A Process Of Manufacturing Phosphatidyl Serine 90% From Crude Liquid Lecithin

Abstract

This invention relates to a process of extraction of Phosphatidyl Serine-90% from crude Liquid Lecithin, more particularly the extraction of Phosphatidyl Serine rich fraction from crude Lecithin which is derived from vegetable oil sources like Soyabean, Cotton seed, Rapseed, Corn, Rice Bran, Sunflower seeds, Peanuts etc. Soyabean is a natural crop having multipurpose uses for human beings.

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FORM 2
THE PATENTS ACT, 1970
COMPLETE SPECIFICATION (SECTION 10)
THE PROCESS OF MANUFACTURE OF PHOSPHATIDYL SERINE 90 % FROM CRUDE LIQUID LECITHIN
SONIC BIOCHEM EXTRACTIONS LTD., A Limited Company registered under the Companies Act, 1956, having its office at 38, Patel Nagar, Indore, Madhya Pradesh, India, PIN 452 001.
The following specifications particularly describe the nature of this invention and the manner in which it is to be performed:-
Characteristic: Deoiled, wax - Paste like, Solid, white to off white.
Composition : Mixture of Phospho & glycolipids
Phospholipid Complex : 97 % Minimum
Phosphatidyl Serine : Minimum 90 %
Acid Value : NMT 10
Moisture : NMT 1.5 %
Peroxide Value : NMT 5
Solubility : Soluble in Chloroform, Toluene, Insoluble in Ethanol, Methanol, Water
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This invention relates to a process of extraction of Phosphatidyl Serine - 90% from crude Liquid Lecithin, more particularly the extraction of Phosphatidyl Serine rich fraction from Crude Lecithin which is derived from vegetable oil sources like Soyabean, Cotton seed, Rapseed, Corn, Rice Bran, Sunflower seeds, Peanuts etc. Soyabean is a natural crop having multipurpose uses for human beings. It had its origin in China and was developed in India around 1965. Soyabean was primarily developed and grown in India for its high oil content and its uses in improving nitrogen content of land. The crops had a fast growth and the major portion is grown in M.P. accounting for 70% of India's production and rest developed in Rajasthan and Vidharbh area of Maharashtra. The size of the crop is around 70 lacs tons per year, which yields around 13 lacs tons of Soyabean oil.
Soyabean is a natural boon to humanity, it contains oil, proteins, phospholipids, Vitamin E (Tocopherol) and poly unsaturated fatty acids. Till now conventionally in India, Soyabeans are being used mainly for oil extraction and deoiled cake production. Gums and sludge produced during oil refining are not used for any value addition although these contain valuable fractions like Lecithin, Phospholipids.
Our R&D unit has developed the process and the technology of extraction of Phosphatidyl Serine - 90 % from crude liquid lecithin derived for Soyabean oil gums.
Lecithin is a group of naturally occurring phospholipids that's found in nearly every living cell. Though the word lecithin is derived from the Greek term lekithos meaning "yolk of an egg", the primary commercial source of lecithin is Soyabean.
Lecithin is widely used in manufactured food, feed and pharmaceutical, cosmetic and industrial applications such as crystal formation and antidusting, modifying, emulsilying, dispersing, wetting, penetrating & antioxidising properties.
Lecithin is a naturally occurring group of phospholipids, which are important constituents of cell membrane and polyunsaturated fatty acids.
The typical composition of phospholipids and fatty acids in lecithin is as under: -
A) Phospholipids:
1) Phosphatidyl Choline (PC) -23%
2) Phosphatidyl Ethanolamine (PE) - 20%
3) Phosphatidyl Inositol (PI) - 14%
4) Phosphotidic Acid (PA) - 5%
5) Phosphatidyl Serine (PS) - 0.2%
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B)

Fatty Acid in Soyabean Lecithin:
1) Myristic Acid - 1.5%
2) Palmitic Acid - 18.9%
3) Palmito Oleic Acid - 8.6%
4) Stearic Acid - 3.9%
5) Oleic Acid - 9.8%
6) LinoleicAcid - 20%
7) Linolenic Acid - 2.5%
8) Arachidic Acid - 2.1%

The constitutional formula of Phosphatidyl Serine is as under:

Where:
R = Fatty Acid
R1 = O- P - OR2 - or Fatty Acid
OH
R2 = CH2-CH2-N (CH3)3 - Phosphatidyl Choline
= GH2-CH2-NH3 - Phosphatidyl Ethanolamine
= CH2-CH (NH3)-COOH - Phosphatidyl Serine
= C6 H6(OH)6 - Phosphatidyl Inositol
= H - Phosphatidic Acid
Lecithin also contains natural antioxidant as Tocopherol and hence acts as a good antioxidant particularly because of its ability to complex heavy metals.
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WE CLAIM
A process for producing Phosphatidyl Serine - 90 % from crude liquid lecithin comprising following steps:
1) Etheral solution of purified liquid lecithin from soyabeans is mixed with Serine solution in the ratio of 1 : 0.05
2) It is then treated with enzymes from phospholipase - D ( PLD ) series in the ratio of 1 : 10.
3) Reaction is carried out with stirring for 30 minutes at room temp.
4) After 5-10 minutes, 1 M HCI is then added to the mixture in the ratio of 1 : 10
5) Whole lot is then centrifuged.
6) Top etheral layer so obtained contains Phosphatidyl Serine & Phosphatidyl Inositol. It is then dried to obtain Powder.
7) Bottom layer is separated out, contains water, residue and enzyme.
8) Ether is added to the powder containing Phosphatidyl Serine & Phosphatidyl Inositol. Phosphatidyl Serine is dissolved in ether. Phosphatidyl Inositol is precipitated as bottom layer and settled down.
9) Etheral phase (top layer) containing Phosphatidyl Serine and ether is then distilled under vacuum.

10) Phosphatidyl Serine is obtained as Dry powder.
11) Final product is then tested by HPLC to assess the-purity^

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