FORM 2
THE PATENTS ACT, 1970
(39 of 1970)
COMPLETE SPECIFICATION
TITLE OF INVENTION: "A synergistic Hepatoprotective formulation."
NAME OF APPLICANT: Zota Health Care Limited.
ADDRESS OF APPLICANT-
Zota House
2/896, Hira Modi Street, Sagrampura,
Surat - 395002 (Gujarat)
India.
PREAMBLE OF INVENTION:
The following specification particularly describes the nature of the invention and the manner in which it is to be performed.

FIELD OF INVENTION
The present invention is based on a synergistic hepatoprotective formulation which comprises of Glutathione, Cysteine and nimuslide. This formulation works against hepatotoxin which damages the liver.
PRIOR ART
In the existing system as given in (WO/2004/087127) wherein The present invention relates to a synergistic formulation comprising trans-tetracos-15-enoic acid (t-TCA) and andrographolide (AP) in the ratio ranging between 1:7 to 7:1 (w/w), useful in hepatoprotection; and a process for the preparation of a synergistic formulation comprising trans-tetracos-15-enoic acid (t-TCA) and andrographolide (AP) useful in hepatoprotection, said process comprising steps of grounding the particles of the t-TCA and AP into fine particles, mixing the fine particles in ratio ranging between 1:7 to 7:1 (w/w), and grinding the mixture to obtain formulation, and also, a method of hepatoprotection of a subject using the synergistic formulation comprising trans-tetracos-15-enoic acid (t-TCA) and andrographolide (AP), said method comprising the steps of administering the formulation to the subject.
In the existing system as given in(WO/2003/024487) wherein There is provided a method of increasing the presence of glutathione in cells including treating or preventing of impaired liver function by administering to a mammal in the need thereof a dietetic or pharmaceutical composition and to a corresponding dietetic or pharmaceutical composition. Said composition contains at least one of each of the following components a) a precursor of glutathione which is selected from the group consisting of cysteine and functional equivalents containing said amino acid; b) an enhancer of the glutathione biosynthesis; and c) a third component which is either selected from lignans or which is a combination cl) of an enhancer of glutathione regeneration and c2) of an enhancer of the glutathione-mediated conjugation.
In the existing system as given in United States Patent 6913772 wherein The present invention relates to a composition useful for hepatoprotection, said composition comprising polar extract of plant Cryptolepis Buchanani and/or fractions of the said extract, and optionally pharmaceutically acceptable additives and a method of producing said composition and also a method of treating a subject using said composition.
In the existing system as given in United States Patent 6989162 wherein A process of isolation of pure Acteoside of high hepatoprotection from plant Colerbrookea oppositifolia, said process comprising steps of drying aerial parts of the plant, grounding the dried parts into powder, percolating the powder with water or ethanol for 3-4 times to obtain an extract, filtering the extract for clearing of suspended particles to obtain supernatant, drying the supernatant at about 45 to 55° C. to obtain a residue, fractionating the residue with chloroform, ethyl acetate, and butanol successively, subjecting butanol fraction to adsorption chromatography of Si02 after adding methanol to the fraction, charging the adsorbed fraction to glass column, eluting the column with solvents of increasing polarity of methanol .chloroform to obtain further fractions and repeating the process one more time, subjecting the fractions to column chromatography to obtain fractions, concentrating the fractions under reduced pressure to obtain acteoside as residue; and a method of effectively hepatoprotecting a subject using pure Acteoside from plant Colerbrookea oppositifolia, said method comprising steps of administering appropriate low-dose

In the existing system as given in United States Patent 7078064 wherein The invention relates generally to compositions comprising antioxidants useful for reducing oxidative stress and lipid peroxidation, and treating chronic liver disease, chronic hepatitis C virus infection and nonalcoholic steatohepatitis. In particular, the invention relates to the preparation and oral administration of compositions comprising glycyrrhizin, schisandra, ascorbic acid, L-glutathione, silymarin, lipoic acid, and d-alpha-tocopherol. The invention also relates to the preparation and parenteral administration of compositions comprising glycyrrhizin, ascorbic acid, L-glutathione, and vitamin B-complex, preferably by infusion or intravenous injection. The invention further relates to methods of using the antioxidants, oral compositions and parenteral compositions.
In the existing system as given in United States Patent 7625587 wherein Phytoceutical composition for the prevention and treatment of hepatic disorders. A specific combination of extracts of plants is taught, as well as the principles for the formulations based on categorizing plants into one of three groups, Energy, Bio-Intelligence and Organization. Such combination has synergistic effects, with minimal side effects
In the existing system as given in United States Patent 7199126 wherein The present invention provides compositions of matter, kits and methods for their use in the treatment of MAP kinase-related conditions and/or HMG-CoA reductase-related conditions. In particular, the invention provides compositions for treating inflammatory and/or cardiovascular conditions in an animal subject by inhibiting p38a MAP kinase and/or HMG-CoA reductase, as well as providing formulations and modes of administering such compositions. The invention further provides methods for the rational design of inhibitors of MAP kinase, HMG-CoA reductase, or both for use in the practice of the present invention.
In the existing system as given in United States Patent 7531567 wherein The invention relates to the use of the combination of n-lipoic acid and effectors of the glutathione metabolism for the treatment of disturbances of the cellular thiol status and accompanying disorders in kidney replacement therapy.
In the existing system as given in Patent application number: 20090281093 wherein The present invention relates to novel non steroidal anti-inflammatory compounds (NSAIDs) that release hydrogen sulfide (H.sub.2S). The present invention also provides methods for treating, preventing and/or reducing inflammation-associated diseases by releasing H.sub.2S in the cardiovascular, connective tissue, pulmonary, gastrointestinal, respiratory, urogenital, nervous, or cutaneous systems as well as infective diseases employing said compounds.
In the existing system as given in United States Patent 4868114 wherein A method comprising stimulating the biosynthesis of glutathione in mammalian cells by contacting the cells with an effective amount of a compound of the formula: ##STR1## wherein R is a (CHOH)n CH2 OH and wherein n is 1-5, preferably R is derived from a D-aldose monosaccharide.
THE OBJECT OF THE INVENTION-
The object of the present invention is to introduce the use of an efficient compound mixture which can be used for protection of the liver. This combination has no side effects.

STATEMENT OF INVENTION
Hepatoprotective combination for the protection of liver. This combination comprises of Glutathione, Cysteine and nimuslide. This combination works against hepatotoxin which damages the liver.
DETAILED DESCRIPTION OF THE INVENTION-
The present invention discloses synergistic combination of hepatoprotective ingredients. Which are Glutathione, Cysteine and Nimuslide. The combination is effective against the hepatotoxins which is any toxic chemical substance which damages the liver. Hepatotoxic substances are generally compounds like Pyrrolizidine alkaloids, Luteoskyrin,Aflatoxin,Carbon tetrachloride thereof.
Glutathione, Cysteine and nimuslide are Hepatoprotective compounds. These compounds have Hepatoprotection activity. Hepatoprotection activity of the medicine ascertains that the liver is protected from any potential damage and its smooth functioning is ensured .Hepatoprotective agents are those compounds, which mitigate the liver injury caused by hepatotoxic agents. They help in protection of liver which is a very important digestive organ.
Liver detoxification is very important in order for liver to function well. The liver is the body's major detoxifier. The liver filters all toxins in the body, and eventually excretes it out of our body. If our liver is not functioning well, the toxins that are not excreted out of our body remains in our body. And when toxins freely circulate our body, our body gets sick.
The major functions of the liver:
• Detoxification of chemicals such as drugs, alcohol, and metals
• production of bile that aids in the digestion of fats
• production of blood proteins and other cofactors needed in the production of red blood cells
• storage of glycogen which is used to produce energy
• storage of vitamins A, D and B12
• neutralization of free radicals by endogenous antioxidants
• regulation of hormones
Our liver detoxifies our body 24 hours a day. Glutathione and other endogenous antioxidants work continuosuly in quenching free radicals in the liver. The more free radicals, the more glutathione is used and depleted.
Nimesulide- This compound belongs to a wide class of medicines called anti-inflammatory non steroidal drugs (NSAID). NSAIDs are well known for their anti-pyretic, anti-inflammatory, and analgesic properties. Nimesulide is used to effectively and rapidly provide the relief of inflammation and pain.
Cysteine - It work as a preventative or antidote for some of the negative effects of alcohol, including liver damage and hangover. N-acetyl-L-cysteine (NAC) is a derivative of cysteine wherein an acetyl group is attached to the nitrogen atom. This compound is sold as a dietary

supplement commonly claiming antioxidant and liver protecting effects. Cysteine also helps synthesize glutathione, one of the body's most important natural antioxidants and detoxifiers.
N-Acetyl Cysteine also protects the body from acetaminophen toxicity .It has also been shown to be effective at treating liver failure from other causes as well. N-Acetyl Cysteine is rapidly metabolized to intracellular glutathione.
Glutathione- it is produced in the human liver and plays a key role in intermediary metabolism. Glutathione supplement to effectively raise glutathione levels, it must have ingredients that take care of the stomach, intestines and the liver. The liver is the biggest source of glutathione in the body where it is manufactured and stored. This is why it is very important to support the liver function when taking a glutathione supplement. Glutamirxe helps preserve liver glutathione after a hepatic injury. And it also nourishes the GI tract, liver. Glutathione acts as a powerful antioxidant in the body. Glutathione also detoxifies chemicals into less harmful compounds

Cysteine produce N-Acetyl Cysteine
N-Acetyl Cysteine increases Glutathione Levels
Glutathione detoxifies chemicals into less harmful compounds.

CLAIMS
We claimed
1. "A synergistic hepatoprotective formulation."
2. A synergistic hepatoprotective formulation comprises of Nimesulide, Cysteine and Glutathione.
3. A synergistic hepatoprotective formulation in claimed 2, wherein Nimesulide to effectively and rapidly provide the relief of inflammation and pain.
4. A synergistic hepatoprotective formulation in claimed 2 ,wherein Cysteine work as a preventative or antidote for some of the negative effects of alcohol, including liver damage and hangover and it also helps synthesize glutathione, one of the body's most important natural antioxidants and detoxifiers.
5. A synergistic hepatoprotective formulation in claimed 2 , wherein Glutathione supplement to effectively raise glutathione levels, it must have ingredients that take care of the stomach, intestines and the liver.
6. A synergistic hepatoprotective formulation in claimed 5, wherein it helps preserves liver glutathione after a hepatic injury. And it also nourishes the GI tract, liver. Glutathione acts as a powerful antioxidant in the body. Glutathione also detoxifies chemicals into less harmful compounds.