DESC:AN EX-VIVO ANIMAL SURROGATE MODEL FOR STUDYING HUMAN HAIR DEVELOPMENT ,CLAIMS:1. A method for ex-vivo growth of goat skin hair follicles for study of human hair development, wherein the method comprises of:
a. isolating goat skin sections with hair follicles,
b. antibiotic and antimycotic treatment of isolated goat skin sections with hair follicles,
c. digestion of isolated goat skin sections with hair follicles with collagenase,
d. isolating intact goat skin hair follicles from goat skin sections, and
e. culturing of isolated goat skin hair follicles in a culture medium
2. The method as claimed in claim 1, wherein the goat skin sections with hair follicles is isolated from a group of animals that are in the age-range of 6 – 8 months, preferably 7 months.
3. The method as claimed in claim 1, wherein the goat skin sections with hair follicles is isolated from the group of body parts consisting of back side and tail, preferably tail.
4. The method as claimed in claim 1, wherein the isolated goat skin sections with hair follicles is treated with antibiotics and antimycotics selected from the group consisting of aminoglycosidal, bacteriostatic antimicrobial, broad spectrum polyketide antibiotic , amphotericin B, and polyene antifungal, preferably amphotericin B, aminoglycosidal, broad spectrum polyketide antibiotic, and bacteriostatic antibacterial.
5. The method as claimed in claim 4, wherein the antibiotic and antimycotic treatment is for a time period is 60 – 90 minutes, preferably 60 minutes.
6. The method as claimed in claim 4, wherein the concentration of the antibiotic and antimycotic is in the range of 30X – 50X, preferably 50X.
7. The method as claimed in claim 1, wherein concentration of collagenase is in the range of 3%-6%, preferably 3%.
8. The method as claimed in claim 1, wherein the isolated goat skin sections with hair follicles is digested with collagenase for a time period ranging from 3-4 hours, preferably 4 hours.
9. The method as claimed in claim 1, wherein the goat skin sections with hair follicles is digested at 370C.
10. The method as claimed in claim 1, wherein the goat skin sections with hair follicles is digested under CO2 concentration ranging from 4%-6%, preferably 5%.
11. A culture medium comprising of basal medium, antibiotic/antimycotic solution, gentamycine, chloramphenicol, tetracycline, kanamycin, amikacin, hydrocortisone, insulin and insulin transferrin selenium.
12. The culture medium as claimed in claim 11, wherein the basal medium is William’s E medium.
13. The culture medium as claimed in claim 11, wherein the concentration of the antibiotic/antimycotic solution is in the range of 1X-2X, preferably 2X.
14. The culture medium as claimed in claim11, wherein the antimycotic in the antibiotic/antimycotic solution is amphoterecin B.
15. The culture medium as claimed in claim 11, wherein the concentration of gentamycin is in the range of 100µg/mL-200µg/mL, preferably 100µg/ml.
16. The culture medium as claimed in claim 11, wherein the concentration of chloramphenicol is in the range of 50µg/mL-100µg/mL, preferably 100µg/mL.
17. The culture medium as claimed in claim 11, wherein the concentration of tetracycline is in the range of 25µg/mL-100µg/mL, preferably 25µg/mL.
18. The culture medium as claimed in claim 11, wherein the concentration of kanamycin is in the range of 50µg/mL-100µg/mL, preferably 50µg/mL.
19. The culture medium as claimed in claim 11, wherein the concentration of amikacin is in the range of 50µg/mL-100µg/mL, preferably 50µg/mL.
20. The culture medium as claimed in claim 11, wherein the concentration of hydrocortisone is in the range of 50µg/mL-100µg/mL, preferably 50µg/mL.
21. The culture medium as claimed in claim 11, wherein concentration of insulin is in the range of 50µg/mL100µg/mL, preferably 100µg/mL.
22. The culture medium as claimed in claim11, wherein the concentration of insulin transferrin selenium is in the range of 1X-3X, preferably 1X.
23. The culture medium as claimed in claim 11, wherein the pH of the culture medium is in the range of 7.4-7.6, preferably 7.4.
24. A method for ascertaining if a test compound is capable of modulating hair follicle activity, comprising contacting said test compound with a hair follicle grown in the culture medium as claimed in claim 11, measuring hair follicle activity parameters, and comparing said parameters to results from hair follicle grown said culture medium without contacting with said test compound.