU" refers to l,8-Diazabicyclo-(5.4.0)-undec-7-ene
"DABCO" refers to l,4-Diazabicyclo-[2.2.0]-octane
In one general aspect, there is provided a triaminopyrimidine compound 1,
CH3
CH3 fM
H^N^ HNAN'N~CH3 N NH
1
25 or pharmaceutical^ acceptable salts thereof, or hydrates, or solvates, or polymorphs, or optically active forms thereof, in solid state forms.

In another general aspect, the present invention disclosure provides different crystalline forms of triaminopyrimidine compound 1, or intermediates thereof.
5 In general, the present invention provides crystalline forms of triaminopyrimidine
compound 1. The crystalline forms of triaminopyrimidine compound 1 are represented hereinafter as "Form I" and "Form II".
In general, the present invention provides a crystalline Form I of 10 triaminopyrimidine compound 1. In general, the crystalline Form I triaminopyrimidine compound 1 is characterized by powder x-ray diffraction pattern having peaks expressed in degrees 29 at 6.3° and 9.8° ± 0.2 20.
In general, the crystalline Form I of the present invention is further characterized 15 by powder x-ray diffraction pattern having peaks expressed in degrees 29 at 6.3°, 7.8, 9.8°, 15.4°, and 20.0°± 0.2 29; a differential scanning calorimetry analysis having onset at about 179 °C± 5 °C and endothermic peak at about 181 °C± 5 °C.
In general, the present invention provides crystalline Form I of triaminopyrimidine 20 compound 1 further characterized by powder x-ray diffraction pattern substantially same as depicted in FIG.l and differential scanning calorimetry substantially same as depicted in FIG.2.
In general, the present invention provides crystalline Form II of triaminopyrimidine 25 compound 1. In general, the crystalline Form II of the present invention is characterized by powder x-ray diffraction pattern having peaks expressed in degrees 29 at 13.8°, 21.9°, and 23.7°±0.2 29.
In general, the crystalline Form II of the present invention is further characterized
30 by powder x-ray diffraction pattern having peaks expressed in degrees 29 at 8.3 ° , 12.1 ° ,
13.8°, 21.9°, and 23.7°±0.2 29; a differential scanning calorimetry analysis having atleast
one onset at about 161 °C± 5 °C and endothermic peak at about 163 °C ± 5 °C; a
differential scanning calorimetry having one onset at about 161 °C ± 5 °C and second onset

at about 179 °C ± 5 °C; a differential scanning calorimetry having one endothermic peak at about 163 °C ± 5 °C and second endothermic peak at about 181 °C ± 5 °C.
In general, the present invention provides crystalline Form II of triaminopyrimidine 5 compound 1 further characterized by powder x-ray diffraction pattern substantially as same as depicted in FIG.3 and differential scanning calorimetry substantially as same as depicted in FIG.4.
In another general aspect, there is provided compound 5, or a hydrate thereof,
CH3
HO NH/^^'^-7
10 5
In general, the compound 5 is monohydrate, having water loss of about 8% or more substantially as shown by thermogravimetric analysis.
In another general aspect, there is provided crystalline compound 5. In general, the 15 crystalline compound 5 is a hydrate. In particular, the crystalline compound 5 is monohydrate.
In general, the crystalline compound 5 is further characterized by powder x-ray powder diffraction pattern having peaks expressed in degrees 20 at 10.5°, 12.7°, and 25.1°± 20 0.2 20.
In general, the crystalline compound 5 is further characterized by powder x-ray
diffraction pattern having peaks expressed in degrees 20 at 10.5 °, 12.7 °, 25.1°, and 25.7°±
0.2 20; a differential scanning calorimetry analysis having one onset at about 106 °C± 5 °C
25 and endothermic peak at about 108 °C± 5 °C; a thermogravimetric analysis having water
loss of about 8% or more.
In general, the crystalline compound 5 is further characterized by Powder x-ray diffraction pattern substantially same as depicted in FIG.5, Differential Scanning

Calorimetry (DSC) substantially same as depicted in FIG.6 and Thermogravimetric Analysis (TGA) curve substantially same as depicted in FIG.7.
In another general aspect, there is provided substantially pure compound 5 or 5 hydrate thereof having a purity of about 98% or more, of about 99 % or more, of about 99.5% or more, of about 99.8% or more by weight by area percentage by HPLC.
In another general aspect, there is provided substantially pure compound 5 substantially free from one or more of compounds 4b or 4c,
jfY i\ AF
H2NAA ^NANArA
2 H
10 4b 4c
In general, the results of the analysis using HPLC of the compound 5 or hydrate thereof, prepared by the process of the present invention, has total impurities of about 0.5% or less, by weight by area percentage of HPLC and purity of about 99.5% or more, 15 by weight by area percentage of HPLC.
In another general aspect, there is provided compound 5b, which is a free base,
CH3
5b
In another general aspect, there is provided a free base compound 2, which is an 20 intermediate for the preparation of triaminopyrimidine compound 1.

CH3
CH3 rM
J. JL ,N-CH3
HN ^| HN N
V*
F 2
In another general aspect, there is provided a process for the preparation of compound 5, or a hydrate thereof,
CH3
" 1 HO NH2^^^>-7
5
5 the process comprising:
(a) reacting compound 4a with cyclopropane carboxylic acid in one or more solvents to
obtain compound 3 a;
CH3 CH3
N \fF N iTF
4a 3a
(b) reacting the compound 3a with diphenylmethanimine in one or more solvents to obtain
10 compound 2a;
CH3
Ph N*SrF
P1T^N^^>~7 2a
(c) reacting the compound 2a with hydrochloric acid in one or more solvents to obtain the
compound 5; and

CH3
N \fF
5
(d) obtaining the compound 5 or a hydrate thereof by crystallizing in one or more solvents.
In general, the hydrate of compound 5 obtained in step (e) is monohydrate. 5
In another general aspect, there is provided a process for the preparation of compound 5 or hydrate thereof wherein the intermediate compound 3a and 2a are not isolated. The reaction of compound 4 to compound 5 or hydrate thereof may be performed in single solvent in one-pot process. 10
In general, the compound 4 is reacted with cyclopropanecarboxylic acid to obtain compound 3a in the presence of an acid and an oxidizing agent, optionally in the presence of one or more solvents.
15 In general, the acid comprises one or more of sulfuric acid, hydrochloric acid,
hydrobromic acid, acetic acid, phosphoric acid, and trifluroacetic acid. In particular, sulfuric acid may be used.
The oxidizing agent is selected from the group consisting of mixture of ammonium 20 persulfate and silver nitrate.
In general, the solvent comprises one or more of methanol, ethanol, isopropanol,
butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, ethyl acetate, isopropyl
acetate, butyl acetate, methylene dichloride, toluene, xylene, ethylbenzene,
25 dimethylformamide, dimethylacetamide, dimethylsulfoxide, 1,4-dioxane, tetrahydrofuran,
acetonitrile and water or mixtures thereof. In particular, the solvent is water.
In general, the compound 3a is reacted with diphenylmethanimine to obtain compound 2a in the presence of a base, a catalyst and a ligand in one or more solvents. 30

In general, the base comprises one or more of sodium hydroxide, potassium hydroxide,
lithium hydroxide, barium hydroxide, sodium carbonate, potassium carbonate, barium
carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, STB, PTB,
pyridine, piperidine, morpholine, TEA, DIPEA, DBU, or DABCO. In particular, the STB
5 may be used.
In general, the catalyst comprises one or more of palladium(II)acetate, bis(triphenylphosphine) palladium(II)chloride, Tetrakis(triphynylphosphine)palladium(0), Tris(dibenzylideneacetone) dipalladium(O), Bis(dibenzylideneacetone) dipalladium(O), 10 Palladium(II) chloride. In particular, the palladium(II)acetate may be used.
In general, the ligand comprises BINAP, Xantphos, triphenylphosphine, tributylphosphine and XPhos. In particular, BINAP may be used.
15 In general, the solvent comprises one or more of xylene, toluene, ethylbenzene,
methylene dichloride, ethylene dichloride, chloroform, carbon tetrachloride, ethyl acetate, butyl acetate, and isopropyl acetate or mixtures thereof. In particular, the solvent toluene may be used.
20 In general, the compound 3a is reacted with diphenylmethanimine in the presence of an
amine protecting reagent to obtain pure compound 2a. The presence of an amine protecting reagent resulted in the reduction of impurity formation. The amine protecting agent is selected from tert-butyl dicarbonate (BOC), fluorenylmethyloxycarbonyl chloride (Fmoc-Cl), 9-fluorenylmethylsuccinimidyl carbonate (Fmoc-OSu), benzyl chloroformate (Cbz-
25 CI), dibenzyl pyrocarbonate (Cbz anhydride), acetyl chloride, acetic anhydride, trifluoroacetic anhydride (TFAA), phthalic anhydride, benzyl chloride, triphenylmethyl chloride and tosyl chloride. In particular, tert-butyl dicarbonate may be used.
In general, the compound 2a is reacted with hydrochloric acid to obtain compound 5 in 30 one or more solvents.
In general, the solvent comprises one or more of methanol, ethanol, isopropanol, butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, ethyl acetate, isopropyl
1 Q

acetate, butyl acetate, methylene dichloride, toluene, xylene, ethylbenzene, dimethylforraamide, dimethylacetamide, dimethylsulfoxide, 1,4-dioxane, tetrahydrofuran, acetonitrile, water or mixtures thereof. In particular, the solvent is a mixture of water and toluene. 5
In general, the compound 5 may be extracted with one or more solvents. The compound 5 as monohydrate may be obtained by the removal of solvents after extraction and crystallizing the residue with one or more solvents.
10 The solvent for extraction is selected from methylene dichloride, toluene, ethyl acetate,
methyl tert-butyl ether, 1,4-dioxane, cyclohexane, diethylether, tetrahydrofuran, or mixtures thereof. In particular, the reaction mixture is extracted with methylene dichloride.
In general, the compound 4a is reacted with cyclopropanecarboxylic acid in the
15 presence of sulphuric acid and ammonium persulfate and silver nitrate in water to obtain
compound 3a. The compound 3a is reacted with diphenylmethanimine in presence of
BINAP, palladium acetate, STB in toluene which is further reacted with cone. HC1 to
obtain compound 5 and isolated as hydrate. In particular, as monohydrate.
20 In general, wherever not specified, the reaction may be performed in the presence of
one or more solvents comprises of water, alcohols selected from methanol, ethanol, isopropanol, and n-butanol; esters selected from ethyl acetate, isopropyl acetate, t-butyl acetate, and isobutyl acetate; ketones selected from acetone, methyl ethyl ketone and methyl isobutyl ketone; hydrocarbons selected from toluene, xylene, ethyl benzene,
25 heptane, hexane, and cyclohexane; halogenated hydrocarbons selected from methylene dichloride, ethylene dichloride, chlorobenzene, chloroform, and carbon tetrachloride; nitriles selected from acetonitrile and propanenitrile.
In another general aspect, there is provided a process for crystallizing the intermediate 30 compounds of the present invention and avoid flash chromatography techniques reported in the prior art.

In another general aspect, there is provided a process for the preparation of compound 6,
H3C ,CH3
9
NH2 6
the process comprising:
5 (a) halogenating 2-butenenitrile to obtain compound 2,3-dihalobutanenitrile; and
(b) reacting the compound 2,3-dihalobutanenitrile with methyl hydrazine or salts thereof in
the presence of a base to obtain compound 6.
In general, the compound 2-butenenitrile may be halogenated via halogenation
10 comprises chlorination, bromination, fluorination, or iodination. In particular, the
halogenation comprises bromination using a brominating agent selected from bromine,
HBr-Acetic acid, 1,4-dibromo-dimethylhydantoin, N-bromosuccinimide. In particular,
bromine may be used.
15 In general, the compound 2,3-dihalobutanenitrile obtained in step (a) is 2,3-
dibromobutanenitrile.
In general, the compound 2,3-dibromobutanenitrile is further reacted with methyl hydrazine sulphate in the presence of a base to obtain a compound 6. 20
In general, the base comprises one or more of sodium hydroxide, potassium hydroxide,
lithium hydroxide, barium hydroxide, sodium carbonate, potassium carbonate, barium
carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, STB, PTB,
pyridine, piperidine, morpholine, TEA, DIPEA, DBU, or DABCO. In particular, the
25 sodium hydroxide may be used.
In general, wherever not specified, the reaction may be performed in the presence of
one or more solvents comprises of water, alcohols selected from methanol, ethanol,
isopropanol, and n-butanol; esters selected from ethyl acetate, isopropyl acetate, t-butyl
30 acetate, and isobutyl acetate; ketones selected from acetone, methyl ethyl ketone and
7(1

methyl isobutyl ketone; hydrocarbons selected from toluene, xylene, ethyl benzene, heptane, hexane, and cyclohexane; halogenated hydrocarbons selected from methylene dichloride, ethylene dichloride, chlorobenzene, chloroform, and carbon tetrachloride; nitriles selected from acetonitrile and propanenitrile. 5
In another general aspect, there is provided a triaminopyrimidine compound 1 having a purity of about 98% or more, of about 99% or more, of about 99.5% or more, of about 99.8% or more, by weight by area percentage by HPLC.
10 In another general aspect, there is provided a triaminopyrimidine compound 1 having a
purity of about 99% or more and total impurities of about 1.0% or less, by area percentage of HPLC.
In another general aspect, there is provided a triaminopyrimidine compound 1 having a 15 purity of about 99.6% or more, and total impurities of about 0.4% or less, by area percentage of HPLC.
In general, the triaminopyrimidine compound 1 is substantially free from one or more of compound 11, compound 12, compound 13, compound 14 and compound 15 as below:
20 Compound 11: (R)-N2,N4-bis(l,5-dimethyl-lH-pyrazol-3-yl)-5-(3,4-dimethyl- piperazin-
l-yl)pyrimidine-2,4-diamine;
Compound 12: (R)-N2,N4-bis(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-5-(3,4-
dimethy lpiperazin-1 -yl)pyrimidine-2,4-diamine;
Compound 13: (R)-4-cyclopropyl-6-((4-((l,5-dimethyl-lH-pyrazol-3-yl)amino)-5-(3,4-25 dimethylpiperazin-l-yl)pyrimidin-2-yl)amino)-3-fluoro-2-methylpyridine 1-oxide;
Compound 14: (R)-N4-( 1,5-dimethyl-1 H-pyrazol-3-yl)-5-(3,4-dimethylpiperazin-1 -yl)-
N -(5-fluoro-6-methylpyridin-2-yl)pyrimidine-2,4-diamine;
Compound 15: (S)-N2-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4-(l,5-dimethyl-lH-pyrazol-3-yl)-5-(3,4-dimethylpiperazin-l-yl)pyrimidine-2,4-diamine. 30
In another general aspect, there is provided substantially pure triaminopyrimidine compound 1 substantially free from one or more of the following compounds:

CH3 Y
N NH l A
H3C CH3 11 12 ^A^CH
CH3 v T
CH3 rM
CH3 rj'0 CH3
CH3 f=K T_^As^nH Ch|3 r^
H r 1 jL ,N-CH3 V T Uh3 H,c ! L ,N-CH3
^N^ HN-^N 3 F ^N^ HN-^N
W»ys 13 ^SAN
In general, the results of the analysis using HPLC of the compounds prepared by the process of the present invention has total impurities of about 1.0% or less, particularly 5 of about 0.8% or less, more particularly, more particularly about 0.4% or less, still more particularly about 0.2% or less, by area percentage of HPLC and having a purity of about 99% or more, particularly of about 99.2% or more, more particularly of about 99.6% or more, still more particularly of about 99.8% or more, by weight by area percentage of HPLC. 10
In another general aspect, there is provided a composition comprising triaminopyrimidine compound 1 having a purity of about 99% or more by weight, and (S)-N2-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4-(l,5-dimethyl-lH-pyrazol-3-yl)-5-(3,4-dimethylpiperazin-l-yl)pyrimidine-2,4-diamine (relative to triaminopyrimidine) in an 15 amount of about 0.5% or less, by weight, by area percentage of HPLC.

In general, the composition comprises (S)-N2-(4-cyclopropyl-5-fluoro-6-
methylpyridin-2-yl)-N4-(l,5-dimethyl-lH-pyrazol-3-yl)-5-(3,4-dimethyl piperazin-1-
yl)pyrimidine-2,4-diamine relative to triaminopyrimidine compound 1 in an amount of
about 0.25% or less, or of about 0.20% or less, or of about 0.15% or less, by weight by
5 area percentage of HPLC.
In another general aspect, there is provided a composition comprising:
triaminopyrimidine compound 1 having a purity of greater than or equal to 99% by weight,
and (R)-4-cyclopropyl-6-((4-((l,5-dimethyl-lH-pyrazol-3-yl)amino)-5-(3,4-
10 dimethylpiperazin-l-yI)pyrimidin-2-yl)amino)-3-fluoro-2-methylpyridine 1-oxide present (relative to triaminopyrimidine) in an amount of about 0.5% or less by weight, by area percentage of HPLC.
In general, the composition comprises (R)-4-cyclopropyl-6-((4-(( 1,5-dimethyl- 1H-15 pyrazol-3 -yl)amino)-5 -(3,4-dimethy lpiperazin-1 -yl)pyrimidin-2-yl)amino)-3 -fluoro-2-
methylpyridinc 1-oxide relative to triaminopyrimidine compound 1 in an amount of about 0.25% or less, or of about 0.20% or less, or of about 0.15% or less, by weight by area percentage of HPLC.
20 In general, the compositions as herein above comprising triaminopyrimidine
compound 1 having a purity of about 98% or more, of about 99% or more, of about 99.5% or more, of about 99.8% of more, of about 99.9% or more, by weight by area percentage of HPLC.
25 In another general aspect, there is provided a process for the preparation of
triaminopyrimidine compound 1, the process comprising: (a) reacting compound 10 with compound 9 in one or more solvents to obtain a compound 8;
CH3
9 Boc'N^i O
^ BYT °NTV
^.NH N^O SAo
^ H H
10 9 8

(b) reacting the compound 8 with a chlorinating agent in one or more solvents to obtain
compound 7;
CH3 B0C-N^) CI
V™
7
(c) reacting the compound 7 with compound 6 in one or more solvents in the presence of
5 a base to obtain compound 4;
PH3
H3C ,CH3 CH3 p=6 kNACI
4
(d) reacting the compound 4 with compound 5 in one or more solvents in the presence of
a base to obtain compound 3 or solution thereof;
m
CH3 f=*N if T i
HCI. NH/^^N^ N NH
5 jT"N
V7T CH3 v F
3
10 (e) reacting the compound 3 or solution thereof with an acid in one or more solvents to obtain compound 2 free base;

CH3
CH3 r«A
JL A -N-CH3
V^V-CH3
2
(f) reacting the compound 2 free base with a methylating agent in one or more solvents
to obtain the compound 1; and
(g) optionally crystallizing the compound in one or more solvents.
5
In general, the compound 10 and the compound 9 can be reacted in the presence of one or more solvents and a base.
Base comprises one or more of sodium hydroxide, potassium hydroxide, lithium
10 hydroxide, barium hydroxide, sodium carbonate, potassium carbonate, barium carbonate,
cesium carbonate, sodium bicarbonate, potassium bicarbonate, pyridine, piperidine,
morpholine, TEA, DIPEA, DBU, STB, PTB, or DABCO. In particular, pyridine, TEA or
DIPEA may be used.
15 Solvent comprises one or more of methanol, ethanol, isopropanol, butanol, acetone,
methyl ethyl ketone, methyl isobutyl ketone, ethyl acetate, isopropyl acetate, butyl acetate, methylene dichloride, toluene, xylene, ethylbenzene, dimethylformamide, dimethylacetamide, dimethylsulfoxide, 1,4-dioxane, tetrahydrofuran, acetonitrile, pyridine, TEA, DIPEA and water, or mixtures thereof. In particular, pyridine is used.
20
Preferably, the reaction of the compound 10 and the compound 9 is carried out at about 40 °C to about reflux temperature of the solvent. In particular, the reaction may be carried out at 110 to 115° C in pyridine for 5 to 15 hours to obtain compound 8.
25 In general, the compound 8 is reacted with a chlorinating reagent in the presence of a
catalyst in one or more solvents. The chlorinating reagent is selected from phosphorus

oxychloride, phosphorus pentachloride, phosphorus trichloride, and thionyl chloride. In particular, phosphorus oxychloride may be used.
In general, the catalyst is selected from the group consisting of pyridine, N,N-5 dimethyl aniline, N,N-diethyl aniline, and DIPEA. In particular, pyridine is used.
In general, the solvent comprises one or more of xylene, toluene, ethylbenzene, methylene dichloride, ethylene dichloride, chloroform, carbon tetrachloride, ethyl acetate, butyl acetate, isopropyl acetate, phosphorus oxychloride and thionyl chloride or mixtures 10 thereof. In particular, the solvent is phosphorus oxychloride.
In general, the compound 8 is reacted with phosphorus oxychloride in the presence of catalytic amount of pyridine at 110 to 115° C to obtain intermediate compound which is then reacted with one or more protecting agent selected from tert-butyl dicarbonate (BOC),
15 fluorenylmethyloxycarbonyl chloride (Fmoc-Cl), 9-fluorenylmethylsuccinimidyl carbonate (Fmoc-OSu), benzyl chloroformate (Cbz-Cl), dibenzyl pyrocarbonate (Cbz anhydride), acetyl chloride, acetic anhydride, Trifluoroacetic anhydride (TFAA), phthalic anhydride, benzyl chloride, triphenylmethyl chloride, tosyl chloride, in one or more solvent to obtain compound 7. In particular, tert-butyl dicarbonate may be used.
20
In general, the solvent for the preparation of compound 7 comprises one or more of methanol, ethanol, isopropanol, butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, ethyl acetate, isopropyl acetate, butyl acetate, methylene dichloride, chloroform, toluene, water, or mixtures thereof. In particular, the mixture of ethyl acetate and water
25 may be used.
The compound 7 may be isolated by removing ethyl acetate from the reaction mixture and treating with one or more alcohols selected from methanol, ethanol, isopropanol, butanol or water, or mixtures thereof. 30
The reaction of compound 7 with compound 6 is carried out in the presence of a base in one or more solvents.

In general, the base comprises one or more of sodium hydroxide, potassium
hydroxide, lithium hydroxide, barium hydroxide, sodium carbonate, potassium carbonate,
barium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, pyridine,
piperidine, morpholine, TEA, DIPEA, DBU, STB, PTB, or DABCO. In particular, the
5 cesium carbonate may be used.
In general, the solvent comprises one or more of methanol, ethanol, isopropanol,
butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, ethyl acetate, isopropyl
acetate, butyl acetate, methylene dichloride, toluene, xylene, ethylbenzene,
10 dimethylformamide, dimethylacetamide, dimethylsulfoxide, 1,4-dioxane, tetrahydrofuran,
acetonitrile and water, or mixtures thereof. In particular, the solvent is toluene.
In general, the reaction of compound 7 with compound 6 may be performed in the
presence of catalyst optionally in presence of a ligand. The catalyst comprises one or more
15 of Palladium(II)acetate, bis(triphenylphosphine)palladium(II)chloride,
Tetrakis(triphenylphosphine)palladium(0), tris(dibenzylideneacetone) dipalladium(O), bis(dibenzylideneacetone)dipalladium(0), Palladium(II)chloride. The ligand comprises BINAP, Xantphos, triphenylphosphine, tributylphosphine and Xphos.
20 In general, the compound 7 is reacted with compound 6 in toluene and in the
presence of cesium carbonate, palladium(II)acetate and BINAP at 110 to 115 °C for 5 to 20 hours, in particular for 16 hours to obtain the compound 4.
In general, the pure compound 4 can be isolated from the reaction mixture by 25 adsorbing the crude solution on silica gel followed by removal of solvent and extraction with ethyl acetate, hexane, or mixtures thereof.
In general, the compound 4 is reacted with compound 5 as monohydrate in one or more solvents in the presence of a base to obtain compound 3. 30
In general, the base comprises one or more of sodium hydroxide, potassium hydroxide, lithium hydroxide, barium hydroxide, sodium carbonate, potassium carbonate, barium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, STB,

PTB, pyridine, piperidine, morpholine, TEA, DIPEA, DBU, or DABCO. In particular, the cesium carbonate may be used.
In general, the solvent comprises one or more of methanol, ethanol, isopropanol,
5 butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, ethyl acetate, isopropyl
acetate, butyl acetate, methylene dichloride, toluene, xylene, ethylbenzene,
dimethylformamide, dimethylacetamide, dimethylsulfoxide, 1,4-dioxane, tetrahydrofuran,
acetonitrile and water, or mixtures thereof. In particular, the solvent is toluene.
10 In general, the reaction of the compound 4 with the compound 5 or a hydrate
thereof may be performed in the presence of catalyst optionally in presence of ligand. The
catalyst comprises one or more of palladium(II)acetate, bis(triphenylphosphine)
palladium(II)chloride, tetrakis(triphenylphosphine)palladium(0), tris(dibenzyli-
deneacetone)dipalladium(O), bis(dibenzylideneacetone)dipalladium(0),
15 Palladium(II)acetate. The ligand comprises BINAP, Xantphos, triphenyl- phosphine, tributylphosphine and Xphos.
In general, the compound 4 is reacted with the compound 5 monohydrate in the presence of cesium carbonate in the presence of palladium acetate and BINAP in toluene 20 at 110 to 115 °C for 7 to 15H to obtain the compound 3.
In general, the pure compound 3 is isolated from reaction mixture by adsorbing compound 3 on silica gel followed by removal of solvent and extraction with ethyl acetate.
25 In general, the solution containing compound 3 obtained in step (d) can be used in
step (R) without further purification or treatment or isolation.
In general, the compound 3 or solution thereof is further reacted with an acid to obtain free base compound 2. The reaction may be performed in the presence of one or 30 more solvents.

In general, the acid comprises one or more of hydrochloric acid, hydrobromic acid, acetic acid, phosphoric acid, and trifluroacetic acid. In particular, hydrochloric acid may be used.
5 In general, the solvent comprises one or more of methanol, ethanol, isopropanol,
butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, ethyl acetate, isopropyl acetate, butyl acetate, methylene dichloride, toluene, xylene, ethylbenzene, dimethylformamide, dimethylacetamide, dimethylsulfoxide, 1,4-dioxane, tetrahydrofuran, acetonitrile and water or mixtures thereof. In particular, the solvent is mixture containing 10 toluene and water.
In general, the compound 3 is reacted with cone, hydrochloric acid in water at
room temperature to about reflux temperature of solvent for sufficient time. The reaction
mixture may be basified and extracted with one or more solvents to obtain compound 2.
15 The compound 2 as free base may be obtained by removal of solvent after extraction and
crystallizing the residue with one or more solvents.
In general, the reaction of compound 3 with cone, hydrochloric acid may be done at 25 °C to about 120 °C. In particular, at about 50 °C to about 55 °C. 20
The reaction mixture may be basified using one or more base selected from sodium hydroxide, potassium hydroxide, lithium hydroxide, barium hydroxide, sodium carbonate, potassium carbonate, barium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate. In particular, sodium carbonate may be used. 25
The solvent for extraction is selected from methylene dichloride, toluene, ethyl acetate, methyl tert-butyl ether, 1,4-dioxane, and tetrahydrofuran or mixtures thereof. In particular, the reaction mixture is extracted with methylene dichloride.
30 In general, there is provided a process for crystallizing free compound 2
comprising crystallizing from one or more solvents.

In general, the solvent for crystallization of compound 2 free base comprises one or
more of methanol, ethanol, isopropanol, butanol, acetone, methyl ethyl ketone, methyl
isobutyl ketone, isopropyl acetate, butyl acetate, dimethylformamide, dimethylacetamide,
dimethylsulfoxide, acetonitrile, water, or mixtures thereof. In particular, the solvent is
5 acetonitrile.
In another general aspect, there is provided a process for preparation of triaminopyrimidine compound 1 comprising methylating free base compound 2 with a methylating agent, optionally in the presence of a reducing agent, in one or more solvents. 10
In general, the compound 2 free base is reacted with methylating agent, optionally in the presence of a reducing agent, in one or more solvents to obtain the compound 1.
In general, the methylating agent comprises one or more of diazomethane, 2,2-
15 dimethoxypropane, dimethyl carbonate, dimethyl sulfide, dimethyl zinc, methyl flouro
sulfonate, methyl iodide, methyl bromide, methyltrifluoro methane sulfonate,
trimethoxonium tetraflouroborate, formaldehyde, and mixture of formic acid and
formaldehyde. In particular, the methylating agent is formaldehyde.
20 In general, the reducing agent comprises one or more of sodium cyanoborohydri-
de, sodium triacetoxyborohydride, lithium aluminium hydride, and sodium borohydride.
In general, the compound 2 free base is reacted with formaldehyde in the presence of sodium triacetoxyborohydride to obtain the compound 1. 25
In general, the compound 2 free base is reacted with formaldehyde in the presence
sodium triacetoxyborohydride to obtain compound 1. The reaction mixture may be
basified and extracted with one or more solvents to obtain compound 1. The compound 1
may be obtained by removal of solvent after extraction and crystallizing the residue with
30 one or more solvents.
In general, the reaction of compound 2 free base with formaldehyde may be done at 25 °C to about 120 °C. In particular, at about 25 °C to about 35 °C.

The reaction mixture may be basified using one or more base selected from sodium hydroxide, potassium hydroxide, lithium hydroxide, barium hydroxide, sodium carbonate, potassium carbonate, barium carbonate, cesium carbonate, sodium bicarbonate, and 5 potassium bicarbonate. In particular, sodium carbonate may be used.
The solvent for extraction is selected from methylene dichloride, toluene, ethyl acetate, methyl tert-butyl ether, 1,4-dioxane, and tetrahydrofuran or mixtures thereof. In particular, the reaction mixture is extracted with methylene dichloride. 10
In general, the solvent for crystallization of compound 1 comprises one or more of
methanol, ethanol, isopropanol, butanol, acetone, methyl ethyl ketone, methyl isobutyl
ketone, isopropyl acetate, butyl acetate, dimethylformamide, dimethylacetamide,
dimethylsulfoxide, acetonitrile, water or mixtures thereof. In particular, the solvent is
15 acetonitrile.
In another general aspect, there in provided a process for the preparation of crystalline form of triaminopyrimidine compound 1, the process comprises crystallizing the triaminopyrimidine compound 1 in one or more solvents. 20
In general, the triaminopyrimidine compound 1 prepared during the reaction may
be crystallized in-situ by solvent exchange or addition and removal of solvents or in the
presence of another solvent, or triaminopyrimidine compound 1 may be isolated as solid
compound and further crystallized in one or more solvents to obtain their solid state forms.
25
In general, the crystalline Form I of triaminopyrimidine compound 1 may be
obtained by crystallization in one or more of methanol, ethanol, isopropanol, butanol,
acetone, methyl ethyl ketone, methyl isobutyl ketone, isopropyl acetate, butyl acetate,
dimethylformamide, dimethylacetamide, dimethylsulfoxide, acetonitrile, water or mixtures
30 thereof. In particular, acetonitrile may be used,
In general, the crystalline Form II of triaminopyrimidine compound 1 may be obtained by crystallization in one or more methanol, ethanol, isopropanol, butanol,

acetone, methyl ethyl ketone, methyl isobutyl ketone, isopropyl acetate, butyl acetate, dimethylformamide, dimethylacetamide, dimethylsulfoxide, acetonitrile, water or mixtures thereof. In particular, mixture of acetonitrile and water.
5 In general, the solvent for crystallization of compound 2 free base comprises one or
more of methanol, ethanol, isopropanol, butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, isopropyl acetate, butyl acetate, dimethylformamide, dimethylacetamide, dimethylsulfoxide, acetonitrile, water or mixtures thereof. In particular, the solvent is acetonitrile.
10
In general, wherever not specified, the reaction may be performed in presence of one or more solvents comprises of water, alcohol selected from methanol, ethanol, isopropanol, and n-butanol; ester selected from ethyl acetate, isopropyl acetate, t-butyl acetate, and isobutyl acetate; ketone selected from acetone, methyl ethyl ketone and
15 methyl isobutyl ketone; hydrocarbon selected from toluene, xylene, ethyl benzene, heptane, hexane, and cyclohexane; halogenated hydrocarbon selected from methylene dichloride, ethylene dichloride, chlorobenzene, chloroform, and carbon tetrachloride; nitriles selected from acetonitrile and propanenitrile.
20 In another general aspect, there is provided a process for crystallizing the
intermediate compounds of the present invention and avoid flash chromatography techniques reported in the prior art.
In further general aspect, there is provided a process for the preparation of 25 triaminopyrimidine compound 1 of the present invention in crystalline form thereby avoiding microwave irradiation conditions as reported in the prior art.
In another general aspect, there is provided a pharmaceutical composition comprising crystalline triaminopyrimidine compound 1 and pharmaceutically acceptable 30 carrier, diluents and excipients.

In another general aspect, there is provided a pharmaceutical composition comprising substantially pure triaminopyrimidine compound 1 and one or more of pharmaceutically acceptable carrier, diluents and excipients.
5 In general, the pharmaceutical composition of the present invention may be in the
form of a solid or liquid dosage forms for oral, parenteral or topical use and may have immediate or sustained release characteristics. The dosage forms possible include powders, granules, creams, tablets, capsules, injectable, solutions, elixirs or suspensions.
10 Powder X-ray diffraction of triaminopyrimidine compound 1 and compound 5 can
be obtained under following conditions.
The powder x-ray diffraction pattern can be measured using PANalytical ExpertPro x-ray diffractometer, or Bruker, or Philips, or any other equivalent make, and having 15 CuKa source.
Differential Scanning Calorimetry of triaminopyrimidine compound 1 and compound 5 can be obtained under following conditions.
20 Differential scanning calorimetric analysis can be performed using a Perkin Elmer
DSC control unit and a DSC 300 °C differential scanning calorimeter. 2-5 mg samples were placed in crimped aluminum pans and heated from 50 °C to 300 °C in a liquid nitrogen atmosphere at a heating rate of 10 °C/minute. Zinc-Indium or any other suitable reference may be as the standard substance.
25
The examples are set forth to aid in understanding the invention but are not intended to, and should not be construed to limit its scope in any way. The examples do not include detailed descriptions of conventional methods. Such methods are well known to those of ordinary skill in the art and are described in numerous publications.
30
Examples: Preparation of Intermediates
Example-1: Preparation of 6-chloro-4-cyclopropyl-3-fluoro-2-methylpyridine

In a 250 mL 4N round bottom flask, process water (30 ml) and cyclopropanecarboxylic acid (14.19 g, 164.88 mmol) were added at 25 to 35°C and started stirring. Sulphuric acid (4.4 ml, 82.44 mmol) was charged to the reaction mixture. Silver nitrate (4.18 g, 24.73 mmol), 6-Chloro-3-fluoro-2-methyIpyridine (6 g, 41.22 mmol) were 5 charged to the reaction mixture. Aqueous solution of ammonium persulphate (65.85 g, 288.54 mmol in 90 mL water) was added to the reaction mixture in 30 to 60 min at temperature NMT 60 °C. After the completion of the reaction as monitored by HPLC, toluene (30 ml) was added to the reaction mixture and stirred for 15 min. The reaction mixture filtered, separated layers from filtrate and extracted aqueous layer using toluene 10 (30 mL). The organic layer was washed with aqueous sodium carbonate solution (30 mL) and water. The organic layer was distilled completely under vacuum at 60 °C to obtain 3.37 g syrupy mass as titled compound.
Example-2: Preparation of 6-chloro-4-cyclopropyl-3-fluoro-2-methylpyridine
15 In a suitable glass assembly, process water (7.5 L) and cyclopropanecarboxylic
acid (3.55 Kg, 41.24 mol) were added at 25 to 35 °C and stirred. Sulphuric acid (2.02 Kg, 20.59 mol), silver nitrate (1.05 Kg, 6.21 mol), 6-chloro-3-fluoro-2-methylpyridine (1.5 Kg, 10.3 mol) were added to the reaction mixture. Aqueous solution of ammonium persulphate (16.46 g, 72.13 mmol in 22.5 L water) was added to the reaction mixture at 55 to 60 °C
20 and maintained. After the completion of the reaction as monitored by HPLC, toluene (7.5 L) was added to the reaction mixture and stirred for 15 min. The reaction mixture was filtered, organic layer was separated and aqueous layer was extracted using toluene (6 L), filtered the reaction mixture and washed the solid with toluene (1.5 L). The combined organic layer was washed with 20% sodium carbonate solution (9 L) and water. The
25 organic layer was concentrated completely under vacuum at 60 °C to obtain 880 g (86.50%) syrupy mass of titled compound.
ExampIe-3: Preparation of N-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yi)-l,l-diphenyl-methanimine
30 In a 100 mL 3N round bottom flask, 6-chloro-4-cyclopropyl-3-fluoro-2-
methylpyridine (2.69 g, 14.48 mmol) and toluene (30 mL) were added at 25 to 35 °C. Diphenylmethanimine (3.15 g, 17.38 mmol) was charged to the reaction mixture and stirred for 5-10 min under nitrogen purging. Racemic BINAP (270 mg, 0.43 mmol) and

palladium acetate (98 mg, 0.43 mmol) were added to the reaction mixture. Sodium-tert-butoxide (2.78 g, 28.96 mmol) was added to the reaction mixture and heated to 100 to 110° C under nitrogen. After the completion of the reaction as monitored by HPLC, the reaction mixture was cooled to 25 to 35 °C and filtered over hyflo bed and washed with toluene. 5 The filtrate containing titled compound was preserved for next stage of reaction.
ExampIe-4: Preparation of N-(4-cyclopropyI-5-fluoro-6-methylpyridin-2-yl)-l,l-diphenyl-methanimine
In a suitable assembly, 6-chloro-4-cyclopropyl-3-fluoro-2-methylpyridine (880) 10 and toluene (7.5 L) were added at 25 to 35 °C. Diphenylmethanimine (787 g, 4.34 mmol) and BOC anhydride (237 g, 1.086 mol) was added to the reaction mixture and stirred for 5-10 min under nitrogen purging. Racemic BINAP (67.6 g, 0.108 mmol) and palladium acetate (24.4 g, 0.108 mol) were added to the reaction mixture. Sodium-fcrt-butoxide (870 g, 9.05 mol) was added to the reaction mixture and heated to 100 to 110 °C under nitrogen. 15 After the completion of the reaction as monitored by HPLC, the reaction mixture was cooled to 25 to 35 °C, water (6 L) was added. The reaction mixture was filtered over hyflo bed and washed with toluene. The fdtrate containing titled compound was preserved for next stage of reaction.
20 ExampIe-5: Preparation of 4-cyclopropyl-5-fluoro-6-methylpyridin-2-amine hydrochloride monohydrate
In a 100 raL 3N round bottom flask, N-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-1,1 -diphenylmethanimine in toluene as obtained in example-3 was added water (25 mL) at 25 to 35° C. The cone. HC1 (3 mL) was charged to the reaction mixture and heated
25 to 40 to 50 °C. After the completion of the reaction as monitored by HPLC, the reaction mixture was cooled to 25 to 35 °C. Layers were separated. The aqueous layer was treated with methylene dichloride and pH was adjusted to 7.5 to 8.5 using sodium carbonate solution, stirred for 15 min and layers were separated. Aqueous layer was extracted with methylene dichloride, charcoaled and acidified to pH 3 to 4 with aqueous HC1. The solvent
30 was distilled completely and acetonitrile (9 mL) and ethyl acetate (9 mL) was added. The reaction mixture was stirred for 1 hour at 25 to 35° C. The product was filtered and washed with ethyl acetate. The product was dried at 50° C for 4 hours under vacuum to obtain 1.62 g title compound as monohydrate yellow crystalline solid having 99.51% HPLC purity.

ExampIe-6: Preparation of 4-cycIopropyI-5-fluoro-6-methyIpyridin-2-amine hydrochloride monohydrate
In a suitable glass assembly, N-(4-cyclopropyl-5-fIuoro-6-methylpyridin-2-yl)-l,l-5 diphenylmethanimine in toluene as obtained in example-4 was added water (6 L) at 25 to 35° C. The cone. HC1 (750 mL) was charged to the reaction mixture and heated to 40 to 50 °C. After the completion of the reaction as monitored by HPLC, the reaction mixture was cooled to 25 to 35 °C. Layers were separated. The aqueous layer was treated with methylene dichloride (3 L) and pH was adjusted to 7.5 to 8.5 using sodium carbonate
10 solution, stirred for 15 min and layers were separated. Aqueous layer was extracted with methylene dichloride (3 L), charcoaled and acidified to pH 3 to 4 with aqueous HC1. The solvent was distilled completely and acetonitrile (1.5 L) and ethyl acetate (1.5 L) were added. The reaction mixture was stirred for 1 hour at 25 to 35° C. The product was fdtered and washed with ethyl acetate. The product was dried at 50° C for 4 hours under vacuum
15 to obtain 489 g (96.80%) title compound as monohydrate yellow crystalline solid having 99.51% HPLC purity. The crystalline compound is characterized by Powder x-ray diffraction pattern (FIG.5), Differential scanning calorimetry (FIG.6) and Thermogravimetric analysis (FIG.7).
20 Example 7: Preparation of 2,3-dibromobutanenitrile
In a 2 L round bottom flask, dichloromethane (550 mL) and 2-butenenitrile 110 g (1.64 mol) were cooled to 20 to 25 °C. A solution of bromine 275 g (1.72 mol) in dichloromethane (220 mL) was dropwise added at 20 to 25 °C. Hydrobromic acid 1.43 ml (0.0082 mol) in acetic acid (33%) solution was added into the reaction mixture and stirred 25 for 4 hours. After the completion of reaction, Na2S203 (550 mL) 4% aqueous solution was added and the reaction mixture was stirred for 15 min. The separated organic layer was distilled under vacuum completely to obtain 364.2 g (97.9%) of title compound as an oil.
Example 8: Preparation of l,5-dimethyl-lH-pyrazol-3-amine
30 In a 5 L round bottom flask, water (1. 36 L), sodium hydroxide 340 g (8.99 mol)
were added and the reaction mixture was cooled to 0 to 5°C. A solution of methyl hydrazine sulphate 237.8 g (1.65 mol) in 680 mL water was added dropwise to the reaction mixture and stirred below 10 °C. 2,3-dibromobutanenitrile 340 g (1.5 mol) prepared in

example-7 was added and the reaction mixture was stirred below 10 °C for 2 hours. After the completion of reaction, toluene (630 mL) was added and the reaction mixture was stirred for 15 min. The aqueous layer was separated and the organic layer was removed. The aqueous layer was extracted with dichloromethane (5.1 L). The combined organic 5 layer was distilled completely under vacuum to obtain residue. Diisopropyl ether (680 mL) was added and the reaction mixture was stirred at 0 to 5 °C for 1 hour. The reaction mixture was filtered, washed with diisopropyl ether and dried to obtained 121.5 g (72.93%) of title compound having 95.63% purity.
10 Examples: Preparation of triaminopyrimidine compounds
Example-9: Preparation of tert-butyl (R)-4-(2,4-dioxo-l,2,3,4-tetrahydro- pyrimidin-5-yl)-2-methylpiperazine-l-carboxylate
In 2 L four neck round bottom flask, 1.25 Kg (6.545 mol) 5-bromouracil, 1.87 Kg (9.360 mol) tert-butyl (R)-2-methylpiperazine-l-carboxylate and 5L pyridine were added 15 at 25 to 35° C. The reaction mass was stirred for 15 hours at 115 to 120°C. After completion, the reaction mass was cooled to 25 to 35°C. 12.5 L water was added and stirred for 1 hour. The reaction mass was filtered, washed with 2.5 L water and dried to obtain 1.37 Kg (67.4%) of title compound.
20 Example-10: Preparation of tert-butyl (R)-4-(2,4-dichloropyrimidin-5-yl)-2-methylpiperazine-1-carboxylate
In 20 L four neck round bottom flask, 1.36 Kg (4.382 mmol) tert-butyl (R)-4-(2,4-dioxo-l,2,3,4-tetrahydropyrimidin-5-yl)-2-methylpiperazine-l-carboxylate and 6.8 L phosphorus oxychloride were added at 25 to 35° C. 26.5 mL pyridine (0.329 mol) was
25 added and the reaction mass was heated to 105 to 110 °C and stirred for 4 hours. After the completion of the reaction, phosphorus oxychloride was distilled completely at atmospheric pressure. 2.72 L acetone was added and the reaction mixture was quenched into 4.08 L water. Acetone was removed by distillation under vacuum. 20% sodium carbonate solution was added to adjust pH 7.5-8.5 of the reaction mixture. 1.14 Kg (5.258
30 mol) di-tert-butyl dicarbonate and 9.52 L ethyl acetate were added and stirred for 2 hours at 25 to 35 °C. After the completion of the reaction, the organic layer was separated and aqueous layer was extracted with 6.8 L ethyl acetate. The combined ethyl layers were distilled to remove ethyl acetate completely under vacuum to obtain residue. 1.36 L

isopropyl alcohol was added to the residue and isopropyl alcohol was removed completely.
4.08 L isopropyl alcohol and 6.8 L water were added to the residue and stirred for 1 hour.
The reaction mass was filtered, washed with water and dried to obtain 1.25 Kg of title
compound. 5
Example-11: Preparation of tert-butyl (R)-4-(2-chloro-4-[(l,5-dimethyl-lH-pyrazol-3-
yl)amino)pyrimidin-5-yl]-2-methyIpiperazine-l-carboxylate
In 20 L round bottom flask, 640 g (1.843 mol) tert-butyl (R)-4-(2, 4-dichloropyrimidin-5-yl)-2-methylpiperazine-l-carboxylate, 225.3 g (2.027 g) 1,5-
10 dimethyl-lH-pyrazol-3-amine and 9.6L toluene were added at 25 to 35°C. 1.2 Kg (3.686 mol) cesium carbonate was added. The reaction mixture was purged for 15 min under nitrogen. 12.41 g (0.0553 mol) palladium acetate and 34.43 g (0.0553 mol) racemic 2,2'-bis(diphenylphosphino)-l,l'-binaphthyl were added and the reaction mass was maintained for 16 hours at 110 to 115 °C under nitrogen. After the completion of the reaction, the
15 reaction mixture was filtered through a celite bed and washed the bed with 1.28 L toluene. Toluene was distilled completely and 2.56 L dichlromethane was added. The compound was adsorbed by 1.92 Kg silica gel (60-120 mesh). The dichloromethane was distilled completely under vacuum and 12.8 L mixture of ethyl acetate and hexane was added to the residue and stirred for 2 hours. The silica gel was filtered and the filtrate was distilled
20 completely under vacuum to obtain 595 g title compound.
Example-12: Preparation of tert-butyl (R)-4-(2-((4-cyclopropyl-5-fluoro-6-methyIpyridin-2-yl)amino)-4-((l,5-dimethyl-lH-pyrazol-3-yl)amino) pyrimidin-5-yl)-2-methylpiperazine-l-carboxylate
25 In 20 L round bottom flask, 595 g (1.40 mol) tert-butyl (R)- 4-(2-chloro-4-[(l,5-
dimethyl-lH-pyrazol-3-yl)amino)pyrimidin-5-yl]-2-methylpiperazine-l-carboxylate, 305 g (1.38 mol) 4-cyclopropyl-5-fluoro-6-methylpyridin-2-amine hydrochloride and 11.5 L toluene were added at 25 to 35°C. 1.08 Kg (3.32 mol) cesium carbonate was added. The reaction mixture was purged for 15 min under nitrogen. 17.21 g (27.6 mmol) palladium
30 acetate and 6.21 g (27.6 mmol) racemic 2,2'-bis(diphenylphosphino)-l,P-binaphthyl were added. The reaction mass was stirred for 6 hours at 110 tol 15 °C under nitrogen. After the completion of the reaction, the reaction mixture was filtered through a celite bed and washed with toluene. The filtrate was used as such in the next step without further treatment.

Example-13: Preparation of tert-butyl (R)-4-(2-((4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)amino)-4-((l,5-dimethyI-lH-pyrazol-3-yl)amino) pyrimidin-5-yl)-2-methylpiperazine-l-carboxylate
5 In 500 mL four neck round bottom flask, 7.5 g (17.77 mmol) (R)-tert-butyl 4-(2-
chloro-4-[(l,5-dimethyl-lH-pyrazol-3-yl)amino)pyrimidin-5-yl]-2-methylpiperazine-l-carboxylate, 3.92 g (17.77 mmol) 4-cyclopropyl-5-fluoro-6-methylpyridin-2-amine hydrochloride compound and 150 mL toluene were added at 25 to 35 °C. 20 g (61.3 mmol) cesium carbonate was added. The reaction mixture was purged for 15 min under nitrogen.
10 Then, 130 mg (0.58 mmol) palladium acetate and 360 mg (0.58 mmol) racemic 2,2'-bis(diphenylphosphino)-l,l'-binaphthyl were added. The reaction mass was stirred for 18 hours at 110 to 115° C under nitrogen. After completion, the reaction mixture was fdtered through a celite bed and washed with toluene. The filtrate was used as such in the next step without further treatment.
15
Example-14: (R)-N2-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4-(l, 5-dimethyl-lH-pyrazol-3-yl)-5-(3-methylpiperazin-l-yl)pyrimidine-2,4-diamine
In 50 L glass assembly, the filtrate containing tert-butyl (R)-4-(2-((4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)amino)-4-((l,5-dimethyl-lH-pyrazol-3-yl)amino)
20 pyrimidin-5-yl)-2-methylpiperazine-l-carboxylate from example 13 was taken. 11.5 L water and 1.28 L Cone. HC1 were added at 25 to 35 °C. The reaction mass was stirred for 2 hours at 50 to 55 °C. After the completion of the reaction, reaction mixture was cooled to room temperature and filtered over celite bed and washed with water. The separated the aqueous layer from filtrate was basified by using 20% sodium carbonate solution and
25 extracted with 12.8 L methylene dichloride. The organic layer was distilled completely under vacuum to obtain residue. 9.6 L acetonitrile was added to the residue and heated to reflux for 30 min. The reaction mixture was cooled and stirred at 25 to 35 °C for 1 hour. The reaction mixture was filtered, washed with 640 mL acetonitrile and dried to obtain 360 g titled compound.
30
Example-15: (R)-N2-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4-(l,5-dintethyl-lH-pyrazol-3-yl)-5-(3,4-dimethylpiperazin-l-yl)pyrimidine-2,4-diamine

In 250 mL four neck round bottom flask, 4.7 g (10.4 mmol) (R)-N -(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4-(l,5-dimethyl-lH-pyrazol-3-yl)-5-(3,4-dimethylpiperazin-l-yl)pyrimidine-2,4-diamine was dissolved in 56 mL ethanol. 1.89 g (23.32 mmol) formaldehyde and 1.44 g (22.90 mmol) sodium cyanoborohydride were 5 added. Adjusted pH 5-6 using acetic acid and stirred the reaction mass at 25 to 35 °C for 2 hours. After completion, ethanol was distilled completely under vacuum. 47 mL water was added to the residue. The reaction mass was basified by 20% sodium carbonate solution and extracted with methylene dichloride. Both the organic layers were combined and distilled completely under vacuum. 94 mL acetonitrile was added to the residue and heated 10 to reflux for 15 min. The reaction mass was cooled to 25 to 35° C and stirred for 1 hour. The reaction mass was filtered, washed with 5 mL acetonitrile and dried to obtain 3.7 g title compound as crystalline solid, having HPLC purity of about 99.61%.
Example-16: (R)-N2-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4-(l,5-dimethyl-15 lH-pyrazol-3-yl)-5-(3,4-dimethylpiperazin-l-yl)pyrimidine-2,4-diamine
In 20 L round bottom flask, 725 g (1.60 mol) (R)-N2-(4-cyclopropyl-5-fluoro-6-methy lpyridin-2-y l)-N4-( 1,5 -dimethyl-1 H-pyrazol-3 -yl)-5 -(3,4-dimethy lpiperazine-1 -yl)pyrimidine-2,4-diamine was dissolved in 6.52 L dichloromethane. 261.5 g (3.2 mol) formaldehyde and 510.4 g (2.4 mol) sodium triacetoxyborohydride were added and stirred
20 the reaction mixture at 25 to 35 °C for 2 hours. After the completion of the reaction, 3.63 L water was added into the reaction mixture. The reaction mixture was basified by 20% sodium carbonate solution and the organic layer was separated. The aqueous layer was extracted with 1.45 L methylene dichloride. The combined organic layers were distilled completely under vacuum. 14.5 L acetonitrile was added to the residue and heated to
25 reflux for 15 min. The reaction mixture was cooled to 25 to 35° C and stirred for 1 hour. The reaction mass was filtered, washed with 1.45 L acetonitrile and dried to obtain 632 g of title compound as crystalline solid having 99.01% HPLC purity. The crystalline compound is characterized by Powder x-ray diffraction pattern (FIG.l) and Differential Scanning Calorimetry (FIG.2).
30
Example-17: Preparation of (R)-N2-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4~ (l,5-dimethyl-lH-pyrazol-3-yl)-5-(3,4-dimethylpiperazin-l-yl)pyrimidine-2,4-diamine

In a 10 mL round bottom flask, 300 mg (0.644 mmol) (R)-N2-(4-cyclopropyl-5-fluoro-6-methylpyridin-2-yl)-N4-( 1,5-dim ethyl-1 H-pyrazol-3 -yl)-5 -(3,4-dimethylpiperazin-l-yl)pyrimidine-2,4-diamine, 2.7 mL acetonitrile and 0.3 mL water were added and the reaction mixture was heated to reflux for 15 min. The reaction mixture 5 was cooled to 25 to 35 °C and stirred for 1 hour. The reaction mass was filtered, washed with acetonitrile and dried to obtain 201 mg (67%) title compound as crystalline solid. The crystalline compound is characterized by Powder x-ray diffraction pattern (FIG.3) and Differential Scanning Calorimetry (FIG.4).
10 While the present invention has been described in terms of its specific
embodiments, certain modifications and equivalents will be apparent to those skilled in the art and are intended to be included within the scope of the present invention.

We claim:
1. A crystalline Form I of triaminopyrimidine compound 1,
CH3
CH3 rM
IsT NH
fl N
X/*\ CH3
V F
1
characterized by powder x-ray diffraction pattern having peaks expressed in degrees 2θ at 6.3º and 9.8º± 0.2 2θ.
2. The crystalline Form I as claimed in claim 1, further characterized by powder x-ray diffraction pattern having peaks expressed in degrees 2θ at 6.3º, 7.8º, 9.8º, 15.4º, and 20.0º± 0.2 2θ; or a differential scanning calorimetry analysis having onset at 179 ºC± 5 ºC and endothermic peak at 181 ºC± 5 ºC.
3. A crystalline Form II of triaminopyrimidine compound 1,
CH3
CH3 r==N NH
fl N
X/^\ CH3
F 1
characterized by powder x-ray diffraction pattern having peaks expressed in degrees 2θ at 13.8º, 21.9º, and 23.7º± 0.2 2θ.
4. The crystalline Form II as claimed in claim 3, further characterized by
(i) powder x-ray diffraction pattern having peaks expressed in degrees 2θ at 8.3º, 12.1º, 13.8º, 21.9º, and 23.7º± 0.2 2θ; or
(ii) a differential scanning calorimetry analysis having atleast one onset at 161 ºC± 5 ºC and endothermic peak at 163 ºC± 5 ºC; or

(iii) a differential scanning calorimetry having one onset at 161 ºC± 5 ºC and second onset at 179 ºC± 5 ºC; or
(iv) a differential scanning calorimetry having one endothermic peak at 163 ºC± 5 ºC and second endothermic peak at 181 ºC± 5 ºC.
5. The crystalline Form I of triaminopyrimidine compound 1 as claimed in claim 1 or crystalline Form II of triaminopyrimidine compound 1 as claimed in claim 3, having a purity of 98% or more when measured by area percentage by HPLC.
6. The crystalline Form I of triaminopyrimidine compound 1 as claimed in claim 1 or crystalline Form II of triaminopyrimidine compound 1 as claimed in claim 3, having 1% or less by area percentage of HPLC, each of the compound 11, compound 12, compound 13, compound 14 and compound 15,
CH3 y
^■X HN^N-CH3 HC ^ rY
VNH ^ YS
H3C CH3 11 12 _>k^„u
CH3 V T CH3
CH3 rJN NH
CH3 || N CH3
CH3 r==\ ^_^\^-rH CH3 r=ur ' L N-CH3 V T UM3 H r X X ,N-CH3
N NH N NH
V^CH3 ^T^N^CH,
F 14 15 V ^
7. A composition comprising crystalline Form I of triaminopyrimidine compound 1 as
claimed in claim 1 or crystalline Form II of triaminopyrimidine compound 1 as claimed in
claim 3, having a purity of 98% or more by weight, and (S)-N2-(4-cyclopropyl-5-fluoro-6-
methylpyridin-2-yl)-N4-(1,5-dimethyl-1H-pyrazol-3-yl)-5-(3,4-dimethyl-piperazine-1-

yl)pyrimidine-2,4-diamine (relative to triaminopyrimidine compound 1) present in an amount of 0.5% or less, by weight by area percentage of HPLC.
8. A composition comprising crystalline Form I of triaminopyrimidine compound 1 as claimed in claim 1 or crystalline Form II of triaminopyrimidine compound 1 as claimed in claim 3, having a purity of 98% or more by weight, and (R)-4-cyclopropyl-6-((4-((1,5-dimethyl-1H-pyrazol-3-yl)amino)-5-(3,4-dimethylpiperazin-1-yl)pyrimidin-2-yl)amino)-3-fluoro-2-methylpyridine-1 -oxide (relative to triaminopyrimidine compound 1) present in an amount of 0.5% or less, by weight by area percentage of HPLC.
9. A process for preparing a crystalline free base compound 2,
CH3
CH3 M
HN""^ HN N
N NH
(I N
\/^\ CH3
V F
2
the process comprising crystallizing the compound 2 from solvent selected from methanol, ethanol, isopropanol, butanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, isopropyl acetate, butyl acetate, dimethylformamide, dimethylacetamide, dimethylsulfoxide, acetonitrile, water, or mixtures thereof; and obtaining the crystalline free base compound 2.
10. A process for the preparation of crystalline Form I of triaminopyrimidine compound
1, comprising crystallizing the triaminopyrimidine compound 1 in acetonitrile, wherein the
crystalline Form I of triaminopyrimidine compound 1 is as defined in claim 1.
11. A process for the preparation of crystalline Form II of triaminopyrimidine compound 1,
the process comprising crystallizing the compound 1 in a mixture of acetonitrile and water,
wherein the crystalline Form II of triaminopyrimidine compound is as defined in claim 3.
12. A pharmaceutical composition comprising crystalline triaminopyrimidine compound 1, and one or more pharmaceutically acceptable carriers, diluents and excipients, wherein the crystalline triaminopyrimidine compound is a crystalline Form I of triaminopyrimidine compound 1 as defined in claim 1, or a crystalline Form II of triaminopyrimidine compound 1 as defined in claim 3.
13. A pharmaceutical composition comprising crystalline triaminopyrimidine compound 1 having a purity of 98% or more by area percentage by HPLC, and one or more
44

pharmaceutically acceptable carriers, diluents, and excipients, wherein the crystalline triaminopyrimidine compound 1 is a crystalline Form I of triaminopyrimidine compound 1 as defined in claim 1, or a crystalline Form II of triaminopyrimidine compound 1 as defined in claim 3.
14. A compound selected from the compound 11, compound 12, compound 13, compound 14 and compound 15,
CH3 y
^N^3 HN-V^3 HC ^ rx
N NH I I
I kN^NH
A N X
M n
H3C CH3 11 12 _X<^Knu
CH3 V T CH3
CH3 p=N NH
CH3 f| T0 F"3
CH3 r==\ ^Av^rH CH3 fA
H r X X> .N~CH3 V T °h3 H,c I L ,N-CH3
H3CS-A HN-^N' 3 V F H3(S^| HN-^N
^NA 13 ^NY^N
II J II
N NH N NH
T CH3 « XfTCH*
l 14 15 V ^