DESC:FIELD OF THE INVENTION
The present invention relates to synergistic fungicidal compositions comprising amounts of (A) an fungicidal Azoxystrobin; (B) an fungicidal Tebuconazole; (C) an fungicidal Isoprothiolane. The present invention further relates to process of preparing said composition along with at least one inactive excipients and formulation thereof.
BACKGROUND OF THE INVENTION
Fungicides are chemical compounds used to prevent, control or eradicate the spread of fungi on crop or plants in the gardens or in nursery, ornamental plants, etc. This can cause serious damage resulting in the loss of yield and thus quality as well as profit from the crop plants. Fungicides are also used to fight fungal infections. Fungicides can either be contact or systemic fungicides. A contact fungicide kills fungi when sprayed on the infected its surface; and a systemic fungicide has to be absorbed by the plant.
Various compositions have been developed to control fungi. Practical agricultural experience has shown that the repeated and exclusive application of an individual active compound for the control of harmful fungi in many cases leads to rapid development of those strains which have developed natural or adapted resistance against the active compound in question. Effective control of these fungi with the active compound in question is very difficult in such cases. To reduce the risk of development of resistant strains, mixtures of different active compounds are nowadays conventionally employed for controlling harmful fungus or pests. It is possible to ensure successful control over a relatively long period of time by combining active compounds having different mechanisms of action. Most crop and ornamental plants are subject to attack by several fungi. Damage due to plant diseases to ornamentals, vegetable, field, cereal and fruit crops can cause significant reduction in productivity and thereby result in increased costs to the consumer. Combinations of fungicides are often used to facilitate disease control, to broaden Spectrum of control and to retard resistance development. Accordingly, new Advantageous combinations are needed to provide a variety of options to best Satisfy particular plant disease control needs. Furthermore, certain rare Combinations of fungicides demonstrate a greater-than-additive (i.e., synergistic) effect to provide commercially important levels of plant disease control. When two or more substances in combination demonstrate unexpectedly high biological activity, for example fungicidal activity, the resultant phenomenon may be referred to as synergism. When two or more substances in combination 10 demonstrate unexpectedly high biological activity, for example fungicidal activity, the resultant phenomenon may be referred to as synergism.
Azoxystrobin is a broad spectrum systemic fungicide widely used in agriculture to protect crops from fungal diseases. It was first marketed in 1996 using the brand name Amistar and by 1999 it had been registered in 48 countries on more than 50 crops. It work on the Respiratory system of the fungus, thus depriving the fungus of the energy required to grow, thus effectively killing the fungus
Its IUPAC NAME: Methyl (2E)-2-(2-{[6-(2-cyanophenoxy)pyrimidin-4-yl]oxy}phenyl)-3-methoxyprop-2-enoate and having chemical structure as:

Tebuconazole
Tebuconazole is a systemic broad spectrum fungicide with protective, curative and eradicant action; rapidly absorbed into the vegetative parts of the plant, with translocation principally acropetally. This triazole fungicide used as a seed dressing and foliar spray to control a wide range of diseases such as rusts, smuts, bunt, powdery mildew, leaf spots, and blights.
Its IUPAC NAME RS)- 1-(4-Chlorophenyl)- 4,4-dimethyl-3-(1H, 1,2,4-triazol-1-ylmethyl)pentan- 3-ol and having chemical structure as:

Isoprothiolane
Isoprothiolane is a fungicide used on rice crops. Isoprothiolane belongs to the family of dicarboxylic acids and derivatives, organic compounds containing two carboxylic acid groups, which act by inhibition of phospholipid biosynthesis. It is a systemic fungicide with curative and protective action. The chemical is absorbed by the leaves and roots and translocate acropetally and basipetally
Its IUPAC name Diisopropyl 2-(1,3-dithiolan-2-ylidene)malonateand having chemical structure as:

The present invention thus relates to a defined combination of pesticidal active ingredients, and compositions thereof, and methods for using such combinations in the control or prevention of fungal infections in agriculture.

OBJECT OF THE INVENTION
It is an object of the present invention to provide a novel, synergistic, broad spectrum fungicidal combination of Azoxystrobin, tebuconazole and Isoprothiolane for effective control of sheath blight, Blast and Brown leaf spot diseases in agricultural crops, particularly Rice crop.

SUMMARY OF THE INVENTION
The present invention discloses a composition that comprises a first active ingredient Azoxystrobin, a second active ingredient tebuconazole and a third active ingredient Isoprothiolane along with agriculturally acceptable excipients. The pesticidal combination of present invention is useful in controlling fungal pests, particularly in Rice and other agricultural crops.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT(S):
Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods or examples.
All technical and scientific terms used herein have the same meanings as commonly understood by someone ordinarily skilled in the art to which the present subject matter belongs.
The terminology used, in the present disclosure, is only for the purpose of explaining a particular embodiment and such terminology shall not be considered to limit the scope of the present disclosure.
As used in the present disclosure, the forms "a,” "an," and "the" may be intended to include the plural forms as well, unless the context clearly suggests otherwise. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
The terms "comprises," "comprising," “including,” and “having,” are open ended transitional phrases and therefore specify the presence of stated features, integers, steps, operations, elements, but do not forbid the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. The particular order of steps disclosed in the method and process of the present disclosure is not to be construed as necessarily requiring their performance as described or illustrated. It is also to be understood that additional or alternative steps may be employed.
Variations or modifications to the composition of this invention, within the scope of the invention, may occur to those skilled in the art upon reviewing the disclosure herein. Such variations or modifications are well within the spirit of this invention.
The expression of various quantities in terms of “% w/w” or “%” means the percentage by weight, relative to the weight of the total formulation or composition unless otherwise specified.
The term “active ingredient” (a.i.) or “active agent” or “active” used herein refers to that component of the composition responsible for control and killing of insect pests.
The term “synergistic”, as used herein, refers to the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.
The term “crop” shall include a multitude of desired crop plants or an individual crop plant growing at a locus.
The term “control” means to inhibit the ability of insect pests to survive, grow, feed and/or reproduce, or to limit the pests related damage or loss in crop plants.
The term “pests” used herein includes insect pests affecting the crops.
The term “health of a plant” or “plant health” is defined as a condition of the plant and/or its products.
As a result of the improved health; yield, plant vigour, quality and tolerance to abiotic or biotic stress is increased. One indicator for the condition of the plant is the yield.
“Yield” is to be understood as any plant product of economic value that is produced by the plant such as grains, fruits, vegetables, nuts, grains, seeds, wood (e.g. in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals). The plant products may in addition be further utilized and/or processed after harvesting.
In an especially preferred embodiment of the invention, the yield of the treated plant is increased.
In another preferred embodiment of the invention, the yield of the plants treated using the composition of the present invention, is increased synergistically.
The term "fungicidal" also called antimycotic, any toxic substance used to kill or
inhibit the growth of fungi.
The terms “weight percent”, “wt-%”, “percent by weight”, “% by weight” andvariations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the composition andmultiplied by 100. It is understood that, as used here, “percent “, “%” and the likeare intended to be synonymous with “weight percent”, “wt. %”, etc.
As used herein, the term “agriculturally acceptable excipient” refers to aningredient, additive, component or supplement, liquid or solid, suitable for incorporation in agricultural compositions.
The present invention provides a fungicidal composition comprising a) Azoxystrobin as the first active ingredient b) tebuconazole as the secondactive ingredient and c) Isoprothiolane as the third active ingredient, wherein
Azoxystrobin is present in an amount ranging from 1-20% w/w, Tebuconazole is present in an amount ranging from 1-25 % w/w, Isoprothiolane ispresent in an amount ranging from 1-25% w/w.
In a preferred embodiment, Azoxystrobin is present in an amount of 7.5%% w/w, tebuconazole is present in an amount of 12% w/w isoprothiolane is present in an amount of 12% w/w of thefungicidal composition.
In an embodiment, the fungicidal composition of the present invention comprisingthe said actives exhibits synergistic effect in control of fungal in agricultural crops, particularly in Rice crop.
Surprisingly, the present inventors have now found that the presently claimedfungicidal composition when applied at significantly lesser dosages thanrecommended dosages when applied separately, exhibit superior efficacy asmeasured in terms of percent control of target pest. In other words, it wassurprising to find that these conventional rice fungicides when combined atdesired weight percentages at reduced individual AI (Active Ingredient) dosages, the resulting efficacy in controlling sheath blight, Blast and Brown leaf spot diseases in Rice is unexpectedly enhanced.
Further surprisingly, it has been found that the active compound combination of the presently claimed composition exhibits a synergistic effect and not merely an additive effect. Thus, clearly, the customary dosages of the individual actives are substantially reduced, leading to lesser amount of soil residues and environmental pollution when the composition of the present invention is used.
In an embodiment, the fungicidal composition further comprises at least one agriculturally acceptable excipient. In an embodiment, the at least one excipient is selected from the group consisting of at least a wetting agent, at least a dispersing agent, at least a defoaming agent, at least a binder, at least a suitable carrier, and combinations thereof.
In a preferred embodiment, the fungicidal composition is in the form of a suspension Emulsion formulation further comprising at least a wetting agent, at least a dispersing agent, at least a defoaming agent, at least a rheology modifiers, at least a biocide ,at least a solvent and atleast carrier.
In an embodiment, the at least a dispersing agent is selected from the group consisting of sodium lignosulphonates, sodium naphthalene sulphonateformaldehyde condensates, aliphatic alcohol ethoxylates, tristyrylphenolethoxylates and esters, ethylene oxide/propylene oxide block copolymers, polymeric dispersant and combinations thereof.
In a preferred embodiment, the at least a dispersing agent is polymeric dispersantThe at least a dispersing agent weight concentration in said fungicidal composition is in the range of 6 to 10 %w/w. In a preferred embodiment, the dispersing agent is present in an amount of 8% w/w.
In an embodiment, the at least a wetting agent is selected from the group consisting of blend of alkyl naphthalene sulfonate, sodium salt, sodium laurel sulphate, polymeric co-dispersant and combinations thereof. In a preferred embodiment, the at least a wetting agent is polymeric co-dispersant.
The at least a wetting agent weight concentration in said fungicidal composition is in the range of 2 to 5% w/w. In a preferred embodiment, the wetting agent is present in an amount of 3% w/w.
In an embodiment, the at least an defoaming is selected from the group consisting of silicone emulsions, long-chain alcohols, fatty acids, organic fluorine compounds, and combinations thereof.
The at least an defoaming agent weight concentration in said fungicidal composition is in the range of 0.1 to .5% w/w. In a preferred embodiment, the antifoaming agent is present in an amount of 0.2% w/w.
In an embodiment , the atleasta rheology modifier is selected from the group consisting of clay, fumed silica, cellulosics, acrylics,carbopol,speacility clays , xanthum gum and combinations thereof.
The at least a rheology modifier is said fungicidal composition is in the range of 0.1-0.5 % w/w. in prefereedembodiement , the rheology modifiers agent is present in an amount of 0.2%
In an embodiment , the atleast a biocide is selected from the group consisiting of sodium benzoate, 1,2- benzisothiazoline-3-one, 2-5 methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4- isothiazolin-3-one, potassium sorbate, para hydroxy benzoates or mixtures thereof.In a preferred embodiment , the biocide agent is present in an fungicidal composition is Benzisothiazolinone.
The atleast a biocide agent weight concentration in said fungicidal composition is in range of 0.1-0.5 %. In a preferred embodiement , the biocide agent is present in an amout of 0.2%.
In an embodiment , the atleast solvent is selected from the group consisiting of Sovent C-9, Methanol, ethanol, toluene,water, and acetone.In a preferred embodiement,the solvent is present in a fungicidal composition is solvent c-9.
The atleast a solvent weight concentration in said fungicidal composition is in range of 10-15 %. In a preferred embodiement , the solvent is present in an amout of 12%.

In an embodiment, the fungicidal composition is formulated in a form selected from the group consisting of water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), dry flowables (DF), aqueous Suspoemulsions (SE), capsule suspensions (CS) and microgranules (MG).

In a preferred embodiment, the fungicidal composition is in the form of suspoemulsion (SE).

In a more preferred embodiment, the fungicidal composition of the present invention is formulated in the form ofSuspoemulsion comprising a wetting agent, a dispersing agent, a defoaming agent, a rheology modifier, solvent and a biocide .

The present invention also provides a method for controlling disease in agriculturalcrops, particularly in rice crop, said method comprising treating rice crop with afungicidal composition comprising a) Azoxstrobin b) tebuconazole and c)Isoprothiolane , wherein Azoxystrobin is present in an amount ranging from 1-20% %w/w, Tebuconazole is present in an amount ranging from 1-25 % w/w andIsoprothiolane is present in an amount ranging from 1-25 % w/w of the fungicidalcomposition.

In a preferred embodiment, Azoxstrobin is present in an amount of 7.5% w/w,Tebuconazole is present in an amount of 12% w/w and Isoprothiolane is present in an amount of 12% w/w of the fungicidal composition. The fungicidal compositionis as described substantially in the present disclosure.
The present invention further provides a process of preparing a fungicidalcomposition comprising a) Azoxystrobin b) Tebuconazole and c) Isoprothiolane ,wherein Azoxystrobin is present in an amount ranging from 1-20% w/w, Tebuconazole is present in an amount ranging from 1-25% w/w and Isoprothiolane ispresent in an amount ranging from 1-25% w/w of the fungicidal composition; as a Suspoemulsion formulation, said process comprising the following steps:
1) The desired quantity of demineralised water were weighed and polyethyleneglycol, polymericdispersant and polymeric co-dispersant were added and then azoxystrobin and tebuconazole added into it under stirring for 30 min to obtained suspension concentration formulation
2) The C9 solvent was taken in a separate kettle and mixed isoprothiolane to obtain Emulsifiable concentrate. (Ec) formulation.
3) Mixed both EC and SC formulation for 1 hour in a separate kettle to form homogenous suspension
4) After getting homogenous suspension form add freshly prepared carbopol and silicon defoamer and benzisothiazoline.

EXAMPLES
Chemical Composition Percentage w/w
Azoxystrobin technical (Basis of 100.0%) 7.5
Tebuconazole technical
(Basis of 100%) 12
Isoprothiolane technical
(Basis of 100%) 12
Polyethylene glycol 08
Polymeric dispersant 03
Polymeric co dispersant 02
Benzisothiazolinone 0.2
Silicon Based Defoamer 0.2
Carbopol (rheology modifier) 0.2
Sovent C-IX 12
Dimineralised water QS

The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
Example 1: Preparation of fungicidal composition as Suspoemulsion (SE)
Table 1 below provides a fungicidal composition as described in the present specification, formulated as a Suspoemulsion (SE)

Preparation method of SE
The desired quantity of demineralised water were weighed and polyethylene glycol ,polymeric dispersant and polymeric co-dispersant were added and then azoxystrobin and tebuconazole added into it under stirring for 30 min to obtained suspension concentration formulation .The C9 solvent was taken in a separate kettle and mixed isoprothiolane to obtained Emulsifiable concentrate .(Ec) formulation .Mixed both EC and SC formulation for 1 hour in a separate kettle to form homogenous suspension .After getting homogenous suspension form add freshly prepared carbopol, Benzisothiazolinone and silicon defoamer .
Fig 1 shows a flow diagram representation of the steps involved in the preparation of the SC Formulation was tested for quality parameters as per the standard protocols and, the important quality parameters are listed below.

Table 2: Quality parameters of SC formulation
Sno Parameters Parameter Desired quality

1. Description The material shall be in the form of white to free flowing suspension
2.

Active ingredients contents Azoxystrobin -7.5% m/m (±10%)
Tebuconazole -12% m/m (±6%)
Isoprothiolane - 12% m/m (±6%)
3. Persistent Foaming not more than 60 ml after 12 min
4. PH of the 1 % Aqueous solution 4-9 sec.
5. Suspensibility should not be less than 90% w/w.
6. Pourability 05% Max
7. Wet Seive Min. 98% through 75µm sieve.
8. Heat stability test 100ml sample & keep it in 54°C±2 for 14 days.
9 Cold test 100ml sample & keep it in 5°C±2 for 14 days.

Example 2: Field evaluation of the bioefficacy of the present fungicidal composition.
Season Kharif 2022-23
Location of experiment Karnal (Haryana)
Crop Rice
Variety Pusa -1509
Plot size 25 m2
Design RBD
Replication 3
Treatments 9
Date of transplanting 05-07-2022
Application 1st application just after initiation of disease (40-50 DAT of Rice) and followed by 15 day intervals.
.
Application Method: Measured quantity of the chemical was added to required volume of water @ 500L/ha for spraying. The spray tank was filled with ½ the quantity of clean required volume of water and then the measured chemical (according to the dose) was added followed by the rest half quantity of water. The solution was stirred well and thorough coverage were ensured

Example 3:
Table 3a below provides the treatment details of the Rice crop in the trial experiment conducted. For purposes of convenience, the present composition has been represented by the code (JU751212SE.

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha)
T-1 Untreated - -
T-2 Azoxystrobin 23% SC 125 545ml
T-3 Tebuconazole 25.9% EC 187.5 725 ml
T-4 Isoprothiolane 40% EC 300 750 ml
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml
T-6 (JU 751212) SE 45 + 72 + 72 600 ml
T-7 (JU 751212) SE 56.25 + 90 + 90 750 ml
T-8 (JU 751212) SE 67.50 + 108 + 108 900 ml
T-9 (JU 751212) SE 81 + 129.60 + 129.60 1080 ml

Note: (JU751212) SE : Azoxystrobin 7.5% + Tebuconzole12% + Isoprothiolane 12% SE

Table 3b below provides the treatment details of the rice crop in the trial experiment conducted. For purposes of convenience, the present composition has been represented by the “(JU 5599)SE”

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha)
T-1 Untreated - -
T-2 Azoxystrobin 23% SC 125 545 ml
T-3 Tebuconazole 25.9% EC 187.5 725 ml
T-4 Isoprothiolane 40% EC 300 750 ml
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml
T-6 (JU 5599)SE 33 + 54 + 54 600 ml
T-7 (JU 5599)SE 41.25 + 67.5 + 67.5 750 ml
T-8 (JU 5599)SE 49.50 + 81 + 81 900 ml
T-9 (JU 5599)SE 59.40 + 97.20 + 97.20 1080 ml

Note: (JU 5599)SE: Azoxystobin 5.5% + Tebuconazole 9% + Isoprothiolane 9% SE

Table 3c below provides the treatment details of the rice crop in the trial experiment conducted. For purposes of convenience, the present composition has been represented by the Code: (JU 951515 SE)”

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha)
T-1 Untreated - -
T-2 Azoxystrobin 23% SC 125 545 ml
T-3 Tebuconazole 25.9% EC 187.5 725 ml
T-4 Isoprothiolane 40% EC 300 750 ml
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml
T-6 JU 951515 SE 57 + 90 + 90 600 ml
T-7 JU 951515 SE 71.25 + 112.5 + 112.5 750 ml
T-8 JU 951515 SE 85.5 + 135 + 135 900 ml
T-9 JU 951515 SE 102.6 + 162 + 162 1080 ml

Note: Azoxystrobin 9.5% + Tebuconazole 15% + Isoprothiolane 15% SE
EVALUATION OF BIO-EFFICACY AGAINST DISEASE MANAGEMENT

a) Evaluation of Bioefficacy of Sheath blight, Blast and Brown leaf spot diseases of Paddy

Observations will be recorded for disease severity in each treatment before and after each spray as per the standard method. The disease severity will be measured on leaves using 0-9 severity scale of “Standard Evaluation System” (SES 0-9, IRRI, 1996) for Leaf blast, Sheath blight and Brown leaf spot diseases and expressed as Percent Disease Index (PDI) using appropriate formulae.

Scale for Sheath blight disease (0-9)
Sno. Description Score
1 No infection 0
2 Vertical spread of the disease up to 20% of plant height 1
3 Vertical spread of the disease up to 21-30% of plant height 3
4 Vertical spread of the disease up to 31-45% of plant height 5
5 Vertical spread of the disease up to 46-65% of plant height 7
6 Vertical spread of the disease more than 66% of plant height 9

Scale for Leaf blast disease (0-9)
Score Symptoms
0 No visible symptoms
1 Small brown specs of pin point size or larger brown specs without sporulation center
3 Small, roundish to slightly elongated necrotic sporulation spots, about 1-2 mm in diameter with a distinct brown margin or yellow halo.
5 Narrow elliptical lesions, 1-2 mm in breadth, more than 3 mm long with a brown margin
7 Broad spindle shaped lesion with yellow, brown or purple margin
9 Rapidly coalescing small, whitish, grayish or bluish lesions without distinct margins

Scale for Brown leaf spot disease (0-9)
Score Description
0 No incidence
1 Less than 1% leaf area affected
2 1-3% leaf area affected
3 4-5 % leaf area affected
4 6-10% leaf area affected
5 11-15% leaf area affected
6 16-25% leaf area affected
7 26-50 % leaf area affected
8 51-75% leaf area affected
9 76-100% leaf area affected

Sum of all the numerical disease rating
% Disease Index (PDI) = ---------------------------------------------------------------------------------------X 100

Total No. of plants (10) X Maximum disease grade in scale (9)

% Disease Control= Control (PDI) - Treated Plot (PDI)
---------------------------------------------------------------------------- x 100
Control (PDI)

Table-4: Synergistic effect of fungicidal combination of “Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE”( JU 751212 SE ) against Sheath blight disease on PaddyCrop(After First and Second Application).

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control PDI (15DAA2) % Control
T-1 Untreated - - 0.14 0.60 0.00 0.81 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.15 0.30 50.75 0.31 62.22
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.14 0.28 53.73 0.29 64.44
T-4 Isoprothiolane 40% EC 300 750 ml 0.15 0.33 44.78 0.33 58.89
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.14 0.14 76.12 0.14 82.22
T-6 JU 751212 SE 45 + 72 + 72 600 ml 0.15 0.17 71.64 0.19 76.67
T-7 JU 751212 SE 56.25 + 90 + 90 750 ml 0.14 0.14 76.12 0.14 82.22
T-8 JU 751212 SE 67.50 + 108 + 108 900 ml 0.15 0.14 76.12 0.14 82.22
T-9 JU 751212 SE 81 + 129.60 + 129.60 1080 ml 0.14 0.14 76.12 0.14 82.22
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application

Table-5: Synergistic effect of fungicidal combination of “Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE”( JU -751212 ) against Leaf blastdisease on PaddyCrop(After First and Second Application).

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control PDI (15DAA2) % Control
T-1 Untreated - - 0.09 0.51 0.00 0.81 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.10 0.21 59.65 0.30 63.33
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.10 0.22 57.89 0.31 62.22
T-4 Isoprothiolane 40% EC 300 750 ml 0.09 0.14 71.93 0.23 71.11
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.10 0.10 80.70 0.10 87.78
T-6 JU -751212 45 + 72 + 72 600 ml 0.10 0.14 73.68 0.16 80.00
T-7 JU -751212 56.25 + 90 + 90 750 ml 0.10 0.10 80.70 0.10 87.78
T-8 JU -751212 67.50 + 108 + 108 900 ml 0.09 0.10 80.70 0.10 87.78
T-9 JU -751212 81 + 129.60 + 129.60 1080 ml 0.10 0.10 80.70 0.10 87.78
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application

Table-6: Synergistic effect of fungicidal combination of“Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE” (JU 751212SE) against Brown leaf spotdisease on PaddyCrop(After First and Second Application).

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control
PDI (15DAA2) % Control
T-1 Untreated - - 0.10 0.41 0.00 0.77 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.10 0.14 66.67 0.24 68.60
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.10 0.14 64.44 0.25 67.44
T-4 Isoprothiolane 40% EC 300 750 ml 0.09 0.17 57.78 0.28 63.95
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.10 0.11 73.33 0.11 86.05
T-6 JU 751212 SE 45 + 72 + 72 600 ml 0.10 0.13 68.89 0.14 81.40
T-7 JU 751212 SE 56.25 + 90 + 90 750 ml 0.10 0.11 73.33 0.11 86.05
T-8 JU 751212 SE 67.50 + 108 + 108 900 ml 0.09 0.11 73.33 0.11 86.05
T-9 JU 751212 SE 81 + 129.60 + 129.60 1080 ml 0.10 0.11 73.33 0.11 86.05
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %;Disease Index BA: Before Application; DAA: Days After Application

Table 4 to 6 (Protocol-A):
The results shown in Table 4 to 6 highlight the promising efficacy of the recently developed Novel Trimix-Fungicide Combination, labeled as JU 751212SE. This combination contains a blend of three active ingredients: Azoxystrobin at 7.5%, Tebuconazole at 12%, and Isoprothiolane at 12%, in a suspoemulsion (SE)form. The study assessed the effectiveness of this combination against Sheath blight, Leaf blast and Brown leaf spot diseases in Rice crop, comparing it to market-standard products used individually, in combination, or untreated controls.

Notably, the Novel Trimix-Fungicide Combination (T-7 to T-9) exhibited remarkable results when applied at a 15-day interval. This regimen yielded the highest percentage of disease control following the 1st and 2nd applications, surpassing the performance of market-standard products used alone (T-2 to T-4). Additionally, even a lower dosage of the Novel Trimix-Fungicide Combination (T-6) demonstrated superior efficacy compared to individual market-standard products and untreated controls, all without causing any adverse effects on the Rice crop.

This synergistic effect, combined with the specific ratio and composition of the ingredients, likely contributed to the observed efficacy against Sheath blight, Leaf blast and Brown leaf spot. The results suggest that the combination's unique formulation led to enhanced disease control without harming the Rice crop.

Evaluation of Phytotoxicity
Visual observations were recorded at 3, 7 and 10 days after application (DAA). The parameters observed were leaf injury on tip/surface, necrosis, vein clearing, epinasty, hyponasty and wilting. For the rating on phytotoxicity the core Scale of 0–10 was followed for leaf injury on tips/surface.

Phytotoxicity symptoms Scoring and rating for leaf injury on tip/surface
Leaf injury on tips/surface Rating
0% 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10

Table-7: Phytotoxicity of Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE (JU 751212 SE )against Sheath blight disease on Paddy Crop(After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 751212 SE 45 + 72 + 72 600 ml 0 0 0 0 0 0
T-7 JU 751212 SE 56.25 + 90 + 90 750 ml 0 0 0 0 0 0
T-8 JU 751212 SE 67.50 + 108 + 108 900 ml 0 0 0 0 0 0
T-9 JU 751212 SE 81 + 129.60 + 129.60 1080 ml 0 0 0 0 0 0

Table-8: Phytotoxicity of Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE (JU 751212 SE) against Leaf blastdisease on PaddyCrop(After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 751212 SE 45 + 72 + 72 600 ml 0 0 0 0 0 0
T-7 JU 751212 SE 56.25 + 90 + 90 750 ml 0 0 0 0 0 0
T-8 JU 751212 SE 67.50 + 108 + 108 900 ml 0 0 0 0 0 0
T-9 JU 751212 SE 81 + 129.60 + 129.60 1080 ml 0 0 0 0 0 0

Table-9: Phytotoxicity of Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE (JU 751212 SE )against Brown leaf spot disease on Paddy crop(After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 751212 SE 45 + 72 + 72 600 ml 0 0 0 0 0 0
T-7 JU 751212 SE 56.25 + 90 + 90 750 ml 0 0 0 0 0 0
T-8 JU 751212 SE 67.50 + 108 + 108 900 ml 0 0 0 0 0 0
T-9 JU 751212 SE 81 + 129.60 + 129.60 1080 ml 0 0 0 0 0 0

Table 7 to 9:
Phytotoxicity: The success of the Novel Trimix-Fungicide Combination can be attributed to potential synergies among the three distinct active molecules in the formulation. This synergistic effect, combined with the specific ratio and composition of the ingredients, likely contributed to the observed efficacy against Sheath blight, Leaf blast and Brown leaf spotdiseases in Rice crop. The results suggest that the combination's unique led to enhanced disease control without phytotoxcity .
Interestingly, combining the products of the three market-standard treatments (T-5) as a tank mix also demonstrated good disease control effects. However, this approach resulted in significant phytotoxicity, causing leaf injuries, surface tip damage, and necrosis in the Rice plants. This adverse outcome might be attributed to the potential antagonisticinteractions between the various compositions/formulations and ratios of the individual market-standard products, as well as the high concentration of active ingredients in the tank mix.

Table-10: Synergistic effect of fungicidal combination of “Azoxystrobin 5.5% + Tebuconazole 9% + Isoprothiolane 9% SE” (JU 5599SE) against Sheath blight disease on Paddy crop (After First and Second Application).

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control PDI (15DAA2) % Control
T-1 Untreated - - 0.14 0.59 0.00 0.79 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.15 0.30 49.23 0.31 61.36
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.14 0.28 52.31 0.29 63.64
T-4 Isoprothiolane 40% EC 300 750 ml 0.15 0.33 43.08 0.33 57.95
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.14 0.15 73.85 0.15 80.68
T-6 JU5599 SE 33 + 54 + 54 600 ml 0.15 0.19 67.69 0.22 72.73
T-7 JU5599 SE 41.25 + 67.5 + 67.5 750 ml 0.15 0.16 72.31 0.19 76.14
T-8 JU5599 SE 49.50 + 81 + 81 900 ml 0.14 0.16 72.31 0.19 76.14
T-9 JU5599 SE 59.40 + 97.20 + 97.20 1080 ml 0.14 0.16 72.31 0.19 76.14
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application

Table-11: Synergistic effect of fungicidal combination ofAzoxystrobin 5.5% + Tebuconazole 9% + Isoprothiolane 9% SE” (JU5599SE) against Leaf Blast disease on Paddy crop(After First and Second Application).

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control PDI (15DAA2) % Control
T-1 Untreated - - 0.09 0.50 0.00 0.80 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.10 0.21 58.18 0.30 62.92
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.10 0.22 56.36 0.31 61.80
T-4 Isoprothiolane 40% EC 300 750 ml 0.09 0.18 63.64 0.23 70.79
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.10 0.10 80.00 0.10 87.64
T-6 JU 5599 SE 33 + 54 + 54 600 ml 0.10 0.16 67.27 0.18 77.53
T-7 JU 5599 SE 41.25 + 67.5 + 67.5 750 ml 0.10 0.14 70.91 0.16 79.78
T-8 JU 5599 SE 49.50 + 81 + 81 900 ml 0.09 0.14 70.91 0.16 79.78
T-9 JU 5599 SE 59.40 + 97.20 + 97.20 1080 ml 0.10 0.14 70.91 0.16 79.78
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application

Table-12: Synergistic effect of fungicidal combination of “Azoxystrobin 5.5% + Tebuconazole 9% + Isoprothiolane 9% SE” (JU 5599SE) against Brown leaf spot disease on Paddy Crop (After First and Second Application).

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control PDI (15DAA2) % Control
T-1 Untreated - - 0.09 0.39 0.00 0.75 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.09 0.14 65.12 0.24 67.47
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.10 0.14 62.79 0.25 66.27
T-4 Isoprothiolane 40% EC 300 750 ml 0.09 0.16 58.14 0.28 62.65
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.09 0.11 72.09 0.11 85.54
T-6 JU 5599SE 33 + 54 + 54 600 ml 0.10 0.14 65.12 0.19 74.70
T-7 JU 5599SE 41.25 + 67.5 + 67.5 750 ml 0.09 0.13 67.44 0.16 78.31
T-8 JU 5599SE 49.50 + 81 + 81 900 ml 0.10 0.13 67.44 0.16 78.31
T-9 JU 5599SE 59.40 + 97.20 + 97.20 1080 ml 0.10 0.13 67.44 0.16 78.31
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application

Table 10 to 12 :
Results from Table 10 to 12 shown that the Trimix-Fungicidal Combination (coded as JU 5599 SE) demonstrated good control of Rice Sheath blight, Leaf blast and Brown leaf spot diseases in Rice crop after the first and second applications, with a 15-day interval between applications. The combination performed better than the market-standard products applied alone and the untreated control. but this cause phytotoxicity to the crop (Shown in the below table)
Table-13: Phytotoxicity of Azoxystrobin 5.5% + Tebuconazole 9% + Isoprothiolane 9% Code as JU 5599SE against Sheath blight disease on Paddy Crop (After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 5599SE 33 + 54 + 54 600 ml 1 2 5 4 0 7
T-7 JU 5599SE 41.25 + 67.5 + 67.5 750 ml 2 6 6 7 6 0
T-8 JU 5599SE 49.50 + 81 + 81 900 ml 0 4 5 2 0 6
T-9 JU 5599SE 59.40 + 97.20 + 97.20 1080 ml 4 6 4 5 0 5
g a.i./ha: gram of active ingredient per hectare; DAA: Days After Application

Table-14: Phytotoxicity of Azoxystrobin 5.5% + Tebuconazole 9% + Isoprothiolane 9% JU 5599SEagainst Leaf blastdisease on PaddyCrop (After First and Second Application)

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 5599SE 33 + 54 + 54 600 ml 3 2 2 3 7 4
T-7 JU 5599SE 41.25 + 67.5 + 67.5 750 ml 1 3 4 3 5 5
T-8 JU 5599SE 49.50 + 81 + 81 900 ml 1 2 3 5 6 5
T-9 JU 5599SE 59.40 + 97.20 + 97.20 1080 ml 2 3 2 3 4 5
g a.i./ha: gram of active ingredient per hectare; DAA: Days After Application

Table-15: Phytotoxicity of Azoxystrobin 5.5% + Tebuconazole 9% + Isoprothiolane 9% (Code as JU 5599SE)against Brown leaf spot disease on Paddy Crop (After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 5599SE 33 + 54 + 54 600 ml 3 4 0 6 6 0
T-7 JU 5599SE 41.25 + 67.5 + 67.5 750 ml 1 2 3 5 4 6
T-8 JU 5599SE 49.50 + 81 + 81 900 ml 3 6 4 7 4 4
T-9 JU 5599SE 59.40 + 97.20 + 97.20 1080 ml 3 0 4 0 5 3
g a.i./ha: gram of active ingredient per hectare; DAA: Days After Application

Phytotoxic Effects: The Table no 13 to 15 shown that the Trimix-Fungicidal Combination (JU 5599SE) caused phototoxic effects on the Rice crop, indicating that it harm the plants' growth or health.

Table-16: Synergistic effect of fungicidal combination of JU 951515SE“Azoxystrobin 9.5% + Tebuconazole 15% + Isoprothiolane 15% SE”against Sheath blight disease on PaddyCrop(After First and Second Application).
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control

PDI (15DAA2) % Control

T-1 Untreated - - 0.14 0.62 0.00 0.83 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.15 0.30 52.17 0.31 63.04
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.14 0.28 55.07 0.29 65.22
T-4 Isoprothiolane 40% EC 300 750 ml 0.15 0.33 46.38 0.34 58.70
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.14 0.15 75.36 0.15 81.52
T-6 JU 951515SE 57 + 90 + 90 600 ml 0.15 0.16 73.91 0.16 80.43
T-7 JU 951515SE 71.25 + 112.5 + 112.5 750 ml 0.15 0.15 75.36 0.15 81.52
T-8 JU 951515SE 85.5 + 135 + 135 900 ml 0.14 0.15 75.36 0.15 81.52
T-9 JU 951515SE 102.6 + 162 + 162 1080 ml 0.14 0.15 75.36 0.15 81.52
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application

Table-17: Synergistic effect of fungicidal combination of “Azoxystrobin 9.5% + Tebuconazole 15% + Isoprothiolane 15% SE”(JU 951515SE )against Leaf blastdisease on PaddyCrop(After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control

PDI (15DAA2) % Control

T-1 Untreated - - 0.09 0.50 0.00 0.82 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.10 0.21 58.93 0.30 63.74
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.10 0.22 57.14 0.31 62.64
T-4 Isoprothiolane 40% EC 300 750 ml 0.09 0.18 64.29 0.24 70.33
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.10 0.10 80.36 0.10 87.91
T-6 JU 951515SE 57 + 90 + 90 600 ml 0.10 0.11 78.57 0.11 86.81
T-7 JU 951515SE 71.25 + 112.5 + 112.5 750 ml 0.10 0.10 80.36 0.10 87.91
T-8 JU 951515SE 85.5 + 135 + 135 900 ml 0.09 0.10 80.36 0.10 87.91
T-9 JU 951515SE 102.6 + 162 + 162 1080 ml 0.10 0.10 80.36 0.10 87.91
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application
Table-18: Synergistic effect of fungicidal combination of JU 951515SE “Azoxystrobin 9.5% + Tebuconazole 15% + Isoprothiolane 15% SE”against Brown leaf spotdisease on Paddy Crop (After First and Second Application).

Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) PDI (BA1) PDI (15DAA1) % Control PDI (15DAA2) % Control
T-1 Untreated - - 0.09 0.37 0.00 0.73 0.00
T-2 Azoxystrobin 23% SC 125 545 ml 0.09 0.13 65.85 0.23 67.90
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0.10 0.14 63.41 0.24 66.67
T-4 Isoprothiolane 40% EC 300 750 ml 0.09 0.15 58.54 0.27 62.96
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 0.09 0.09 75.61 0.09 87.65
T-6 JU 951515SE
57 + 90 + 90 600 ml 0.10 0.10 73.17 0.10 86.42
T-7 JU 951515SE
71.25 + 112.5 + 112.5 750 ml 0.09 0.09 75.61 0.09 87.65
T-8 JU 951515SE
85.5 + 135 + 135 900 ml 0.10 0.09 75.61 0.09 87.65
T-9 JU 951515SE 102.6 + 162 + 162 1080 ml 0.10 0.09 75.61 0.09 87.65
g a.i./ha: gram of active ingredient per hectare; PDI: Percent Disease Index %; Disease Index BA: Before Application; DAA: Days After Application

Table 16 to 18 (Protocol-C):
Results from Table 16 to 18: The results from these tables show that the Trimix-Fungicidal Combination (coded as JU 951515 SE) also provided good control of Sheath blight, Leaf blast and Brown leaf spot diseases in Rice crop after the first and second applications, with a 15-day interval between applications. The combination performed better than the market-standard products applied alone and the untreated control.

Table-19: Phytotoxicity of Azoxystrobin 9.5% + Tebuconazole 15% + Isoprothiolane 15% JU951515SEagainst Sheath blight disease on Paddy crop(After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 951515SE 57 + 90 + 90 600 ml 1 2 3 3 3 3
T-7 JU 951515SE 71.25 + 112.5 + 112.5 750 ml 1 2 3 3 3 3
T-8 JU 951515SE 85.5 + 135 + 135 900 ml 2 3 3 3 3 3
T-9 JU 951515SE 102.6 + 162 + 162 1080 ml 2 3 4 4 4 4
g a.i./ha: gram of active ingredient per hectare; DAA: Days After Application
Table-20: Phytotoxicity of Azoxystrobin 9.5% + Tebuconazole 15% + Isoprothiolane 15% ( JU 951515SE) against Leaf blast disease on PaddyCrop (After First and Second Application)
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 951515SE 57 + 90 + 90 600 ml 1 2 3 3 3 3
T-7 JU 951515SE 71.25 + 112.5 + 112.5 750 ml 1 2 3 3 3 3
T-8 JU 951515SE 85.5 + 135 + 135 900 ml 2 3 3 3 3 3
T-9 JU 951515SE 102.6 + 162 + 162 1080 ml 2 3 4 4 4 4
g a.i./ha: gram of active ingredient per hectare; DAA: Days After Application

Table-21: Phytotoxicity of Azoxystrobin 9.5% + Tebuconazole 15% + Isoprothiolane 15% SE (JU 951515SE)against Brown leaf spot disease on Paddy crop(After First and Second Application).
Sr. No. Treatment details Dose (g a.i./ha) Dose (Formulation/ha) 3 DAA1 7 DAA1 10 DAA1 3 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Azoxystrobin 23% SC 125 545 ml 0 0 0 0 0 0
T-3 Tebuconazole 25.9% EC 187.5 725 ml 0 0 0 0 0 0
T-4 Isoprothiolane 40% EC 300 750 ml 0 0 0 0 0 0
T-5 Azoxystrobin 23% SC + Tebuconazole 25.9% EC + Isoprothiolane 40% EC 125 + 187.5 + 300 2020 ml 2 3 4 5 6 7
T-6 JU 951515SE 57 + 90 + 90 600 ml 1 2 3 3 3 3
T-7 JU 951515SE 71.25 + 112.5 + 112.5 750 ml 1 2 3 3 3 3
T-8 JU 951515SE 85.5 + 135 + 135 900 ml 2 3 3 3 3 3
T-9 JU 951515SE 102.6 + 162 + 162 1080 ml 2 3 4 4 4 4
g a.i./ha: gram of active ingredient per hectare; DAA: Days After Application
Table 19 to 21
Phytotoxic Effects: The trimix combination (JU 951515SE) performed better than the market-standard products applied alone and the untreated control. However, there were significant phytotoxic effects observed as a result of using the Trimix-Fungicidal Combination. These effects included leaf injuries, surface tip damage, and necrosis in the Rice plants.

Result and Conclusion:

Evaluation of bio-efficacy of Sheath blight, Leaf blast&Brown leaf spot diseases of Rice crop:
Results shown in Table 4 to 6 indicated that Novel Trimix-Fungicide Combination (Coded as JU 751212 SE) that comprises of “Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE” (T-7 to T-9), when applied at 15 days interval, surprisingly provided maximum percent and longer duration control of Sheath blight, Leaf blast and Brown leaf spot diseases of Rice crop after 1st and 2nd applications in comparison to the products of market-standard applied alone (T-2 to T-4) followed by lower dose (T-6) of “Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE” @ 600ml/ha (45 + 72 + 72g a.i./ha)and untreated control (T-1), without causing any phytotoxic effect on Rice crop.
It might be the result of positive synergy and unique ratio/composition of all three different molecules when tested at different dosages.
Simultaneously, when combination of the products of all three market-standard (T-5) applied together as tank-mixed also provided good control of Rice Sheath blight, Leaf blast and Brown leaf spot diseases, but caused high phytotoxic effect in terms of leaf injury on tip/surface and necrosis on Rice crop. It might be the result of antagonistic effect of different composition/formulation of individual product contained in different ratio as well as high concentration of active ingredients.
Overall, Treatment-7 of the disclosed Novel Trimix-Fungicidal Synergistic Combination/Formulation of “Azoxystrobin 7.5% + Tebuconazole 12% + Isoprothiolane 12% SE” @ 750ml/ha (56.25 + 90 + 90g a.i./ha) followed by T-8 and T-9, without causing any phytotoxic effect on Rice crop was observed most suitable, effective and economical for the control of Rice Early and Late blight diseases in comparison to other tested combinations.
,CLAIMS:
1) A fungicidal composition comprising a) Azoxstrobin as first active ingredient b) tebuconazole as second active ingredient and c) Isoprothiolane as third active ingredient, wherein Azoxstrobin is present in an amount ranging from 1-20%w/w, Tebuconazole is present in an amount ranging from 1-25%w/w, and Isoprothiolane is present in an amount ranging from 1-25%w/w.
2) The fungicidal composition as claimed in claim 1, further comprising at least one agriculturally acceptable excipient.
3) The fungicidal composition as claimed in claim 1, wherein said fungicidal composition is formulated in a form selected from the group consisting of water- soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), water-dispersible granules (WG), water- soluble granules (SG), wettable powders (WP), water soluble powders (SP), dry flowables (DF), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG),aqueous Suspoemulsions (SE), capsule suspensions (CS) and microgranules (MG).
4) The fungicidal composition as claimed in claim 3, wherein said fungicidal composition is in the form of Suspoemulsion (SE) formulation.
5) The fungicidal composition as claimed in claim 4, wherein said fungicidal composition further comprises at least a wetting agent, at least a dispersing agent, at least a defoaming agent, at least a rheology modifier, at least a solvent and at least a biocide.
6) The fungicidal composition as claimed in claim 5, wherein said at least a dispersing agent is selected from the group consisting of sodium lignosulphonates, sodium naphthalene sulphonate formaldehyde condensates, aliphatic alcohol ethoxylates, tristyrylphenol ethoxylates and esters, ethylene oxide/propylene oxide block copolymers, polymeric dispersant and combinations thereof.; and wherein said at least a dispersing agent is present in an amount ranging from 2-5% w/w of the fungicidal composition.
7) The fungicidal composition as claimed in claim 5, wherein said at least a wetting agent is selected from the group consisting of blend of alkyl naphthalene sulfonate, sodium salt, sodium laurel sulphate, polymeric co-dispersant and combinations thereof.; and wherein said wetting agent is present in an amount ranging from 1-4% w/w of the fungicidal composition.
8) The fungicidal composition as claimed in claim 5, wherein said at least an defoaming agent is selected from the group consisting of silicone emulsions, silicon based defoamer long- chain alcohols, fatty acids, organic fluorine compounds, and combinations thereof; and wherein said at least a defoaming agent is present in an amount ranging from 0.1-.5% w/w of the fungicidal composition.
9) The fungicidal composition as claimed in claim 5, wherein said atleast a rheology modifier is selected from the group consisting of clay, fumed silica, cellulosics, acrylics, speacility clays, carbopol and combinations thereof, and wherein said at a carrier is present in an amount ranging from 0.1-0.5% w/w of the fungicidal composition.
10) The fungicidal composition as claimed in claim 5, wherein said at least a biocide is selected from the group consisting of sodium benzoate, 1,2- benzisothiazoline-3-one, 2-5 methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4- isothiazolin-3-one, potassium sorbate, para hydroxy benzoates or mixtures thereof and wherein said at a biocide is present in an amount ranging from 0.1-0.5%.
11) The fungicidal composition as claimed in claim 1, wherein Azoxystrobin is present in an amount of 7.5% w/w, tebuconazole is present in an amount of 12% w/w, and Isoprothiolane is present in an amount of 12% w/w of the fungicidal composition.
12) A process for preparing a fungicidal composition as claimed in claim 1 as a suspoemulsion formulation, said process comprising:
(a) The desired quantity of demineralised water were weighed and polyethylene glycol 8%, polymeric dispersant3% and polymeric co-dispersant 2% were added and then azoxystrobin12% and tebuconazole12% added into it under stirring for 30 min to obtained suspension concentration formulation.
(b) The C9 solvent 12% was taken in a separate kettle and mixed isoprothiolane 12% to obtain Emulsifiable concentrate. (Ec) formulation.
(c) Mixed both EC and SC formulation for 1 hour in a separate kettle to form homogenous suspension.
(d) After getting homogenous suspension form add freshly prepared Benzisothiazolinone .2%, carbopol.2% and silicon defoamer .2%