1. A system (200) for preserving a biological specimen, the system (200) comprising:
a biological chamber (130) configured to store the biological specimen;
a perfusion fluid reservoir (110) fluidly connected to the biological chamber (130),
the perfusion fluid reservoir (110) is configured to store perfusion fluid,
wherein, the biological chamber (130) is positioned downstream of the reservoir (110) and
the perfusion fluid is selectively supplied to the biological chamber (130) to perform
perfusion of the biological specimen; and
a drain plug (420) provisioned at an outlet of the biological chamber (130), wherein
the drain plug (420) allows at least a portion of the perfusion fluid to drain out of the
biological chamber (130).
2. The system (200) as claimed in claim 1 comprises a buffer chamber (210) fluidly
connected to the drain plug (420), wherein the buffer chamber (210) is configured to
collect the perfusion fluid draining out of the biological chamber (130).
3. The system (200) as claimed in claim 1 comprises a waste fluid reservoir (230) fluidly
connected to the buffer chamber (210), wherein the waste fluid reservoir (230) selectively
receives and stores the drained-out perfusion fluid from the buffer chamber (210).
4. The system (200) as claimed in claim 3, wherein the waste fluid reservoir (230) selectively
receives the drained-out perfusion fluid from the buffer chamber (210) based on conditions
including pre-defined intervals and level of drained-out perfusion fluid in the buffer
chamber (210) not being more within a threshold range.
5. The system (200) as claimed in claim 1, wherein the biological chamber (130) comprises
a plurality of temperature sensors (340) to monitor temperature of the biological specimen
in the biological chamber (130).6. The system (200) as claimed in claim 1 comprises a pressure sensor (320, 335) fluidly
coupled to the perfusion fluid reservoir (110), wherein the pressure sensor (320) is used to
monitor and control pressure of the perfusion fluid supplied to the biological chamber
(130).7. The system (200) as claimed in claim 1 comprises a stabilizer (355) coupled to the
biological chamber (130), wherein the stabilizer (355) is configured to support and
maintain orientation of the biological chamber (130).
8. The system (200) as claimed in claim 7 comprises an orientation sensor (350) associated
with the stabilizer (355), wherein the orientation sensor (350) is configured to monitor and
control the orientation of the biological chamber (130).
9. The system (200) as claimed in claim 1 comprises a pH sensor (370) fluidly connected to
the buffer chamber (210), wherein the pH sensor (370) is configured to measure pH of the
perfusion fluid drained out of the biological chamber (130).
10. The system (200) as claimed in claim 1 comprises a cleaning reservoir (390) configured to store a cleaning fluid to clean the pH sensor (370).
11. The system (200) as claimed in claim 1, wherein the perfusion fluid is supplied from the
perfusion fluid reservoir (110) to the biological chamber (130) at pre-defined time
intervals, duration, and at pre-defined flow rate.
12. The system (200) as claimed in claims 1, 4, 9 and 10 comprises a three-way valve (380)
fluidly connected to the buffer chamber (210), wherein the three-way valve (380) allows
flow of cleaning fluid from the cleaning reservoir (390) to clean the pH sensor (370).
13. The system (200) as claimed in claim 1 comprises a first pump (120) fluidly coupled to
the perfusion fluid reservoir (110), wherein the first pump (120) is configured to supply
the perfusion fluid from the perfusion fluid reservoir (110) to the biological chamber (130).
14. The system (200) as claimed in claim 3 comprises a second pump (220) fluidly coupled to
buffer chamber (210), wherein the second pump (220) is configured to drain out the
perfusion fluid from the biological specimen, collected in the buffer chamber (210) to the
waste fluid reservoir (230.
15. The system (200) as claimed in claims 1 and 9, wherein pH of the biological specimen is
at least one of maintained or altered, based on feedback from the pH sensor (370) by at
least one of varying the flowrate of the perfusion fluid to be supplied from the perfusion fluid reservoir (110) to the biological chamber (130), time interval of perfusion, and
changing composition of the perfusion fluid.
16. The system (200) as claimed in claims 1 and 5, wherein the perfusion fluid reservoir (110)
and the biological chamber (130) are maintained at pre-defined temperature level based
on feedback from the plurality of temperature sensors (340).
17. The system as claimed in claim 1 comprises a control unit associated with the plurality of
temperature sensors (340), the pH sensor (370) and the orientation sensor (350) configured
to:
receive and communicate data sensed by the plurality of temperature sensors (340)
the pH sensor (370) and the orientation sensor (350) to a remote device; and
selectively vary parameters including temperature, pressure and pH of the
biological specimen, perfusion fluid flow rate and orientation of the biological chamber
(130) based on the data sensed by respective sensors (340, 320 370 and 350).