The present invention relates generally to bathing compositions. More specifically, the present invention provides a rinse-free bathing composition that exhibits excellent cleansing activity, while being devoid of alcoholic solvents.
BACKGROUND
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art. [0003] Personal cleaning/bathing forms an essential part of daily routine for cleansing the body and removing the dirt and germs. Such compositions includes wet body baths (require rinsing with water) and rinse-free bathing compositions (require only wiping with a soft cloth). Among them, rinse-free compositions reduce or preclude use of water in cleansing the skin and other body parts and are of paramount importance to the people living in areas where water is scarce, including campers, backpackers, bedridden patients, defense personnel in remote areas, and the like.
[0004] However, existing rinse-free bathing compositions are largely inefficient in removing body odour, dirt, sweat, and germs. Further, unlike wet body baths, they leave behind residues that are difficult to remove. Moreover, the presence of harsh chemicals may cause skin rashes, itching and pH imbalance, leading to other skin problems. Further, the existing rinse-free bathing compositions contain strong fragrance and may make the skin dry, which may not be suitable for use on patients. So far, existence of such disadvantages has precluded large scale utilization/commercialization of the rinse-free bathing compositions.
[0005] While WO2019166865A1 (PCT/IB2018/052604), an application from the Applicant of the present disclosure, discloses a rinse free bathing composition, there are few shortcomings associated therewith, such as the composition disclosed therein being costly and not having desired disinfectant activity.

[0006] There is therefore, a need in the art for a rinse-free bathing composition that is devoid of, or at least alleviates, the disadvantages associated with the known rinse-free bathing compositions.
OBJECTS
[0007] An object of the present disclosure is to provide a rinse-free bathing
composition that satisfies the existing needs, as well as emerging ones, and
generally overcomes the deficiencies found in the prior art.
[0008] Another object of the present disclosure is to provide a rinse-free
bathing composition that is free from alcoholic solvents.
[0009] Another object of the present disclosure is to provide a rinse-free
bathing composition that is free from harsh chemicals.
[0010] Another object of the present disclosure is to provide a rinse-free
bathing composition that exhibits superior cleansing action over bodily surfaces.
[0011] Another object of the present disclosure is to provide a rinse-free
bathing composition that exhibits superior disinfectant activity.
[0012] Another object of the present disclosure is to provide a rinse-free
bathing composition that is economical and easy to manufacture.
[0013] Another object of the present disclosure is to provide a rinse-free
bathing composition that disinfect and leaves the skin moisturized post cleansing.
SUMMARY
[0014] The present invention relates generally to bathing compositions. More specifically, the present invention provides a rinse-free bathing composition that exhibits excellent cleansing activity, while being devoid of alcoholic solvents. [0015] The present disclosure is based, at least in part, on the premise of surprising observation of inventors of the present disclosure that a rinse-free bathing composition having a surfactant combination including cocamidopropyl betaine in an amount ranging from 0.8% to 1.2% w/w and capryl glucoside in an amount ranging from 0.8% to 1.2% w/w, exhibits dramatic improvement in foam maintenance time while retaining similar foaming index and cleansing efficacy in

comparison to a composition having a surfactant combination including cocamidopropyl betaine in an amount ranging from 1.3% to 1.9% w/w and capryl glucoside in an amount ranging from 1.3% to 1.8% w/w. Further, a composition having reduced amounts of surfactants (viz. cocamidopropyl betaine and capryl glucoside) confers economic advantage over compositions having higher amounts of surfactants. It was also surprising to note that inclusion of disinfectant modifier(s) such as citric acid, dehydroacetic acid and disodium EDTA dramatically improves the disinfectant activity of the composition. [0016] Accordingly, an aspect of the present disclosure provides a rinse-free bathing composition comprising: (a) a surfactant combination comprising cocamidopropyl betaine in an amount ranging from 0.8% to 1.2% by weight of the composition and capryl glucoside in an amount ranging from 0.8% to 1.2% by weight of the composition; (b) a disinfectant in an amount ranging from 0.05% to 1.5% by weight of the composition; (c) a disinfectant modifier in an amount ranging from 0.3% to 1.5% by weight of the composition, said disinfectant modifier being selected from citric acid, dehydroacetic acid, disodium EDTA and combinations thereof; and (d) an aqueous solvent in an amount ranging from 95% to 98%) by weight of the composition.
[0017] In an embodiment, the disinfectant is chlorhexidine or salt thereof. [0018] In an embodiment, the composition further includes a humectant in an amount ranging from 0.2% to 1.5% by weight of the composition. In an embodiment, the humectant is selected from a group including propylene glycol, glycerine, plant oils, lactic acid and panthenol.
[0019] In an embodiment, the composition further includes a preservative in an amount ranging from 0.01% to 1.5% by weight of the composition. In an embodiment, the preservative is selected from a group comprising sodium benzoate, dehydroacetic acid, citric acid, benzoic acid, sorbic acid, phenoxyethanol and analogues thereof.
[0020] In an embodiment, the composition is devoid of alcoholic solvent and paraben preservatives. In an embodiment, the composition further includes a

fragrance. In an embodiment, the fragrance is selected from the group consisting of limonene, linalool, citronellol and butylphenyl methylpropional. [0021] In an embodiment, the composition comprises: chlorhexidine digluconate in an amount ranging from 0.08%> to 0.4% by weight of the composition; citric acid in an amount ranging from 0.01%> to 0.05% by weight of the composition; and dehydroacetic acid in an amount ranging from 0.08%> to 0.5%) by weight of the composition.
[0022] In an embodiment, the composition comprises, by weight of the composition: propylene glycol - 0.5%; capryl glucoside - 1.01%; cocamidopropyl betaine - 1.1%; sodium benzoate - 0.2%; citric acid - 0.02%; dehydroacetic acid -0.2%; chlorhexidine digluconate - 0.12%; sodium hydroxide - 0.7%; fragrance -0.002%; and water in a quantity sufficient to make 100%).
[0023] Various objects, features, aspects and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiment/s, along with the accompanying drawing figures in which like numerals represent like components.
DETAILED DESCRIPTION
[0024] The following is a detailed description of embodiments of the disclosure depicted in the accompanying drawings. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.
[0025] Each of the appended claims defines a separate invention, which for infringement purposes is recognized as including equivalents to the various elements or limitations specified in the claims. Depending on the context, all references below to the "invention" may in some cases refer to certain specific embodiments only. In other cases it will be recognized that references to the

"invention" will refer to subject matter recited in one or more, but not necessarily all, of the claims.
[0026] As used in the description herein and throughout the claims that follow, the meaning of "a," "an," and "the" includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of "in" includes "in" and "on" unless the context clearly dictates otherwise.
[0027] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. "such as") provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention. [0028] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0029] The present invention relates generally to bathing compositions. More specifically, the present invention provides a rinse-free bathing composition. [0030] An aspect of the present disclosure provides a rinse-free bathing composition comprising: (a) a surfactant combination comprising cocamidopropyl betaine in an amount ranging from 0.8% to 1.2% by weight of the composition and capryl glucoside in an amount ranging from 0.8% to 1.2% by weight of the composition; (b) a disinfectant in an amount ranging from 0.05% to 1.5% by weight of the composition; (c) a disinfectant modifier in an amount ranging from 0.3%) to 1.5% by weight of the composition, said disinfectant modifier being selected from citric acid, dehydroacetic acid, disodium EDTA and combinations thereof; and (d) an aqueous solvent in an amount ranging from 95% to 98% by weight of the composition.

[0031] In an embodiment, the surfactant combination includes capryl
glucoside in an amount ranging from 0.5% to 1.3% by weight of the composition,
preferably, in an amount ranging from 0.8%> to 1.2% by weight of the
composition, more preferably, in an amount ranging from 0.9% to 1.2% by weight
of the composition, and most preferably, in an amount ranging from 0.9% to 1.1%
by weight of the composition.
[0032] In an embodiment, the surfactant combination includes
cocamidopropyl betaine in an amount ranging from 0.5% to 1.3% by weight of
the composition, preferably, in an amount ranging from 0.8% to 1.2% by weight
of the composition, more preferably, in an amount ranging from 0.9% to 1.2% by
weight of the composition, and most preferably, in an amount ranging from 1.0%
to 1.2% by weight of the composition.
[0033] In an embodiment, the disinfectant is present in an amount ranging
from 0.05%) to 1.5% by weight of the composition, preferably, in an amount
ranging from 0.07% to 1% by weight of the composition, more preferably, in an
amount ranging from 0.08% to 0.4% by weight of the composition, and most
preferably, in an amount ranging from 0.1% to 0.2% by weight of the
composition. In an embodiment, the disinfectant is present in an amount of about
0.12%w/w.
[0034] In an embodiment, the disinfectant is chlorhexidine or salt thereof such
as chlorhexidine acetate, chlorhexidine digluconate, chlorhexidine gluconate and
the likes. However, any other disinfectant, as known to a person skilled in the art,
can be utilized to realize the advantageous compositions of the present disclosure.
In an embodiment, the disinfectant is chlorhexidine digluconate.
[0035] In an embodiment, the composition further includes a humectant. The
humectants may be present in an amount ranging from 0.2% to 1.5% by weight of
the composition. Non-limiting examples of humectants that may be used for
realizing the composition of the present disclosure includes propylene glycol,
glycerine, plant oils, lactic acid and panthenol.
[0036] In an embodiment, the composition further includes a preservative.
The preservative may be present in an amount ranging from 0.01% to 1.5% by

weight of the composition. Non-limiting examples of preservatives that may be
used for realizing the composition of the present disclosure includes sodium
benzoate, dehydroacetic acid, citric acid, benzoic acid, sorbic acid,
phenoxyethanol and analogues thereof.
[0037] In an embodiment, the composition is devoid of alcoholic solvent and
paraben preservatives. In an embodiment, the composition further includes a
fragrance. Non-limiting examples of fragrance that may be used for realizing the
composition of the present disclosure includes limonene, linalool, citronellol and
butylphenyl methylpropional.
[0038] In an embodiment, the disinfectant modifier includes a combination of
citric acid and dehydroacetic acid.
[0039] In an embodiment, the composition includes: chlorhexidine
digluconate in an amount ranging from 0.08% to 0.4% by weight of the
composition; citric acid in an amount ranging from 0.01% to 0.05% by weight of
the composition; and dehydroacetic acid in an amount ranging from 0.08% to
0.5%) by weight of the composition.
[0040] In an embodiment, the composition includes, by weight of the
composition: propylene glycol - 0.5%; capryl glucoside - 1.01%; cocamidopropyl
betaine - 1.1%; sodium benzoate - 0.2%; citric acid - 0.02%; dehydroacetic acid -
0.2%; chlorhexidine digluconate - 0.12%; sodium hydroxide - 0.7%; fragrance -
0.002%; and water in a quantity sufficient to make 100%).
[0041] In an embodiment, the composition is devoid of or substantially devoid
of alcoholic solvents. In an embodiment, the composition is devoid of paraben
class of preservatives.
[0042] Preferably, the bathing compositions realized in accordance with
embodiments of the present disclosure are prepared in a semi-solid or a liquid
form with desired viscosity. Most preferably, the bathing compositions realized in
accordance with embodiments of the present disclosure are prepared in liquid
form. Nevertheless, the bathing compositions of the present disclosure can be
prepared in powdered form and the same are completely within the scope of the
present disclosure.

[0043] In an embodiment, the bathing compositions of the present disclosure exhibits a viscosity ranging from about 1 cps to about 2 cps, preferably between 1.5 cps to about 1.8 cps and most preferably, about 1.6 cps at room temperature (~30°C).
[0044] In an embodiment, the bathing compositions of the present disclosure exhibits pH ranging from about 6 to about 9, preferably, between 6.5 to about 8 and most preferably, about 6.9.
[0045] In an embodiment, the bathing compositions of the present disclosure has a total aerobic microbial count not more than 200 cfu/ml, preferably, the total aerobic microbial count is less than 100 cfu/ml and most preferably, total aerobic microbial count is less than 50 cfu/ml. In an embodiment, the bathing compositions of the present disclosure has total mould and yeast count less than 10 cfu/ml, preferably, less than 5 cfu/ml and most preferably, less than 3 cfu/ml. In a preferred embodiment, the bathing compositions of the present disclosure are free from harmful organisms such as E.coli, Salmonella and Pseudomonas. [0046] The rinse-free bathing compositions as realized in accordance with embodiments of the present disclosure can be used in any manner as known to a person skilled in the art. Preferably, the compositions can be directly applied to the bodily surfaces (skin) until the area is wet enough followed by gentle massaging. Subsequently, any cloth or sponge or wipe (dry or wet) can be utilized to clean the bodily surfaces to effect removal of dirt or soil and the bathing composition. The term "rinse-free bathing composition" as used herein throughout the present disclosure denotes the bathing or cleansing compositions that do not require utilization of water or require minimal amount of water (such as use of wet cloth or sponge or wipe) to effect removal of dirt or soil along with the composition.
[0047] The advantageous rinse-free bathing compositions as realized in accordance with embodiments of the present disclosure leave behind no residue, maintain the smoothness and the pH of the skin, do not cause skin dryness while completely cleansing the skin by removing dirt, sweat, germs and odor, provide excellent moisturizing effect and reduce the microbiological count.

[0048] While the foregoing describes various embodiments of the invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art. EXAMPLE
[0049] EXAMPLE 1 - Rinse-free bathing composition (Free of Alcohol and Parabens)

Propylene glycol 0.50%
Carpryl glucoside 1.01%
Cocamidopropyl betaine 1.10%
Sodium benzoate 0.20%
Citric acid 0.02%
Dehydroacetic acid 0.20%
Chlorhexidine digluconate 0.12%
Sodium hydroxide 0.71%
Fragrance (absolute repair) 0.00%
Water QS to make 100%
[0050] Foaming Index Measurement Apparatus
[0051] The Foaming indices for different compositions were determined with
the help of a cylinder based apparatus.
[0052] The apparatus includes 6 parallel cylinders, each with an internal
diameter of about 9 cm and a height of about 29 cm and are fixed to a rotating
frame occupying equivalent positions in the frame. Each cylinder is provided with
a graduated scale to measure the height of the foam of the composition under
investigation. These cylinders are closed at the top with the help of a removable
lid, wherein the lid is provided with a 5 mm hole to allow introduction of
additives (soil composition). The hole is closed with a rubber bunk, when the

cylinders are subjected to rotation. The frame holding the cylinders is rotated by an electric motor that rotates the fixed cylinders along an axis perpendicular to main axis of the cylinders, such that the cylinder is cut in the middle of their length, in the plane of the frame.
[0053] Composition under investigation move or flow in the cylinder during rotation, thereby generating a turbulence that leads to the formation of foam. Cylinders were rotated at a rotation speed of about 20 rpm, by series of 10 rotations (each lasting for about 30 seconds) followed by a rest of 3 minutes between each series to allow a measure of the foam height (at the end of the 3 minutes). The foam height is determined by reading the graduated scale when the cylinders are in a vertical position after rotation.
[0054] Protocol for measuring Foaming Index of the given composition: [0055] About 500 ml of composition to be investigated (maintained at 20 °C) was slowly poured into the cylinder by avoiding the formation of foam. The rotating frame holding the cylinders was rotated at a rotation speed of about 20 rotations per minute (rpm), by series of 10 rotations (each lasting for about 30 seconds) followed by a rest of 3 minutes between each series to allow a measure of the foam height (at the end of the 3 minutes). [0056] Compositions under investigation:
Table 1: Foam Height Measurement for compositions

Composition ID Composition Foam Height
1 1.01 % wt/wt of Capryl glucoside (in water) 5.0 cm
2 1.10% wt/wt of Cocamidopropyl Betaine (in water) 7.0 cm
3 1.01 % wt/wt of Capryl glucoside (in water) + 1.10% wt/wt of Cocamidopropyl Betaine (in water) 7.0 cm
4 1.8 % w/w of Capryl glucoside (in water) 8.0 cm
5 1.8 % w/w of Cocamidopropyl Betaine 8.3 cm

(in water)
6 1.8 % w/w of Capryl glucoside + 1.8% w/w of Cocamidopropyl Betaine (in water) 7.0 cm
[0057] Results presented as part of the Table 1 hereinabove clearly indicate
that a surfactant combination including capryl glucoside (0.8% to 1.2% by wt. of
the composition) and cocamidopropyl Betaine (0.8% to 1.2% by wt. of the
composition) exhibits a synergistic behaviour. Further, the tested surfactant
combination (composition ID 3) exhibited similar foaming index in comparison to
a combination including cocamidopropyl betaine in an amount of 1.8% w/w and
capryl glucoside in an amount of 1.8% w/w.
[0058] Foam Maintenance Index Measurement
[0059] The Foam Maintenance index of compositions were determined with
the help of a cylinder based apparatus utilized for foaming index determination
experiment.
[0060] About 500 ml of composition to be investigated (maintained at 20 °C)
was slowly poured into the cylinder by avoiding the formation of foam. The
rotating frame holding the cylinders was rotated at a rotation speed of about 20
rotations per minute (rpm), by series of 10 rotations (each lasting for about 30
seconds) followed by a rest of 3 minutes between each series to allow a measure
of the foam height (at the end of the 3 minutes).
[0061] To insure the availability of foam for a given period of time to serve its
purpose of cleansing; a foam maintenance test was conducted. For this, an
artificial soil composition was prepared for testing the bathing soap composition
effectiveness.
[0062] Composition of Artificial sebum: An artificial sebum composition
was used as an additive for measuring the sustainability of the foam formed in
foaming index apparatus described above. Artificial sebum (including a
combination of synthetic sebum and soil/clay) simulates greasy soils such as soils

coming from the human skin, mixed with clay (bentonite). The weight ratio
between synthetic sebum and clay is 10:4.
[0063] Composition for 850 g of synthetic sebum:
Table 2: Composition of Synthetic sebum

Sr. No. Ingredients Weight in gms
1 Palmitic acid 80
2 Stearic acid 40
3 Sebacid acid 45
4 Oleic acid 95
5 Linoleic acid 45
6 Paraffin wax (40-50°C) 90
7 Coconut oil (copprah) 95
8 Squalene 45
9 Mineral oil 180
10 Sojaoil 45
11 Colza oil 90
[0064] The above ingredients (synthetic sebum) were placed in a glass beaker and heated to a temperature of about 80°C for 15 minutes, under magnetic stirring to obtain a transparent and light colour liquid and can be stored for several weeks as solid material after cooling. For the foam maintenance evaluations, the desired artificial sebum composition was prepared by melting about 10 g of synthetic sebum at a temperature of about 80°C followed by addition of about 4g of clay, under magnetic stirring. [0065] Foam maintenance Test:
[0066] After the 10th series of (20 rotations + 3 minutes resting times), artificial sebum addition was effected by adding 5 droplets (about 0.15 g) of liquid and warm soil (80°C) into the cylinder. The cylinder was given 10 subsequent rotations at 20 rpm. The foam height was measured 3 minutes after the 10 rotations (total of 200+10=210 rotations). Repeat the artificial sebum addition implement with adding the artificial sebum just after the foam height

measure and just before the subsequent rotation. This rotation, wait, measure, artificial sebum addition implement was repeated, until the foam height reaches a value of about 1.6 cm. Foam Maintenance Time (in seconds) required for foam to subside (i.e. decrease in height of foam) from initial 1.6 cm to 1.0 cm was measured in seconds. More time required to subside the foam indicates the stability of the composition to effectively clean the soil/dirt that need to cleaned from the human skin.
Table 3: Foam Maintenance Time (in seconds) for compositions

Composition ID Composition Foam Maintenance Time (Seconds)
1. 1.01 % wt/wt of Capryl glucoside (in
water) +1.10% wtAwt of Cocamidopropyl Betaine (in water) 108
2. 1.8 % w/w of Capryl glucoside + 1.8%
w/w of Cocamidopropyl Betaine (in
water) 98
3. Composition as prepared in Example 1 107
[0067] Results presented as part of the Table 1 and Table 3 hereinabove clearly indicate that a surfactant combination including capryl glucoside (0.8% to 1.2%) by wt. of the composition) and cocamidopropyl betaine (0.8%> to 1.3% by wt. of the composition) exhibits a synergistic behavior. Particularly, a surfactant combination including cocamidopropyl betaine in an amount of 1.10% w/w and capryl glucoside in an amount of 1.01% w/w, exhibits dramatic improvement in foam maintenance time in comparison to a composition having a surfactant combination including cocamidopropyl betaine in an amount of 1.8% w/w and capryl glucoside in an amount of 1.8% w/w.
[0068] Anti-microbial/disinfectant activity of the composition [0069] The composition of Example 1 was tested for its anti-microbial activity by a time kill assay. The assay notes the changes in the microbial population within a specified time in presence of the composition. The time kill assays were

performed on the ready-to-use formulation. The assays were performed as per the American Standard for Testing and Material protocol ASTM-E2315-16. [0070] Test Procedure: The working cultures of the test organisms were prepared. 10 mL of the sample was taken with respect to individual test culture. 100 uL of adjusted test cultures were inoculated in individual samples prepared. A contact time of 20-60 seconds was considered. Using the pour plate technique 1 mL of the formulation was pipetted to triplicated petri dishes for each dilution prepared by serial dilution technique and then 20mL of melted SCDA was added. The petri dish was incubated at 30-35 °C for 72 hours. The colonies on the petri dishes were counted with the help of colony counter after incubation and the observations were recorded in cfu (colony forming unit). The petri dish showing a number of colonies included in a 15-300 for bacterial and fungal of 15-150 range was used to perform the result calculation.
[0071] Log Reduction Calculation: The effectiveness of the disinfection procedure against each challenge organism was calculated for each product as a logio reduction as follows:
Logio Reduction per product = Logio [Positive Control Count] - Logio [Test
Surface Count]
(The log reduction was rounded to the nearest 0.1 value). For each product
concentration and each experimental condition, calculated and recorded the
decimal log reduction separately.
[0072] Acceptance Criteria: In case of surface challenge test positive control
should yield 50 to 200% recovery of the microorganism in comparison to
inoculums added. Bactericidal, fungicidal or yeasticidal activity on surfaces for
general purposes is characterized by the concentration of the tested product.
Agents should demonstrate a 3 log reduction of vegetative test organisms and
mold spores.
[0073] The results of the test are provided in Table 4 below:

Table 4: Anti-microbial activity of the composition of Example 1

S.No. Organism Contact time % reduction
1 Candida L albicans(MTCC 227) 30 Sec 99.93%

IMin 100.00%

5Min 100.00%
2 S aureus (ATCC 6538) 30 Sec 99.92%

IMin 100.00%

5Min 100.00%
3 Staph. Epidermis (ATCC12228) 30 Sec 99.98%

IMin 100.00%

5Min 100.00%
4 Escherichia coli (ATCC 8739) 30 Sec 99.99%

IMin 100.00%

5Min 100.00%
6 Aspergillus brasilensis (ATCC 16404) 30 Sec 99.95%

IMin 100.00%

5Min 100.00%
7 P.acnes (MTCC 1951) 30 Sec 99.98%

IMin 100.00%

5Min 100.00%
[0074] The above results clearly indicate that the composition of the present invention provides quick action as the anti-microbial efficacy was about 99.9% within 30 seconds of use. Additionally, the composition shows broad spectrum activity with triple action viz. antibacterial, antifungal and sporicidal activity. [0075] Evaluation of synergy of antimicrobial activity [0076] Disk-diffusion test: The disinfectant activity was evaluated by Disk diffusion test. In this study, a disk diffusion test was performed on Staph.

Epidermis (ATCC12228) using the composition of Example 1 and some comparative compositions as shown in Table 5 below.
Table 5: Disk-diffusion tests

0 Component Antimicrobial Inhibition Zone (cm)
Chlorohexidine Digluconate (0.12%) 3.0
Chlorohexidine Digluconate (0.12%) + Dehydroacetic Acid (0.2 %) 3.0
. Chlorohexidine Digluconate (0.12%) + Citric Acid (0.02 %) 3.0
\. Chlorohexidine Digluconate (0.12%) + Dehydroacetic Acid (0.2%) + Citric Acid (0.02%) 4.8
. Composition of Example 1 4.8
). Composition of WO2019166865A1 (provided therein in Example 1) 3.4
[0077] It could be concluded from the results that disinfectant activity of chlorohexidine digluconate was significantly increased by addition of dehydroacetic acid and citric acid in the specific weight percentage ranges and the composition shows synergistic effect. Surprisingly, there was no increase in antimicrobial inhibition zone upon addition of dehydroacetic acid only and similarly for addition of only citric acid. Whereas in combination of chlorohexidine digluconate, citric acid, and dehydroacetic acid, the antimicrobial/disinfectant activity increased significantly. Therefore, it can be concluded that the activity of chlorohexidine digluconate accelerated in the presence of disinfectant modifiers citric acid and dehydroacetic acid.
ADVANTAGES
[0078] The present disclosure provides a rinse-free bathing composition that
overcomes the deficiencies associated with the existing rinse-free bathing
composition.
[0079] The present disclosure provides a rinse-free bathing composition that
is free from alcoholic solvents.

[0080] The present disclosure provides a rinse-free bathing composition that
is free from harsh chemicals.
[0081] The present disclosure provides a rinse-free bathing composition that
exhibits superior cleansing action over bodily surfaces.
[0082] The present disclosure provides a rinse-free bathing composition that
exhibits superior disinfectant activity.
[0083] The present disclosure provides a rinse-free bathing composition that
is economical and easy to manufacture.
[0084] The present disclosure provides a rinse-free bathing composition that
disinfect and leaves the skin moisturized post cleansing.

We Claim:

1. A rinse-free bathing composition comprising:
a surfactant combination comprising cocamidopropyl betaine in an
amount ranging from 0.8% to 1.2% by weight of the composition and
capryl glucoside in an amount ranging from 0.8% to 1.2% by weight of
the composition;
a disinfectant in an amount ranging from 0.05% to 1.5% by weight of
the composition;
a disinfectant modifier in an amount ranging from 0.3% to 1.5% by
weight of the composition, said disinfectant modifier being selected
from citric acid, dehydroacetic acid, disodium EDTA and
combinations thereof; and
an aqueous solvent in an amount ranging from 95% to 98% by weight
of the composition.
2. The composition as claimed in claim 1, wherein the disinfectant is chlorhexidine or salt thereof.
3. The composition as claimed in claim 1, wherein the composition further comprises a humectant in an amount ranging from 0.2% to 1.5% by weight of the composition.
4. The composition as claimed in claim 3, wherein the humectant is selected from a group comprising propylene glycol, glycerine, plant oils, lactic acid and panthenol.
5. The composition as claimed in claim 1, wherein the composition further comprises a preservative in an amount ranging from 0.01% to 1.5% by weight of the composition.
6. The composition as claimed in claim 5, wherein the preservative is selected from a group comprising sodium benzoate, dehydroacetic acid, citric acid, benzoic acid, sorbic acid, phenoxyethanol and analogues thereof;

7. The composition as claimed in claim 1, wherein the composition is devoid of alcoholic solvent and paraben preservatives.
8. The composition as claimed in claim 1, wherein the composition further comprises a fragrance.
9. The composition as claimed in claim 1, wherein the composition comprises: chlorhexidine digluconate in an amount ranging from 0.08% to 0.4% by weight of the composition; citric acid in an amount ranging from 0.01% to 0.05% by weight of the composition; and dehydroacetic acid in an amount ranging from 0.08% to 0.5% by weight of the composition.
10. The composition as claimed in claim 1, wherein the composition comprises, by weight of the composition:
propylene glycol - 0.5%;
capryl glucoside - 1.01%;
cocamidopropyl betaine - 1.1%;
sodium benzoate - 0.2%;
citric acid - 0.02%;
dehydroacetic acid - 0.2%;
chlorhexidine digluconate - 0.12%;
sodium hydroxide - 0.7%;
fragrance - 0.002%; and
water in a quantity sufficient to make 100%.