DESC:Technical field of the Invention:
The present invention relates to an antimicrobial wipe comprising an antimicrobial lysis solution that can lyse bacterial as well as fungal dwellers on gadgets. More particularly, the invention relates to an antimicrobial wipe that can lyse bacterial as well as fungal dwellers on gadgets that serve as a source for transmitting microbes from one human being to other by exchange of the same.

Background and Prior art of the Invention:
Gadgets have become an indispensable part of human beings for instant communication and social access. Day by day its users and applications are growing high. Along with the health hazard of emitted radiation from the device, it has another health risk of transmitting microbes from hand to hand. As these devices have constant contact with skin, face, mouth, ears and hands, an optimum moisture content and temperature of human body is maintained on it, which make it an open breeding space for transmission of microorganisms. Children use the gadget of adults and these organisms are transmitted to them also. Hence germ infected gadgets pose health issues to human kind. Health workers contribute more to the transmission of microbes if they use gadgets at their work places.

In order to reduce the risk or stop the transmission, a periodic cleaning is required for our gadgets. Thus, there is a requirement of a solution that wipe out all bacteria and fungus present on the surface of these gadgets so that the transmission of many infectious diseases can prevent.

The significance of bacterial contamination of mobile phones from health care units and impact of transmission among people has been discussed in publications such as Heyba et al., BMC infectious Diseases (2015), Selim et al., GMS Hygiene and infection Control (2015), Vol 10, S. Zakai et al. Journal of Microscopy and Ultrastructure 4 (2016), Chang et al, PLoS ONE 12(5).

The patented literature on bacterial contamination of devices include US7550430 which describes an antimicrobial oligopeptide that is useful in pharmaceutical, health care, medical device, industrial, food, agricultural and personal care applications.
US20130209718 relates to a touch screen film to protect the touch screen surface of hand held electronic devices that can easily been removed from touch screen surface without leaving an adhesive residue on electronic device and prevents the bacteria from growing on surface of electronic device.

US2016/0191095 describes a cell phone case including antimicrobial and/or fragrance properties.

US2002106399 relates to wipes impregnated with an antimicrobial composition having alcohol and a surfactant exhibiting antibacterial and antiviral benefits, as well as kills germs and cleans skin, as well as surfaces.

US9096821 discloses preloaded cleaning and sanitizing wipes comprising a nonwoven substrate, and a cleaning formulation loaded onto or within the nonwoven substrate. The cleaning composition may include an antimicrobial compound comprising quaternary ammonium compound, an alcohol solvent, preservative, glycol solvent surfactants and water.

US7090882 discloses antimicrobial salt solutions for food safety and quality applications comprising surfactant, acid and an inorganic salt.

Research on same problem has already been discussed and initiated by different countries and to an extent certain solutions has come into market so far however, has not been popularized yet. Major studies are done on public hospitals and health care institutes but not in common population.

As of now the available solutions for the challenge are PhoneMop (a 99.99% bacterial killer) and other antibacterial wipes like wireless wipe, and devices like UV sanitizer - Cell Blasters universal UV cell phone sanitizer or Easy care portable multiuser UV sterilizer. The use of PhoneMop and other wipes are for bactericidal only and no fungal clean-up is possible with them. Even though they are in market, a public awareness on this regard has not been done and hence only a very few people are utilizing it. Use of devices for cleaning microbes on phones is costly, difficult to carry everywhere and not popular among the people.

There are about 650 million mobile phone users in India apart from many more gadget users. Health care associated infections are a major challenge to health care system and have reported significant mortality, morbidity and economic loss. A large number of people are working in health care system. The transmission of associated infections from hospitals to common population is a threat and these mobile phones and gadgets are major source for it. The lack of awareness on impact of unhygienic handling of gadgets including mobile phones is also a major issue.

Till this day, research studies have only focused on bacteria that are transmitted through mobile phones and solutions like PhoneMop has come up that can kill bacteria.

Therefore, there remains a need in the art to provide a solution to the problems associated with fungal diversity on the gadgets.

The inventor of the present invention have come up with a lysis solution which is not only focused on bacterial diversity that survives on gadgets, but also equally and more importantly focused on fungal biodiversity, which becomes the object of present invention.

Summary of the Invention:
To meet the above objective, the present invention provides antimicrobial wipe comprising an antimicrobial lysis solution consist of salts that establish ionic strength in buffer solution; salts that regulate acidity and osmolarity of lysate; compounds that destabilizes the integrity of cell wall of microbes; ionic detergents that solubilize cell membranes of microbes and alcohol for disinfecting the surface. The present microbial lysis solution can lyse all cells irrespective of bacteria and fungi and can further inhibit the colonization of these microbes. The present antimicrobial wipe is eco-friendly, made of premium tissue paper and are biodegradable.

Accordingly, in an aspect, the present invention provides antimicrobial wipe comprising antimicrobial lysis solution consist of;
a) Salt selected from Sodium chloride/Potassium chloride in an amount of 25 to 100 mM;
b) salt selected from Tris chloride/Ammonium chloride in an amount of 25 to 100 mM;
c) chelating agent selected from ethylene-diamine-tetra-acetic acid (EDTA) or ethylene glycol tetra-acetic acid (EGTA) in an amount of 10mM to 15mM;
a) detergents such as Sodium dodecyl sulphate and Ethyl trimethyl ammonium bromide in an amount of 0.1 to 0.3% and
b) alcohol selected from isopropanol or ethanol in an amount of 30-50%.

The wipe material made of tissue paper or non-woven fabric is impregnated with present antimicrobial lysis solution.

Brief description of Figures:
Figure 1: Flow diagram showing working of present antimicrobial wipe comprising lysis solution
Figure 2: Amplification of ‘Before’ and ‘After’ samples with Bacterial specific primers
Figure 3: Amplification of ‘Before’ and ‘After’ samples with Fungal specific primers
Figure 4: Bacterial and Fungal ‘Species’ abundance found in ‘Before’ samples
Figure 5: Bacterial and Fungal ‘Genus’ abundance found in ‘Before’ samples
Figure 6: Bacterial and Fungal ‘Family’ abundance found in ‘Before’ samples
Figure 7: Bacterial and Fungal ‘Order’ abundance found in ‘Before’ samples
Figure 8: Bacterial and Fungal ‘Phylum’ abundance found in ‘Before’ samples
Figure 9: Bacterial and Fungal ‘Class’ abundance found in ‘Before’ samples
Figures 10(a) to 10(d): Swabs from gadgets shows inhibition towards adherence of microbes

Detailed description of the Invention:
The invention will now be described in detail in connection with certain preferred and optional embodiments, so that various aspects thereof may be more fully understood and appreciated. However, any skilled person will appreciate the extent to which such embodiments could be extrapolated in practice.

The present invention discloses an antimicrobial wipe comprising an antimicrobial lysis solution that lyse all the bacterial as well fungal dwellers on gadgets, since the gadgets serve as a source for transmitting microbes from one human being to other by exchange of the same.

The present antimicrobial wipe comprising an antimicrobial lysis solution at the same time also inhibits the colonization of bacterial and fungal diversity for a time period of 4-6 days, preferably 4 days so that transmission of many infectious diseases can prevent.

The present invention discloses an antimicrobial wipe comprising antimicrobial lysis solution consist of salts that establish ionic strength in buffer solution; salts that regulate acidity and osmolarity of lysate; compounds that destabilizes the integrity of cell wall of microbes; ionic detergents that solubilize cell membranes of microbes and alcohol for disinfecting the surface.

Accordingly, in a preferred embodiment, the present invention discloses an antimicrobial lysis solution which comprises;
a) Salt selected from Sodium chloride or Potassium chloride in an amount of 25 to 100 mM;
b) salt selected from Tris chloride or Ammonium chloride in an amount of 25 to 100 mM;
c) chelating agent selected from ethylene-diamine-tetra-acetic acid (EDTA) or ethylene glycol tetra-acetic acid (EGTA) in an amount of 10mM to 15mM;
d) detergents such as Sodium dodecyl sulphate (SDS) and Ethyl trimethyl ammonium bromide in an amount of 0.1 to 0.3% and
e) alcohol selected from isopropanol or ethanol in an amount of 30-50%;
wherein said antimicrobial lysis solution wipes out bacterial diversity, eliminates fungal biodiversity and inhibit the colonization of these microbes on gadgets such for a time period, thereby preventing the transmission of many infectious diseases caused by these microbes from one human being to other by exchange of the gadget.
To prepare the antimicrobial lysis solution, the stock solutions of Sodium chloride/Potassium chloride, Tris chloride/Ammonium chloride are made to 1M. The stock solution of Sodium chloride/Potassium chloride is diluted to obtain the concentration ranging from 25 to 100mM. Similarly, the stock solution of Tris chloride/Ammonium chloride is further diluted to obtain the concentration ranging from 25 to 100 mM.

The stock solution of chelating agent is made to 150mM and is further 10 times diluted to obtain the concentration of the chelating agent ranging from 10mM to 15mM.

In another embodiment, the present invention discloses an antimicrobial lysis solution comprises (a) Sodium chloride/Potassium chloride in an amount of 25mM to 100 mM (0.05% to 0.5% or 25ml to 100ml); (b) Tris chloride/Ammonium chloride in an amount of 25mM to 100 mM (0.4% to 1.6% or 25ml to 100ml); (c) chelating agents in an amount of 10mM to 15mM (0.4% to 0.8% or 50ml to 100ml); (d) detergents in an amount of 0.1% to 0.3% (1gm to 3gm); and (e) alcohol in an amount of 30% to 50% (300ml to 500ml).

Accordingly, in another embodiment, the present invention discloses a process for preparation of an antimicrobial lysis solution which comprises;
i. Mixing 25-100 ml of Sodium chloride/Potassium chloride (1M), 25-100 ml of Tris chloride/Ammonium chloride (1M) and 50-100 ml of chelating agents (150mM) and making the volume up to 400 ml with sterile water;
ii. adding 1gm - 3gm of detergents followed by mixing and making the volume up to 500 ml using sterile water; and
iii. adding 300-500ml alcohol to step (ii) and making the final volume up to 1 liter with sterile water.

In another embodiment, the present invention discloses antimicrobial lysis solution comprises sodium chloride or potassium chloride that establishes the ionic strength in buffer solution. The tris chloride or ammonium chloride regulates the acidity and osmolarity of the lysate. Chelating agents like ethylene diamine tetra acetic acid (EDTA) or ethylene glycol tetra acetic acid (EGTA) bind to metal ions with two positive charges (e.g. magnesium and calcium), thereby making them unavailable for other reactions. Disodium EDTA or Calcium Disodium EDTA help to maintain the integrity of cell membrane, eliminating them with EDTA/EGTA destabilises the membrane. Along with Tris Chloride/Ammonium Chloride, EDTA/EGTA regulates acidity and osmolarity of the lysate. Ionic detergents like Sodium dodecyl sulphate (SDS) and cationic detergents like Ethyl trimethyl ammonium bromide can solubilise cell membrane and thereby denatures most of the protein in cells in turn disrupt protein function of cells. Isopropanol/ethanol is added for disinfecting the surface in addition to all other cell lysing agents.

In another embodiment, the present antimicrobial lysis solution is impregnated on wipe material and these wipes are folded and tightly packed individually for easy use. The present antimicrobial wipes are eco-friendly, made of premium tissue paper and are biodegradable.

The wipe used according to present invention is made of synthetic analogues of any suitable tissue paper or combination of materials, such as, non-woven fabric similar to the type used in diapers and dryer sheets, made by weaving together fibers of silk, cotton, polyester, wool, and similar materials to form an interlocking matrix of loops or any combination thereof. It can also be made up of hydrophilic Spunlace Nonwoven Fabric, PVC synthetic leather non-woven fabric, or Spunlace dependent on any other parameters.

In another embodiment, the present invention discloses an antimicrobial wipe comprising an antimicrobial lysis solution that lyse all the bacterial as well fungal diversity on gadgets; wherein said gadgets are selected form smartphone/mobile, tablet, smart watch, laptop, or screen of calculator; preferably, a mobile or a tablet.

The following Table 1 and Table 2 describe the bacterial and fungal diversity and its impact of ‘Before’ samples on human health.

Table 1: Bacterial diversity and its impact of “Before” samples

Bacteria Impact
Bacillus The different species of Bacillus produce a variety of extracellular products including antimicrobial substances, enzymes, pigments, and toxins in few species. Two different types of enterotoxins are produced by B. cereus during exponential growth: the enterotoxin causing diarrhea and the emetic toxin. Phospholipase C produced by Bacillus cereus may have a secondary role in ocular infections by disrupting host cell membrane phospholipids exposed by the action of other toxins. Endocarditis caused by Bacillus organisms is a well-recognized complication of intravenous drug abuse. Musculoskeletal infections caused by Bacillus species have been reported. B. cereus is the major isolate from cultures of wound and bone biopsies from infected sites. A wide variety of Bacillus species have been isolated from cerebrospinal fluid of patients who had spinal anesthesia, subdural hematoma, ventricular shunts and parameningeal foci of infections e.g. otitis and mastoiditis.
Lactobacillus Friendly bacteria that normally live in our digestive, urinary, and genital systems without causing disease.
Streptococcus In addition to streptococcal pharyngitis (strep throat), certain Streptococcus species are responsible for many cases of pink eye, meningitis, bacterial pneumonia, endocarditis, erysipelas, and necrotizing fasciitis (the 'flesh-eating' bacterial infections). They are commonly found in nose, skin, and genital areas. These bacteria can destroy red blood cells or damage them. Group A and B are associated with disease. Group A causes strep throat, skin infections and toxic shock syndrome and necrotizing fasciitis. Group B causes life-threatening diseases in new borns and pregnant women.
Weissella Weissella strains have been isolated from clinical specimens such as blood, skin, infected wounds and feces of both humans and animals, described as opportunistic pathogens of humans or as emerging pathogen for farmed rainbow trout.
Micrococcus Skin infections are caused by Micrococcus luteus. The skin infections, or chronic cutaneous infections, result in pruritic eruptions of the skin in some areas as well as scattered papule lesions with or without central ulcerations.
Acinetobacter There are many different species of Acinetobacter that can cause disease, but A. baumannii accounts for about 80 percent of reported Acinetobacter infections. Healthy people have a very low risk of getting an A. baumannii infection.
Enterococcus Enterococci have emerged as important healthcare-associated pathogen. Enterococci can cause a variety of infections. However, for other types of infections, most notably endocarditis and bacteremia, enterococci can clearly cause serious and often life-threatening disease (Urinary Tract Infections, Intra-Abdominal, Pelvic, and Soft Tissue Infections, Bacteremia, Endocarditis etc.).
Paracoccus The genus Paracoccus is one of the most distantly related of the Proteobacteria to Escherichia coli. Paracoccus yeei, a gram-negative bacterium, has been identified as an unusual etiologic opportunistic agent in patients with cardiac transplant, automated peritoneal dialysis, or corneal graft. Case of Paracoccus yeei infection documented in a transplanted heart.
Haemophilus There are several types of Haemophilus. They can cause different types of illnesses involving breathing, bones and joints, and the nervous system. One common type, HIB (Haemophilus influenzae type b), causes serious disease. It usually strikes children under 5 years old.
Pseudomonas Psuedmonas are fairly common pathogens involved in infections acquired in a hospital setting. They usually do not cause any infections in healthy people.
Corynebacterium The genus contains the species Corynebacterium diphtheriae and the Nondiphtherial corynebacteria, collectively referred to as diphtheroids. Nondiphtherial corynebacteria, originally thought to be mainly contaminants, have increasingly over the past 2 decades been recognized as pathogenic, especially in immunocompromised hosts. They usually are not pathogenic but can occasionally opportunistically capitalize on atypical access to tissues (via wounds) or weakened host defenses.
Roseomonas Roseomonas are pathogenic to humans, causing bacteremia, and wound urinary tract and other infections. These are set of organisms found in blood with about 20% from wounds, exudates, and abscesses and about 10% from genitourinary sites. They normally affect immunosuppressed patients or children below 10yrs.
Gemella Gemella species are part of normal human flora. As opportunistic pathogens, they can cause life-threatening infection in individuals with risk factors.
Klebsiella Klebsiella infections refer to several different types of healthcare-associated infections that are all caused by the Klebsiella bacteria, including pneumonia; bloodstream infections; wound or surgical site infections; and meningitis. Healthy people usually do not get Klebsiella infections.
Gordonia Rarely cause infection in humans. Line-related bacteremia’s that occur in patients with acute myelogenous or acute lymphocytic leukemia.
Kocuria Kocuria species have now emerged as human pathogens, mostly in compromised hosts with severe underlying disease.
Porphyromonas Most Porphyromonas spp. was isolated from abscesses, pulmonary infections, ear infections, wound infections, peritonitis, paronychia and chronic sinusitis.

Table 2: Fungal diversity and its impact of “Before” samples

Fungus Impact
Aspergillus penicillioides Aspergillus penicillioidesis a common indoor fungus in damp buildings where it has been associated with allergic rhinitis, allergic reactions, asthma and skin allergies.
Candida parapsilosis Candida species are the fourth leading cause of nosocomial bloodstream infection. C. parapsilosis is often the second most commonly isolated Candida species from blood cultures. Immunocompromised individuals such as AIDS patients and surgical patients, particularly those having surgery of the gastrointestinal tract, are at high risk for infection with C. parapsilosis.
Alternaria brassicae They are also common allergens in humans, growing indoors and causing hay fever or hypersensitivity reactions that sometimes lead to asthma. They readily cause opportunistic infections in immunocompromised people such as AIDS patients.
Hortaea werneckii Hortaea werneckii is the causative agent of Tinea nigra. Tinea nigra is a superficial infection of stratum corneum. The infection is mostly acquired via direct inoculation of the fungus onto the skin due to contact with soil, wood, and decaying vegetation. The lesions of tinea nigra are usually located on palms but may occasionally involve other parts of the body, such as soles of the feet. Tinea nigra is a superficial mycosis of the palms that is most often caused by Hortaea werneckii.
Sagenomella sp
HMH 2007a Sagenomella species normally cause unspecific symptoms and infections of Sagenomella are difficult to diagnose because this is an uncommon genus of fungus. S. keratitidis was found to cause inflammation of the cornea, or keratitis, in an individual who wore contact lenses.
Malassezia sp
M9959 The genus Malassezia contains three member species: Malassezia furfur and Malassezia sympodialis, both obligatory lipophilic, skin flora yeasts of humans, and Malassezia pachydermatis, non-obligatory lipophilic, skin flora yeast of other warm-blooded animals. They have been reported as agents of more invasive human diseases including deep-line catheter-associated sepsis.
Lasiodiplodia theobromae Lasiodiplodia theobromae, a common tropical phytopathogen mainly known previously as a rare agent of keratitis and onychomycosis in humans. Phaeohyphomycosis is a well-recognized category of fungal diseases caused by species producing dark walled, melanized filaments in human tissue.
Kodamaea
ohmeri Kodamaea (Pichia) ohmeri was formerly considered a contaminant, but is now known to be a significant human pathogen that has been shown to cause fungemia, endocarditis, funguria, and peritonitis in immunocompromised patients.
Capnodiales sp TR006 Capnodiales is a diverse order of Dothideomycetes, A small number of these fungi are also able to parasitise humans and animals, including species able to colonise human hair shafts.
Rhodotorula minuta R. minuta are less frequently isolated from natural environments. R. minuta are known to cause disease in humans.
Trichosporon asahii Trichosporon asahii (Trichosporon beigelii) infections are rare but have been associated with a wide spectrum of clinical manifestations, ranging from superficial involvement in immunocompetent individuals to severe systemic disease in immunocompromised patients.
Rhodotorula mucilaginosa Rhodotorula mucilaginosa was the most common species of fungemia. Rhodotorula mucilaginosa causes Aortic homograft endocarditis. They affect mostly immunocompromised patients.
Setosphaeria rostrata Setosphaeria rostrata is a thermophilic fungus with an asexual reproductive form treated in the genus Exserohilum etosphaeria. It is one of the 35 Exserohilum species implicated uncommonly as opportunistic pathogens of humans where it is an etiologic agent of sinusitis, keratitis and CNS vasculitis as well as cutaneous and subcutaneous mycoses. Exserohilum are most often seen in regions with hot climates.
Paecilomyces sp HF12071 Infections in apparently immunocompetent patients have also been reported.
Clavispora lusitaniae Clavispora lusitaniae [named until 1996 Candida (C.) lusitaniae] were emerging fungal organisms. Fungaemias are caused due to Clavispora lusitaniae. This yeast can cause systemic infection and even death.
Sordariomycetes sp SAB 2009a Sordariomycetes is from the Latin sordes (filth) because some species grow in animal feces, though growth habits vary widely across the class. Members of this group can grow in soil, dung, leaf litter, and decaying wood as decomposers, as well as being fungal parasites, and insect, human, and plant pathogens.
Penicillium citrinum Although Penicillium citrinum is a fungus recognized to be ubiquitous in the environment, it has only rarely been reported as a cause of human infection. Only affects immune compromised patients.

From the above Table 1 and Table 2 it is observed that there is presence of thousands of bacteria and fungus on the gadgets which are harmful for human health, hence, it is necessity to get rid of these microbes. The sequence of the microbial diversity of samples pooled, deposited in NCBI with SRA submission: SUB5568208, under Bio-project: PRJNA541262, Bio-sample SAMN11581036; SRA Accessions: Bacteria (SRR9017026) and Fungi (SRR9017027). Therefore the present invention provides an antimicrobial wipe comprising an antimicrobial lysis solution that can wipes out bacterial diversity, eliminates fungal biodiversity and inhibit the colonization of these microbes on gadgets for a time period, thereby preventing the transmission of many infectious diseases caused by these microbes from one human being to other by exchange of the gadget.

Accordingly, in yet another embodiment, the present invention provides a study in a population of about 200 individuals (Figure 1). The sterile swabs were used to collect the microbes from gadgets and then wiped with the antimicrobial wipe comprising antimicrobial lysis solution of present invention. After 5 minutes again the remaining microbes were collected with new sterile swab.

‘Before wipe’ and ‘after wipe’ samples were subjected to metagenome analysis utilizing V3-V4 hypervariable region for bacterial diversity (Figure 2) and ITS (Internal Transcribed Spacer) region for fungal diversity (Figure 3).

The V3-V4 region of the 16S rRNA is known to indicate a diversity of bacterial taxa present in a biological sample. It is observed from Figure 2 that the ‘before wipe’ sample showed amplification as ‘bands at 500 bp’, whereas, the ‘after wipe’ samples showed ‘no bands’, therefore, indicating excellent anti-bacterial activity of the present anti-microbial lysis solution.
Similarly in Figure 3, ITS region markers were used to determine the fungal diversity in ‘before wipe’ and ‘after-wipe’ samples. Accordingly, the ‘before wipe’ samples showed amplification as band, whereas the ‘after wipe’ sample showed no band, therefore, indicating excellent anti-fungal activity of the present anti-microbial lysis solution.

‘Before’ samples amplified well confirming the bacterial and fungal diversity and respective libraries were prepared and subjected to sequencing in Illumina chemistry. The sequence result confirmed the presence of thousands of bacteria and fungus (Figures 4, 5, 6, 7, 8 and 9) that had a proportion of harmful ones. ‘After’ samples does not amplify at all confirming the absence of microbes.

In yet another embodiment, the present invention discloses that the swabs from gadgets which are already treated with present antimicrobial lysis solution were taken after a time period of 4 days and then plated on LB (Luria broth) agar and potato dextrose agar. There is no growth of any microbes observe even after 24 hours of incubation. This shows the inhibition towards the adherence of microbes for a time period of 4 days [Figures 10(a) to 10(d)].

The results shown in Figure 10(a) to Figure 10(d) are obtained after treating the swabs from gadgets with antimicrobial lysis solution of Example 8.

In yet another embodiment, the present invention discloses a method of lyse all bacterial as well as fungal biodiversity and inhibition of colonization of these microbes on gadget by applying antimicrobial lysis solution comprising salt selected from Sodium chloride or Potassium chloride; salt selected from Tris chloride or Ammonium chloride; chelating agent selected from ethylene-diamine-tetra-acetic acid (EDTA) or ethylene glycol tetra-acetic acid (EGTA); detergents such as Sodium dodecyl sulphate and Ethyl trimethyl ammonium bromide and alcohol selected from isopropanol or ethanol, on the surface of the gadget.

In yet another embodiment, the present invention discloses use of antimicrobial lysis solution comprising salt selected from Sodium chloride or Potassium chloride; salt selected from Tris chloride or Ammonium chloride; chelating agent selected from ethylene-diamine-tetra-acetic acid (EDTA) or ethylene glycol tetra-acetic acid (EGTA); detergents such as Sodium dodecyl sulphate and Ethyl trimethyl ammonium bromide and alcohol selected from isopropanol or ethanol, for lyse all the bacterial as well fungal diversity on gadgets and also inhibits the colonization of these microbes on gadgets, thereby preventing the transmission of many infectious diseases caused by these microbes from one human being to other by exchange of the gadget.

In yet another embodiment, the present invention discloses a kit comprises wipe material impregnated with antimicrobial lysis solution comprising salt selected from Sodium chloride or Potassium chloride; salt selected from Tris chloride or Ammonium chloride; chelating agent selected from ethylene-diamine-tetra-acetic acid (EDTA) or ethylene glycol tetra-acetic acid (EGTA); detergents such as Sodium dodecyl sulphate and Ethyl trimethyl ammonium bromide and alcohol selected from isopropanol or ethanol; wherein said wipes are folded and tightly packed individually for easy use.

Examples:
Some typical examples illustrating the embodiments of the present invention are provided; however, these are exemplary only and should not be regarded as limiting the elements of the present invention.

Example 1: Antimicrobial lysis Solution 1
Sr. No. Ingredients Quantity
1. Sodium chloride/Potassium chloride 25 to 100 mM
2. Tris chloride/Ammonium chloride 25 to 100 mM
3. Ethylene-diamine-tetra-acetic acid (EDTA) or
Ethylene glycol tetra-acetic acid (EGTA) 10mM to 15mM
4. Sodium dodecyl sulphate and
Ethyl trimethyl ammonium bromide 0.1 to 0.3%
5. Isopropanol or ethanol 30-50%
6. Sterile water Q.S.

Example 2: Antimicrobial lysis Solution 2
Sr. No. Ingredients Quantity
1. Sodium chloride 25 to 100 mM
2. Tris chloride 25 to 100 mM
3. Ethylene-diamine-tetra-acetic acid (EDTA) or
Ethylene glycol tetra-acetic acid (EGTA) 10mM to 15mM
4. Sodium dodecyl sulphate and
Ethyl trimethyl ammonium bromide 0.1 to 0.3%
5. Isopropanol or ethanol 30-50%
6. Sterile water Q.S.

Example 3: Antimicrobial lysis Solution 3
Sr. No. Ingredients Quantity
1. Sodium chloride 25 to 100 mM
2. Ammonium chloride 25 to 100 mM
3. Ethylene-diamine-tetra-acetic acid (EDTA) or
Ethylene glycol tetra-acetic acid (EGTA) 10mM to 15mM
4. Sodium dodecyl sulphate and
Ethyl trimethyl ammonium bromide 0.1 to 0.3%
5. Isopropanol or ethanol 30-50%
6. Sterile water Q.S.

Example 4: Antimicrobial lysis Solution 4
Sr. No. Ingredients Quantity
1. Potassium chloride 25 to 100 mM
2. Tris chloride 25 to 100 mM
3. Ethylene-diamine-tetra-acetic acid (EDTA) or
Ethylene glycol tetra-acetic acid (EGTA) 10mM to 15mM
4. Sodium dodecyl sulphate and
Ethyl trimethyl ammonium bromide 0.1 to 0.3%
5. Isopropanol or ethanol 30-50%
6. Sterile water Q.S.
Example 5: Antimicrobial lysis Solution 5
Sr. No. Ingredients Quantity
1. Potassium chloride 25 to 100 mM
2. Ammonium chloride 25 to 100 mM
3. Ethylene-diamine-tetra-acetic acid (EDTA) or
Ethylene glycol tetra-acetic acid (EGTA) 10mM to 15mM
4. Sodium dodecyl sulphate and
Ethyl trimethyl ammonium bromide 0.1 to 0.3%
5. Isopropanol or ethanol 30-50%
6. Sterile water Q.S.

Example 6: Antimicrobial lysis Solution 6
Sr. No. Ingredients Quantity
1. Sodium chloride/Potassium chloride 0.05 to 0.5%
2. Tris chloride/Ammonium chloride 0.4 to 1.6%
3. Ethylene-diamine-tetra-acetic acid (EDTA) or
Ethylene glycol tetra-acetic acid (EGTA) 0.4 to 0.8%
4. Sodium dodecyl sulphate and
Ethyl trimethyl ammonium bromide 0.1 to 0.3%
5. Isopropanol or ethanol 30-50 %
6. Sterile Water Q.S.
Total 100%

Example 7: Antimicrobial lysis Solution 7
Sr. No. Ingredients Quantity
1. Sodium chloride/Potassium chloride 25 to 100 ml
2. Tris chloride/Ammonium chloride 25 to 100 ml
3. Ethylene-diamine-tetra-acetic acid (EDTA) or
Ethylene glycol tetra-acetic acid (EGTA) 50 to 100 ml
4. Sodium dodecyl sulphate and
Ethyl trimethyl ammonium bromide 1gm to 3gm
5. Isopropanol or ethanol 300 to 500 ml
6. Sterile water Q.S.
Total 1000ml

Example 8: Antimicrobial lysis Solution 8
Sr. No. Ingredients Quantity
1. Sodium chloride 50mM
2. Tris chloride 50mM
3. Ethylene-diamine-tetra-acetic acid (EDTA) 10mM
4. Sodium dodecyl sulphate 0.1%
5. Isopropanol or ethanol 30%
6. Sterile water Q.S.

Example 9: Process for preparation of antimicrobial lysis solution
i. Mixing 25-100ml of Sodium chloride/Potassium chloride (1M), 25-100ml of Tris chloride/Ammonium chloride (1M) and 50-100ml of chelating agents (150mM) and making the volume up to 400ml with sterile water;
ii. adding 1gm-3gm of detergents followed by mixing and making the volume up to 500ml using sterile water; and
iii. adding 300-500 ml alcohol to step (ii) and making the final volume up to 1 liter with sterile water.

Example 10: Process for preparation of antimicrobial lysis solution
i. Mixing 50ml of Sodium chloride (1M), 50ml of Tris Chloride (1M) and 50ml of EDTA (150mM) (chelating agent) and making the volume up to 400ml with sterile water;
ii. adding 1gm of SDS (detergent) followed by mixing and making the volume up to 500ml using sterile water; and
iii. adding 30ml of Isopropanol (alcohol) to step (ii) and making the final volume up to 1 liter with sterile water.

Example 11: Soaking and packaging of antimicrobial wipe
A 2.5 x 2.5 cm wipe material is impregnated with 200 to 400ul of antimicrobial lysis solution. Each wipe is folded individually for easy use and tightly packed in 100 No’s together in a single box.
,CLAIMS:1. An antimicrobial lysis solution comprising,
a) Salt selected from Sodium chloride or Potassium chloride in an amount of 25 to 100 mM;
b) salt selected from Tris chloride or Ammonium chloride in an amount of 25 to 100 mM;
c) chelating agent selected from ethylene-diamine-tetra-acetic acid (EDTA) or ethylene glycol tetra-acetic acid (EGTA) in an amount of 10mM to 15mM;
d) detergents such as Sodium dodecyl sulphate and Ethyl trimethyl ammonium bromide in an amount of 0.1 to 0.3% and
e) alcohol selected from isopropanol or ethanol in an amount of 30-50%;
wherein said antimicrobial lysis solution wipes out bacterial diversity, eliminates fungal biodiversity and inhibit the colonization of these microbes on gadget for a time period, thereby preventing the transmission of many infectious diseases caused by these microbes from one human being to other by exchange of the gadget.

2. The antimicrobial lysis solution as claimed in claim 1; wherein said lysis solution impregnated on wipe material.

3. The antimicrobial lysis solution as claimed in claim 3; wherein said wipe is made of synthetic analogues of any suitable tissue paper or combination of materials such as non-woven fabric similar to the type used in diapers and dryer sheets made by weaving together fibers of silk, cotton, polyester, wool, and similar materials to form an interlocking matrix of loops or any combination thereof; or made of hydrophilic Spunlace Nonwoven Fabric, PVC synthetic leather non-woven fabric, or Spunlace dependent on any other parameters.

4. The antimicrobial lysis solution as claimed in claim 1; wherein said gadget is selected form smartphone/mobile, tablet, smart watch, laptop or screen of calculator; preferably, a mobile or a tablet.
5. The antimicrobial lysis solution as claimed in claim 1; wherein said time period is about 4-6 days, preferably 4 days.

6. A process for preparation of an antimicrobial lysis solution as claimed in claim 1, wherein said process comprises;
i. Mixing 25-100 ml of Sodium chloride/Potassium chloride (1M), 25-100 ml of Tris chloride/Ammonium chloride (1M) and 50-100 ml of chelating agents (150mM) and making the volume up to 400 ml with sterile water;
ii. adding 1gm - 3gm of detergents followed by mixing and making the volume up to 500 ml using sterile water; and
iii. adding 300-500ml alcohol to step (ii) and making the final volume up to 1 liter with sterile water.

7. A method of lyse bacterial as well as fungal biodiversity and inhibition of colonization of microbes on gadget by applying antimicrobial lysis solution as claimed in claim 1, on the surface of the gadget.

8. A kit comprises wipes impregnated with antimicrobial lysis solution as claimed in claim 1; wherein said wipes are folded and tightly packed individually for easy use.