Claims:I Or We Claim
1. An anti-microbial composition for achieving High Level Disinfection of a surface to which it is applied, the composition comprising of:
a quaternary ammonium biocide in the concentration range of 0.1 to 0.5%;
an essential oil in the concentration range of 0.1 to 0.2 %;
a non-ionic surfactant in the concentration range of 0.1 to 1%;
a naturally obtained organic acid in the concentration range of 0.1 to 0.5 %;
a distilled water along with an at least one pharmaceutically accepted excipients.
2. The anti-microbial composition as claimed in claim 1, wherein, the quaternary ammonium biocide is benzalkonium halide, which is selected from but not limited to benzalkonium chloride, dodecyl dimethyl ammonium chloride, and alkyl dimethyl benzyl ammonium chloride.
3. The anti-microbial composition as claimed in claim 1, wherein, the essential oil is selected from but not limited to basil oil, angelica root oil, aniseed oil, arnica blossom oil, eucalyptus oil, fennel oil, spruce alder oil, galbanum oil, geranium oil, and ginger grass oil.
4. The anti-microbial composition as claimed in claim 1, wherein, the non-ionic surfactants are selected from but not limited to caprylyl/capryl Glucoside, Coco glucoside, decyl glucoside, Lauryl glucoside, and sucrose cocoate.
5. The anti- microbial composition as claimed 1, wherein, the organic acid is selected from but not limited to citric acid, lactic acid, acetic acid and formic acid.
6. The method to achieve high level Disinfection of a surface, the method comprising of the steps:
applying the antimicrobial composition as claimed in claim 1 to the treating surface;
exposing the treating surface to the light having a wavelength in the range of 300-700 nm.
7. The treating surface as claimed in claim 6, wherein the treating surface is selected from but not limited to glassware and articles, kitchen furnishings and utensils, such as, preferably, ovens, glass ceramic hobs, hobs, kitchen furniture, kitchen fittings, porcelain items, ceramics, grills (including garden barbecues).
8. The application of the anti-microbial composition as claimed in claim 1, the application of the anti-microbial composition on the treating surface is selected from but not limited to brushing, rubbing, gluing, spraying, wiping or in particular by spraying, preferably by means of high-pressure cleaner.
, Description:FIELD OF THE INVENTION
The present invention relates to an anti-microbial composition used for achieving High-Level Disinfection of a surface to which it is applied. It particularly relates to an antimicrobial composition providing sustained antimicrobial efficacy.
BACKGROUND OF THE INVENTION
Microbes exist everywhere in the modern world. While some are beneficial to humans and the environment, others may have significant negative consequences for contaminated articles as well as the persons, animals and ecological members coming in contact with them. There are several industries and environments where such microbes are especially prevalent.
Human health is impacted by a variety of microbes such as protozoans, bacteria, fungi, molds and viruses. For example, invasion by microbial entities including various viruses and bacteria causes a wide variety of diseases and ailments. To reduce the effects of such an invasion, people frequently wash their skin with antimicrobial soaps.
Antibacterial soaps typically include soap, which is sodium or potassium salt of fatty acids, in combination with one or more antimicrobial agents like triclosan. It is very important to keep the worktops clean as they directly touch the food you eat. So if they are left dirty, the chances of the food we eat to be contaminated are high. Dinner plates, glasses, bowls, knives should be timely cleaned. These are high touch surfaces and not cleaning them timely can be a cause of spreading germs throughout the house. We should always clean the utensils before and after use not just by wiping with a cloth but cleaning them thoroughly with a disinfectant. Utensils like knives, wooden spatulas, tongs, and bowls should be disinfected to stop the spread of bacteria via food. It is necessary to keep our dishes clean and free of bacteria and virus type microbes to prevent ourselves from being ill.
In the present market, there are a variety of soap bars available for dishwashing. However, these lack either efficacy or are harsh or both. Companies focus on ways to improve the main three aspects of the dishwasher: improve its efficacy, aroma, and harshness of the bars or solutions thereof. A lot of research has been carried out and documents have been patented of which few are listed below.
WO2017089104A1 discloses an antimicrobial composition, typically including a soap with one or more antimicrobial agents like triclosan and an aromatic agent containing tulsi oil are capable of exhibiting a synergistic antimicrobial activity.
US2018072969A1 discloses an organic detergent powder and single-dose compressed tablets with organic ingredients like soapberry powder and one or more enzymes and fragrances.
US2006134239A1 discloses an organic composition that inhibits the asexual propogation of fungi and adhesion of microorganisms to the surface containing agents against the microorganism, for example patchouli oil and its derivatives.
The big drawback of a covered storage place for the bar soaps to avoid bacterial growth and other reasons like the addition of alcohol and low efficacy are the reasons for the need for an effective dishwasher that can satisfy all. Thus, the need for the present invention arises that can overcome the problems associated with the existing prior art.

OBJECTIVE OF THE INVENTION
The main objective of the present invention is to provide an anti-microbial composition for achieving High-Level Disinfection of a surface.
Yet another objective of the present invention is to provide a formulation that is able to confer a residual biocidal activity to the treating surfaces.
Yet another objective of the present invention is to provide an effective microbial reduction for an extended period of time after application.
Yet another objective of the present invention is to provide a cost-effective across a wide range of applications.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example.
SUMMARY OF THE INVENTION
The present invention relates to an anti-microbial composition used for achieving High Level Disinfection of a surface to which it is applied. The antimicrobial composition comprising of a quaternary ammonium biocide in the concentration range of 0.1 to 0.5%, an essential oil in the concentration range of 0.1 to 0.2 %, a non-ionic surfactant in the concentration range of 0.1 to 1%, a naturally obtained organic acid in the concentration range of 0.1 to 0.5 %, and a distilled water along with an at least one pharmaceutically accepted excipients. Herein, the quaternary ammonium biocide is benzalkonium halide, which is selected from but not limited to benzalkonium chloride, dodecyl dimethyl ammonium chloride, and alkyl dimethyl benzyl ammonium chloride. Herein, the essential oil is selected from but not limited to basil oil, angelica root oil, aniseed oil, arnica blossom oil, eucalyptus oil, fennel oil, spruce alder oil, galbanum oil, geranium oil, and ginger grass oil. Herein, the non-ionic surfactants are selected from but not limited to caprylyl/capryl Glucoside, Coco glucoside, decyl glucoside, Lauryl glucoside, and sucrose cocoate. Herein, the organic acid is selected from but not limited to citric acid, lactic acid, acetic acid and formic acid. In an embodiment of the present invention, the method to achieve high level disinfection of a surface is described. The method comprising of the following steps: applying the antimicrobial composition to the treating surface and then exposing the treated surface to the light having a wavelength in the range of 300-700 nm. In an embodiment of the present invention, the treating surface is selected from but not limited to glassware and articles, kitchen furnishings and utensils, such as, preferably, ovens, glass ceramic hobs, hobs, kitchen furniture, kitchen fittings, porcelain items, ceramics, grills (including garden barbecues). In an embodiment of the present invention, the application of the anti-microbial composition on the treating surface is selected from but not limited to brushing, rubbing, gluing, spraying, wiping or in particular by spraying, preferably using high-pressure cleaner.
The main advantage of the present invention is that the present invention provides an anti-microbial composition for achieving High Level Disinfection of a surface.
Yet another advantage of the present invention is that the present invention provides a formulation that is able to confer a residual biocidal activity to the treating surfaces.
Yet another advantage of the present invention is that the present invention provides an effective microbial reduction for an extended period of time after application.
Yet another advantage of the present invention is that the present invention is cost-effective across a wide range of applications.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein below, in which various embodiments of the disclosed invention are illustrated by way of example.

DETAILED DESCRIPTION OF THE INVENTION
Definition
The term “a” or “an”, as used herein, is defined as one. The term “plurality”, as used herein, is defined as two as or more than one. The term “another”, as used herein, is defined as at least a second or more. The terms “including” and/or “having”, as used herein, are defined as comprising (i.e., open language).
The term “comprising” is not intended to limit the present invention with such terminology rather is used in a wider sense. Any invention using the term comprising could be separated into one or more claims using “consisting” or “consisting of”. The term “comprising” may be used interchangeably with the terms “having” or “containing”.
Reference in this document to “one embodiment”, “certain embodiments”, “an embodiment”, “another embodiment”, and “yet another embodiment” or similar terms, throughout the document means that a specific feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of such phrases in various places, this specification throughout are not necessarily all referring to the same embodiment. Furthermore, the specific features, structures, or characteristics are combined in any suitable manner in one or more embodiments without limitation.
The term “or” as used herein is to be interpreted as inclusive or meaning any one or more combinations. Therefore, “A, B or C” means any of the following: “A; B; C; A and B; A and C; B and C; A, B and C”. An exception to this definition will occur only when a combination of elements, functions, steps, or acts are in mutually exclusive, inherently.
As used herein, the term "one or more" generally refers to, but is not limited to, singular as well as the plural form of the term.
The drawings featured in the figures are to illustrate certain convenient embodiments of the present invention and are not to be considered as a limitation to that. Term "means" preceding a present participle of operation indicates the desired function for which there is one or more embodiments, i.e., one or more methods, for achieving the desired function and that one skilled in the art could select from these or their equivalent in view of the disclosure herein and use of the term "means" is not intended to be limiting.
The present invention relates to an anti-microbial composition used for achieving High Level Disinfection of a surface to which it is applied. The antimicrobial composition comprising of a quaternary ammonium biocide in the concentration range of 0.1 to 0.5%, an essential oil in the concentration range of 0.1 to 0.2 %, a non-ionic surfactant in the concentration range of 0.1 to 1%, a naturally obtained organic acid in the concentration range of 0.1 to 0.5 %, and a distilled water along with an at least one pharmaceutically accepted excipients.
Herein, the quaternary ammonium biocide is benzalkonium halide, which is selected from but not limited to benzalkonium chloride, dodecyl dimethyl ammonium chloride, and alkyl dimethyl benzyl ammonium chloride. Herein, the essential oil is selected from but not limited to basil oil, angelica root oil, aniseed oil, arnica blossom oil, eucalyptus oil, fennel oil, spruce alder oil, galbanum oil, geranium oil, and ginger grass oil. Herein, the non-ionic surfactants are selected from but not limited to caprylyl/capryl Glucoside, Coco glucoside, decyl glucoside, Lauryl glucoside, and sucrose cocoate. Herein, the organic acid is selected from but not limited to citric acid, lactic acid, acetic acid and formic acid.
In an embodiment of the present invention, the method to achieve high level disinfection of a surface is described. The method comprising the following steps:
a. applying the antimicrobial composition to the treating surface;
b. exposing the treating surface to the light having a wavelength in the range of 300-700 nm.
In an embodiment of the present invention, the treating surface is selected from but not limited to glassware and articles, kitchen furnishings and utensils, such as, preferably, ovens, glass ceramic hobs, hobs, kitchen furniture, kitchen fittings, porcelain items, ceramics, grills (including garden barbecues).
In an embodiment of the present invention, the application of the anti-microbial composition on the treating surface is selected from but not limited to brushing, rubbing, gluing, spraying, wiping or in particular by spraying, preferably by means of high-pressure cleaner.
In an aspect of the present invention, the treated material disclosed herein is utensils, wherein the utensils are are made up of materials like glass, porcelain, melamine, stainless steel etc.
In another aspect of the present invention, the treated materials, herein, utensils are washed with soaps and then finally rinsed with water. Water is generally tap water and have some bioload. After rinsing the utensils, they are wiped with a kitchen towel. After wiping the utensils, they are kept in racks or shelves and are used to serve food after some days also.

Antimicrobial efficiency & longevity of the present invention against E.Coli, Salmonella spp. and Staphylococcus aureus after freshly spraying and after Fresh (untreated), 12h, 24h, 48h and 72h (treated) on hard surfaces like Stainless Steel, Melamine and Porcelain surface is performed.
Test Organism: (Bacterial Strains)
1. Escherichia coli ATCC 8739
2. Staphylococcus aureus ATCC 6538
3. Salmonella spp. ATCC 13311
Experiment Details
Incubation conditions: 37°C for 12, 24, 48, 72 hours
Test Surface: Stainless Steel, Melamine and Porcelain (5 x 5 cm)
Neutralizing medium: SCDM containing Lecithin and Tween 80
Test Procedure
Bacterial cells with a cell density of 2.5×105 to 10×105 cells/ml were added to each surface with a volume of 0.4ml/surface added separately on each surface in order to achieve the bacterial concentration of 6200 to 25000 cells/cm2. Then the surfaces were exposed with test sample. The inoculum added in 0-hour surface (untreated) was washed out immediately with 10 mL of SCDLP broth and the washed-out solutions inoculated in agar plates and incubated at 35±1°C. Similarly, the surfaces meant for 12, 24, 48, 72 hours were incubated for 24 hours at 35±1°C after the addition of inoculum on the surface. After 12, 24, 48, 72 hours the surfaces were washed out with SCDLP broth, the dilutions were inoculated in agar plates and incubated for 24 hours. The number of colonies in each plate for both time points was observed after 24 hours of incubation.

Results
Sample Description Present invention
Contact Time 12 h, 24 h, 48 h, 72 h
Test Organism Duration Melamine Porcelain Stainless steel

E. coli 12 h 99.04% 99.39% 99.61%
24 h 99.86% 99.53% 99.07%
48 h 98.14% 99.30% 98.76%
72 h 97.05% 99.46% 97.98%

Staphylococcus aureus 12 h 99.04% 99.16% 99.54%
24 h 97.24% 97.30% 97.10%
48 h 88.98% 95.84% 91.16%
72 h 86.61% 94.29% 90.24%

Salmonella spp. 12 h 99.00% 99.07% 99.11%
24 h 98.93% 99.68% 93.99%
48 h 97.00% 97.32% 92.45%
72 h 93.75% 95.03% 90.34%

Staphylococcus aureus
Fresh (Untreated)
Melamine
Porcelain
Stainless steel
Uo (Log10) 3.07 3.07 3.06
Ut (Log10) 2.92 2.86 2.88
Replicates (At) 12 h 24 h 48 h 72 h 12 h 24 h 48 h 72 h 12 h 24 h 48 h 72 h
R1 8 22 87 105 6 18 30 38 2 19 70 77
R2 8 24 80 98 6 23 33 44 5 25 64 71
Average 8 23 83.5 101.5 6 20.5 31.5 41 3.5 22 67 74
Log10 0.90 1.36 1.92 2.01 0.78 1.31 1.50 1.61 0.54 1.34 1.83 1.87
Reduction 2.02 1.49 0.96 0.87 1.87 1.38 1.57 1.24 2.34 1.54 1.05 1.01
% Reduction 99.04 97.24 88.98 86.61 99.16 97.30 95.84 94.29 99.54 97.10 91.16 90.24

Salmonella spp
Fresh (Untreated)
Melamine
Porcelain
Stainless steel
Uo (Log10) 3.04 3.06 3.07
Ut (Log10) 2.87 2.81 2.79
Replicates (At) 12 h 24 h 48 h 72 h 12 h 24 h 48 h 72 h 12 h 24 h 48 h 72 h
R1 7 6 20 43 5 1 18 34 4 39 49 57
R2 8 10 17 34 7 3 15 30 7 35 44 62
Average 7.5 8 18.5 38.5 6 2 16.5 32 5.5 37 46.5 59.5
Log10 0.88 0.90 1.27 1.59 0.78 0.30 1.22 1.51 0.74 1.57 1.67 1.77
Reduction 2.00 1.91 1.52 1.20 1.78 2.21 2.49 1.30 2.05 1.22 1.12 1.02
% Reduction 99.00 98.93 97.00 93.75 99.07 99.68 97.32 95.03 99.11 93.99 92.45 90.34

E. coli
Fresh (Untreated) Melamine Porcelin Stainless steel
Uo (Log10) 3.05 3.05 3.04
Ut (Log10) 2.86 2.82 2.81
Replicates(At) 12 h 24 h 48 h 72 h 12 h 24 h 48 h 72 h 12 h 24 h 48 h 72 h
R1 9 0 14 19 5 2 6 3 3 7 9 12
R2 5 2 10 19 3 4 3 4 2 5 7 14
Average 7 1 12 19 4 3 4.5 3.5 2.5 6 8 13
Log10 0.85 0.00 1.08 1.28 0.60 0.48 0.65 0.54 0.40 0.78 0.90 1.11
Reduction 2.02 2.82 1.73 1.53 1.98 2.10 1.92 2.27 2.41 2.03 1.91 1.69
% Reduction 99.04 99.86 98.14 97.05 99.39 99.53 99.30 99.46 99.61 99.07 98.76 97.98

Calculation of antibacterial activity When the test is determined to be valid, the antibacterial activity is calculated as per the formula mentioned below:
R = (Ut - Uo) - (At - Uo) = Ut – At
Where, R: antibacterial activity
Uo: mean of logarithm numbers of viable bacteria immediately after inoculation on untreated test pieces.
Ut: mean of logarithm numbers of viable bacteria after inoculation on untreated test pieces after 24h.
At: mean of logarithm numbers of viable bacteria immediately after inoculation on anti-bacterial test pieces after 12, 24, 48, 72h.
It is concluded from the results of this study that the anti-microbial composition disclosed in the present application exhibited in test organisms, 88.98 % to 99.54 % in Staphylococcus aureus, 90.34 % to 99.68 % in Salmonella spp., and 97.05 to 99.86 % in E.coli bacterial growth reduction over control under the test surfaces and conditions employed in a given contact time.
Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein, in which various embodiments of the disclosed present invention are illustrated by way of example and appropriate reference to accompanying drawings. Those skilled in the art to which the present invention pertains may make modifications resulting in other embodiments employing principles of the present invention without departing from its spirit or characteristics, particularly upon considering the foregoing teachings. Accordingly, the described embodiments are to be considered in all respects only as illustrative, and not restrictive, and the scope of the present invention is, therefore, indicated by the appended claims rather than by the foregoing description or drawings. Consequently, while the present invention has been described with reference to particular embodiments, modifications of structure, sequence, materials and the like apparent to those skilled in the art still fall within the scope of the invention as claimed by the applicant.