FORM2
THE PATENTS ACT, 1970
(39 of 1970)
&
The Patents Rules, 2003
COMPLETE SPECIFICATION
(See section 10; rule 13)
1. Title of the Invention.- "A SYNERGISTIC PLANT
NUTRITIONAL SUPPLEMENT FORMULATION
COMPRISING METAL GLUCONATES AND PROCESS
FOR ITS PREPARATION"
2. Applicant(s)
(a) NAME : SFPL CROP LIFE SCIENCE PRIVATE
LIMITED
(b) NATIONALITY: An Indian Company.
(c) ADDRESS: 302, Royal House, 11/3, Usha Ganj, Indore-
452001,
Madhya Pradesh, India
3. PREAMBLE TO THE DESCRIPTION
The following specification particularly describes the invention and the manner in which it is to be performed:

FIELD OF THE INVENTION
The present invention relates to the field of nutritional supplement formulation for plants. In particular, the present invention relates to nutritional mixtures which supply essential elements for preventing and correcting nutritional deficiencies in plants. The formulation essentially comprises metal gluconates and other plant nutrient supplements.The formulation has increased efficacy as it enhances the growth and yield of the treated plant as compared to the conventionally used plant nutrition agro-chemicals. The invention also provides a process for the preparation of said formulations.
BACKGROUND OF THE INVENTION
Soil micronutrient deficiency has become a deleterious threat to sustainable agricultural production and consistent crop yields under varied agro-ecological conditions. There is a need of enhancing the production of food so as to meet up the high demand of food/grains in the world.
Plants generally need 16 essential elements for growth where three of the essential nutrients i.e. carbon, hydrogen and oxygen are mainly extracted from air and water and the remaining thirteen elements i.e. calcium, magnesium, sulfur, nitrogen, phosphorous, potassium, iron, manganese, copper, zinc, molybdenum, chlorine and boron must be absorbed through the roots from the surrounding soil (as known from literature; "Principles of plant nutrition" Konrad Mengel and Ernest A. Kirkby Ed. Kluvert Academic Publishers). Each of these sixteen nutrients is indispensable for the growth, development and overall health of plants.
The introduction of hybrids through plant breeding and green revolution in addition to adoption of GM technology has increased the demand for use of agri-inputs in standard agriculture practices. Similarly, intensive crop cultivation and imbalanced use of fertilizers have led to serious depletion of both macro and micronutrients from the soil throughout the globe. The analysis of 2.52 lakhs surface soil samples collected from different parts of India revealed the predominance of zinc deficiency in divergent soils. About 49, 12, 4, 3, 33% and

41% of these soils are tested to be deficient in available zinc, iron, manganese, copper, boron and sulphur, respectively. Plants generally require at least 14 mineral nutrients for adequate nutrition in addition to Oxygen, carbon dioxide and water. It is very important to provide plants with a balanced fertilizer nutrition program containing macro and micronutrients for the production of better quality and sustainable higher yields. The deficiency of any one of these essential mineral elements in required quantities can lead to dramatic changes in the physiological and metabolic processes of the plant thereby resulting in reduced vegetative growth and yield.
Presently the nutrient deficiencies are corrected by use of chemical based products like Ethylenediamine tetraacetic acid (EDTA) and inorganic salts. Plants are not able to absorb and assimilate inorganic salt based plant nutrients and to meet the nutritional requirement of plants large scale application of chemicals is needed which is known to pollute and degree the soil. EDTA is a polyamino carboxylic acid which is a colourless and water soluble compound. It is a chelate ligand with a high affinity constant to form metal-EDTA complexes. The widespread use of EDTA in fertilizers and the slow decomposition of this compound have led to background concentrations of EDTA in some European surface waters in the range of 10 to 50 mg L-1. In addition to that, these compounds when applied are not absorbed by the plants so they are left behind in the soil and build up over time emerging as a persistent organic pollutant. The presence of these compounds in large amounts in soils can alter the soil chemistry and makes it less ideal for planting. EDTA degrades to ethylenediaminetriacetic acid, which then cyclizes to the diketopiperizide, a cumulative, persistent, organic environmental pollutant. It has been found to be both cytotoxic and weakly genotoxic in laboratory animals. Oral exposures of this compound have been noted to cause reproductive and developmental effects in animals. Many synthetic chelates and their complexes with heavy metals are toxic and poorly photo, chemo, and biodegradable in soil environments. Studies conducted earlier have also shown that many chelates including EDTA are toxic for both plants and soil microorganisms and pose a

considerable environmental problem due to their leaching towards groundwater. The resistance of EDTA to bacterial biodegradation is widely documented and the compound is harmful to gram negative bacteria by causing destruction of bacterial outer membrane. It is also toxic to photosynthetic organisms and inhibits their cellular division, chlorophyll synthesis and algal biomass production. US 20050256091 relates to cupric salts of derivatives of salicylic acid and their application for the control of phytopathogens. Several microbial based bioproducts are commercially available and being used in the agriculture but the limitations of these products is in their composition and in their application to a particular crop. Some beneficial organisms are effective in the laboratory only, but do not show their activity in the field, even after development of a product for market. Prior to the application, too little active material actually reaches to the field for application and rapid degradation occurs in the field. Formulation of a bioinoculant plays a vital role in helping to solve these problems and in making available critical numbers of organism for application in the field. US Pat. No. 5,697,186 discloses the use of microorganisms to enhance crop productivity and, more specifically, to the use of flocculated forms of bacteria, particularly Azospirillum and Rhizobium, or a combination thereof, as crop inoculants and delivery systems for other agriculturally beneficial microorganisms.
US Pat. No. 7,097,830 discloses synergistic bioinoculant composition comprising Bacillus strains isolated from cows, either individually or in all possible combinations, and optionally a carrier, with each of the strains showing plant growth-promoting activity. Most of the time, these products either contain only one plant growth property. So for getting multiple benefits, the farmers have to apply best choice of products. Therefore, for better cropping practices it is desirable to develop a bio-product with multiple properties which can be used alone.
Thus, there exists need of a cost effective 100% organic plant nutrition supplement formulation which are capable of increasing the growth and yield of crop plants higher than the conventionally used plant nutrition agro-chemicals.

Further, there is also a need of a plant nutrition supplement formulations which are quick in action, highly stable at normal storage, compatible with most of the agro-chemicals and safe to human, animals and environment.
OBJECT OF THE INVENTION
The primary object of the invention is to overcome the drawbacks mentioned in the prior art.
Another object of the present invention is to provide a plant nutritional supplement formulation comprising metal gluconates which enhances the growth and yield of the treated plant as compared to the conventionally used plant nutrition agro-chemicals treated plants.
Another object of the present invention is to provide a plant nutritional supplement formulation which is highly stable at normal storage for a longer life of more than 3 years.
Another object of the present invention is to provide a plant nutritional supplement formulation which is 100% organic in nature, quick effective, compatible with most of the agro-chemicals and safe to human, animals and environment.
Another object of the present invention is to provide a process for the production of plant nutritional supplement formulation comprising metal gluconates and nutrition supplements.
Another object of the present invention is to provide a process for the production of other derived metal gluconates from calcium gluconate.
These and other advantages of the present invention will be more apparent from the foregoing description in conjunction with the accompanying drawings.
SUMMARY OF THE INVENTION
The following presents a simplified summary of the invention in order to provide

a basic understanding of some aspects of the invention. This summary is not an extensive overview of the present invention. It is not intended to identify the key/critical elements of the invention or to delineate the scope of the invention. Its sole purpose is to present some concept of the invention in a simplified form as a prelude to a more detailed description of the invention presented later.
The invention provides a plant nutrition supplement formulation comprising metal gluconate(s) and plant nutrition supplements wherein said metal gluconate is present in an amount ranging from 18 to 36%, preferably around 24% and each of the plant nutrition supplement is present in an amount ranging from 0.50 to 5.0%, preferably around 1%.
Also, the invention provides a process for the production of plant nutrition supplement formulation comprising:
a) reaction of a calcium gluconate with an acid to produce gluconic acid;
b) reaction of gluconic acid produced in step (a) with metal oxide to produce corresponding metal gluconate;
c) optionally, reacting calcium gluconate with boric acid to produce calcium borogluconate;
d) reaction of metal gluconate(s) produced in step (b) or (c) or combination thereof with plant nutrient supplement to produce said formulation along with stirring the solution for approximately 1 hour at approximately 60 rpm.
The invention furthermore provides a process for the preparation of metal gluconate(s) using calcium gluconate as the raw material.
DETAILED DESCRIPTION OF THE INVENTION
The following description is of exemplary embodiments only and is not intended to limit the scope, applicability or configuration of the invention in any way. Rather, the following description provides a convenient illustration for implementing exemplary embodiments of the invention. Various changes to the

described embodiments may be made in the function and arrangement of the elements described without departing from the scope of the invention.
The present invention in the main aspect provides a synergistic plant nutrient supplemental formulation which is 100% organic in nature, quick effective and is efficient in enhancing the growth and yield of the treated plant as compared to the conventionally used plant nutrition agro-chemicals treated plants.
The formulation essentially comprising metal gluconate(s) and plant nutrition supplements. The metal gluconate is present in the range of 18% to 36%, preferably around 24% () while each of the plant nutrition supplements is present in an amount ranging from 0.5 to 5% and preferably around 1% The metal gluconate is selected from a group comprising of magnesium gluconate, ferrous gluconate, calcium borogluconate, manganese gluconate, potassium gluconate, zinc gluconate or combination thereof. The plant nutrient supplement comprises amino acid, sea weed, humic, fulvic, ascorbic acid or combinations thereof.
PRODUCTION OF METAL GLUCONATES
In the present invention, calcium gluconate is used as a raw material to formulate various novel synergistic formulations of gluconates and organic plant nutrition supplements which significantly increases crops growth & yield higher as compared to the conventionally used plant nutrition agro-chemicals. The calcium gluconate at first step is processed to produce various other derived metal gluconates like magnesium gluconate, ferrous gluconate, calcium borogluconate, zinc gluconate, manganese gluconate, potassium gluconate etc.
All the metal Gluconates like Magnesium Gluconate, Potassium Gluconate, Potassium Gluconate, Zinc Gluconate, Copper Gluconate and Ferrous Gluconate could be produced by converting Calcium Gluconate into Gluconic acid by reacting it with sulfuric acid and further adding metal oxide to it.

However, Calcium Boro Gluconate could be produced directly without making Gluconic acid.
Further, a metal gluconate or even mixtures of two or more gluconates are formulated with the organic plant nutrition supplements for the production of final synergistic plant nutritional supplement formulation.
The below stepwise process illustrates the process for the production of other metal gluconates from calcium gluconate
Following are the stepwise reactions for the production of metal gluconate formulation:
• Step 1:
Calcium Gluconate (M) + Sulfuric Acid (M) Gluconic Acid (2M)
• Step 2:
Gluconic Acid (2M) + X Metal Oxide X Metal gluconate
• Step 3:
X Metal gluconate + plant nutrition supplements Final formulation
Following are the stepwise reactions for the production of mixture of metal gluconate formulation:
• Step 1:
Calcium Gluconate (M) + Sulfuric Acid (M) Gluconic Acid (2M)
• Step 2:
Gluconic Acid (2M) + Y Metal Oxide Y Metal gluconate
• Step 3:
Gluconic Acid (2M) + Z Metal Oxide Z Metal gluconate
• Step 4:
Y Metal gluconate + Z Metal gluconate + plant nutrition supplements Final
formulation

The process of producing various synergistic formulations comprising metal gluconates and plant nutrition supplements are detailed below:
PRODUCTION OF MAGNESIUM GLUCONATE & PLANT NUTRITION SUPPLEMENTS FORMULATION
• Step 1:
Calcium Gluconate (M) +• Sulfuric Acid (M) Gluconic Acid (2M)
• Step 2:
Gluconic Acid (2M) + Magnesium Oxide (M) Magnesium Gluconate
• Step 3:
Magnesium Gluconate (24%) + Amino (1%) +Final Formulation
Seaweeds (1%) + Humic (1%) + Fulvic (1%) + (MGF)
Ascorbic Acid (1%)
Magnesium gluconate was mixed with various ingredients of plant nutrition supplements (amino acid, seaweeds, humic, fulvic & ascorbic acid) and stirred for 1 hour at 60 RPM. The final formulations (MGF) are highly stable with longer shelf life of more than 3 years.
PRODUCTION OF MANGANESE GLUCONATE & PLANT NUTRITION SUPPLEMENTS FORMULATION
1. Calcium Gluconate (M) + Sulfuric Acid (M) Gluconic Acid (2M)
2. Gluconic Acid (2M) + Manganese Oxide (M) Manganese Gluconate
(M)
3. Manganese Gluconate (24%) + Amino (1%)Final Formulation
+ Seaweed (1%) + Humic (1%) + Fulvic (1%) (MnGF)
+ Ascorbic Acid (1%)
Manganese gluconate was mixed with various ingredients of plant nutrition supplements (amino acid, seaweed, humic, fulvic & ascorbic acid) and stirred for 1 hour at 60 RPM. The final formulations (MnGF) are highly stable with longer

shelf life of more than 3 years.
PRODUCTION OF ZINC GLUCONATE & PLANT NUTRITION SUPPLEMENTS FORMULATION
1. Calcium Gluconate (M) + Sulfuric Acid (M) Gluconic Acid (2M)
2. Gluconic Acid (2M) + Zinc Oxide (M) Zinc Gluconate (M)
3. Zinc Gluconate (24%) + Amino (1%) + Seaweed Final Formulation (1%) + Humic (1%) + Fulvic (1%) + Ascorbic Acid (1%) (ZGF)
Zinc gluconate was mixed with various ingredients of plant nutrition supplements (amino acid, seaweed, humic, fulvic & ascorbic acid) and stirred for 1 hour at 60 RPM. The final formulations (ZGF) are highly stable with longer shelf life of more than 3 years.
PRODUCTION OF GLUCONATE MIXTURE & PLANT NUTRITION SUPPLEMENTS FORMULATION
1. Calcium Gluconate (M) + Sulfuric Acid (M) Gluconic Acid (2M)
2. Gluconic Acid (2M) + Zinc Oxide (M) Zinc Gluconate (M)
3. Gluconic Acid (2M) + Magnesium Oxide (M) Magnesium
Gluconate (M)
4. Gluconic Acid (2M) + Manganese Oxide (M) Manganese Gluconate
(M)
5. Gluconic Acid (2M) + Ferrous Oxide (M) Ferrous Gluconate (M)
6. Zinc Gluconate (%) + Magnesium Gluconate (%) + Manganese Gluconate (%) + Ferrous Gluconate (%) + Boric Acid (%) + Sodium Molybdate (%) + Amino (1%) + Seaweed (1%) + Humic (1%) + Fulvic (1%) + Ascorbic Acid (1%)
Final Formulation (GMF) Zinc gluconate, Magnesium Gluconate, Manganese Gluconate, Ferrous Gluconate, Boric Acid, Sodium Molybdate and plant nutrition supplements (amino acid, seaweed, humic, fulvic & ascorbic acid) were added and stirred for 1

life of more than 3 years.
The developed synergistic formulations of metal gluconates and plant nutrient supplements having following advantages:
• 100% organic in nature,
• quick effective,
• Highly stable at normal storage with longer shelf life of more than 3 years.
• compatible with most of the agro-chemicals
• Safe to human, animals and environment.
Humic, fulvic and amino may be used either in natural form or in the form of chemical derivatives or salts. It is stated that the metal gluconates i.e. magnesium gluconate, ferrous gluconate, calcium boro gluconate, manganese gluconate, potassium gluconate and zinc gluconate may even be obtained from the commercial market for using them for the production of said plant nutrient formulation. However, it is noteworthy that the metal gluconates prepared in the laboratory is preferred over those present commercially in the market so as to ensure the quality of the final supplement formulation.
Further, it will be appreciated by those of skill in the art that proportions of various plants extracts used in the above formulations may be increased or decreased in accord with the present invention. In many instances, formulations including fewer than all of the above listed ingredients will be effective. The non-toxic surfactants of natural origin can be applied in the formulation for its better coverage and spray. The formulation of the present invention have been found to be quick effective, safe to human, animals, environment, suitable for foliar application and is cost effective. As discussed above various modifications and substitutions to the formulations of the present invention will be readily apparent to one of skill in the art

EXPERIMENTAL DATA TO SHOW SYNERGISTIC EFFECT OF THE
PLANT NUTRIENT SUPPLEMENT FORMULATION OF PRESENT
INVENTION
Field Evaluation of the Synergistic Final Formulations of Gluconates and
Plant Nutrition Supplements:
Field trials were conducted to evaluate the performance of the developed
formulations on various economically important crops. Details of the trials
conducted on various crops are given below:
TRIAL 1: The main objective of this trial was to optimize the application rates of MGF in Cotton. The trial was conducted in-house at Gundewadi, SFPL for two seasons.
The trial was conducted in RBD and it consisted of 7 treatments which were replicated 3 times. The formulations (MGF) were sprayed two times during the growing season (55 & 85 days after sowing - DAS). Observations were recorded periodically up to harvest.
TABLE 1: EFFECT OF DIFFERENT RATES OF MAGNESIUM GLUCONATE FORMULATION (MGF) ON GROWTH AND YIELD OF COTTON AT GUNDEWADI, JALNA

Treatme nts Boll
Number
plant-1 % IOC Boll
Weigh
t(g) % IOC Plant height
(cm) % IOC Number
of sympodia % IOC Final plant stand plot-1 % IOC Yield (Qha-1) % IOC
Control 52.66 - 4.57 - 129.67 - 20.27 - 35.36 - 22.18 -
MGF
(0.5 ml/liter) 54.46 3.42 4.93 7.88 129.67 0 21.00 3.6 35.36 0 22.84 2.98
MGF
(1 ml/liter) 56.92 8.09 4.87 6.56 135.67 4.63 22.02 8.63 35.50 0.4 24.96 12.53
MGF
(2 ml/liter) 59.89 13.73 4.87 6.56 140.67 8.48 24.54 21.07 35.50 0.4 26.41 19.07
MGF
(3 ml/liter) 79.47 50.91 4.83 5.69 147.00 13.3 6 24.67 21.71 35.83 1.33 37.21 68.67
MGF 82.57 56.8 4.97 8.75 153.67 18.5 25.88 27.68 35.84 1.36 41.04 85.03

(4 ml/liter) 1
MGF
(5 ml/liter) 92.41 75.48 4.97 8.75 160.67 23.9 1 27.44 35.37 36.50 3.22 46.59 110.05
* Data presented in the table is an average of two seasons of field trials conducted on Cotton at Jalna
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). The results of the trial showed that the maximum improvement in boll number, weight and cotton yield was recorded when the product was added at the rate of 3 ml or 4 ml/litre of water. To get maximum benefit gluconates should be applied at the rate of 5ml/L of water.
TRIAL 2: The trial was conducted to determine the best stages of Gluco Magnesium application in cotton for three seasons
The trial was conducted in RBD and it consisted of 7 treatments which were replicated 3 times. The formulations (MGF) were sprayed at different times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 2: EFFECT OF APPLICATION OF MAGNESIUM GLUCONATE FORMULATION (MGF) AT DIFFERENT STAGES ON GROWTH AND YIELD OF COTTON

Treatment Boll
Number
plant-1 % IOC Boll Weight
(g) % IOC Plant
height
(cm) % IOC Number
of sympodi
a % IOC Final plant stand plot"1 % IOC Yield (Qha-1) % IOC
Control 66.28 - 4.78 - 126.67 - 18.94 - 35.67 - 33.4 -
MGF spray at 55 days 71.92 8.51 5.08 6.28 133.22 5.17 20.33 7.34 36.22 1.54 35.45 6.14
MGF spray at 75 days 68.98 4.07 5.06 5.86 135.11 6.66 20.47 8.08 36.55 2.47 34.29 2.66
MGF spray at 90 days 71.06 7.21 5.06 5.86 135 6.58 21.36 12.78 37.11 4.04 34.93 4.58
MGF spray 76.78 15.84 5.04 5.44 146.89 15.96 23.92 26.29 37.56 5.3 37.93 13.56

at 55 and 75 days
MGF spray at 55 and 90 days 80.27 21.11 5.09 6.49 155.33 22.63 26.13 37.96 37.67 5.61 42.24 26.47
MGF spray
at 55, 75 and 90 days 79.96 20.64 5.07 6.07 149.78 18.24 24.03 26.87 38.22 7.15 40.77 22.07
* Data presented in the table is an average of three seasons of field tna s conducted on Cotton at Jalna
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). The present trial revealed that the best results on boll number, boll weight and cotton yield was recorded when MGF application was done at square initiation (55 DAS) and fruiting stages (90 DAS) in cotton. Beneficial effects of MGF in Cotton are more when it is applied in these two stages.
TRIAL 3: Field trials were conducted at MAU, Parbhani to compare the efficacy of various gluconate based formulations and EDTA Chemical based agri-input products in improving the growth and yield in Cotton.
The trial was conducted in RBD and it consisted of 16 treatments which were replicated 2 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 3: EFFECT OF DIFFERENT GLUCONATE AND EDTA PRODUCTS ON GROWTH AND YIELD PARAMETERS OF COTTON

Treatments Kharif 2009 Kharif 2010

Boll Number % IOC Seed Yield (Qha-1) % IOC Boll Number % IOC Seed Yield (Qha-1) % IOC
Control 37.25 0 18.05 0 34.5 0 17.38 0
ZGF 45.25 21.48 27.29 51.9 41 18.84 19.35 11.33
Zinc EDTA 38.25 2.68 23.52 30.3 36.5 5.8 16.55 0

MnGF 48.76 30.9 24.22 34.18 37.5 8.7 14.1 0
Manganese EDTA 49.75 33.56 19.72 9.25 29.75 0 16.27 0
CuGF 45 20.81 19.31 6.98 35.5 2.9 18.38 5.75
Copper EDTA 42.25 13.42 14.31 0 33.75 0 21.72 24.97
FeGF 50 34.23 24.83 37.56 42.5 23.19 22.48 29.4
Ferrous EDTA 41.75 12.08 24.62 36.4 37 7.25 19.58 12.66
CaGF 42.75 14.77 21.22 17.56 28 0 17.47 0.52
Calcium EDTA 48.75 30.87 20.62 14.24 39 13.04 17.96 3.34
MGF 53.25 42.95 24.33 34.79 45.5 31.88 23.9 37.51
Magnesium EDTA 45.5 22.15 22.06 22.22 41.25 19.57 21.5 23.71
GMF 36 0 21.76 20.55 29.5 0 18.62 7.13
All EDTA 30.75 0 16.69 0 28 0 19.85 14.21
Chemicals
(Grade 2) 36.5 0 21.76 20.55 27.5 0 20.32 16.92
* Data presented in the table is a mean of two replicates (ZGF = Zinc Gluconate Formulation, MnGF = Manganese Gluconate Formulation, CuGF = Copper Gluconate Formulation, FeGF = Ferrous Gluconate Formulation, CaGF = Calcium Gluconate Formulation, MGF = Magnesium Gluconate Formulation, GMF = Gluconate Mix Formulation)
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). In both seasons, the best results were recorded in gluconate applied plots compared to EDTA applied ones. MGF, FeGF and ZGF performed better than other gluconates and all EDTA based products in Cotton. Foliar application of gluconates can be a suitable alternate to EDTA based products in Cotton.
TRIAL 4: Trial was conducted at Ghanewadi, MS to test the efficacy of Calcium Boro Gluconate Formulation (CBGF), ZGF, Potassium Gluconate Formulation (PGF) and MGF on the growth and yield parameters of Cotton.

The trial was conducted in RBD and it consisted of 5 treatments which were replicated 5 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 4: EFFICACY OF VARIOUS GLUCONATE PRODUCTS ON THE GROWTH AND YIELD PARAMETERS OF COTTON

Treatme nt Plant height
(cm) % IOC Number
of monopo
dia % IOC Number of sympodia % IOC Number of bolls plant" % IOC Boll weight
(g) % IOC Yield (Qha-1) % IOC
Control 159.22 - 2.46 - 19.66 - 66.52 - 4.51 - 22.24 -
CBGF 162.46 2.03 2.64 7.32 20.84 6 68.38 2.8 4.64 2.88 24.06 8.18
PGF 163.98 2.99 2.72 10.57 21.32 8.44 70.32 5.71 4.72 4.66 24.62 10.7
MGF 170.3 6.96 2.8 13.82 22.18 12.82 73.54 10.55 4.86 7.76 25.78 15.89
ZGF 175.54 10.25 2.98 21.14 23.76 20.85 76.52 15.03 5.03 11.53 26.76 20.32
* Data presented in the table is a mean of five replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). Among various gluconates, the best effects in boll number, boll weight and yield was recorded by foliar application of ZGF and MGF. PGF and CBGF also improved the growth and yield parameters of Cotton but were not as good as ZGF and MGF. ZGF and MGF could be used as a substitute for Zinc sulphate/ Zinc EDTA and Magnesium sulphate/ Magnesium EDTA, respectively in Cotton.
EFFECT OF GLUCONATE BASED FORMULATIONS ON MAIZE
Trial 5: Trial was conducted at Ghanewadi, MS to test the efficacy of CBGF,
ZGF, PGF and MGF on the growth and yield parameters of Maize.
The trial was conducted in RBD and it consisted of 5 treatments which were replicated 5 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest. TABLE 5: EFFICACY OF VARIOUS GLUCONATE PRODUCTS ON THE

GROWTH AND YIELD PARAMETERS OF MAIZE IN GHANEWADI

Treatme
nts Plant height (cm) % IOC Root
length
(cm) % IOC Number
of grains
row-1 % IOC Number of rows cob-1 % IOC Grain
Yield (Q
ha1) % IOC
Control 218.6 - 21.7 - 37.20 - 16.08 - 95.95 -
CBGF 221.9 1.51 23.5 8.29 39.24 5.48 16.40 1.99 99.35 3.54
PGF 223.8 2.38 23.5 8.29 39.18 5.32 16.28 1.24 100.70 4.95
MGF 224.9 2.88 24.8 14.29 39.28 5.59 16.36 1.74 102.50 6.83
ZGF 226.2 3.48 25.5 17.51 39.62 6.51 16.66 3.61 104.00 8.39
* Data presented in the table is a mean of five replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). The best height on plant height, grain number and yield were recorded by foliar application of ZGF and MGF. Although the results recorded by PGF and CBGF in Maize were good, they were not as good as ZGF and MGF.
ZGF and MGF can be recommended for use in Maize crop production as an alternative to EDTA and other chemical based Zinc and Magnesium based nutrition products.
EFFECT OF GLUCONATE BASED FORMULATIONS ON WHEAT TRIAL 6: Trial was carried out to compare the efficacy of gluconate and EDTA based products in improving wheat yield.
The trial was conducted in RBD and it consisted of 7 treatments which were replicated 3 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. The observation related to yield was recorded after harvest.
TABLE 6: EFFECT OF DIFFERENT GLUCONATE AND EDTA PRODUCTS ON YIELD OF WHEAT

Treatments Yield (Q/ha) % IOC
Control 29.87 -
ZGF 36.69 22.83

Zinc EDTA 30.23 1.21
MnGF 32.07 7.37
Manganese EDTA 31.20 4.45
Chemical Grade 2 30.49 2.07
GMF 33.67 12.9
* Data presented in the table is a mean of three replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). ZGF performed better than all other tested products in improving wheat yield. Gluconate based products generally improved wheat yield compared to their EDTA counterparts.
TRIAL 7: Trial was conducted to optimize the application rates of granular ZGF and PGF in Wheat.
The trial was conducted in RBD and it consisted of 12 treatments which were replicated 3 times. The solid formulations were applied at the time of sowing. Observations were recorded periodically up to harvest.
TABLE 7: EFFECT OF DIFFERENT DOSAGE LEVELS OF GRANULAR ZGF AND PGF ON GROWTH AND YIELD PARAMETERS OF WHEAT

Treatments Plant height
(cm) % IOC Ear length
(cm) % IOC Number of
spikelets/
ear % IOC Number
of grains/
ear % IOC Grain Yield
(Qha-1) % IOC
Control I 76.9 - 7.22 - 11.6 - 29.2 - 26.75 -
ZGFSolid (10 Kg/ha) 87.9 14.3 10.2 41.27 20.2 74.14 50 71.23 39.75 48.6
ZGFSolid (20
Kg/ha) 92.6 20.42 9.72 34.63 15.8 36.2 51.6 76.71 36.5 36.45
ZGFSolid (30 Kg/ha) 90.6 17.82 10.26 42.11 16.2 39.65 48 64.38 34 27.1
ZGFSolid (40 Kg/ha) 87.2 13.39 8.7 20.5 12.75 9.91 36.5 25 31.5 17.76
ZGFSolid (50 Kg/ha) 85.3 10.92 9.4 30.19 18.4 58.62 42.2 44.52 32 19.63

Control II 81.8 - 9.6 - 15.4 - 46.8 - 25 -
PGFSolid (10 Kg/ha) 90.3 10.39 10.22 6.46 16.8 9.09 55.2 17.95 42.25 69
PGFSolid (20 Kg/ha) 89.2 9.05 9.4 0 14.8 0 47.8 2.14 32.88 31.5
PGFSolid (30 Kg/ha) 87.3 6.72 9.44 0 15.4 0 43 0 32.5 30
PGFSolid (40
Kg/ha) 87.1 6.48 10.42 8.54 15.6 1.29 41.8 0 31.75 27
PGFSolid (50
Kg/ha) 85.9 5.01 11.38 18.54 17.2 11.69 53.8 28.71 39.75 59
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). The best results of ZGF Solid and PGF Solid improving wheat growth and yield was obtained when ZGF Solid and PGF Solid were applied at the rate of 10 Kg/ha. Application of ZGF Solid and PGF Solid granules at the rate of 10 Kg/ha can be recommended to the farmers. The one advantage of using granular Gluconates is that it can be applied along with the regular fertilizers at the time of sowing which will save time and labour.
TRIAL 8: Trial was conducted to compare the efficacy of ZGF with other available agri-input Zinc products in improving the growth and yield of Wheat.
The trial was conducted in RBD and it consisted of 4 treatments which were replicated 5 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 8: EFFICACY OF ZGF IN COMPARISON WITH OTHER AVAILABLE AGRI-INPUT ZINC PRODUCTS IN IMPROVING THE GROWTH AND YIELD PARAMETERS OF WHEAT

Treatment Plant height % Ear length % Number % IOC Number % Grain % IOC
(cm) IOC (cm) IOC of
spikelets/
ear of grains/ ear IOC Yield (Qha-1)

Control
81.84 - 9.64 - 14.64 - 53.2 - 25.56 -
ZGF 84.88 3.71 10.48 8.71 16.6 13.39 57.28 7.67 26.67 4.35
Zinc EDTA 84.8 3.62 10.6 9.96 16.84 15.03 57.76 8.57 26.33 3.04
Zinc Sulphate 84.68 3.47 9.85 2.28 15.2 3.83 57.12 7.37 25.78 0.87
* Data presented in the table is a mean of five replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). Gluco Zinc performed better than Zinc EDTA and Zinc sulphate in improving the growth and yield of Wheat. Gluco Zinc can be used as a substitute for Zinc EDTA and Zinc Sulphate in Wheat as it is organic, eco-friendly in nature and improves yield.
EFFECT OF GLUCONATE BASED FORMULATIONS ON SOYBEAN TRIAL 9: Trial was conducted to test the efficacy of ZGF, FeGF, PGF and MGF on growth and yield parameters of Soybean.
The trial was conducted in RBD and it consisted of 5 treatments which were replicated 5 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 9: EFFICACY OF VARIOUS GLUCONATE PRODUCTS ON THE GROWTH AND YIELD PARAMETERS OF SOYBEAN

Treatments Plant height
(cm) % IOC Root length (cm) % IOC Number of pods plant-1 % IOC Number of seeds pod-1 % IOC Yield (Qha-1) %IC
Control 71.84 - 15.16 - 41.8 - 2.48 - 19.64 -
FeGF 78.16 8.8 15.62 3.03 50.24 20.19 2.69 8.47 21.27 8.3
PGF 73.84 2.78 15.36 1.32 45.36 8.52 2.63 6.05 20.34 3.5
MGF 75.00 4.41 15.70 3.56 46.96 12.34 2.66 7.26 20.60 4.8
ZGF 81.80 13.86 15.92 5.01 51.28 22.68 2.73 10.08 21.54 9.6
* Data presented in the table is a mean of five replicates

The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). Gluco Zinc and Gluco Ferrous performed better than other gluconates in improving the growth and yield parameters in Soybean. Gluco Zinc and Gluco Ferrous can be used as a substitute for Zinc Sulphate/ Zinc EDTA and Ferrous Sulphate/ Ferrous EDTA in Soybeart.
EFFECT OF GLUCONATE BASED FORMULATIONS ON CHILLI TRIAL 10: Trial was conducted at Ghanewadi, MS to test the efficacy of ZGF, FeGF, PGF and MGF on growth and yield parameters of Chilli.
The trial was conducted in RBD and it consisted of 5 treatments which were replicated 5 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 10: EFFICACY OF VARIOUS GLUCONATE PRODUCTS ON THE GROWTH AND YIELD PARAMETERS OF CHILLI

Treatmen ts Plant
height
(cm) % IOC Number of fruits % IOC Fruit length
(cm) % IOC Fruit girth (cm) % IOC Fruit
weight (gm
plant1) % IOC Yield
(t ha-1) % IOC
Control 89.26 - 102.40 - 10.05 - 0.63 - 563.3 - 16.69 -
FeGF 99.50 11.47 108.20 5.66 10.71 6.57 0.71 12.7 609.1 8.13 18.04 8.09
PGF 94.28 5.62 105.38 2.91 10.57 5.17 0.74 17.46 590.4 4.81 17.49 4.79
MGF 94.12 5.44 105.42 2.95 10.62 5.67 0.74 17.46 592.3 5.15 17.55 5.15
ZGF 100.70 12.82 108.90 635 10.80 7.46 0.79 25.4 619.7 10.01 18.36 10.0
* Data presented in the table is a mean ot five replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). ZGF and FeGF performed better than other gluconates in improving the growth and yield parameters in Chilli. ZGf andd FeGF can be used as a substitute for Zinc Sulphate/ Zinc EDTA and Ferrous Sulphate/ Ferrous EDTA in Chilli.
EFFECT OF GLUCONATE RASED FORMULATIONS ON ONION

TRIAL 11: Trial was conducted during to test the efficacy of seeds coated with
ZGF on growth and yield parameters of Onion.
The seeds of onion were encrusted with ZGF @ 20ml/kg of seed, dried in shade
and used for field trials. The trial was conducted in RBD and it consisted of 2
treatments which were replicated 12 times. Observations were recorded
periodically up to harvest.
TABLE 11: EFFECT OF SEEDS COATED WITH ZGF ON GROWTH AND
YIELD PARAMETERS OF ONION

Treatmen ts Plant
height
(cm) % IOC Neck thickness
(cm) % IOC Bulb Weight
(g) % IOC Bulb diameter
(cm) % IOC Yield
(t/ha) % IOC
Control 54.03 - 1.12 - 122.7 - 6.08 - 22.43 -
ZGF 59.8 10.69 1.32 17.67 151.28 23.29 7.04 15.84 27.59 22.9
* Data presented in the table is a mean ol 12 replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). ZGF improved the growth and yield parameters of Onion compared to Control. Seeds encrusted with gluconates will improve the growth and yield in Onion plants. Use of Onion seeds encrusted with gluconates is profitable and beneficial to the farming community.
EFFECT OF GLUCONATE BASED FORMULATIONS ON TOMATO TRIAL 12: Field trial was conducted to compare the efficacy of gluconate, EDTA and sulphate based Zinc products in improving the growth and yield in Tomato.
The trial was conducted in RBD and it consisted of 10 treatments which were replicated 3 times. The different formulations were applied as per treatment requirement. Observations were recorded periodically up to harvest. TABLE 12: EFFECT OF DIFFERENT METHODS OF APPLICATION OF VARIOUS ZINC PRODUCTS ON THE GROWTH AND YIELD OF TOMATO

Treatments Plant height % IOC Fruit % IOC Fruit % IOC Fruit % IOC

(cm) Number /plant Weight/plant
(g) yield
(t/ha)
Control 69.66 0 21.66 0 1.21 0 41.39 -
ZGF
(Foliar) 90.00 29.2 47.33 118.51 1.58 30.58 57.78 39.60
ZGF (Soil) 94.66 35.89 49.66 129.27 1.66 37.19 58.70 41.82
Zinc EDTA (Foliar) 86.33 23.93 37.00 70.82 1.47 21.49 54.69 32.13
Zinc EDTA (Soil) 84.66 21.53 41.00 89.29 1.51 24.79 55.95 35.18
Zinc
Sulphate
(Foliar) 80.33 15.32 25.00 15.42 1.27 4.96 47.06 13.70
Zinc Sulphate
(Soil) 77.66 11.48 28.66 32.32 1.35 11.57 50.08 21.00
ZGF (Soil + Foliar) 93.33 33.98 51.33 136.98 1.85 52.89 63.26 52.84
Zinc EDTA
(Soil + Foliar) 84.33 21.06 42.33 95.43 1.52 25.62 56.46 36.41
Zinc Sulphate
(SoiI + Foliar) 84.00 20.59 34.00 56.97 1.39 14.88 51.56 24.57
* Data presented in the table is a mean of three replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). The foliar and soil application of ZGF performed better than foliar and soil applications of Zinc EDTA and Zinc Sulphate in improving the growth and yield parameters of Tomato. ZGF can be used as a substitute for Zinc sulphate/ Zinc EDTA in Tomato.
EFFECT OF GLUCONATE BASED FORMULATIONS ON OKRA TRIAL 13: Trial was conducted to compare the efficacy of various Potassium Magnesium Sulphate containing products in improving the growth and yield parameters of Okra

The trial was conducted in RBD and it consisted of 6 treatments which were replicated 4 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 13: COMPARISON BETWEEN DIFFERENT POTASSIUM MAGNESIUM SULPHATE CONTAINING PRODUCTS IN IMPROVING THE GROWTH AND YIELD PARAMETERS OF OKRA PLANTS

Treatments Plant height
(cm) % IOC Number of flowers/plant % IOC Number of fruits / plant % IOC Yield (g/plant) % IOC
Control 22.10 - 0.40 - 1.80 - 36.38 -
KMgS (Trio) 26.80 21.27 0.50 25 2.20 22.22 41.53 14.17
KMgS (Destiny) 27.70 25.34 0.50 25 2.40 33.33 41.58 14.28
PGF+MGF (3.0ml/L) 26.90 21.72 0.47 17.5 2.2 22.22 41.50 14.07
PGF+MGF (2.5ml/L) 27.80 25.79 0.40 0 2.10 16.67 37.61 3.39
PGF+MGF (2.0ml/L) 27.30 23.53 0.40 0 2.10 16.67 37.80 3.91
* Data presented in the table is a mean of 4 replicates (Potassium Gluconate Formulation = PGF, MGF = Magnesium Gluconate Formulation).
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control).
EFFECT OF GLUCONATE BASED FORMULATIONS ON BRINJAL TRIAL 14: Trial was conducted to optimize the application rates of Plant Diet in two varieties of Brinjal. The two varieties of Brinjal selected for the study were Sukanya and Uttkarsha.
The trial was conducted in RBD format for each variety and it consisted of 5 treatments which were replicated 4 times. The formulations were sprayed 2 times during the growing season as per treatment requirement. Observations were recorded periodically up to harvest.
TABLE 14: EFFICACY OF DIFFERENT LEVELS OF PLANT DIET ON YIELD PARAMETERS OF BRINJAL

Treatments SUKANYA UTTKARSHA

Fruit number % IOC Yield (Qha-1) % IOC Fruit number % IOC Yield (Qha-1) % IOC
Control 12.2 - 116.63 - 4.25 - 46.42 -
PGF+MGF (2.5 ml/L) 13.2 8.2 126.43 8.4 5.25 23.53 51.11 10.1
PGF+MGF (3 ml/L) 14.4 18.03 137.1 17.82 6.5 52.94 52.11 12.26
PGF+MGF (4 ml/L) 12.8 4.92 125.14 7.3 5.5 29.41 50.69 9.2
PGF+MGF (5
ml/L) 13.4 9.84 127.36 9.2 5.25 23.53 49.55 6.74
* Data presented in the table is a mean of 4 replicates
The plants treated with Gluconate & plant nutrition supplement based formulations were high yielding as compared to the chemical treated (control). The best results of PGF+MGF in both the varieties of Brinjal were obtained when it was applied at the rate of 3 ml/L of water. The application of PGF+MGF was more effective on Sukanya compared to Uttkarsha. Application of PGF+MGF at the rate of 3 ml/L of water can be recommended to the farmers.
In another aspect of the invention, there is provided a process for the production of plant nutrition supplement formulation. Said process comprises following steps:
a) reaction of a calcium gluconate with an acid to produce gluconic acid;
b) reaction of gluconic acid produced in step (a) with metal oxide to produce corresponding metal gluconate;
c) optionally, reacting calcium gluconate with boric acid to produce calcium boro-gluconate;
d) reaction of metal gluconate(s) produced in step (b) or (c) or combination thereof with plant nutrient supplement to produce said formulation along with stirring the solution for approximately 1 hour at approximately 60 rpm.

The metal gluconates comprises magnesium gluconate, ferrous gluconate, boro gluconate, manganese gluconate, potassium gluconate, zinc gluconate or combination thereof while the metal oxide is selected from a group comprising of magnesium, ferrous, manganese, potassium carbonate, zinc or combination thereof. The plant nutrition supplement comprises amino acid, sea weed, humic, fulvic, ascorbic acid or combinations thereof.
The invention is now illustrated by various examples and accompanying drawings, which are not meant to limit the scope of the invention in any manner. All embodiments that may be obvious to a skilled person would fall within the scope of the present invention
EXAMPLE 1: PRODUCTION OF FERROUS GLUCONATE & PLANT NUTRITION SUPPLEMENTS FORMULATION:
1. Calcium Gluconate (M) +Sulfuric Acid (M) Gluconic Acid (2M)
2. Gluconic Acid (2M) + Ferrous 0xide (M) Ferrous Gluconate (M)
3. Ferrous Gluconate (24%) + Amino (1%) + Seaweed Final Formulation (1%) + Humic (1%) + Fulvic (1%) + Ascorbic Acid (1%) (FeGF)
Ferrous gluconate was mixed with various ingredients of plant nutrition supplements (amino acid, seaweed, humic, fulvic & ascorbic acid) and stirred for 1 hour at 60 RPM. The final formulations (FeGF) are highly stable with longer shelf life of more than 3 years.
EXAMPLE 2: PRODUCTION OF CALCIUM BORO GLUCONATE & PLANT NUTRITION SUPPLEMENTS FORMULATION
1. Calcium Gluconate (24%) + Boric Acid (4.8%) Calcium Borogluconate
2. Calcium Borogluconate (24%) + Amino (1%) + Seaweed Final 1%) + Humic (1%) + Fulvic (1%) + Ascorbic Acid (1%) Formulation
(CBGF)

Calcium gluconate was mixed with boric acid and various ingredients of plant nutrition supplements (amino acid, seaweed, humic, fulvic & ascorbic acid) and stirred for 1 hour at 60 RPM. The final formulations (CBGF) are highly stable with longer shelf life of more than 3 years.
EXAMPLE 3: PRODUCTION OF POTASSIUM GLUCONATE & PLANT NUTRITION SUPPLEMENTS FORMULATION
1. Calcium Gluconate (M) + Sulfuric Acid (M) Gluconic Acid (2M)
2. Gluconic Acid (2M) +Potassium Carbonate (M) Potassium Gluconate
(M)
3. Potassium Gluconate (24%) + Amino (1%) + Seaweed Final
(1%) + Humic (1%) + Fulvic (1%) + Ascorbic Acid (1%) Formulation
(PGF) Potassium gluconate was mixed with various ingredients of plant nutrition supplements (amino acid, seaweed, humic, fulvic & ascorbic acid) and stirred for 1 hour at 60 RPM. The final formulations (PGF) are highly stable with longer shelf life of more than 3 years.
EXAMPLE 4: PRODUCTION OF MAGNESIUM GLUCONATE, POTASSIUM GLUCONATE & PLANT NUTRITION SUPPLEMENTS FORMULATION
1. Calcium Gluconate (M) + Sulfuric Acid (M) Gluconic Acid (2M)
2. Gluconic Acid (2M) + Potassium Carbonate (M) Potassium Gluconate
(M)
3. Gluconic Acid (2M) + Magnesium Oxide (M) Magnesium Gluconate
(M)
4. Potassium Gluconate (%) + Magnesium Gluconate (%) Final
+ Amino (1%) + Seaweed (1%) + Humic (1%) Formulation
+Fulvic (1%) + Ascorbic Acid (1%) (MGF+PGF)
Potassium Gluconate and Magnesium Gluconate was mixed with various

ascorbic acid) and stirred for 1 hour at 60 RPM. The final formulations (MGF+PGF) are highly stable with longer shelf life of more than 3 years. The formulation is further suitable for foliar, drip and soil application.
EXAMPLE 5: PROCESS FOR THE PRODUCTION OF PLANT NUTRIENT SYNERGISTIC FORMULATION
Calcium gluconate and sulphuric acid is mixed to produce gluconic Acid. The gluconic acid is further reacted with the potassium carbonate to yield potassium gluconate. Parallely, gluconic acid is also reacted with Magnesium Oxide to produce Magnesium Gluconate. To make up the final formulation potassium gluconate is mixed with magnesium gluconate along with plant nutrient supplements like sea weed , humic, amino acid, fulvic, ascorbic acid etc. along with stirring for 45 minutes at 65 rpm.
The present invention will be explained further with reference to non-limiting embodiments of the invention.
In an embodiment of the invention, there is provided a process for the production of other derived metal gluconate from calcium gluconate.
In another embodiment of the invention, there is provided a synergistic plant nutritional formulation comprising mixture of metal gluconates along with nutrient supplements.
In another embodiment of the invention there is provided a synergistic plant nutritional formulation comprising calcium boro-gluconate.
In another embodiment of the invention there is provided a process for preparation of calcium boro-gluconate from calcium gluconate wherein said process comprises reaction of calcium gluconate with boric acid for the production of calcium boro-gluconate.

WE CLAIM
1. A plant nutrition supplement formulation comprising metal gluconate(s) and nutrition supplement(s) selected from a group comprising of amino acid, sea weed, humic, fulvic, ascorbic acid or salt or derivative thereof or combinations thereof.
2. The plant nutrition supplement formulation as claimed in claim 1, wherein said metal gluconate is present in an amount ranging from 18-36% and preferably around 24%.
3. The plant nutrition supplement formulation as claimed in claim 1, wherein said plant nutrition supplement is present in an amount ranging from 0.5 to 5% and preferably around 1%.
4. The plant nutrition supplement formulation as claimed in claim 1, wherein said metal gluconate is selected from a group comprising of magnesium gluconate, ferrous gluconate, boro gluconate, manganese gluconate, potassium gluconate, zinc gluconate or combination thereof.
5. The plant nutrition supplement formulation as claimed in claim 1, wherein said formulation is stable for a longer life of more than 3 years.
6. The plant nutrition supplement formulation as claimed in claim 1, wherein said formulation enhances the yield of the treated plant as compared to the chemical treated plants.
7. A process for the production of plant nutrition supplement formulation comprising:

a) reaction of a calcium gluconate with an acid to produce gluconic acid;
b) reaction of gluconic acid produced in step (a) with metal oxide to produce corresponding metal gluconate;
c) optional, reaction of calcium gluconate with boric acid to produce calcium borogluconate;
d) reaction of metal gluconate(s) produced in step (b) or (c) or combination thereof with plant nutrient supplement(s) along with

stirring the solution for approximately 1 hour at approximately 60 rpm to produce said formulation.
8. The process as claimed in claim 7, wherein said metal gluconate is selected from a group comprising of magnesium gluconate, ferrous gluconate, boro gluconate, manganese gluconate, potassium gluconate, zinc gluconate or combination thereof.
9. The process as claimed in claim 7, wherein said metal gluconate is present in an amount ranging from 18%-36% and preferably around 24%.
10. The process as claimed in claim 7, wherein said plant nutrition supplement is present in an amount ranging from 0.5% to 5% and preferably around 1%.
11. The process as claimed in claim 7, wherein said metal oxide is selected from a group comprising of magnesium, ferrous, manganese, potassium carbonate, zinc or combination thereof.
12. The process as claimed in claim 7, wherein said acid is a strong acid, preferably sulphuric acid.
13. The process as claimed in claim 7, wherein said plant nutrition supplement is selected from a group comprising of amino acid, sea weed, humic acid, fulvic acid, ascorbic acid or salt or derivative thereof or combinations thereof.
14. The process as claimed in claim 7, wherein said formulation is stable for a longer life of more than 3 years.
15. The process as claimed in claim 7, wherein said formulation increases yield of the treated plant as compared to the chemical treated plants.