FIELD OF INVENTION
The present embodiment relates to a method and a device for spreading a liquid on a glass slide, and more particularly relates to the semi-automated device and method for preparation of the blood smears on the glass slides.
BACKGROUND OF THE INVENTION
A blood smear is a diagnostic test that is employed in the diagnosis of an abnormality in various components of blood. The blood smear is used in the diagnosis of a variety of diseases such as, but not limited to, anaemia, thalassemia, infections and leukaemia.
The haematological analysis involves the following steps: 1) Preparation of a glass slide by spreading a drop of blood. 2) Staining of the smear through a variety of stains/dyes. 3) Analysis of the desired parameter/blood component through a microscope.
Traditionally, the blood smear is prepared by placing a drop of blood on a glass slide and then spreading the blood by a spreader slide. The spreader slide is placed at an angle of 30°-45° from the glass slide and is moved front-to-back with a uniform speed to spread the drop of blood. The method has a various disadvantages vis-a-vis increased risk of contamination and inconsistency in the length and thickness of the smears.
Currently, a variety of automated devices are available for spreading the blood drop on the glass slide. The automated devices are either based on a principle of rotation of the glass slide or continuous variation of the velocity and angle of the slide. These methods have many disadvantages such as, but not limited to, increased risk of scattering of blood, additional power source and non-uniform thickness and length

of the smears. Further, the automated devices are complex and expensive and require trained technicians for the preparation of the glass slides. Furthermore, the available automated devices are configured to prepare a single slide at a time and requires a continuous source of power supply.
Therefore, there is a need of a semi-automated device and method that overcomes the shortcomings of the prior art and that does not depend on a power supply for the functioning. Further, there is a need of the semi-automated device and method that is able to prepare multiple slides having a uniform length and thickness of the
OBJECTIVE OF THE INVENTION
The main objective of the present invention is to provide is a semi auto mated device used for the spreading of a blood drop on the glass slide for the haematological analysis.
Yet another objective of the present invention is to provide an economical, and easy to use automated device for hematological analysis.
Yet another objective of the present invention is to provide an environmental friendly device as there is no power usage required.
Yet another objective of the present invention is that the present invention is portable.
Yet another objective of the present invention is that the present invention helps in obtaining smears of uniform length.
Yet another objective of the present invention is that the present invention prevents the scattering of blood.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example.

SUMMARY OF THE INVENTION
The present invention relates to a semi-automated device for the preparation of blood smear slides includes a housing, a slide holder, a control knob, slide holder a thin metal strip, an at least one spreading slide, and a pair of spreading slide holders. The housing has a front surface, a rear surface, a top surface, a bottom surface and a pair of side surfaces. The rear surface and the front surface are placed opposite to each other. The bottom surface and the top surface are placed opposite to each other. The pair of side surfaces connects the top surface, the bottom surface, the front surface and the rear surface to form the housing. The control knob is placed at the front surface of the housing. The slide holder is placed at the top of the housing, and is configured to hold glass slides. The thin metal strip protrudes outwards from the top surface of the housing. The one end of the pair of spreading side holders is fastened to the thin metal strip and the other end of the pair of spreading slide holders holds the spreading slides. Herein, the front surface and the rear surface are placed longitudinally to the top surface and the bottom surface to form a box-like structure. Herein, the control knob is rotated to moving the slide holder forward and backward. Herein, the slide holder is capable of holding two glass slides, and the slide holder is mounted on the top surface of the housing through a fastening means, and the slide holder is fastened to the thin metal strip. Herein, the at least one spreading slide are placed in between the cavity of spreading slide holders. In an embodiment, the one end of the thin metal strip is fastened to the top surface of the housing and the other end of the thin metal strip is fastened with the pair of spreading side holders. Herein, the slide holder is fastened to the thin metal strip in such a way so as to have an angle in the range of 30°-45° between the slide holder and the pair of spreading slide holders. In an embodiment, the pair of spreading slide holders are capable of moving in a flap- like pattern through the fastening means. In another embodiment, the control knob is configured to control the thickness of the blood smear, i.e by slowly rotating the control knob, a thicker blood smear is obtained.

The present invention relates to a method for the preparation of blood smears slides. The method for spreading of a blood drop on the slide for hematological analysis comprises of a drop of blood placed on the glass slide through a pipette. A slide holder is retracted forward through a control knob and a pair of spreading slide holders are moved manually to spread the blood drop. The control knob is rotated to move the slide holder backward to spread the blood drop evenly on the glass slide. The glass slide having the smeared blood drop is removed from the slide holder and is used for the haematological analysis. In an embodiment, at step 202, the blood drop of 4 mm diameter is placed on the glass slide. At step 204, the blood drop is spread longitudinally to reach the sides of the glass slide and the angle between the slide holder and the pair of spreading slide holders is in the range of 30°-45°. In another embodiment, at step 206, the thickness of the blood smear is controlled through the speed of the rotation of the control knob and the spreading slides are cleaned with an alcohol.
The main advantage of the present invention is that the present invention is a semi auto mated device used for the spreading of a blood drop on the glass slide for the haematological analysis.
Yet another advantage of the present invention is that the present invention is economical, and easy to use.
Yet another advantage of the present invention is that the present invention is environmental friendly as there is no power usage required to operate the device.
Yet another advantage of the present invention is that the present invention is portable.
Yet another advantage of the present invention is that the present invention helps in obtaining smears of uniform length.
Yet another advantage of the present invention is that the present invention prevents the scattering of blood.

Yet another advantage of the present invention is that the present invention prepares two slides at a time.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example.
DETAILED DESCRIPTION OF THE INVENTION
Definition
The terms "a" or "an", as used herein, are defined as one or as more than one. The term "plurality", as used herein, is defined as two as or more than two. The term "another", as used herein, is defined as at least a second or more. The terms "including" and/or "having", as used herein, are defined as comprising (i.e., open language). The term "coupled", as used herein, is defined as connected, although not necessarily directly, and not necessarily mechanically.
The term "comprising" is not intended to limit inventions to only claiming the present invention with such comprising language. Any invention using the term comprising could be separated into one or more claims using "consisting" or "consisting of claim language and is so intended. The term "comprising" is used interchangeably used by the terms "having" or "containing".
Reference throughout this document to "one embodiment", "certain embodiments", "an embodiment", "another embodiment", and "yet another embodiment" or similar terms means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of such phrases or in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics are combined in any suitable manner in one or more embodiments without limitation.

The term "or" as used herein is to be interpreted as an inclusive or meaning any one or any combination. Therefore, "A, B or C" means any of the following: "A; B; C; A and B; A and C; B and C; A, B and C". An exception to this definition will occur only when a combination of elements, functions, steps, or acts are in some way inherently mutually exclusive.
As used herein, the term "one or more" generally refers to, but not limited to, singular as well as the plural form of the term.
As used herein, the term fastening generally refer to fasteners such as, but not limited to nut, bolts, washers, nails, rivets, and screws.
The drawings featured in the figures are to illustrate certain convenient embodiments of the present invention and are not to be considered as a limitation to that. Term "means" preceding a present participle of operation indicates the desired function for which there is one or more embodiments, i.e., one or more methods, devices, or apparatuses for achieving the desired function and that one skilled in the art could select from these or their equivalent in view of the disclosure herein and use of the term "means" is not intended to be limiting.
Figure 1 illustrates a semi auto mated device (100) for the preparation of a glass slide
Figure 2 illustrates the method (200) for the spreading of the blood drop evenly on the glass slide for the haematological analysis.
Figure 1 illustrates the semi-automated device (100) for the spreading of the blood drop evenly on the glass slide for the haematological analysis. The semi-automated device (100) includes a housing (102), a slide holder (118), a control knob (116) and a pair of spreading slide holders (122).
Figure 2 illustrates the method (200) for the spreading of the blood drop evenly on the glass slide for the haematological analysis. The method (200) includes the following steps:

At step 202, the drop of blood is placed on the glass slide through a pipette. At step 204, the slide holder (118) is retracted forward through the control knob (116) and the pair of spreading slide holders (122) are moved manually to spread the blood drop. At step 206, the control knob (116) is rotated to move the slide holder (118) backward to spread the blood drop evenly on the glass slide. At step 208, the glass slide having the smeared blood drop is removed from the slide holder (118) and is used for the haematological analysis.
The present invention relates to a semi-automated device(lOO) for the preparation of blood smear slides includes a housing (102), a slide holder (118), a control knob (116), slide holder (118) a thin metal strip (120), an at least one spreading slide (124), and a pair of spreading slide holders (122). The housing (102) has a front surface (104), a rear surface (106), a top surface (108), a bottom surface (110) and a pair of side surfaces (112,114). The rear surface (106) and the front surface (104) are placed opposite to each other. The bottom surface (110) and the top surface (108) are placed opposite to each other. The pair of side surfaces (112,114) connects the top surface (108), the bottom surface (110), the front surface (104) and the rear surface (106) to form the housing (102). The control knob (116) is placed at the front surface (104) of the housing (102). The slide holder (118) is placed at the top of the housing (102), and is configured to hold glass slides. The thin metal strip (120) protrudes outwards from the top surface (108) of the housing (102). The one end of the pair of spreading side holders (122) is fastened to the thin metal strip (120) and the other end of the pair of spreading slide holders (122) holds the spreading slides (124). Herein, the front surface (104) and the rear surface (106) are placed longitudinally to the top surface (108) and the bottom surface (110) to form a box-like structure. Herein, the control knob (116) is rotated to moving the slide holder (118) forward and backward. Herein, the slide holder (118) is capable of holding two glass slides, and the slide holder (118) is mounted on the top surface (108) of the housing (102) through a fastening means, and the slide holder (118) is fastened to the thin metal strip (120). Herein, the at least one spreading slide (124) are placed in between the cavity of spreading slide holders (122).

In an embodiment, the one end of the thin metal strip (120) is fastened to the top surface (108) of the housing (102) and the other end of the thin metal strip (120) is fastened with the pair of spreading side holders (122). Herein, the slide holder (118) is fastened to the thin metal strip in such a way so as to have an angle in the range of 30°-45° between the slide holder (118) and the pair of spreading slide holders (122).
In an embodiment, the pair of spreading slide holders (122) are capable of moving in a flap- like pattern through the fastening means.
In another embodiment, the control knob (116) is configured to control the thickness of the blood smear, i.e by slowly rotating the control knob (116), a thicker blood smear is obtained.
The present invention relates to a method for the preparation of blood smears slides. The method for spreading of a blood drop on the slide for hematological analysis comprises of a drop of blood placed on the glass slide through a pipette. A slide holder (118) is retracted forward through a control knob (116) and a pair of spreading slide holders (122) are moved manually to spread the blood drop. The control knob (116) is rotated to move the slide holder (118) backward to spread the blood drop evenly on the glass slide. The glass slide having the smeared blood drop is removed from the slide holder (118) and is used for the haematological analysis.
In an embodiment, at step 202, the blood drop of 4 mm diameter is placed on the glass slide. At step 204, the blood drop is spread longitudinally to reach the sides of the glass slide and the angle between the slide holder (118) and the pair of spreading slide holders (122) is in the range of 30°-45°.
In another embodiment, at step 206, the thickness of the blood smear is controlled through the speed of the rotation of the control knob (116) and the spreading slides (124) are cleaned with an alcohol.
The present invention relates to a semi-automated device for the preparation of blood smear slides includes a housing, a slide holder, a control knob, slide holder a thin metal strip, one or more one spreading slide, and a pair of spreading slide

holders. The housing has a front surface, a rear surface, a top surface, a bottom surface and a pair of side surfaces. The rear surface and the front surface are placed opposite to each other. The bottom surface and the top surface are placed opposite to each other. The pair of side surfaces connects the top surface, the bottom surface, the front surface and the rear surface to form the housing. The control knob is placed at the front surface of the housing. The slide holder is placed at the top of the housing, and is configured to hold glass slides. The thin metal strip protrudes outwards from the top surface of the housing. The one end of the pair of spreading side holders is fastened to the thin metal strip and the other end of the pair of spreading slide holders holds the spreading slides. Herein, the front surface and the rear surface are placed longitudinally to the top surface and the bottom surface to form a box-like structure. Herein, the control knob is rotated to moving the slide holder forward and backward. Herein, the slide holder is capable of holding two glass slides, and the slide holder is mounted on the top surface of the housing through a fastening means, and the slide holder is fastened to the thin metal strip. Herein, the at least one spreading slide are placed in between the cavity of spreading slide holders. In an embodiment, the one end of the thin metal strip is fastened to the top surface of the housing and the other end of the thin metal strip is fastened with the pair of spreading side holders. Herein, the slide holder is fastened to the thin metal strip in such a way so as to have an angle in the range of 30°-45° between the slide holder and the pair of spreading slide holders. In an embodiment, the pair of spreading slide holders are capable of moving in a flap- like pattern through the fastening means. In another embodiment, the control knob is configured to control the thickness of the blood smear, i.e by slowly rotating the control knob, a thicker blood smear is obtained.
The present invention relates to a method for the preparation of blood smears slides. The method for spreading of a blood drop on the slide for hematological analysis comprises of a drop of blood placed on the glass slide through a pipette. A slide holder is retracted forward through a control knob and a pair of spreading slide holders are moved manually to spread the blood drop. The control knob is rotated to move the slide holder backward to spread the blood drop evenly on the glass

slide. The glass slide having the smeared blood drop is removed from the slide holder and is used for the haematological analysis. In an embodiment, at step 202, the blood drop of 4 mm diameter is placed on the glass slide. At step 204, the blood drop is spread longitudinally to reach the sides of the glass slide and the angle between the slide holder and the pair of spreading slide holders is in the range of 30°-45°. In another embodiment, at step 206, the thickness of the blood smear is controlled through the speed of the rotation of the control knob and the spreading slides are cleaned with an alcohol.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein, in which various embodiments of the disclosed present invention are illustrated by way of example and appropriate reference to accompanying drawings. Those skilled in the art to which the present invention pertains may make modifications resulting in other embodiments employing principles of the present invention without departing from its spirit or characteristics, particularly upon considering the foregoing teachings. Accordingly, the described embodiments are to be considered in all respects only as illustrative, and not restrictive, and the scope of the present invention is, therefore, indicated by the appended claims rather than by the foregoing description or drawings. Consequently, while the present invention has been described with reference to particular embodiments, modifications of structure, sequence, materials and the like apparent to those skilled in the art still fall within the scope of the invention as claimed by the applicant.

1/ WE CLAIMS
1. A semi-automated device for the preparation of blood smear slides comprises of:
a housing (102), the housing (102) comprises of: a front surface (104);
a rear surface (106), the rear surface (106) and the front surface (104) are placed opposite to each other; atop surface (108);
a bottom surface (110), the bottom surface (110) and the top surface (108) are placed opposite to each other; and a pair of side surface (112,114), the pair of side surfaces (112,114) connects the top surface (108), the bottom surface (110), the front surface (104) and the rear surface (106) to form the housing (102);
a control knob (116), the control knob (116) is placed at the front
surface (104) of the housing (102);
a slide holder (118), the slide holder (118) is placed at the top of the
housing (102), and is configured to hold glass slides;
a thin metal strip (120), the thin metal strip (120) protrudes outwards
from the top surface (108) of the housing (102);
an at least one spreading slide (124); and
a pair of spreading slide holders (122); one end of the pair of
spreading side holders (122) is fastened to the thin metal strip (120)
and the other end of the pair of spreading slide holders (122) holds
the spreading slides (124);
wherein, the front surface (104) and the rear surface (106) are placed longitudinally to the top surface (108) and the bottom surface (110) to form a box-like structure,
wherein, the control knob (116) is rotated to moving the slide holder (118) forward and backward,

wherein, the slide holder (118) is capable of holding two glass slides, and the slide holder (118) is mounted on the top surface (108) of the housing (102) through a fastening means, and the slide holder (118) is fastened to the thin metal strip (120),
wherein, the at least one spreading slide (124) are placed in between the cavity of spreading slide holders (122).
2. The thin metal strip (120) as claimed in claim 1, wherein, the one end of the thin metal strip (120) is fastened to the top surface (108) of the housing (102) and the other end of the thin metal strip (120) is fastened with the pair of spreading side holders (122).
3. The thin metal strip (120) as claimed in claim 1, wherein the slide holder (118) is fastened to the thin metal strip in such a way so as to have an angle in the range of 30°-45° between the slide holder (118) and the pair of spreading slide holders (122).
4. The pair of spreading slide holders (122) as claimed in claim 1, wherein, the pair of spreading slide holders (122) are capable of moving in a flap- like pattern through the fastening means.
5. The control knob (116) as claimed in claim 1, wherein, the control knob (116) is configured to control the thickness of the blood smear, i.e by slowly rotating the control knob (116), a thicker blood smear is obtained.
6. A method for the preparation of blood smears slides, the method for spreading of a blood drop on the slide for hematological analysis comprises of:
a drop of blood placed on the glass slide through a pipette;
a slide holder (118) is retracted forward through a control knob (116)
and a pair of spreading slide holders (122) are moved manually to
spread the blood drop;
the control knob (116) is rotated to move the slide holder (118)
backward to spread the blood drop evenly on the glass slide;

the glass slide having the smeared blood drop is removed from the slide holder (118) and is used for the haematological analysis.
7. The method as claimed in claim 6, wherein, the blood drop is spread longitudinally to reach the sides of the glass slide and the angle between the slide holder (118) and the pair of spreading slide holders (122) is in the range of30°-45°.
8. The method as claimed in claim 6, wherein, the thickness of the blood smear is controlled through the speed of the rotation of the control knob (116).