FORM-2
THE PATENTS ACT, 1970
(39 OF 1970)
AND
THE PATENTS RULES, 2003
(As Amended)
COMPLETE SPECIFICATION
(See section 10; rule 13)
"A QUANTITATIVE ASSAY FOR 4-PYRROLIDINOPYRIDINE (4-PPY) IN POLYSACCHARIDE-PROTEIN
CONJUGATE VACCINES"
SERUM INSTITUTE OF INDIA LTD., a corporation organized and existing under the laws of India, of 212/2, Off Soli Poonawalla Road, Hadapsar, Pune 411 028, Maharashtra, India
The following specification particularly describes the nature of this invention and the manner in which it is to be performed:

Brief Description:
Vaccines that contain protein covalently linked to carbohydrate have proven remarkably successful in inducing an immune response to the carbohydrate moiety. Examples of such vaccines, known as "conjugates" are available for Haemophilus influenzae type b (e.g., ActHib, Hiberix) , Neisseria meningitidis types AC W and Y (e.g., Menactra) andS. pneumoniae (e.g., Prevnar, Synflorix) . In order for the protein to be linked to the carbohydrate, the latter generally needs to be activated so that it can be reacted with the protein, either directly or via a spacer (Dick, W.E. Jr and Beurret, M. Glyco-conjugates of bacterial carbohydrate antigens. A survey and consideration of design and preparation factors. In: Conjugate Vaccines (Eds Cruse, J.M. and Lewis, R.E. ) .
Improved methods for cyanating polysaccharides use 1 -cyano-4-dimethylaminopyridine tetrafluoroborate (CDAP) (Lees, A., Producing immunogenic constructs using soluble carbohydrates activated via organic cyanylating reagents. U.S. Patent Nos. 5,651,971; 5,693,326; and 5,849,301). GSK' s Synflorix is an approved multivalent pneumococcal polysaccharide-protein conjugate vaccine prepared by using CDAP based cyanylation conjugation chemistry.Further GSK's Nimenrix is a pipeline multivalent meningococcal polysaccharide-protein conjugate vaccine prepared by using CDAP based cyanylation conjugation chemistry. The activation of the polysaccharide with CDAP introduces a cyanate group in the polysaccharide and DMAP(4-dimethylamino-pyridin) is liberated. It is known that residual DMAP results in degradation of polysaccharide .Further DMAP has a rat LD50 (oral) of 140 mg/kg .To address above concerns of DMAP and subsequent queries raised by regulatory agencies , GSK has previously disclosed in-house assays to quantify residual DMAP content in CDAP conjugation chemistry based polysaccharide-protein conjugate vaccines (Synflorix, Nimenrix)as well as utilized diafiltration for removal of DMAP.Refer WO00/56359 and WO2007000342 .
Regulatory authorities do consider potential toxicity of the identified exogenous process related impurities and accordingly

require that the same should be considerably lower than the 1.5 ug/dose .Refer EMEA Guideline(EMEA /CHMP /QWP/251344/2006) on 'The Limits of Genotoxic Impurities'.
It has been reported previously that the polysaccharide-protein conjugate purification procedures should remove residual reagents used for conjugation and the removal of reagents and reaction by-products such as cyanide, EDAC and others depending on the conjugation chemistry, should be confirmed by suitable tests or by validation of the purification process wherein the acceptable limits should be approved by the NRA. Refer Section A.3.3.1 WHO/BS/06.2041 2006 "Recommendations to assure the quality, safety and efficacy of group A meningococcal conjugate vaccines"
Polysaccharide- protein conjugate vaccines prepared using l-Cyano-4- pyrrolidinopyridinium tetrafluorborate (CPPT)based cyanylation conjugation chemistry are still in development phase and yet to be approved by regulatory agencies.Inventors have surprisingly found that during conjugation process l-Cyano-4- pyrrolidinopyridinium tetrafluorborate gets hydrolysed to form a reaction by-product"4-Pyrrolidinopyridine (4-PPY)".
Further 4-PPY has a rat LD50 (oral) of 176 mg/kg.To address above concerns of 4-PPY and queries that could be raised by regulatory agencies , there is an urgent need to develop an assay to quantify residual 4-PPY content in CPPT conjugation chemistry based polysaccharide-protein conjugate vaccines.Particularly, quantification of 4-PPY would be necessary for pipeline multivalent meningococcal polysaccharide protein conjugate vaccine compositions comprising capsular saccharide from serogroups X and capsular saccharide from A, C, W135 and Y prepared by using l-Cyano-4-pyrrolidinopyridinium tetraf luorborate based cyanylation conjugation chemistry . Refer WO2013/114268.
The 4-PPY formed as a by-product of CPPT cyanylation conjugation chemistry during preparation of polysaccharide- protein

conjugates has basic ring of pyrrolidinopyridine which has non-polar characteristics. In a bulk conjugate proteins have relatively less aqueous solubility than polysaccharide molecules. The polysaccharide protein conjugates have high molecular weight (above 900KDa) . The molecules of high molecular weight are known to further reduce solubility. This physical attribute coupled with low temperature makes the polysaccharide-protein conjugate molecules in aqueous to orient in micellar form wherein each conjugate bulk is stored at or above 0.3 mg/ ml concentration. Particularly,the location of 4-PPY as residual in micellar polysaccharide-protein conjugate solution is thought to be in the protein core of micelle. Such entrapment of 4PP in micellar form causes great difficulty in releasing 4-PPY at normal temperature when estimating 4-PPY for in-process samples.
Gas chromatography (GC) based method for 4-PPY purity assessment in pure 4-PPY sample is known .Refer WeylChem GmbH, Mannheim fine chemicals/APIs booklet.Use of GC method for quantification of 4-PPY in polysaccharide-protein conjugate vaccine or bulk polysaccharide-protein conjugate has not been reported.However it is known that GC method is not suitable for in-process analysis where immediate estimation of residuals is required for following reasons i) requires complex sample preparation ii) long analysis time , iii) additional time for column stabilization and iv) is imprecise due to possibility of interference of sample matrix and appearance of ghost peaks.
None of the prior art methods are applicable to 4-PPY quantification in polysaccharide-protein conjugate vaccine and polysaccharide protein conjugate bulk.
The present invention arises from the surprising finding that it is possible to quantify 4-PPY in polysaccharide- protein conjugate vaccine and polysaccharide-protein conjugate bulk by utilizing a novel REVERSED Phase - HPLC based assay that comprises of heating sample containing polysaccharide protein conjugate at a temperature between 40 °C and 90°C for a duration between lmin and 3hrs followed by RP-HPLC.Sample heating step

of instant method is postulated to increase the diffusion of 4-PPY into non-micellar regions thereby resulting in collapse of micelles of polysaccharide-protein conjugates.Further said higher temperatures are also expected to increase the solubility of 4-PPY and release ionically associated 4-PPY in aqueous bulk. Heating step was found to provide correct concentration of 4-PPY whereas estimation of 4-PPY without heating was found to provide 4 to 5 times less 4-PPY concentration.
Present invention eliminates the foregoing shortcomings of the prior art methods of assaying for 4-PPY in polysaccharide-protein conjugate vaccine and polysaccharide-protein conjugate bulk by providing a rapid and reproducible (RP-HPLC) based method for quantifying residual 4-pyrrolidinopyridine(4-PPY) in polysaccharide- protein conjugate vaccines prepared by cyanylation conjugation chemistry using l-Cyano-4- pyrrolidinopyridinium tetrafluorborate (CPPT) .
Summary of Invention:
The invention relates to a novel, rapid and reproducible (RP-HPLC) method for quantifying residual 4-pyrrolidinopyridine(4-PPY) in polysaccharide- protein conjugate vaccines prepared by cyanylation conjugation chemistry using l-Cyano-4- pyrrolidinopyridinium tetrafluorborate (CPPT).
Instant assay for 4PPY quantification in polysaccharide-protein
conjugate samples provides following advantages:
l.Said method is applicable for quantification of 4-PPY in any
polysaccharide- protein conjugates(having capsular
polysaccharides from Streptococcus Pneumoniae, Neisseria
meningitidis , Haemophilus influenzae)prepared by using CPPT
conjugation chemistry.
2. It can be utilized for 4PPY quantification for in-process(bulk
polysaccharide-protein conjugate) as well as final
polysaccharide-protein conjugate vaccines.
3.It has a limit of detection for 4-PPY of about 5ng/ml.

4.It is a rapid method with a total duration of 8 minutes.
5.It is reproducible.
6.It does not require any tedious sample preparation.
7.None of the sample matrix interferes with the 4-PPY peak.
Description of the figures
Figure 1: 4-Pyrrolidinopyridine (4-PPY) standard scanned in the
UV range using UV-Visible spectrophotometer shows absorption
maxima at about 280nm.
Figure 2: 4-Pyrrolidinopyridine (4-PPY) standard injected into
the HPLC with Photodiode array (PDA) detector shows absorption
maxima at about 280nm.
Figure 3: Standard Chromatogram of 4-PPY (lppm)
Figure 4: Men W-CRM conjugate injected as such (Initial)
Figure 5: Men W-CRM conjugate heated at 45°C for 3hrs.
Figure 6 :Men W-CRM conjugate heated at 60°C for 3hrs.
Figure 7 :Men W-CRM conjugate heated at 90°C for 3hrs.
Figure 8 :Overlay chromatograms of Men W-CRM conjugate.
Detailed Description
An important aspect of the instant invention is that said 4-PPY
quantification assay comprises of following steps:
1.Preparation of Trifluoroacetic acid having concentration
between 0.01 to 0.1% in water followed by mixing of solution.
2.Preparation of mobile phase containing Trifluoroacetic acid
and Acetonitrile in a ratio selected from 80:20,50:50 and 90:10
having a final pH between 2 and 2.4.
3.Saturation of analytical Reversed-Phase High-Performance
Liquid Chromatography (RP-HPLC) column.
4.Setting column conditions of temperature at about 25o C,flow
rate between 0.8 and 1.2 mL/min,wavelength of 280nm.
5.Injection of known standard solution of 4-PPY(lppm).
6.Subjecting polysaccharide protein conjugate samples
(in-process or final vaccine) to a temperature between 40°C and
90°C for a duration between lmin and 3hrs
7.Injecting preheated sample of about 10 uL

8.estimation of residual 4-PPY.
Thus, in a preferred embodiment, the invention relates to a RP-HPLC based assay for quantification of 4PPY comprising of a pre-heating step wherein heating is performed at a temperature between about 40°C and about 90°C, more preferably between about 45°C and about 90°C, and even more preferably between about 50°C and about 80°C, most preferably between about 60°C and about 90°C.
Another aspect of instant invention is that said heating of polysaccharide-protein conjugate is carried for a duration between about 1 min and 3hrs, more preferably between about lhr and about 2 hr,and even more preferably between about 90 minutes and 3hr, most preferably between 60 minutes and 90 minutes.
An important aspect of the invention is that said superficially porous silica-based reversed-phase support can be derivatized with C4, C8, C18, phenyl or cyano moieties, more preferably reverse phase support can be selected from C-18 USP LI and C-8 USP L7,most preferably C-8 USP L7.
Another aspect of the instant invention is that said C-8 HPLC column can have dimensions selected from 150x4.6mm,250x4.6mm and 300 x4.6mm.
The mobile phase can consist of an organic modifier and an organic acid wherein the organic modifier can be selected from the group consisting of acetonitrile and methanol whereas the organic acid can be selected from the group consisting of trifluoroacetic acid and heptafluorobutyric acid.
A further embodiment of the instant invention is that mobile phase consists of Trifluoroacetic acid and Acetonitrile in a ratio selected from 80:20,50:50 and 90:10,most preferably 80:20.
Preferably,RP-HPLC uses an analytical column with isocratic mode wherein column temperature can be between 20°C and 25°C and

flow rate can be between 0.8 and 1.2 mL/min.
An important embodiment of instant invention is that the 4-PPY content estimated by instant method employing heating step prior to RP-HPLC is about 4 to 15 fold more as compared to the 4-PPY content obtained with direct RP-HPLC.
A second aspect of the instant invention is that said quantification assay for 4-PPY can be utilized for monovalent conjugates or multivalent meningococcal polysaccharide protein conjugate vaccine compositions comprising capsular saccharide from serogroups X and capsular saccharide from A, C, W135 and Y prepared by using l-Cyano-4-pyrrolidinopyridinium tetrafluorborate based cyanylation conjugation chemistry.
Yet another aspect of the invention is that above method for 4PPY quantification can be applicable to all polysaccharide-protein conjugate vaccines wherein said polysaccharide is a bacterial capsular polysaccharide selected from the group consisting of Neisseria meningitidis groups A , C , W135 Y and X, Haemophilus influenzae,Group A Streptococcus, Group B Streptococcus, Streptococcus pneumoniae serotypes 1, 2, 3, 4, 5, 6A, 6B,7F,9A,9V,9N,10A, 11A ,12F,14,15B, 17F, 18C, 19A , 19F, 20, 22F,23F , 33F and 45, Escherichia coli, Francisella tularensis, Klebsiella, Moraxella catarrhalis, Porphyromonas gingivalis, Pseudomonas aeruginosa, Burkholderia cepacia, Salmonella typhi , Salmonella typhimurium, Salmonella paratyphi, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Vibrio cholera, Enterococcus faecalis, Enterococcus faecium, Mycobacterium tuberculosis, Staphylococcus aureus and Staphylococcus epidermidis.
The carrier protein of said polysaccharide-protein conjugates can be selected from a group of but not limited to CRM 197,diphtheria toxoid,tetanus toxoid, fragment C of tetanus toxoid, pertussis toxoid,protein D of H. influenzae, E. coli LT, E: coli ST, and exotoxin A from Pseudomonas aeruginosa, outer membrane complex c (OMPC) , porins, transferrin binding proteins, pneumolysin, pneumococcal surface protein A (PspA) ,

pneumococcal adhesin protein (PsaA),pneumococcal PhtD, pneumococcal surface proteins BVH-3 and BVH-11 , protective antigen (PA) of Bacillus anthracis and detoxified edema factor (EF) and lethal factor (LF) of Bacillus anthracis, ovalbumin, keyhole limpet hemocyanin (KLH), human serum albumin, bovine serum albumin (BSA) and purified protein derivative of tuberculin (PPD),particularly CRM197.
The following examples are intended to illustrate one embodiment now known for practicing the invention, but the invention is not to be considered limited to these examples
Examples
I) Preparation of MenA-TT Conjugate
Polysaccharide preparation:
MenA-TT conjugate was prepared by cyanylation and carbodiimide chemistry. The native polysaccharide was sized reduced by mechanical means. The sized reduced PS (100-120kD on SEC-HPLC) was diafiltered & concentrated on lOkD cuttoff membrane. To 6mg/ml PS, freshly prepared solution of CPPT(dissolved 114 mg/ml in acetonitrile) added the pH was shifted to 9.5 with NaOH solution and hold for upto 5 min. Adipic acid dihydrazide (ADH) dissolved lOOmg/ml in water was added to the PS solution. The reaction was stirred for 1 hrs at 25°C. The reaction was quenched by addition of excess glycine solution. The reaction mixture was diafiltered on lOkD TFF in MES buffer to remove residuals and unreacted components. The sample was stored at 2-8°C until use. The PS content was measured by phosphorous assay and extent of derivatization was measured by TNBS assay.
Carrier Protein (TT) Preparation:
Tetanus toxoid was chromatographically purified to remove aggregates that might cause adverse effect in the conjugation process. Total TT load was 5-10% v/v of packed column (toyopearl HW-65F, Tosoh biosciences) with 30cm/hr linear flow rate. The fractions were collected on the basis of chromatogram obtained on UV detector of the chromatography system. The fractions were

pooled on the basis of aggregate removal seen on SEC-HPLC. The collected fractions were subjected to concentration-diafiltration on 30kD TFF in MES buffer. The protein content was measured by Lowry assay.
Conjugation of Men A to TT:
The ADH-derivatized PS, purified TT and EDAC (l-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide) were mixed the ratio of 1:0.8:1.5 by weight under stirring condition. The reaction was monitored on HPLC for protein conversion. The reaction was quenched after 3 hours by raising pH with lOmM PB+EDTA pH 8.0 buffer.
Conjugate Purification:
The conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOOmM PBS and followed by 30 volumelOmM Tris pH 7.2. The sucrose-citrate stabilizer was added to bulk conjugate and stored below -20°C until use. The purified bulk conjugate was analyzed for total and unbound (free) PS, Total and unbound(free) protein and residual 4-PPY in the conjugate bulk.
Table 1

Test Sample Total PS mg/ml Total
Protein
mg/ml % Free PS % Free Protein
Men A-TT
Purified
Conjugate 1.1 2.23 4.10 2.27
II) Preparation of MenC-CRM197 Conjugate
Polysaccharide preparation:
MenC PS was size-reduced by high pressure homogenization to 75-100kDa on SEC -HPLC, which is 40-50kD on MALLS and the PS was then diafiltered and concentrated on lOkD cuttoff membrane

Carrier Protein (CRM197) Preparation:
In order to conjugate Men C to CRM197, the carrier protein was derivatized by ADH linker prior to conjugation. The native CRM197 was allowed to react with ADH linker in presence of EDAC (l-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide) in MES buffer. The reaction was then quenched by increasing pH by sodium carbonate solution under stirring for 15min. The ADH-derivatized CRM197 was then diafiltered on lOkD cuttoff membrane using lOmM carbonate buffer pH 9.5 by passing about 35 volumes of buffer. The sample was submitted for content analysis and extent of derivatization.
Conjugation of Men C to CRM197:
MenC PS was mixed with CPPT( in acetonitrile) and then with ADH-derivatized CRM197 in the ratio of 1:0.7:1.25 by weight under stirring condition. The reaction was monitored on HPLC. The reaction was quenched after 4 hours by addition of excess glycine solution.
Conjugate Purification:
The crude conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOOmM PBS and followed by 10 volume lOmM Tris pH 7.2 and then 20 volumes of lOmM Tris on lOOkD cut off membrane. The sucrose-citrate stabilizer mixture was added to the bulk conjugate and stored below -20°C until use. The purified bulk conjugate was analyzed for total and unbound (free) PS, Total and unbound(free) protein and residual 4-PPY in the conjugate bulk.
Table 2

Test Sample Total PS mg/ml Total
Protein
mg/ml % Free PS % Free Protein
Men C-CRM197
Purified
Conjugate 1.10 2.11 <2.16 4.5

Ill) Preparation of MenY-CRM197 Conjugate
Polysaccharide preparation:
Men Y PS sizing was carried out by chemical means . The chemically sized PS was targeted to achieve size of 75-100kDa on SEC-HPLC and then the polysaccharide was diafiltered on lOkD cuttoff membrane.
Carrier Protein (CRM197) Preparation:
The carrier Protein derivatization process was same as described above for MenC-CRM conjugate.
Conjugation of Men Y to CRM197:
MenY PS was mixed with CPPT (113 mg/ml in acetonitrile) , pH adjusted to 9.5 immediately after addition and then with ADH-derivatized CRM197 in the ratio of 1:1.5:1.25 by weight under stirring condition. The reaction was monitored on HPLC. The reaction was quenched by addition of excess glycine solution.
Conjugate Purification:
The conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOOmM PBS and followed by 10 volumelOmM Tris pH 7.2 and then 20 volumes of lOmM Tris on lOOkD cut off membrane. The sucrose-citrate stabilizer mixture was added to the bulk conjugate and stored below -20°C until use.
The purified bulk conjugate was analyzed for total and unbound (free) PS, Total and unbound(free) protein and residual 4-PPY in the conjugate bulk.
Table 3

Test Sample Total PS mg/ml Total
Protein
mg/ml % Free PS % Free Protein
Men Y-CRM197
Purified
Conj ugate 1.46 3.59 <4.43 <4.09

IV) Preparation of MenW-CRMl97 Conjugate
Polysaccharide preparation:
In case of Men W PS, sizing was carried out by mechanical means and sizing continued until the PS size was reached to 120-130 kD on SEC-HPLC and then the polysaccharide was diafiltered-concentrated on lOkD cuttoff membrane.
Carrier Protein (CRM197) Preparation:
The carrier Protein derivatization process was same as described above for MenY-CRM197 conjugate.
Conjugation of Men W to CRM197:
MenW PS was mixed with CPPT (113 mg/ml in acetonitrile) , pH adjusted to 9.5 immediately after addition and then ADH-derivatized CRM197 was added in the ratio of 1:0.8:0.8 by weight under stirring condition. The reaction was monitored on HPLC for protein peak to disappear. The reaction was quenched after 4 hours by addition of glycine buffer.
Conjugate Purification:
The conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOOmM PBS and followed by 10 volumelOmM Tris pH 7.2 and then 20volumes of lOmM Tris on lOOkD cut off membrane. The sucrose-citrate stabilizer mixture added to the bulk conjugate and stored below -20°C until use.The purified bulk conjugate was analyzed for total and unbound (free) PS, Total and unbound(free) protein and residual 4-PPY in the conjugate bulk.
Table 4

Total
Total PS Q, Free % Free
Test Sample Protein
mg/ml mg/ml PS Protein

Men W-CRM197 1.53 2.54 <4.25 <4.08
Purified
Conjugate
V) Preparation of MenX-TT Conjugate
Polysaccharide preparation:
MenX-TT conjugate was prepared by cyanylation and carbodiimide chemistry. The native polysaccharide was size- reduced by mechanical means. The size-reduced PS (120-150kD on SEC-HPLC) was diafiltered & concentrated on lOkD cuttoff membrane. To 20 mg/ml PS, freshly prepared solution of CPPT (also known as CPPT dissolved 114 mg/ml in acetonitrile) was added and the pH was shifted to 9.5 with NaOH solution and incubated for 2-4 min. Adipic acid dihydrazide (ADH) was dissolved lOOmg/ml in water and added to the PS solution. The reaction was stirred for 3 hrs at 25°C. The reaction was quenched by addition of excess glycine solution. The reaction mixture was diafilterted in MES buffer to remove residuals and unreacted components. The sample was stored at 2-8°C until use. The PS content was measured by phosphorous assay and extent of derivatization was measured by TNBS assay.
Carrier Protein (TT) Preparation:
The carrier Protein preparation process was similar as described above.
Conjugation of Men X to TT:
The ADH-derivatized PS, purified TT and EDAC (l-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide) were mixed in a ratio of 1:1.5:0.7 by weight under stirring condition. The reaction progress was monitored on HPLC. The reaction was quenched after 3hrs by raising pH with lOmM PB+EDTA pH 8.0 buffer.
Conjugate Purification:
The conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOOmM PBS and followed by 30 volumelOmM Tris pH

7.2. The purified bulk conjugate was analyzed for total and unbound (free) PS, total and protein and residual 4-PPY in the conjugate bulk.
Table 5

Test Sample Total PS mg/ml Total Protein mg/ml % Free PS
Men X-TT Purified Conjugate 1.08 2.44 7.87
VI) Preparation of Pneumococcal 23F-CRM197 Conjugate
Polysaccharide preparation:
In case of Pneumococcal 23F Ps, sizing was carried out by mechanical means and sizing was continued until the PS size reached to 150-170 kD on SEC-HPLC and then the polysaccharide was diafiltered-concentrated on lOkD cuttoff membrane.
Carrier Protein-CRM197 Preparation:
CRM197 protein content 31mg/ml was used for conjugation.
Conjugation of Pneumococcal 23F-CRM197
Pneumococcal 23F PS was subjected mixed with CPPT( in acetonitrile) and then native CRM197 in the ratio of 1:1.25:1.5 by weight under stirring condition. The reaction was monitored on HPLC. The reaction was quenched after 2 hrs by addition of excess glycine solution.
Conjugate Purification:
The conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOmM PBS and followed by 20 volumelOmM Tris pH 7.2 . The purified bulk conjugate was analyzed for total and unbound

(free) PS, total and unbound(free) protein and residual 4-PPY in the conjugate bulk.
Table 6

Test Sample Total PS mg/ml Total
Protein
mg/ml % Free PS % Free Protein
Pneumococcal
23F-CRM197
Conjugate 0.94 1.91 7.43 1.79
VII) Preparation of Pneumococcal conjugate 23F-CRM197-ADH Conjugate
Polysaccharide preparation:
In case of Pneumococcal 23F Ps, sizing was carried out by mechanical means and sizing was continued until the PS size was reached to 150-170 kD on SEC-HPLC and then the polysaccharide was diafiltered-concentrated on lOkD cuttoff membrane.
Carrier Protein (CRM197) Preparation:
In order to conjugate Pneumococcal 23F Ps to CRM197, the carrier protein was derivatized by ADH linker prior to conjugation. The native CRM197 was allowed to react with ADH linker in presence of EDAC (l-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide) in MES buffer. The reaction was then quenched by increasing pH by sodium carbonate solution under stirring for 15min. The ADH-derivatized CRM197 was then diafiltered on lOkD cuttoff membrane using lOmM carbonate buffer pH 9.5 by passing about 35 volumes of buffer. The sample was submitted to content analysis and extent of derivatization.
Conjugation of Pneumococcal 23F-CRM197:
Pneumococcal 23F PS was mixed with CPPT ( in acetonitrile) and then ADH-derivatized CRM197 in the ratio of 1:1.25:1.25 by weight under stirring condition. The reaction was monitored on HPLC. The reaction was quenched after 3 hours by addition of

excess glycine solution.
Conjugate Purification:
The crude conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOmM PBS and followed by 20 volumelOmM Tris pH 7.2. The purified bulk conjugate was analyzed for total and unbound (free) PS, total and unbound(free) protein and residual 4-PPY in the conjugate bulk.
Table 7

Test Sample Total PS mg/ml Total
Protein
mg/ml % Free PS % Free Protein
Pneumococcal
23F-CRM197
Conjugate 0.87 1.58 1.67 2.11
VIII) Preparation of Pneumococcal 9V-TT Conjugate
Polysaccharide preparation:
Pneumococcal 9V PS, sizing was carried out by mechanical means and sizing continued until the PS size was reached to 150-170 kD on SEC-HPLC and then the polysaccharide was diafiltered-concentrated on lOkD cuttoff membrane.
Carrier Protein (TT) Preparation:
Tetanus toxoid was chromatographically purified to remove aggregates that might cause adverse effect in the conjugation process. Total TT load was 5-10% v/v of packed column (toyopearl HW-65F, Tosoh biosciences) with 30cm/hr linear flow rate. The fractions were collected on the basis of chromatogram obtained on UV detector of the chromatography system. The fractions were pooled on the basis of aggregate removal seen on SEC-HPLC. The collected fractions subjected to concentration-diafiltration on 30kD TFF in MES buffer. The protein content was measured by Lowry assay. Conjugation of Pneumococcal 9V to TT:

Pneumococcal 9V PS was mixed with CPPT (in acetonitrile) and then TT in the ratio of 1:0.7:1.5 by weight under stirring condition at pH 9-10. The reaction progress was monitored on HPLC. The reaction was quenched after lhr 30 min by addition of excess glycine solution.
Conjugate Purification:
The crude conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOmM PBS and followed by 20 volume 10mM Tris pH 7.2. The purified bulk conjugate was analyzed for total and unbound (free) PS, Total and unbound (free) protein and residual 4-PPY in the conjugate bulk.
Table 8

Test Sample Total PS mg/ml Total Protein mg/ml % Free PS
Pneumococcal 9V-TT Conjugate 0.80 1.44 <2
IX) Preparation of Pneumococcal 9V-CRM197 Conjugate
Polysaccharide preparation:
Pneumococcal 9V PS, sizing was carried out by mechanical means and sizing continued until the PS size was reached to 150-170 kD on SEC-HPLC and then the polysaccharide was diafiltered-concentrated on lOkD cuttoff membrane.
Carrier Protein (CRM197) Preparation:
CRM197 protein content 31mg/ml was used for conjugation.
Conjugation of Pneumococcal 9V to CRM197:
Pneumococcal 9V PS was mixed with CPPT ( in acetonitrile) and then CRM197 in the ratio of 1:1:1.5 by weight under stirring condition. The reaction was monitored on HPLC. The reaction was quenched after 2 hours by addition of excess glycine solution.
Conjugate Purification:

The conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOmM PBS and followed by 10 volumelOmM Tris pH 7 . 2 The purified bulk conjugate was analyzed for total and unbound (free) PS, total and unbound (free) protein and residual 4-PPY in the conjugate bulk.
Table 9

Test Sample Total PS mg/ml Total
Protein
mg/ml PS Free % Free Protein
Pneumococcal
9V-CRM197
Conjugate 1.01 1.98 <2 2.64
X) Preparation of Pneumococcal 14-CRM197-ADH Conjugate
Polysaccharide preparation:
In case of Pneumococcal 14 Ps, sizing was carried out by mechanical means and sizing was continued until the PS size reached to 100-120 kD on SEC-HPLC and then the polysaccharide was diafiltered-concentrated on lOkD cuttoff membrane.
Carrier Protein (CRM197) Preparation:
In order to conjugate Pneumococcal 14 Ps to CRM197, the carrier protein was derivatized by ADH linker prior to conjugation.
The native CRM197 was allowed to react with ADH linker in presence of EDAC (l-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide) in MES buffer. The reaction was then quenched by increasing pH by sodium carbonate solution under stirring for 15min. The ADH-derivatized CRM197 was then diafiltered on lOkD cuttoff membrane using lOmM carbonate buffer pH 9.5 by passing about 35 volumes of buffer. The sample was submitted to content analysis and extent of derivatization.
Conjugation of Pneumococcal 14-CRM197:

Pneumococcal 14 PS was mixed with CPPT ( in acetonitrile) and then ADH-derivatized CRM197 in the ratio of 1:1.25:1.25 by weight under stirring condition. The reaction progress was monitored on HPLC. The reaction was quenched after 3 hours by addition of excess glycine solution.
Conjugate Purification:
The crude conjugate was purified on 300kD cut off membrane by passing 30 volumes of lOmM PBS and followed by 20 volumelOmM Tris pH 7.2. The purified bulk conjugate was analyzed for total and unbound (free) PS, total and unbound(free) protein and residual 4-PPY in the conjugate bulk.
Table 10

Test Sample Total PS mg/ml Total
Protein
mg/ml % Free PS % Free Protein
Pneumococcal
14-CRM197
Conjugate 1.22 1.43 5.29 <1
Analysis of Meningococcal conjugate for 4-PPY content:
Meningococcal conjugate (s) (A C W Y X) were analysed for the
4-PPY content. 4-PPY quantification was carried out as follows:
1.Trifluoroacetic acid (0.05%) was prepared in water followed
by mixing of solution.
2 .Mobile phase containing Trif luoroacetic acid and Acetonitrile
in a ratio 80:20 having a final pH between 2 and 2.4 was prepared.
3.Analytical Reversed-Phase High-Performance Liquid
Chromatography (RP-HPLC) column was saturated.
4 .Column temperature was set at about 25° C, flow rate was between
0.8 and 1.2 mL/min.
5.Known standard solution of 4-PPY(lppm)was injected.
6.Polysaccharide protein conjugate samples (in-process or final

vaccine)were subjected to 90°C for a duration between of 3hrs .
7.preheated sample 10 uL was injected.
8.4-PPY was estimated at a wavelength of 280nm.
Table 11- 4-PPY content in Meningococcal conjugate(s) (A C W Y X)

Samples 4- PP (ng/mL)

Initial Samples
heated at
45°C for
3hrs . Samples heated
at 60°C for
3hrs . Samples
heated at
90°C for
3hrs .
Men A-
TT Conjugate 8 19 23 27
MEN C-
CRM Conjugate 126 352 384 654
MEN Y-
CRM Conjugate 523 1067 1135 1994
MEN W-
CRM Conjugate 175 396 422 694
MEN X-
TT Conjugate 6 29 31 51
It was observed that when the sample was exposed to elevated temperatures i.e. 45°C, 60°C, 90°C for about 3hrs 4-PP content ted was found to be 3 to 4 times more at 90°C as compared to the 4-PPY content obtained without heating.
Analysis of Pneumococcal conjugate(s) for 4-PPY content:
Pneumococcal conjugate (s) (9V,14,23) were analysed for the 4-PP content.
Table 12- 4-PPY content in Pneumococcal conjugate (s) (9V,14,23F)

Samples
4-PPY (ng/mL)

Initial Samples
heated at
45°C for
3hrs. Samples
heated at
60°C for
3hrs . Samp1es
heated at 90°C
for 3hrs.
PN9V- 139 274 587 2458
CRM
Conjugate
PN9V- 188 429 848 4425
TT Conjugate
PN14- 113 206 446 1585
CRM
Conjugate
PN23F- 512 545 1435 3608
CRM
Conjugate-1
PN23F- 378 669 1075 2664
CRM
Conjugate-2
It was observed that when sample(s) were exposed to elevated temperature i.e. 45°C, 60°C, 90°C for about 3hrs the estimated 4-PPY content was found to be 10 to 15 times more at 90°C as compared to that obtained for sample(s) without heating .
It is to be understood that typical methods of RP-HPLC technology have been applied, and that a person skilled in the art of RP-HPLC and HPLC is well aware of minor adjustments that would not alter the results to be obtained, such as different measurements at other suitable wavelengths or by the use of other column material that would not severely alter the results obtained by the invention.

In view of the many possible embodiments to which the principles of the disclosed invention may be applied, it should be recognized that the illustrated embodiments are only preferred examples of the invention and should not be taken as limiting the scope of the invention. Rather, the scope of the invention is defined by the following claims. We therefore claim as our invention all that comes within the scope and spirit of these claims.

We Claim:
1. An improved method for assaying a sample suspected to contain
4-pyrrolidinopyridine(4-PPY)comprising the steps of:
(i)incubating sample containing 4-PPY at a temperature between 40° C and 90° C for a duration between lhr and 3hrs. (ii) subjecting pre-heated sample to a reversed phase high performance liquid chromatography. (iii) detecting any 4-pyrrolidinopyridine (4-PPY) .
2. A method according to claim 1, wherein the sample is a
polysaccharide- protein conjugate prepared by cyanylation
conjugation chemistry using l-Cyano-4- pyrrolidinopyridinium
tetrafluorborate (CPPT).
3. A method according to claim 1, wherein the heating in step (i) is performed at a temperature between 60° C and 90° C for a duration between 60 minutes and 180 minutes.
4. A method according to claim 3, wherein the heating in step (i) is performed at 90° C for a duration of 180 minutes.
5 . A method according to claim 1, wherein the reversed phase HPLC uses an Isocratic mode.
6. A method according to claim 1 , wherein the superficially porous silica-based reversed-phase analytical column support is selected from a group consisting of C4, C8, C18, phenyl and cyano.
7. A method according to claim 6 ,wherein the analytical column is C8 (as USP L7) .
8. A method of claim 1,wherein mobile phase for reversed phase HPLC consists of an organic modifier and an organic acid.
9. A method of claim 8, wherein the organic modifier is selected from the group consisting of acetonitrile and methanol.

10. A method of claim 8, wherein the organic acid is selected from the group consisting of trifluoroacetic acid and heptafluorobutyric acid.
11. A method of claim 8 , wherein mobile phase consists of trifluoroacetic acid and acetonitrile in a ratio selected from 80:20,50:50 and 90:10.
12. A method of claim 11, wherein mobile phase consists of Trifluoroacetic acid and Acetonitrile in a ratio of 80:20.
13. A method according to claim 12 , wherein mobile phase comprises between 0.01 and 0.1% by volume of trifluoroacetic acid.
14. A method according to claim 13, wherein mobile phase comprises between 0.05% by volume of trifluoroacetic acid.
15. A method according to claim 1 , wherein the mobile phase of
reversed phase-HPLC has a pH between 2 and 2.5.
16. A method according to claim 1 ,wherein the reversed phase-HPLC column temperature can be between 20°C and 25°C.
17. A method according to claim 16 ,wherein the column temperature is 25°C.

18. A method of claim 1, wherein the flow rate for reversed phase-HPLC can be between 0.8 and 1.2 mL/min.
19. A method of claim 1,wherein the detection is carried at a wavelength of 280nm.
20. A method according to claim 1, wherein the 4-PPY content estimated by subjecting sample to heating prior to RP-HPLC is atleast 4 fold more as compared to the 4-PPY content obtained with direct RP-HPLC.
21. The method of any preceding claim wherein the capsular

polysaccharide is a polysaccharide selected from a Meningococcal polysaccharide, a Pneumococcal polysaccharide, a Haemophilus influenzae type B polysaccharide, a Vi polysaccharide of Salmonella typhi, or a group B Streptococcus polysaccharide.
22 . The method of any preceding claim wherein the polysaccharide can be selected from Meningococcal group A polysaccharide, a Meningococcal group C polysaccharide, a Meningococcal group W-135 polysaccharide, a Meningococcal group Y and a Meningococcal group X.
23. The method of any preceding claim wherein the polysaccharide can be selected from Pneumococcal group 1, 2, 3, 4, 5, 6A, 6B,7F,8, 9A, 9V, 9N, 10A, 11A , 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F,23F ,33F and 45.
24 . The method of any preceding claim wherein the carrier protein is a protein selected from the group consisting of tetanus toxoid (TT) , fragment C of TT, diphtheria toxoid, CRM197, Pneumolysin, protein D, PhtD, PhtDE,OMPC and N19.
25. A method of any preceeding claim,wherein the sample being assayed for 4-PPY comprises of atleast one conjugate selected from (a) a conjugate of (i) the capsular saccharide of serogroup A N meningitidis and (ii) tetanus toxoid; (b) a conjugate of (i) capsular saccharide of serogroup C N meningitidis and (ii) CRM197 ;
(c) a conjugate of (i) capsular saccharide of serogroup Y N. meningitidis and (ii) CRM197;(d) a conjugate of (i) capsular saccharide of serogroup W135 N.meningitidis and (ii)CRM197; and
(d) a conjugate of (i) capsular saccharide of serogroup X N meningitidis and (ii)tetanus toxoid.