Some disorders in which free radicals implicated are Alzheimer's disease, arthritis, hemorrhoids, Parkinsonism, rheumatism, heart attack, AIDS, cataract, stroke, cancer and many degenerative diseases including aging. The oxidant/free radicals are species with very short half-life, high reactivity and damaging activity towards macromolecules like protein, DNA and lipid. These species may be either oxygen derived ROS (Reactive oxygen species) or nitrogen derived RNS (reactive nitrogen species). The oxygen derived species include O2" (Superoxide), H02 (Hydroperoxyl), ROO (Peroxyl), RO (Alkoxyl) as free radicals whereas H2O2 (Hydrogen Peroxide), HOC1 (Hypochlorous acid), O3 (Ozone) and O2 (Single oxygen) as non-radical. Similarly, nitrogen derived oxidant species are mainly NO (Nitric oxide), ONOO (Peroxy nitrite), N02 (Nitrogen dioxide) and N2O3 (Dinitrogen trioxide). The exogenous sources of ROS include electromagnetic radiation, cosmic radiation, cigarette smoke, car exhaust, UV light, ozone and low wave length electromagnetic radiation. Similarly, the endogenous sources of ROS are mitochondria electron transport chain, respiratory burst by phagocytes, β-oxidation of fat in peroxisome, auto-oxidation of amino acid, catecholamine's, hemoglobin and ischemia injury. Out of reactive nitrogen species (RNS), nitric oxide is the most important nitrogen derived physiological free radical. It was once considered as a mere toxic pollutant. Later studies reported NO to relax smooth muscles and involved either in the causation or recovery of several diseases, when overall generation of ROS and RNS exceeds the total antioxidants activity in the body, the resulting condition is called oxidative stress.
Antioxidants are the molecules which can safely interact with free radicals and terminate the chain reaction before vital molecules are damaged. These are the substances that, when present at low concentrations compared to those of the oxidizable substrate, significantly delays, or inhibits, oxidation of that substrate. Some antioxidants are proteins and enzymes, while others are small molecules. From the viewpoint of mechanistic functions, the antioxidants may be classified into three types; preventing antioxidants, scavenging antioxidants and repair de novo antioxidants. Antioxidants (also known as free radical scavengers) function

by offering easy electron target for free radicals. In absorbing a free radical, antioxidant "trap" (de-energize or stabilize) the lone free radical electron and make it stable enough to be transported to an enzyme which combines two stabilizes free radicals together to neutralize.
Albizia lebbeck is a deciduous Tree with an open, large, spreading crown; it usually reaches a height of 15 - 20 meters, with exceptional specimens growing up to 30 meters. The straight, cylindrical bole can be 50 - 100cm in diameter, with exceptional specimens. In India plantation-grown siris yields a high quality hardwood that is traded in Europe as 'Indian walnut' or 'koko'. The tree has been widely planted in agro-forestry schemes in the drier parts of the Tropics. Plants are used to provide shade for coffee and cocoa plantations as well as to provide a valuable timber and fuel. It is a popular amenity tree throughout the dry tropics because of its shady spreading habit, although the copious litter produced beneath it is often regarded as a disadvantage. Synonyms:
Acacia lebbec (L.) Willd. Acacia macrophylla Bunge Acacia speciosa (Jacq.) Willd. Taxonomy:
Domain: Eukaryota
Kingdom: Plantae
Phylum: Spermatophyta
Subphylum: Angiospermae
Class: Dicotyledonae
Order: Fabales
Family: Fabaceae
Subfamily: Mimosoideae
Genus: Albizia
Species: Albizia lebbeck (L.) BENTH. Vernacular names:

Arabic : Lebbek,Labakh
Bengali: Sirisha,sirish
English: English woman's tongue, Siris tree, Woman's tongue tree,
Gujarati: Polo barashio
Hindi: Kothia koroi, Kalbaghi
Sanskrit: Sirisha. Morpholgy
Habit: A large tree with a general bluish appearance, unarmed, deciduous, bark pale.
Stem: Erect, cylindrical, branched, solid.
Leaves: Bipinnate, main rachis with a large gland on the petiole near the base and one below the highest pair of pinnae, pinnae 2-4 pairs; leaflets 5-9 pairs. Inflorescence: Heads
Flowers: White, fragrant, heads umbellate, from the upper leaf axils, solitary or fasciculate. Filaments exserted, connate below into a short tube. Fruits: Pods 10 - 25 cm long, 3 - 5 cm broad, thin, flat, straight, straw - coloured when old, glabrous and shining.
Seeds: 4 - 12, spherical or slightly reniform, smooth, glabrous, yellowish brown. Flowering and Fruiting time: July-Oct; Oct-March. Distribution
A. lebbeck is indigenous to the Indian subcontinent and to those areas of South-East Asia with a marked dry season, such as northeastern Thailand, parts of Malaysia and in the eastern islands of Indonesia. This plant is common in Andaman & Nicobar Island, Andhra Pradesh Assam, Kerala, Madhya Pradesh, Maharastra, Meghalaya, Odisha, Rajasthan, Uttar Pradesh. Traditional uses
Albizia lebbeck has many medicinal properties like Antiseptic, antibacterial, anti¬allergic, antidermatosis, antidysenteric etc. Used in the treatment of Bronchitis, piles, hemicranias, cough, tropical pulmonary eosinophilia, asthma etc. Albizia lebbeck is used as an astringent, to treat boils, cough, to treat the eye, flu, gingivitis, lung problems, pectoral problems, to treat abdominal tumors and it is

also used as a tonic. The bark is used medicinally to treat inflammation. Albizia lebbeck is also psychoactive. The wood of Albizia lebbeck is suitable for construction, furniture and veneer. The flower emollient is used as a poultice to be applied to boils. The bark and seed astringent are used against diarrhea, dysentery and hemorrhoids. The seed yields oil used as a cure for leprosy. Albizia lebbeck used in Indian traditional medicine, particularly for asthma and allergic disorders. As a fodder tree, its foliage, twigs, flowers and immature pods are relished by different classes of livestock. This plant is also used as a source of firewood and timber for local peoples.
SILYMARIN is a C25 containing flavonoid mixture, extracted from the Silybum marianum (milk thistle) plant. Today's standardized (according to its silibinin, often called silybin, content) SM extract contains approximately 65% to 80% flavonolignans (silybin A and silybin B, isosilybin A, isosilybin B, silychristin and silydianin), with small amounts of flavonoids, and approximately 20% to 35% of fatty acids and polyphenols compounds possessing a range of metabolic regulatory effects. Silybin was discovered as the first member of a new family of natural compounds called flavonolignans in 1959 and it is known as the predominant and primary active ingredient in SM. That is why compounds containing milk thistle ingredients showing silybin content and silybin antioxidant, as well as other activities in various model systems, are used to explain the biological activity of SM. In particular, SM has been the gold standard drug to treat liver disorders of different etiologies and milk thistle extracts have been used as traditional herbal remedies ("liver tonics") for almost 2000 years. Therefore, SM is most well known for its antioxidant and chemoprotective effects on the liver and it is often prescribed and self-prescribed as a complementary and alternative hepatoprotective medicine [Ref Antioxidants 2015, 4, 204-247; doi:10.3390/antiox4010204].
"(US20150297652)
Compositions containing Albizia lebbeck and/or Sophora flavescens and/or

Hibiscus rosa sinensis and/or Phyllanthus emblica (syn. Emblica officinalis) and/or Azadirachta indica and/or Centella asiatica and/or Ganoderma and/or Rubia cordifolia and/or Scutellaria baicalensis and/or Astragalus membranaceus and/or Ocimum tenuiflorum (syn. Ocimum sanctum) and/or Acorns and/or Artemisia and/or Rheum and/or Schisandra and/or Ophiopogon japonicas and their use in preventing, treating, alleviating symptoms and/or mitigating skin disorders and/or disease are provided." "(US6730332)
The present invention relating to a herbal antiallergic composition which comprises a synergistic mixture of extracts from the fruits of Terminalia chebula, bark of Albizia lebbeck, Terminalia bellerica and Emblica officinalis. The present invention also contains the fruits of Piper longum, Piper nigrum and of rhizomes of Zingiber officinale and thoroughly mixed to get the final composition which has potent antiallergic activity. The invention also relates to a process for the preparation of such composition. The composition is particularly useful for the treatment of allergic conditions." "(WO2006/067802)
The invention provides a novel herbal preparation useful in treatment of allergy. Formulation(s) comprises of plant preparation or active component of plant extract together with conventional additives to form the oral dosage forms, which includes tablets, capsules and liquid dosage forms viz. syrup and suspension as anti-allergic. Formulation(s) comprises of Tinospora cardifolia, Piper longum, Albizia lebbeck and Curcuma amada. Conventionally used as solid or liquid or encapsulated as a soft/hard gelatin capsule for easy consumption as a solid or liquid dosage forms." "(IN4089/CHE/2013)
Medicinal plants are traditionally used in folk medicine as natural healing remedies with therapeutic effects such as prevention of cardiovascular diseases or reducing the risk of cancer. The present study was designated to evaluate the antibacterial and phytochemical activity from the leaves and bark of Albizzia lebbeck. The result of hydroalcohol extracts of leaf showed better antimicrobial

activity followed by the bark extracts. The extracts of Albizzia lebbeck showed better inhibition towards Escherichia coli and Salmonella typhi when compared with Cefoperazone/ Tazobactam. The objective of the present work is to study the invitro antioxidant activity by DPPH scavenging, Nitric oxide assay, hydrogen peroxide, sulphur oxide and total antioxidant assays. The results revealed that the hydroalcoholic leaves extracts of Albizia lebbeck showed a strong antioxidant activity, reducing power ability and free radical scavenging when compared to the standard such as Ascorbic acid. The results on the cytotoxicity tests conducted against human breast adenocarcinoma (MCF-7) and human prostate cancer cell line (PC- 3) showing a certain degree of selectivity against the different cell types."
"(IN1516/MUM/2012)
This invention related to a novel synergistic polyherbal composition comprises of powder or extract of rhizome of Acorus calamus, bark of Albizia lebbeck, fruit of Benincasa hispida, seed of Carthamus tinctorius, aerial part of Euphorbia tirucalli, fruit of Punica granatum, nut of Semecarpus anacardium and leaves of Vitex negundo. It also relates to the method of preparation of the same composition. The said novel composition is useful in the condition of rheumatoid arthritis and other joint related disorder." "(IN2131/DEL/2013)
Shirishadi a polyherbal compound having equal quantity of three herbs namely Albizia lebbeck(L.),Cyperusrottmdus and Solanum xanthocarpum with novel antiplatelet aggregating, mast cell stabilizing, immunomodulatory, bronchodilator, antianaphylactic, antihistaminic and anti-inflammatory properties to prevent and cure allergic respiratory diseases like atopic bronchial asthma and other allergic diseases like allergic rhinitis and urticaria and as stress reliever/ tranquilizer." "(US7344739)
The invention provides a novel herbal preparation useful in treatment of allergy. Formulation(s) comprises of plant preparation or active component of plant extract together with conventional additives to form the oral dosage forms, which includes tablets, capsules and liquid dosage forms viz. syrup and suspension as

anti-allergic. Formulation(s) comprises of Tinospora cardifolia, Piper longum, Albizia lebbeck and Curcuma amada. The formulation can be used as solid or liquid or encapsulated as a soft/hard gelatin capsule for easy consumption as solid or liquid dosage forms." "(IN201641032125)
A novel antioxidant and anti inflammatory herbal composition for lowering blood glucose and cholesterol levels with water extract of herbs. The present invention contains selected herbs such as Terminalia chebula, Phyllanthus emblica, Muraya koeenigi, Tinospora cardifolia, Cyprus rotundus, Syzgium cumini and Phyllanthus amarus. The herbal extract of the present invention is prepared in atleast in one of the forms such as non-encapsulated powdered form, atleast in capsulated form or atleast in tablet form. The said herbal extract is available in dried, powdered form and is administered orally along with water. The herbal composition of the present invention has anti-diabetic, anti-adipogenic, anti-inflammatory and anti-oxidant activity."
The total extract of Albizia lebbeck flowers was examined in vivo for its possible hepatoprotective activity in comparison with the standard drug silymarin at two doses. The higher dose expressed promising activity especially in reducing the levels of AST, ALT and bilirubin. F .[Ref: New acyclic secondary metabolites from the biologically active fraction of Albizia lebbeck flowers. Journal of The Saudi Pharmaceutical Society, (2016), 25, 110]
While the Albizia adianthifolia (Schumach.) W. Wright (Fabaceae) is a traditional herb largely used in the African traditional medicine as analgesic, purgative, antiinflammatory, antioxidant, antimicrobial, memory-enhancer, anxiolytic and antidepressant drug, there are no scientific data that clarify the anxiolytic and antidepressant-like effects in 6-hydroxydopamine (6-OHDA)-lesioned animal model of Parkinson's disease. This study was undertaken in order to identify the effects of aqueous extract of A. adianthifolia leaves on 6-hydroxydopamine-induced anxiety, depression and oxidative stress in the rat amygdala. [Ref: The

aqueous extract of Albizia adianthifolia leaves attenuates 6-hydroxydopamine-induced anxiety, depression and oxidative stress in rat amygdala. BMC Complementary and Alternative Medicine, (2015), 15, 374]
An investigation of the hepatoprotective and antioxidant activities of the chloroform, ethyl acetate and n-butanol fractions of the leaves of Albizia lebbeck L. was performed. The first two fractions expressed the best results regarding the suppression of the increased levels of plasma amino-transferases and alkaline phosphatase in liver-damaged mice (after intoxication with CC14) compared with silymarin and the significantly increased GSH content of alloxan- induced diabetic rats compared with vitamin E (tests and reference drugs were orally administered). [Ref: The phenolic composition of the hepatoprotective and antioxidant fractions of Albizia lebbeck L.. Quimica Nova, (2016), 39, 973]
The total alcoholic extract of Albizia lebbeck, flowers, family Fabaceae showed a highly significant (P< 0.001) protective effect on CC14-induced liver toxicity. Best improvement in biochemical parameters comparable with silymarin was observed in AST and bilirubin levels. [Ref: Hepatoprotective activity and phytochemical study of Albizia lebbeck flowers. Planta Medica, (2015), 81, ]
The aim of this study was to investigate the in-vitro hepatoprotective activity of solvent extracts of Albizia lebbeck, Cassia occidentalis, and Swertia chirata on HepG2 cell line. The silymarin and PCM treated HepG2 cells showed maximum cell viability (156.6±2.49%) presence of leaves extract of S. chirata. The maximum cells viability of 131.6±9.39% was observed in methanolic seed extract of A. lebbeck (50 g/mL), and the minimum cell viability of 107.3±3.68% was observed in acetone seed extract of C. occidentalis (50 g/mL) comparatively. [Ref: In-vitro hepatoprotective activity of Albizia lebbeck, cassia occidentalis and swertia chirata on hepg2 cells. Asian Journal of Pharmaceutical and Clinical Research, (2016), 9, 276]

Thus, it may be noted from the above that antioxitants have immense health benefits and there is a need to prepare a herbal product that is easy to apply and demonstrates potential antioxidant activity. Moreover, the formulation should be easy to apply to the skin with bare hands.
OBJECTS OF THE INVENTION
Some of the objects of the present disclosure, which at least one embodiment herein satisfies, are as follows:
It is an object of the present disclosure to provide an antioxidant composition.
It is still another object of the present disclosure to provide an antioxidant composition comprises extract of Albizia lebbeck and Silymarin.
It is still another object of the present disclosure to provide an antioxidant composition comprises ethanol extract of Albizia lebbeck and Silymarin.
Still another object of the present disclosoure is to provide for a antioxidant composition, the antioxidant potential being measured by DPPH (2,2-diphenyl-l-picrylhydrazyl) inhibition activity.
It is another object of the present disclosure to provide an antioxidant composition comprises ethanol extract of Albizia lebbeck and Silymarin in HPMC gel or film form is applied on the skin for antioxidant effect.
It is yet another object of the present disclosure to provide an antioxidant composition comprises ethanol extract of Albizia lebbeck and Silymarin in HPMC gel or film form is applied on the skin for antioxidant effect with bare hands.

SUMMARY OF THE INVENTION
The following presents a simplified summary of the invention in order to provide
a basic understanding of some aspects of the invention. This summary is not an
extensive overview of the present invention. It is not intended to identify the
key/critical elements of the invention or to delineate the scope of the invention. Its
sole purpose is to present some concept of the invention in a simplified form as a
prelude to a more detailed description of the invention presented later.
In a preferred embodiment of the present invention is an antioxidant composition.
In another embodiment of the present invention the antioxidant composition
comprises extract of Albizia lebbeck.
In another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck.
In still another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck.
In still another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck and Silymarin.
In still another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck and Silymarin show synergestic
action.
In still another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck and Silymarin in gel form.
In still another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck and Silymarin in film form.
In still another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck and Silymarin in HPMC gel form.
In still another embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck and Silymarin in HPMC film form.
In still embodiment of the present invention the antioxidant composition
comprises ethanol extract of Albizia lebbeck and Silymarin in HPMC gel or film
form is applied on the skin.
In yet embodiment of the present invention the antioxidant composition

The present disclosure is further described in light of the following experiments which are set forth for illustration purpose only and not to be construed for limiting the scope of the disclosure. The following experiments can be scaled up to industrial/commercial scale and the results obtained can be extrapolated to industrial scale.
EXAMPLES
EXAMPLE 1: Antioxidant activity DPPH (2,2-diphenyl-l-picrylhydrazyl)
Assay
The method is based on the scavenging of DPPH by antioxidants, which upon a reduction reaction decolorizes the DPPH methanol solution. The assay measures the reducing ability of antioxidants toward the DPPH radical.
200 l of the test sample solution was added to 4ml of 100 mM methanolic
DPPH. The sample tubes were wrapped with aluminum foil and kept in dark for
30 min at room temperature and the absorbance was measured at 517nm using
UV-Vis spectrophotometer with solvent and DPPH as blank. Ascorbic acid (100
mg/ml) was used as reference. The percentage inhibition of DPPH activity was
estimated using the formula:
Acontrol — Asample
Percent inhibition of DPPH = X 100
Acontrol
where Acontrol is the absorbance of DPPH in methanol without an antioxidant and Asample is the absorbance of DPPH in the presence of an antioxidant
The antioxidant activity data of the selected test compounds are listed in Table 1. The DPPH radical was scavenged by antioxidants via donation of hydrogen resulting in the formation of DPPH. The colour of the DPPH changed from purple to yellow after reduction, which was quantified by the decline of absorbance at a wavelength of 517 nm. Among the tested compounds, the combination showed

comprises ethanol extract of Albizia lebbeck and Silymarin in HPMC gel or film form is applied on the skin for antioxidant effect.
DETAILED DESCRIPTION OF THE INVENTION
The following description is of exemplary embodiments only and is not intended to limit the scope, applicability or configuration of the invention in any way. Rather, the following description provides a convenient illustration for implementing exemplary embodiments of the invention. Various changes to the described embodiments may be made in the function and arrangement of the elements described without departing from the scope of the invention.
The present inventors have surprisingly found that antioxidant composition comprises ethanol extract of leaves of Albizia lebbeck and Silymarin ((2R,3R)-3,5,7-trihydroxy- 2-[(2R,3R)-3-(4-hydroxy-3-methoxyphenyl)-2-(hydroxymethyl) -2,3-dihydrobenzo[b][l,4]dioxin-6-yl]chroman-4-one) show synergestic action better than ascorbic acid, the gold stadard for antioxidant effect.
HPMC (hydroxypropyl methylcellulose) 4% w/v was added in distilled water containing ethanolic extract of Albizia lebbeck (50mg/ml) and Silymarin (50mg/ml) and allowed to hydrate overnight to form the gel.
The gel was mixed with humidifier like glycerin to impart flexibility and was dried under vacuum to obtain the film.
HPMC acts as a gelling and film forming agent, other gelling and film forming agents may also be employed.
Further, the composition is capable of being applied to the skin in the form of gel or film with bare hands.
Source: Albizia lebbeck was procured from Moradabad, Uttar Pradesh, India

synergistic antioxidant activity better than ascorbic acid (Table 1).

While considerable emphasis has been placed herein on the specific features of the preferred embodiment, it will be appreciated that many additional features can be added and that many changes can be made in the preferred embodiment without departing from the principles of the disclosure. These and other changes in the preferred embodiment of the disclosure will be apparent to those skilled in the art from the disclosure herein, whereby it is to be distinctly understood that the foregoing descriptive matter is to be interpreted merely as illustrative of the disclosure and not as a limitation.

I Claim,

1. An antioxidant composition comprising extract Albizia lebbeck and silymarin.
2. The antioxidant composition according to claim 1, wherein the extract Albizia lebbeck and silymarin are in used in equal proportion..
3. The antioxidant composition according to claim 1, wherein the extract Albizia lebbeck and silymarin are dissolved in water and mixed with gelling agent.
4. The antioxidant composition according to claim 1, wherein the extract Albizia lebbeck and silymarin are dissolved in water and mixed with film forming agent.
5. The antioxidant composition according to claim 4, wherein the extract Albizia lebbeck and silymarin are dissolved in water and mixed with HPMC (hydroxyl popyl methyl cellulose) to form gel.
6. The antioxidant composition according to claim 5, wherein the extract Albizia lebbeck and silymarin are dissolved in water and mixed with HPMC (hydroxyl popyl methyl cellulose) to form film..
7. The antioxidant composition according to claim 4, wherein the extract Albizia lebbeck and silymarin are dissolved in water and mixed with HPMC (hydroxyl popyl methyl cellulose) to form gel for being applied to the skin.
8. The antioxidant composition according to claim 5, wherein the extract Albizia lebbeck and silymarin are dissolved in water and mixed with HPMC (hydroxyl popyl methyl cellulose) to form film for being applied to the skin.