The present disclosure relates generally to wound healing compositions. More specifically, the disclosure provides a wound hydrating composition for topical application comprising amino acids, Curcuma longa, and glycerine. The disclosure also provides a method of preparing the composition.
BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art. [0003] Wound healing process involves expression of various proteins and amino acids along with anti-inflammatory molecules. However, the rigorous processes expressed during deep wound healing results into wound area dryness due to cell apoptosis. This results into slow healing and post healing marks. On the other hand, it is also not recommended to keep the wound moisturized as this also results in slow healing. Therefore, it becomes imperative to supply the wound area with sufficient moisture to fasten wound healing in a proper way by providing cell with supplements, by not letting the wound dry quickly as well as not keeping the wound moisturized for longer time.
[0004] The inventors of the present disclosure overcome such a need for a composition that can balance the moisturization on the wounds to promote healing.
OBJECTS OF THE INVENTION
[0005] An object of the present disclosure is to provide a wound hydrating composition for topical application that supplies nutrients to the wound, hydrates the wound and promotes wound healing.
[0006] An object of the present disclosure is to provide a wound hydrating composition that maintains the balance of not letting the wound dry up and not over hydrating both of which may slow healing.

[0007] Another object of the present disclosure is to provide a method of preparation of the wound hydrating composition.
SUMMARY OF THE INVENTION
[0008] This summary is provided to introduce a selection of concepts in a
simplified form that are further described below in Detailed Description section.
This summary is not intended to identify key features or essential features of the
claimed subject matter, nor is it intended to be used as an aid in determining the
scope of the claimed subject matter.
[0009] Aspects of the present disclosure provide a composition suitable for wound
hydration and healing.
[0010] In an aspect, the present disclosure provides a wound hydrating
composition for topical application comprising active components of amino acids
and Curcuma longa.
[0011] In an embodiment, the composition comprises amino acids obtained from
egg yolk, bovine serum albumin or combination thereof, extracts of Curcuma
longa, glycerine, and 2-amino-2-methylpropanol.
[0012] In an embodiment, the extract of Curcuma longa may be obtained from
rhizomes of Curcuma longa.
[0013] In an embodiment, the composition comprises amino acids obtained from
egg yolk, bovine serum albumin or combination thereof in a range of about
70%w/v to about 80%w/v of the composition, extracts of Curcuma longa in a
range of about 5%w/v to about 10%w/v of the composition, glycerine in a range
of about 7% v/v to about 10% v/v of the composition, and 2-amino-2-
methylpropanol in less than about 2%v/v of the composition.
[0014] In an embodiment, the composition comprises amino acids obtained from
egg yolk, bovine serum albumin or combination thereof in about 70%w/v of the
composition, extracts of Curcuma longa in about 5%w/v of the composition,
glycerine in about 7% v/v of the composition, and 2-amino-2-methylpropanol in
less than 2% v/v of the composition.

[0015] In an aspect, the present disclosure provides a method of preparation of a wound hydrating formulation for topical application, the method comprising the steps of: (a) mixing amino acids obtained from egg yolk, bovine serum albumin or combination thereof in a solvent and preparing an emulsion; (b) adding extracts of Curcuma longa to the emulsion; (c) mixing glycerine dropwise and homogenizing the emulsion; and (d) adding drops of 2-amino-2-methylpropanol and one or more additive(s) followed by lyophilizing to give the formulation. [0016] In another aspect, the present disclosure provides a method of treatment, prophylaxis or amelioration of wounds in a subject by administering an effective amount of the wound hydrating composition.
[0017] Other aspects of the invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learnt by the practice of the invention.
DETAILED DESCRIPTION OF THE INVENTION
[0018] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.
[0019] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply. [0020] Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in

various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0021] In some embodiments, numbers have been used for quantifying weights, percentages, ratios, and so forth, to describe and claim certain embodiments of the invention and are to be understood as being modified in some instances by the term "about." Accordingly, in some embodiments, the numerical parameters set forth in the written description and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable. The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements. [0022] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0023] As used in the description herein and throughout the claims that follow, the meaning of "a," "an," and "the" includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of "in" includes "in" and "on" unless the context clearly dictates otherwise. [0024] Unless the context requires otherwise, throughout the specification which follow, the word "comprise" and variations thereof, such as, "comprises" and "comprising" are to be construed in an open, inclusive sense that is as "including, but not limited to."

[0025] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein. [0026] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. "such as") provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention. [0027] Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limitations. Each group member can be referred to and claimed individually or in any combination with other members of the group or other elements found herein. One or more members of a group can be included in, or deleted from, a group for reasons of convenience and/or patentability. When any such inclusion or deletion occurs, the specification is herein deemed to contain the group as modified.
[0028] The description that follows, and the embodiments described therein, is provided by way of illustration of an example, or examples, of particular embodiments of the principles and aspects of the present disclosure. These examples are provided for the purposes of explanation, and not of limitation, of those principles and of the disclosure.
[0029] It should also be appreciated that the present disclosure can be implemented in numerous ways, including as a system, a method or a device. In this specification, these implementations, or any other form that the invention may take, may be referred to as processes. In general, the order of the steps of the disclosed processes may be altered within the scope of the invention. [0030] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.

[0031] The following discussion provides many example embodiments of the
inventive subject matter. Although each embodiment represents a single
combination of inventive elements, the inventive subject matter is considered to
include all possible combinations of the disclosed elements. Thus if one
embodiment comprises elements A, B, and C, and a second embodiment
comprises elements B and D, then the inventive subject matter is also considered
to include other remaining combinations of A, B, C, or D, even if not explicitly
disclosed.
[0032] As described herein, the term 'effective amount' refers to the amount of
the composition or formulation required to bring about a change or improvement
in a subject without side effects or overdosing.
[0033] The term, "subject" as used herein refers to an animal, preferably a
mammal, and most preferably a human. The term "mammal" used herein refers to
warm-blooded vertebrate animals of the class 'mammalia' , including humans,
characterized by a covering of hair on the skin and, in the female, milk-producing
mammary glands for nourishing the young, the term mammal includes animals
such as cat, dog, rabbit, bear, fox, wolf, monkey, deer, mouse, pig and human.
[0034] The term, 'management', or 'treatment' as used herein refers to alleviate,
slow the progression, attenuation, prophylaxis or as such treat the existing
condition or enhancement of recovery. Treatment also includes treating,
preventing development of, or alleviating to some extent, one or more of the
symptoms of the condition.
[0035] In an embodiment, the present disclosure provides a wound hydrating
composition for topical application comprising active components of amino acids
and Curcuma longa.
[0036] In an embodiment, the present disclosure provides a composition that
supplements essential nutrients to the wound. Primarily, the composition provides
amino acids that can rebuild the protein structures in the wounds.
[0037] Apart from being the active ingredient, Curcuma longa can also double up
as a preservative. In an embodiment, the composition may comprise whole, an
extract or a part of Curcuma longa.

[0038] In an embodiment, the extract or part may be obtained from the group
comprising of root, leaves, shoot, fruits, rhizome, seed, stem, barks, flower, sap,
bud or combinations thereof of Curcuma longa.
[0039] In an embodiment, the composition comprises amino acids obtained from
egg yolk, bovine serum albumin or combination thereof, extracts of Curcuma
longa, glycerine, and 2-amino-2-methylpropanol.
[0040] Glycerine works as a humectant, emollient or binder; while 2-amino-2-
methylpropanol functions as a buffering agent and surfactant.
[0041] In an embodiment, the composition comprises amino acids obtained from
egg yolk, bovine serum albumin or combination thereof in a range of about
70%w/v to about 80%w/v of the composition, extracts of Curcuma longa in a
range of about 5%w/v to about 10%w/v of the composition, glycerine in a range
of about 7% v/v to about 10% v/v of the composition, and 2-amino-2-
methylpropanol in an amount of less than 2% v/v of the composition.
[0042] In a preferred embodiment, the extract of Curcuma longa may be obtained
from the rhizomes of Curcuma longa. The extract may be obtained by any process
or technique well-known in the art. In an embodiment, the extract may be an
aqueous or alcoholic extract. In a preferred embodiment, the extract may be a dry
extract from rhizomes of Curcuma longa or turmeric powder.
[0043] In an embodiment, the composition comprises amino acids obtained from
egg yolk, bovine serum albumin or combination thereof in about 70%w/v of the
composition, extract of rhizome of Curcuma longa in about 5%w/v of the
composition, glycerine in about 7% v/v of the composition, and 2-amino-2-
methylpropanol in less than 2%v/v of the composition.
[0044] The composition of the present disclosure fastens the process of wound
healing by providing adequate moisture and hydration to the skin such that the
moisturization is balanced between being very high that can increase bacterial
growth and decrease wound healing, or being very low to cause dryness.
[0045] The composition is amino-acid-based such that the wound healing process
is fastened by providing cell with amino acid supplements obtained from natural
sources.

[0046] In an embodiment, the present disclosure provides a wound hydrating
formulation for topical application comprising amino acids obtained from egg
yolk, bovine serum albumin or combination thereof, extracts of Curcuma longa,
glycerine, 2-amino-2-methylpropanol, and one or more additive(s).
[0047] In an embodiment, the additive may be selected from a solvent,
humectants, fragrance, coloring agent, preservatives, antimicrobials, or
combinations thereof. However, a person of skill in the art would understand that
any other additive(s) may be employed without going beyond the spirit and scope
of the present disclosure.
[0048] In an embodiment, the additive may be present in a range of about 0.2%
w/v to about 0.5% w/v of the formulation.
[0049] In an embodiment, the solvent may be selected from water, oil, or
combinations thereof. However, a person of skill in the art would understand that
any other solvent(s) may be employed without going beyond the spirit and scope
of the present disclosure.
[0050] In an embodiment, the humectants may be selected from beeswax, shea
butter, cocoa butter, castor oil, or combinations thereof. However, a person of
skill in the art would understand that any other humectant(s) may be employed
without going beyond the spirit and scope of the present disclosure.
[0051] In an embodiment, the fragrance may be selected from rose, chamomile,
sandalwood, lavender, lemongrass, or combinations thereof. However, a person of
skill in the art would understand that any other fragrance(s) may be employed
without going beyond the spirit and scope of the present disclosure.
[0052] In an embodiment, the coloring agent may be selected from any natural or
organic color including annatto, beta-carotene and turmeric extract or food grade
colors. However, a person of skill in the art would understand that any other
coloring agent(s) may be employed without going beyond the spirit and scope of
the present disclosure.
[0053] In an embodiment, the formulation comprises amino acids obtained from
egg yolk, bovine serum albumin or combination thereof in about 70%w/v of the
formulation, extract of rhizome of Curcuma longa in about 5%w/v of the

formulation, glycerine in about 7% v/v of the formulation, 2-amino-2-
methylpropanol in less than about 2%v/v of the formulation, and fragrance in
about 0.5% v/v of the formulation.
[0054] In an embodiment, the formulation is a solid, semi-solid, or liquid. In an
embodiment, the formulation may be a powder, capsule, paste, ointment, lotion,
emulsion, cream, microparticles, or nanoparticles. In a preferred embodiment, the
formulation is a dry powder.
[0055] In some embodiments, the formulation may be used by mixing the dry
powder with a solvent, preferably water and applying on the wound. The
formulation is topical so may be directly applied on the open wound or may be
applied via a cotton swab or clean cloth.
[0056] In another embodiment, the present disclosure provides a method of
preparation of a wound hydrating formulation for topical application, the method
comprising the steps of: (a) mixing amino acids obtained from egg yolk, bovine
serum albumin or combination thereof in a solvent and preparing an emulsion; (b)
adding extracts of Curcuma longa to the emulsion; (c) mixing glycerine dropwise
and homogenizing the emulsion; and (d) adding drops of 2-amino-2-
methylpropanol and one or more additive(s) followed by lyophilizing to give the
formulation.
[0057] In an embodiment, the emulsion may be prepared using a solvent such as
water, oil, or combinations thereof.
[0058] In another embodiment, the present disclosure provides a method of
treatment, prophylaxis or amelioration of wounds in a subject by applying an
effective amount of the wound hydrating composition or formulation on the
wound.
[0059] In an embodiment, the formulation or composition may be employed with
other anti-microbials or wound healing compositions.
[0060] While the foregoing describes various embodiments of the disclosure,
other and further embodiments of the disclosure may be devised without departing
from the basic scope thereof. The scope of the invention is determined by the
claims that follow. The invention is not limited to the described embodiments,

versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art. EXAMPLES
[0061] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary. [0062] MATERIALS: The chemicals were obtained from Sigma, Merck, HiMedia, or SRL chemicals. Turmeric extract was procured locally in the form of dry powder.
[0063] EXAMPLE 1: Preparation of wound hydrating formulation for topical application
[0064] A dry powder formulation was prepared as per the composition provided in Table 1. The amino acid was incorporated into an emulsion using water as a solvent. Added dry powder of turmeric extract into the emulsion. Dropwise mixed glycerine till homogenous solution was obtained. Added about 4 drops of 2-amino-2-methylpropanol (AMP) along with the rose fragrance, and lyophilized the paste by freezing for 10 minutes then primary drying, freezing again for 35 minutes then secondary drying for 51 minutes, followed by sealing and capping 1.3 minutes to give the formulation as dry powder, lg of the dry powder was mixed with 5 mL water and applied to the wounds.

Table 1: Composition for dry formulation

Ingredients % w/v or
v/v Function
Amino acid from BSA and egg yolk 70 % w/v Active ingredient(oil phase)
Turmeric extracts 5 % w/v Active ingredient, preservative (oil phase)
Glycerin 7 % v/v Humectants, emollient and water binder
2-amino-2-
methylpropanol
(AMP) 4 drops Buffering agent, surfactant
Fragrance 0.5% v/v Rose agent
[0065] From the foregoing, it will be appreciated that, although specific embodiments of the invention have been described herein merely for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention and should not be construed so as to limit the scope of the invention or the appended claims in any way.

We Claim:

1. A wound hydrating composition for topical application comprising amino acids obtained from egg yolk, bovine serum albumin or combination thereof, Curcuma longa, glycerine, and 2-amino-2-methylpropanol.
2. The composition as claimed in claim 1, wherein the composition comprises whole, an extract or a part of Curcuma longa.
3. The composition as claimed in claim 2, wherein the extract or part is obtained from the group comprising of root, leaves, shoot, fruits, rhizome, seed, stem, barks, flower, sap, bud or combinations thereof of Curcuma longa.
4. The composition as claimed in claim 2, wherein the extract is a dry extract
from rhizomes of Curcuma longa or turmeric powder.
5. The composition as claimed in claims 1-4, wherein the composition comprises amino acids obtained from egg yolk, bovine serum albumin or combination thereof in a range of 70%w/v to 80%w/v of the composition, extracts oi Curcuma longa in a range of 5%w/v to 10%w/v of the composition, glycerine in a range of 7% v/v to 10% v/v of the composition, and 2-amino-2-methylpropanol in an amount of less than 2% v/v of the composition.
6. The composition as claimed in claim 5, wherein the composition comprises amino acids obtained from egg yolk, bovine serum albumin or combination thereof in 70%w/v of the composition, extract of rhizome of Curcuma longa in 5%w/v of the composition, glycerine in 7% v/v of the composition, and 2-amino-2-methylpropanol in less than 2%v/v of the composition.
7. A wound hydrating formulation for topical application comprising amino acids obtained from egg yolk, bovine serum albumin or combination thereof, extracts of

Curcuma longa, glycerine, 2-amino-2-methylpropanol, and one or more additive(s).
8. The formulation as claimed in claim 7, wherein the formulation is a powder, capsule, paste, ointment, lotion, emulsion, cream, microparticles, or nanoparticles.
9. The formulation as claimed in claim 7, wherein the formulation is a dry powder.
10. A method of preparation of a wound hydrating formulation for topical
application, the method comprising the steps of: (a) mixing amino acids obtained
from egg yolk, bovine serum albumin or combination thereof in a solvent and
preparing an emulsion; (b) adding extracts of Curcuma longa to the emulsion; (c)
mixing glycerine dropwise and homogenizing the emulsion; and (d) adding drops
of 2-amino-2-methylpropanol and one or more additive(s) followed by
lyophilizing to give the formulation.