DESC:FIELD OF THE INVENTION
[001] The invention relates to insecticidal composition, more particularly to a broad spectrum synergistic insecticidal formulation comprising isooxazoline compound and organochlorine compound and a preparation method thereof.

BACKGROUND
Agriculture is an area where many factors influence quantity and quality of produce. One big factor that greatly influences the produce is the infestation of the crops by unwanted pests or insects or fungi. Yields of plants is adversely affected by insect’s attack.

The agriculture sector uses fertilizers, biocides, and genetically modified crops to prevent/control the threat of pests, insects, and fungi and consequently achieve higher yields. The use of biocides is crucial to modern agricultural practices. Plants frequently receive the biocides\pesticides as liquid or solid formulations. Since it has been demonstrated that such control can raise agricultural yields, this approach has experienced significant economic success. The use of biocides has dramatically increased over the last ten years.
However, due to the long-term use of pesticides, many harmful organisms have developed resistance to commonly used pesticides. The crops have become more susceptible to these illnesses/diseases/infestation because of the overuse of this strategy, as the new crops have lost/reduced their natural resilience and the pests and insects have developed tolerance to the biocides.
The agricultural output has significantly decreased because of this resistance. Globally, this kind of resistance is growing, which always lowers crop productivity. Pest/insect species evolve pesticide resistance via natural selection: the most resistant specimens survive and pass on their genetic traits to their offspring.
In addition, the environmental and economic requirements imposed on today's insecticides are constantly increasing with regard to, for example, the range of action, toxicity, selectivity, application rate, formation of residues, and favourable manufacturing capabilities. Due to possible problems (e.g., resistance to known active compounds), an unchanging task is to develop new insecticides that are superior to their known counterparts in at least some aspects.
Because of the growing pests' and insects' resistance to them, the risks to the environment, and worries about worker exposure, successful use of biocides necessitates appropriate management. To successfully control the growth of multiple pests or insects at once, the efficacy of biocides must be increased by extending their spectrum.
Rarely can formulations with a single active ingredient satisfy such broad-spectrum demands. As a result, two or more active compositions are needed. Moreover, such compositions comprising two or more actives have several additional advantages such as reduction in inventory products; saving in time and money; and a reduction in the number of times the crop is sprayed with the insecticide/pesticide. Often this problem, of multiple spraying of a crop, goes unnoticed and results in inconsistent application of both the different actives, thereby obtaining variable results and yields.
Combinations containing different insecticide compositions have been practiced in the art, but problems with the stability of such combinations have caused issues with respect to the application and efficacy in many cases. No single molecule is known to control the target mites, hoppers, thrips, and lepidopteran pests in agricultural and horticultural crops. In addition, a part of known pesticide has high toxicity, or some of them start to disturb native ecosystems due to long-term persistency.
Thus, there is need to develop and improve insecticidal composition for a variety of reasons including: 1) to control a wide spectrum of harmful organisms; 2) to provide a composition having high synergistic action 3) to exhibit low persistency in environment 4) to avoid high loading of the toxicant to the environment and 5) to provide the composition with chemical stability.
Inventors of the present invention have surprisingly found that the synergistic compositions of insecticide selected from class of isooxazoline compound and class of organochlorine compound as described herein in can provide solution to the above problems.

SUMMARY OF THE INVENTION
In one aspect, the present disclosure relates to an insecticidal composition comprising :
a) at least one insecticide selected from class of isooxazoline compound and; b) at least one insecticide selected from class of organochlorine compound with one or more agrochemically acceptable excipients.

In another aspect, the present disclosure relates to a process for preparation of insecticidal composition comprising: a) at least one insecticide selected from class of isooxazoline compound and; b) at least one insecticide selected from class of organochlorine compound with one or more agrochemically acceptable excipients.

DETAILED DESCRIPTION OF THE INVENTION
As used in the present invention, the following terms are generally intended to have the meaning as set forth below, except to the extent that the context in which they are used indicate
It is to be noted that the term "comprising", used in the description and claims are inclusive or open-ended and do not exclude additional, non-recited members, elements or method steps and should not be interpreted as being restricted to the means listed thereafter. It is thus to be interpreted as specifying the presence of the stated features, steps or components as referred to, but does not preclude the presence or addition of one or more other features, steps or components, or groups thereof.
Throughout this specification, reference to "embodiment” indicates that a particular feature or function respectively, described in relation to the embodiment or aspect is included in at least one embodiment of the present invention. Thus, appearances of the phrases "in one embodiment" or "in preferred embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment.
Wherever ranges are provided in the description or claims of the invention referring to measuring component it will encompass all of variations preferably more or less of and from the specified value, insofar such variations are appropriate to perform in the disclosed invention. These variations can be decided by a person ordinarily skilled in art.
Dependent claims features may be combined with features of the other independent or dependent claims or with the features mentioned in the description as appropriate for interpretation of scope and enablement of the claims and description. Furthermore, the features or characteristics may be combined in any suitable manner in one or more embodiments, as would be apparent to one of ordinary skill in the art.
otherwise.
The present invention provides a synergistic insecticidal composition which exhibits greatly enhanced insecticidal activity as compared to the activity of the individual components. The composition also exhibits excellent inhibitory activity against pests/insects, improve crop health and yield, efficiently control and/or inhibit insects in crop plants.
An embodiment of the present disclosure is directed towards a broad-spectrum insecticidal composition and a process for preparation thereof. The composition provides synergistic action compared to conventionally used insecticides.
In a preferred embodiment, the insecticidal composition comprises an effective amount of an isoxazoline compound and an organochlorine compound.
In one embodiment, the isooxazoline compound is fluxametamide. In one preferred embodiment of the invention, fluxametamide is present in an amount of 1 to 99%, preferably 2.5 to 5.7%, more preferably 3.8% by weight or mixture thereof.
In one embodiment, the organochlorine compound is pyridaben. In one preferred embodiment of the invention pyridaben is present in an amount of 1 to 99%, preferably 6.3 to 14.3%, more preferably 9.5% by weight or mixture thereof.
In an embodiment, the composition further comprises agrochemically acceptable excipient selected from the group consisting of, but not limited to, carrier, defoamer, wetting agent, disintegrating agent, anti-caking agent, dispersing agent, anionic emulsifier, non-ionic emulsifier sticking agent, anionic surfactant, non-ionic surfactant, polymeric surfactant, oil stabilizer, preservative, thickener, viscosity modifying agent/Rheology modifying agent, biocidal agent and anti-freezing agent and/or mixtures thereof.
The agrochemically acceptable excipients in a pre-determined ratio aids to improving the physical stability and prevents the degradation of the composition leading to long term stability and spectrum activity.
In one embodiment, insecticidal composition comprises the dispersing agent. Dispersant is a substance which adsorbs onto the surface of particles and helps to preserve the state of dispersion of the particles and prevents them from re-aggregating. Dispersants are added to agrochemical formulations to facilitate dispersion and suspension during manufacture, and to ensure the particles re-disperse into water in a spray tank. They are widely used in wettable powders; suspension concentrates and water-dispersible granules.
In a preferred embodiment dispersing agent is sodium Ligninsulphonate. In one preferred embodiment of sodium Ligninsulphonate in an amount of 2 to 5.2% by weight or mixture thereof.
In a preferred embodiment, insecticidal composition comprises the block copolymer of polyoxyethylene – polyoxypropylene glycol. These block copolymers are used as dispersants for suspension concentrates. The block copolymer of polyoxyethylene – polyoxypropylene glycol is present in an amount of 0.1 to 3% by weight or mixture thereof.
In one embodiment, insecticidal composition comprises the anti-freezing agent. In an embodiment anti-freezing agent is comprises 1,2 – Propanediol. In one preferred embodiment, 1,2 – Propanediol is present in an amount of 3.5 to 16% by weight or mixture thereof.
In another preferred embodiment, composition comprises biocidal agent. Biocides cause spoilage of formulated products. Biocidal agents are used to eliminate or reduce their effect. In an embodiment biocidal agent is Benzisothiazoline-3-one. In one preferred embodiment, Benzisothiazoline-3-one is present in an amount of 0.1% by weight or mixture thereof.
In another preferred embodiment, composition comprises defoaming agents. The presence of surfactants often causes foams to form in water-based formulations during production. The deformers are usually added to reduce the tendency to form foam, defoamers are usually added during the production stage or before bottling. In an embodiment defoaming is an emulsion of Organopolysiloxane. In one preferred embodiment, Organopolysiloxane emulsion is present in an amount of 0.05 to 0.1% by weight or mixture thereof.
In another preferred embodiment, composition comprises thickening agent. Thickeners or gelling agents are used mainly in the formulation of suspension concentrates, emulsions and suspoemulsions to modify the rheology or flow properties of the liquid and to prevent separation and settling of the dispersed particles or droplets.
In another preferred embodiment, composition comprises polysaccharide based thickening agent. In an embodiment, polysaccharide based thickening agent comprise xanthium gum. In one preferred embodiment, xanthium gum is present in an amount of 0.2 to 0.3% by weight or mixture thereof.
In another preferred embodiment, composition comprises magnesium aluminium silicate. In one preferred embodiment, magnesium aluminium silicate is present in amount of 0.1 to 0.8% by weight or mixture thereof.
In one preferred embodiment, the insecticidal composition comprises of fluxametamide, pyridaben, sodium Ligninsulphonate, block copolymer of polyoxyethylene – polyoxypropylene glycol, 1,2 – Propanediol, magnesium aluminium silicate, 1,2 -Benzisothiazoline-3-one, emulsion of organopolysiloxane, xanthium gum.
Particularly the composition utilizes fluxametamide in an amount of 1 to 99%, preferably 2.5 to 5.7%, more preferably 3.8%, pyridaben is present in an amount of 1 to 99%, preferably 6.3 to 14.3%, more preferably 9.5%, 2 to 5.2% of sodium Ligninsulphonate, 0.1 to 3% of block copolymer of polyoxyethylene – polyoxypropylene glycol, 3.5 to 16% of 1,2 – Propanediol, 0.1 – 0.8 wt% of magnesium aluminium silicate, 0.1 wt% of 1,2 -Benzisothiazoline-3-one, 0.05 – 0.1 wt% of emulsion of organopolysiloxane, 0.2 – 0.3 wt% of xanthium gum wherein water is used to make the contents up to 100%.
In another aspect, the present invention discloses a method for preparing the synergistic composition.
In an embodiment, the method of preparation comprises the following steps:
(a) Adding defoaming/dispersing agent in water with mixing to obtain a solution;
(b) Adding block copolymer and stabilizer/anti freezing agent to the solution of step (a) and mixing;
(c) Adding thickening/defoaming agent and biocidal agent to the solution of step (b) and mixing;
(d) Slowly adding around Fluxametamide and Pyridaben under continuous stirring to the solution of step (c); after addition, stir continuously to ensure proper mixing and smooth slurry formation;
(e) Mixture obtained from step (e) is milled at a temperature around 25oC;
(f) After milling, required thickening agent quantity of gel (Thick Mass) is added and stirring the slurry to get a homogeneous final formulation.
Typically, the method involves adding defoaming/dispersing agent such as sodium Ligninsulphonate in water with mixing. Adding Block copolymer of polyoxyethylene – polyoxypropylene glycol and stabilizer/anti freezing agent such as 1,2 - Propanediol and mixing. Next adding thickening/defoaming agent, Emulsion of Organopolysiloxane and biocidal agent 1,2 -Benzisothiazoline-3-one and mixing. Slowly adding around Fluxametamide and Pyridaben under continuous stirring. After addition, stir continuously to ensure proper mixing and smooth slurry formation. Mixture is milled in Dyno mill/Bead mill at a temperature around 25oC. After milling, adding required thickening agent of polysaccharide quantity of gel (Thick Mass) and stirring the slurry to get a homogeneous final formulation.
The composition can be formulated in various physical forms, for example in the form of suspension concentrate (SC) formulation, Water Dispersible Granules (WG), Wettable Powder (WP), ZC a mixed formulation of capsule suspension (CS) and suspension concentrate (SC), Suspoemulsion (SE), Oil Dispersion(OD), Flowable suspension (FS), water dispersible powder for slurry treatment (WS) or powder for dry seed treatment (DS). In one preferred embodiment of the invention, composition is in the form of a ready-to use stable liquid composition i.e., Suspension concentrate (SC).
In an embodiment, the compositions have surprisingly demonstrated that the synergistic compositions of insecticide selected from class of isooxazoline compound and class of organochlorine compound as described herein in can provide solution to the above problems.
In a preferred embodiment the insecticidal composition is used for control of insects in wide range of plants, not limited to, Tomato (Solanum lycopersicun) and Chilly (Capsicum annum). Brinjal, Cabbage, Okra, Redgram, Greenhouse cucumbers, Ornamental plants and Foliage plants grown in greenhouses and outdoors.
In another preferred embodiment, the insecticidal composition is effective in controlling the various species of leaf-eating caterpillar (Spodoptera sp), thrips (Scirtothrips dorsalis), mites (Polyphagotarsonemus latus) and fruit borer (Helicoverpa armigera), Leaf hopper, Thrips, Fruit and shoot borer, Diamond back moth, tobacco caterpillar, semilooper, Fruit Borer, Tobacco caterpillar, Spotted pod borer, pod borer, Broad mite (Family: Tarsonemidae Polyphagotarsonemus latus), False spider mite (Family: Tenuipalpidae, Brevipalpus phoenicis), Citrus flat mite (Brevipalpus lewisi), Apple rust mite (Aculus schlechtendali), Citrus bud mite (Aceria sheldoni), Citrus rust mite (Phyllocoptruta oleivora), Peach silver mite (Aculus fockeui), Pear rust mite (Epitrimerus pyri), Pink citrus rust mite (Aculops pelekassi), Citrus red mite (Panonychus citri), European red mite (Panonychus ulmi), McDaniel spider mite (Tetranychus mcdanieli), Pacific spider mite (Tetranychus pacificus), Pecan leaf scorch mite (Eotetranychus hicoriae), Sixspotted mite (Eotetranychus sexmaculatus), Southern red mite (Oligonychus ilicis), Texas citrus mite (Eutetranychus banksi), Twospotted spider mite (Tetranychus urticae), Willamette spider mite (Eotetranychus willamettei), Citrus root weevil (Pachnaeus litus), Apple aphid (Aphis pomi), Black pecan aphid (Melanocallis caryaefoliae), Black margined aphid (Monellia caryella), Brown citrus aphid (Toxoptera citricida), Yellow pecan aphid (Monelliopsis pecanis), Sweet potato whitefly (Bemisia tabaci), Silverleaf whitefly (Bemisia argentifolii), Pear Psylla (Cacopsylla pyricola), Eastern grape leafhopper (Erythroneura comes), Grape leafhopper (Erythroneura elegantula), Variegated leafhopper (Erythroneura variabilis), Virginia creeper leafhopper (Erythroneura ziczac), White apple leafhopper (Typhlocyba pomaria), Mixed formulations of Fluxematamide and Pyridaben show high synergistic activity against insect pests in terms of efficacy as well as control duration as illustrated in examples.
As required, detailed embodiments of the present invention are disclosed herein with the help of examples; however, it is to be understood that the disclosed embodiments are merely examples of the invention, which can be embodied in various forms.
Therefore, specific details disclosed herein are not to be interpreted as limiting, but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present invention. Further, the terms and phrases used herein are not intended to be limiting but rather to provide an understandable description of the invention.

EXAMPLES
To study the synergistic effect of isooxazoline compound and organochlorine compound composition/formulation comprising Fluxametamide and Pyridaben was prepared and tested for bio-efficacy in the field as over the top application on multiple crops, at different doses, and against conventionally used market standards to understand the spectrum as well as the duration of control on multiple pests. Examples 1 to 7 demonstrate the preparation of the composition.
Example 1: The composition was prepared as follows: Around 637 ltr of demineralized water was charged into a vessel (V1) fitted with anchor stirrer and continuously stirred at a low speed of 100 rpm while adding around 20 Kg of Sodium Ligninsulphonate and mixing. Then, 4 Kg of Block copolymer of polyoxyethylene – polyoxypropylene glycol and 100 Kg of 1,2 - Propanediol was added and mixed. Next 4.5 Kg of Magnesium Aluminum Silicate, 0.4 Kg of Emulsion of Organopolysiloxane and 1 Kg of 1, 2 - Benzisothiazoline - 3 – one was added and mixed. After this slowaddition of around 38 Kg pre-weighed quantity of Fluxametamide and 95 Kg of Pyridaben was done under continuous stirring. After addition, stirring was continued to ensure proper mixing and smooth slurry formation. The mixture was then milled mill in Dyno mill/Bead mill. at a temperature around 25oC to obtain a slurry. To the slurry obtained after milling, required quantity of gel (Thick Mass) around 100 Kg was added and stirred to get homogeneous final composition (Table 1).
Table 1:

Component Name

% w/w

Fluxametamide
3.80

Pyridaben
9.50
Block copolymer of polyoxyethylene – polyoxypropylene glycol
0.40

Sodium Ligninsulphonate
2.00

1,2 – Propanediol 10.00

Emulsion of Organopolysiloxane
0.05

Xanthium Gum
0.20

1, 2 - Benzisothiazoline - 3 – one 0.10

Magnesium Aluminum Silicate
0.45

Water
73.50

Total 100.0

Example 2: The composition was prepared as follows: Around 705 ltr of demineralized water was charged into a vessel (V1) fitted with anchor stirrer and continuously stirred at a low speed of 100 rpm while adding around 40 Kg of Sodium Ligninsulphonate and mixing. Then, 1 Kg of Block copolymer of polyoxyethylene – polyoxypropylene glycol and 160 Kg of 1,2 - Propanediol was added and mixed. Next 2 Kg of Magnesium Aluminum Silicate, 0.5 Kg of Emulsion of Organopolysiloxane and 1 Kg of 1, 2 - Benzisothiazoline - 3 – one was added and mixed. After this slow addition of around 25 Kg pre-weighed quantity of Fluxametamide and 62.5 Kg of Pyridaben was done under continuous stirring. After addition, stirring was continued to ensure proper mixing and smooth slurry formation. The mixture was then milled mill in Dyno mill/Bead mill. at a temperature around 25oC to obtain a slurry. To the slurry obtained after milling, required quantity of gel (Thick Mass) around 150 Kg was added and stirred to get homogeneous final composition (Table 2).
Table 2:

Component Name

% w/w

Fluxametamide
2.50

Pyridaben
6.30
Block copolymer of polyoxyethylene – polyoxypropylene glycol
0.10

Sodium Ligninsulphonate
4.00

1,2 – Propanediol 16.00

Emulsion of Organopolysiloxane
0.10

Xanthium Gum
0.30

1, 2 - Benzisothiazoline - 3 – one 0.10

Magnesium Aluminum Silicate
0.20

Water
70.50

Total 100.0

Example 3: The composition was prepared as follows: Around 739 ltr of demineralized water was charged into a vessel (V1) fitted with anchor stirrer and continuously stirred at a low speed of 100 rpm while adding around 25 Kg of Sodium Ligninsulphonate and mixing. Then, 9 Kg of Block copolymer of polyoxyethylene – polyoxypropylene glycol and 75 Kg of 1,2 - Propanediol was added and mixed. Next 1 Kg of Magnesium Aluminum Silicate, 0.5 Kg of Emulsion of Organopolysiloxane and 1 Kg of 1, 2 - Benzisothiazoline - 3 – one was added and mixed. After this slowaddition of around 42 Kg pre-weighed quantity of Fluxametamide and 105 Kg of Pyridaben was done under continuous stirring. After addition, stirring was continued to ensure proper mixing and smooth slurry formation. The mixture was then milled mill in Dyno mill/Bead mill. at a temperature around 25oC to obtain a slurry. To the slurry obtained after milling, required quantity of gel (Thick Mass) around 125 Kg was added and stirred to get homogeneous final composition (Table 3).
Table 3:

Component Name

% w/w

Fluxametamide
4.20

Pyridaben
10.50
Block copolymer of polyoxyethylene – polyoxypropylene glycol
0.90

Sodium Ligninsulphonate
2.50

1,2 – Propanediol 7.50

Emulsion of Organopolysiloxane
0.10

Xanthium Gum
0.30

1, 2 - Benzisothiazoline - 3 – one 0.10

Magnesium Aluminum Silicate
0.10

Water
73.90

Total 100.0

Example 4: The composition was prepared as follows: Around 661.1 ltr of demineralized water was charged into a vessel (V1) fitted with anchor stirrer and continuously stirred at a low speed of 100 rpm while adding around 45 Kg of Sodium Ligninsulphonate and mixing. Then, 12 Kg of Block copolymer of polyoxyethylene – polyoxypropylene glycol and 80 Kg of 1,2 - Propanediol was added and mixed. Next 6 Kg of Magnesium Aluminum Silicate, 0.5 Kg of Emulsion of Organopolysiloxane and 1 Kg of 1, 2 - Benzisothiazoline - 3 – one was added and mixed. After this slowaddition of around 55 Kg pre-weighed quantity of Fluxametamide and 137.5 Kg of Pyridaben was done under continuous stirring. After addition, stirring was continued to ensure proper mixing and smooth slurry formation. The mixture was then milled mill in Dyno mill/Bead mill. at a temperature around 25oC to obtain a slurry. To the slurry obtained after milling, required quantity of gel (Thick Mass) around 95 Kg was added and stirred to get homogeneous final composition (Table 4).
Table 4:

Component Name

% w/w

Fluxametamide
5.50

Pyridaben
13.80
Block copolymer of polyoxyethylene – polyoxypropylene glycol
1.20

Sodium Ligninsulphonate
4.50

1,2 – Propanediol 8.0

Emulsion of Organopolysiloxane
0.10

Xanthium Gum
0.20

1, 2 - Benzisothiazoline - 3 – one 0.10

Magnesium Aluminum Silicate
0.60

Water
66.11

Total 100.0

Example 5: The composition was prepared as follows: Around 707.8 ltr of demineralized water was charged into a vessel (V1) fitted with anchor stirrer and continuously stirred at a low speed of 100 rpm while adding around 30 Kg of Sodium Ligninsulphonate and mixing. Then, 22 Kg of Block copolymer of polyoxyethylene – polyoxypropylene glycol and 125 Kg of 1,2 - Propanediol was added and mixed. Next 3 Kg of Magnesium Aluminum Silicate, 0.5 Kg of Emulsion of Organopolysiloxane and 1 Kg of 1, 2 - Benzisothiazoline - 3 – one was added and mixed. After this slow addition of around 31 Kg pre-weighed quantity of Fluxametamide and 77.5 Kg of Pyridaben was done under continuous stirring. After addition, stirring was continued to ensure proper mixing and smooth slurry formation. The mixture was then milled mill in Dyno mill/Bead mill. at a temperature around 25oC to obtain a slurry. To the slurry obtained after milling, required quantity of gel (Thick Mass) around 110 Kg was added and stirred to get homogeneous final composition (Table 5).
Table 5:

Component Name

% w/w

Fluxametamide
3.10

Pyridaben
7.80
Block copolymer of polyoxyethylene – polyoxypropylene glycol
2.20

Sodium Ligninsulphonate
3.0

1,2 – Propanediol 12.5

Emulsion of Organopolysiloxane
0.10

Xanthium Gum
0.20

1, 2 - Benzisothiazoline - 3 – one 0.10

Magnesium Aluminum Silicate
0.30

Water
70.78

Total 100.0

Example 6: The composition was prepared as follows: Around 666.1 ltr of demineralized water was charged into a vessel (V1) fitted with anchor stirrer and continuously stirred at a low speed of 100 rpm while adding around 52 Kg of Sodium Ligninsulphonate and mixing. Then, 28 Kg of Block copolymer of polyoxyethylene – polyoxypropylene glycol and 140 Kg of 1,2 - Propanediol was added and mixed. Next 8 Kg of Magnesium Aluminum Silicate, 0.5 Kg of Emulsion of Organopolysiloxane and 1 Kg of 1, 2 - Benzisothiazoline - 3 – one was added and mixed. After this slow addition of around 29 Kg pre-weighed quantity of Fluxametamide and 77.5 Kg of Pyridaben was done under continuous stirring. After addition, stirring was continued to ensure proper mixing and smooth slurry formation. The mixture was then milled mill in Dyno mill/Bead mill. at a temperature around 25oC to obtain a slurry. To the slurry obtained after milling, required quantity of gel (Thick Mass) around 145 Kg was added and stirred to get homogeneous final composition (Table 6).
Table 6:

Component Name

% w/w

Fluxametamide
2.90

Pyridaben
7.30
Block copolymer of polyoxyethylene – polyoxypropylene glycol
2.80

Sodium Ligninsulphonate
5.20

1,2 – Propanediol 14.0

Emulsion of Organopolysiloxane
0.10

Xanthium Gum
0.30

1, 2 - Benzisothiazoline - 3 – one 0.10

Magnesium Aluminum Silicate
0.80

Water
66.11

Total 100.0

Example 7: The composition was prepared as follows: Around 679.6 ltr of demineralized water was charged into a vessel (V1) fitted with anchor stirrer and continuously stirred at a low speed of 100 rpm while adding around 49 Kg of Sodium Ligninsulphonate and mixing. Then, 30 Kg of Block copolymer of polyoxyethylene – polyoxypropylene glycol and 35 Kg of 1,2 - Propanediol was added and mixed. Next 3.5 Kg of Magnesium Aluminum Silicate, 0.5 Kg of Emulsion of Organopolysiloxane and 1 Kg of 1, 2 - Benzisothiazoline - 3 – one was added and mixed. After this slow addition of around 57 Kg pre-weighed quantity of Fluxametamide and 142.5 Kg of Pyridaben was done under continuous stirring. After addition, stirring was continued to ensure proper mixing and smooth slurry formation. The mixture was then milled mill in Dyno mill/Bead mill. at a temperature around 25oC to obtain a slurry. To the slurry obtained after milling, required quantity of gel (Thick Mass) around 95 Kg was added and stirred to get homogeneous final composition (Table 7).
Table 7:

Component Name

% w/w

Fluxametamide
5.70

Pyridaben
14.30
Block copolymer of polyoxyethylene – polyoxypropylene glycol
3.00

Sodium Ligninsulphonate
4.90

1,2 – Propanediol 3.50

Emulsion of Organopolysiloxane
0.10

Xanthium Gum
0.20

1, 2 - Benzisothiazoline - 3 – one 0.10

Magnesium Aluminum Silicate
0.35

Water
67.96

Total 100.0

Example 8: Performance in Field and Against Market Standards: The novel and inventive composition was tested for bio-efficacy in the field as over the top application on multiple crops, at different doses, and against market standards (currently being used by the farmers) to understand the spectrum as well as the duration of control on multiple pests. The results are shared in the Tables below and are followed by a discussion.
Table 8: Effect of the spray of inventive composition comprising Fluxametamide 3.8% + Pyridaben 9.5% SC on the incidence of leaf-eating caterpillar (Spodoptera sp) larvae, thrips, and mites on tomato during Nov. 2020 – March 2021 at Gayespur, Nadia; West Bengal.

DAT- Day after treatment, SP - Spray * Figures in the parentheses are square root (vn+1) transformed values, ROC – Reduction over control.
Results and Discussion (Table 8): A trial was conducted from Nov 2020 to March 2021, at Nadia, West Bengal on tomato, to check the efficacy of spray of composition of Fluxametamide 3.8% + Pyridaben 9.5% SC on different insects viz., Spodoptera larvae, thrips (Scirtothrips dorsalis), and mites. The formulation exhibited excellent activity against all these insects and was found statistically superior to solo Fluxametamide 10% EC, solo Pyridaben 20% WP market standards, Spiromesifen 22.9 % EC, and Lambda Cyhalothrin 5% EC up to 14 days after 2nd application. A synergy between the chemistries is evident and similar results were seen for all the three pests. Thus, the combination has been found to have broader efficacy and better synergy which will be helpful for the tomato growers where multiple pests infest the crop at the same time. The overall reduction over control was found to be in the range of 75 – 80 percent when compared with untreated control up to 14 days after 2nd spray.
Table 9: Effect of the spray of inventive composition comprising Fluxametamide 3.8% + Pyridaben 9.5% SC on the incidence of Fruit Borer (Helicoverpa armigera), thrips per leaf, and mites per leaf on chilli during Kharif 2019 – 20 at Bengaluru.

DAT- Day after treatment, SP - Spray * Figures in the parentheses are square root (vn+1) transformed values, ROC – Reduction over control.
Results and Discussion (Table 9): In a trial conducted during Kharif, 2019 – 20 at Bengaluru on chili, to check the efficacy of spray of pre-mix combination of Fluxametamide 3.8% + Pyridaben 9.5% SC on Fruit borer (Helicoverpa armigera), thrips (Scirtothrips dorsalis) and mites (Polyphagotarsonemus latus), the formulation was found to be statistically superior to solo Fluxematamide 10% EC, solo Pyridaben 20% WP, and the market standard, Emamectin benzoate 5% SG for all the three pests at 14 Days after 2nd spray. A synergy between the chemistries can be seen as is evident in the table and similar results were seen for all the three pests. Thus, the combination has been found to have broader efficacy and better synergy which will be helpful for the chili growers where multiple pests infest the crop at the same time. The overall reduction over control was found to be in the range of 88 – 90 percent when compared with untreated control up to 14 days after 2nd spray.
Advantageously, the present invention provides a composition that serves exhibits extremely useful pest controlling activity, particularly insecticidal activity, and acaricidal activity, and have no impact on non-target beings such as friendly insects, fishes, mammals etc.
The foregoing description of the invention has been set merely to illustrate the invention and is not intended to be limiting. Since the modifications of the disclosed embodiments incorporating the spirit and substance of the invention may occur to the person skilled in the art, the invention should be construed to include everything within the scope of the disclosure. ,CLAIMS:WE CLAIM:
1. An insecticidal composition comprising a) at least one insecticide selected from class of isooxazoline compound; b) at least one insecticide selected from class of organochlorine compound and one or more agrochemically acceptable excipients.

2. The insecticidal composition as claimed in claim 1, wherein isooxazoline compound is fluxametamide.

3. The insecticidal composition as claimed in claim 1, wherein organochlorine compound is pyridaben.

4. The insecticidal composition as claimed in claim 2, wherein fluxametamide is present in an amount of 1 to 99%, preferably 2.5 to 5.7%, more preferably 3.8% by weight or mixture thereof.

5. The insecticidal composition as claimed in claim 3, wherein pyridaben is present in an amount of 1 to 99%, preferably 6.3 to 14.3%, more preferably 9.5% by weight or mixture thereof.

6. The insecticidal composition as claimed in claim 1 to 5, wherein agrochemically acceptable excipients are selected from carrier, defoamer, wetting agent, disintegrating agent, anti-caking agent, dispersing agent, anionic emulsifier, non-ionic emulsifier sticking agent, anionic surfactant, non-ionic surfactant, polymeric surfactant, oil stabilizer, preservative, thickener, viscosity modifying agent/Rheology modifying agent, biocidal agent and anti-freezing agent and/or mixtures thereof.

7. The insecticidal composition as claimed in claim 1 to 6, wherein defoaming/dispersing agent comprise sodium Ligninsulphonate, preferably in an amount of 2 to 5.2% by weight or mixture thereof.

8. The insecticidal composition as claimed in claim 1 to 6, wherein anti freezing agent comprises 1,2 – Propanediol, preferably in an amount of 3.5 to 16% by weight or mixture thereof.

9. The insecticidal composition as claimed in claim 1 to 6, wherein biocidal agent is 1,2 -Benzisothiazoline-3-one, preferably in an amount of 0.1% by weight or mixture thereof.

10. The insecticidal composition as claimed in claim 1 to 6 wherein defoaming/thickening agent is an emulsion of organopolysiloxane, preferably in an amount of 0.05 to 0.1% by weight or mixture thereof.

11. The insecticidal composition as claimed in claim 1 to 6, wherein dispersing agent comprises block copolymer of polyoxyethylene – polyoxypropylene glycol, preferably in an amount of 0.1 to 3% by weight or mixture thereof.

12. The insecticidal composition as claimed in claim 1 to 6, further comprising magnesium aluminium silicate, preferably in an amount of 0.1 to 0.8% by weight or mixture thereof.

13. The insecticidal composition as claimed in claim 1 to 6, wherein thickener is selected from polysaccharide based thickening agent comprising xanthium gum, preferably in an amount of 0.2 to 0.3% by weight or mixture thereof.

14. The insecticidal composition as claimed in claim 1 to 13, wherein the composition is formulated as suspension concentrate (SC), Water Dispersible Granules (WG), Wettable Powder (WP), ZC - a mixed formulation of capsule suspension (CS) and suspension concentrate (SC), Suspoemulsion (SE), Oil Dispersion(OD), Flowable suspension (FS), water dispersible powder for slurry treatment (WS) or powder for dry seed treatment (DS), preferably Suspension concentrate (SC).

15. A process of preparation of insecticidal composition comprises steps:
(g) Adding defoaming/dispersing agent in water with mixing to obtain a solution;
(h) Adding block copolymer and stabilizer/anti-freezing agent to the solution of step (a) and mixing;
(i) Adding thickening/defoaming agent and biocidal agent to the solution of step (b) and mixing;
(j) Slowly adding Fluxametamide and Pyridaben under continuous stirring to the solution of step (c); after addition, stir continuously to ensure proper mixing and smooth slurry formation;
(k) Mixture obtained from step (d) is milled at a temperature around 25oC;
(l) After milling, required thickening agent quantity of gel (Thick Mass) is added and stirring the slurry to get a homogeneous final formulation.

Dated this 13th day of October 2022
Godrej Agrovet Limited
By their Agent & Attorney

(Adheesh Nargolkar)
of Khaitan & Co
Reg. No. IN/PA-1086