FIELD OF INVENTION
Apparatus and method for the housing of toothbrush for prevention and destruction of microbial load deposited onto the bristle and surrounding area of used toothbrushes by novel kit for oral hygiene.
10001] Technical Field:
The toothbrush can serve as a reservor for microorganisms.Researchers has found more than 10.000,000 bacteria living on a single toothbrush. Tooth decay is caused by bacteria that can survive on toothbrushes. Viruses that cause colds, flu, and fever blisters can survive for many days on toothbrushes.Influenza (the flu) virus, herpes simplex 1, streptococci, staphylococci, yeasts, and the bacteria that cause gum disease, cavities, and even diarrheal illness can survive --some even thrive-- in high numbers on toothbrushes. Because most people keep their toothbrushes in the bathroom, bacteria from the mouth are the major contaminant. Bacteria, molds, and fungi love moist environments, like the bathroom. They also do well in the dark, so the medicine cabinet provides an ideal environment for germs to survive and even multiply.Toothbrushes become infected some time between one week and one month after initial use. Many dental associations and most dentists recommend the proper housing for the used toothbrushes.
"Even after being rinsed visibly clean, toothbrushes can remain contaminated with potentially pathogenic organisms." --The Center for Disease Control
But even the many advisements in the field of oral hygiene products the protection and prevention of toothbrushes from microorganism has very least concern. Generally people immediate after the brushing onto the gums and teeth they wash tooth brush with tab water and keep the toothbrushes in open container upto next use i.e. at least minimum 8 hours. Flushing a toilet propels germs from the toilet into the air, where they can land on toothbrushes. Toothbrushes are usually stored together, so contact between family members' toothbrushes can spread germs from one brush to another. Prevention and protection of toothbrushes from common microorganisms as potential source of infection through oral route is now become a highly valuable in order to keep the toothbrushes hygienic for safe cleaning of teeth on the humans.

Description of our Invention:
[0002] The invention relates generally to a method for at-home used tooth brush cleansing using a light source instrument and a liquid for submerging of toothbrushes cleaning rinse containing an herbal agent as protection agent against the microorganism with derogatory effects as fluids and to eliminate harmful microorganism from bristle and surrounding areas of toothbrushes.
[0003] The prior art uses UV) radiation as method for sterilizing the used products. Normally ultra violet (UV) radiation is used in inoculation chamber or laminar air tlow. Expose the working area under UV radiation before half-an-hour or so prior to start the work. The source of UV radiation is generally UV -lamp or UV -tubes enclosed in quartz because glass will not transmit UV radiation. The radiation emitted by UV source cause damage to the cells by hydration & thymine dimer formation & produce lethal effect. Numerous studies have shown ultraviolet (UV) light to be effective in killing germs on toothbrushes, including bacteria, yeasts and viruses. A study suggest that UV Light Toothbrush Sanitizer eliminated up the 99.9% of germs tested on toothbrushes but they are fail to prevent the bacteria after treatment . We found many shortcomings in available methods of sterilization. Our invention provides the joint action for the complete removal bacterias and further prevention from the bacteria upto next use of tooth brush. The submersion of toothbrushes in novel herbal disinfectant liquid also gives the therapeutic effect onto the teeth.
[0004] Previous methods does not provide any device which can protect the tooth brush from developing the harmful microorganisms further no method or design is available for the housing and continues disinfecting of used tooth brushes upto next use. Open housing, tab water washing, and transfer of biological fluids, even after the normal washing of toothbrush from oral cavity is the some most common cause of microorganism development and till the date there is no method available for the housing

of used toothbrushes expect to keep them in open. In the prior art. there are no proven methods which provides the housing for used toothbrushes which protect and prevent the microbial growth in used toothbrushes and retain the pathogens from entering into the oral cavity during next brushing and further prevents the people from various diseases caused due to the pathogens and further provide effective protection from many diseases and herbal liquid made the fine films onto the bristles and give antibacterial effect in the oral cavity.
[0005] In addition, the contents of invented kit that is for sterilizing toothbrushes, designed primarily for use in a home, office or other medical setting, by a common men for oral hygiene.
[0006] The present invention provides a method for self-dental care with a light-providing dental device and a herbal liquid that contains a antimicrobial agent which is safe for at- home for oral use.
OBJECTIVES AND BRIEF SUMMARY OF THE INVENTION
[0007] It is an aim of the present invention to address the need for a used toothbrushes cleaning method that has a significant preventive and destructive effect on bacteria, viruses, and other microbes, while being generally safe for self-sensitization. An toothbrushes cleaning method is designed for prevention of bacteria, viruses, and other microbes, developed due to the deposited material from the oral cavity, including teeth, plaque, tongue, inner cheek tissue, gums, subginivival plaque, food or other residues, palate, and salivary glands.

[0008] It is a further aim of this invention is to record the joint effects of novel kit apparatus and Herbal disinfectant liquid on the used toothbrush for the prevention and protection of toothbrushes from diseases causing microorganisms. The microbial analysis of both a used toothbrush, wash normally and treated toothbrush in our apparatus and Herbal disinfectant liquid.
[0009| The present invention relates to "A novel kit which enables the toothbrushes users to prevent and protect the toothbrushes common disease causing microorganisms. The kit will provide the housing to toothbrushes immediate after the use upto next use".
[0010] The present invention provide the design and method for the kit which enables toothbrushes users a housing of toothbrushes, the design of toothbrushes housing will provide the keeping area for brush. Sensitization mechanism of used toothbrushes includes prevention and destroying of microorganisms in used toothbrushes upto next use. Weproposed that submersion of toothbrushes in herbal liquid and housing inio the kit apparalus reduces the 99% o\' cuntannnaling organisms. The submersion of botbbrshes in herbal liquid and bousing into the kit apparatus also prevent thegrowth of 1 albicans.

[0011] Procedure for microbial analysis
Microbial determination is done by taking sample of the extract in Soybean casein digest medium and incubated in test tube in a BOD Incubator. (NSW-151, Universal) at 35°C 37°C for about 24 hrs. The enriched sample is poured in following specific media for different bacterial pathogens.
1) TAMC (Total Aerobic Microbial Count)-SCDA (Soybean Casein Digest Agar (Pour plating)
2) TYMC (Total Yeast Mold Count)-SDA (Sabouraud Dextrose Agar)
3) E. coli-MacConkey Agar
4) S. aureus- Mannitol salt agar
5) P. aeruginosa- Cetrimide agar
6) Salmonela- Brilliant Green Agar
We have analyzed the used more then 100 used by their used index i.e 2day 5day, 10days and 15days and 30 days intervals for microbial load by common microbiological analysis methods. Results of our study revels that all the used toothbrushes is kept in open after use carrying the common dieses causing pathogens. Whereas non used and packed toothbrushes exhibits absent pathogens.
[0012] The focus of the invention is to highlight the value of prevention and protection of toothbrushes from microorganisms after their using. In the pre.sent boom of oral hygiene products the alarming and emerging problem for the users of toothbrushes is to protect and preventing their toothbrushes from microorganisms, which are the cause of most common of various dieses in humans.

[0013] The present invention involves the use of a light as source which provides the dental device which prevents and destroy the harmful microorganisms onto the toothbrushes. Further it prevents, deters diseases, inflammations, and infections caused due to microorganism.
[0014] Need of our invention:-Teeth are the common & essential part of our life. The market of teeth cleaning and oral hygiene is in billons. Three are various products in the marker which claims to protect the teeth and oral health by destroying the bacteria's which are responsible for tooth problems.
Examples
Fixample 1:
Method for the collection of toothbrushes- We have taken 100 used toothbrushes and subject them for microbiological testing then we kept all the tooth brushes into our novel kit apparatus and submerged it into the herbal disinfectant liquid and again examined them for the detection of diseases causing pathogens.
Protocol for Microbial study
We have taken the Swab from the bristles of the toothbrushes without the treatment and after the treatment in novel kit apparatus .We deep the swab into 1ml peptone water in test tube .Then we mix the swab properly in the peptone.We have further done the dilutions of peptone water by taking one milliliter of the water from the toothbrush and adding it to 9 milliliters of water.

We prepared the 10-1 dilution.
Toothbrush-water 1/10 100
10-1 1/10 1.000
10-2 1/10 10.000
10-3 1/10 100,000
10-4 1/10 1,000,000
10-5 1/10 10,000,000
You plate 1/10 of a milliliter.
Then plate 1/10 of a milliliter and incubated with 9 ml Soybean casein digest medium and incubated in test tube in a BOD Incubator.(NSW-151.Universal) at 35°C - 37°C for 24 hrs. The enriched sample is poured in following specific media for different bacterial pathogens.
1) TAMC-SCDA (Pour plating) 2)TYMC-SDA
3) E. coli-MacConkey Agar
4) S. aureus- Mannitol salt agar
5) P. aeruginosa- Cetrimide agar
6) Salmonela- Brilliant Green Agar

TEST ORGANISMS: The test organisms sources are ATCC.
TABLE 1 . RESULT OF MICROBIOLOGICAL TEST

(Table Removed)
Example 2:
Determination bacterial activity of herbal disinfectant liquid.
A well of 6 mm. diameter is prepared in nutrient agar with cork barer in steroid condition and sealed with 2% agar. 100 ml of log face broth culture of respective bacterial strains is spread on the plate O.SOmg extract is poured with absolute alcohol (AR grade), which serves as control. The plate is incubated at 37°C for 24-48 hours. The microbial growth inhibition is determined as the diameter of zone of inhibition around the well in mm. All turbid TSBs were subcultured to verify the presence of C. albicans.

Example 3:
We have conducted the in-vitro experiments on various oral hygiene products and found that what is claming is not accurate. In our study we have collected the used toothbrushes from volunteers for this trial.
Example 4:
We have collected the 100 toothbrushes and randomly distributed the toothbrushes further we evaluate all the toothbrushes for microbial load our results reveled that all toothbrushes after brushing on teethes kept and exposed in open found heavy microbial load and all the toothbrushes carrying many disease causing bacteria.
Example 5:
In common practices generally after cleaning the teeth the toothbrushes kept in a toothbrushes stands in open in which small oral cells serve as nutrition to bacteria and further uses for brushing in next day which provides the easy route to bacteria through oral cavity and we hypothesized that it is the common cause of many dangers disease in humans, causes and is full of products related with healthy teeth but no attention has been given by any person or community about the side effects & cross contamination between tooth, gums, mouth, and thorax by exposed toothbrushes.
Example 6:
We have undertaken this study to demonstrate that how toothbrushes can transmit disease to the human. Edmond Locard gave a law which is related with crime research & famous as a Principle of Exchange. This law is playing a very important role in Forensic researches. According to Edmond Locard when two things come into contact they mutually transfer the materials. Our study is based on the scientific principle.

[0020] A method and material for disinfecting of toothbrush for eliminating the cross contamination by the toothbrushes in oral cavity.
[0021] The cleaning and disinfecting activity was done by using a source of light in the ultra violet range in air tight box with herbal disinfected liquid which will provide the completely safe oral hygiene composition possessing a disinfecting activity on broad spectrum of microorganisms.
[0022] In another embodiment of invention is a kit which prevents and eliminates the harmful microorganisms by use of ultra violet light source in air tight box comprising with filled herbal disinfected liquid.
[0023] in another embodiment of invention is the air tight box use as housing of used toothbrushes after brushing onto the tooth's, gums and tongue. Further the keeping of objects such as toothbrushes, shaving blades and other regular toiletry items will provide the housing to these items and remain them as sterile upto next use.
[0024] In another embodiment of invention involves the use of ultra violet light tight box with herbal disinfected liquid which protect the object form developing of the microorganism, as the toothbrushes is most vulnerable site for the developmeni of microorganism and further destroys the harmful microorganisms which may be present onto the bristles. It will prevent oral diseases, intlammations. and infections caused bv the harmful microorganism.
[0025] The principle of our invention is supported by thePrinciple of Exchange given by Edmond Locard, we presume that the when the bristles of toothbrush was enter into the mouth and come into the contact with gums they exchange some tissues and other biological material from the oral cavity, which adhere with the bristles and it

surroundings. This biological material onto bristles and other surrounding area of toothbrushes serve as good media to develop the microorganism.
[0026] Unfortunately due to unawareness of oral hygiene the toothbrush are only washed with the common tab water which is the very common source of E-coli and other disease causing pathogens. The hypothesis of the experiment is that when the toothbrush insert into the mouth & rub onto the surface of the gums it contaminate with the transfer o\' some tissue and biological material due to pressure and rubbing of oral cavity.
We presume that the following route is the cause of the development of microorganism.
1. Inserting of toothbrushes into oral cavity.
2. Rubbing of toothbrushes into the oral cavity.
3. Washing of toothbrush tab water which is the very common source of h-coli other disea,se causing pathogens.
4. Remaining of the some biological tluids even after the washing of toothbrushes.
5. Keeping of toothbrushes in open container, which allows the microorganisms to develops within hours due to the availably of air, moisture and biological fluids as good growing medium.
6. Inserting of contaminated toothbrushes into the oral cavity and transfer of harmful microorganism into the human system and causes the many bacteria causing diseases.

What is claimed is:
1. Pavlight is the first toothbrush sanitization system with small and sleek design size of an average pencil box for the housing of used toothbrushes for the removal and prevention of microbial development upto next use of toothbrush.
2. Housing claimed in claim 1 comprising the cleaning and disinfecting activity by using a source of light in the ultra violet range.
3. Ultra violet light source claimed in claim 2 comprising the air tight box with
herbal disinfected liquid
4. Herbal disinfected liquid claimed in claim 3 provides the completely safe oral hygiene composition possessing a disinfecting activity on broad spectrum of microorganisms.
5. Disinfecting activity claimed in claim 4 is the another embodiment of invention is a complete kit which prevents and eliminates the harmful microorganisms by use of ultra violet light source in air tight box comprising with filled herbal disinfected liquid.
6. Elimination and prevention of the harmful microorganisms from used toothbrushes claimed in claim 5 of the invention is the air tight box use as housing of used toothbrushes after brushing onto the tooth's, gums and tongue. Further the keeping of objects such as toothbrushes, shaving blades and other regular toiletry items would provides the housing to these items and remain them as sterile upto next use.

7. Housing of used toothbrushes after brushing onto the tooth's, gums and tongue claimed in claim 6 involves the use of ultra violet light tight box with herbal disinfected liquid which protect the object form developing of the microorganism, as the toothbrushes is most vulnerable site for the development of microorganism and further destroys the harmful microorganisms which may be present onto the bristles. It will prevent oral diseases, inflammations, and infections caused by the harmful microorganism.
8. The prevention and disinfecting activity of harmful microorganisms present onto the bristles and surrounding areas of toothbrushes claimed in claim 7 provides the safe inserting of toothbrushes into the oral cavity for brushing and rubbing and prevent the transfer of harmful microorganism into the human system and causes the many bacteria causing diseases.