[0001] The present disclosure relates to a sanitizing composition. Specifically, the present disclosure relates to a sanitizing composition comprising anti-microbial agents denatured alcohol and isopropyl alcohol. The present disclosure further relates to a process for preparing the sanitizing composition.

BACKGROUND OF THE INVENTION
[0002] The background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] Human beings are constantly exposed to a host of microbes in the environment. Some of them are useful for example, Azotobacter which helps fix atmospheric nitrogen, or Lactobacillus which is a pro-biotic bacterium that is a friendly occupant of the gut. On the other hand some are harmful for example, Staphylococci which is responsible for multiple infections, Rhinovirus which is the culprit of common cold, or P. falciparum known for causing malaria etc. These micro-organisms are undetectable by the eye and therefore humans are unaware of their levels of exposure. Hence, sanitizing the exposed or compromised regions for example our hands, feet etc. should be an essential and unavoidable part of our hygiene. For this water alone may not be enough and there is a requirement for use of additional sanitizing solutions.
[0004] Many sanitizing compositions are available in the market, but most of these make use of toxic chemicals that perform as surfactants, anti-microbials and preservatives, namely sodium lauryl sulfate (SLS), triclosans, octenidine hydrochloride, PVP, hydrogen peroxide, parabens, hypochlorite, and other synthetic harmful chemicals.
[0005] Alcohol is very widely known as an antiseptic and in recent times commercialization of sanitizers with alcohol has seen a significant growth. Alcohol-based sanitizers are effective against a number of microorganisms. However, there is a dearth in the industry of sanitizing compositions that are gentle on the skin and are still effective broad-spectrum anti-microbials.
[0006] The inventors of the present disclosure provide a sanitizing composition that provides protection against microbes while being gentle to the skin and overcome the drawbacks in the art.

OBJECTS OF THE INVENTION
[0007] An object of the present disclosure is to provide a sanitizing composition.
[0008] An object of the present disclosure is to provide a sanitizing composition that has a broad spectrum anti-microbial activity.
[0009] An object of the present disclosure is to provide a sanitizing composition which provides protection from virus, bacteria, and fungi.
[0010] An object of the present disclosure is to provide a sanitizing composition that is skin friendly.
[0011] An object of the present disclosure is to provide a sanitizing composition free of harsh chemicals like SLS, hydrogen peroxide, hypochlorite, parabens, and titanium dioxide.
[0012] Another object of the present disclosure is to provide a process of preparing a sanitizing composition.

SUMMARY OF THE INVENTION
[0013] This summary is provided to introduce a selection of concepts in a simplified form that are further described below in Detailed Description section. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
[0014] Aspects of the present disclosure relate to a sanitizer composition comprising anti-microbial agents that provide a synergistic anti-microbial effect against a broad spectrum of microbes and are skin-friendly.
[0015] In an aspect, the present disclosure relates to a sanitizing composition comprising anti-microbial agents, wherein the anti-microbial agents are denatured alcohol and 2-propanol or isopropyl alcohol. The anti-microbials may be present in specific weight percentage range with respect to the composition.
[0016] In an embodiment, the sanitizing composition comprises anti-microbial agents: a) denatured alcohol in a weight percentage range of about 60% to about 80% of the composition; and b) isopropyl alcohol in a weight percentage range of about 1% to about 5% of the composition.
[0017] In an embodiment, the composition further comprises an emollient or skin hydrating agent.
[0018] In an embodiment, the emollient or skin hydrating agent may be selected from propylene glycol, butylene glycol, cocoa butter, glycerine, niacinamide, Aloe vera, hydroxypropyl methylcellulose or combinations thereof. In an embodiment, the emollient or skin hydrating agent may be present in a weight percentage range of about 0.1% to about 6% of the composition.
[0019] In an embodiment, the composition further comprises an emulsifier.
[0020] In an embodiment, the emulsifier may be selected from a polyacrylic acid, hydrogenated castor oil, xanthan gum, guar gum or combinations thereof. The polyacrylic acid may be carbomer. In an embodiment, the emulsifier may be present in a weight percentage range of about 0.1% to about 10% of the composition.
[0021] In an embodiment, the composition further comprises a preservative.
[0022] In an embodiment, the preservative may be selected from butylated hydroxytoluene, citric acid, benzoic acid, sodium benzoate or combinations thereof. In an embodiment, the preservative may be present in a weight percentage range of about 0.01% to about 1.0% of the composition.
[0023] In an embodiment, the composition further comprises aloe gel. The aloe gel may be extracted from Aloe barbadensis (miller), Aloe vera, Aloe petricola, Aloe ciliaris, Aloe ferox, Aloe striata, Aloe aristata, Aloe arborescens, Aloe humilis, or combinations thereof.
[0024] In an embodiment, the aloe gel may be present in a weight percentage range of about 0.01% to about 3% of the composition.
[0025] In an embodiment, the composition further comprises buffering agent. The buffering agent may be selected from triethanolamine, sodium hydroxide, sodium bicarbonate, or combinations thereof.
[0026] In an embodiment, the buffering agent may be present in a weight percentage range of about 0.01% to about 3% of the composition.
[0027] In an aspect, the present disclosure provides a sanitizing formulation comprising denatured alcohol in a weight percentage range of about 60% to about 80% of the formulation; isopropyl alcohol in a weight percentage range of about 1% to about 5% of the formulation, an emollient or skin hydrating agent, an emulsifier, aloe gel, a preservative, a buffering agent, and an excipient.
[0028] The excipient may be selected from, but is not limited to, thickeners, perfumes, coloring agent, solvent or combinations thereof.
[0029] In an embodiment, the thickener may be selected from polyethylene glycol, hydroxypropyl methylcelluose, guar gum, xanthan gum, carbomer or combinations thereof.
[0030] In an embodiment, the perfume is selected from lemon, rose, sandalwood, lavender, jasmine, chamomile, lemongrass, menthol, mint, rosemary, mogra or combinations thereof.
[0031] In an embodiment, the coloring agent maybe selected from indigo blue color, sandalwood color, green chlorophyll color, light blueish color, sky blue color, or combinations thereof.
[0032] In an aspect, the present disclosure relates to a process of preparing a sanitizing composition, wherein the process comprises the steps of: (a) adding denatured alcohol and isopropyl alcohol in distilled water with constant stirring; (b) mixing butylated hydroxytoluene, propylene glycol, an aloe gel and hydrogenated castor oil; (c) blending the mixtures of step (a) and step (b) at 400 rpm to 1000 rpm; (d) adding polyacrylic acid to step (c) with constant stirring at 400rpm to 1000 rpm and heating at 30°C; (e) adding a buffering agent at 400 rpm to 1200 rpm at room temperature; and (f) optionally introducing a coloring agent and perfume and making up the volume with distilled water.
[0033] Other aspects of the invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learnt by the practice of the invention.

BRIEF DESCRIPTION OF THE DRAWINGS
[0034] The following drawings form part of the present specification and are included to further illustrate aspects of the present disclosure. The disclosure may be better understood by reference to the drawings in combination with the detailed description of the specific embodiments presented herein.
Figure 1 provides an image of the microbial growth in a dirty hand swab sample. Figure 1(a) shows the growth of microorganisms in dirty hand swab sample before treatment, and Figure 1(b) shows no microorganism in the dirty hand swab sample after application of the formulation as per an embodiment of the present disclosure.
Figure 2 provides the results of disc diffusion test in a petri dish for the formulation as per an embodiment of the present disclosure. Figure 2(a) shows the anti-microbial inhibition zone (clear zone in the centre of the petri dish) created by the formulation and Figure 2(b) shows no inhibition zone in the sample without the formulation.

DETAILED DESCRIPTION OF THE INVENTION
[0035] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.
[0036] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0037] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0038] In some embodiments, numbers have been used for quantifying amounts, weights, temperatures, and so forth, to describe and claim certain embodiments of the invention and are to be understood as being modified in some instances by the term “about.” Accordingly, in some embodiments, the numerical parameters set forth in the written description and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable. The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.
[0039] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0040] As used in the description herein and throughout the claims that follow, the meaning of “a,” “an,” and “the” includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of “in” includes “in” and “on” unless the context clearly dictates otherwise.
[0041] Unless the context requires otherwise, throughout the specification which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including, but not limited to.”
[0042] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.
[0043] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.
[0044] Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limitations. Each group member can be referred to and claimed individually or in any combination with other members of the group or other elements found herein. One or more members of a group can be included in, or deleted from, a group for reasons of convenience and/or patentability. When any such inclusion or deletion occurs, the specification is herein deemed to contain the group as modified.
[0045] The description that follows, and the embodiments described therein, is provided by way of illustration of an example, or examples, of particular embodiments of the principles and aspects of the present disclosure. These examples are provided for the purposes of explanation, and not of limitation, of those principles and of the disclosure.
[0046] It should also be appreciated that the present disclosure can be implemented in numerous ways, including as a system, a method or a device. In this specification, these implementations, or any other form that the invention may take, may be referred to as processes. In general, the order of the steps of the disclosed processes may be altered within the scope of the invention.
[0047] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[0048] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.
[0049] The present disclosure relates to a sanitizer composition. Specifically, the present disclosure relates to a sanitizer composition having anti-microbial effect and is gentle on the skin.
[0050] In an embodiment, the present disclosure provides a sanitizing composition comprising anti-microbial agents, wherein the anti-microbial agents are denatured alcohol and 2-propanol or isopropyl alcohol. The anti-microbials may be present in specific weight percentage range with respect to the composition.
[0051] In an embodiment, the sanitizing composition comprises anti-microbial agents: a) denatured alcohol in a weight percentage range of about 60% to about 80% of the composition; and b) isopropyl alcohol in a weight percentage range of about 1% to about 5% of the composition.
[0052] In a preferred embodiment, the sanitizing composition of the present disclosure comprises denatured alcohol in a weight percentage of about 70% and isopropyl alcohol in a weight percentage of about 3%. Surprisingly, the inventors have found that the composition comprising denatured alcohol and isopropyl alcohol in the above ratio, which has not been reported earlier, leads to an increase in efficacy and stability of the sanitizing composition due to mutual regulation. The antimicrobial activity of the denatured alcohol is increased significantly in the presence of isopropyl alcohol in the specific weight percentages.
[0053] In an embodiment, the composition further comprises an emollient or skin hydrating agent.
[0054] In an embodiment, the emollient or skin hydrating agent may be selected from propylene glycol, butylene glycol, cocoa butter, glycerine, niacinamide, Aloe vera, hydroxypropyl methylcellulose, or combinations thereof. In an embodiment, the emollient or skin hydrating agent may be present in a weight percentage range of about 0.1% to about 6% of the composition. However, a person skilled in the art would appreciate that any other emollient(s) or skin hydrating agent(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention
[0055] In an embodiment, the composition further comprises an emulsifier.
[0056] In an embodiment, the emulsifier may be selected from a polyacrylic acid, hydrogenated castor oil, xanthan gum, guar gum, or combinations thereof. The polyacrylic acid may be carbomer. In an embodiment, the emulsifier may be present in a weight percentage range of about 0.1% to about 10% of the composition. However, a person skilled in the art would appreciate that any other emulsifier(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0057] In an embodiment, the composition further comprises a preservative.
[0058] In an embodiment, the preservative may be selected from butylated hydroxytoluene, citric acid, benzoic acid, sodium benzoate or combinations thereof. In an embodiment, the preservative may be present in a weight percentage range of about 0.01% to about 1.0% of the composition. However, a person skilled in the art would appreciate that any other preservative(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0059] In an embodiment, the composition further comprises aloe gel. The aloe gel may be extracted from Aloe barbadensis (miller), Aloe vera, Aloe petricola, Aloe ciliaris, Aloe ferox, Aloe striata, Aloe aristata, Aloe arborescens, Aloe humilis, or combinations thereof. However, a person skilled in the art would appreciate that any other aloe plant can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0060] In an embodiment, the aloe gel may be present in a weight percentage range of about 0.01% to about 3% of the composition.
[0061] In an embodiment, the composition further comprises a buffering agent. The buffering agent may be selected from triethanolamine, sodium hydroxide, sodium bicarbonate or combinations thereof. However, a person skilled in the art would appreciate that any other buffering agent(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0062] In an embodiment, the buffering agent may be present in a weight percentage range of about 0.01% to about 3% of the composition.
[0063] In an aspect, the present disclosure provides a sanitizing formulation comprising denatured alcohol in a weight percentage range of about 60% to about 80% of the formulation; isopropyl alcohol in a weight percentage range of about 1% to about 5% of the formulation, an emollient or skin hydrating agent, an emulsifier, aloe gel, a preservative, a buffering agent, and an excipient.
[0064] The excipient may be selected from, but is not limited to, thickeners, perfumes, coloring agent, solvent or combinations thereof. The excipient and its amount used may be altered as per requirement in a formulation.
[0065] In an embodiment, the thickener may be selected from polyethylene glycol, hydroxypropyl methylcelluose, guar gum, xanthan gum, carbomer, or combinations thereof. However, a person skilled in the art would appreciate that any other thickener(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0066] In an embodiment, the perfume is selected from lemon, rose, sandalwood, lavender, jasmine, chamomile, lemongrass, rosemary, mogra or combinations thereof. In an embodiment, the perfume is any fragrance suitable for a sanitizer. In a preferred embodiment, the perfume may be present in about 0.35% by weight of the formulation. However, a person skilled in the art would appreciate that any other perfume(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0067] In an embodiment, the coloring agent maybe selected from indigo blue color, sandalwood color, green chlorophyll color, light blueish color, sky blue color, or combinations thereof. In a preferred embodiment, the coloring agent may be present in about 0.01% by weight of the formulation. However, a person skilled in the art would appreciate that any other coloring agent(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0068] In an embodiment, the solvent may be selected from water, de-mineralised water, distilled water, or combinations thereof. The solvent that is used to make up the volume of the formulation. In a preferred embodiment, the sanitizing formulation may comprise distilled water in about 30% to about 60% by weight of the formulation.
[0069] In an embodiment, the sanitizing formulation may be present in the form of a gel or liquid or aerosol.
[0070] In some embodiments, the formulation may be a liquid. In an embodiment, the sanitizing formulation may have a viscosity in the range of about 1 CPS to about 10000 CPS.
[0071] In an embodiment, the sanitizing composition has a long shelf-life. The sanitizing composition is kept sealed or air tight except for during usage.
[0072] In an embodiment, the composition is a hand sanitizing composition. The hand sanitizing composition maybe released via a dispenser and may evaporate within minutes of coming in contact with a surface.
[0073] The sanitizing composition may be used for various industrial or domestic applications. In an embodiment, the dispenser maybe placed in a house, hospital, workplace, airports, buses, trains, health centers, schools, or any place where there is a risk of exposure to microbes.
[0074] In an embodiment, the sanitizing composition maybe used on skin or may be applied on appliances including laptops, door knobs, phones, screens, table tops, desks, rails, gym equipments, toilets, faucets, or handles.
[0075] In an embodiment, the sanitizing composition maybe used on any part of the skin exposed to external microbes.
[0076] In some embodiments, the sanitizing composition may be applied on a surface and then rubbed well for a few seconds to ensure its anti-microbial effect on the microbes on the surface. The composition does not require water for use and therefore can be used anywhere without water limitations. The sanitizing composition reduces the risk of infection by reducing the micro-organisms of the surface.
[0077] In an embodiment, the sanitizing composition may be used directly, alternatively it may be used after dilution or using a wipe or cloth. Preferably, the sanitizing composition is used directly.
[0078] In an embodiment, the sanitizing composition is a broad spectrum anti-microbial. The sanitizing composition is effective against protection from virus, bacteria, bacterial spores, and fungi.
[0079] In an embodiment, the sanitizing composition is effective against Esherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Enterococcus faecalis, Streptococcus pneumonia, and Candida albicans.
[0080] The composition is skin-friendly, not just sanitizes but also moisturizes the skin. The sanitizing composition is free of harsh chemicals including, SLS, hydrogen peroxide, hypochlorite, parabens and titanium dioxide.
[0081] The sanitizing composition has quick or fast action. In some embodiments, the sanitizing composition is capable of killing majority of the microbes in 15-30 seconds.
[0082] In an embodiment, the present disclosure further relates to a process of preparing a sanitizing composition, wherein the process comprises the steps of: (a) adding denatured alcohol and isopropyl alcohol in distilled water with constant stirring; (b) mixing butylated hydroxytoluene, propylene glycol, an aloe gel and hydrogenated castor oil; (c) blending the mixtures of step (a) and step (b) at 400 rpm to 1000 rpm; (d) adding polyacrylic acid to step (c) with constant stirring at 400rpm to 1000 rpm and heating at 30°C; (e) adding the buffering agent at 400 rpm to 1200 rpm at room temperature; and (f) optionally introducing a coloring agent and perfume and making up the volume with distilled water.
[0083] Although the subject matter has been described in considerable detail with reference to certain preferred embodiments thereof, other embodiments are possible.
EXAMPLES
[0084] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
MATERIALS: All the chemicals for the purposes of the present example were obtained from P.K. Scientific companies and were industrial grade.
EXAMPLE 1: Sanitizing Formulation
[0085] The sanitizing formulation was prepared as per the composition provided in Table No. 1 below. In the first step, denatured alcohol, and isopropyl alcohol were added into 10 mL of distilled water with constant stirring at 200 rpm and 25 °C to give the first mixture. Butylated hydroxytoluene, propylene glycol, aloe vera gel, and Cresmer® RH 40 were mixed to give the second mixture. Both the mixtures were then blended with constant mixing at 1000 rpm. In the fourth step, carbomer was added with constant stirring at 1000 rpm and was heated at 30 °C. Triethanolamine was added to the above mixture with constant stirring at 800-1200 rpm at room temperature. Brilliant blue and perfume were mixed separately in water and thereafter mixed with the formulation. The total volume was made up to 100 % using distilled water.

Table No. 1: Components for sanitizing formulation
Component Weight percentage (%)
Denatured alcohol 70.00
2-propanol 3.00
Propylene glycol 1.00
Carbomer 0.50
Triethanolamine 0.20
Butylated hydroxyltoluene 0.02
Aloe vera gel 0.30
Cresmer RH® 40 0.40
Natural Boutique (perfume) 0.35
Cyan color 8 0.01
Distilled water Q.S

EXAMPLE 2: Anti-microbial efficacy test of the formulation
[0086] 2.1 Disc diffusion on dirty hand swab: Anti-microbial activity of the formulation of Example 1 was tested on dirty hand swab samples. The anti-bacterial activity was assessed by disc diffusion test by following Standard Protocol No.: CIPL/Micro/001/R001, in vitro test for microbial inhibition. Figure 1(a) shows the growth of microorganisms in dirty hand swab samples before treatment with the formulation, and Figure 1(b) shows no microorganism growth in the dirty hand swab sample after application of the formulation of Example 1. Figure 2(a) shows the anti-microbial inhibition zone (clear zone in the centre of the petri dish) created by the formulation of Example 1 in the hand swab sample after the disc diffusion test and Figure 2(b) shows no inhibition zone in the hand swab sample without the formulation. The zone of inhibition created by the present formulation in the dirty hand swab sample was 3.4cm.
[0087] 2.2 Well-diffusion method on multiple organisms: Antimicrobial activity of the composition of Example 1 was tested against 9 different microorganisms recommended by FDA. In the well diffusion method, 100 µL of the formulation of Example 1 was incubated against the specified microorganisms for 12-14 hours at 37-degree Celsius and thereafter zone of antimicrobial inhibition was analyzed. The results are presented in Table No. 2 below.
Table No. 2: Results for well-diffusion method
Microorganism Zone of inhibition (cm)
Esherichia coli 2.8
Klebsiella pneumonia 2.8
Pseudomonas aeruginosa 2.8
Staphylococcus aureus 2.7
Staphylococcus epidermidis 2.7
Streptococcus pyogenes 2.7
Enterococcus faecalis 2.9
Streptococcus pneumonia 2.9
Candida albicans 3.0

[0088] The results confirm the broad-spectrum anti-microbial effect of the formulation of the present disclosure.
[0089] 2.3 Comparative activity against Pseudomonas aeruginosa
[0090] The anti-microbial activities of different compositions comprising the active components as disclosed in Table No. 3 were tested against Pseudomonas aeruginosa using disc diffusion test. Zone of inhibitions of the compositions was noted to compare the anti-microbial effect of the present composition. 100 µl of composition was incubated against Pseudomonas aeruginosa for 12-14 hours at 37-degree Celsius and thereafter zone of antimicrobial inhibition was analyzed.
Table No. 3: Comparative anti-microbial activity against Pseudomonas aeruginosa
Composition Zone of inhibition (cm)
Isopropanol 3.0% 2.0
Denatured alcohol 70.0% 2.4
Isopropanol 3.0% + Denatured alcohol 70.0%
(Antimicrobial activity with only active ingredients of present composition) 2.8
Isopropanol 3.0% + Denatured alcohol 70.0%
(formulation as per Example 1) 3.0

[0091] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.

ADVANTAGES OF THE PRESENT INVENTION
[0092] The present disclosure provides a sanitizing composition with a broad spectrum of anti-microbial effect.
[0093] The present disclosure provides a sanitizing composition that is skin-friendly and is free of harsh chemicals SLS, hydrogen peroxide, hypochlorite, titanium dioxide and parabens.

WE CLAIM

A sanitizing composition comprising a) denatured alcohol in a weight percentage range of 60% to 80% of the composition; and b) isopropyl alcohol in a weight percentage range of 1% to 5% of the composition.
2. The composition as claimed in claim 1, wherein the composition further comprises an emollient or skin hydrating agent selected from propylene glycol, butylene glycol, cocoa butter, glycerine, niacinamide, Aloe vera, hydroxypropyl methylcellulose or combinations thereof.
3. The composition as claimed in claim 2, wherein the emollient or skin hydrating agent is present in a weight percentage range of 0.1% to 6% of the composition.
4. The composition as claimed in claim 1, wherein the composition further comprises an emulsifier selected from a polyacrylic acid, hydrogenated castor oil, xanthan gum, guar gum or combinations thereof.
5. The composition as claimed in claim 4, wherein the emulsifier is present in a weight percentage range of 0.1% to 10% of the composition.
6. The composition as claimed in claim 1, wherein the composition further comprises a preservative selected from butylated hydroxytoluene, citric acid, benzoic acid, sodium benzoate or combinations thereof.
7. The composition as claimed in claim 6, wherein the preservative is present in a weight percentage range of 0.01% to 1.0% of the composition.
8. The composition as claimed in claim 1, wherein the composition further comprises aloe gel extracted from Aloe barbadensis (miller), Aloe vera, Aloe petricola, Aloe ciliaris, Aloe ferox, Aloe striata, Aloe aristata, Aloe arborescens, Aloe humilis, or combinations thereof.
9. The composition as claimed in claim 8, wherein the aloe gel is present in a weight percentage range of 0.01% to 3% of the composition.
10. The composition as claimed in claim 1, wherein the composition further comprises a buffering agent selected from triethanolamine, sodium hydroxide, sodium bicarbonate or combinations thereof.
11. The composition as claimed in claim 10, wherein the buffering agent is present in a weight percentage range of 0.01% to 3% of the composition.
12. The composition as claimed in claim 1, wherein the composition is a hand sanitizing composition.
13. A sanitizing formulation comprising denatured alcohol in a weight percentage range of 60% to 80% of the formulation; isopropyl alcohol in a weight percentage range of 1% to 5% of the formulation, an emollient or skin hydrating agent, an emulsifier, aloe gel, a preservative, a buffering agent, and an excipient.
14. A process of preparing a sanitizing composition, wherein the process comprises the steps of: (a) adding denatured alcohol and isopropyl alcohol in distilled water with constant stirring; (b) mixing butylated hydroxytoluene, propylene glycol, an aloe gel and hydrogenated castor oil; (c) blending the mixtures of step (a) and step (b) at 400 rpm to 1000 rpm; (d) adding polyacrylic acid to step (c) with constant stirring at 400rpm to 1000 rpm and heating at 30°C; (e) adding the buffering agent at 400 rpm to 1200 rpm at room temperature; and (f) optionally introducing a coloring agent and perfume and making up the volume with distilled water.