TECHNICAL FIELD
[001] The subject matter described herein in general relates to cleansing
compositions, and in particular, relates to the antimicrobial compositions.
5 BACKGROUND OF INVENTION
[002] Microorganisms have been known to co-exist with mankind. However, the relationship has led to grave risks. Microbial proliferation in our environment has led to an increase in microbe-assisted infections and diseases. Since, obtaining an adequately sterile environment is a distant dream, it is generally recognized that the
10 antimicrobial actives, such as preservatives, greatly reduces the growth and
proliferation of microorganisms when dosed into formulations. The usage of such anti-microbial actives may also lead to increased shelf-life of cosmetics and other consumer goods. For this purpose, effective antimicrobial actives are frequently employed in the cosmetic industry, and the like.
15 [003] Conventionally available preservatives that exist involve the use of
aggressive chemical agents like for example butylated hydroxy toluene, formaldehyde, alcohols, phenols, sodium azide. The major disadvantages associated with the use of these chemical preservatives is that they may be toxic, not bio-safe, corrosive; and can cause irritation on skin and mucous membrane of
20 the user. The use of herbal actives has distinct advantages of increased bio-
availability and high bio-compatibility.
[004] Furthermore, in the recent years, owing to environmental concerns, compositions derived from natural sources, such as essential oils, are being increasingly used as desirable alternatives in consumer products such as sanitizers,
25 and disinfectants. Essential oils, in contrast to conventionally used antimicrobials,
are completely dependent on and limited by the natural resources of the respective plants. For example, tea tree oil (TTO) from Melaleuca alternifolia is one prominent example of essential oils with good biocidal action against bacteria and fungi (Carson, C. F et al., Clinical Microbiology Reviews, Vol. 19, No. 1, 50-62).
30 Further, US 6767876B2 discloses a composition comprising surface cleaning
composition comprising Mentha Spicata var.viridis oil, citronella oil, 2-

ethoxyethanol, sodium hydroxide, benzalkonium chloride, and sodium lauryl
sulphate surfactant. Furthermore, U.S. Pat. No. 3,688,985 discloses emulsions of
essential oils, such as methyl salicylate, and thymol that are impregnated into water
insoluble resins. However, cleaning products have met with minimal acceptance in
5 the marketplace owing to poor cleansing effects and similar instances/symptoms of
skin irritancy experienced as with synthetic chemical agents.
[005] Considering the above evidence, there exists a need to develop a herbal
cleanser that is devoid of synthetic ingredients, that provides better cleansing
properties and proves safe to use. It is therefore an object of the invention to
10 provide antibacterial compositions that overcome the drawbacks as discussed in the
prior art.
SUMMARY OF THE INVENTION
[006] In an aspect of the present invention, there is provided an antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to
15 vanillin weight ratio is the range of 1:0.026 – 1:80.
[007] In an aspect of the present invention, there is provided an antibacterial formulation comprising: i) an antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80; and ii) at least one cosmetically suitable carrier.
20 [008] In another aspect of the present invention, there is provided a process for
preparing the antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80, said process comprising steps of: a) obtaining lavindin; b) obtaining vanillin; and c) contacting lavindin and vanillin to obtain the composition.
25 [009] These and other features, aspects, and advantages of the present subject
matter will be better understood with reference to the following description and appended claims. This summary is provided to introduce a selection of concepts in a simplified form. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit
30 the scope of the claimed subject matter.

DETAILED DESCRIPTION OF THE INVENTION
[0010] Those skilled in the art will be aware that the present disclosure is subject to
variations and modifications other than those specifically described. It is to be
understood that the present disclosure includes all such variations and
5 modifications. The disclosure also includes all such steps, features, compositions,
and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations of any or more of such steps or features. Definitions [0011] For convenience, before further description of the present disclosure,
10 certain terms employed in the specification, and examples are delineated here.
These definitions should be read in the light of the remainder of the disclosure and understood as by a person of skill in the art. The terms used herein have the meanings recognized and known to those of skill in the art, however, for convenience and completeness, particular terms and their meanings are set forth
15 below.
[0012] The articles “a”, “an” and “the” are used to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article.
[0013] The terms “comprise” and “comprising” are used in the inclusive, open sense, meaning that additional elements may be included. It is not intended to be
20 construed as “consists of only”.
[0014] Throughout this specification, unless the context requires otherwise the word “comprise”, and variations such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated element or step or group of element or steps but not the exclusion of any other element or step or group of element or
25 steps.
[0015] The term “including” is used to mean “including but not limited to”. “Including” and “including but not limited to” are used interchangeably. [0016] The term ‘MIC’ refers to minimum inhibitory concentration. MIC is the lowest concentration of an antimicrobial (like an antifungal, antibiotic or
30 bacteriostatic) drug that will inhibit the visible growth of a microorganism after
overnight incubation.

[0017] The term “liquid cleansing product” include shower gel, face wash, body
wash, hand wash, and paste. It is apparent that numerous other forms and
modifications of the liquid cleansing product will be obvious to those skilled in the
art and generally should be construed to cover all such obvious forms and
5 modifications which are within the true spirit and scope of the present disclosure.
[0018] The term “solid cleansing products” include cleansing bars, and films. It is apparent that numerous other forms and modifications of the solid cleansing product will be obvious to those skilled in the art and generally should be construed to cover all such obvious forms and modifications which are within the true spirit
10 and scope of the present disclosure.
[0019] Ratios, concentrations, amounts, and other numerical data may be presented herein in a range format. It is to be understood that such range format is used merely for convenience and brevity and should be interpreted flexibly to include not only the numerical values explicitly recited as the limits of the range, but also
15 to include all the individual numerical values or sub-ranges encompassed within
that range as if each numerical value and sub-range is explicitly recited. For example, concentration ranges of about 0.002-0.15 % should be interpreted to include not only the explicitly recited limits of about 0.002 % to about 0.15 %, but also to include sub-ranges, such as 0.002-0.1 %, 0.0022-0.15 %, and so forth, as
20 well as individual amounts, including fractional amounts, within the specified
ranges.
[0020] The term “at least one” is used to mean one or more and thus includes individual components as well as mixtures/combinations. [0021] A composition comprising “synergistic activity” or a “synergistic
25 composition” is a combination of compounds which exhibits increased biological
or functional activity as a non-linear multiple of the biological or functional activity of the individual compounds. In other words, the combined biological or functional activity of two or more compounds being tested is significantly greater than the expected result based on independent effects of the compounds when
30 tested separately. Synergy may be apparent only at some ranges or weight
percentages.

[0022] Carriers are substances that serve as mechanisms to improve the delivery and the effectiveness of drugs
[0023] A diluent (also referred to as filler, diluent or thinner) is a diluting agent.
[0024] Diluent is an inactive substance that serves as the vehicle or medium for a
5 drug or other active substance.
[0025] Lavindin oil is oil extracted from plant Lavindin. Lavindin is born as a result of the hybridization of Lavandula angustifolia and Lavandula latifolia. The main components of this oil are lavandulol, linalool, linalyl acetate, camphor, cineole, caryophyllene, camphene, dipentene, limonene, ocimene, and terpinene.
10 Any plant part of lavindin such as leaves, seed, bark, root, flowers, fruit, or any
other plant part can be used to extract lavindin oil.
[0026] Vanillin is a phenolic aldehyde, which is an organic compound with the molecular formula C8H8O3. It is found in Leptotes bicolor, a species of orchid native to Paraguay and southern Brazil, and the Chinese red pine. Any plant part
15 such as leaves, seed, bark, root, flowers, fruit, or any other plant part can be used to
extract vanillin.
[0027] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or
20 equivalent to those described herein can be used in the practice or testing of the
disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference.
[0028] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of
25 exemplification only. Functionally-equivalent products, compositions, and methods
are clearly within the scope of the disclosure, as described herein.
[0029] As mentioned previously, conventionally used chemical biocides are toxic, not bio-safe, corrosive; and cause irritation on skin and mucous membrane of the user. Although essential oils obtained from natural sources is increasingly used as a
30 desirable alternative for preserving/cleansing/sanitizing/disinfecting surfaces, so far
these cosmetic products have met with minimal acceptance in the marketplace

owing to poor sensorial and similar instances/symptoms of skin irritancy
experienced as with synthetic chemical agents. The present disclosure aims to solve
the said problem by using a synergistic combination of lavindin oil and vanillin.
[0030] In an embodiment of the present invention, there is provided an
5 antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein
lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80. In another embodiment of the present disclosure, lavindin oil to vanillin weight ratio is in the range of 1:0.05 – 1:70. In yet another embodiment of the present disclosure lavindin oil to vanillin weight ratio is in the range of 1:0.05 - 62.
10 [0031] In an embodiment of the present disclosure, there is provided an
antibacterial composition described herein comprising: wherein lavindin oil has a weight percentage in range of 0.002 to 0.15% with respect to the composition. In another embodiment, the lavindin oil has a weight percentage in the range of 0.002 - 0.07 % with respect to said composition.
15 [0032] In an embodiment of the present disclosure, there is provided an
antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80, and wherein lavindin oil has a weight percentage in range of 0.002 to 0.15% with respect to the composition.
20 [0033] In an embodiment of the present disclosure, there is provided an
antibacterial composition described herein comprising: wherein vanillin has a weight percentage in range of 0.004 – 0.16 % with respect to the composition. In another embodiment, vanillin has a weight percentage in the range of 0.0044 -0.158 % with respect to said composition.
25 [0034] In an embodiment of the present disclosure, there is provided an
antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80, and wherein vanillin has a weight percentage in range of 0.004 – 0.16 % with respect to the composition.
30 [0035] In an embodiment of the present disclosure, there is provided an
antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein

lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80, and wherein
lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the
composition, and wherein vanillin has a weight percentage in range of 0.004 – 0.16
% with respect to the composition.
5 [0036] In an embodiment of the present disclosure, there is provided a formulation
comprising: i) the composition comprising: a) lavindin oil, and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80; and ii) at least one cosmetically suitable carrier. [0037] In an embodiment of the present disclosure, there is provided a formulation
10 comprising: i) the composition comprising: a) lavindin oil, and b) vanillin, wherein
lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80; and wherein lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the composition; and ii) at least one cosmetically suitable carrier. [0038] In an embodiment of the present disclosure, there is provided a formulation
15 comprising: i) the composition comprising: a) lavindin oil, and b) vanillin, wherein
lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80; and wherein lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the composition, and wherein vanillin has a weight percentage in range of 0.004 – 0.16 % with respect to the composition; and ii) at least one cosmetically suitable carrier.
20 [0039] In an embodiment of the present disclosure, there is provided a formulation
as described herein, wherein the at least one cosmetically suitable carrier comprises additives selected from the group consisting of diluent, anti-caking agent, absorbent, skin protectant, viscosity modifier, opacifying agent, preservative, skin conditioning agent, cooling agent, odour enhancer, hydrophilic polymer, UV
25 stabilizer, pH adjusting agent, chelating agent, deodorant, perfumes, antimicrobial,
antioxidant, humectant, conditioning ingredients, propellants, salts, colorants, dyes, and combinations thereof. A list of additives has been provided, however the provided list is not being considered exhaustive and other known additives may be considered to fall within scope of the present disclosure.
30 [0040] In an embodiment of the present disclosure, there is provided a formulation
comprising: a) lavindin oil having a weight percentage in range of 0.002–0.15 %

with respect to the composition; b) vanillin having a weight percentage in range of
0.004 – 0.15 % with respect to the composition, c) anti-caking agent having a
weight percentage in the range of 60 – 70% with respect to the composition; d)
absorbent having a weight percentage in the range of 10 – 15% with respect to the
5 composition; e) skin protectant having a weight percentage in the range of 10 –
15% with respect to the composition; f) viscosity modifiers having a weight percentage in the range of 0 – 20% with respect to the composition; g) opacifying agent having a weight percentage in the range of 0 – 10% with respect to the composition; h) preservative having a weight percentage in the range of 0 – 5%
10 with respect to the composition; i) skin conditioning agent having a weight
percentage in the range of 0 – 1.5% with respect to the composition; j) cooling agent having a weight percentage in the range of 0 – 2% with respect to the composition; k) odour enhancer having a weight percentage in the range of 0.5 – 3% with respect to the composition; l) perfume having a weight percentage in the
15 range of 0.5 – 30% with respect to the composition; m) antioxidant having a weight
percentage in the range of 0.001 – 5% with respect to the composition; and n) chelating agent having a weight percentage in the range of 0.001 – 0.2% with respect to the composition, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80.
20 [0041] In an embodiment of the present disclosure, there is provided an
antibacterial formulation as described herein, wherein a) anti-caking agent is talc; b) absorbent is starch; c) skin protectant is selected without limitation from the group consisting of zinc oxide, zinc carbonate, and combinations thereof; d) viscosity modifier is selected without limitation from the group consisting of
25 aluminium starch octenylsuccinate, crosslinked polyacrylate polymers, carboxylic
acid polymers, polyacrylamide polymers, acrylic acid/ethyl acrylate copolymers, carboxyvinyl polymers, polyalkenyl polyether crosslinked polymer of acrylic acid crosslinked, and mixtures thereof; e) opacifying agent is selected without limitation from the group consisting of titanium dioxide, diatomaceous earth, calcium
30 carbonate, magnesium carbonate, and combinations thereof; f) preservative is
selected without limitation from the group consisting of boric acid, capryloyl

salicylic acid, and combinations thereof; g) skin conditioning agent is selected
without limitation from the group consisting of salicylic acid, allantoin, calcium
salicylate, magnesium salicylate, potassium salicylate, sodium salicylate,
panthenol, panthetine, pantotheine, panthenyl ethyl ether, and combinations
5 thereof; h) cooling agent is selected without limitation from the group consisting of
menthol, trimethyl isopropyl butanamide, peppermint oil, and combinations thereof; i) odour enhancer is selected from known fragrances; j) perfume is selected without limitation from known fragrances; k) hydrophilic polymer is selected from group consisting of polyethylene glycols (PEGs), polyvinylpyrrolidones (PVP),
10 hydroxypropyl methylcellulose (HPMC), poloxamers, and combinations thereof; l)
UV stabilizer is selected without limitation from group consisting of benzophenone-3 and other known UV stabilizers in the art; m) antioxidants is selected without limitation from group consisting of tocopheryl acetate, propyl, octyl and dodecyl esters of gallic acid, butylated hydroxyanisole (BHA, usually
15 purchased as a mixture of ortho and meta isomers), butylated hydroxytoluene
(BHT) , nordihydroguaiaretic acid, oxynex (Oxynex ST liquid is a mixture of diethylhexyl syringyliden-emalonate and caprylic/capric triglyceride), vitamin A, vitamin E, vitamin C, and other known antioxidants in the art; n) preservative is selected without limitation from group consisting of phenoxyethanol, benzyl
20 alcohol, methyl paraben, propyl paraben, and combinations thereof; o) pH
adjusting agent is selected without limitation from group consisting of lactic acid, citric acid, sodium citrate, succinic acid, phosphoric acid, sodium hydroxide, sodium carbonate, and combinations thereof; q) antimicrobial is selected without limitation from group consisting of farnesol, zinc phenolsulphonate,
25 ethylhexylglycerin, and combinations thereof; r) humectant is selected without
limitation from group consisting of tribehenin, glycerine, and combinations thereof; s) propellant is selected without limitation from group consisting of propane, isopropane, butane, isobutene, and combinations thereof; t) salt is selected without limitation from group consisting of potassium acetate, sodium chloride, and
30 mixtures thereof; u) colorant and dye are selected from group consisting of known
colorants in the art; v) chelating agent is selected without limitation from group

consisting of ethylene diaminetetraacetic acid (EDTA) , EDTA disodium, calcium
disodium edetate, EDTA trisodium, citric acid, EDTA tetrasodium, EDTA
dipotassium, and combinations thereof; and w) diluent is selected from group
consisting of water, alcohol, silicone, oil, and combinations thereof. In another
5 embodiment of the present disclosure, the absorbent is a starch selected from corn
starch, maize starch, and other known starches. In yet another embodiment of the present disclosure, the viscosity modifier is selected from well-known compounds as mentioned in Amjad et al., Carbomer Resins: Past, Present and Future Cosmetics & Toiletries 107 (1992), pp 81-85. In an alternate embodiment of the present
10 disclosure, the viscosity modifier is selected from resins consisting of a colloidally
water-soluble polyalkenyl polyether crosslinked polymer of acrylic acid crosslinked with from 0.75% to 2.00% of a crosslinking agent such as for example polyallyl sucrose or polyallyl pentaerythritol. [0042] In an embodiment of the present disclosure, there is provided an
15 antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein
lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein the said composition inhibits growth of Gram-negative bacteria.
[0043] In an embodiment of the present disclosure, there is provided an antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein
20 lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein
lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the composition, and wherein the said composition inhibits growth of Gram-negative bacteria. [0044] In an embodiment of the present disclosure, there is provided an
25 antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein
lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 75; and wherein vanillin has a weight percentage in range of 0.004 – 0.16 % with respect to the composition; and wherein the said composition inhibits growth of Gram-negative bacteria.
30 [0045] In an embodiment of the present disclosure, there is provided an
antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein

lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein
lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the
composition, and wherein vanillin has a weight percentage in range of 0.004 – 0.16
% with respect to the composition; and wherein the said composition inhibits
5 growth of Gram-negative bacteria.
[0046] In an embodiment of the present disclosure, there is provided an antibacterial formulation comprising: i) a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and ii) at least one cosmetically suitable carrier; and wherein the said formulation inhibits growth
10 of Gram-negative bacteria.
[0047] In an embodiment of the present disclosure, there is provided an antibacterial formulation comprising: i) a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the
15 composition, and wherein vanillin has a weight percentage in range of 0.004 – 0.16
% with respect to the composition, and ii) at least one cosmetically suitable carrier; and wherein the said formulation inhibits growth of Gram-negative bacteria. [0048] In an embodiment of the present disclosure, there is provided an antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein
20 lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein the
said composition inhibits growth of gram-positive bacteria.
[0049] In an embodiment of the present disclosure, there is provided an antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein
25 lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the
composition, and wherein the said composition inhibits growth of gram-positive bacteria.
[0050] In an embodiment of the present disclosure, there is provided an antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein
30 lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein
vanillin has a weight percentage in range of 0.004 – 0.16 % with respect to the

composition; and wherein the said composition inhibits growth of gram-positive bacteria.
[0051] In an embodiment of the present disclosure, there is provided an
antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein
5 lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein
lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the composition, and wherein vanillin has a weight percentage in range of 0.004 – 0.16 % with respect to the composition; and wherein the said composition inhibits growth of gram-positive bacteria.
10 [0052] In an embodiment of the present disclosure, there is provided an
antibacterial formulation comprising: i) a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and ii) at least one cosmetically suitable carrier; and wherein the said formulation inhibits growth of gram-positive bacteria.
15 [0053] In an embodiment of the present disclosure, there is provided an
antibacterial formulation comprising: i) a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1: 80; and wherein lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the composition, and wherein vanillin has a weight percentage in range of 0.004 – 0.16
20 % with respect to the composition, and ii) at least one cosmetically suitable carrier;
and wherein the said formulation inhibits growth of gram-positive bacteria. [0054] In an embodiment of the present disclosure, there is provided a process for preparing the antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80, said
25 process comprising steps of: a) obtaining lavindin oil; b) obtaining vanillin; and c)
contacting lavindin oil with vanillin to obtain the composition.
[0055] In an embodiment of the present disclosure, there is provided a process for preparing the antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80, and
30 wherein lavindin oil has a weight percentage in range of 0.002 – 0.15 % with
respect to the composition, said process comprising steps of: a) obtaining lavindin

oil; b) obtaining vanillin; and c) contacting lavindin oil with vanillin to obtain the composition.
[0056] In an embodiment of the present disclosure, there is provided a process for
preparing the antibacterial composition comprising: a) lavindin oil; and b) vanillin,
5 wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80, and
wherein vanillin has a weight percentage in range of 0.004 – 0.16 % with respect to the composition, said process comprising steps of: a) obtaining lavindin oil; b) obtaining vanillin; and c) contacting lavindin oil with vanillin to obtain the composition.
10 [0057] In an embodiment of the present disclosure, there is provided a process for
preparing the antibacterial composition comprising: a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80, and wherein lavindin oil is having weight percentage in range of 0.002 – 0.15 % with respect to the composition, and wherein vanillin has a weight percentage in range
15 of 0.004 – 0.16 % with respect to the composition, said process comprising steps
of: a) obtaining lavindin oil; b) obtaining vanillin; and c) contacting lavindin oil with vanillin to obtain the composition.
[0058] In an embodiment of the present disclosure, there is provided a process for preparing the antibacterial composition comprising: a) lavindin oil; and b) vanillin,
20 wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80; ii) and at
least one cosmetically suitable carrier; said process comprising the steps of: a) obtaining lavindin oil; b) obtaining vanillin; and c) contacting lavindin oil and vanillin with at least one cosmetically suitable carrier to obtain the composition. [0059] In an embodiment of the present disclosure, there is provided a process for
25 preparing a formulation comprising: i) the composition comprising: a) lavindin oil,
and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80; and ii) and at least one cosmetically suitable carrier comprising additives selected from the group consisting of diluent, anti-caking agent, absorbent, skin protectant, viscosity modifier, opacifying agent, preservative, skin conditioning
30 agent, cooling agent, odour enhancer, hydrophilic polymer, UV stabilizer, pH
adjusting agent, chelating agent, deodorant, perfumes, antimicrobial, antioxidant,

humectant, conditioning ingredients, propellants, salts, colorants, dyes, and
combinations thereof, said process comprising the steps of: a) obtaining lavindin
oil; b) obtaining vanillin; and c) contacting lavindin oil and vanillin with at least
one cosmetically suitable carrier to obtain the composition.
5 [0060] In an embodiment of the present disclosure, there is provided a process for
preparing an antimicrobial composition, said process comprising the steps of i) obtaining an antibacterial composition as described herein, wherein said composition comprising a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80; ii) contacting the composition
10 with personal care product or a household product, wherein contacting prevents the
product from being contaminated by microorganisms. .
[0061] In an embodiment of the present disclosure, there is provided a process for preparing an antimicrobial composition, said process comprising the steps of i)
15 obtaining an antibacterial composition as described herein, wherein said
composition comprising a) lavindin oil; and b) vanillin, wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 - 1:80; and wherein lavindin oil has a weight percentage in range of 0.002 – 0.15 % with respect to the composition; ii) contacting the composition with personal care product or a household product,
20 wherein contacting prevents the product from being contaminated by
microorganisms.
[0062] In an embodiment of the present disclosure, there is provided a process for preparing an antimicrobial composition, said process comprising the steps of i) obtaining an antibacterial composition as described herein, wherein said
25 composition comprising a) lavindin oil; and b) vanillin, wherein lavindin oil to
vanillin weight ratio is the range of 1:0.026 - 1:80; and wherein vanillin has a weight percentage in range of 0.004 – 0.15 % with respect to the composition; ii) contacting the composition with personal care product or a household product, wherein contacting prevents the product from being contaminated by
30 microorganisms.

[0063] In an embodiment of the present disclosure, there is provided a process for
preparing an antimicrobial composition, said process comprising the steps of i)
obtaining an antibacterial composition as described herein, wherein said
composition comprising a) lavindin oil; and b) vanillin, wherein lavindin oil to
5 vanillin weight ratio is the range of 1:0.026 - 1:80; and wherein lavindin oil has a
weight percentage in range of 0.002 – 0.15 % with respect to the composition; and wherein vanillin has a weight percentage in range of 0.004 – 0.15 % with respect to the composition; ii) contacting the composition with personal care product or a household product, wherein contacting prevents the product from being
10 contaminated by microorganisms.
[0064] In an embodiment of the present disclosure, there is provided a formulation as described herein, wherein the composition is dispensed in a form selected from powders, shower gels, sprays, emulsion, solution, antiseptics, patches, face washes, body washes, hand washes, pastes, liquid cleansing products, solid cleansing
15 products, cleansing bars and films. In another embodiment of the present
disclosure, the formulation is dispensed in the form of powder.
EXAMPLES
[0065] The disclosure will now be illustrated with working examples, which is
intended to illustrate the working of disclosure and not intended to take
20 restrictively to imply any limitations on the scope of the present disclosure. Unless
defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and
25 compositions, the exemplary methods, devices and materials are described herein.
It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may apply. [0066] Conventionally used chemical biocides are toxic, not bio-safe, corrosive; and cause irritation on skin and mucous membrane of the user. Although essential
30 oils obtained from natural sources is increasingly used as a desirable alternative for
cleansing/sanitizing/disinfecting surfaces, so far these cleaning products have met

with minimal acceptance in the marketplace owing to poor cleansing effects and similar instances/symptoms of skin irritancy experienced as with synthetic chemical agents. The present disclosure aims to solve the said problem by using a synergistic combination of lavindin oil and vanillin. 5 Materials Required
[0067] Sterile Muller Hinton broth media (bacteria), sabouraud dextrose broth (fungi), sterile round bottom 96 wells of the microtitre plate transparent, Microbial Culture, alamar blue dye/ resazurin. Lavindin oil is a value-added essential oil that was procured from well-known commercial sources. Vanillin is a synthetic 10 compound procured from well-known commercial sources.
Bacterial Cultivation: -E. coli & S. aureus
[0068] E. coli & S. aureus were cultured on sterile Muller Hinton broth media, and the plates were incubated at 37° C for 24 hours under aerobic condition. The inoculum size was adjusted to 0.1 absorbance at 600 nm (corresponding to 15 approximately 108 CFU/mL) using spectrophotometer. The culture was further diluted by 100-fold in Sterile Muller Hinton broth to a concentration of approximately 106 CFU/ml.
Sample/Active Preparation:
[0069] Test Sample A (TSA) i.e., lavindin oil, was prepared at a concentration 20 128 times greater than the expected Minimum Inhibitory Concentration (MIC) values.
[0070] Test Sample B (TSB) i.e., vanillin, was prepared at a concentration 64 times greater than the expected MIC values.
Minimum Inhibitory Concentration (MIC) determination 25 [0071] Individual MIC values were determined for each test sample according to the microbroth dilution method as per CLSI M11-A7 Vol. 27 No.2 standard (modified). Briefly, serial dilutions of each test sample (50 μl) were added to wells in a 96-well plate followed by 50 μl of the prepared E. coli inoculum as described earlier. Media without E. coli and medium with E. coli only were used for negative 30 and positive controls, respectively, and contained the highest concentration of solvent used in each sample analysis. Similar procedure was adopted to determine

the MIC values for each test sample against S. aureus. Plates were incubated under
37⁰ C for 24 hours under aerobic conditions. Following incubation, each well was
re-suspended by pipetting and the optical density at 600 nm (OD600) was
determined on a plate reader. The MIC value was defined as the first well showing
5 > 95% reduction in bacterial growth compared to controls. The MIC values for
both lavindin oil and vanillin against E. coli and S. aureus are herewith presented below in Table 1.
10 [0072] Further to MIC observations, the starting concentration of two actives was selected for the checkerboard assay.
[0073] Checkerboard Assay as per Micro-Broth Dilution. As the invention is directed towards the substitution of prevalently used natural antibacterial agents, appraising the synergistic effect of combination of phytochemicals (lavindin oil
15 and vanillin) is essential. Thus, two-dimensional checkerboard assay was employed to assess the in vitro interactions between lavindin oil and vanillin using 96 well microtiter plate in 8 (rows) X 12 (columns) grid. 50μl of the nutrient broth was added to all the wells. 100μl of the broth was added in the 12th column which acts as a sterility control. Further, 50 μl of lavindin oil was added at 8x the desired final
20 concentration was added in the first column (A1 to H1) and serially diluted on the x-axis till the 10th column (A10 to H10) where 50μl of the solution was discarded to maintain same volume of the solution in all wells. Similarly, 50 μl of vanillin was added, at 4x the desired final concentration, in the row (A1 to A10), in which each row contains the same amount of vanillin being diluted on column (A to H).
25 The result is that each well in the checkerboard contains a unique combination of lavindin oil and vanillin being tested where that the highest concentration of lavindin oil and the highest concentration of vanillin were in the same well at one

corner of the 10 x 8 grid, and similarly, the lowest concentration of lavindin oil and vanillin was present at another corner of the plate. 100 μl microbial suspension (106 CFU/ml) of E. coli was further added to all the wells. Similar procedure is subsequently repeated to determine the effect of the combination against S. aureus. 5 A sterility control (A12 to H12), growth control (100 μl media + 100 μl bacterial cell suspension from A11 to H11) was set-up. The plates were incubated at 25°C for 24hours under aerobic conditions. Based on the growth/inhibition of the microbial cultures, the MIC at various concentrations was determined. The MICs determined was used for calculating the synergistic/indifferent/ antagonistic effects. 10 MIC of the combination was compared with the MIC of the actives alone and the fractional inhibitory concentration values (FIC index) was determined as follows:
FIC of Lavindin oil = MIC of lavindin oil in combination with vanillin
MIC of lavindin oil alone
FIC of Vanillin = MIC of vanillin in combination with lavindin oil
15 MIC of vanillin alone
FIC index = FIC of lavindin oil + FIC of vanillin The FIC index was interpreted to infer the effect of various combinations of active:
• A concentration of combination of actives is inferred as synergistic if FIC
index < 0.5
20 • A concentration of combination of actives is inferred as indifferent if 2 >
FIC index > 0.5
• A concentration of combination of actives is inferred as antagonistic if FIC
index > 2
25 [0074] The effect of various combinations of lavindin oil and vanillin against E. coli is herewith presented below in Table 2.

[0076] The varying concentrations of lavindin oil and vanillin may be noted from
the values listed under MIC (L:V) and MIC (V:L), respectively. From a combined
reading of Table 2 and Table 3, it can be inferred that lavindin oil at a
5 concentration range of 0.002 – 0.15 wt.% in combination with vanillin at a

concentration range of 0.004 – 0.16 wt.% was found to exhibit synergistic
antimicrobial efficacy. Furthermore, it may be noted the minimum inhibitory
concentration of each of the actives present in the combination varies as per the
micro-organism acted upon. This is evident from a working synergistic example
5 reproduced from the Tables 2 and 3, in the Table 4 below.
Formulation comprising of Lavindin oil and Vanillin
[0077] A formulation comprising of lavindin oil and vanillin, suitable carriers,
10 diluents, and excipients was further prepared for inhibition of bacterial growth as
detailed below in Table 5.

[0078] The formulation as seen above in Table 5 was prepared by a process
comprising the steps of a) mixing deionized water and glycerine in a first vessel to
obtain a first mixture at 27ºC; (b) adding acrylates/C10-30 alkyl acrylate cross-
5 polymer to the first mixture and mixed under vigorous agitation to obtain a
uniformly dispersed second mixture; (c) adding lavindin oil and vanillin to the
second mixture and mixed until a uniform third mixture is obtained; (d) adding a
pH stabilizer, triethanolamine, to the third mixture to neutralize the pH to 6.5-7;
(e) Mixing mineral oil, cetyl alcohol, glycol distearate, dimethicone, and allantoin
10 in a second vessel to obtain an uniform primary mixture; (f) mixing the primary
mixture with the mixture from (e) under rapid mixing conditions till a uniform
mixture is obtained; (g) adding phenoxyethanol, and perfume to the uniform
mixture of step in a serial manner to obtain said formulation.
Negative data
15 Example 1
[0079] It was observed that if vanillin is replaced with other known antimicrobial agents, synergistic antimicrobial effect may or may not be achieved. For example, it was observed that a combination of Dehydro-acetic acid (D) and Lavindin oil (L) does not provide a synergistic antimicrobial activity. The results are herewith
20 presented in Table 6.
Table 6

Negative data 2 Example 2
[0080] It is observed that if lavindin oil is replaced with other known antimicrobial
5 agents, synergistic antimicrobial effect may or may not be achieved. For example,
it was observed that a combination of Vanillin (V) and tea tree oil (TTO) does not
provide a synergistic antimicrobial activity. The results are herewith presented in
Table 7. Hence, from Tables 6 and 7 it may be noted that the choice of essential oil
(lavindin) and the herbal additive (vanillin) was non-obvious. Furthermore, it may
10 be appreciated that the working weight percentage ranges mentioned herein,
provided surprising synergistic ability against pathogenic bacteria.
Advantages of the present disclosure:
15 [0081] The present disclosure reveals a composition comprising herbal
ingredients that is effective against gram-positive and gram-negative bacteria, particularly against S. aureus and E. coli. The composition comprising lavindin oil to vanillin in the weight ratio is the range of 1:0.026 - 1:80 was found to synergistically inhibit said bacteria. The combination was found to be more
20 effective than commercially available synthetic biocides. The composition can be
easily adapted to obtain useful commercial compositions such as powders.

I/We Claim:
1. An antibacterial composition comprising:
a) lavindin oil; and
b) vanillin,
5 wherein lavindin oil to vanillin weight ratio is the range of 1:0.026 – 1:80.
2. The anti-bacterial composition as claimed in claim 1, wherein lavindin oil is
having weight percentage in range of 0.002 – 0.15 % with respect to the
composition.
3. The anti-bacterial composition as claimed in claim 1, wherein vanillin is
10 having weight percentage in range of 0.004 – 0.16 % with respect to the
composition.
4. A formulation comprising:
a) the composition as claimed in any one of the claims 1-3; and
b) at least one cosmetically suitable carrier.
15 5. The formulation as claimed in claim 4, wherein the at least one cosmetically
suitable carrier comprises additives selected from the group consisting of diluent, anti-caking agent, absorbent, skin protectant, viscosity modifier, opacifying agent, preservative, skin conditioning agent, cooling agent, odour enhancer, hydrophilic polymer, UV stabilizer, pH adjusting agent,
20 chelating agent, deodorant, perfumes, antimicrobial, antioxidant,
humectant, conditioning ingredients, propellants, salts, colorants, dyes, and combinations thereof. 6. The anti-bacterial composition as claimed in any one of the claims 1-3, wherein said composition inhibits growth of Gram-negative bacteria.
25 7. The anti-bacterial composition as claimed in any one of the claims 1-3,
wherein said composition inhibits growth of Gram-positive bacteria. 8. A process for preparing the composition as claimed in claim 1, said process comprising the steps of: a) obtaining lavindin oil; b) obtaining vanillin; and c) contacting lavindin oil with vanillin to obtain the composition.
30 9. A process for preparing the formulation as claimed in claim 4, said process
comprising the steps of: a) obtaining lavindin oil; b) obtaining vanillin; and

c) contacting lavindin oil and vanillin with at least one cosmetically suitable carrier to obtain the formulation. 10. The formulation as claimed in any one of the claims 4-5, is dispensed in a form selected from powders, sprays, emulsion, solution, antiseptics, patches, face washes, body washes, hand washes, pastes, liquid cleansing product, solid cleansing products, cleansing bars, and films.