DESC:FIELD

The present disclosure relates to an insecticidal composition and a process for preparation thereof.

DEFINITIONS

As used in the present disclosure, the following terms are generally intended to have the meaning as set forth below, except to the extent that the context in which they are used indicate otherwise.

Insecticide: An insecticide is a substance or a mixture of substances intended for preventing, destroying or controlling any insect, including vectors of human or animal disease, or animals causing harm or interfering with the production, processing, storage, transport or marketing of food, agricultural commodities, wood and wood products or animal feedstuffs, or substances which may be administered to animals for the control of insects, arachnids or other pests in or on their bodies.

Organophosphates: Organophosphates are foliar insecticides used primarily for the control of aphids, including resistant species, in vegetables and in horticulture.

Avermectin: Avermectins are a class of bio-insecticides that block the transmission of electrical activity in invertebrate nerve and muscle cells mostly by enhancing the effects of glutamate at the invertebrate-specific glutamate-gated chloride channel, with minor effects on gamma-aminobutyric acid receptors.

Neonicotinoid: Neonicotinoids are a class of neuro-active insecticides that disrupts an insect's nervous system by inhibiting nicotinic acetylcholine receptors.

Tank-Mix: Two or more insecticides or two or more insecticidal formulations mixed together in the spray tank at the time of application.

BACKGROUND

Insecticides are substances that are formulated to kill, harm, repel or mitigate one or more species of insects. Insecticides have different mode of actions, such as disrupting the nervous system of insects, damaging their exoskeletons or repelling them. Compositions comprising a combination of various insecticides are known for the management of different insects/pests. However, extensive use of insecticides can result in development of resistance in insects, and thereby decrease the crop yield. Further, the insecticides used in the combination may not be compatible with each other leading to rapid degradation of such compositions.

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Thus, there exists a need for insecticidal compositions that mitigates the hereinabove mentioned drawbacks.

OBJECTS

Some of the objects of the present disclosure, which at least one embodiment herein satisfies, are as follows:

It is an object of the present disclosure to ameliorate one or more problems of the prior art or to at least provide a useful alternative.

Another object of the present disclosure is to provide an insecticidal composition.

Still another object of the present invention is to provide a stable and ready to use insecticidal composition having a synergistic effect.

Yet another object of the present disclosure is to provide a process for preparing an insecticidal composition.

Yet another object of the present invention is to provide a method of treatment for plants.

Yet another object of the present disclosure is to provide a tank-mix to be constituted at the time of spraying.

Yet another object of the present invention is to provide an insecticidal kit.

Other objects and advantages of the present disclosure will be more apparent from the following description, which is not intended to limit the scope of the present disclosure.

SUMMARY

The present disclosure relates to an insecticidal composition and process for preparation thereof.

The insecticidal composition of the present disclosure comprises a neonicotinoid compound, an organophosphate compound, an avermectin compound, and at least two agrochemically acceptable excipients. The insecticides have different modes of action, and therefore exhibit enhanced efficacy when used in combination as compared to individual use.

In accordance with an aspect of the present disclosure there is provided a synergistic insecticidal composition comprising a neonicotinoid compound, an organophosphate compound, an avermectin compound and at least two agrochemically acceptable excipients in pre-determined proportion with respect to each other.

It is known that the avermectin compound is stable in alkaline condition, the organophosphate compound is stable in acid condition, and the neonicotinoid compound is stable in neutral condition. Hence, the avermectin compound and the organophosphate compound are incompatible with each other, and previous attempts to prepare a synergistic composition comprising these three active ingredients have been unsuccessful.

However, in the present disclosure, it is surprisingly found that a combination of agrochemically acceptable excipients in a pre-determined ratio aids in preventing the degradation of the active ingredients in the present synergistic composition.

In accordance with an embodiment of the present disclosure, the agrochemically acceptable excipients are selected from the group consisting of dispersing agent, wetting agent, binder, stabilizing agent, defoamer, polymeric film forming agent, and filler.

In accordance with another aspect of the present disclosure, there is provided a process for preparation of a synergistic insecticidal composition. The process comprises blending pre-determined quantities of an organophosphate compound, a neonicotinoid compound and an agrochemically acceptable excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent, together to obtain a mixture; blending pre-determined quantities of an avermectin compound, at least one stabilizing agent and a filler to obtain a blend; mixing the mixture and the blend to obtain a homogenized mixture; pounding the homogenized mixture to a fine particle size in the range of 1 µm to 10 µm to obtain a substantially homogenized powder; and converting the substantially homogenized powder into pre-determined dosage form and a tank-mix.

In accordance with yet another aspect of the present disclosure there is provided a method of controlling and eliminating insects from a pre-determined area by applying the synergistic insecticidal composition to the pre-determined area.

In accordance with a yet another aspect of the present disclosure, there is provided the synergistic insecticidal composition as a tank-mix and in the form of an insecticidal kit.

DETAILED DESCRIPTION

Embodiments, of the present disclosure, will now be described herein. Embodiments are provided so as to thoroughly and fully convey the scope of the present disclosure to the person skilled in the art. Numerous details are set forth, relating to specific components, and methods, to provide a complete understanding of embodiments of the present disclosure. It will be apparent to the person skilled in the art that the details provided in the embodiments should not be construed to limit the scope of the present disclosure. In some embodiments, well-known processes, well-known apparatus structures, and well-known techniques are not described in detail.

The terminology used, in the present disclosure, is only for the purpose of explaining a particular embodiment and such terminology shall not be considered to limit the scope of the present disclosure. As used in the present disclosure, the forms "a,” "an," and "the" may be intended to include the plural forms as well, unless the context clearly suggests otherwise. The terms "comprises," "comprising," “including,” and “having,” are open ended transitional phrases and therefore specify the presence of stated features, integers, steps, operations, elements, modules, units and/or components, but do not forbid the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. The particular order of steps disclosed in the method and process of the present disclosure is not to be construed as necessarily requiring their performance as described or illustrated. It is also to be understood that additional or alternative steps may be employed.

As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed elements.

The terms first, second, third, etc., should not be construed to limit the scope of the present disclosure as the aforementioned terms may be only used to distinguish one element, component, region, layer or section from another component, region, layer or section. Terms such as first, second, third etc., when used herein do not imply a specific sequence or order unless clearly suggested by the present disclosure.

The present disclosure relates to an insecticidal composition and a process for preparation thereof.

Dinotefuran [CAS No. 165252-70-0], also known as (RS)-1-methyl-2-nitro-3-(tetrahydro-3-furylmethyl) guanidine is an insecticide of the neonicotinoid class and sub class furanicotinyl, and its mechanism of action involves disruption of the insect's nervous system by inhibiting nicotinic acetylcholine receptors.

Dinotefuran

Imidacloprid [CAS No. 138261-41-3], also known as N-{1-[(6-Chloro-3-pyridyl) methyl]-4, 5-dihydroimidazol-2-yl} nitramide is an insecticide of the neonicotinoid class and causes blockage of the nicotinergic neuronal pathway. By blocking nicotinic acetylcholine receptors, imidacloprid prevents acetylcholine from transmitting impulses between nerves, resulting in the insect’s paralysis and eventual death.

Imidacloprid

Acetamiprid [CAS No. 135410-20-7], also known as N-[(6-chloro-3-pyridyl) methyl]-N'-cyano-N-methyl-acetamidine is an odorless neonicotinoid insecticide, mainly intended to control sucking insects. Acetamiprid is a nicotinic agonist that reacts with nicotinic acetylcholine receptors. The activation of the nicotinic acetylcholine receptors causes hyperactivity and muscle spasms, and eventually death.

Acetamiprid

Thiamethoxam [CAS No. 153719-23-4], also known as 3-[(2-Chloro-1, 3-thiazol-5-yl) methyl]-5-methyl-N-nitro-1, 3, 5-oxadiazinan-4-imine is a systemic insecticide in the class of neonicotinoids. It has a broad spectrum of activity against many types of insects. The compound gets in the way of information transfer between nerve cells by interfering with nicotinic acetylcholine receptors in the central nervous system, and eventually paralyzes the muscles of the insects.

Thiamethoxam

Acephate [CAS No. 30560-19-1], also known as N-(Methoxy-methylsulfanylphosphoryl) acetamide is an organophosphate foliar insecticide. It is used generally for the control of aphids. Acephate is converted to methamidophos in soil, plants, and insects. Acephate is a systemic insecticide that controls sucking and biting insects by direct contact or ingestion. It is capable of controlling leaf miners, caterpillars, sawflies and thrips in the crops as well as turf, and forestry.

Acephate

Quinalphos [CAS No. 13593-03-8], also known as O, O-Diethyl O-2-quinoxalinyl phosphorothioate. It is an organothiophosphate chemical chiefly used as a pesticide. It has a role as an acetylcholinesterase inhibitor, an acaricide and an agrochemical.

Quinalphos

Profenofos [CAS No. 41198-08-7], also known as 4-bromo-2-chloro-1-[ethoxy (propylsulfanyl) phosphoryl] oxybenzene, is an organophosphate pesticide primarily used against Lepidopteran insects.

Profenofos

Emamectin benzoate [CAS No. 155569-91-8], also known as 4''-Deoxy-4''-epi-methylamino-avermectin B1; Epi-methylamino-4''-deoxy-avermectin. It is a semi-synthetic derivative of the natural product abamectin in the avermectin family of 16-membered macrocyclic lactones. Avermectins are a family of 16-membered macrocyclic lactone natural product homologues produced by the soil microorganisms, Streptomyces avermitilis. Emamectin benzoate acts by the activating the chloride channels in insects.

Emamectin benzoate

It is known that the avermectin compound is stable in alkaline condition, the organophosphate compound is stable in acid condition, and the neonicotinoid compound is stable in neutral condition. Hence, the avermectin compound and the organophosphate compound are incompatible with each other, and previous attempts to prepare a synergistic composition comprising these three active ingredients have been unsuccessful.

However, in the present disclosure, it is surprisingly found that a combination of agrochemically acceptable excipients in a pre-determined ratio aids in preventing the degradation of the active ingredients in the present synergistic composition.

The insecticidal composition of the present disclosure comprises a neonicotinoid compound, an organophosphate compound, an avermectin compound, and at least two agrochemically acceptable excipients. The insecticides have different modes of action, and therefore exhibit enhanced efficacy when used in combination as compared to individual use.

In accordance with a first aspect of the present disclosure there is provided a synergistic insecticidal composition comprising a neonicotinoid compound, an organophosphate compound, an avermectin compound and at least two agrochemically acceptable excipients in pre-determined proportion with respect to each other.

In accordance with an embodiment of the present disclosure, the neonicotinoid compound is present in an amount in the range of 1% to 80% of the total mass of the composition, preferably from 1% to 50% of the total mass of the composition.

In accordance with an embodiment of the present disclosure, the neonicotinoid compound is selected from the group consisting of acetamiprid, clothianidin, imidacloprid, nitenpyram, dinotefuran, thiacloprid and thiamethoxam.

In an embodiment of the present disclosure, the neonicotinoid compound can be selected from dinotefuran, thiamethoxam, imidacloprid and acetamiprid.

In an embodiment of the present disclosure, the neonicotinoid compound is dinotefuran.

In accordance with an embodiment of the present disclosure, the organophosphate compound is present in an amount in the range of 1% to 90% of the total mass of the composition, preferably from 30% to 75% of the total mass of the composition.

In accordance with an embodiment of the present disclosure, the organophosphate compound is selected from the group consisting of acephate, demeton-S-methyl, dimethoate, parathion-methyl, phenoate, phorate, profenofos, quinalphos, triazophos, fenitrothion, fenthion, monocrotophos and oxydemeton-methyl.

In an embodiment of the present disclosure, the organophosphate can be selected from profenofos, quinalphos and acephate.

In an embodiment of the present disclosure, the organophosphate is acephate.

In accordance with an embodiment of the present disclosure, the avermectin compound is present in an amount in the range of 0.1% to 10% of the total mass of the composition, preferably from 0.5% to 7% of the total mass of the composition.

In accordance with an embodiment of the present disclosure, the avermectin compound is selected from the group consisting of ivermectin, selamectin, doramectin, emamectin, emamectin benzoate and abamectin.

In an embodiment of the present disclosure, the avermectin can be selected from emamectin, emamectin benzoate and abamectin.

In an embodiment of the present disclosure, the avermectin is emamectin benzoate.

In accordance with an embodiment of the present disclosure, the agrochemically acceptable excipients are selected from the group consisting of dispersing agent, wetting agent, binder, stabilizing agent, defoamer, polymeric film forming agent and filler.

In accordance with an embodiment of the present disclosure, the agrochemically acceptable excipients comprise a first excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent.

In accordance with an embodiment of the present disclosure, the agrochemically acceptable excipients comprise a second excipient comprising at least one stabilizing agent and a filler.

In accordance with an embodiment of the present disclosure, the second excipient comprises at least two stabilizing agents and a filler.

In accordance with an embodiment of the present disclosure, the agrochemically acceptable excipients comprise a first excipient and a second excipient.

In accordance with an embodiment of the present disclosure, the agrochemically acceptable excipients are present in an amount in the range of 2.1% to 75% of the total mass of the composition, preferably from 3% to 50% of the total mass of the composition.

Typically, the amount of the dispersing agent can be in the range of 0.2 % to 20 % of the total mass of the composition, the amount of the wetting agent can be in the range of 0.1 % to 10% of the total mass of the composition, the amount of the binder can be in the range of 0.5% to 10% of the total mass of the composition, the amount of the stabilizing agent can be in the range of 0.2% to 5% of the total mass of the composition, the amount of the filler can be in the range of 1% to 30% % of the total mass of the composition, the amount of the polymeric film forming agent can be in the range of 0.5 % to 10% of the total mass of the composition and the amount of the defoamer can be in the range of 0.1% to 3% of the total mass of the composition.

In accordance with an embodiment of the present disclosure, the wetting agent is at least one selected from the group consisting of salts of aliphatic monoesters of sulphuric acid including sodium lauryl sulphate, sulfoalkylamides and salts thereof , N - methyl - N - oleoyltaurate Na salt, alkylarylsulfonates, alkylbenzene sulfonates, alkylnaphthalene sulfonates and salts thereof and salts of ligninsulfonic acid,aryl sulfonates (such as sodium dodecylbenzene sulfonate, sodium alkylnaphthalene sulfonate) or combinations thereof.

In accordance with an embodiment of the present disclosure, the dispersing agent is at least one selected from the group salts of naphthalenesulphonic acid / formaldehyde condensates , salts of polystyrene sulphonic acids , poly carboxylates polyethylene glycol ethers of linear alcohols, salts of polyvinylsulphonic acids, salts of condensates of naphthalenesulphonic acid, salts of lignosulphonic acid, EO/PO block copolymers, styrene acrylic copolymer furthermore alkyl ethoxylates and alkylarylethoxylates, ethoxylated alkylarylphosphated and sulphated ester or combinations thereof.

In accordance with an embodiment of the present disclosure, the binder or film forming agent can be at least one selected from the group, consisting of polyvinyl alcohol, polyvinylpyrrolidone, Starch, Dextrin, or combinations thereof.

In accordance with an embodiment of the present disclosure, the defoamer can be Silicon base antifoam.

In accordance with an embodiment of the present disclosure, the stabilizing agent is at least one selected from the group, consisting of phosphoric acid monoesters, phosphoric acid diesters, phosphoric acid mono- and di- ester mixture, sodium phosphates, butylated hydroxytoluene, castor oil ethoxylate, ethoxylated hydrogenated castor oil, vegetable oil and epoxidized soyabean oil or combinations thereof.

In a first exemplary embodiment of the present disclosure, the stabilizing agent is selected from phosphoric acid monoesters or diesters or mono and di ester mixture and sodium phosphates in a pre-determined ratio.

Phosphoric Acid Mono- and Di- Ester mixture are also known as Isopropyl dihydrogen phosphate; Monoisopropyl phosphate; Phosphorylisopropane; Isopropyl phosphoric acid; Isopropyl acid phosphate.

Sodium phosphates are also known as Disodium hydrogen phosphate; Disodium phosphate, Disodium hydrogenorthophosphate; Sodium phosphate dibasic; Dibasic sodium phosphate

In accordance with an embodiment of the present disclosure, the film-forming agent is at least one selected from the group consisting of hydrophilic polymers, hydrobhobic polymers, Poly(vinylpyrrolidone), vinypyrrolidone-vinylacetate and the filler, at least one selected from the group consisting of lactose, glucose, fructose, mannose, mannitol, sucrose, and the like, can also act as binding agent..

In a second exemplary embodiment, the present disclosure provides a synergistic insecticidal composition comprising the neonicotinoid compound in an amount in the range of 1% to 80% of the total mass of the composition, organophosphate compound in an amount in the range of 1% to 90% of the total mass of the composition, avermectin compound in an amount in the range of 0.1% to 10% of the total mass of the composition and at least two agrochemically acceptable excipients in an amount in the range of 0.5% to 75% of the total mass of the composition.

In a third exemplary embodiment, the present disclosure provides a synergistic insecticidal composition comprising the neonicotinoid compound in an amount in the range of 1% to 80% of the total mass of the composition, organophosphate compound in an amount in the range of 1% to 90% of the total mass of the composition, avermectin compound in an amount in the range of 0.1% to 10% of the total mass of the composition, a first excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent and a second excipient comprising stabilizing agent selected from phosphoric acid mono and di ester mixture and sodium phosphates in an amount in the range of 0.2% to 5% of the total mass of the composition and a filler in an amount in the range of 1% to 30% of the total mass of the composition.

In an embodiment, the present disclosure provides a synergistic insecticidal composition comprising the neonicotinoid compound in an amount in the range of 1% to 80% of the total mass of the composition, organophosphate compound in an amount in the range of 1% to 90% of the total mass of the composition, avermectin compound in an amount in the range of 0.1% to 10% of the total mass of the composition, a first excipient is polymeric film-forming agent and a second excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and a filler in an amount in the range of 1% to 30% of the total mass of the composition and stabilizing agent selected from phosphoric acid mono and di ester mixture and sodium phosphates in an amount in the range of 0.2% to 5% of the total mass of the composition.

In an embodiment, the present disclosure provides a synergistic insecticidal composition of Dinotefuran, in an amount in the range of 1% to 50% of the total mass of the composition, Acephate in an amount in the range of 30% to 75% of the total mass of the composition, Emamectin benzoate in an amount in the range of 0.5% to 7% of the total mass of the composition and at least one agrochemically acceptable excipient in an amount in the range of 0.5% to 50% of the total mass of the composition.

In an embodiment, the present disclosure provides a synergistic insecticidal composition of Dinotefuran, in an amount in the range of 1% to 50% of the total mass of the composition, Acephate in an amount in the range of 30% to 75% of the total mass of the composition, Emamectin benzoate in an amount in the range of 0.5% to 7% of the total mass of the composition, the polymeric film forming agent in an amount in the range of 0.1 % to 10% of the total mass of the composition, at least one stabilizing agent in an amount in the range of 0.2% to 5% of the total mass of the composition and the filler in an amount in the range of 1% to 30% % of the total mass of the composition.

In accordance with a second aspect of the present disclosure, the composition can be in at least one dosage form selected from the group consisting of soluble granules, soluble powder, soluble concentrate (SL), an emulsifiable concentrate (EC), an emulsion (EW) , a micro-emulsion (ME), an oil-based suspension concentrate (OD), a flowable suspension (FS), a water-dispersible granule (WG), a water-dispersible powder (WP), a granule (GR), an encapsulated granule (CG), a fine granule (FG) , a macrogranule (GG), an aqueous suspo-emulsion (SE), a microencapsulated suspension (CS) , a microgranule (MG) or a suspension concentrate (SC).

In an embodiment of the present disclosure, the insecticidal composition is in the form of soluble granules having a particle size in the range of 0.5 mm to 2 mm.

In an embodiment of the present disclosure, the insecticidal composition is a tank-mix to be constituted at the time of application.

In an embodiment of the present disclosure, the constituents of the combination of the present invention may be tank mixed and sprayed at the locus of the infection, or alternatively may be mixed with surfactants and then sprayed.

In accordance with a third aspect of the present disclosure, there is provided a process for preparing an insecticidal composition. The process involves the following steps:

a. blending pre-determined quantities of an organophosphate compound, a neonicotinoid compound and an agrochemically acceptable first excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent, together to obtain a first substantially homogenized mixture;
b. blending pre-determined quantities of an avermectin compound, at least one stabilizing agent and a filler separately to obtain a second substantially homogenized mixture;
c. mixing the first substantially homogenized mixture with the second substantially homogenized mixture to obtain a third homogenized mixture;
d. pounding the third homogenized mixture to a fine particle size in the range of 1 µm to 10 µm to obtain a substantially homogenized powder; and
e. converting the substantially homogenized powder into one dosage form selected from the group consisting of soluble granules, water dispersible granules, suspension concentrate, emulsifiable concentrate soluble powder and a tank-mix.

In accordance with an embodiment of the present disclosure, the third homogenized mixture is pounded in an air jet mill to obtain the substantially homogenized powder.

In accordance with an embodiment of the present disclosure, the particle size of the substantially homogenized powder is in the range of 1 µm to 10 µm.

In accordance with an embodiment of the present disclosure, the substantially homogenized powder is further processed as follows:
a. the substantially homogenized powder and water are added to a dough maker and kneaded into a dough;
b. the dough is processed in an extruder to obtain wet extruded granules; and
c. the wet extruded granules are dried under controlled drying conditions to obtain dried extruded granules.

Typically, the wet extruded granules are dried at a temperature in the range of 30 °C to 70 °C and the granule size of the dried extruded granules is in the range of 0.5 mm to 2 mm.

In accordance with a fourth aspect of the present disclosure, there is provided a method of controlling and eliminating insects from a pre-determined area, said method comprising applying to a pest, to a locus of a pest or to a plant susceptible to attack by a pest, an effective amount of the synergistic insecticidal composition of the present disclosure.

In accordance with an embodiment of the present disclosure, the synergistic insecticidal composition is used against pests selected from the group consisting of sucking pests (Bemisia tabaci and Amrasca devastans) and Lepidopteran pests (Helicoverpa armigera and Spodoptera litura), Chewing pests, Biting pests, Boring pests and Mites.

In an embodiment of the present disclosure, the insecticidal composition may be used for foliar application, ground application or applications to plant propagation materials. The application may be made to the soil before emergence of the plants, either pre-planting or post-planting. The application may be made as a foliar spray at different timings during crop development, with either one or two applications early or late post-emergence.

The combinations of the present invention may be sold as a pre-mix composition or a kit of parts such that individual actives may be mixed before spraying.

In accordance with the present disclosure there is provided a kit comprising an insecticidal combination comprising neonicotinoid; organophosphate or a combination thereof; and at least an avermectin compound.

In accordance with a fifth aspect of the present disclosure, there is provided an insecticidal kit comprising,
a. a first sachet containing a first substantially homogenized mixture comprising predetermined quantities of an organophosphate compound, a neonicotinoid compound, and an agrochemically acceptable first excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent;
b. a second sachet containing a second substantially homogenized mixture comprising pre-determined quantities of an avermectin compound, at least one stabilizing agent and filler; and
c. an instruction pamphlet;
wherein the contents of the first sachet and the second sachet are to be mixed in accordance with the instructions provided in the instruction pamphlet.

The present disclosure provides a synergistic insecticidal composition comprising three active ingredients, namely, Dinotefuran, Acephate and Emamectin benzoate formulated as a stable composition using at least two agrochemically acceptable excipients.

EXAMPLES

Example 1: Acephate 75% + Dinotefuran 5% + Emamectin Benzoate 1.25 % SG
78g Acephate technical, 5.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.4g Emamectin benzoate technical and 13.3g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 2: Acephate 75% + Dinotefuran 5% + Emamectin Benzoate 1.25 % SG
78g Acephate technical, 5.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.4g Emamectin benzoate technical, 1g of stabilising agent A and 12.3g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3 ml of water are added to a dough maker and needed to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 3: Acephate 75% + Dinotefuran 5% + Emamectin Benzoate 1.25 % SG
78g Acephate technical, 5.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.4g Emamectin benzoate technical and 2g of stabilising agent B, 11.3g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3.8 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 4: Acephate 75% + Dinotefuran 5% + Emamectin Benzoate 1.25 % SG
78g Acephate technical, 5.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.4g Emamectin benzoate technical and 1g of stabilising agent A and 0.5g of stabilising agent B, 11.8g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3.4 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 5: Acephate 75% + Dinotefuran 5% + Emamectin Benzoate 1.25 % SG
78g Acephate technical, 5.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.4g Emamectin benzoate technical and 2g of stabilising agent C, 11.3g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3.4 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 6: Acephate 75% + Dinotefuran 5% + Emamectin Benzoate 1.25 % SG
78g Acephate technical, 5.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.4g Emamectin benzoate technical and 2g of stabilising agent D, 11.3g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3.4 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 7: Acephate 75% + Dinotefuran 5% + Emamectin Benzoate 1.25 % SG
78g Acephate technical, 5.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.4g Emamectin benzoate technical and 1g of stabilising agent E, 12.3g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 8: Acephate 75% + Dinotefuran 4% + Emamectin Benzoate 1 % SG
78g Acephate technical, 4.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.2g Emamectin benzoate technical and 1g of stabilising agent A and 0.5g of stabilising agent B, 13g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3.4 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 9: Acephate 70% + Dinotefuran 6% + Emamectin Benzoate 1.5 % SG
73g Acephate technical, 6.3g Dinotefuran Technical, 2g vinylpyrrolidone-vinylacetate is blended in a ribbon blender to obtain a substantially homogenized mixture A.
1.7g Emamectin benzoate technical and 0.5g of stabilising agent A and 1g of stabilising agent B, 15.5g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in a ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm.

Example 10: Acephate 55 % + Thiamethoxam 4.5% + Emamectin Benzoate 1% SG
Initially, 58g Acephate technical, 4.8g Thiamethoxam, 2g vinylpyrrolidone-vinylacetate blended in ribbon blender to obtain a substantially homogenized mixture A.
1.2 g Emamectin benzoate technical, 1g stabilizing agent ‘A’ and 0.5g stabilizing agent ‘B’and 32.5g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules. In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm

Example 11: Acephate 70% + Imidacloprid 3% + Emamectin Benzoate 1.4 % WG
Initially, 73g Acephate technical, 3.4g Imidacloprid, 2g vinylpyrrolidone-vinylacetate blended in ribbon blender to obtain a substantially homogenized mixture A.
1.6g Emamectin benzoate technical, 1g stabilizing agent ‘A’ and 0.5g stabilizing agent ‘B’and 18.5g lactose are blended separately to obtain homogenized mixture B.
Mixture A is further blended with Mixture B in ribbon blender to obtain Homogenized mixture C.
Homogenised mixture C is pounded to a fine particle size to obtain a substantially homogenized powder D
Typically, the particle size of the substantially homogenized powder D can be in the range of 1 µm to 10 µm.
In the embodiment, the mixture is pounded in an air jet mill.
Further pre-determined quantities of the substantially homogenized powder D and 3 ml of water are added to a dough maker and kneaded to obtain dough. The dough is then processed in an extruder to obtain wet extruded granules. The wet extruded granules are dried under controlled drying conditions temperature of 50 degree to obtain dried extruded granules.
In an embodiment, the dried extruded granules can be, further, sieved to obtain dried extruded granules having granule size in the range of 0.5 mm to 2 mm

Example 12: Quinalphos 40% + Acetamiprid 2.5 % + Emamectin Benzoate 1.4 % EC
Quinalphos 40% active content , Acetamiprid 2.5 %, Emamectin Benzoate 1.4 %, calcium dodecylbenzenesulfonate 2.25%, Castor oil ethoxylate 40 moles 12.75%, remaining xylene solvent to make up 100%, formulation the components were uniformly mixed and stirred to give 43.9% EC.

Example 13: Profenofos 50% + Dinotefuran 4% + Emamectin Benzoate 0.8 % EC
50% profenofos, Dinotefuran 4%, Emamectin Benzoate 0.8 % ,Caster oil poly gylcol ether 36/40 grade 12%, Dodecyl benzene sulfonic acid calcium salt 3%, remaining xylene solvent to make up 100%, formulation the components were uniformly mixed and stirred to give 54.8% EC.

EXPERIMENTS

1. ACCELERATED STORAGE STABILITY STUDY

The compositions formulated in the above Examples 1 to 7 are subjected to accelerated storage stability study at 54 °C for a period of 14 days. The results are shown in Table No. 1.

TABLE 1
Examples Stabilizing Agents Acephate A.I. % Dinotefuran A.I. % Emamectin Benzoate A.I. % PH1%
RT ASH % degradation RT ASH % degradation RT ASH % degradation RT ASH
1 Without stabilizing agents 75.52 71.00 5.985% 5.25 4.18 20.38% 1.26 0.77 38.89% 3.9 3.0
2 A 75.4 74.90 0.66% 5.21 5.15 1.15% 1.25 1.19 4.80% 3.3 3.5
3 B 75.6 71.8 5.03% 5.11 5.05 1.17% 1.27 1.10 8.33% 6.4 6.2
4 A+B 75.5 75.2 0.40% 5.15 5.12 0.58% 1.26 1.21 3.97% 3.4 3.3
5 C 75.00 74.00 1.33% 5.1 4.50 11.76% 1.27 1.02 19.68% 4.2 3.7
6 D 75.4 73 3.20% 5.12 4.85 5.27% 1.26 0.45 64.28% 4.7 3.8
7 E 75.5 73 3.30% 5.3 4.32 18.5% 1.22 0.68 42.51% 3.3 3.2

Stabilizing Agents: A=Isopropyl Phosphate, B= Disodium Hydrogen Phosphate, C=Butylated hydroxytoluene, D=Castor oil 40 ethoxylate, E= Epoxidised soyabean oil
RT=Room Temperature; ASH= Accelerator Storage Stability Study at 54 °C for 14 days

RESULTS:
Although the three active components have enhanced synergistic effect upon combination, they have a tendency to degrade under different pH conditions. Hence, there is a need to incorporate stabilizing agents to prevent degradation of the active ingredients on storage.
The above results (Table 1) clearly indicate that in the absence of stabilizing agent(s), (Example 1), the active ingredients of the present composition show degradation on storage. It is evident that use of a combination of isopropyl phosphate and disodium hydrogen phosphate (Example 4) as a stabilizing agent provides a stable synergistic insecticidal composition.

2. EVALUATION OF DINOTEFURAN + EMAMECTIN BENZOATE + ACEPHATE FOR BIO-EFFICACY AGAINST SUCKING PESTS ( Bemisia tabaci, Amrasca devastans) AND LEPIDOPTERAN PESTS ( Helicoverpa armigera, Spodoptera litura)
Objectives:
? To study efficacy of Dinotefuran + Emamectin benzoate + Acephate against Sucking and Lepidopteran pests
? To study comparative efficacy of test insecticides with market standards Dinotefuran 20% SG, Emamectin benzoate 5% SG and Acephate 75%WP and their tank-mix combinations.
Test Products:
• Dinotefuran 4% + Emamectin benzoate 1% + Acephate 75% SG
• Dinotefuran 5% + Emamectin benzoate 1.25% + Acephate 75% SG
• Dinotefuran 6% + Emamectin benzoate 1.50% + Acephate 70% SG
Test Crop: Abelmoschus esculentus; Variety/Cultivar: Okra, Arka Anamika
Number of Applications: Two applications at an interval of 15 days.
Time of Observation:
? Before 1st Spray
? 1, 7 and 15 days after 1st Spray
? 1, 7, 15 and 21 days after 2nd Spray
A ready formulation test insecticide of three way combinations of Dinotefuran, Emamectin Benzoate, Acephate were tested at given concentrations with tank-mix combination, i.e., Dinotefuran 20% SG + Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix), 3 Premix recipes of Dinotefuran, Emamectin Benzoate, Acephate (Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG, Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG, Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG) along with two way (Tank-Mix) & solo individual insecticides, i.e., Dinotefuran 20% SG + Emamectin Benzoate 5% SG (Tank-Mix), Dinotefuran 20% SG + Acephate 75% SP (Tank-Mix), Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix), Dinotefuran 20% SG, Emamectin Benzoate 5% SG, Acephate 75% SP and Untreated Control against Whitefly, Jassids, and Lepidopteran pests of Okra. The insecticides were applied as foliar spray with Knapsack Sprayer fitted with hollow cone nozzle. Application was initiated with build of pest population to Economical Threshold Level (ETL) in the field. The sprayings were done at 15 days interval.
One day prior of the initiation of the experiment, pest incidence was recorded, and subsequent observations were recorded after 7, and 15 days of each spray. The Whitefly and Jassids were counted on 5 pre-tagged plants per plot as 6 leaves selected randomly / plant (2 leaves each from top, middle and bottom). For Lepidopteran pests, the population per plant was counted on 5 pre-tagged plants per plot. Mean value were derived for the treatment and used for Statistical analysis of the data.

RESULTS:
a. Bio-efficacy: Whitefly (Bemisia tabaci)
The results presented (TABLE 2) shows that at the time of initiation of trial there was uniform population of Whitefly across all the treatments. At 7 days after first application, the highest Whitefly population was recorded in control (13.60/leaf). All the insecticide treatments significantly reduced the whitefly population than untreated control, but the significant lowest number of whitefly was observed in T2 (0.14/leaf), which was significantly superior over rest of all treatments. At 21 days after first application, the highest whitefly population was recorded in control (26.54/leaf). The significant lowest whitefly population was observed in T2 (2.0/leaf) which was statistically superior with rest of the treatments. Similar trend were observed on 15 days after first & second application with T2 (0.2/leaf & 1.0/leaf) and Untreated recorded T11 (19.67/leaf & 25.2/leaf) respectively. The treatment T2 recorded the least number of whitefly at 21 days after second spray (2.0/leaf), followed by T4 (2.47/leaf) and T3 (3.4/leaf). The highest number of whitefly was recorded in treatment T11 (26.54/leaf) which was untreated control.
TABLE 2
Bio efficacy of different insecticides treatment against Whitefly of Okra (summer - Kharif 2017)
Tr. No.
Treatment Details Dose
(g a.i./ha) Dose
( g/ha) Pre-count Mean Number of Whitefly/leaf
1 DAA 1st spray 7 DAA 1st spray 15 DAA 1st spray 1 DAA 2nd spray 7 DAA 2nd spray 15 DAA 2nd spray 21 DAA 2nd spray
T1 Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG 20+5+375 500 11.87 (3.51) 6.47 (2.62) 3.67 (2.04) 4.27 (2.19) 2.74 (1.8) 3 (1.87) 3.4 (1.97) 4.87 (2.31)
T2 Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG 25+6.25+375 500 15.07 (3.92) 4.54 (2.25) 0.14 (0.79) 0.2 (0.84) 0.00 (0.71) 0.00 (0.71) 1.00 (1.23) 2.00 (1.57)
T3 Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG 30+7.50+350 500 17.00 (4.18) 5.27 (2.41) 1.34 (1.35) 2.14 (1.63) 1.54 (1.43) 1.27 (1.33) 1.94 (1.56) 3.4 (1.97)
T4 Dinotefuran 20% SG + Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 30+8.5+585 150+170+780 12.07 (3.55) 4.94 (2.33) 0.34 (0.92) 0.47 (0.99) 0.20 (0.84) 0.20 (0.84) 1.34 (1.34) 2.47 (1.69)
T5 Dinotefuran 20% SG + Emamectin Benzoate 5% SG (Tank-Mix) 30+8.5 150+170 13.87 (3.79) 8.64 (3.03) 5.80 (2.51) 7.34 (2.8) 4.14 (2.15) 4.34 (2.2) 4.8 (2.31) 5.87 (2.53)
T6 Dinotefuran 20% SG + Acephate 75% SP (Tank-Mix) 30+585 150+780 16.67 (4.14) 8.27 (2.96) 5.54 (2.46) 6.40 (2.63) 3.20 (1.93) 3.67 (2.02) 4.47 (2.22) 5.77 (2.5)
T7 Emamectin Benzoate 5% SG + Acephate 75% SP (Tank -Mix) 8.5+585 170+780 13.47 (3.74) 10.20 (3.28) 11.47 (3.46) 14.00 (3.81) 8.87 (3.07) 12.6 (3.61) 14.27 (3.83) 15.6 (3.99)
T8 Dinotefuran 20% SG 30 150 17.47 (4.21) 8.67 (3.03) 9.40 (3.15) 10.74 (3.36) 5.67 (2.49) 12.00 (3.53) 12.94 (3.66) 14.27 (3.84)
T9 Emamectin Benzoate 5% SG 8.5 170 11.6 (3.47) 11.87 (3.51) 13.47 (3.74) 15.47 (3.99) 13.94 (3.8) 18.4 (4.34) 19.54 (4.47) 21.67 (4.71)
T10 Acephate 75% SP 585 780 13.47 (3.74) 10.84 (3.37) 13.74 (3.78) 16.34 (4.1) 13.60 (3.76) 16.14 (4.07) 17.54 (4.24) 19.74 (4.5)
T11 Control - - 12.34 (3.58) 12.47 (3.6) 13.60 (3.76) 19.67 (4.5) 19.80 (4.51) 23.14 (4.86) 25.2 (5.07) 26.54 (5.20)
CD (P=0.05) NS 0.32 0.20 0.26 0.19 0.5 0.51 0.52
DAA-Days after Application; NS-Non Significant

b. Bio-Efficacy: Jassids (Amrasca devastans)
The results presented (TABLE 3) shows that at the time of initiation of trial there was no significant difference between the treatments which indicates the uniform prevalence of the pest. At 7 days after first application, the highest number of Jassids was recorded in control (18.94/leaf). The significant lowest Jassids population was observed in T2 (0.60/leaf). The treatment T2 was significantly superior over rest of all the treatments. Similar trend was observed on 15 days after first application. At 15 days after first application also, the significantly less number of Jassids was observed in treatment T2 (1.20/leaf), which was superior over rest of all treatments. The highest Jassids were recorded in control (22.2/leaf). At 7 days after second application, there is good suppression of Jassids population in the treatments. Very less number of Jassids were observed in T2 (0.54/leaf) which is significantly lowest and the highest was in untreated control that is T11 (25.04/leaf). The similar trend observed on 15 days after second application. At 21 days after second application also similar trend observed. The significant lowest Jassids were observed in treatment T2 (1.94 /leaf), which was superior over rest of all treatments. The highest population of Jassids was observed in untreated control (27.67/leaf). The treatment (T2) was significantly superior over rest of all treatments.
TABLE 3
Bio-efficacy of different Insecticides treatments against Jassids of Okra (Summer-Kharif 2017)
Tr. No Treatment Details Dose
(g a.i./ha) Dose
(ml or g/ha) Pre-count Mean Number of Jassids/leaf
1 DAA 1st spray 7 DAA 1st spray 15 DAA 1st spray 1 DAA 2nd spray 7 DAA 2nd spray 15 DAA 2nd spray 21 DAA 2nd spray
T1 Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG 20+5+375 500 16.34 (4.11) 7.27 (2.79) 1.47 (1.4) 3.80 (2.08) 1.80 (1.52) 2.94 (1.86) 4.47 (2.23) 5.2 (2.39)
T2 Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG 25+6.25+375 500 15 (3.94) 4.27 (2.18) 0.60 (1.04) 1.20 (1.29) 0.34 (0.91) 0.54 (1.02) 1.40 (1.38) 1.94 (1.56)
T3 Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG 30+7.50+350 500 16.34 (4.04) 5.20 (2.39) 1.40 (1.38) 1.87 (1.54) 1.07 (1.25) 1.60 (1.45) 2.80 (1.82) 3.10 (1.9)
T4 Dinotefuran 20% SG + Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 30+8.5+585 150+170+780 16.34 (4.06) 4.47 (2.23) 1.00 (1.23) 1.47 (1.4) 0.54 (1.01) 0.74 (1.11) 1.67 (1.48) 2.14 (1.63)
T5 Dinotefuran 20% SG + Emamectin Benzoate 5% SG (Tank-Mix) 30+8.5 150+170 19.34 (4.44) 9.7 (3.18) 1.94 (1.56) 4.00 (2.12) 2.60 (1.76) 5.20 (2.39) 5.94 (2.54) 6.27 (2.61)
T6 Dinotefuran 20% SG + Acephate 75% SP (Tank-Mix) 30+585 150+780 16.14 (4.08) 9.54 (3.16) 1.80 (1.51) 3.87 (2.09) 2.00 (1.57) 4.87 (2.32) 5.67 (2.49) 6.00 (2.55)
T7 Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 8.5+585 170+780 16.87 (4.16) 14.07 (3.81) 2.94 (1.85) 5.87 (2.53) 4.87 (2.32) 7.87 (2.89) 9.07 (3.1) 10.47 (3.32)
T8 Dinotefuran 20% SG 30 150 16.94 (4.18) 9.00 (3.06) 2.14 (1.62) 4.80 (2.31) 2.20 (1.64) 5.27 (2.41) 6.00 (2.55) 6.20 (2.59)
T9 Emamectin Benzoate 5% SG 8.5 170 17.67 (4.25) 14.30 (3.85) 12.80 (3.65) 14.94 (3.93) 13.07 (3.68) 17.47 (4.24) 19.67 (4.5) 21.20 (4.66)
T10 Acephate 75% SP 585 780 17.34 (4.15) 12.4 (3.55) 9.47 (3.09) 12.4 (3.59) 10.67 (3.34) 13.87 (3.79) 17.77 (4.28) 19.20 (4.44)
T11 Control - - 13.67 (3.77) 14.4 (3.86) 18.94 (4.41) 22.2 (4.74) 22.8 (4.81) 25.04 (5.04) 26.07 (5.14) 27.67 (5.30)
CD (P=0.05) NS 0.52 0.47 0.41 0.45 0.35 0.26 0.26
DAA-Days after Application; NS-Non Significant

c) Bio-efficacy: Bhendi fruit borer (Helicoverpa armigera)
The results presented (TABLE 4) shows that at the time of initiation of trial, the thrips population was uniformly present across the plot. At 7 days after first application, the highest number of Bhendi fruit borer was recorded in untreated control (3.0/plant). The significant lowest Bhendi fruit borer was observed in T2 (0.00/plant). The treatment T2 was significantly superior over rest of all treatments. Similar pattern of observations were recorded on 15 days after first application. At 15 days after first application, there was significantly less number of fruit borer was observed in treatment T2. The highest fruit borer was recorded in control (3.54/plant). The significant lowest number of fruit borer was in T2 (0.14/plant), which was superior over rest of all treatments. At 7 days after second application, there was good suppression of fruit borer population in all the insecticide spray treatments. The treatment T2 recorded fruit borer infestation (0.20/plant), which is significantly lowest among all the treatments and the highest population of fruit borer was noticed in untreated control T11 (4.0/plant). Same kind of trend was recorded on 15 days after second application. At 21 days after second application also similar trend was observed. The significant lowest fruit borer was recorded in treatment T2 (1.14 /plant), which was superior over rest of all the treatments. The highest fruit borer was observed in untreated control (5.54/plant).
TABLE 4
Bio-efficacy of different Insecticides treatments against Helicoverpa armigera on Okra (Summer-Kharif 2017)
Tr. No Treatment Details Dose
(g a.i./ha) Dose
(ml or g/ha) Pre-count Mean Number of Helicoverpa armigera /plant
1 DAA 1st spray 7 DAA 1st spray 15 DAA 1st spray 1 DAA 2nd spray 7 DAA 2nd spray 15 DAA 2nd spray 21 DAA 2nd spray
T1 Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG 20+5+375 500 2.2 (1.64) 2.07 (1.58) 0.8 (1.13) 1.07 (1.24) 1.00 (1.22) 1.54 (1.42) 1.94 (1.56) 2.47 (1.72)
T2 Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG 25+6.25+375 500 2.2 (1.63) 1.27 (1.33) 0.00 (0.71) 0.14 (0.79) 0.00 (0.71) 0.2 (0.84) 0.47 (0.98) 1.14 (1.28)
T3 Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG 30+7.50+350 500 2.07 (1.61) 1.07 (1.26) 0.00 (0.71) 0.27 (0.87) 0.00 (0.71) 0.27 (0.88) 0.67 (1.08) 1.07 (1.26)
T4 Dinotefuran 20% SG + Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 30+8.5+585 150+170+780 1.67 (1.47) 1.40 (1.37) 0.07 (0.76) 0.34 (0.92) 0.07 (0.76) 0.27 (0.87) 0.54 (1.01) 1.47 (1.39)
T5 Dinotefuran 20% SG + Emamectin Benzoate 5% SG (Tank-Mix) 30+8.5 150+170 2.20 (1.63) 1.80 (1.51) 0.00 (0.71) 1.47 (1.4) 0.8 (1.14) 1.07 (1.25) 1.47 (1.4) 3.2 (1.88)
T6 Dinotefuran 20% SG + Acephate 75% SP (Tank-Mix) 30+585 150+780 2.54 (1.74) 2.07 (1.59) 1.40 (1.37) 1.94 (1.56) 1.87 (1.54) 2.40 (1.71) 2.74 (1.8) 3.54 (2.01)
T7 Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 8.5+585 170+780 2.34 (1.68) 2.20 (1.64) 0.34 (0.91) 1.30 (1.34) 0.40 (0.95) 0.87 (1.17) 1.14 (1.28) 1.74 (1.5)
T8 Dinotefuran 20% SG 30 150 2.47 (1.72) 2.47 (1.72) 1.87 (1.54) 2.67 (1.78) 2.47 (1.72) 3.00 (1.88) 3.40 (1.98) 4.07 (2.14)
T9 Emamectin Benzoate 5% SG 8.5 170 2.87 (1.84) 2.00 (1.58) 0.27 (0.87) 1.94 (1.56) 1.00 (1.22) 1.14 (1.28) 1.40 (1.38) 1.87 (1.54)
T10 Acephate 75% SP 585 780 2.34 (1.67) 2.20 (1.63) 2.07 (1.59) 2.74 (1.8) 2.34 (1.68) 2.94 (1.86) 3.20 (1.93) 4.27 (2.19)
T11 Control - - 2.14 (1.6) 2.20 (1.63) 3.00 (1.87) 3.54 (2.01) 3.60 (2.02) 4.00 (2.12) 4.4 (2.22) 5.54 (2.46)
CD (P=0.05) NS 0.4 0.27 0.28 0.22 0.21 0.22 0.32
DAA-Days after Application; NS-Non Significant

d) Bio-efficacy: Bhendi caterpillar (Spodoptera litura)
The results presented (TABLE 5) shows that, at the time of initiation of trial, the population was uniform prevalence of the pest across the plot and was building up. At 7 days after first application, the highest number of Spodoptera was recorded in untreated control (4.07/plant). The significant lowest Spodoptera population was observed in T2 (0.30/plant). The treatment T5 was significantly superior over rest of all treatments. The populations of Spodoptera in untreated control increased on 15 days after first application but similar trend was found. At 15 days after first application, there was significantly less number of Spodoptera observed in treatment T2 (0.07/plant). The highest Spodoptera was recorded in control (5.47/plant). The significant lowest number of Spodoptera was recorded in T2, which was superior over rest of all the treatments. Seven days after second application, the Spodoptera population was suppressed in all the treatments except untreated control. The treatment T2 was recorded least number of Spodoptera per plant (0.14), which is significantly lowest among all the treatments and the highest was in untreated control T11 (5.87/plant). Same trend continued at 15 days after second application. At 21 days after second application also similar trend observed. The significant lowest Spodoptera was observed in treatment T2 (0.941/plant), which was superior rest of the treatments. The highest Spodoptera was observed in untreated control (7.20/plant).
TABLE 5
Bio-efficacy of different Insecticides treatments against Spodoptera litura on Okra (Summer-Kharif 2017)
Tr. No Treatment Details Dose
(g a.i./ha) Dose
(ml or g/ha) Pre-count Mean Number of Spodoptera litura /plant
1 DAA 1st spray 7 DAA 1st spray 15 DAA 1st spray 1 DAA 2nd spray 7 DAA 2nd spray 15 DAA 2nd spray 21 DAA 2nd spray
T1 Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG 20+5+375 500 2.54 (1.74) 2.47 (1.72) 0.94 (1.2) 1.27 (1.33) 1.2 (1.31) 1.67 (1.47) 2.07 (1.6) 2.4 (1.71)
T2 Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG 25+6.25+375 500 2.34 (1.66) 1.8 (1.51) 0 (0.71) 0.07 (0.76) 0 (0.71) 0.14 (0.8) 0.4 (0.95) 0.94 (1.2)
T3 Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG 30+7.50+350 500 3.14 (1.9) 1.87 (1.54) 0 (0.71) 0.14 (0.79) 0 (0.71) 0.07 (0.76) 0.54 (1.02) 1.14 (1.27)
T4 Dinotefuran 20% SG + Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 30+8.5+585 150+170+ 780 3.8 (2.05) 2.14 (1.63) 0.14 (0.79) 0.2 (0.84) 0.07 (0.76) 0.34 (0.91) 0.54 (1.02) 1.07 (1.26)
T5 Dinotefuran 20% SG + Emamectin Benzoate 5% SG (Tank-Mix) 30+8.5 150+170 2.7 (1.79) 2.47 (1.72) 0.00 (0.71) 1.74 (1.49) 1.14 (1.26) 1.54 (1.42) 1.8 (1.51) 3.67 (2.01)
T6 Dinotefuran 20% SG + Acephate 75% SP (Tank-Mix) 30+585 150+780 3.94 (2.11) 3.74 (2.06) 2.07 (1.6) 2.87 (1.84) 2.8 (1.82) 3.4 (1.98) 3.8 (2.08) 4.47 (2.23)
T7 Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 8.5+585 170+780 2.27 (1.64) 1.54 (1.4) 0.34 (0.91) 1.07 (1.25) 0.54 (1.02) 1.00 (1.23) 1.27 (1.33) 1.8 (1.52)
T8 Dinotefuran 20% SG 30 150 2.27 (1.62) 2.27 (1.62) 2.6 (1.76) 3.94 (2.11) 3.87 (2.09) 4.2 (2.17) 4.67 (2.27) 5.2 (2.39)
T9 Emamectin Benzoate 5% SG 8.5 170 3.74 (2.06) 2.67 (1.78) 0.34 (0.91) 1.8 (1.51) 0.94 (1.19) 1.34 (1.34) 1.67 (1.47) 2.07 (1.61)
T10 Acephate 75% SP 585 780 3.34 (1.95) 3.07 (1.88) 2.74 (1.8) 3.6 (2.03) 3 (1.87) 3.47 (1.99) 3.94 (2.11) 4.67 (2.28)
T11 Control - - 3.6 (2.02) 3.67 (2.04) 4.07 (2.14) 5.47 (2.45) 5.6 (2.47) 5.87 (2.53) 6.27 (2.61) 7.2 (2.78)
CD (P=0.05) NS 0.4 0.22 0.24 0.22 0.26 0.22 0.29
DAA-Days after Application; NS-Non Significant

3. TO STUDY EFFECT OF TEST INSECTICIDES ON YIELD OF OKRA.
Test Products:
• Dinotefuran 4% + Emamectin benzoate 1% + Acephate 75% SG
• Dinotefuran 5% + Emamectin benzoate 1.25% + Acephate 75% SG
• Dinotefuran 6% + Emamectin benzoate 1.50% + Acephate 70% SG
Test Crop: Abelmoschus esculentus; Variety/Cultivar: Okra, Arka Anamika
Plot Size: 10 m x 5 m (50 sq. m.)
Spacing: 90 cm x 90 cm
Okra fruits from each net plot was recorded at 3 pickings and weighed separately. Three pickings were carried out and at the end of last picking, total yield from each net plot was calculated and computed on hectare basis (tonnes/ha) and statistical analyzed the data.

RESULTS:
As illustrated in Table 6, all the treatments significantly increase the yield than Untreated Control (11.87 ton/ha). The highest yield was observed in treatment T2 (20.43 ton/ha), which was significantly superior over rest of the premix, solo treatment and tank-mix combinations. All test insecticide treatments (T1 to T3) were significantly superior over two way & solo insecticide treatments and tank-mix combination treatment (T4).
TABLE 6
Effect of different Insecticides treatments on Okra Yield (Summer-Kharif 2017)
Tr. No Treatment Details Dose (g a.i./ha) Dose (ml or g/ha) Yield (Tones/ha)

T1 Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG 20+5+375 500 18.53
T2 Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG 25+6.25+375 500 20.43
T3 Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG 30+7.50+350 500 18.93
T4 Dinotefuran 20% SG + Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 30+8.5+585 150+170+780 20.00
T5 Dinotefuran 20% SG + Emamectin Benzoate 5% SG (Tank-Mix) 30+8.5 150+170 17.47
T6 Dinotefuran 20% SG + Acephate 75% SP (Tank-Mix) 30+585 150+780 18.23
T7 Emamectin Benzoate 5% SG + Acephate 75% SP (Tank-Mix) 8.5+585 170+780 17.00
T8 Dinotefuran 20% SG 30 150 15.80
T9 Emamectin Benzoate 5% SG 8.5 170 14.13
T10 Acephate 75% SP 585 780 13.87
T11 Control - - 11.87
CD (P = 0.05) 1.31

4. TO STUDY PHYTOTOXICITY EFFECT OF TEST INSECTICIDES ON OKRA.
Test Products:
• Dinotefuran 4% + Emamectin benzoate 1% + Acephate 75% SG
• Dinotefuran 5% + Emamectin benzoate 1.25% + Acephate 75% SG
• Dinotefuran 6% + Emamectin benzoate 1.50% + Acephate 70% SG
Test Crop: Abelmoschus esculentus; Variety/Cultivar: Okra, Arka Anamika
Number of Treatments: 11
Number of Applications: Two applications at an interval of 15 days
Observations were taken on damage caused to plants, if any, by the application of different treatments by taking into the account phytotoxic symptoms viz. leaf injury on tips and leaf surface, wilting, vein clearing, necrosis, epinasty and hyponasty on ten plants per plot. The observations were recorded before spray and 1, 3, 5, 7 & 10th day after applications. For Phytotoxicity study on leaf injury on tips and leaf surface the Scale (0-10) used is given below.
PHYTOTOXICITY RATING SCALE (PRS)
CROP RESPONSE/ CROP INJURY RATING
0-00 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10
RESULTS:
The test insecticide combination were sprayed to check the phytotoxic effects like leaf injury on tips/surface, vein clearing, necrosis, hyponasty and epinasty on the Okra crop at X and 2X dosages. The observations on these phytotoxicity parameters were observed on before spray and at 3, 5, 7 and 10 days after application. But there was no any phytotoxicity observed on the crop after spraying in any treatment. There was no any adverse effect noticed on the crop in the field applied with theses insecticide combinations (TABLE 7).
TABLE 7
Phytotoxicity effect of different Insecticide treatments on Okra (Summer-Kharif 2017)
Tr. No. Treatment Details
Dose *Phytotoxicity
(Based on 0-10 Phytotoxicity Rating Scale)
g a.i./ha ml or g/ha Before Spray Days after application (DAA)
1 3 5 7 10
T1 Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG 20+5+375 500 0 0 0 0 0 0
T2 Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG 25+6.25+375 500 0 0 0 0 0 0
T3 Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG 30+7.50+350 500 0 0 0 0 0 0
T4 Dinotefuran 4% + Emamectin Benzoate 1.00% + Acephate 75% SG 40+10+750 1000 0 0 0 0 0 0
T5 Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG 50+12.5+750 1000 0 0 0 0 0 0
T6 Dinotefuran 6% + Emamectin Benzoate 1.50% + Acephate 70% SG 60+15.0+700 1000 0 0 0 0 0 0
T7 Control - - 0 0 0 0 0 0

CONCLUSION:

1. The combinations of insecticide, Dinotefuran, Emamectin Benzoate and Acephate give effective control of sucking pests and Lepidopteran pests of Okra. Among the different 3 ratios of this combination, Dinotefuran 5% + Emamectin Benzoate 1.25% + Acephate 75% SG is performing better than rest ratios.
2. Comparatively, efficacy of readymade test insecticide combinations (Dinotefuran + Emamectin Benzoate + Acephate) is far better than market standards- Dinotefuran 20% SG & Emamectin Benzoate 20% SG, Acephate 75% WP, tank-mix combination and their two way combinations for controlling sucking pest complex of Okra.
3. The test insecticide combinations produce higher Okra yield.
4. There is no phytotoxicity effect of test insecticide combinations on Okra.
5. Overall, the test insecticides show synergistic effect for controlling the sucking and Lepidopteran pests of Okra. The test insecticides can be used effectively and safely for the management of sucking and lepidopteran pests as compared to two way or individual Dinotefuran, Emamectin benzoate and Acephate and tank-mix combinations of all the three.

TECHNICAL ADVANCEMENTS

The present disclosure described herein above has several technical advantages including, but not limited to, the realization of a synergistic insecticidal composition which is stable and is in a ready-to use form. The present disclosure further provides an easy and simple process for the preparation of the synergistic insecticidal composition.

Throughout this specification the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

The use of the expression “at least” or “at least one” suggests the use of one or more elements or ingredients or quantities, as the use may be in the embodiment of the invention to achieve one or more of the desired objects or results. While certain embodiments of the inventions have been described, these embodiments have been presented by way of example only, and are not intended to limit the scope of the inventions. Variations or modifications to the formulation of this invention, within the scope of the invention, may occur to those skilled in the art upon reviewing the disclosure herein. Such variations or modifications are well within the spirit of this invention.

The numerical values given for various physical parameters, dimensions and quantities are only approximate values and it is envisaged that the values higher than the numerical value assigned to the physical parameters, dimensions and quantities fall within the scope of the invention unless there is a statement in the specification to the contrary.

While considerable emphasis has been placed herein on the specific features of the preferred embodiment, it will be appreciated that many additional features can be added and that many changes can be made in the preferred embodiment without departing from the principles of the disclosure. These and other changes in the preferred embodiment of the disclosure will be apparent to those skilled in the art from the disclosure herein, whereby it is to be distinctly understood that the foregoing descriptive matter is to be interpreted merely as illustrative of the disclosure and not as a limitation. ,CLAIMS:WE CLAIM:

1. A synergistic insecticidal composition comprising:
a. a neonicotinoid compound in an amount in the range of 1% to 80% of the total mass of the composition;
b. an organophosphate compound in an amount in the range of 1% to 90% of the total mass of the composition;
c. an avermectin compound in an amount in the range of 1% to 10% of the total mass of the composition; and
d. at least two agrochemically acceptable excipients in an amount in the range of 0.5% to 75% of the total mass of the composition.

2. The synergistic insecticidal composition as claimed in claim 1, wherein the neonicotinoid compound is selected from the group consisting of acetamiprid, clothianidin, imidacloprid, nitenpyram, dinotefuran, thiacloprid and thiamethoxam.

3. The synergistic insecticidal composition as claimed in claim 1, wherein the organophosphate compound is selected from the group, consisting of, acephate, demeton-S-methyl, dimethoate, parathion-methyl, phenoate, phorate, profenofos, quinalphos, triazophos, fenitrothion, fenthion, monocrotophos and oxydemeton-methyl.

4. The synergistic insecticidal composition as claimed in claim 1, wherein the avermectin compound is selected from the group consisting of, ivermectin, selamectin, doramectin, emamectin, emamectin benzoate and abamectin.

5. The synergistic insecticidal composition as claimed in claim 1, wherein the agrochemically acceptable excipients are selected from the group consisting of dispersing agent, wetting agent, binder, stabilizing agent, defoamer, polymeric film forming agent and filler.

6. The synergistic insecticidal composition as claimed in claim 1, wherein the agrochemically acceptable excipients comprise:
a. a first excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent; and
b. a second excipient comprising at least one stabilizing agent and a filler.

7. The synergistic insecticidal composition as claimed in claim 6, wherein the second excipient comprises at least two stabilizing agents and a filler.

8. The synergistic insecticidal composition as claimed in claim 1, 6 and 7, wherein the stabilizing agent is selected from the group consisting of phosphoric acid monoesters, phosphoric acid diesters, phosphoric acid mono- and di- ester mixture, sodium phosphate, butylated hydroxytoluene, castor oil ethoxylate, ethoxylated hydrogenated castor oil, vegetable oil and epoxidized soyabean oil.

9. The synergistic insecticidal composition as claimed in claimed in claim 1, 6 and 7, wherein the stabilizing agent is a combination of phosphoric acid (mono and di ester mixture) and sodium phosphate in a pre-determined ratio.

10. The synergistic insecticidal composition as claimed in claim 1, wherein said composition comprises:
a. a neonicotinoid compound in an amount in the range of 1% to 80% of the total mass of the composition;
b. an organophosphate compound in an amount in the range of 1% to 90% of the total mass of the composition;
c. an avermectin compound in an amount in the range of 0.1% to 10% of the total mass of the composition;
d. a first excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent; and
e. a second excipient comprising
i) at least one stabilizing agent selected from phosphoric acid mono and di ester mixture and sodium phosphates in an amount in the range of 0.2% to 5% of the total mass of the composition; and
ii) a filler in an amount in the range of 1% to 30% of the total mass of the composition.

11. The synergistic insecticidal composition as claimed in claim 1, wherein said composition comprises:
a. Dinotefuran, in an amount in the range of 1% to 50 % of the total mass of the composition;
b. Acephate, in an amount in the range of 30% to 75 % of the total mass of the composition;
c. Emamectin Benzoate, in an amount in the range of 0.5% to 7% of the total mass of the composition; and
d. at least two agrochemically acceptable excipients in an amount in the range of 0.5% to 50% of the total mass of the composition.

12. The synergistic insecticidal composition as claimed in claim 1, wherein said composition comprises:
a. Dinotefuran, in an amount in the range of 1% to 50 % of the total mass of the composition;
b. Acephate, in an amount in the range of 30% to 75 % of the total mass of the composition;
c. Emamectin Benzoate, in an amount in the range of 0.5% to 7% of the total mass of the composition;
d. agrochemically acceptable first excipient comprising polymeric film-forming agent in an amount in the range of 0.1% to 10% of the total mass of the composition; and
e. agrochemically acceptable second excipient comprising at least one stabilizing agent selected from phosphoric acid mono- and di- ester mixture and sodium phosphates in an amount in the range of 0.2% to 5% of the total mass of the composition and filler in an amount in the range of 1% to 30% of the total mass of the composition.

13. The synergistic insecticidal composition as claimed in claim 1, wherein said composition is in at least one dosage form selected from the group consisting of soluble granules, encapsulated granules, water dispersible granules, suspension concentrate, emulsifiable concentrate and soluble powder.

14. The synergistic insecticidal composition a claimed in claim 1, wherein said composition is a tank-mix.

15. A process for preparation of a synergistic insecticidal composition, said process comprising:
f. blending pre-determined quantities of an organophosphate compound, a neonicotinoid compound and an agrochemically acceptable first excipient, selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent, together to obtain a first substantially homogenized mixture;
g. blending pre-determined quantities of an avermectin compound, at least one stabilizing agent and a filler to obtain a second substantially homogenized mixture;
h. mixing the first substantially homogenized mixture with the second substantially homogenized mixture to obtain a third homogenized mixture;
i. pounding the third homogenized mixture to a fine particle size in the range of 1 µm to 10 µm to obtain a substantially homogenized powder; and
j. converting the substantially homogenized powder into at least one dosage form selected from the group consisting of soluble granules, encapsulated granules, water dispersible granules, suspension concentrate, emulsifiable concentrate, soluble powder and a tank-mix.

16. A method of controlling and eliminating insects from a pre-determined area, said method comprising applying to a pest, to a locus of a pest or to a plant susceptible to attack by a pest, an effective amount of the synergistic insecticidal composition as claimed in claims 1 to 14.

17. An insecticidal kit, said insecticidal kit comprising,
d. a first sachet containing a first substantially homogenized mixture comprising predetermined quantities of an organophosphate compound, a neonicotinoid compound and an agrochemically acceptable first excipient selected from the group consisting of dispersing agent, wetting agent, binder, defoamer and polymeric film-forming agent; and
e. a second sachet containing a second substantially homogenized mixture comprising pre-determined quantities of an avermectin compound, at least one stabilizing agent and a filler; and
f. an instruction pamphlet;
wherein the contents of the first sachet and the second sachet are to be mixed in accordance with the instructions provided in the instruction pamphlet.