FIELD OF INVENTION
[001] The present disclosure broadly relates to field of food formulations and
particularly refers to food composition exhibiting antioxidant activity.
5 BACKGROUND OF INVENTION
[002] Process of metabolism displayed by a living cell causes degeneration of cell due to the oxidant nature of the by-products. Metabolism causes oxidative damage to bio-molecules like nucleic acid, proteins, and lipids. It is roughly estimated that the extent of damage is roughly proportional to metabolic rate in a number of
10 mammalian species, which is the major contributor to functional degeneration of somatic cells causing aging and degenerative diseases such as cancer, cardiovascular disease, immune-system decline, brain dysfunction, and cataracts. (Oxidants, antioxidants, and the degenerative diseases of aging. Bruce N. Ames*, Mark K. Shigenaga, and Tory M. Hagen, Proc Natl. Acad. Sci. USA, Vol. 90, pp. 7915-7922,
15 September 1993)
[003] Antioxidant is a group of nutrients, which have protective effects against oxidative damage to the cell. In humans, there exists appropriate defence mechanisms which limit the levels of reactive oxidants and the damage they inflict. Foremost is the defence mechanism of endogenous enzymes such as superoxide
20 dismutase, catalase, and glutathione peroxidase.
[004] Considerable amount of work has been done on the sources, use and characteristics of antioxidant compounds. Majority of the work covered the antioxidant properties of flavonoids, vitamins, and synthetic chemicals. Achievement of positive results has led to rampant use of antioxidants in food
25 formulations as well as in other topical applications. Apart from providing health benefits, the presence of anti-oxidants also preserves the nutrients in the food formulations. Widely available food formulations are generally made up of natural or synthetic antioxidants. Since the use of synthetic antioxidants like butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and propyl gallate has
30 been associated with detrimental effects, appropriate alternatives are being studied.
2

[005] US20050184275 discloses an antioxidant composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition is operable to inhibit oxidation of lipids in oil-in-water or water-in-oil emulsions. [006] WO2002081027 discloses an antioxidant composition comprising 5 carotenoids, polyphenols, vitamins, and helioxine. The compositions according to the invention have an improved effect on protecting the skin against damages to the cells and skin caused by free radicals of oxygen.
SUMMARY OF THE INVENTION
[007] In an aspect of the present disclosure, there is provided an edible antioxidant
10 composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1.
[008] In an aspect of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe
15 vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i) obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice; (iv) obtaining Indian gooseberry juice; and (v) contacting the green apple juice, the carrot juice, the Aloe vera juice, and the Indian gooseberry juice, to obtain the
20 composition.
[009] These and other features, aspects, and advantages of the present subject matter will be better understood with reference to the following description and appended claims. This summary is provided to introduce a selection of concepts in a simplified form. This summary is not intended to identify key features or essential
25 features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter.
3

BRIEF DESCRIPTION OF ACCOMPANYING DRAWINGS
[0010] The following drawings form a part of the present specification and are included to further illustrate aspects of the present disclosure. The disclosure may be better understood by reference to the drawings in combination with the detailed description of the specific embodiments presented herein. 5 [0011] Figure 1 depicts antioxidant activity of varying concentrations of green apple juice, carrot juice, India gooseberry juice, and Aloe vera juice, in accordance with an embodiment of the present disclosure.
[0012] Figure 2 depicts various combinations of four components - green apple juice, carrot juice, India gooseberry juice, and Aloe vera juice of the composition, in 10 accordance with an embodiment of the present disclosure ‘WE’ refers to Working Example and ‘NWE’ refers to Non-Working Example, the number accompanying the abbreviations refer to the respective number of Example as per the respective Table in the ‘Examples’ section.
15 DETAILED DESCRIPTION OF THE INVENTION
[0013] Those skilled in the art will be aware that the present disclosure is subject to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The disclosure also includes all such steps, features, compositions, and compounds
20 referred to or indicated in this specification, individually or collectively, and any and all combinations of any or more of such steps or features. Definitions
[0014] For convenience, before further description of the present disclosure, certain terms employed in the specification, and examples are delineated here. These
25 definitions should be read in the light of the remainder of the disclosure and understood as by a person of skill in the art. The terms used herein have the meanings recognized and known to those of skill in the art, however, for convenience and completeness, particular terms and their meanings are set forth below.
4

[0015] The articles “a”, “an” and “the” are used to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article.
[0016] The terms “comprise” and “comprising” are used in the inclusive, open sense, meaning that additional elements may be included. It is not intended to be construed 5 as “consists of only”.
[0017] Throughout this specification, unless the context requires otherwise the word “comprise”, and variations such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated element or step or group of element or steps but not the exclusion of any other element or step or group of element or steps.
10 [0018] The term “including” is used to mean “including but not limited to”. “Including” and “including but not limited to” are used interchangeably. [0019] For the purposes of the present document, the term “juice” used along with different sources refer to the commercially procured juice. For example, green apple juice refers to the commercial source of green apple juice and the like.
15 [0020] Ratios, concentrations, amounts, and other numerical data may be presented herein in a range format. It is to be understood that such range format is used merely for convenience and brevity and should be interpreted flexibly to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as
20 if each numerical value and sub-range is explicitly recited.
[0021] To exploit the benefits of antioxidant compositions, and to simultaneously minimize the risk posed by synthetic antioxidants, naturally occurring dietary antioxidants seem to be of great importance in the body as they scavenge harmful free radicals, which are implicated in most degenerative diseases. The antioxidant
25 content of fruits and vegetables is a major contributor of a lowered risk of degenerative diseases and promotion of good health. Epidemiological studies have established a positive correlation between the intake of fruits and vegetables and prevention of diseases like atherosclerosis, cancer, diabetes, arthritis and also aging. [0022] Fruits and vegetables are considered to be rich in nutrients such as fibre,
30 vitamins and minerals, phytochemicals that function as antioxidants, phytoestrogens, and anti-inflammatory agents and provide protective mechanisms. (Joanne L. Slavin
5

, Beate Lloyd , Health Benefits of Fruits and Vegetables, Advances in Nutrition, Volume 3, Issue 4, 1 July 2012, Pages 506–516). In view of the benefits posed by the natural antioxidants, the present disclosure provides an antioxidant-rich composition comprising juices from four different sources. Juices from green apple, 5 carrot, Aloe vera, and Indian gooseberry has been exploited in the composition of the present disclosure.
[0023] Aleo vera Juice: Aloe vera is known for its bioactive compounds that possess pharmacological and phytochemical properties. The plant contains many vitamins, including the important antioxidant vitamins A, C and F, vitamin B (thiamine),
10 niacin, vitamin B2 (riboflavin), vitamin B12, choline and folic acid. When taken internally, Aloe vera juice aids the digestion and absorption of nutrients, helps control blood sugar by decreasing insulin resistance, increases energy production, promotes cardiovascular health, improves liver function, and boosts the immune system. The pulp is used extensively in Siddha medicines, for balancing digestion
15 and elimination, for treating constipation. Aloe helps clear the toxins out of the digestive system, facilitates digestion and improves the functioning of the kidneys, liver and gall bladder. The anti-inflammatory fatty acids in Aloe vera alkalize the digestive juices and prevent acidity-a common cause of indigestion. These fatty acids benefit not only the stomach but the small intestine and the colon as well. Aloe vera
20 is known for antiseptic, antimicrobial and anti-inflammation. (Baby Joseph, and S.Justin Raj. International Journal of Pharmaceutical Sciences Review and Research, Volume 4, Issue 2, September – October 2010; Article 017.)
[0024] Indian gooseberry Juice or Amla: Emblica officinalis or Phyllanthus emblica (Indian Goose Berry scientific name). It is the richest natural source of vitamin,
25 especially heat stable Vitamin C, which is known for antioxidant property. It is very effective in treatment of acidity, peptic ulcers and is an excellent in anti-aging. Regular intake of Amla with other herbs or alone improves immunity, and anaemia, rejuvenates the body and fights chronic diseases like hypertension, high cholesterol, diabetes, AIDS, influenza, chronic cough, cold, infections, fatigue and inflammatory
30 conditions. (K.P. Sampath Kumar et al. Journal of Pharmacognosy and Phytochemistry, ISSN 2278- 4136)
6

[0025] Green Apple: Apples, Malus pumila do contain bioavailable phytochemicals (quercetin, catechin, phloridzin and chlorogenic acid), all of which have been found to have strong antioxidant activity and anticancer activity. Several epidemiological studies have shown that consumption of apples reduces the risk of some cancers, 5 cardiovascular disease, asthma, and diabetes (Jeanelle Boyer and Rui Hai Liu, Apple phytochemicals and their health benefits, Nutrition Journal 2004; Dec;3(1):5) [0026] Carrot: Carrot, Daucus carota is of nutritional and health benefits as it contains carotenoids, flavonoids, polyacetylenes, vitamins, and minerals which act as antioxidants neutralizing the effect of free radicals. Carrot is a good source of
10 dietary fiber and of the trace mineral molybdenum and manganese, rarely found in many vegetables. Carrots are found to beneficial in being anti-diabetic, cholesterol and cardiovascular disease lowering, anti-hypertensive, hepatoprotective, renoprotective, wound healing, reduce inflammatory insult and modulate immune response. (João Carlos da Silva Dias, Nutritional and Health Benefits of Carrots and
15 Their Seed Extracts, Food and Nutrition Sciences, Vol.05 No.22(2014), Article ID:52066,9 pages).
[0027] The juices as used in the present disclosure were procured from a commercial source. The present disclosure pertains to the synergistic composition achieved through the use of the juices as mentioned herein. It has also been provided that if
20 the juice composition is made of the individual juices outside a specific weight range, it is not displaying desirable synergistic antioxidant effect.
[0028] The present disclosure discloses an antioxidant-rich composition comprising green apple juice, carrot juice, Aloe vera juice, and Indian gooseberry juice. The four different juices when present in specific weight ratios as disclosed in the present
25 disclosure provides a synergistic antioxidant effect.
[0029] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the
30 disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference.
7

[0030] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods are clearly within the scope of the disclosure, as described herein. 5 [0031] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1. In another embodiment of the present disclosure, the green apple juice to the carrot juice has a weight ratio in a
10 range of 1:1 to 5:1.
[0032] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the Aloe vera juice to
15 the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 40:1. In another embodiment of the present disclosure, the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 35:1.
[0033] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe
20 vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2. In another embodiment of the present disclosure, the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 65:1 to 1:2.
25 [0034] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 40:1, and wherein
30 the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2. In another embodiment of the present disclosure, the green apple juice
8

to the carrot juice has a weight ratio in a range of 1:1 to 5:1, and wherein the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 35:1, and wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 65:1 to 1:2. 5 [0035] In an embodiment of the present disclosure, there is provided an edible antioxidant composition as described herein, wherein the green apple juice has a total phenolic concentration in a range of 10-20mg/ml of the green apple juice, the carrot juice has a total phenolic concentration in a range of 2.5-7.5mg/ml of the carrot juice, the Aloe vera juice has a total phenolic concentration in a range of 3-10mg/ml of the
10 Aloe vera juice, and the Indian gooseberry juice has a total phenolic concentration in a range of 60-70mg/ml of the Indian gooseberry juice.
[0036] In an embodiment of the present disclosure, there is provided an edible antioxidant composition as described herein, wherein the composition comprises: (a) the green apple juice having a weight percentage in a range of 1.0-8% with respect
15 to the composition; (b) the carrot juice having a weight percentage in a range of 0.75-4.5% with respect to the composition; (c) the Aloe vera juice having a weight percentage in a range of 0.4-4.5% with respect to the composition; and (d) the Indian gooseberry juice having a weight percentage in a range of 0.05-0.5% with respect to the composition. In another embodiment of the present disclosure, the green apple
20 juice has a weight percentage in a range of 1.0-6.5% with respect to the composition, the carrot juice has a weight percentage in a range of 1.0-3.5% with respect to the composition, the Aloe vera juice has a weight percentage in a range of 0.6-3.5% with respect to the composition, and the Indian gooseberry juice has a weight percentage in a range of 0.08-0.3% with respect to the composition.
25 [0037] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the green apple juice has a weight percentage in a range of 1.0-8% with respect to the composition, the
30 carrot juice has a weight percentage in a range of 0.75-4.5% with respect to the composition, the Aloe vera juice has a weight percentage in a range of 0.4-4.5% with
9

respect to the composition, and the Indian gooseberry juice has a weight percentage in a range of 0.05-0.5% with respect to the composition.
[0038] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe 5 vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1. [0039] In an embodiment of the present disclosure, there is provided an edible
10 antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the
15 Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 40:1.
[0040] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a
20 group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2.
25 [0041] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple
30 juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1
10

to 40:1, and wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2.
[0042] In an embodiment of the present disclosure, there is provided an edible antioxidant composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe 5 vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, and wherein the green apple juice has a weight percentage in a range of 1.0-8% with respect to the
10 composition, the carrot juice has a weight percentage in a range of 0.75-4.5% with respect to the composition, the Aloe vera juice has a weight percentage in a range of 0.4-4.5% with respect to the composition, and the Indian gooseberry juice has a weight percentage in a range of 0.05-0.5% with respect to the composition. In another embodiment of the present disclosure, the green apple juice has a weight
15 percentage in a range of 1.0-6.5% with respect to the composition, the carrot juice has a weight percentage in a range of 1.0-3.5% with respect to the composition, the Aloe vera juice has a weight percentage in a range of 0.6-3.5% with respect to the composition, and the Indian gooseberry juice has a weight percentage in a range of 0.08-0.3% with respect to the composition.
20 [0043] In an embodiment of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i) obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice;
25 (iv) obtaining Indian gooseberry juice; and (v) contacting the green apple juice, the carrot juice, the Aloe vera juice, and the Indian gooseberry juice, to obtain the composition.
[0044] In an embodiment of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe
30 vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i)
11

obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice; (iv) obtaining Indian gooseberry juice; and (v) contacting the green apple juice, the carrot juice, the Aloe vera juice, and the Indian gooseberry juice, to obtain the composition, and wherein the Aloe vera juice to the Indian gooseberry juice has a 5 weight ratio in a range of 1.1:1 to 40:1.
[0045] In an embodiment of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i)
10 obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice; (iv) obtaining Indian gooseberry juice; and (v) contacting the green apple juice, the carrot juice, the Aloe vera juice, and the Indian gooseberry juice, to obtain the composition, and wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2.
15 [0046] In an embodiment of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; and (d) Indian gooseberry juice, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i) obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice;
20 (iv) obtaining Indian gooseberry juice; and (v) contacting the green apple juice, the carrot juice, the Aloe vera juice, and the Indian gooseberry juice, to obtain the composition, and wherein the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 40:1, and the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2.
25 [0047] In an embodiment of the present disclosure, there is provided a process as described herein, wherein the green apple juice has a weight percentage in a range of 1.0-8% with respect to the composition, the carrot juice has a weight percentage in a range of 0.75-4.5% with respect to the composition, the Aloe vera juice has a weight percentage in a range of 0.4-4.5% with respect to the composition, and the
30 Indian gooseberry juice has a weight percentage in a range of 0.05-0.5% with respect to the composition. In another embodiment of the present disclosure, the green apple
12

juice has a weight percentage in a range of 1.0-6.5% with respect to the composition, the carrot juice has a weight percentage in a range of 1.0-3.5% with respect to the composition, the Aloe vera juice has a weight percentage in a range of 0.6-3.5% with respect to the composition, and the Indian gooseberry juice has a weight percentage 5 in a range of 0.08-0.3% with respect to the composition.
[0048] In an embodiment of the present disclosure, there is provided a process as described herein, wherein the green apple juice has a total phenolic concentration in a range of 10-20mg/ml of the green apple juice, and the carrot juice has a total phenolic concentration in a range of 2.5-7.5mg/ml of the carrot juice, and the Aloe
10 vera juice has a total phenolic concentration in a range of 3-10mg/ml of the Aloe vera juice, and the Indian gooseberry juice has a total phenolic concentration in a range of 60-70mg/ml of the Indian gooseberry juice.
[0049] In an embodiment of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe
15 vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i) obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining
20 Aloe vera juice; (iv) obtaining Indian gooseberry juice; (v) obtaining the at least one excipient; and (vi) contacting the green apple juice, the carrot juice, the Aloe vera juice, the Indian gooseberry juice, and the at least one excipient, to obtain the composition. [0050] In an embodiment of the present disclosure, there is provided a process for
25 preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process
30 comprising: (i) obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice; (iv) obtaining Indian gooseberry juice; (v) obtaining the at least one
13

excipient; and (vi) contacting the green apple juice, the carrot juice, the Aloe vera juice, the Indian gooseberry juice, and the at least one excipient, to obtain the composition, and wherein the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 40:1. 5 [0051] In an embodiment of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple
10 juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i) obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice; (iv) obtaining Indian gooseberry juice; (v) obtaining the at least one excipient; and (vi) contacting the green apple juice, the carrot juice, the Aloe vera juice, the Indian gooseberry juice, and the at least one excipient, to obtain the
15 composition, and wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2.
[0052] In an embodiment of the present disclosure, there is provided a process for preparing the composition comprising: (a) green apple juice; (b) carrot juice; (c) Aloe vera juice; (d) Indian gooseberry juice; and (e) at least one excipient selected from a
20 group consisting of beetroot juice, pepper powder, ginger powder, salt, stabilizer, preservative, acidity regulator, and combinations thereof, wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1, said process comprising: (i) obtaining green apple juice; (ii) obtaining carrot juice; (iii) obtaining Aloe vera juice; (iv) obtaining Indian gooseberry juice; (v) obtaining the at least one
25 excipient; and (vi) contacting the green apple juice, the carrot juice, the Aloe vera juice, the Indian gooseberry juice, and the at least one excipient, to obtain the composition, and wherein the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 40:1, and wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2.
14

[0053] In an embodiment of the present disclosure, there is provided a composition as described herein, wherein the composition is an antioxidant-rich juice composition.
[0054] In an embodiment of the present disclosure, there is provided a composition 5 as described herein, wherein the composition is in form of a dry lyophilized powder. [0055] Although the subject matter has been described in considerable detail with reference to certain examples and implementations thereof, other implementations are possible.
10 EXAMPLES
[0056] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as
15 commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions
20 described, as such methods and conditions may apply.
The working and non-working examples as depicted in the forthcoming sections highlight the criticality of the working percentages of different juice components in achieving the anti-oxidant rich composition of the present disclosure. It is further specified that the presence of green apple juice, carrot juice, Aloe vera juice, and
25 Indian gooseberry juice is critical so as to achieve syngeristic antioxidant activity. The absence of any of the fruit juice specified above or replacement of the same with any other juice substantially affects the synergistic antioxidant effect.
Materials and Methods
30 [0057] The juice components – green apple juice, carrot juice, Indian gooseberry juice, and Aloe vera juice used for arriving at the composition of the present
15

disclosure were commercially procured. Also, beetroot juice, ginger powder and pepper used as an additional ingredients in the antioxidant-rich edible composition of the present disclosure was procured commercially.
[0058] FRAP assay - The assay refers to Ferric reducing antioxidant power assay. 5 Commercially procured kit was used to perform the assay. For this purpose, to 24]il of test sample 180jil of FRAP reagent was added. Allowed to incubate for 10 minutes. Absorbance was read at 593nm.
[0059] Standard curve was plotted using FeS04.7H20. FRAP reagent was prepared using Sodium acetate buffer, TPTZ-Sigma Aldrich (lOmM/L) and FeCh-Merck 10 (20mM/L) in the ratio 10:1:1.
Example 1
Working concentrations of the juice components and antioxidant effect
displayed by individual components 15 [0060] The FRAP assay was performed with different weight percentages of the four
juice components. The assay was done with 3.13%, 6.25%, 12.50%, 25%, 50%, and
100% for green apple juice and carrotjuice independently. Various percentages used
for Indian gooseberry juice were 0.09%, 0.19%, 0.39%, 0.78%, 1.56%, 3.13%,
6.25%, and 12.50%. In case of Aloe verajuice, 0.39%, 0.78%, 1.56%, 3.13%, 6.25%, 20 and 12.50% were tested. The results obtained have been depicted in Figure 1.
[0061] Assessing the data in Figure 1, stock concentration was derived for each
component as depicted in Table 1 below.
Table 1:

[0062] It was established that using further concentrated versions of the juice components interfered with FRAP assay and did not provide desirable observations with respect to the antioxidant effects. The commercially procured juice components were used in the above-mentioned weight percentages (Table 1) to arrive at the final 5 composition comprising all the four components.
Example 2
Working and non-working example of the composition of the present disclosure
[0063] Process of preparation: The antioxidant-rich composition of the present
10 disclosure was obtained by mixing green apple juice, carrot juice, Indian gooseberry juice, and Aloe vera juice in specific weight percentages and ratios as described in the present disclosure. The mixing is not to be restricted with any particular parameters as it is done to ensure proper distribution of all the contents in the final mixture. Also, same process was followed to prepare various test compositions
15 including the non-working compositions.
[0064] Different types of juices were defined by the presence of total phenolic concentration in the respective juices. The green apple juice has a total phenolic concentration in a range of 10-20mg/ml of the green apple juice, the carrot juice has a total phenolic concentration in a range of 2.5-7.5mg/ml of the carrot juice, the Aloe
20 vera juice has a total phenolic concentration in a range of 3-10mg/ml of the Aloe vera juice, and the Indian gooseberry juice has a total phenolic concentration in a range of 60-70mg/ml of the Indian gooseberry juice.
[0065] FRAP assay was performed to assess the antioxidant potential of various test compositions. Table 2 depicts different compositions of the present disclosure
25 which are showing synergistic antioxidant effect. Table 2:

[0066] Referring to Table 2, the column depicting theoretical data is the additive effect of individual antioxidant potential of the four components at respective weight percentages specific to particular compositions. In case of Working Example 1: 5 respective components mentioned in the synergistic ratio, for example the additive value of 6.25% of green apple juice, 1.56% of carrot juice, 0.097% of Indian gooseberry juice, and 1.56% of Aloe vera juice independently, is lesser than the antioxidant effect of the composition 1 (individual data depicted in Figure 1 and combinatorial data depicted in Figure 2). Therefore, it can be inferred that Example 10 1 is displaying a synergistic antioxidant effect. Likewise, synergistic antioxidant effect can be observed in the examples B to F.
[0067] Table 3 depicts the Working Examples (WE) 1 to 6 depicting the individual weight percentages (wt. %). The Examples 1-6 have also been depicted in Figure 2. Table 3:

[0068] Table 4 depicts the Non-Working Examples (NWE) 8 to 13 with respect to the deviation in weight percentages (wt. %) of the four components. The Non-Working Examples 8, 9, 12, and 13 have been depicted in Figure 2 as not showing a 5 distinct synergistic effect. Table 4:
[0069] Referring to Table 4, it can be observed that Non-Working Examples 8-10, 12, and 13 are not showing synergistic effect as weight percentage of carrot juice is 10 higher than green apple juice.
[0070] Table 5 depicts Non-Working Examples 14-20 Table 5:

[0071] Referring to Table 5, it can be observed that Non-Working Examples 14-20 are not showing synergistic antioxidant effect because of lack of one of the components (Aloe vera juice). 5 [0072] Table 6 depicts Non-Working Examples 21-28 Table 6:
[0073] As depicted in Table 6, the absence of the Indian gooseberry juice in the composition did not result in the enhancement of the antioxidant effect.
20

[0074] Table 7 depicts Non-Working Example 29-35. Table 7:
[0075] As depicted in Table 7, the absence of green apple juice makes the above-5 depicted examples as Non-Working since they do not exhibit synergistic antioxidant effect.
[0076] Table 8 depicts Non-Working Examples 36-42 Table 8:

[0077] As depicted in Table 8, the absence of carrot juice makes the above-depicted examples as Non-Working since they do not exhibit synergistic antioxidant effect. [0078] Table 9 depicts Non-Working Examples 43-47. 5 Table 9:
[0079] As depicted in Table 9, the absence of both Indian gooseberry and Aloe vera juice makes the above-depicted examples as Non-Working since they do not exhibit synergistic antioxidant effect. 10 [0080] Table 10 depicts Non-Working Examples 48-52. Table 10:

[0081] As depicted in Table 10, the absence of both carrot and Aloe vera juice makes the above-depicted examples as Non-Working since they do not exhibit synergistic antioxidant effect. 5 [0082] Table 11 depicts the Non-Working Examples 53-57. Table 11:
[0083] As depicted in Table 11, the absence of both green apple and Aloe vera juice makes the above-depicted examples as Non-Working since they do not exhibit 10 synergistic antioxidant effect.
[0084] Table 12 depicts the Non-Working Examples 58-62. Table 12:

[0085] As depicted in Table 12, the absence of both green apple and Indian gooseberry juice makes the above-depicted examples as Non-Working since they do not exhibit synergistic antioxidant effect. 5 [0086] Table 13 depicts the Non-Working Examples 63-67. Table 13:
[0087] As depicted in Table 13, the absence of both carrot and Indian gooseberry juice makes the above-depicted examples as Non-Working since they do not exhibit 10 synergistic antioxidant effect.
[0088] Table 14 depicts the Non-Working Examples 68-72. Table 14:
24

[0089] As depicted in Table 14, the absence of both carrot and green apple juice makes the above-depicted examples as Non-Working since they do not exhibit synergistic antioxidant effect. 5 [0090] Table 15 depicts the Non-Working Examples 73-76. Table 15:
[0091] The Non-Working Examples depicted in the above table uses radish juice
instead of carrot juice. As can be observed none of the Non-Working Examples
10 mentioned in the Table are showing synergistic antioxidant effect. Therefore, it can
25

be appreciated that replacing any one component with any other does not yield
desirable effect.
[0092] Table 16 depict Non-Working Examples 77-80.
Table 16
[0093] The Non-Working Examples depicted in the above table uses lemon juice instead of Indian gooseberry juice, as can be observed none of the Non-Working Examples mentioned in the table are showing synergistic antioxidant effect. Therefore, it can be appreciated that replacing any one component with any other 10 does not yield desirable effect.
[0094] Table 17 depict Non-Working Examples 81-84. Table 17

[0095] The Non-Working Examples depicted in the above table uses cucumber
juice instead of Aloe vera juice, as can be observed none of the Non-Working
Examples mentioned in the table are showing synergistic antioxidant effect.
Therefore, it can be appreciated that replacing any one component with any other
does not yield desirable effect.
[0096] Table 18 depict Non-Working Examples 85-88.
Table 18:
10 [0097] The Non-Working Examples depicted in the above table uses red apple juice instead of green apple juice, as can be observed none of the Non-Working Examples mentioned in the table are showing synergistic antioxidant effect. Therefore, it can be appreciated that replacing any one component with any other does not yield desirable effect.
15
Example 3
27

Formulation (Antioxidant rich juice) comprising the composition of the present disclosure
[0098] Table 19 depicts a formulation table comprising the composition of the present disclosure. The formulation has been prepared by mixing the relevant 5 components in the respective weight percentages. Beetroot juice, pepper, ginger powder, and salt have been used as excipients. The formulation as depicted is a mixed juice formulation providing a synergistic antioxidant effect. The beetroot juice used in the present formulation does not contribute to the antioxidant activity of the final composition and as per desired sensorial it can be effectively replaced with any 10 other component which does not affect the antioxidant activity of the formulation. Table 19:
[0099] Although the end-product is a liquid formulation, but it can be contemplated that the end-product can further be converted to dry powder form by lyophilization
15 or any other well-known technique in the art.
[00100] Overall, the combination of green apple juice, carrot juice, Aloe vera juice, and Indian gooseberry juice in the disclosed weight ranges is essential to have a desired antioxidant effect. Replacing even a single component with another component like lemon juice, red apple juice, or radish juice does not provide the
20 desired effect. Also, deviating from the disclosed weight ranges does not exhibit the desired result.
28

Advantages of the present disclosure
[00101] The present disclosure discloses an antioxidant-rich composition comprising green apple juice, carrot juice, Aloe vera juice, and Indian gooseberry juice as per the disclosed weight percentage ranges. The antioxidant-rich 5 composition provides a synergistic antioxidant effect. Being formed from natural sources, the composition is quite safe to consume and can exhibit the antioxidant effect in natural ways. Apart from providing antioxidant effect, the composition can provide an overall health-benefitting effect. The raw materials required for the preparation of the composition are abundantly available and the process of 10 preparation of the composition is also quite simple and rapid.
29

I/We Claim:
1. An edible antioxidant composition comprising:
a) green apple juice;
b) carrot juice;
5 c) Aloe vera juice; and
d) Indian gooseberry juice,
wherein the green apple juice to the carrot juice has a weight ratio in a range of 1:1 to 10:1.
2. The composition as claimed in claim 1, wherein the green apple juice to the
10 carrot juice has a weight ratio in a range of 1:1 to 5:1.
3. The composition as claimed in claim 1, wherein the Aloe vera juice to the Indian gooseberry juice has a weight ratio in a range of 1.1:1 to 40:1.
4. The composition as claimed in claim 1, wherein the green apple juice to the Indian gooseberry juice has a weight ratio in a range of 70:1 to 1:2.
15 5. The composition as claimed in claim 1, wherein the green apple juice has a
total phenolic concentration in a range of 10-20mg/ml of the green apple juice, and the carrot juice has a total phenolic concentration in a range of 2.5-7.5mg/ml of the carrot juice, and the Aloe vera juice has a total phenolic concentration in a range of 3-10mg/ml of the Aloe vera juice, and the Indian
20 gooseberry juice has a total phenolic concentration in a range of 60-70mg/ml
of the Indian gooseberry juice.
6. The composition as claimed in claim 1, wherein the composition comprises:
(a) the green apple juice having a weight percentage in a range of 1.0-8%
with respect to the composition; (b) the carrot juice having a weight
25 percentage in a range of 0.75-4.5% with respect to the composition; (c) the
Aloe vera juice having a weight percentage in a range of 0.4-4.5% with respect to the composition; and (d) the Indian gooseberry juice having a weight percentage in a range of 0.05-0.5% with respect to the composition.
7. The composition as claimed in claim 1, wherein the composition further
30 comprises at least one excipient selected from a group consisting of beetroot
30