DESC:FIELD OF THE INVENTION
[001] The invention relates to a synergistic composition comprising at least three active ingredients along with agrochemically acceptable excipients.

BACKGROUND OF THE INVENTION
[002] In the present world the agriculture industry follows a mix and match combination of fertilizers, pesticides and genetically engineered crops for getting high yields for the world. High yielding variety of crops helped us to prevent insect attacks in some cases but pushed the crops to be more prone to these attacks as the new crops lost their natural defence mechanisms. Current farming practices are being greatly challenged with increasing labour shortage, water shortage, demand of high and quality yields, leaching of fertilizers and pesticides and micronutrient deficiencies in the soil etc. The current agriculture practices are also heavily depended on the need to manufacture methods to ward off harmful pests and insects. The inventors also have to keep in mind to attain at a composition which is not toxic to plants and humans who consume them also.
[003] Another problem faced by the farmers since the advent of agriculture is pests resulting in a reduced crop yield, and on occasions, complete crop failure. Over the years, there has been an overuse of fertilizers, fungicides, pesticides and other chemicals. Thus, there is greater need today to optimize farming practices by reducing the number of applications of various fertilizers and pesticides, reduce the
burden on the environment by reducing the number of chemical adjuvants and excipient being added to the soil and the crops, without affecting crop yield.
[004] The main issue farmers face with the use of insecticides is that the repeated and exclusive application of an individual insecticidal compound leads in many cases to develop natural or adapted resistance against the active compound. Inevitably the prerequisite for insecticidal products is that they must be developed such that it prevents or overcomes resistance. But these insecticides, in particular to control different insects, are made up of high dosage rates which are needed to effectively control the disease. Contemporary manufacturing practices use a variety of compounds that were developed using extensive research. These compounds range from fine-tuned ingredients for a special situation to broad spectrum insecticides. However, it should be kept in mind that the cost and focus need to develop such a mixture is significantly higher.
[005] Due to these reasons, it is also common knowledge that the prevailing insecticides used in the market are suffering from the problems of unfavorable environmental/toxicological effects that arises with it. The needs of the agriculture sector have pushed the scientists to develop insecticidal compositions containing more than one active ingredient in mixture to obtain the desired effect. However, all mixtures of ingredients don’t guarantee efficiency as the chemical and physical stability of the product is an essential factor. It is also pertinent to note that the insecticide should not deter the effects of fertilizers and fungicides used alongside. This directly adds to the cost and research needed to develop new insecticides.
[006] While many products are commercially available for this purpose, there is still a need for new mixtures and compositions that are more effective, less expensive, less toxic, more environmentally safe and have various mechanisms of action. The use of multiple active ingredients in a synergistic manner gives users the ability to provide synergistic control for managing the pest/insect attack on the crops without the risk of increased resistance being developed in the target pest population.
SUMMARY OF THE INVENTION
[007] One aspect of the present invention relates to a synergistic composition comprising components:
a. A first active Juvenile hormone mimics in an amount from 0.1wt% to 75wt% of the total weight of the composition;
b. at least one second active selected from a group of inhibitors of mitochondrial ATP synthase and neonicotinoids in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
c. at least one third active is selected from a group of spinosyns, tetronic acid insecticides, and pyrazoles insecticide in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
d. at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
[008] The present invention also relates to a method for synergistic control of insects by contacting the insect or their food supply, habitat, breeding grounds or their locus with a synergistically effective amount of a combination of a) Juvenile hormone mimics insecticide, b) at least one second active selected from inhibitors of mitochondrial ATP synthase and neonicotinoids and c) at least one third active is selected from a spinosyns, tetronic acid insecticides, and pyrazoles insecticide.
[009] Yet another aspect of the present invention is to provide a synergistic composition that achieves increased yield in the crops to which it is applied.
[010] Another aspect of the present invention provides a process for the preparation of a synergistic composition.
[011] Yet another aspect of the present invention is to provide a method of treatment for plants.
[012] The three active ingredients in the compositions of the present invention exhibit synergistic effects and are more effective than the individual activities of the individual compounds.
DESCRIPTION OF THE INVENTION
[013] Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods. An illustrative example is described in this section in connection with the embodiments and methods provided.
[014] It is to be noted that, as used in the specification, the singular forms “a”, “an” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
[015] The expression of various quantities in terms of “%” or “% w/w” means the percentage by weight of the total solution or composition unless otherwise specified.
[016] The present invention is directed towards a synergistic composition for inhibiting insects/pests that damages the plant/crops. The composition shows synergistic effect and enhanced pesticidal activity as compared to single active ingredient against a range of pest and insect infections. The synergistic effect is manifested by allowing for a reduced application rate, a broader insecticidal spectrum, a more durable control effect, better control of the pest by only one or a few applications, and a widening of the possible application intervals. It is also found that a combination of agrochemically acceptable excipients in a pre-determined ratio is helping to improve the physical and chemical stability and prevents the degradation of active ingredients which leads to long term stability and long-range spectrum activity.
[017] In one aspect of the present invention, the composition comprises of:
a. Juvenile hormone mimics;
b. at least one a second insecticide selected from an inhibitor of mitochondrial ATP synthase and Neonicotinoids;
c. at least one third insecticide compound selected from a group of spinosyns, tetronic acid insecticides, and pyrazoles insecticides;
d. at least one agrochemically acceptable excipient.
In an embodiment of the present invention provides a synergistic composition comprising:
a. a first active Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
b. at least one second active selected from a group of inhibitors of mitochondrial ATP synthase and neonicotinoids in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
c. at least one third active is selected from a group of Spinosyns, Tetronic acid insecticides, and Pyrazoles insecticide in an amount from 0.1 wt% to 75 wt% of the total weight of the composition; and
d. at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
[018] In an embodiment of the present invention, the Juvenile hormone mimic/insect growth regulator is selected from, but not limited to, Dayoutong, Epofenonane, Fenoxycarb, Hydroprene, Kinoprene, Methoprene, Pyriproxyfen, Triprene. It mimics natural insect hormones that stop young insects from maturing into adults. The preferred juvenile hormone mimic may be Pyriproxyfen.
[019] In an embodiment of the present invention, the second active is Inhibitors of mitochondrial ATP synthase selected from Azocyclotin, Cyhexatin, Diafenthiuron, Fenbutatin-oxide, Propargite, and Tetradifon and mixtures thereof. The preferred Inhibitors of mitochondrial ATP synthase may be Diafenthiuron. Alternatively, the second active may be Neonicotinoids selected from, but not limited to, Acetamiprid, Clothianidin, Imidacloprid, Imidaclothiz, Nitenpyram, Thiacloprid, Thiamethoxam or Sulfoximines such as sulfoxaflor; Butenolides such as Flupyradifurone, Mesoionics such as Triflumezopyrim and mixtures thereof. The neonicotinoids act on the central nervous system of insects as an agonist of acetylcholine, the neurotransmitter that stimulates nAChR, targeting the same receptor site (AChR) and activating post-synaptic acetylcholine receptors but not inhibiting AChE. On the other hand, nicotinic agonist that reacts with nicotinic acetylcholine receptors (nACh-R). These receptors are located in the post-synaptic dendrites of all neurons in the brain, spinal cord, ganglia and muscular junctions. The activation of the nACh-R receptors causes hyperactivity and muscle spasms, and eventually death.
[020] In an embodiment, the third active ingredient is selected from Spinosyns such as Spinetoram, Spinosad.Tetronic acid insecticides such as Spirodiclofen, Spiromesifen and Pyrazoles selected from Acetoprole, Ethiprole, Fipronil, Flufiprole, Nicofluprole, Pyrafluprole, Pyriprole, Vaniliprole, Dimpropyridaz, Tolfenpyrad or mixtures thereof.
[021] In another embodiment, the total amount of Juvenile hormone mimics in the composition may typically be in the range of 0.1 to 75% by weight, preferably 0.1 to 50% by weight. The total amount of second active in the composition may be in the range of 0.1 to 75% by weight. The total amount of the third active in the composition may be in the range of 0.1 to 75% by weight.
[022] In a first preferred embodiment of the present invention the synergistic agricultural chemical composition includes four components as following:
a. A Juvenile hormone mimics in an amount of from 0.1 wt% to 75 wt% of the total weight of the composition.
b. inhibitors of mitochondrial ATP synthase in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
c. A third active is selected from a group of spinosyns and tetronic acid insecticide in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition.
d. At least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
[023] In a second preferred embodiment of the present invention the synergistic agricultural chemical composition includes four components as following:
a. Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
b. Neonicotinoids in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
c. A third active is selected from a group of spinosyns, tetronic acid insecticides, and pyrazoles insecticide in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition.
d. At least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
[024] In a third preferred embodiment of the present invention the synergistic agricultural chemical composition includes four components as following:
a. a Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
b. Diafenthiuron in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
c. A third active is selected from a group of Spinetoram, Spinosad, Spirodiclofen, Spiromesifen in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition.
d. At least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
[025] In a fourth preferred embodiment of the present invention the synergistic agricultural chemical composition includes four components as following:
a. a Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
b. Neonicotinoids in an amount from 0.1 wt% to 75 wt% of the total weight of the composition.
c. A third active is selected from a group of Spinetoram, Spinosad, Spirodiclofen, Spiromesifen, Acetoprole, Ethiprole, Fipronil, Flufiprole, Nicofluprole, Pyrafluprole, Pyriprole, Vaniliprole, Dimpropyridaz, Tolfenpyrad in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition.
d. At least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
In accordance with an embodiment of the present invention, the agrochemically acceptable excipients are present in an amount in the range of 0.5% to 95% of the total weight of the composition, preferably from 1% to 80% of the total weight of the composition.
[026] In one embodiment, the agrochemically acceptable excipient includes but are not limited to customary formulation adjuvants or components, such as organic solvent, stabilizer, anti-foam, emulsifier, antifreeze agent, preservative, antioxidant, colorant, thickener, dispersing agent, wetting agent, polymeric film forming agent, diluent, inert filler, binder and rheology modifier.
[027] The stabilizing agent is at least one selected from the group, consisting of hexamethylene tetra amine, sodium bi sulphite, phosphoric acid monoesters, phosphoric acid diesters, phosphoric acid mono- and di- ester mixture, sodium phosphates, butylated hydroxytoluene, castor oil ethoxylate, ethoxylated hydrogenated castor oil, vegetable oil and epoxidized soyabean oil or combinations thereof.
[028] The antifoam includes to silicon emulsions, poly dimethyl siloxanes.
[029] The emulsifiers includes Polyoxyethylene alkylamine, Polyoxyethylene linear alkyl ether, Ethoxylated Castor Oil, Epoxidized soyabean oil, Polyoxyethyene alkylamine quaternary, Polyoxyethyene tallow ethylmonium ethosulfate, Glycerol monostearate, Glycerol monooleate, ethoxylated monostearate / distearate / tri stearate ethoxylated monooleate / trioleate, Polyoxyethylene branched tridecyl Phosphate, Polyoxyethylene branched tridecyl phosphate neutralised, Polyoxyethylene alkyl ether phosphate, ethoxylated propoxylated sorbitan mono esters, Tallow amine ethoxylate, phosphoric acid monoesters, phosphoric acid diesters, phosphoric acid mono- and di- ester mixture, sodium phosphates, butylated hydroxytoluene, castor oil ethoxylate, ethoxylated hydrogenated castor oil, vegetable oil and epoxidized soyabean oil or combinations thereof.
[030] The dispersing agent includes, but is not limited to, Anionic graft polymer, Kraft lignin Polymer, Napthalene sulphonate formaldehyde condensate, Amine alkyl benzenesulfonate, Sodium Ligno Sulphonate, Blend of Naphthalene sulphonate sodium salts, Blend of Lignin sulphonates sodium salts, Ethoxylated Lignin sulphonates, Linear alkyl benzene sulphonate, tri stearyl phenol ethoxylates, Acrylic copolymer solution, Modified styrene acrylic polymer, Ammonium distyrylphenyl ether sulphate, salts of polystyrene sulphonic acids.
[031] Wetting agent includes Polyalkylene oxide block copolymer, Salts of Di-octyl sulfosuccinate, Alcohol alkoxylate diester, Sodium N-methyl oleoyl taurate, Sodium cocoyl sarcosinate, sodium lauryl sulphate, Sodium dioctyl sulfosuccinate, alkyl polyglucoside, Naphthalenesulfonic acids, branched and linear Butyl derivatives sodium salts, Sodium Lauryl sulphate.
[032] The antifreeze agents include Propylene glycol, 1 methoxyl 2 propoanol, Butyl cellosolve, ethylene glycol.
[033] The preservatives include, but is not limited to, 1-2-benziisothiazolin-3-one, 5-Chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one.
[034] The rheology modifiers include Polyester block co-polymer, Poly saccharides, Polyamide, Clay, xanthum gum, Silicates.
[035] The binder / film-forming agent is at least one selected from the group consisting of hydrophilic polymers, hydrobhobic polymers, Poly(vinylpyrrolidone), vinypyrrolidone-vinylacetate and the filler, at least one selected from the group consisting of lactose, glucose, fructose, mannose, mannitol, sucrose, and the like, can also act as binding agent.
[036] The disintegrating agent is selected from the group consisting of sodium chloride, sodium sulphate, ammonium sulphate, sodium carbonate, sodium bicarbonate, sodium tripolyphosphate (STPP), Crosslinked carboxyl methyl cellulose, crosslinked PVA, crosslinked starch, crosslinked alginic acid and Ca silicate, etc. Also, hydrocarbons such as lactose, sucrose, dextrose
[037] The solvents, include aromatic hydrocarbon solvent such as SOLVESSO®, paraffinic hydrocarbon solvent such as EXXSOL®, cyclic hydrocarbon solvent such as cyclohexanone, N-methylpyyrolidone, mixed xylene, cyclohexane, Dimethyl sulphoxide, Diemthyl formamide, toluene, ethyl methyl ketone, Tetrahydrofuran, Other solvent / co solvent includes such as N butyl Pyrolliodone, Dimethylamide of C8/C10 fatty acid, Dimethylamide of C10 fatty acid, Dimethyl Amide, Dipropylene Glycol, Caprylic/Capric Triglyceride, Canola Oil Methyl Ester, Methyloleate/Linoleate Methyl Ester, N,N-Dimethyl 9-Dodecenamide, Methyl Caprylate/Caprate, N, N-Dimethyloctanamide (N, N-Dimethylcaprylamide) and N, N-Dimethyl-Decanamide [(N, N-Dimethylcapramide)], alkanes, alkanol, etc.
[038] The Co-solvent includes Bicyclic Tetraether, alkanes, alkenes, Dibasic ester solvents, Alkylene carbonates including Esters, hydrocarbons amides, aldehyde, ketones, ether, esters, Carbonate based, Aromatic Ester solvent, Benzyl Acetate, Dimethyl propylene mono methyl ether, Formylmorpholine, 2-ethylhexyl ester of natural L-Lactic acid ( Ethtyl Hexyl lactate), alcohol, Ethyl acetate, Polyethylene glycol, Chloroform and chlorinated hydrocarbons, etc.
[039] The filler and/or diluent is selected from the group consisting of starches and their derivatives, sugars and sugar alcohols, silicates, calcium phosphates, calcium sulfate, dextrates, kaolin clay, bentonite clay, attapulgite, diatomaceous earth, magnesium carbonate, ammonium sulphate, Calcium sulphate, Magnesium sulphate, Calcium carbonate, polymethacrylates, talc, or salts.
[040] Water is used as a diluent to dilute the active ingredient to a desired concentration. The water used is purified water and selected from the group consisting of deionized water and distilled water.
[041] In another embodiment, the composition is formulated as a formulation selected from water dispersible granules, wettable powder, capsule suspension, soluble (liquid) concentrates or suspension concentrate, oil in water emulsion, water in oil emulsion, oil dispersions, capsule suspension, suspension concentrate, emulsifiable concentrates, suspo-emulsion, water dispersible powder, micro emulsion, dual emulsion, nano emulsion, combination of capsule suspension & suspension concentrate, combination of capsule suspension & oil in emulsion, combination of capsule suspension of suspo emulsion.
[042] The composition of the present disclosure may further comprise one or more excipients. The employment of excipients used in the composition will depend upon the type of formulation and/or the manner in which the formulation is to be applied by the end user.
[043] The formulations can be of any type known in the sector that is suitable for application onto all types of cultures or crops. These formulations, which can be prepared in any manner known by the skilled person, also form part of the invention.
[044] In one embodiment, for optimal results, preventive applications may also be carried out. composition has very advantageous curative, preventive and systemic pesticidal properties for protecting cultivated plan. Having curative activity, the composition can also be applied after the infection, but the treatments will be carried out in the initial or first stages of development of the pestsT. The interval between treatments is adjusted according to the pressure of infection, the development of culture and climatic conditions. The pests which are combated with the composition of the invention include Sucking pest complex (White fly, Thrips, Aphids, Jassids & Mites), Sheath blight, Grain discoloration, False smut, Downy mildew, Anthracnose, Rust, Scab, premature leaf fall, Cercospora leaf spot, Leaf spot , Grey Mildew, Anthracnose, Boll rot, Late blight, Early blight, Soft rots, Purple blotch, Stem rot, Alternaria Blight, Powdery mildew, Blister blight, Powdery mildew, Die-back, Anthracnose, Red Rust, Grey blight, Thielaviopsis rot, Leaf & Fruit rot, Banded sheath blight & Charcoal rot.
[045] Under specific conditions, for example according to the nature of the phytopathogenic fungi or insect to control, a lower dose may offer adequate protection. Certain climatic conditions, resistance or other factors like the nature of the phytopathogenic fungi or damaging insect to be eliminated or the degree of infestation, for example, of the plants with these insects, may require higher doses of combined active ingredients.
[046] The synergistic composition of the present invention has uses for controlling variety of Fungal action on various crops such as Cotton, Brinjal, Okra, Grapes, Apple, Black gram, Banana Ground Nut, Citrus, Ginger, Onion, Potato, Tomato, Cumin, Tea, Chilli, Pome, Mango, Pineapple, Wheat and Maize.
[047] The composition of the present invention provides better crop quality, improved growth and enhanced efficacy.
[048] Advantageously, the composition exhibits increased resistance to pest and disease over individual applications, shows unexpected synergy and greatly enhanced shelf life.
EXAMPLES
[049] The present invention is more particularly described in the following examples that are intended as illustration only, since numerous modifications and variations within the scope of the present invention will be apparent to those skilled in the art. Unless otherwise noted, all parts, percentages and ratios reported in the following examples are on a weight basis, and all reagents used in the examples were obtained or are available from the chemical suppliers.
[050] The following examples illustrate the combination of the synergistic composition, with the basic methodology and versatility of the present invention.
Example 1: Pyriproxyfen 12% + Difenthiuron 30% + Spiromecifen 10% SC
Chemical name Function Composition
Pyriproxyfen @98 Active 12.8
Difenthiuron @98 Active 32
Spiromecifen @96 Active 10.9
Graft block co polymer Polymeric dispersant 4
Naphthalene sulphonates Aqueous dispersant 3
Styrene acrylic co-polymer Wetting agent 3
Glycol Antifreeze agent 6
1-2 benziisothiazoline 3- one solution Preservative 0.1
Silicon emulsion Anti-foaming 0.5
Polysaccharide Rheology modifier 0.16
D I Water Diluent QS
Total 100

Example 2: Pyriproxyfen 6% + Difenthiuron 15% + Spiromecifen 5% SC
Chemical name Function % w/w
Pyriproxyfen @98 Active 6.4
Difenthiuron @98 Active 16
Spiromecifen @96 Active 5.5
Sodium salt of napthalene sulphonate Dispersing agent 3
Salt of Napthalene Sulphonic Formaldehyde condensate Wetting agent 3
Lignosulphonate Dispersing agent 5
Dimethyl siliate Anticaking agent 2
Silicon emulsion Defoamer 0.5
Monosaccharide Disintegrating agent 5
Aluminium Magnesium layered silicate Carrier 26.8
Total 100
Water for dough Extrusion aid 18.0 gram

[051] A process for preparation of the synergistic composition with active ingredients and at least one agrochemical excipient for a suspension concentrate (SC) formulation comprising:
a. Preparing a blend of the active ingredients and a suitable agrochemical excipient to obtain a first pre-mix.
b. The dispersing or wetting agents are dissolved in water, thereby preparing an aqueous system for the first pre-mix.
c. The rheology modifier, antifreeze agent and a small portion of water is mixed in a separate vessel allowing pre-swelling of the rheology modifier.
d. The aqueous system prepared in the second step, is now added with a preservative, an antifoam agent, the first pre-mix including the active ingredients with low shear mixing followed by high shear mixing to produce a homogeneous second pre-mix slurry.
e. The second pre-mix slurry is then subjected to a wet ground in a bead mill to a required particle size (d90) < 10 µm, leading to formation of a concentrated mill base.
f. In a last step, a final homogenous solution is made with addition of the rheology modifier and anti-freeze solution with high shear mixing to produce a final homogeneous solution.
Example 3: Pyriproxyfen 12% + Difenthiuron 30% + Spiromecifen 10% WG
Chemical name Function w/w
Pyriproxyfen @98 Active 12.8
Difenthiuron @98 Active 32
Spiromecifen @96 Active 10.9
Sodium salt of napthalene sulphonate Dispersing agent 4
Salt of Napthalene Sulphonic Formaldehyde condensate Wetting agent 3
Lignosulphonate Dispersing agent 3
Dimethyl siliate Anticaking agent 2
Silicon emulsion Defoamer 0.5
Monosaccharide Disintegrating agent 5
Aluminium Magnesium layered silicate Carrier 26.8
Total 100
Water for dough Extrusion aid 18.0 g

[052] A process for preparation of the synergistic composition with active ingredients and at least one agrochemical excipient for a water dispersable granules (WG) formulation comprising:
a. Preparing a blend of the active ingredients and a suitable agrochemical excipient to obtain a first pre-mix.
b. In a second step, the additional components and the first pre-mix are added to a blender and mix till a uniform blended mixture is obtained.
c. In a third step, the blended formulation is passed through an air jet mill to achieve the desired particle size, preferably d (90) < 10 µm.
d. In a next step, the milled formulation is mixed to ensure even distribution of components in the powder to form a second pre-mix.
e. The second pre-mix is converted to dough, which is then extruded via an extruder and the granules which are received are subjected to a fluid bed drier to get the dried free flowing granules.
f. In the last step, the analysis of the granules takes place.
Example 4: Pyriproxyfen 6% + Clothianidin 5% + Tolfenpyrad 12% EC
Ingredients % w/w
Pyriproxyfen @98 6.14
Clothianidin @99 5.06
Tolfenpyrad @99 12.14
Emulsifier 15
Solvent QS
Co solvent 10
Total 100.00

Example 5: Pyriproxyfen 12% + Acetamiprid 8% + Fipronil 10% EC
Ingredients % w/w
Pyriproxyfen @98 12.27
Acetamipirid @97 8.25
Fipronil @98 10.30
Emulsifier 15
Solvent QS
Co solvent 10
Total 100.00

[053] The process for preparation of an Emulsifiable formulation is as follows:
1. Preparing a blend of the active ingredients with solvents and co-solvents to obtain a first pre-mix.
2. The Emulsifier Ratio Screen for Blank (Emulsifier screening step)
3. Emulsifier addition to first premix ? Performance Check ? Final EC Formulation
4. Final EC Formulation ?Filter ? Analysis ? Packaging
EXPERIMENTAL STUDIES AND DETAIL
[054] The experimental studies were carried out to study the evaluation of the effects of the synergistic composition for bio efficacy and phytotoxicity against sucking pests of cotton. In the experimental studies carried out, the evaluation of Pyriproxyfen, Diafenthuiron and Spiromesifen suspension concentrates (SC) for Bio-efficacy and Phytotoxicity against Sucking pests such as Whitefly, Jassids of Cotton.
[055] The combinations of the insecticides in Example 1 and Example 2:
1. Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC
2. Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC
[056] The objectives of the above experimental studies are:
a. To study the efficacy of the combinations of insecticides (Pyriproxyfen + Diafenthiuron + Spiromesifen SC) against sucking pests of Cotton.
b. To study comparative efficacy of test insecticides with market standards- Pyriproxyfen 10% EC, Diafenthiuron 50%WP & Spiromesifen 22.9%SC and their tank mix combinations.
c. To study the effect of testing insecticides on yield of Cotton.
d. To study the Phytotoxicity effect of test insecticides on Cotton.
[057] Accordingly, to prolong the effectiveness of insecticides liable to encounter resistance problems and to limit crop losses.
[058] The bio efficacy and phytotoxicity treatment details are as following:
1. Bio-efficacy Treatment Details:
Tr. No Treatment Details Dose
(g a.i./ha) Dose
(ml or g/ha)

T1 Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC 60+150+50 500
T2 Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC 30+75+25 500
T3 Pyriproxyfen 10%EC + Diafenthiuron 50%WP+ Spiromesifen 22.9%SC (Tank Mix) 100+300+96 1000+600+400
T4 Pyriproxyfen 10 % EC + Diafenthiuron 50%WP (Tank Mix) 100+300 1000+600
T5 Diafenthiuron 50%WP + Spiromesifen 22.9%SC (Tank Mix) 300+96 600+400
T6 Pyriproxyfen 10%EC + Spiromesifen 22.9%SC (Tank Mix) 100+96 1000+400
T7 Pyriproxyfen 10%EC 100 1000
T8 Diafenthiuron 50%WP 300 600
T9 Spiromesifen 22.9%SC 96 400
T10 Untreated Control NA NA

2. Phytotoxicity Treatment Details:
Tr. No Treatment Details Dose
(g a.i./ha) Dose
(ml or g/ha)

T1 Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC 60+150+50 500
T2 Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC 30+75+25 500
T3 Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC 120+300+100 1000
T4 Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC 60+150+50 1000
T10 Untreated Control NA NA

[059] Further, the materials and methods for the experimental studies to evaluate the bio efficacy and phytotoxicity are discussed as follows:
a) Bio efficacy:
[060] A field experiment was conducted on a farmer’s field during the Kharif in randomized block design with ten treatments and three replications to determine the bio-efficacy of two premix recipes of Pyriproxyfen, Diafenthiuron, and Spiromesifen, namely Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC and Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC.
[061] The premix recipes of Pyriproxyfen, Diafenthiuron and Spiromesifen were compared for bio efficacy with
i. One three-way tank mix combination, i.e. Pyriproxyfen 10%EC + Diafenthiuron 50%WP+ Spiromesifen 22.9% SC.
ii. Three two-way tank mix combinations i.e. Pyriproxyfen 10 % EC + Diafenthiuron 50%WP, Diafenthiuron 50%WP + Spiromesifen 22.9%SC, Pyriproxyfen 10%EC + Spiromesifen 22.9%SC.
iii. Three solo insecticides (Pyriproxyfen 10%EC, Diafenthiuron 50%WP, Spiromesifen 22.9% SC) and an untreated control against Jassid and Whitefly.
[062] The premix recipes of Pyriproxyfen, Diafenthiuron and Spiromesifen were compared for bio efficacy with:
i. One three-way tank mix combination, i.e. Pyriproxyfen 10%EC + Diafenthiuron 50%WP+ Spiromesifen 22.9% SC.
ii. Three two-way tank mix combinations i.e. Pyriproxyfen 10 % EC + Diafenthiuron 50%WP, Diafenthiuron 50%WP + Spiromesifen 22.9%SC, Pyriproxyfen 10%EC + Spiromesifen 22.9%SC.
iii. Three solo insecticides (Pyriproxyfen 10%EC, Diafenthiuron 50%WP, Spiromesifen 22.9% SC) and an untreated control against Jassid and Whitefly.
[063] The treatment’s wise foliar spray was applied on cotton at 15 days interval, when pest reached to Economical Threshold Level (ETL) using Knapsack Sprayer fitted with hollow cone nozzle.
[064] A day prior of the initiation of the experiment, the pest incidence was recorded, and subsequent observations were recorded after 7, and 15 days of each spray. The Whitefly and Jassids were counted on 5 pre-tagged plants per plot as 6 leaves selected randomly / plant (2 leaves each from top, middle and bottom). The Mean value were derived for the treatment and used for Statistical analysis of the data.
b. Yield:
[065] The cotton balls from each net plot were individually harvested and weighed. Three pickings were collected, and after the final picking, the total yield from each net plot was calculated and converted to hectare basis (quintal/ha). Subsequently, the data was subjected to statistical analysis.
c. Phytotoxicity:
[066] The Phytotoxicity observations were recorded to assess the damage caused to plants by applying different treatments. The different phytotoxic symptoms such as leaf injury on tips and leaf surface, wilting, vein clearing, necrosis, epinasty and hyponasty were recorded in each treatment. A total of ten plants per plot were assessed, and the observations were recorded before the application of treatments and on the 1st, 3rd, 5th, 7th, and 10th day after the applications. The following Phytotoxicity Rating Scale (0-10) was used to assess the crop injury.
Phytotoxicity Rating Scale (PRS)
Crop Response/Crop Injury Rating
0-100 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100 10

b) Statistical Analysis: Analysis of variance was calculated by using MS-Excel Computer PrograThe statistical studies, analysis for bio efficacy and phytotoxicity of different Insecticides treatments against Jassids of Cotton (Table 1) and Whitefly of Cotton (Table 2) are mentioned below:
Table 1: Bio-efficacy of different Insecticides treatments against Jassids of Cotton
Tr. No. Treatments Dose Jassids / Leaf
g a.i/ha Formulation Ist APPLICATION IInd APPLICATION
g or ml/ha 0
DAA 1
DAA 7
DAA 15
DAA 1
DAA 7
DAA 15
DAA 21
DAA
T1 Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC 60+150+50 500 9.73
(18.2) 3.67
(11.0) 0.6
(4.3) 3.0
(10.0) 1.93
(7.9) 0.6
(4.4) 2.73
(9.4) 3.67
(11.0)
T2 Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC 30+75+25 500 9.47
(17.9) 5.4
(13.4) 1.0
(4.0) 3.73
(11.0) 3.47
(10.6) 1.2
(6.2) 3.6
(10.9) 5
(12.9)
T3 Pyriproxyfen 10%EC + Diafenthiuron 50%WP+ Spiromesifen 22.9%SC (Tank Mix) 100+300+96 1000+600+400 10.33
(18.7) 6.73
(15.0) 5.73
(13.7) 7.53
(15.9) 6.73
(15.0) 3.07
(10.1) 5.53
(13.6) 7.13
(15.5)
T4 Pyriproxyfen + Diafenthiuron (Tank Mix) 100+300 1000+600 10.27
(18.7) 9.0
(17.4) 8.27
(16.7) 9.53
(18.0) 8.2
(16.6) 4.0
(11.5) 5.0
(12.9) 5.53
(13.6)
T5 Diafenthiuron 50%WP + Spiromesifen 22.9%SC (Tank Mix) 300+96 600+400 10.8
(19.2) 7.93
(16.3) 7.27
(15.6) 9.0
(17.4) 7.27
(15.6) 3.4
(10.6) 4.53
(12.3) 6.07
(14.3)
T6 Pyriproxyfen 10%EC + Spiromesifen 22.9%SC (Tank Mix) 100+96 1000+400 9.87
(18.3) 8.73
(17.2) 7.73
(16.1) 10.67
(19.1) 9.47
(17.9) 4.93
(12.7) 5.53
(13.6) 7.13
(15.5)
T7 Pyriproxyfen 10%EC 100 1000 9.93
(18.4) 9.93
(18.4) 9.53
(18.0) 10.87
(19.2) 9.67
(18.1) 7.13
(15.5) 8.87
(17.3) 9.8
(18.2)
T8 Diafenthiuron 50%WP 300 600 9.80
(18.2) 8.60
(17.0) 9.07
(17.5) 10.33
(18.7) 8.0
(16.4) 6.93
(15.2) 7.6
(16.0) 8.53
(17.0)
T9 Spiromesifen 22.9%SC 96 400 9.33
(17.8) 8.6.
(17.0) 7.2
(15.5) 8.73
(17.2) 7.87
(16.3) 4.67
(12.4) 6.4
(14.6) 7.53
(15.9)
T10 Untreated Control NA NA 10.27
(18.7) 10.6
(19.0) 12.47
(20.7) 13.0
(21.1) 12.6
(20.8) 13.93
(21.9) 14.73
(22.6) 15.67
(23.3)
CD (0.05) NS 1.73 2.24 1.35 1.60 2.10 1.60 1.20

Figures in parenthesis are arcsine transformed values.
DAA- Days After Application; NS- Nonsignificant
e) Results and Discussion:
i. Bio efficacy: Jassids
[068] The results revealed that, before foliar application of the insecticides, Jassids count per leaf ranged from 9.3 to 10.8 across all treatments and were statistically non-significant. At 7 days after first application, T1 (Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC @ 500 ml/ha) proved to be the most effective (0.60 /leaf) among the insecticides evaluated and it was at par with T2 (Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC @ 500 ml/ha) (Table 1). These two treatments were significantly superior over the rest of the treatments. Significantly highest Jassid population was observed in the untreated check plot (12.4/leaf).
[069] Similarly at 15 days after first application also, significantly lowest Jassids population (3.0/leaf) was observed in treatment T1 followed by T2 (3.73/leaf). These two treatments were on par each other and significantly superior over rest of the treatments against the Jassids. Significantly highest Jassids population was observed in untreated check plot (13.0/leaf).
[070] 7 days after the second application, T1 was recorded as the lowest number of Jassids population (0.6 /Leaf) followed by T2 (1.2/leaf) and these treatments were statistically non-significant. The highest Jassids population was observed in untreated control (13.93 /leaf). A similar trend was observed 15 days after the second application.
[071] 21 days after the second application also similar trend was observed. The significant lowest Jassids were observed in treatment T1 (3.67 /leaf) followed by T2 (5.0 /leaf) and these two treatments were superior to rest of the treatments. The highest population of Jassids was observed in untreated control (15.67/leaf).
Table 2: Bio-efficacy of different Insecticides treatments against Whitefly of cotton
Tr. No. Treatments Dose Whitefly / Leaf
g a.i/ha Formulation Ist APPLICATION IInd APPLICATION
g or ml/ha 0
DAA 1
DAA 7
DAA 15
DAA 1
DAA 7
DAA 15
DAA 21
DAA
T1 Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC 60+150+50 500 16.67
(24.1) 6.67
(14.9) 2.27
(8.7) 3.27
(10.4) 1.4
(6.6) 0.87
(4.1) 2.27
(8.7) 16.67
(24.1)
T2 Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC 30+75+25 500 16.93
(24.3) 8.0
(16.3) 2.8
(9.4) 4.33
(12.0) 2.8
(9.4) 2.27
(8.7) 2.8
(9.4) 16.93
(24.3)
T3 Pyriproxyfen 10%EC + Diafenthiuron 50%WP+ Spiromesifen 22.9%SC (Tank Mix) 100+300+96 1000+600+400 17.67
(24.9) 10.2
(18.6) 8.0
(16.4) 9.07
(17.5) 6.73
(15.0) 6.07
(14.2) 7.67
(16.0) 17.67
(24.9)
T4 Pyriproxyfen + Diafenthiuron (Tank Mix) 100+300 1000+600 17.07
(24.4) 11.27
(19.6) 9.27
(17.7) 10.8
(19.2) 9.07
(17.5) 6.73
(15.0) 7.73
(16) 17.07
(24.4)
T5 Diafenthiuron 50%WP + Spiromesifen 22.9%SC (Tank Mix) 300+96 600+400 17.53
(24.8) 11.6
(19.9) 11.07
(19.4) 11.87
(20.1) 10.33
(18.7) 8.27
(16.7) 11.47
(19.8) 17.53
(24.8)
T6 Pyriproxyfen 10%EC + Spiromesifen 22.9%SC (Tank Mix) 100+96 1000+400 18.8
(25.7) 11.67
(19.9) 10.33
(18.7) 11.07
(19.4) 9.27
(17.7) 7.47
(15.8) 8.87
(17.2) 18.8
(25.7)
T7 Pyriproxyfen 10%EC 100 1000 17.67
(24.9) 13.4
(21.5) 11.27
(19.6) 12.93
(21.0) 11.47
(19.8) 10.27
(18.7) 11.73
(20.0) 17.67
(24.9)
T8 Diafenthiuron 50%WP 300 600 17.8
(24.9) 14.73
(22.5) 13.4
(21.5) 13.6
(21.6) 11.73
(20.0) 10.13
(18.6) 12.87
(21.0) 17.8
(24.9)
T9 Spiromesifen 22.9%SC 96 400 17.67
(24.9) 15.67
(23.3) 15.0
(22.8) 15.67
(23.3) 13.6
(21.6) 11.73
(20) 13.2
(21.3) 17.67
(24.9)
T10 Untreated Control NA NA 16.13
(23.7) 17.67
(24.9) 21.93
(27.9) 23.93
(29.3) 26.07
(30.7) 27.07
(31.3) 27.8
(31.8) 16.13
(23.7)
CD (0.05) NS 2.38 1.61 2.51 2.50 1.80 2.40 2.80

Figures in parenthesis are arcsine transformed values.
DAA- Days after application; NS- Nonsignificant
ii. Bio efficacy: Whitefly
[072] The findings indicated that, prior to the foliar application of insecticides, the Whitefly count per leaf varied from 16.1 to 18.8 among all treatments and were statistically non-significant. However, at 7 days after first application, T1 (Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC @ 500 ml/ha) recorded the lowest whitefly population (2.27/leaf) among the evaluated insecticides, and it was on par with T2 (Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC @ 500 ml/ha) (Table 2). These two treatments were significantly superior over the rest of the treatments. Notably, the untreated check plot exhibited the highest Whitefly population, with a count of 21.9 per leaf.
[073] Similarly, 15 days after first application, treatment T1 exhibited the significantly lowest Whitefly population (3.27/leaf), followed by T2 (4.33/leaf). These two treatments showed similar efficacy and were significantly superior over other treatments in controlling Whitefly incidence. The untreated control plot, on the other hand, recorded the highest Whitefly population, with a count of 23.93 per leaf.
[074] 7 days after second application, T1 was recorded as the lowest Whitefly population (0.87 /Leaf) followed by T2 (2.27/leaf) and these treatments were statistically non-significant. The highest whitefly population was observed in untreated control (27.07 /leaf). This trend remained consistent in the 15 days after the second application.
[075] The trend remained consistent at 21 days after the second application. The significant lowest whitefly population was observed in treatment T1 (2.67 /leaf) followed by T2 (5.0 /leaf) and these two treatments were superior to rest of the treatments. The highest population of whitefly was observed in untreated control (29.67/leaf).
Table 3: Effect of different Insecticides treatments on Cotton Yield
Tr. No Treatment Details Dose (g a.i./ha) Dose (ml or g/ha) Yield (Tones/ha)

T1 Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC 60+150+50 500 617.7
T2 Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC 30+75+25 500 604.0
T3 Pyriproxyfen 10%EC + Diafenthiuron 50%WP+ Spiromesifen 22.9%SC (Tank Mix) 100+300+96 1000+600+400 598.3
T4 Pyriproxyfen + Diafenthiuron (Tank Mix) 100+300 1000+600 583.3
T5 Diafenthiuron 50%WP + Spiromesifen 22.9%SC (Tank Mix) 300+96 600+400 575.0
T6 Pyriproxyfen 10%EC + Spiromesifen 22.9%SC (Tank Mix) 100+96 1000+400 584.7
T7 Pyriproxyfen 10%EC 100 1000 570.0
T8 Diafenthiuron 50%WP 300 600 541.7
T9 Spiromesifen 22.9%SC 96 400 565.0
T10 Untreated Control NA NA 513.3
CD (P = 0.05) 14.1

iii. Yield:
[076] All treatments led to a significant increase in yield compared to the untreated Control (513.3 kg/ha) (Table 3). The highest yield was observed in T1 (617.7 kg/ha), which was statistically on par with T2 (604.0 kg/ha). These two treatments were significantly superior over the remaining three-way, two-way tank mix and solo insecticide treatments.
Table 4: Phyto-toxicity effect of different Insecticide treatments on cotton
Tr. No. Treatment Details
Dose *Phytotoxicity
(Based on 0-10 Phytotoxicity Rating Scale)
g a.i./ha ml or g/ha Before Spray Days after application (DAA)
1 3 5 7 10
T1 Pyriproxyfen 12% + Diafenthiuron 30% + Spiromesifen 10% SC 60+150+50 500 0 0 0 0 0 0
T2 Pyriproxyfen 6% + Diafenthiuron 15% + Spiromesifen 5% SC 30+75+25 500 0 0 0 0 0 0
T3 Pyriproxyfen 10%EC + Diafenthiuron 50%WP+ Spiromesifen 22.9%SC (Tank Mix) 100+300+96 1000+600+400 0 0 0 0 0 0
T4 Pyriproxyfen + Diafenthiuron (Tank Mix) 100+300 1000+600 0 0 0 0 0 0
T5 Diafenthiuron 50%WP + Spiromesifen 22.9%SC (Tank Mix) 300+96 600+400 0 0 0 0 0 0
T6 Pyriproxyfen 10%EC + Spiromesifen 22.9%SC (Tank Mix) 100+96 1000+400 0 0 0 0 0 0
T7 Pyriproxyfen 10%EC 100 1000 0 0 0 0 0 0
T8 Diafenthiuron 50%WP 300 600 0 0 0 0 0 0
T9 Spiromesifen 22.9%SC 96 400 0 0 0 0 0 0
T10 Untreated Control NA NA 0 0 0 0 0 0

*For phytotoxic symptoms- Leaf injury on tips and Leaf surface, Wilting, Vein Clearing, Necrosis, Epinasty and Hyponasty
iv. Phytotoxicity:
[077] The observations on phytotoxicity parameters were recorded before spray and at 3, 5, 7 and 10 days after application. The studies on phytotoxicity indicated that there were no symptoms of phytotoxicity viz., leaf injury on tips/surface, vein clearing, necrosis, hyponasty and epinasty on the Cotton crop at X and 2X dosages (Table 4).
[078] After conducting the studies and experiments for evaluation of Bio efficacy and Phytotoxicity of the synergistic combination on Jassids and Whitefly on Cotton (Gossypium hirsutum) by ready premixes of Pyriproxyfen, Diafenthiuron and Spiromesifen the following conclusions were derived.
[079] Conclusions:
1. The two readymade premix recipes of Pyriproxyfen, Diafenthiuron, and Spiromesifen (T1 & T2) are more effective than remaining three-way, two-way tank mix and solo insecticides in controlling sucking pest complex in cotton.
2. The two premix recipes of Pyriproxyfen, Diafenthiuron, and Spiromesifen (T1 & T2) were found to be non-phytotoxic and safe to cotton crop.
3. The test insecticide combinations produced higher cotton yield.
4. Based on the findings, the test insecticides show synergistic effect for controlling the sucking pests of cotton. The test insecticides can be used effectively and safely for the management of sucking pest complex in cotton than other three-way, two-way tank mix combinations.
The foregoing description of the invention has been set merely to illustrate the invention and is not intended to be limiting. Since the modifications of the disclosed embodiments incorporating the spirit and substance of the invention may occur to the person skilled in the art, the invention should be construed to include everything within the scope of the disclosure.
,CLAIMS:We Claim:
1. A synergistic composition comprising:
a) a first active Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
b) at least one second active selected from a group of inhibitors of mitochondrial ATP synthase and neonicotinoids in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
c) at least one third active is selected from a group of Spinosyns, Tetronic acid insecticides, and Pyrazoles insecticide in an amount from 0.1 wt% to 75 wt% of the total weight of the composition; and
d) at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
2. The synergistic composition as claimed in claim 1, wherein the Juvenile hormone mimics is selected from Pyriproxyfen, Dayoutong, Epofenonane, Fenoxycarb, Hydroprene, Kinoprene, Methoprene and Triprene.
3. The synergistic composition as claimed in claim 1, wherein at least one second active selected from the inhibitor of mitochondrial ATP synthase selected from Diafenthiuron, Azocyclotin, Cyhexatin, Fenbutatin-Oxide, Propargite, and Tetradifon and the neonicotinoids selected from Acetamiprid, Clothianidin, Imidacloprid, Imidaclothiz, Nitenpyram, Thiacloprid, Thiamethoxam, sulfoxaflor, Flupyradifurone, Triflumezopyrim or mixtures thereof.
4. The synergistic composition as claimed in claim 1, wherein the third active ingredient is selected from Spinosyns such as Spinetoram, Spinosad or mixtures thereof; or
Tetronic acid insecticides such as Spirodiclofen, Spiromesifen, or mixtures thereof; or
Pyrazoles selected from Acetoprole, Ethiprole, Fipronil, Flufiprole, Nicofluprole, Pyrafluprole, Pyriprole, Vaniliprole, Dimpropyridaz, Tolfenpyrad and mixtures thereof.
5. The synergistic composition as claimed in claim 1, wherein the agrochemically acceptable excipient selected from organic solvent, stabilizer, anti-foam, emulsifier, antifreeze agent, anticaking agent, carrier, preservative, antioxidant, colorant, thickener, dispersing agent, wetting agent, polymeric film forming agent, diluent, inert filler, binder, rheology modifier and solvent.
6. The synergistic composition as claimed in claim 1, wherein
a. Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
b. Inhibitors of mitochondrial ATP synthase in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
c. at least one third active is selected from a group of spinosyns and tetronic acid insecticide in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition; and
d. at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
7. The synergistic composition as claimed in claim 1, wherein
a. Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
b. Neonicotinoids in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
c. at least one third active is selected from a group of spinosyns, tetronic acid insecticides, and pyrazoles insecticide in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition; and
d. at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
8. The synergistic composition as claimed in claim 1, wherein
a. Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
b. Diafenthiuron in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
c. at least one third active is selected from a group of Spinetoram, Spinosad, Spirodiclofen, Spiromesifen in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition; and
d. at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
9. The synergistic composition as claimed in claim 1, wherein
a. Juvenile hormone mimics in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
b. Neonicotinoids in an amount from 0.1 wt% to 75 wt% of the total weight of the composition;
c. at least one third active is selected from a group of spinetoram, spinosad, spirodiclofen, spiromesifen, acetoprole, ethiprole, fipronil, flufiprole, nicofluprole, pyrafluprole, pyriprole, vaniliprole, dimpropyridaz, tolfenpyrad in an amount from 0.1 wt% to 75 wt% of the total weight of the synergistic composition; and
d. at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 95% of the total weight of the composition.
10. The synergistic composition as claimed in claim 1, wherein the composition is formulated as a formulation selected from water dispersible granule, wettable powder, soluble (liquid) concentrates, suspension concentrates, oil in water emulsion, water in oil emulsion, oil dispersions, capsule suspension, suspension concentrate, emulsifiable concentrates, suspoemulsions and water dispersible powder.
11. A method for synergistic control of insects by contacting the insect or their food supply, habitat, breeding grounds or their locus with a synergistically effective amount of a composition as claimed in claim 1.

Dated this 2nd day of August 2022
Indofil Industries Limited
By their Agent & Attorney

(Nisha Austine)
of Khaitan & Co
Reg. No. IN/PA-1390