Claims:1. A synergistic herbal composition for use in treating bloat in ruminant, said composition comprising:
a pharmaceutically effective amount of Trianthema portulacastrum;
a pharmaceutically effective amount of Dolichos lablab;
a pharmaceutically effective amount of Dalbergia sissoo; and
at least one pharmaceutically acceptable excipient.
2. The composition as claimed in claim 1, wherein said composition comprises Trianthema portulacastrum in an amount ranging from about 65% to about 75% by weight of the composition; Dolichos lablab in an amount ranging from about 10% to about 20% by weight of the composition; and Dalbergia sissoo in an amount ranging from about 10% to about 20% by weight of the composition, and wherein any or a combination of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo are in a form selected independently from a group consisting of a powder and an extract.
3. The composition as claimed in claim 1, wherein said composition is formulated in a form selected from a group consisting of a solid, a liquid and a semi-solid.
4. The composition as claimed in claim 1,wherein said composition is formulated in a form selected from a group consisting of a powder, a tablet, a capsule, a syrup, a solution, a suspension, a gel and/or a paste.
5. The composition as claimed in claim 1, wherein said excipient is any or a combination of a bulking agent, a solubilizer, a binder, a disintegrant, a chelating agent, a glidant, a flavouring agent, a colouring agent, a tonicity agent, a sweetening agent, a buffering agent, a preservative, and a co-solvent in an amount ranging from about 1% to about 10% by weight of the composition.
6. A method of preparation of a synergistic herbal composition for use in treating bloat in ruminant, said method comprising the steps of:
(i) taking a pharmaceutically effective amount of each of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo and
(ii) mixing the mixture obtained from step (i) with at least one pharmaceutically acceptable excipient to prepare a herbal composition for use in treating bloat in ruminant.
7. The method as claimed in claim 6, wherein said method comprises Trianthema portulacastrum in an amount ranging from about 65% to about 75% by weight of the composition; Dolichos lablab in an amount ranging from about 10% to about 20% by weight of the composition; and Dalbergia sissoo in an amount ranging from about 10% to about 20% by weight of the composition, and wherein any or a combination of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo are in a form selected independently from a group consisting of a powder and an extract.
8. The method as claimed in claim 6, wherein said composition is formulated in a form selected from a group consisting of a solid, a liquid and a semi-solid.
9. The method as claimed in claim 6, wherein said excipient is any or a combination of a bulking agent, a solubilizer, a binder, a disintegrant, a chelating agent, a glidant, a flavouring agent, a colouring agent, a tonicity agent, a sweetening agent, a buffering agent, a preservative, and a co-solvent in an amount ranging from about 1% to about 10% by weight of the composition.

, Description:
FIELD OF THE INVENTION
[0001] The present invention relates generally to a herbal composition. More specifically, the present invention relates to a synergistic herbal composition for use in treating bloat in ruminants. The present disclosure also provides method(s) for preparation thereof.

BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] Bloat is a digestive disorder characterized by an accumulation of gas in compartment of a ruminant's stomach i.e. rumen. Production of gas, primarily carbon dioxide and methane is a normal result of fermentation processes and the gas is usually discharged by belching. However, if the animal is unable to remove the excess gas, pressure builds up in the rumen-reticulum, exerting pressure on the diaphragm that prevents the animal from inhaling, and bloat occurs. The most common type of bloat is frothy bloat where gas builds up in a foam or froth above the rumen contents and the normal belching is inhibited. Bloat can occur on any forage that is low in fiber and high in protein, but is most common in immature legume pastures. It seldom occurs on grasses, (or pastures with at least 50% grass), coarser pastures, or hay. Bloat usually follows a heavy feeding or grazing period.
[0004] One of the most commonly reported causes of bloat is simply overeating. In this type of bloat, known as dry bloat, gas forms in pockets and is trapped in the upper portions of the rumen and the animal is unable to belch. Another common cause of bloat, especially for ruminants left out on lush pastures are weeds. Milkweeds are just one of several weeds that can produce bloat. Green feed that has grown warm in the stack can also create problems. Foam forms in the rumen with tiny bubbles that are impossible for a ruminant to belch up. Foam is more dangerous than dry bloat.
[0005] Changing the diet of ruminants quickly can also lead to bloat. If an animal is fed with the same basic diet day-in and day-out, their rumen stays adjusted within a certain range of effective digestion. Disrupting this balance can lead to bloat. In young goat kids, bloat can occur when milk replacers are administered as opposed to real goat milk. This especially holds true for milk replacers that are soy based. In any type of bloat, the left side of the ruminant becomes distended and bulges. The ruminants left side will be wider than the right. The symptoms that could be seen in an animal having bloat include signs of pain, such as grinding teeth, salivation, shortness of breath, grunting, depression, or striking out with their legs. Effective digestion process in rumen involves its microflora and desired ruminal environment predominantly controlled by pH. Affected animals commonly show respiratory distress, reduced ruminal motility, distended abdomen measured through abdominal girth. The vitality of rumen microflora and optimum pH has to be taken cared. Medication has to overcome these problems of animals.
[0006] Stomach tubing the animal, thrusting mineral oil, cooking oil or powdered Tide laundry detergent mixed with water down the throat and a few available Oral bloat medications are the most commonly given medications for treating bloat in animals. However these conventional therapeutic agents have their own certain limitations, side effects and toxicity. Therefore, attention is being paid to find alternative approaches. This has prompted interest in finding new, effective, safe herbal compositions including plant to treat bloat in ruminants.
[0007] Trianthema portulacastrum, commonly known as desert horsepurslane, black pigweed, or giant pigweed, is a species in the genus Trianthema belonging to family Aizoaceae. It grows in several continents, including Africa, North and South America, and also present as an introduced species in many other areas. Trianthema portulacastrum was reported to have therapeutic activity against constipation, arthritis, stomach-ache, anemia etc. It is also known to possess anti-inflammatory property.
[0008] Dolichos lablab, commonly known as hyacinth-bean, lablab-bean, is a species in Dolichos genus belonging to the family Fabaceae. It is originated in Asia, but it is now grown for food throughout much of the world. Dolichos lablab was reported to have activity against cholera, vomiting, diarrhoea, leucorrhoea, gonorrhoea, alcoholic intoxication etc.
[0009] Dalbergia sissoo, commonly known as North Indian Rosewood, is a species in genus Dalbergia belonging to family Fabacea. It is native to Indian Subcontinent and Southern Iran. It grows in a wide variety of habitat types, and it can easily take hold in disturbed areas and cultivated land. Dalbergia sissoo was reported in the treatment of gonorrhea and skin ailments.
[0010] There is therefore a need in the art to develop a synergistic herbal composition which is safe and effective against bloat in ruminants, yet devoid of side effects and toxicity so as to rejuvenate the general health and immunity of animals.
[0011] The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art.
[0012] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference.
[0013] The above-described deficiencies of existing medicaments are merely intended to provide an overview of some of the problems of existing medicines, and are not intended to be exhaustive. Other problems with existing medications and corresponding benefits of the various non-limiting embodiments described herein may become further apparent upon review of the following description.

OBJECTS OF THE INVENTION
[0014] An object of the present disclosure is to overcome the disadvantages associated with the conventional composition for treatment of bloating in ruminants.
[0015] Another object of the present disclosure is to provide a synergistic herbal composition that exhibits synergistic activity against bloating in ruminants.
[0016] Another object of the present disclosure is to provide a synergistic herbal composition that is safe.
[0017] Another object of the present disclosure is to provide a synergistic herbal composition that is cost-effective.

SUMMARY
[0018] The following presents a simplified summary of the invention in order to provide a basic understanding of some aspects of the invention. This summary is not an extensive overview of the present invention. It is not intended to identify the key/critical elements of the invention or to delineate the scope of the invention. Its sole purpose is to present some concept of the invention in a simplified form as a prelude to a more detailed description of the invention presented later.
[0019] The present invention relates generally to a herbal composition. More specifically, the present invention relates to a synergistic herbal composition for use in treating bloat in ruminants. The present disclosure also provides method(s) for preparation thereof.
[0020] An aspect of the present disclosure relates to a synergistic herbal composition for use in treating bloat in ruminant, the composition including: a pharmaceutically effective amount of Trianthema portulacastrum; a pharmaceutically effective amount of Dolichos lablab; a pharmaceutically effective amount of Dalbergia sissoo; and at least one pharmaceutically acceptable excipient.
[0021] In an embodiment, the composition includes Trianthema portulacastrum in an amount ranging from about 65% to about 75% by weight of the composition; Dolichos lablab in an amount ranging from about 10% to about 20% by weight of the composition; and Dalbergia sissoo in an amount ranging from about 10% to about 20% by weight of the composition, and wherein any or a combination of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo are in a form selected independently from a group consisting of a powder and an extract.
[0022] In an embodiment, the composition is formulated in a form selected from a group consisting of a solid, a liquid and a semi-solid.
[0023] In an embodiment, the composition is formulated in a form selected from a group consisting of a powder, a tablet, a capsule, a syrup, a solution, a suspension, a gel and/or a paste.
[0024] In an embodiment, the excipient is any or a combination of a bulking agent, a solubilizer, a binder, a disintegrant, a chelating agent, a glidant, a flavouring agent, a colouring agent, a tonicity agent, a sweetening agent, a buffering agent, a preservative, and a co-solvent in an amount ranging from about 1% to about 10% by weight of the composition.
[0025] Another aspect of the present disclosure relates to a method of preparation of a synergistic herbal composition for use in treating bloat in ruminant, the method including the steps of: (i) taking a pharmaceutically effective amount of each of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo and (ii) mixing the mixture obtained from step (i) with at least one pharmaceutically acceptable excipient to prepare a herbal composition for use in treating bloat in ruminant.
[0026] In an embodiment, the method includes Trianthema portulacastrum in an amount ranging from about 65% to about 75% by weight of the composition; Dolichos lablab in an amount ranging from about 10% to about 20% by weight of the composition; and Dalbergia sissoo in an amount ranging from about 10% to about 20% by weight of the composition, and wherein any or a combination of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo are in a form selected independently from a group consisting of a powder and an extract.
[0027] In an embodiment, the composition is formulated in a form selected from a group consisting of a solid, a liquid and a semi-solid.
[0028] In an embodiment, the excipient is any or a combination of a bulking agent, a solubilizer, a binder, a disintegrant, a chelating agent, a glidant, a flavouring agent, a colouring agent, a tonicity agent, a sweetening agent, a buffering agent, a preservative, and a co-solvent in an amount ranging from about 1% to about 10% by weight of the composition.
[0029] Other aspects, advantages, and salient features of the invention will become apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the exemplary embodiments of the invention.

DETAILED DESCRIPTION
[0030] The following description is provided to assist in a comprehensive understanding of exemplary embodiments of the invention. It includes various specific details to assist in that understanding but these are to be regarded as merely exemplary.
[0031] Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope of the invention. In addition, descriptions of well-known functions and constructions are omitted for clarity and conciseness.
[0032] The terms and words used in the following description and claims are not limited to the bibliographical meanings, but, are merely used by the inventor to enable a clear and consistent understanding of the invention.
[0033] Accordingly, it should be apparent to those skilled in the art that the following description of exemplary embodiments of the present invention are provided for illustration purpose only and not for the purpose of limiting the invention as defined by the appended claims and their equivalents.
[0034] It is to be understood that the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise.
[0035] Features that are described and/or illustrated with respect to one embodiment, aspects or implementations may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments aspects or implementations.
[0036] It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, integers, steps or components but does not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.
[0037] The term "synergistic herbal composition" as used herein, intends to cover synergistic herbal composition useful in treating bloat.
[0038] The term "bloat" and the likes include disease and conditions related to an abdomen synergistic herbal composition.
[0039] As used herein, "treatment" and the like generally mean obtaining a desired pharmacological and physiological effect. The effect may be prophylactic in terms of preventing or partially preventing a disease, symptom or condition thereof and/or may be therapeutic in terms of a partial or complete cure of a disease, condition, symptom or adverse effect attributed to the disease. The term "treatment" and the likes as used herein covers any treatment of a disease and includes: (a) preventing the disease from occurring in a subject which may be predisposed to the disease but has not yet been diagnosed as having it; (b) inhibiting the disease, i.e., arresting its development; or relieving the disease, i.e., causing regression of the disease and/or its symptoms or conditions.
[0040] The present invention relates generally to a herbal composition. More specifically, the present invention relates to a synergistic herbal composition for use in treating bloat in ruminants. The present disclosure also provides method(s) for preparation thereof.
[0041] An aspect of the present disclosure relates to a synergistic herbal composition for use in treating bloat in ruminant, the composition including: a pharmaceutically effective amount of Trianthema portulacastrum; a pharmaceutically effective amount of Dolichos lablab; a pharmaceutically effective amount of Dalbergia sissoo; and at least one pharmaceutically acceptable excipient.
[0042] In an embodiment, the composition includes Trianthema portulacastrum in an amount ranging from about 65% to about 75% by weight of the composition; Dolichos lablab in an amount ranging from about 10% to about 20% by weight of the composition; and Dalbergia sissoo in an amount ranging from about 10% to about 20% by weight of the composition, and wherein any or a combination of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo are in a form selected independently from a group consisting of a powder and an extract.
[0043] In an embodiment, the composition is formulated in a form selected from a group consisting of a solid, a liquid and a semi-solid.
[0044] In an embodiment, the composition is formulated in a form selected from a group consisting of a powder, a tablet, a capsule, a syrup, a solution, a suspension, a gel and/or a paste.
[0045] In an embodiment, the excipient is any or a combination of a bulking agent, a solubilizer, a binder, a disintegrant, a chelating agent, a glidant, a flavouring agent, a colouring agent, a tonicity agent, a sweetening agent, a buffering agent, a preservative, and a co-solvent in an amount ranging from about 1% to about 10% by weight of the composition.
[0046] In an embodiment, bulking agent (s) include but not limited to, lactose USP, Starch 1500, mannitol, sorbitol, maltodextrin, malitol or other non-reducing sugars; microcrystalline cellulose (e.g., Avicel), dibasic calcium phosphate (anhydrous or dihydrate), sucrose, etc. and mixtures thereof. However, a person skilled in the art would appreciate that any other bulking agent(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0047] In an embodiment, binder(s) include but not limited to, cellulosic derivatives (such as methylcellulose, carboxymethyl cellulose, hydroxyethyl cellulose, hydroxyethylmethyl cellulose, etc), polyacrylates (such as Carbopol, polycarbophil, etc), Povidone (all grades), Polyox of any molecular weight or grade, irradiated or not, starch, polyvinylpyrrolidone (PVP), Avicel, and the like. However, a person skilled in the art would appreciate that any other binder(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0048] In an embodiment, solubilizer(s) includes but not limited to, cyclodextrins, pH adjusters, salts and buffers, surfactants, fatty acids, phospholipids, metals of fatty acids and the likes. However, a person skilled in the art would appreciate that any other solubilizing agent(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0049] In an embodiment, sweetener(s) includes but not limited to, sucralose, acesulfame-K, aspartame, saccharine or saccharine sodium and calcium salts, sodium cyclamate, sucrose, fructose, glucose, sorbitol, or the mixtures thereof. However, a person skilled in the art would appreciate that any other sweetener(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0050] In an embodiment, flavoring agent (s) includes but not limited to, fruit aromas such as orange, banana, strawberry, cherry, wild cherry, lemon; cardamom, anis, mint, menthol, vanillin, and ethyl vanillin, and other similar aromas, or the mixtures thereof. However, a person skilled in the art would appreciate that any other flavoring agent(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0051] In an embodiment, buffer system includes but not limited to, sodium citrate, potassium citrate, sodium citrate di-hydrate, citric acid, citric acid monohydrate, sodium bicarbonate, potassium bicarbonate, sodium di-hydrogen phosphate and potassium di-hydrogen phosphate and the likes or combination thereof. However, a person skilled in the art would appreciate that any other buffer system can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0052] In an embodiment, glidant(s) includes but not limited to, colloidal silicon dioxide, precipitated silicon dioxide, fumed silica (CAB-O-SIL M-5P, trademark of Cabot Corporation), stearowet and sterotex, silicas (such as SILOID and SILOX silicas—trademarks of Grace Davison Products, Aerosil—trademark of Degussa Pharma), higher fatty acids, the metal salts thereof, hydrogenated vegetable oils and the like. However, a person skilled in the art would appreciate that any other glidant(s) can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0053] In an embodiment, solvents includes but not limited to, methanol, ethanol, n-propanol, isopropanol, hexane, heptane, petroleum ether, cyclohexane, diethyl ether, dusopropyl ether, ethyl acetate, methyl acetate, ethyl formate, methyl formate, isobutyl acetate, n-butyl acetate, methylene chloride, ethylene chloride, chloroform, carbon tetrachloride, acetone, ethyl methyl ketone, diisobutyl ketone, methyl isobutyl ketone, 1,4- dioxane, toluene, ammonia solution, glacial acetic acid, ammonium hydroxide, sodium hydroxide, calcium hydroxide, calcium carbonate, potassium hydroxide, potassium carbonate, water and the likes. However, a person skilled in the art would appreciate that any other solvent or a combination of solvents can be utilized to serve the intended purpose without departing from the scope and spirit of the invention.
[0054] Another aspect of the present disclosure relates to a method of preparation of a synergistic herbal composition for use in treating bloat in ruminant, the method including the steps of: (i) taking a pharmaceutically effective amount of each of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo and (ii) mixing the mixture obtained from step (i) with at least one pharmaceutically acceptable excipient to prepare a herbal composition for use in treating bloat in ruminant.
[0055] In an embodiment, the method includes Trianthema portulacastrum in an amount ranging from about 65% to about 75% by weight of the composition; Dolichos lablab in an amount ranging from about 10% to about 20% by weight of the composition; and Dalbergia sissoo in an amount ranging from about 10% to about 20% by weight of the composition, and wherein any or a combination of Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo are in a form selected independently from a group consisting of a powder and an extract.
[0056] In an embodiment, the composition is formulated in a form selected from a group consisting of a solid, a liquid and a semi-solid.
[0057] In an embodiment, the excipient is any or a combination of a bulking agent, a solubilizer, a binder, a disintegrant, a chelating agent, a glidant, a flavouring agent, a colouring agent, a tonicity agent, a sweetening agent, a buffering agent, a preservative, and a co-solvent in an amount ranging from about 1% to about 10% by weight of the composition

Example
[0058] The present disclosure is further explained in the form of an example. However, it is to be understood that the example is merely illustrative and is not to be taken as limitations upon the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the scope of the invention.
[0059] In an exemplary embodiment of the invention, a 100 g powder mixture was prepared by mixing 68 g powder of Trianthema portulacastrum, 14 g powder of Dolichos lablab and 14 g powder of Dalbergia sissoo. Thereafter, the powder mixture was reconstituted with pharmaceutically acceptable excipients such as 2 g of sweetener and 2 g of binder to obtain the final synergistic herbal composition by methods known per se. The animals were treated and/or fed with the above synergetic herbal composition twice a day for effective synergistic results in bloat treatment.
[0060] In yet another exemplary embodiment of the invention, 25.00g of Trianthema portulacastrum; 5.35g of Dolichos lablab; and 5.35g of Dalbergia sissoo were comprised in a single dose of the herbal composition of the present invention and were administered twice a day to the suffering animals.
[0061] Evaluation of synergistic herbal composition for its ability to treat bloating
[0062] The claimed synergistic herbal composition was evaluated for its ability to reduce symptoms of bloating in naturally bloated goat animal model. The evaluation parameter includes respiration rate (per minute), abdominal girth (in cm), and rumen motility (in cycles per minute). It was also observed that the individual ingredients were not as effective in treating bloat as the composition of the present invention thereby establishing the synergistic effect.
[0063] Treatment Protocol: The animals (goats) were treated with Trianthema portulacastrum, Dolichos lablab, Dalbergia sissoo, and the synergistic herbal composition and are evaluated by different evaluation parameters.
[0064] Observation and Results:

Table 1. Mean ± S. E. of respiration rate per min.
Hour
Treatment 0 Hour 2.5 Hour 8 Hour
Synergistic Herbal Composition 48.16 ± 1.92 44.33 ± 2.33 42.66 ± 1.17

[0065] In the treatment group, the average respiration rate was highest at the 0th hour of the treatment i.e. 48.16 ± 1.92 which was reduced down to 42.66 ± 1.17 at 8th hour post treatment. This indicates that the synergistic herbal composition decreases enhanced respiration rate due to bloat towards the normalcy. However, this reduction in respiration rate was not significant.

Table 2. Mean ± S. E. of abdominal girth in cm
Hour
Treatment 0 Hour 2.5 Hour 8 Hour
Synergistic Herbal Composition 95.50 ± 2.09 95.00 ± 2.22 93.16 ± 2.32

[0066] The average abdominal girth of the bloated goats at 0th hour was 95.50 ± 2.09 which was reduced to 95.00 ± 2.22 at 2.5 hours, and further declined to 93.16± 2.32 at 8 hours. This reduction in abdominal girth was non-significant. At 0th hour increased abdominal girth was recorded which reduced towards normalcy after treatment with synergistic herbal composition. This indicated that the treatment was effective against bloat in goats.

Table 3. Mean ± S. E. of rumen motility in cycles per minute
Hour
Treatment 0 Hour 2.5 Hour
Synergistic Herbal Composition 1.83 ± 0.16 2.00 ± 0.00

[0067] Average rumen motility at 0th hour was 1.83 ± 0.16. This was improved to the 2.00 ± 0.00 at 2.5th hour post treatment with synergistic herbal composition.
[0068] Treatment of naturally bloated goats with synergistic herbal composition reduces respiration rate towards normalcy; but, not significantly. The bloat problem in goats was resolved by reduction in abdominal girth of bloated goats at 8th hour post treatment with synergistic herbal composition. This indicates that synergistic herbal composition is effective in bloat treatment of goats at recommended dose. However, individual herbs (Trianthema portulacastrum, Dolichos lablab, and Dalbergia sissoo) did not show much impact against above mentioned evaluating parameters during 2.5 hour period observation.
[0069] We may conclude that the said synergistic herbal composition effectively treat the bloat symptoms in ruminants.
[0070] Accordingly, the invention is intended to embrace all such alternatives, modifications and variations as may fall within the spirit and scope of the present invention. The foregoing descriptions of specific embodiments of the present invention have been presented for purposes of illustration and description. They are not intended to be exhaustive or to limit the invention to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical application, to thereby enable others skilled in the art to best utilize the invention and various embodiments with various modifications as are suited to the particular use contemplated. It is understood that various omissions, substitutions of equivalents are contemplated as circumstance may suggest or render expedient, but is intended to cover the application or implementation without departing from the spirit or scope of the claims of the present invention.

ADVANTAGES
[0071] The present disclosure provides a synergistic herbal composition to overcome the disadvantages associated with the conventional composition for treatment of bloating in ruminants.
[0072] The present disclosure provides a synergistic herbal composition that exhibits synergistic activity against bloating in ruminants.
[0073] The present disclosure provides a synergistic herbal composition that is safe.
[0074] The present disclosure provides a synergistic herbal composition that is cost-effective.