The present invention relates to a hand sanitizer composition. Specifically, the present invention relates to an herbal hand sanitizer composition having antimicrobial, antibacterial, antifungal, antiviral, and cleansing properties. The present invention further relates to an alcohol-free herbal hand sanitizer composition comprising natural or herbal ingredients.
BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art. [0003] Hand hygiene is a critical component in limiting or preventing the transmission of many diseases. The objective of this study was to prepare ethanol free hand sanitizer gels based on natural materials that contain some essential oils (EOs) that would be effective against pathogens.
[0004] Hand-sanitizer compositions have become a global phenomenon and have developed into larger market, especially after the surge of COVID-19 pandemic. These cleansing compositions rely on thousands of different synthetic chemicals employed in personal care products purported for use on human skin. Most of these chemicals are actually found to cause adverse health risks but are still being used.
[0005] Nowadays, taking note of the importance of natural ingredients in almost all the products to avoid harmful side-effects, many producers / manufacturers have started using natural ingredients along with the synthetic chemicals. However, all-natural hand-sanitizer composition with adequate consumer performance and a limited number of raw materials is a daunting task. Further, the raw material availability is scarce when compared with the synthetic ingredients.
[0006] Additionally, the drawbacks currently associated with the natural ingredients-based hand-sanitizer compositions include costly, requirement of high

concentrations for antimicrobial property, undesirable colors and odors, poor stability, and are not effective against a broad spectrum of microorganisms. [0007] Further, prior art cleansing compositions do not combine effective moisturizing and cleansing properties using only naturally-derived, naturally-processed, safe, biodegradable ingredients.
[0008] Hence, there is an imminent need for economical and non-toxic eco-friendly hand-sanitizer compositions which are effective against a broad spectrum of mi croorgani sm s.
[0009] The present disclosure overcomes the deficiencies of the art by providing a non-toxic eco-friendly cleansing compositions which are effective against a broad spectrum of microorganisms.
[0010] The prior arts available in this field are generally composed of alcohol with one or two active ingredients and lacking cleansing action. Alcohol itself causes skin irritation and rashes making it a secondary choice. Additionally, the effectiveness of the prior arts has been compromised due to short-term activity. [0011] Thus, there is a great need in the art for pharmaceutical composition that is free of alcohol and comprises natural and bio-safe ingredients for effective and long-lasting action in comparison to prior arts.
[0012] The hand-sanitizer compositions of the present invention provide an effective anti-microbial composition that is active against a host of bacteria, including MRSA. Said composition also provides a soothing effect and is suitable for all skin types. The present invention additionally provides the advantage of being capable of use for skin disinfection.
[0013] The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art.
OBJECTS OF THE INVENTION
[0014] An object of the present invention is to provide a hand sanitizer
composition comprising natural ingredients.
[0015] An object of the present invention is to provide an herbal hand sanitizer
composition comprising natural ingredients.

[0016] An object of the present invention is to provide an alcohol-free herbal
hand sanitizer composition comprising natural ingredients.
[0017] An object of the present invention is to provide an alcohol-free herbal
hand sanitizer composition for hand hygiene.
[0018] An object of the present invention is to provide an alcohol-free herbal
hand sanitizer composition comprising excellent antimicrobial, antibacterial,
antifungal, antiviral, and cleansing activities.
[0019] An object of the present invention is to provide an alcohol-free herbal
hand sanitizer composition that is effective even on Methicillin-Resistant
Staphylococcus Aureus (MRS A).
[0020] An object of the present invention is to provide an alcohol-free herbal
hand sanitizer composition that is effective even on coronaviruses including novel
coronavirus (nCOV-19).
[0021] An object of the present invention is to provide an alcohol-free herbal
hand sanitizer composition for hand hygiene comprising neem oil, eucalyptus oil,
clove oil, Aloe vera gel, Acacia nilotica bark extract, Punica granatum peel extract,
glycerine, Carbopol 940, and pharmaceutical grade water or distilled water.
[0022] Another object of the present invention is to provide a method for
preparing an alcohol-free herbal hand sanitizer gel composition comprising the
steps of:
a. dispersing Acacia nilotica bark extract; Punica granatum peel
extract; glycerine; and aloe vera gel to Carbopol 940 gel in a beaker,
followed by mixing with slow stirring at 25 - 50 deg C temperature;
b. adding neem oil and clove oil dropwise to the dispersion from step
a) with constant stirring to avoid air bubble formation and to obtain
homogenous and uniform gels; and
c. formulation was made 100% v/v using distilled water or
pharmaceutical-grade water.
[0023] Another object of the present invention is to provide a method for preparing an alcohol-free herbal hand sanitizer gel composition comprising the steps of:

a. dispersing Acacia nilotica bark extract (0.1-0.5% v/v); Punica
granatum peel extract (0.1-0.5% v/v); glycerine (3% v/v); and aloe
vera gel (20% v/v) to Carbopol 940 gel in a 250 ml beaker, followed
by mixing with slow stirring at 25 - 50 deg C temperature;
b. adding neem oil and clove oil (each at 0.1% v/v to 1 % v/v) dropwise
to the dispersion from step a) with constant stirring to avoid air
bubble formation and to obtain homogenous and uniform gels; and
c. formulation was made 100% v/v using distilled water or
pharmaceutical-grade water.
[0024] Another object of the present invention is to provide an alcohol-free
herbal hand sanitizer composition that is suitable for all skin types.
[0025] Another object of the present invention is to provide an alcohol-free
herbal hand sanitizer composition that is suitable for cleaning and disinfecting the
hands instantly.
[0026] Another object of the present invention is to provide an alcohol-free
herbal hand sanitizer composition that has a mild and gentle action on the hand skin
of the individual.
[0027] Another object of the present invention is to provide an alcohol-free
herbal hand sanitizer composition that maintains the smoothening and soothing
effect on the skin while providing protection from germs.
[0028] Another object of the present invention is to provide an alcohol-free
herbal hand sanitizer composition for hand hygiene.
[0029] Another object of the present invention is to provide an alcohol-free
herbal hand sanitizer composition for hand hygiene comprising neem oil,
eucalyptus oil, clove oil, Aloe vera gel, Acacia nilotica bark extract, Punica
granatum peel extract, glycerine, Carbopol 940, and pharmaceutical grade water or
distilled water.
[0030] Another object of the present invention is to provide an alcohol-free
herbal hand sanitizer composition that can be used during pre- and post-surgical
hand disinfection applications.

[0031] Yet another object of the present invention is to provide an alcohol-free herbal hand sanitizer composition that can be used in home as well as hospital settings.
[0032] Yet another object of the present invention is to provide an alcohol-free herbal hand sanitizer composition that can be used as a rubbing composition. [0033] Yet another object of the present invention is to provide an alcohol-free herbal hand sanitizer composition that is biodegradable and environment-friendly. [0034] Yet another object of the present invention is to provide an alcohol-free herbal hand sanitizer composition that is helpful in increasing the moisture and oil content of the skin immediately after its application from around 0 hours and to around 8 hours with a negligible change.
[0035] Yet another object of the present invention is to provide an alcohol-free herbal hand sanitizer composition that is Non-irritant/dermatologically safe and dermatologically tested: safe for human skin for long-term use.
SUMMARY OF THE INVENTION
[0036] This summary is provided to introduce a selection of concepts in a
simplified form that is further described below in the detailed description section.
This summary is not intended to identify key features or essential features of the
claimed subject matter, nor is it intended to be used as an aid in determining the
scope of the claimed subject matter.
[0037] The present invention relates to a hand sanitizer composition.
Specifically, the present invention relates to an alcohol-free herbal hand sanitizer
composition having antimicrobial, antibacterial, antifungal, antiviral, and cleansing
properties.
[0038] In an aspect, the present invention relates to a hand sanitizer composition
comprising natural ingredients.
[0039] In an aspect, the present invention relates to an herbal hand sanitizer
composition comprising natural ingredients.
[0040] In an aspect, the present invention relates to an alcohol-free herbal hand
sanitizer composition comprising natural ingredients.

[0041] In an aspect, the present invention relates to an alcohol-free herbal hand
sanitizer composition for hand hygiene.
[0042] In an aspect, the present invention relates to an alcohol-free herbal hand
sanitizer composition for hand hygiene comprising neem oil, eucalyptus oil, clove
oil, Aloe vera gel, Acacia nilotica bark extract, Punica granatum peel extract,
glycerine, Carbopol 940, and pharmaceutical grade water or distilled water.
[0043] In an aspect, the present invention relates to an alcohol-free herbal hand
sanitizer composition comprising excellent antimicrobial, antibacterial, antifungal,
antiviral, and cleansing activities.
[0044] In an aspect, the present invention relates to an alcohol-free herbal hand
sanitizer composition that is effective even on Methicillin-Resistant Staphylococcus
aureus (MRSA).
[0045] In an aspect, the present invention relates to an alcohol-free herbal hand
sanitizer composition that is effective even on coronaviruses including novel
coronavirus (nCOV-19).
[0046] In another aspect, the present invention relates to a method for preparing an
alcohol-free herbal hand sanitizer gel composition comprising the steps of:
a. dispersing Acacia nilotica bark extract; Punica granatum peel
extract; glycerine; and aloe vera gel to Carbopol 940 gel in a beaker,
followed by mixing with slow stirring at 25 - 50 deg C temperature;
b. adding neem oil and clove oil dropwise to the dispersion from step
a) with constant stirring to avoid air bubble formation and to obtain
homogenous and uniform gels; and
c. formulation was made 100% v/v using distilled water or
pharmaceutical-grade water.
[0047] In another aspect, the present invention relates to a method for preparing an
alcohol-free herbal hand sanitizer gel composition comprising the steps of:
a. dispersing Acacia nilotica bark extract (0.1-0.5% v/v); Punica granatum peel extract (0.1-0.5%) v/v); glycerine (3%> v/v); and aloe vera gel (20%> v/v) to Carbopol 940 gel in a 250 ml beaker, followed by mixing with slow stirring at 25 - 50 deg C temperature;

b. adding neem oil and clove oil (each at 0.1% v/v to 1 % v/v) dropwise
to the dispersion from step a) with constant stirring to avoid air
bubble formation and to obtain homogenous and uniform gels; and
c. formulation was made 100% v/v using distilled water or
pharmaceutical-grade water.
[0048] In another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that is suitable for all skin types.
[0049] In another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that is suitable for cleaning and disinfecting the hands
instantly.
[0050] In another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that has a mild and gentle action on the hand skin of the
individual.
[0051] In another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that maintains the smoothening and soothing effect on
the skin while providing protection from germs.
[0052] In another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that can be used during pre- and post-surgical hand
disinfection applications.
[0053] In yet another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that can be used in home as well as hospital settings.
[0054] In yet another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that can be used as rubbing composition.
[0055] In yet another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that is biodegradable and environment-friendly.
[0056] In yet another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that is helpful in increasing the moisture and oil content
of the skin immediately after its application from around 0 hours and to around 8
hours with a negligible change.

[0057] In yet another aspect, the present invention relates to an alcohol-free herbal
hand sanitizer composition that is Non-irritant/dermatologically safe and dermatologically tested: safe for human skin for long-term use. [0058] Various objects, features, aspects, and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments
DETAILED DESCRIPTION OF THE INVENTION
[0059] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure.
[0060] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0061] Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0062] In some embodiments, numbers have been used for quantifying weight percentages, ratios, and so forth, to describe and claim certain embodiments of the invention and are to be understood as being modified in some instances by the term

"about." Accordingly, in some embodiments, the numerical parameters set forth in the written description and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable. The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.
[0063] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0064] As used in the description herein and throughout the claims that follow, the meaning of "a," "an," and "the" includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of "in" includes "in" and "on" unless the context clearly dictates otherwise. [0065] Unless the context requires otherwise, throughout the specification which follow, the word "comprise" and variations thereof, such as, "comprises" and "comprising" are to be construed in an open, inclusive sense that is as "including, but not limited to."
[0066] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein. [0067] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. "such as") provided with respect to certain embodiments herein is intended merely to better illuminate the

invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention. [0068] Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limitations. Each group member can be referred to and claimed individually or in any combination with other members of the group or other elements found herein. One or more members of a group can be included in, or deleted from, a group for reasons of convenience and/or patentability. When any such inclusion or deletion occurs, the specification is herein deemed to contain the group as modified.
[0069] The description that follows, and the embodiments described therein, is provided by way of illustration of an example, or examples, of particular embodiments of the principles and aspects of the present disclosure. These examples are provided for the purposes of explanation, and not of limitation, of those principles and of the disclosure.
[0070] It should also be appreciated that the present disclosure can be implemented in numerous ways, including as a system, a method or a device. In this specification, these implementations, or any other form that the invention may take, may be referred to as processes. In general, the order of the steps of the disclosed processes may be altered within the scope of the invention. [0071] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments. [0072] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements a, b, and c, and a second embodiment comprises elements b and d, then the inventive subject matter is also considered to include other remaining combinations of a, b, c, or d, even if not explicitly disclosed.

[0073] While a particular form of the invention has been illustrated and
described, it will be apparent that various modifications can be made without
departing from the spirit and scope of the invention.
[0074] The present invention relates to a hand sanitizer composition.
Specifically, the present invention relates to an alcohol-free herbal hand sanitizer
composition having antimicrobial, antibacterial, antifungal, antiviral, and cleansing
properties.
[0075] In some embodiments, the present invention provides an alcohol-free
herbal hand sanitizer composition that is biodegradable and environment-friendly.
[0076] In some embodiments, the present invention provides an alcohol-free
herbal hand sanitizer composition that is effective against various microorganisms
including novel coronavirus (nCOV-19) and Methicillin-Resistant Staphylococcus
Aureus (MRSA).
[0077] In some embodiments, the present invention provides an antiseptic and
antimicrobial alcohol-free herbal hand sanitizer composition suitable for all skin types,
preferably is suitable for cleaning the limbs, preferably hands.
[0078] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition comprising natural ingredients.
[0079] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition further comprises a moisturizer.
[0080] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition further comprises a natural gel.
[0081] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition further comprises a natural or safe synthetic
emulsifier/thickener.
[0082] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition further comprises a natural emollient.
[0083] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition further comprises a thickener.
[0084] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition comprising a solvent.

[0085] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition comprising a preservative.
[0086] In some embodiments, the present invention relates to an alcohol-free
herbal hand sanitizer composition wherein the composition further comprises an
aromatic or fragrance generating agent.
[0087] In some embodiments, the present invention provides an alcohol-free
herbal hand sanitizer composition optionally including a pH regulator.
[0088] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a biosurfactant in an amount ranging
from about 0.001%> v/v to about 10.0%> v/v, by weight with respect to the overall
composition.
[0089] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise Chlorhexidine digluconate in an
amount ranging from about 0.01%> v/v to about 5.0%> v/v, preferably in the amount
ranging from about 0.1% v/v to about 3.0%> v/v by weight with respect to the overall
composition.
[0090] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise Tea tree oil in an amount ranging from
about 0.001%) v/v to about 1.0%> v/v, preferably in the amount ranging from about
0.01%) v/v to about 0.3% v/v by weight with respect to the overall composition.
[0091] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a natural gel in an amount ranging from
about 0.001%) v/v to about 1.0%> v/v, preferably in the amount ranging from about
0.01%) v/v to about 0.3% v/v by weight with respect to the overall composition.
[0092] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a moisturizer in an amount ranging
from about 0.01%> v/v to about 5.0% v/v, preferably in the amount ranging from
about 0.01%o v/v to about 3.0% v/v by weight with respect to the overall
composition.
[0093] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a preservative in an amount ranging

from about 0.01%> v/v to about 27.0% v/v by weight with respect to the overall
composition.
[0094] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise an emulsifier in an amount ranging
from about 0.01% v/v to about 10.0 % v/v, preferably in the amount ranging from
about 1.0% v/v to about 8.0% v/v by weight with respect to the overall composition.
[0095] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a thickener in an amount ranging from
about 0.1%) v/v to about 5% v/v, preferably in the amount ranging from about 0.01
% v/v to about 3.0 % v/v by weight with respect to the overall composition.
[0096] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a pH regulator in an amount ranging
from about 0.01% v/v to about 4.0% v/v, preferably in the amount ranging from
about 0.01%) v/v to about 2.0% v/v by weight with respect to the overall
composition.
[0097] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a natural colour in the amount ranging
from about 0.01% v/v to about 1.0% v/v by weight with respect to the overall
composition.
[0098] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a natural fragrance in the amount
ranging from about 0.01% v/v to about 1.0% v/v by weight with respect to the
overall composition.
[0099] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition may further comprise a solvent in a quantity sufficient to
make up the weight percentage to 100% v/v.
[00100] In an embodiment of the present invention, the alcohol-free herbal hand
sanitizer composition does not comprise ethanol, isopropyl alcohol, or any other
alcohol purported to be used in conventional hand-sanitizing compositions.

[00101] In an embodiment of the present invention, the biosurfactant can be selected from glycolipids, Lipopeptides, phospholipids, fatty acids, neutral lipids, polymeric and particulate compounds, and the like.
[00102] In an embodiment of the present invention, the natural gel is aloe vera gel.
[00103] In an embodiment of the present invention, the moisturizer can be selected from jojoba oil, cucumber extract, Coco-glucoside, Shea butter, Cocoa butter, Argan oil, Aloe Vera, Olive oil, Sunflower oil, Avocado oil, Butylene glycol, Vegetable glycerin, and the like.
[00104] In an embodiment of the present invention, the preservative can be selected from sorbic acid, calendula oil, benzoic acid, citric acid, triclosan, triclocarban, phenoxyethanol, benzyl benzoate, benzyl alcohol, zinc pyrithione, benzalkonium chloride, and the like.
[00105] In an embodiment of the present invention, the natural emollient can be selected from aloe vera, shea butter, cocoa butter, argan oil, jojoba oil, cucumber extract, olive oil, sunflower oil, oil of Melaleucae alternifolia (tea tree oil), avocado oil, and the like. Most preferably aloe vera.
[00106] In an embodiment of the present invention, the thickener can be selected from methyl cellulose (HPMC), CMC, guar gum, xanthan gum, carbopol, gum arabic, cornstarch, gelatin, or the combinations thereof.
[00107] In an embodiment of the present invention, the pH regulator can be selected from sodium hydroxide, citric acid, lactic acid, triethanolamine, glacial acetic acid, sodium carbonate, acetic acid, and the like.
[00108] In an embodiment of the present invention, the solvent is purified water, distilled water or pharmaceutical grade water.
[00109] In an embodiment of the present invention, the fragrance is natural fragrance selected from lemon, jasmine, lavender, mint ice, peach, red berry, apple, vanilla, rose, rosemary, mogra, sandalwood, orange, and the like. [00110] In a preferred embodiment of the present invention, the alcohol-free herbal hand sanitizer composition comprises: a) Neem oil;

b) Eucalyptus oil;
c) Clove oil;
d) Aloe vera gel;
e) Acacia nilotica bark extract;
f) Punica granatum peel extract;
g) Glycerine;
h) Carbopol 940; and
i) pharmaceutical grade water or distilled water.
[00111] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises neem oil at a concentration of at least about 0.1%) v/v, more usually at least about 0.2% v/v, at least about 0.3% v/v, at least about 0.4%) v/v, at least about 0.5% v/v, and not more than about 0.6% v/v or about 0.6%) v/v, but usually not more than about 0.7% v/v, or not more than about 1% v/v. Most preferably, the percentage of neem oil is 0.5% v/v by weight with respect to the overall composition.
[00112] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises eucalyptus oil at a concentration of at least about 0.1%) v/v, more usually at least about 0.2% v/v, at least about 0.3% v/v, at least about 0.4% v/v, at least about 0.5% v/v, and not more than about 0.6% v/v or about 0.6%) v/v, but usually not more than about 0.7% v/v, or not more than about 1%> v/v. Most preferably, the percentage of eucalyptus oil is 0.5% v/v by weight with respect to the overall composition.
[00113] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises clove oil at a concentration of at least about 0.1%) v/v, more usually at least about 0.2% v/v, at least about 0.4% v/v, at least about 0.6%) v/v, at least about 0.8% v/v, and not more than about 1.0% v/v or about 2.0%) v/v, but usually not more than about 3.0%> v/v, or not more than about 2% v/v. Most preferably, the percentage of eucalyptus oil is 1% v/v by weight with respect to the overall composition.
[00114] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises Aloe vera gel at a concentration of at least

about 5% v/v, more usually at least about 10% v/v, at least about 15% v/v, at least about 20%) v/v, at least about 25% v/v, and not more than about 30% v/v or about 40%) v/v, but usually not more than about 25% v/v, or not more than about 20% v/v. Most preferably, the percentage of Aloe vera gel is 20% v/v by weight with respect to the overall composition.
[00115] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises glycerine at a concentration of at least about 0.5%) v/v, more usually at least about 0.75% v/v, at least about 1.0% v/v, at least about 1.5% v/v, at least about 2% v/v, at least about 2.5% v/v, at least about 3% v/v, at least about 3.5% v/v, at least about 4% v/v, and not more than about 5.0% v/v or about 10.0% v/v, but usually not more than about 5.0%> v/v, or not more than about 3%> v/v. Most preferably, the percentage of glycerine is 3% v/v by weight with respect to the overall composition.
[00116] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises Carbapol 940 at a concentration of at least about 0.1%) v/v, more usually at least about 0.2% v/v, at least about 0.25% v/v, at least about 0.3% v/v, at least about 0.4% v/v, at least about 0.45% v/v, at least about 0.5%) v/v, at least about 1% v/v, and not more than about 1.0% v/v or about 0.5% v/v, but usually not more than about 0.5% v/v, or not more than about 0.4% v/v. Most preferably, the percentage of Carbapol 940 is 0.25% v/v by weight with respect to the overall composition.
[00117] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises Acacia nilotica bark extract at a
concentration of at least about 0.1% v/v, more usually at least about 0.2% v/v, at
least about 0.4% v/v, at least about 0.6% v/v, at least about 0.8% v/v, and not more
than about 1.0% v/v or about 2.0% v/v, but usually not more than about 3.0%> v/v,
or not more than about 2% v/v. Most preferably, the percentage of Acacia nilotica
bark extract is 0.1 - 0.5% v/v by weight with respect to the overall composition.
[00118] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises Punica granatum peel extract at a concentration of at least about 0.1% v/v, more usually at least about 0.2% v/v, at

least about 0.4% v/v, at least about 0.6%> v/v, at least about 0.8%> v/v, and not more
than about 1.0% v/v or about 2.0% v/v, but usually not more than about 3.0%> v/v,
or not more than about 2% v/v. Most preferably, the percentage of Punica granatum
peel extract is 0.1 - 0.5% v/v by weight with respect to the overall composition.
[00119] In an embodiment of the present invention, the alcohol-free herbal
hand sanitizer composition comprises distilled or pharmaceutical grade water to make 100% v/v composition.
[00120] In another embodiment, the present invention provides a method for
preparing an alcohol-free herbal hand sanitizer gel composition comprising the steps of:
a. dispersing Acacia nilotica bark extract; Punica granatum peel
extract; glycerine; and aloe vera gel to Carbopol 940 gel in a beaker,
followed by mixing with slow stirring at 25 - 50 deg C temperature;
b. adding neem oil and clove oil dropwise to the dispersion from step
a) with constant stirring to avoid air bubble formation and to obtain
homogenous and uniform gels.
c. formulation was made 100% v/v using distilled water or
pharmaceutical-grade water.
[00121] In another embodiment, the present invention provides a method for
preparing an alcohol-free herbal hand sanitizer gel composition comprising the steps of:
a. dispersing Acacia nilotica bark extract (0.1-0.5%) v/v); Punica
granatum peel extract (0.1-0.5%) v/v); glycerine (3% v/v); and aloe
vera gel (20% v/v) to Carbopol 940 gel in a 250 ml beaker, followed
by mixing with slow stirring at 25 - 50 deg C temperature;
b. adding neem oil and clove oil (each at 0.1% v/v to 1 % v/v) dropwise
to the dispersion from step a) with constant stirring to avoid air
bubble formation and to obtain homogenous and uniform gels.
c. formulation was made 100% v/v using distilled water or
pharmaceutical-grade water.

[00122] In an embodiment, the alcohol-free herbal hand sanitizer
composition is provided in the form of a powder, cream, lotion, liquid, or gel. Most
preferably, the alcohol-free herbal hand sanitizer composition realized in
accordance with embodiments of the present disclosure is prepared in gel form.
[00123] In an embodiment, the alcohol-free herbal hand sanitizer
composition of the present invention maybe used on the body part in any manner known to a person skilled in the art.
[00124] In an embodiment, the present invention relates to an alcohol-free
herbal hand sanitizer composition which is useful as an alcohol-free herbal hand sanitizer composition by medical care-givers. The term 'alcohol-free herbal hand sanitizer composition' as used herein throughout the present disclosure denotes the washing compositions that do not require utilization of water or require minimal amount of water (such as use of wet cloth or sponge or wipe) to effect removal of dirt or soil along with the composition. EXAMPLES
[00125] The present disclosure is further explained in the form of following
examples. However, it is to be understood that the foregoing examples are merely illustrative and are not to be taken as limitations upon the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the scope of the invention.
[00126] Example 1: Composition of the alcohol-free herbal hand sanitizer
gel composition Table 1: The composition of all prepared hand sanitizer gels and the control
gel with each ingredient's concentration.

Ingredients Quantity % v/v

Fl F2 F3 F4 F5
Neem oil 0.20 0.30 0.40 0.50 —
Clove oil 0.10 0.20 0.40 1 —

Aloe vera gel 20 20 20 20 20
Acacia nilotica bark extract 0.1 0.20 0.40 0.50
Punica
granatum peel extract 0.1 0.20 0.40 0.50
Glycerine 3 3 3 3 3
Carbopol 940 0.40 0.35 0.30 0.25 0.25
Distilled water q.s tomake 100% v/v q.s to make 100% v/v q.s to make 100% v/v q.s tomake 100% v/v q.s to make 100%
v/v
[00127] Example 2: In-vitro Antimicrobial Activity of the alcohol-free
herbal hand sanitizer gel composition
The antimicrobial effectiveness test demonstrated that the prepared hand sanitizer 5 gels had antimicrobial activities against different gram-positive and gram-negative bacteria {Escherichia coli and Staphylococcus aureus). The antibacterial efficacy was increased as essential oil concentration increased.
Table 2: MIC over different Concentrations

S.No Formulations Concentration MIC (in mm)

E. Coli S. aureus
1. Fl 10% v/v - -

20% v/v - -

50% v/v 10 mm 13 mm

100% v/v 12 mm 12 mm
2 F2 10% v/v - -

20% v/v - -

50% v/v 9 mm 11 mm

100% v/v 13 mm 14 mm
3 F3 10% v/v - -

20% v/v - -

50% v/v 10 mm 12mm

100% v/v 13mm 15 mm
4 F4 10% v/v - -

20% v/v - -

50% v/v 13 mm 16 mm

100% v/v 18 mm 20 mm
5 F5 Formulation without EOs 10% v/v - -

20% v/v - -

50% v/v - -

100% v/v 6 5
[00128] Example 3: Skin irritation testing of the alcohol-free herbal hand
sanitizer gel composition:
The alcohol-free herbal hand sanitizer gel composition from Example 1 is made 5 into 100 ml pockets each and anonymously handed over to 30 volunteers to test the
adverse effects or skin irritancy of the composition. Each of the subjects were
instructed to use 10 ml of formulated gel on an area of 2 square inch to the back of
subjects' hands. The subjects were observed for lesions, redness and irritation.
None of the subjects reported of adverse effects or irritation while using or after D using the composition.
The acceptability study on 30 human volunteers showed that the hand sanitizer gel
did not produce any signs of skin irritation.

Table 3: Volunteer Responses

S.No Response (Feedback) No. of Volunteers (30)
1. Skin irritancy No irritation
2. Excellent 18
3. Good 7
4. Average 5
5. Poor (Below Average) 0
Example 6: Characterization of the alcohol-free herbal hand sanitizer gel
composition:
5 Table 4: Different Observed Parameters

s.
No Parameters
* Fl F2 F3 F4 F5
1. pH 4.8 4.9 5 5 6.5
2. Viscosity 750±40 at
100 rpm to
3565±90
at 20 rpm 720±50 at 100 rpm to 3405±100 at 20 rpm 690±50 at 100 rpm to 3260±100 at 20 rpm 650±50 at 100 rpm to 3200±100 at 20 rpm 640±50 at 100 rpm to 3105±100 at 20 rpm
3. Stability Stable at
different
conditions Stable at
different
conditions Stable at
different
conditions Stable at
different
conditions Stable at
different
conditions
4. Spreadability Easily spreadable Easily spreadable Easily spreadable Easily spreadable Easily spreadable

5. Visual No No No No No
inspection particle particle particle particle particle
observed observed observed observed observed
[00129] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims 5 that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.

We Claim:
1. An herbal hand sanitizer composition, wherein the composition is an
alcohol-free gel composition comprising,
a. neem oil;
b. eucalyptus oil;
c. clove oil;
d. Aloe vera gel;
e. Acacia nilotica bark extract;
f Punica granatum peel extract;
g. glycerine;
h. Carbopol 940; and
i. pharmaceutical grade water or distilled water; characterized in that: - the composition is environment-friendly and biodegradable.
2. The composition as claimed in claim 1, wherein the composition further comprises moisturizer, natural gel, natural emulsifier, natural emollient, thickener, solvent, preservative, fragrance and pH regulator.
3. The composition as claimed in claim 1, wherein the composition is suitable for home and hospital settings.
4. The composition as claimed in claim 3, wherein the composition is a surgical hand-rub composition.
5. The composition as claimed in claim 1, wherein the pH of the composition ranges from about 4.8 to about 6.5.

6. The composition as claimed in claim 1, wherein the viscosity of the composition ranges from about 600 to about 800 at 100 rpm; and from about 3000 to about 3600 at 20 rpm.
5 7. The composition as claimed in claim 1, wherein the composition has
antimicrobial and disinfectant activities.
8. An alcohol-free herbal hand sanitizer gel composition comprising,
a. neem oil in an amount ranging from about 0.1% v/v to about 1% v/v
L0 of the total composition;
b. eucalyptus oil in an amount ranging from about 0.1% v/v to about
1%> v/v of the total composition;
c. clove oil in an amount ranging from about 0.05% v/v to about 2%
v/v of the total composition;
L5 d. Aloe vera gel in an amount ranging from about 5% v/v to about 25%
v/v of the total composition; e. Acacia nilotica bark extract in an amount ranging from about 0.05%
v/v to about 2%> v/v of the total composition;
f Punica granatum peel extract in an amount ranging from about
10 0.05%) v/v to about 2% v/v of the total composition;
g. glycerine in an amount ranging from about 0.5% v/v to about 5%
v/v of the total composition;
h. Carbopol 940 in an amount ranging from about 0.05% v/v to about
2%> v/v of the total composition; and
IS i. pharmaceutical grade water or distilled water to make the
composition 100%) v/v.
9. A method for preparing an alcohol-free herbal hand sanitizer gel
composition comprising the steps of:

a. dispersing Acacia nilotica bark extract; Punica granatum peel
extract; glycerine; and aloe vera gel to Carbopol 940 gel in a beaker,
followed by mixing with slow stirring at 25 - 50 deg C temperature;
b. adding neem oil and clove oil dropwise to the dispersion from step
a) with constant stirring to avoid air bubble formation and to obtain
homogenous and uniform gels.
c. formulation was made 100% v/v using distilled water or
pharmaceutical grade water.
10. A hand sanitizer gel comprising the composition as claimed in claim 1 or 8.