Description:5. Detailed Description of the Invention:
• 5.1 Materials and Methods: Combine the "Chemicals and Materials" and "Instruments" sections into one.
o List all chemicals and their sources (e.g., Efavirenz, analytical grade, Merck Specialties Pvt Ltd).
o Specify the exact models and manufacturers of the instruments used (e.g., Shimadzu UV-Vis spectrophotometer, model XYZ).
• 5.2 Standard Solution Preparation: Describe the preparation of standard Efavirenz solutions with different known concentrations.
• 5.3 Sample Preparation: Explain the process for extracting and preparing samples from pharmaceutical formulations containing Efavirenz. This may involve steps like crushing tablets, dissolving in suitable solvent, and filtration.
• 5.4 Method 1: Ferric Chloride Complexation:
o Provide a step-by-step procedure for this method, including:
? Volume of standard or sample solution used
? Addition of ferric chloride solution and any necessary solvents/buffers
? Mixing, incubation time, and any filtration steps
? Measurement of absorbance at 520 nm
? Use of a reference blank and explain its preparation
• 5.5 Method 2: 1,10-Phenanthroline Complexation:
o Similarly, describe the step-by-step procedure for this method, including absorbance measurement at 450 nm and reference blank preparation.
• 5.6 Calibration Curve Construction: Explain how to construct a calibration curve using the standard solutions and their absorbance values.
• 5.7 Sample Analysis and Calculations: Describe how to use the calibration curve to calculate the Efavirenz concentration in unknown samples based on their measured absorbance
, C , Claims:Claim 1:
A spectrophotometric method for quantifying Efavirenz in a sample, comprising the steps of:
(a) forming a colored complex between Efavirenz and a complexing agent selected from the group consisting of ferric chloride and 1,10-phenanthroline in a suitable solvent;
(b) measuring the absorbance of the complex at a predetermined wavelength specific to the chosen complexing agent;
(c) constructing a calibration curve by plotting the absorbance of known concentrations of Efavirenz against their corresponding concentrations; and
(d) calculating the concentration of Efavirenz in the sample based on its measured absorbance and the calibration curve.
Claim 2:
The method of claim 1, wherein the complexing agent is ferric chloride and the predetermined wavelength is 520 nm.
Claim 3:
The method of claim 1, wherein the complexing agent is 1,10-phenanthroline and the predetermined wavelength is 450 nm.
Claim 4:
The method of claim 1, wherein the sample is obtained from a pharmaceutical formulation