TECHNICAL FIELD
[001] The subject matter described herein in general relates to pharmaceutical and food supplement compositions, and in particular, relates to the field of herbal anti-diabetic compositions.
BACKGROUND OF INVENTION
[002] Diabetes is a chronic metabolic disease characterized by hyperglycemia (increase in blood glucose level). Skeletal muscle is the major insulin-target tissues responsible for maintenance of whole body glucose homeostasis. The impairment of glucose uptake by skeletal muscle cells are regularly seen in type 2 diabetes mellitus (T2DM). An effective inducer of skeletal muscle cell glucose uptake is to be used for the management of diabetes (Taku Nedachi and Makoto Kanzaki (2006) Regulation of glucose transporters by insulin and extracellular glucose in C2C12 myotubes. Am J Physiol Endocrinol Metab. 291: E817-E828). Thus, investigation of the efficacy of organic compounds on skeletal muscle cell glucose uptake for management of T2DM can be important for establishing efficacy of anti-diabetic compositions.
[003] Compositions have been actively researched for treating diabetes. US6498193 discloses a method for treating long-term effects of T2DM using metformin in a dosage 25 - 50% more than the usual dosage. Synthetic drugs such as sulfonyl urea and biguanides suffer from various side-effects such as weight gain and kidney complications. Moreover, the potency of these drugs is such that the patients need to keep a constant check on dietary intake to avoid complications of low blood sugar. In light of such problems herbal medication or supplements have been pursued, as these typically have enhanced bio-efficacy and minimal side-effects. US8993008 reveals a combination of bio-actives that is supposed to be brewed as a tea for treating diabetes. However, there is a need for ensuring medication that remains effective even at low concentrations. A combinatorial approach involving a mix of actives for ensuring high potency at low dosages is much sought after.

SUMMARY OF THE INVENTION
[004] In an aspect of the present invention, there is provided an anti-diabetic
composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini
extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract
weight ratio is in a range of 1:0.5 to 1:3.5.
[005] In another aspect of the present invention, there is provided a process for
preparing the anti-diabetic composition comprising: a) Cinnamomum cassia
extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract
to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, said
process comprising steps of: a) obtaining the Cinnamomum cassia extract; b)
obtaining the Syzygium cumini extract; c) obtaining the at least one additive; and d)
contacting the Cinnamomum cassia extract and the Syzygium cumini extract with
the at least one additive to obtain the composition.
[006] These and other features, aspects, and advantages of the present subject
matter will be better understood with reference to the following description and
appended claims. This summary is provided to introduce a selection of concepts in
a simplified form. This summary is not intended to identify key features or
essential features of the claimed subject matter, nor is it intended to be used to limit
the scope of the claimed subject matter.
BRIEF DESCRIPTION OF THE DRAWINGS
[007] The detailed description is described with reference to the accompanying
figures. The same numbers are used throughout the drawings to reference like
features and components.
[008] Figure 1 illustrates dose response of Cinnamomum cassia (cinnamon) and
Syzygium cumini (jamun) extracts on glucose uptake in skeletal muscle cells, in
accordance with an implementation of the present subject matter.
[009] Figure 2 illustrates effect of different combination of Cinnamomum cassia
(cinnamon) and Syzygium cumini (jamun) extracts on glucose uptake in skeletal
muscle cells, in accordance with an implementation of the present subject matter.
DETAILED DESCRIPTION OF THE INVENTION

[0010] Those skilled in the art will be aware that the present disclosure is subject to
variations and modifications other than those specifically described. It is to be
understood that the present disclosure includes all such variations and
modifications. The disclosure also includes all such steps, features, compositions,
and compounds referred to or indicated in this specification, individually or
collectively, and any and all combinations of any or more of such steps or features.
Definitions
[0011] For convenience, before further description of the present disclosure,
certain terms employed in the specification, and examples are delineated here.
These definitions should be read in the light of the remainder of the disclosure and
understood as by a person of skill in the art. The terms used herein have the
meanings recognized and known to those of skill in the art, however, for
convenience and completeness, particular terms and their meanings are set forth
below.
[0012] The articles "a", "an" and "the" are used to refer to one or to more than one
(i.e., to at least one) of the grammatical object of the article.
[0013] The terms "comprise" and "comprising" are used in the inclusive, open
sense, meaning that additional elements may be included. It is not intended to be
construed as "consists of only".
[0014] Throughout this specification, unless the context requires otherwise the
word "comprise", and variations such as "comprises" and "comprising", will be
understood to imply the inclusion of a stated element or step or group of element or
steps but not the exclusion of any other element or step or group of element or
steps.
[0015] The term "including" is used to mean "including but not limited to".
"Including" and "including but not limited to" are used interchangeably.
[0016] Ratios, concentrations, amounts, and other numerical data may be presented
herein in a range format. It is to be understood that such range format is used
merely for convenience and brevity and should be interpreted flexibly to include
not only the numerical values explicitly recited as the limits of the range, but also
to include all the individual numerical values or sub-ranges encompassed within

that range as if each numerical value and sub-range is explicitly recited. For example, weight ranges of about 0.00125 - 0.005% should be interpreted to include not only the explicitly recited limits of about 0.00125% to about 0.005%), but also to include sub-ranges, such as 0.00125-0.0025%, 0.002-0.005%, and so forth, as well as individual amounts, including fractional amounts, within the specified ranges, for example.
[0017] The term "at least one" is used to mean one or more and thus includes individual components as well as mixtures/combinations.
[0018] For the purposes of the present disclosure, cinnamon extract refers to an extract obtained from Cinnamomum cassia plant that is obtained commercially and is a value-added product.
[0019] For the purposes of the present disclosure, jamun extract refers to an extract obtained from Syzygium cumini plant that is obtained commercially and is a value-added product.
[0020] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference. [0021] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods are clearly within the scope of the disclosure, as described herein. [0022] As mentioned previously, the existing food supplements suffer from drawbacks of toxicity and high cost. Therefore, there is a scope for exploration of synergistic actives combinations which can provide the better efficacy of health promoting benefits such as enhanced cellular glucose-uptake. Herbal or natural combinations could prove effective in providing enhanced glucose-uptake in cells while being non-toxic. The present disclosure aims to solve the said problem by

employing a combination consisting of Cinnamomum cassia extract and Syzygium cumini extract shows the synergistic effect on the cellular glucose-uptake. [0023] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5. In another embodiment of the present disclosure, the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.8 to 1:3.2.
[0024] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:1.
[0025] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3.
[0026] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the Cinnamomum cassia extract comprises polyphenols having weight percentage in a range of 20 - 30 % with respect to the extract. In another embodiment of the present disclosure, the Cinnamomum cassia extract comprises polyphenols having weight percentage in a range of 20 - 29 % with respect to the extract. In yet another embodiment of the present disclosure, the Cinnamomum cassia extract comprises polyphenols having a weight percentage of 20.35 with respect to the extract.
[0027] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, and the Cinnamomum cassia extract comprises polyphenols having weight percentage in a range of 20 - 30 % with respect to the extract.

[0028] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:1 and the Cinnamomum cassia extract comprises polyphenols having a weight percentage in a range of 20 - 30 % with respect to the extract.
[0029] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3, and the Cinnamomum cassia extract comprises polyphenols having weight percentage in a range of 20 - 30 % with respect to the extract.
[0030] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the Syzygium cumini extract comprises flavonoids having a weight percentage in a range of 3 - 10 % with respect to the extract. In another embodiment of the present disclosure, the Syzygium cumini extract comprises flavonoids having a weight percentage in a range of 3.2 - 9.5 % with respect to the extract. The flavonoids are as per references provided such as Muniappan Ayyanar et al., Asian Pacific Journal of Tropical Biomedicine (2012) 240; and Asian J Pharm Clin Res, 9, 2, (2016) 361-363.
[0031] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, and the Syzygium cumini extract comprises flavonoids having a weight percentage in a range of 3 - 10 % with respect to the extract.
[0032] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini

extract weight ratio is 1:1, and the Syzygium cumini extract comprises flavonoids having a weight percentage in a range of 3 - 10 % with respect to the extract. [0033] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3, and the Syzygium cumini extract comprises flavonoids having weight percentage in a range of 3 - 10 % with respect to the extract. [0034] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, the Cinnamomum cassia extract comprises polyphenols having a weight percentage in a range of 20 - 30 % with respect to the extract and the Syzygium cumini extract comprises flavonoids having a weight percentage in a range of 3 - 10 % with respect to the extract. [0035] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the composition comprises: a) Cinnamomum cassia extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition; and b) Syzygium cumini extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition. In another embodiment of the present disclosure, the composition comprises: a) Cinnamomum cassia extract having weight percentage in a range of 0.00125 -0.004%) with respect to the composition; and b) Syzygium cumini extract having a weight percentage in a range of 0.00125 -0.004%> with respect to the composition. [0036] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the composition comprises: a) Cinnamomum cassia extract having concentration in a range of 12.5 -50.0 ug/ml; and b) Syzygium cumini extract having a weight percentage in a range of 12.5 -50.0 ug/ml. In another embodiment of the present disclosure, the composition comprises: a) Cinnamomum cassia extract having concentration in a range of 12.5 -40.0 ug/ml; and b) Syzygium cumini extract having a weight percentage in a range of 12.5-40.0 ug/ml.

[0037] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the composition comprises: a) Cinnamomum cassia extract having concentration in a range of 12.5 -50.0 ug/ml; and b) Syzygium cumini extract having a weight percentage in a range of 12.5 -50.0 ug/m, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5.
[0038] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition; and b) Syzygium cumini extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5. [0039] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition; and b) Syzygium cumini extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:1.
[0040] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition; and b) Syzygium cumini extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3.
[0041] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition; and b) Syzygium cumini extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, the Cinnamomum cassia extract comprises polyphenols having a weight percentage in

a range of 20 - 30 % with respect to the extract, and the Syzygium cumini extract comprises flavonoids having a weight percentage in a range of 3 - 10 % with respect to the extract.
[0042] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the composition further comprises at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler and preservative and combinations thereof. In another embodiment of the present disclosure, the at least one additive is the carrier. In yet another embodiment of the present disclosure, the at least one additive is the chelating agent. In another embodiment of the present disclosure, the carrier is dimethylsulfoxide. Dimethylsulfoxide was used as a carrier in the composition under various experimental conditions. [0043] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler, preservative, and combinations thereof, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5.
[0044] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler, preservative, and combinations thereof, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:1.
[0045] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler, preservative, and combinations thereof, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3.

[0046] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, said process comprising steps of: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; c) obtaining at least one additive; and d) contacting the Cinnamomum cassia extract and the Syzygium cumini extract with the at least one additive to obtain the composition. [0047] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, said process comprising steps of: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; and c) contacting the Cinnamomum cassia extract, and the Syzygium cumini extract to obtain the composition. [0048] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:1, said process comprising steps of: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; c) obtaining the at least one additive; and d) contacting the Cinnamomum cassia extract and the Syzygium cumini extract with the at least one additive to obtain the composition.
[0049] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:1, said process comprising steps of: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; and c) contacting the Cinnamomum cassia extract and the Syzygium cumini extract to obtain the composition.

[0050] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3, said process comprising steps of: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; c) obtaining the at least one additive; and d) contacting the Cinnamomum cassia extract and the Syzygium cumini extract with the at least one additive to obtain the composition.
[0051] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein Cinnamomum cassia extract to Syzygium cumini extract weight ratio is 1:3, said process comprising steps of: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; and c) contacting the Cinnamomum cassia extract and the Syzygium cumini extract to obtain the composition.
[0052] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler and preservative and combinations thereof, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5, said process comprising: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; c) obtaining the at least one additive; and d) contacting the Cinnamomum cassia extract, and the Syzygium cumini extract with the at least one additive to obtain the composition.
[0053] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler and preservative and combinations thereof, wherein Cinnamomum cassia extract to Syzygium cumini extract weight ratio is 1:1, said process comprising: a) obtaining

the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; c) obtaining the at least one additive; and d) contacting the Cinnamomum cassia extract and the Syzygium cumini extract with the at least one additive to obtain the composition.
[0054] In an embodiment of the present disclosure, there is provided a process for preparing the anti-diabetic composition comprising: a) Cinnamomum cassia extract; b) Syzygium cumini extract; and c) at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler and preservative and combinations thereof, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3, said process comprising: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; c) obtaining the at least one additive; and d) contacting the Cinnamomum cassia extract, and the Syzygium cumini extract with the at least one additive to obtain the composition.
[0055] In an embodiment of the present disclosure, there is provided an anti-diabetic composition as described herein, wherein the composition is dispensed in the form of a dietary supplement selected from the group consisting of juices, shakes, vegetable blends, fruit blends, concentrates, and combinations thereof. [0056] In an embodiment of the present disclosure, there is provided an anti-diabetic composition as described herein, wherein the composition is a food supplement.
[0057] In an embodiment of the present disclosure, there is provided an anti-diabetic composition as described herein, wherein the composition is a juice. [0058] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the composition is a concentrate that can be suitably diluted with water or any other well-known edible diluent and consumed as a drink.
[0059] In an embodiment of the present disclosure, there is provided an anti-diabetic composition as described herein, wherein the composition may be taken in prescribed dosages to obtain anti-diabetic health benefits.

[0060] In an embodiment of the present disclosure, there is provided an anti¬diabetic composition as described herein, wherein the composition may be dispensed as part of commercial formulations such as health supplements. EXAMPLES
[0061] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may apply. [0062] As described above, there is a scope for exploration of synergistic actives combinations which can provide the better efficacy of health promoting benefits. The present disclosure reveals an anti-diabetic composition comprising: a) Cinnamomum cassia extract; and b) Syzygium cumini extract, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5. In a surprise finding, it was found that cinnamon {Cinnamomum cassia) and jamun {Syzygium cumini) extract increased the glucose uptake in skeletal muscle cells synergistically. A unique combination of cinnamon and jamun extracts was found to show increased glucose uptake in the skeletal muscle cells that can be used for the management of type-2 diabetes mellitus (T2DM).
Example 1- Materials and methods
[0063] Preparation of Cinnamomum cassia or cinnamon extract: Cinnamon bark extract was sourced from Sami Labs, Bangalore. A 50 mg/ml stock solution was prepared by dissolving 50 mg/mL of cinnamon bark extract in solvent, preferably dimethylsulfoxide (DMSO; 100 %). 12.5 - 50.0 ug/mL of cinnamon bark extract

was prepared by dissolving 0.25 - 1.0 ul in lmL of the dulbecco modified eagle medium (DMEM; Gibco, NY, USA).
[0064] Preparation of Syzygium cumini or jamun extract: Stock solution of jamun extract was prepared by using commercially available extract from Chemloids Life Sciences PVT. LTD., Vijayawada. A stock solution jamun extract powder was prepared by dissolving 50 mg/ml of solvent, preferably dimethyl sulfoxide (DMSO; 100 %). A 12.5 - 50.0 ug/mL of jamun extract was prepared by dissolving 0.25 -1.0 ul in lmL of the Dulbecco's Modified Eagle Medium (DMEM; Gibco, NY, USA).
[0065] Cell cultures and Culture media- The cell culture media for C2C12 were prepared by adding 445 ml DMEM (Dulbecco's modified Eagle's medium) obtained from Gibco, N.Y. with 50 ml of Fetal Bovine Serum (FBS) from Invitrogen, NZ and 5ml of 100 U/ml penicillin / 0.1 mg/ml streptomycin (Sigma USA) and filtered with 0.2 u filter unit. C2C12 murine skeletal muscle precursor cells (myoblasts) were obtained from American Type culture collection (Manassas, VA.) and maintained in proliferation media, consisting of DMEM supplemented with 10% FBS. Myoblasts were induced to differentiate into skeletal muscle myotubes by culturing for 6 days in differentiation media, consisting of DMEM supplemented with 2% horse serum (HS). Differentiation media was replenished every 48 hours.
[0066] Glucose uptake assay- The C2C12 cells were allowed to get 70% confluent and differentiation was initiated. The C2C12 cells were treated with DMEM supplemented with 2 % (v/v) HS. The cells were subsequently re-fed every 48 h with DMEM supplemented with 2% HS and 1% penicillin/streptomycin. At the 6th days of differentiation, the culture media were removed and replaced with serum free DMEM containing no glucose and incubated at 37 °C with 5% CO2 for an hour. The C2C12 cells were subsequently re-fed for an hour with serum free no glucose DMEM with or without treatments. Then serum free media with and without treatment were removed and cells were incubated at 37 °C with 5% CO2 for a 30 min with 250 uM of fluorescent analogue of glucose, 6-(N-(7-Nitrobenz-2-oxa-l,3-diazol-4-yl)amino)-2-deoxyglucose (6-NBDG) in serum free no glucose

DMEM. The 6-NBDG uptake reaction was stopped by removing the incubation medium and washing the cells twice with pre-cold phosphate buffered saline (PBS pH-7.4). Cells in each well were subsequently re-suspended with pre-cold fresh optimum medium and maintained at 4°C for later fluorimetry analysis performed within 30 min. The experiments were conducted in 96-well plates. All the treatment groups were in triplicates. Example 2
[0067] Preparation of assay solution for synergistic effect of Cinnamomum cassia or cinnamon and Syzygium cumini or jamun extracts- Cinnamon and jamun extracts were used in the below mentioned ratio and concentration to study the synergistic effect on skeletal muscle cell glucose uptake assay by fluorimetry.
[0068] Figure 1, shows the uptake of glucose at various individual percentages of Cinnamomum cassia and Syzygium cumini extracts.
[0069] Figure 2, shows the increased uptake of glucose at specific combination of Cinnamomum cassia and Syzygium cumini extracts in the ratios of 1:1 (25 ug/ml of Cinnamomum cassia and Syzygium cumini extracts) and 1:3 (25 ug/ml of Cinnamomum cassia and 75 ug/ml of Syzygium cumini extracts) synergistically increase the percentage of glucose uptake in skeletal muscle cells when compared to individual ingredient alone (Figure 1). The percentage of increase of glucose uptake by Cinnamomum cassia extract alone at maximum concentration (50 ug/ml)

is 36-59. The percentage of increase of glucose uptake by Syzygium cumini extract alone at maximum concentration (50 ug/ml) is 35 to 53 and synergistic increase in percentage of glucose uptake by the combination comprising Cinnamomum cassia and Syzygium cumini extracts is 70 to 94 in the ratio of 1:1 (p=0.0009 with respect to Cinnamon extract alone; p=0.0007 with respect to Jamun extract alone). A similar synergy was observed for the composition comprising Cinnamomum cassia extract to Syzygium cumini extract weight ratio of 1:3. Example 3
[0070] Process for preparation of the composition: The composition was conveniently prepared by mixing commercially procured Cinnamomum cassia and Syzygium cumini extracts in a proportion, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5. [0071] The composition can be conveniently dispensed in the form of juices. In an implementation of the present disclosure, the extract powders in said composition can be added, but not restricted to, in amounts in the range of 0.25 - 2 gm in 100 ml of juices. Higher concentrates were taken in order to obtain juice concentrate. The concentrate was suitably diluted prior to use with water. Advantages of the present disclosure:
[0072] The present disclosure reveals an anti-diabetic composition comprising a) Cinnamomum cassia extract; and b) Syzygium cumini extract in specific weight ratios. The composition was found to enhance cellular glucose uptake, thus highlighting its value as a potential anti-diabetic supplement. The composition being herbal in nature can be regarded as a safe medication. The composition can be easily adapted to obtain useful commercial formulations such as health supplements, juices and concentrates.

I/We Claim:
1. An anti-diabetic composition comprising:
a) Cinnamomum cassia extract; and
b) Syzygium cumini extract,
wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is in a range of 1:0.5 to 1:3.5.
2. The anti-diabetic composition as claimed in claim 1, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:1.
3. The anti-diabetic composition as claimed in claim 1, wherein the Cinnamomum cassia extract to the Syzygium cumini extract weight ratio is 1:3.
4. The anti-diabetic composition as claimed in claim 1, wherein the Cinnamomum cassia extract comprises polyphenols having a weight percentage in a range of 20 - 30 % with respect to the extract.
5. The anti-diabetic composition as claimed in claim 1, wherein the Syzygium cumini extract comprises flavonoids having a weight percentage in a range of 3 - 10 % with respect to the extract.
6. The anti-diabetic composition as claimed in any one of the claims 1-5, wherein the composition comprises:

a) Cinnamomum cassia extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition; and
b) Syzygium cumini extract having a weight percentage in a range of 0.00125 -0.005% with respect to the composition.

7. The anti-diabetic composition as claimed in any one of the claims 1-6, wherein the composition further comprises at least one additive selected from the group consisting of carrier, chelating agent, coloring agent, flavoring agent, filler and preservative and combinations thereof.
8. A process for preparing the anti-diabetic composition as claimed in claim 1, said process comprising the steps of: a) obtaining Cinnamomum cassia extract; b) obtaining Syzygium cumini extract; and c) contacting the

Cinnamomum cassia extract with the Syzygium cumini extract to obtain the composition.
9. A process for preparing the anti-diabetic composition as claimed in claim 7, said process comprising the steps of: a) obtaining the Cinnamomum cassia extract; b) obtaining the Syzygium cumini extract; c) obtaining the at least one additive; and d) contacting the Cinnamomum cassia extract and the Syzygium cumini extract with the at least one additive to obtain the composition.
10. The anti-diabetic composition as claimed in any one of the claims 1-7, wherein the composition is dispensed in the form of a dietary supplement selected from the group consisting of juices, shakes, vegetable blends, fruit blends, concentrates, and combinations thereof.