FIELD OF INVENTION
[001] The present disclosure relates to a composition for inhibiting growth of microorganisms on skin, said composition comprising synergistic concentrations of benzethonium chloride and at least one essential oil. Also provided are formulations comprising said compositions, and methods of preparing the same.
BACKGROUND OF THE INVENTION
[002] Microorganisms that generally reside in follicles, pores and surface of human skin are referred to as microflora. A majority of such microorganisms are gram-positive and are beneficial in occupying a niche and denying access to transients, which may be harmful, infectious and pathogenic. Some examples of such microflora are Staphylococcus epidermidis, Staphylococcus aureus (S. aureus), and Gram-Negative Bacilli (Escherichia coli). Different microflora have different properties and cause different diseases. For instance, Staphylococcus aureus is a common resident of skin and mucous membranes of humans. The Staphylococcus aureus is a common cause of skin infections such as boils, heat rash, also referred to as prickly heat. Further, Staphylococcus aureus cause some life threatening bacterial infections. Other diseases caused by such microflora include Toxic shock syndrome, Scalded skin syndrome, food poisoning.
[003] Antimicrobial products such as, emulsions, lotions, creams, ointments, salves, powders, suspensions, gels, soaps, are available to control multiplication of the microflora and pathogenic microorganisms present on the skin. Prior art describes antimicrobial products that contain antimicrobial agents used to kill pathogenic microorganisms. However high concentrations of such antimicrobial agent is required to kill a specific pathogenic microorganism, and this has an undesirable side effect of killing friendly and useful microorganisms.

SUMMARY OF THE INVENTION
[004] In an aspect of the present disclosure, there is provided a composition comprising synergistic concentrations of (a) benzethonium chloride, and (b) at least one essential oil selected from the group consisting of Ajwain seed oil (Trachyspermum copticum), Lavender tea tree oil (Melaleuca ericifolia) and linalool (Ex Basil).
[005] In an aspect of the present disclosure, there is provided a formulation comprising of a composition, said composition comprising synergistic concentrations of (a) benzethonium chloride, and (b) at least one essential oil selected from the group consisting of Trachyspermum copticum oil, Melaleuca ericifolia oil and linalool (Ex Basil).
[006] In an aspect of the present disclosure, there is provided a personal cleansing formulation comprising (a) at least one fatty acid in a range of 10 to 25% by weight; (b) at least one alkali in a range of 0.001 to 5% by weight; (c) at least one solubilizer in a range of 5 to 40% by weight; (d) optionally at least one chelating agent in a range of 0.01 to 5 % by weight; (e) benzethonium chloride in a range of 0.018 - 0.075 ppm; (f) Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm ox Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and (g) water in a range of 30 to 70 % weight.
[007] In an aspect of the present disclosure, there is provided a personal cleansing formulation comprising (a) at least one surfactant in a range of 2 to 20% by weight; (b) at least one polymer in a range of 0.1 to 15% by weight; (c) at least one solubilizer in a range of 0 to 10% by weight; (d) benzethonium chloride in a range of 0.018 - 0.075 ppm; (e) Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and (f) sufficient quantity of water to make up 100 % weight.
[008] In an aspect of the present disclosure, there is provided a personal cleansing formulation comprising (a) at least one fatty acid in a range of 10 to 25% by weight;

(b) at least one alkali in a range of 0.001 to 5% by weight; (c) at least one solubilizer in a range of 5 to 40% by weight; (d) optionally at least one chelating agent in a range of 0.001 to 5 % by weight; (e) benzethonium chloride in a range of 0.018 -0.037 ppm; (f) linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and (g) water in a range of 30 to 70 % weight.
[009] In an aspect of the present disclosure, there is provided a personal cleansing formulation comprising (a) at least one surfactant in a range of 2 to 20% by weight; (b) at least one polymer in a range of 0.1 to 15% by weight; (c) at least one solubilizer in a range of 0 to 10% by weight; (d) benzethonium chloride in a range of 0.018 - 0.037 ppm; (e) linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and (f) sufficient quantity of water to make up 100 % weight.
[0010] In an aspect of the present disclosure, there is provided a soap bar formulation comprising (a) at least one sodium salts of fatty acids in a range of 30-95%) by weight; (b) at least one humectant and emollient in a range of 0.5 to 10 % by weight; (c) at least one binder in a range of 0.5 to 10% by weight; (d) optionally at least one opacifier in a range of 0.001 to 3% by weight; (e) benzethonium chloride in a range of 0.018 - 0.075 ppm; (f) Trachyspermum copticum oil in a range of 195.31 -781.25 ppm or Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and (g) sufficient quantity of water to make up 100%) weight.
[0011] In an aspect of the present disclosure, there is provided a soap bar formulation comprising (a) at least one sodium salts of fatty acids in a range of 30-95%) by weight; (b) at least one humectant and emollient in a range of 0.5 to 10 % by weight; (c) at least one binder in a range of 0.5 to 10% by weight; (d) optionally at least one opacifier in a range of 0.001 to 3% by weight; (e) benzethonium chloride in a range of 0.018 - 0.037 ppm; (e) linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and (g) sufficient quantity of water to make up 100%) weight.
[0012] In an aspect of the present disclosure, there is provided a topical cosmetic formulation comprising:

i. at least one chelating agent in a range of 0.001-5% by weight;
ii. at least one polymer in a range of 0.005-5% by weight;
iii. at least one emulsifying agent in a range of 0.01-5%) by weight;
iv. at least one humectant in a range of 0.01 -10% by weight;
v. at least one preservative in a range of 0.001-1%) by weight;
vi. at least one neutralizing agent in a range of 0-0.75% by weight;
vii. benzethonium chloride in a range of 0.018 - 0.075 ppm; viii. Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and
ix. water to make up 100%> weight. [0013] In an aspect of the present disclosure, there is provided a topical cosmetic formulation comprising:
i. at least one chelating agent in a range of 0.001-5%) by weight;
ii. at least one polymer in a range of 0.005-5%) by weight;
iii. at least one emulsifying agent in a range of 0.01-5%) by weight;
iv. at least one humectant in a range of 0.01 -10% by weight;
v. at least one preservative in a range of 0.001-1%> by weight;
vi. at least one neutralizing agent in a range of 0-0.75% by weight;
vii. benzethonium chloride in a range of 0.018 - 0.037 ppm; viii. linalool (Ex Basil) in a range of 1562.5 - 2500 ppm;
ix. water to make up 100% weight. [0014] These and other features, aspects, and advantages of the present subject matter will be better understood with reference to the following description and appended claims. This summary is provided to introduce a selection of concepts in a simplified form. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter.

BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS
[0015] The detailed description is described with reference to the accompanying figures. In the figures, the left-most digit(s) of a reference number identifies the figure in which the reference number first appears. The same numbers are used throughout the drawings to reference like features and components. [0016] Figure 1 is a schematic of the checkerboard microbial assay to identify synergistic combination of actives that inhibit growth of microorganisms on human skin, in accordance with an embodiment of the present disclosure.
DETAILED DESCRIPTION OF THE INVENTION
[0017] Those skilled in the art will be aware that the present disclosure is subject to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The disclosure also includes all such steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations of any or more of such steps or features.
Definitions
[0018] For convenience, before further description of the present disclosure, certain
terms employed in the specification, and examples are collected here. These
definitions should be read in the light of the remainder of the disclosure and
understood as by a person of skill in the art. The terms used herein have the meanings
recognized and known to those of skill in the art, however, for convenience and
completeness, particular terms and their meanings are set forth below.
[0019] The articles "a", "an" and "the" are used to refer to one or to more than one
(i.e., to at least one) of the grammatical object of the article.
[0020] The terms "comprise" and "comprising" are used in the inclusive, open
sense, meaning that additional elements may be included. It is not intended to be
construed as "consists of only".

[0021] Throughout this specification, unless the context requires otherwise the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated element or step or group of element or steps but not the exclusion of any other element or step or group of element or steps. [0022] The term "including" is used to mean "including but not limited to". "Including" and "including but not limited to" are used interchangeably. [0023] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference.
[0024] A composition comprising "synergistic activity" or a "synergistic composition" is a combination of compounds which exhibits increased biological or functional activity as a non-linear multiple of the biological or functional activity of the individual compounds. In other words, the combined biological or functional activity of two or more compounds being tested is significantly greater than the expected result based on independent effects of the compounds when tested separately. Synergy may be apparent only at some ranges or concentrations. [0025] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods are clearly within the scope of the disclosure, as described herein. [0026] In an embodiment of the present disclosure, there is provided a composition comprising synergistic concentrations of (a) benzethonium chloride and (b) at least one essential oil selected from the group consisting of Trachyspermum copticum oil, Melaleuca ericifolia oil and linalool (Ex Basil).

[0027] In an embodiment of the present disclosure, there is provided a composition comprising synergistic concentrations of (a) benzethonium chloride in a range of 0.018 - 0.075 ppm and (b) at least one essential oil selected from the group consisting of Trachyspermum copticum oil, Melaleuca ericifolia oil and linalool (Ex Basil). [0028] In an embodiment of the present disclosure, there is provided a composition comprising synergistic concentrations of benzethonium chloride in a range of 0.018 -0.075 ppm and Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm. [0029] In an embodiment of the present disclosure, there is provided a composition comprising synergistic concentrations of benzethonium chloride in a range of 0.018 -0.075 ppm and Melaleuca ericifolia oil in a range of 3125 - 6250 ppm. [0030] In an embodiment of the present disclosure, there is provided a composition comprising synergistic concentrations of benzethonium chloride in a range of 0.018 -0.037 ppm and linalool (Ex Basil) in a range of 1562.5 - 2500 ppm. [0031] In an embodiment of the present disclosure, there is provided a composition comprising of (a) benzethonium chloride; and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein the said composition comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil in the range of 195.31 -781.25 ppm, and Melaleuca ericifolia oil in the range of 3125 - 6250 ppm. [0032] In an embodiment of the present disclosure, there is provided a composition comprising of (a) benzethonium chloride, and (b) linalool (Ex Basil), wherein the said composition comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) in the range of 1562.5 - 2500 ppm.
[0033] In an embodiment of the present disclosure, there is provided a composition comprising of a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil, or linalool (Ex Basil), wherein synergistic concentrations of benzethonium chloride in said composition is 0.018 ppm, Trachyspermum copticum

oil in said composition is 195.31 ppm or Melaleuca ericifolia oil in said composition is 3125 ppm or linalool (Ex Basil) in said composition is 1562.5 ppm. [0034] In an embodiment of the present disclosure, there is provided a composition comprising synergistic concentrations of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein the said composition inhibits growth of at least one microorganism which include but is not limited to Staphylococcus aureus (S. aureus) and Escherichia coli (E coli).
[0035] In an embodiment of the present disclosure, there is provided a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein synergistic concentration of (a) benzethonium chloride in the said composition is 0.018 ppm, and (b) Trachyspermum copticum oil in the said composition is 195.31 ppm, or Melaleuca ericifolia oil in the said composition is 3125 ppm or linalool (Ex Basil) in the said composition is 1562.5 ppm, wherein the said composition inhibits growth of at least one microorganism which include but is not limited to Staphylococcus aureus (S. aureus) and Escherichia coli (E coli).
[0036] In an embodiment of the present disclosure, there is provided a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil, wherein synergistic concentrations of (a) benzethonium chloride in the said composition is in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil in the said composition is in the range of 195.31 -781.25 ppm, or Melaleuca ericifolia oil in the said composition is in the range of 3125 - 6250 ppm, wherein the said composition inhibits growth of at least one microorganism which include but is not limited to Staphylococcus aureus (S. aureus) and Escherichia coli (E coli).
[0037] In an embodiment of the present disclosure, there is provided a composition comprising of (a) benzethonium chloride, and (b) linalool (Ex Basil), wherein

synergistic concentrations of (a) benzethonium chloride in the said composition is in
the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) in the said composition is
in the range of 1562.5 - 2500 ppm, wherein the said composition inhibits growth of at
least one microorganism which include but is not limited to Staphylococcus aureus
(S. aureus) and Escherichia coli (E coli).
[0038] In an embodiment of the present disclosure, there is provided a composition
comprising of a synergistic ratio of benzethonium chloride and Trachyspermum
copticum oil in the range of 1:2500 to 1:43500.
[0039] In an embodiment of the present disclosure, there is provided a composition
comprising a synergistic ratio of benzethonium chloride and Melaleuca ericifolia oil
in the range of 1: 41000 to 1:350000.
[0040] In an embodiment of the present disclosure, there is provided a composition
comprising a synergistic ratio of benzethonium chloride and linalool (Ex Basil) in the
range of 1:40000 to 140000.
[0041] In an embodiment of the present disclosure, there is provided a composition
comprising a synergistic ratio of benzethonium chloride and Trachyspermum
copticum oil in the range of 1:2500 to 1:43500, wherein the said composition inhibits
growth of at least one microorganism which include but is not limited to
Staphylococcus aureus (S. aureus) and Escherichia coli (E coli).
[0042] In an embodiment of the present disclosure, there is provided a composition
comprising a synergistic ratio of benzethonium chloride and Melaleuca ericifolia oil
in the range of 1: 41000 to 1:350000, wherein the said composition inhibits growth of
at least one microorganism which include but is not limited to Staphylococcus aureus
(S. aureus) and Escherichia coli (E coli).
[0043] In an embodiment of the present disclosure, there is provided a composition
comprising a synergistic ratio of benzethonium chloride and linalool (Ex Basil) oil in
the range of 1:40000 to 140000, wherein the said composition inhibits growth of at

least one microorganism which include but is not limited to Staphylococcus aureus (S. aureus) and Escherichia coli (E coli).
[0044] In an embodiment of the present disclosure, there is provided a formulation comprising of a) benzethonium chloride, and b) Trachyspermum copticum oil or Melaleuca ericifolia oil, wherein synergistic concentrations of (a) benzethonium chloride in the said composition is in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil is in the range of 195.31 - 781.25 ppm, or Melaleuca ericifolia oil is in the range of 3125 - 6250 ppm.
[0045] In an embodiment of the present disclosure, there is provided a formulation comprising of a) benzethonium chloride, and b) linalool (Ex Basil), wherein synergistic concentrations of (a) benzethonium chloride in the said composition is in the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) is in the range of 1562.5 -2500 ppm.
[0046] In an embodiment of the present disclosure, there is provided a formulation comprising of benzethonium chloride, and Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein synergistic ratios of (a) benzethonium chloride and Trachyspermum copticum oil is in the range of 1:2500 to 1:43500; or (b) synergistic ratio of benzethonium chloride and Melaleuca ericifolia oil is in the range of 1: 41000 to 1:350000; or (c) synergistic ratio of benzethonium chloride and linalool (Ex Basil) is in the range of 1:40000 to 140000. [0047] In an embodiment of the present disclosure, there is provided a formulation as described herein, wherein said formulation further comprising of one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, fragrances, surfactants, solubilizers, opacifiers, binders, preservatives, neutralizing agents, chelating agents, emulsifying agents, emollients and diluents.

[0048] In an embodiment of the present disclosure, there are provided a formulation comprising one or more cosmetically acceptable excipients, the excipients used for preparation of formulation is indicated in Table 1.
Table 1.

[0049] In an embodiment of the present disclosure, there is provided a formulation as described herein, wherein the said formulation further comprising of at least one i) polymer selected from the group consisting of (a) at least one starch; (b) at least one modified starch; (c) at least one gum; (d) at least one carbomer; (e) at least one silicone based polymer; (f) at least one acrylate; (g) at least one cellulose based polymer; and (h) combinations thereof; ii) chelating agent is selected from the group consisting of dimercapto-succinic acid (DMSA), dimercapto-propane sulfonate (DMPS), alpha lipoic acid (ALA), calcium disodium versante (CaNa2-EDTA), disodium EDTA, dimercaprol (BAL), etidronic acid, ethyleneglycoltetraacetic acid, ehtylenediaminedisuccinic acid, and combinations thereof; iii) emollient is selected from the group consisting of lanolin, octyldodecanol, hexyl decanol, oleyl alcohol, decyloleate, isopropyl stearate, isopropyl palmitate, isopropyl myristate, hexyl laureate, dioctyl cyclohexane, PPG-15 stearyl ether, isohexadecane, stearic acid, cetyl alcohol, mineral oil, at least one ester, at least one alcohol, at least one essential oil and extracts thereof, at least one silicone oil, linear polydimethylsiloxanes, cyclic polydimethylsiloxanes, volatile silicones, and combinations thereof; iv) humectant selected from the group consisting of at least one silicone, at least one silicone oil, at least one silicone polymer, at least one cyclized derivative of silicone, at least one polyol, at least one acrylate polymer, at least one biopolymer, and combinations thereof; v) emulsifying agent selected from the group consisting of oxyalkylenated fatty acid esters of polyols, PEG-100 stearate & glyceryl stearate, oxyalkylenated fatty acid esters of sorbitan, oxyalkylenated (oxyethylenated and/or oxypropylenated) fatty alcohol ethers, alkoxylated or non-alkoxylated sugar esters, sorbitan esters, esters of diacids, esters of fatty alcohols, acrylates, PEG based emulsifiers, polyglyceryl based emulsifiers, silicones, dimethicones, mixture of glycerylmonostearate and of polyethylene glycol stearate and combinations thereof; vi) fragrance selected from the group consisting of blend of essential oils, blend of synthetic aroma compounds, and combination thereof; and vii) cosmetically

acceptable additive is selected from the group consisting of colorants, antioxidants, free radical scavengers, vitamins, anti-aging agents, thickeners, and combinations thereof.
x. [0050] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of : (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil, wherein the composition comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil in the range of 195.31 - 781.25 ppm, ox Melaleuca ericifolia oil in the range of 3125 - 6250 ppm; and (ii) contacting the said composition with skin surface, wherein the said method inhibits growth of microorganisms.
[0051] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of : (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) linalool (Ex Basil), wherein the composition comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) in the range of 1562.5 - 2500 ppm; and (ii) contacting the said composition with skin surface, wherein the said method inhibits growth of microorganisms.
[0052] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of : (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein the composition comprises synergistic ratios of (a) benzethonium chloride and Trachyspermum copticum oil in

the range of 1:2500 to 1:43500, or (b) benzethonium chloride and Melaleuca ericifolia oil in the range of 1: 41000 to 1:350000 or (c) benzethonium chloride and linalool (Ex Basil) in the range of 1:40000 to 140000; and (ii) contacting said the composition with skin surface, wherein the said method inhibits growth of microorganisms.
[0053] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein the composition comprises a synergistic concentration of (a) 0.018 ppm of benzethonium chloride, and (b) 195.31 ppm of Trachyspermum copticum oil, or 3125 ppm of Melaleuca ericifolia oil or 1562.5 ppm of linalool (Ex Basil); and (ii) contacting the said composition with skin surface, wherein the said method inhibits growth of microorganisms. [0054] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of : (i) obtaining a composition comprising of (a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil, and (c) suitable carriers, diluents, cosmetically acceptable excipients, wherein the composition comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil in the range of 195.31 - 781.25 ppm, or Melaleuca ericifolia oil in the range of 3125 - 6250 ppm; and (ii) contacting the said composition with skin surface, wherein the said method inhibits growth of microorganisms.
[0055] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of : (i) obtaining a formulation comprising of (a) benzethonium chloride, (b) linalool (Ex Basil), and (c) one or more

cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) in the range of 1562.5 - 2500 ppm; and (ii) contacting the said formulation with skin surface, wherein the said method inhibits growth of microorganisms.
[0056] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of (i) obtaining a formulation comprising of (a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil, or linalool (Ex Basil) and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises a synergistic concentration of (a) 0.018 ppm of benzethonium chloride, and (b) 195.31 ppm of Trachyspermum copticum oil, or 3125 ppm of Melaleuca ericifolia oil or 1562.5 ppm of linalool (Ex Basil); and (ii) contacting the said formulation with skin surface, wherein the said method inhibits growth of microorganisms.
[0057] In an embodiment of the present disclosure, there is provided a method of preventing skin infections such as boils, heat rash, prickly heat by inhibiting growth of microorganisms, said method comprising the steps of (i) obtaining a formulation comprising of (a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation

comprises synergistic ratios of (a) benzethonium chloride and Trachyspermum copticum oil in the range of 1:2500 to 1:43500; or (b) benzethonium chloride and Melaleuca ericifolia oil in the range of 1: 41000 to 1:350000 or (c) benzethonium chloride and linalool (Ex Basil) in the range of 1:40000 to 140000; and (ii) contacting the said formulation with skin surface, wherein the said method inhibits growth of microorganisms.
[0058] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of : (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil ox Melaleuca ericifolia oil, wherein the composition comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil in the range of 195.31 -781.25 ppm, ox Melaleuca ericifolia oil in the range of 3125 - 6250 ppm; and (ii) contacting the said composition with the hard surface, wherein the said method inhibits growth of microorganisms.
[0059] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of : (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) linalool (Ex Basil), wherein the composition comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) in the range of 1562.5 - 2500 ppm; and (ii) contacting the said composition with the hard surface, wherein the said method inhibits growth of microorganisms. [0060] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of : (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein the composition comprises synergistic ratios of (a) benzethonium chloride and Trachyspermum copticum oil in the range of 1:2500 to 1:43500, or (b)

benzethonium chloride and Melaleuca ericifolia oil in the range of 1: 41000 to 1:350000 or (c) benzethonium chloride and linalool (Ex Basil) in the range of 1:40000 to 140000; and (ii) contacting said the composition with the hard surface, wherein the said method inhibits growth of microorganisms.
[0061] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of (i) obtaining a composition comprising of (a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein the composition comprises a synergistic concentration of (a) 0.018 ppm of benzethonium chloride, and (b) 195.31 ppm of Trachyspermum copticum oil, or 3125 ppm of Melaleuca ericifolia oil or 1562.5 ppm of linalool (Ex Basil); and (ii) contacting the said composition with the hard surface, wherein the said method inhibits growth of microorganisms.
[0062] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of : (i) obtaining a formulation comprising of (a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil, and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil in the range of 195.31 - 781.25 ppm, or Melaleuca ericifolia oil in the range of 3125 - 6250 ppm; and (ii) contacting the said formulation with the hard surface, wherein the said method inhibits growth of microorganisms.
[0063] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of : (i) obtaining a formulation comprising of (a) benzethonium chloride, (b)

linalool (Ex Basil), and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) in the range of 1562.5 - 2500 ppm; and (ii) contacting the said formulation with the hard surface, wherein the said method inhibits growth of microorganisms. [0064] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of (i) obtaining a formulation comprising of (a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil, or linalool (Ex Basil) and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises a synergistic concentration of (a) 0.018 ppm of benzethonium chloride, and (b) 195.31 ppm of Trachyspermum copticum oil, or 3125 ppm of Melaleuca ericifolia oil or 1562.5 ppm of linalool (Ex Basil); and (ii) contacting the said formulation with the hard surface, wherein the said method inhibits growth of microorganisms.
[0065] In an embodiment of the present disclosure, there is provided a method for disinfecting hard surfaces containing microorganisms, said method comprising the steps of (i) obtaining a formulation comprising of (a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises synergistic ratios of (a) benzethonium chloride and Trachyspermum copticum oil in the range of 1:2500 to 1:43500; or (b) benzethonium

chloride and Melaleuca ericifolia oil in the range of 1: 41000 to 1:350000 or (c) benzethonium chloride and linalool (Ex Basil) in the range of 1:40000 to 140000; and (ii) contacting the said formulation with the hard surface, wherein the said method inhibits growth of microorganisms.
[0066] In an embodiment of the present disclosure, there is provided a composition comprising of a) benzethonium chloride; and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein synergistic concentrations of
(a) benzethonium chloride in the said composition is 0.018 ppm, (b) Trachyspermum
copticum oil in the said composition is 195.31 ppm or Melaleuca ericifolia oil in the
said composition is 3125 ppm, or linalool (Ex Basil) in the said composition is
1562.5 ppm, and used for preparing topical formulations for inhibiting growth of
microorganisms.
[0067] In an embodiment of the present disclosure, there is provided a composition comprising of a) benzethonium chloride; and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), wherein synergistic ratios of (a) benzethonium chloride and Trachyspermum copticum oil is in the range of 1:2500 to 1:43500, or (b) benzethonium chloride and Melaleuca ericifolia oil is in the range of 1: 41000 to 1:350000, (c) benzethonium chloride and linalool (Ex Basil) is in the range of 1:40000 to 140000, and used for preparing topical formulations for inhibiting growth of microorganisms.
[0068] In an embodiment of the present disclosure, there is provided a formulation comprising of a) benzethonium chloride; and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil), and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein synergistic concentrations of (a) benzethonium chloride in the said formulation is 0.018 ppm, and
(b) Trachyspermum copticum oil in the said formulation is 195.31 ppm or Melaleuca

ericifolia oil in the said formulation is 3125 ppm or linalool (Ex Basil) in the said formulation is 1562.5 ppm, and used for preparing topical formulations for inhibiting growth of microorganisms.
[0069] In an embodiment of the present disclosure, there is provided a formulation comprising of a) benzethonium chloride, and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil; and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.075 ppm, and (b) Trachyspermum copticum oil in the range of 195.31 - 781.25 ppm, or Melaleuca ericifolia oil in the range of 3125 - 6250 ppm, and used for preparing topical formulations for inhibiting growth of microorganisms.
[0070] In an embodiment of the present disclosure, there is provided a formulation comprising of a) benzethonium chloride, and (b) linalool (Ex Basil); and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation comprises synergistic concentrations of (a) benzethonium chloride in the range of 0.018 - 0.037 ppm, and (b) linalool (Ex Basil) in the range of 1562.5 - 2500 ppm and used for preparing topical formulations for inhibiting growth of microorganisms.
[0071] In an embodiment of the present disclosure, there is provided a formulation comprising of a) benzethonium chloride, (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil); and (c) one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, solubilizer, fragrances, chelating agents, emulsifying agents, emollients and diluents, wherein the formulation

comprises synergistic ratios of (a) benzethonium chloride and Trachyspermum copticum oil in the range of 1:2500 to 1:43500, or (b) benzethonium chloride and Melaleuca ericifolia oil in the range of 1: 41000 to 1:350000, (c) benzethonium chloride and linalool (Ex Basil) in the range of 1:40000 to 140000,and used for preparing topical formulations for inhibiting growth of microorganisms. [0072] In an embodiment of the present disclosure, there is provided an anti¬microbial personal cleansing formulation comprising (a) at least one fatty acid in a range of 10 to 25% by weight; (b) at least one alkali in a range of 0.001 to 5% by weight; (c) at least one solubilizer in a range of 5 to 40% by weight; (d) optionally at least one chelating agent in a range of 0.001 to 5 % by weight; (e) benzethonium chloride in a range of 0.018 - 0.075 ppm; (f) Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm ox Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and (g) water in a range of 30 to 70 % by weight.
[0073] In an embodiment of the present disclosure, there is provided an anti¬microbial personal cleansing formulation comprising (a) at least one fatty acid in a range of 10 to 25% by weight; (b) at least one alkali in a range of 0.001 to 5% by weight; (c) at least one solubilizer in a range of 5 to 40% by weight; (d) optionally at least one chelating agent in a range of 0.001 to 5 % by weight; (e) benzethonium chloride in a range of 0.018 - 0.037 ppm; (f) linalool (Ex Basil) in a range of 1562.5 -2500 ppm; and (g) water in a range of 30 to 70 % by weight.
[0074] The personal cleansing formulation disclosed herein further comprises of at least one fatty acid selected from C8 to C18 fatty acids.
[0075] In another embodiment of the present disclosure, there is provided an anti-microbial personal cleansing formulation comprising (a) at least one surfactant in a range of 2 to 20% by weight; (b) at least one polymer in a range of 0.1 to 15% by weight; (c) at least one solubilizer in a range of 0 to 10% by weight; (d) benzethonium chloride in a range of 0.018 - 0.075 ppm; (e) Trachyspermum copticum

oil in a range of 195.31 - 781.25 ppm or Melaleuca ericifolia oil in a range of 3125 -6250 ppm; and (f) sufficient quantity of water to make up 100 % by weight. [0076] In another embodiment of the present disclosure, there is provided an anti¬microbial personal cleansing formulation comprising (a) at least one surfactant in a range of 2 to 20% by weight; (b) at least one polymer in a range of 0.1 to 15% by weight; (c) at least one solubilizer in a range of 0 to 10% by weight; (d) benzethonium chloride in a range of 0.018 - 0.037 ppm; (e) linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and (f) sufficient quantity of water to make up 100 % by weight.
[0077] The anti-microbial personal cleansing formulation disclosed herein further comprises of at least one surfactant selected from a group that includes amphoteric surfactants, anionic surfactants, non-ionic surfactants and combinations thereof. [0078] The anti-microbial personal cleansing formulation disclosed herein further comprises of at least one surfactant selected from the group that includes alkyl sulfates, alkyl ether sulfates, alkyl glucosides, anionic acyl sarcosinates, N-acyl glutamates, alkyl sulfosuccinates, alkyl betaines, alpha olefin sulfonates and combinations thereof.
[0079] In still another embodiment of the present disclosure, there is provided an anti-microbial soap bar formulation comprising (a) at least one sodium salts of fatty acids in a range of 30-95% by weight; (b) at least one humectant and emollient in a range of 0.5 to 10 % by weight; (c) at least one binder in a range of 0.5 to 10% by weight; (d) optionally at least one opacifier in a range of 0.001 to 3% by weight; (e) benzethonium chloride in a range of 0.018 - 0.075 ppm; (f) Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or Melaleuca ericifolia oil in a range of 3125 -6250 ppm; and (g) sufficient quantity of water to make up 100%) by weight. [0080] In still another embodiment of the present disclosure, there is provided an anti-microbial soap bar formulation comprising (a) at least one sodium salts of fatty acids in a range of 30-95%> by weight; (b) at least one humectant and emollient in a

range of 0.5 to 10 % by weight; (c) at least one binder in a range of 0.5 to 10% by
weight; (d) optionally at least one opacifier in a range of 0.001 to 3% by weight; (e)
benzethonium chloride in a range of 0.018 - 0.037 ppm; (e) linalool (Ex Basil) in a
range of 1562.5 - 2500 ppm; and (g) sufficient quantity of water to make up 100%
weight.
[0081] In still another embodiment of the present disclosure, there is provided a
topical cosmetic anti-microbial formulation comprising:
i. at least one chelating agent in a range of 0.001-5%) by weight; ii. at least one polymer in a range of 0.005-5%) by weight; iii. at least one emulsifying agent in a range of 0.01-5%) by weight; iv. at least one humectant in a range of 0.01 -10% by weight; v. at least one preservative in a range of 0.001-1%> by weight; vi. optionally, at least one neutralizing agent in a range of 0-0.75%) by
weight; vii. benzethonium chloride in a range of 0.018 - 0.075 ppm; viii. Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and ix. water to make up 100 % weight. [0082] In still another embodiment of the present disclosure, there is provided a topical cosmetic anti-microbial formulation comprising:
i. at least one chelating agent in a range of 0.001-5%) by weight; ii. at least one polymer in a range of 0.005-5%) by weight; iii. at least one emulsifying agent in a range of 0.01-5%) by weight; iv. at least one humectant in a range of 0.01 -10% by weight; v. at least one preservative in a range of 0.001-1%> by weight; vi. optionally, at least one neutralizing agent in a range of 0-0.75%) by
weight; vii. benzethonium chloride in a range of 0.018 - 0.037 ppm;

viii. linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and ix. water to make up 100 % weight. [0083] In an embodiment of the present disclosure, there is provided a method of preparing a topical anti-microbial formulation in the form of a hand wash comprising: (i) melting Laurie acid, Myristic acid and Palmitic acid at 75-80°C in water bath; (ii) preparing 10% neutralizing solution of KOH; (iii) neutralizing by adding KOH solution drop wise to benzethonium chloride with Trachyspermum copticum oil or Melaleuca ericifolia or linalool (Ex Basil) and mixing at 50-60 rpm; (iv) adding PEG-150 Distearate followed by adding Glycerin at 75°C and mixed at 50-60 rpm; (v) adding fragrance at 40°C and then adding Phenoxyethanol and mixing till all the components are mixed properly at 50-60 rpm, (vi) adding Sodium Chloride to adjust the viscosity, and (vii) adding water to make up 100 % weight. [0084] In an embodiment of the present disclosure, there is provided a method of preparing a formulation, wherein the formulation comprises a synergistic concentration of (a) 0.018 - 0.075 ppm of benzethonium chloride, and (b) 195.31 -781.25 ppm of Trachyspermum copticum oil, or 3125 - 6250 ppm of Melaleuca ericifolia oil.
[0085] In an embodiment of the present disclosure, there is provided a method of preparing a formulation, wherein the formulation comprises a synergistic concentration of (a) 0.018 - 0.037 ppm of benzethonium chloride, and (b) 1562.5 -2500 ppm of linalool (Ex Basil).
[0086] In an embodiment of the present disclosure, there is provided a method of preparing a formulation, wherein the formulation comprises a synergistic concentration of (a) 0.018 ppm of benzethonium chloride, and (b) 195.31 ppm of Trachyspermum copticum oil or 3125 ppm of Melaleuca ericifolia oil or 1562.5 ppm of linalool (Ex Basil).
[0087] In an embodiment of the present disclosure, there is provided a method of preparing a formulation, wherein the formulation comprises synergistic ratios of (a)

benzethonium chloride and Trachyspermum copticum oil is in the range of 1:2500 to
1:43500, or (b) benzethonium chloride and Melaleuca ericifolia oil is in the range of
1: 41000 to 1:350000 or (c) benzethonium chloride and linalool (Ex Basil) is in the
range of 1:40000 to 140000.
[0088] In an embodiment of the present disclosure, there is provided a composition
which can be formulated in the form of dispersion, emulsion, lotion, cream, ointment,
balm, salve, powder, suspension, gel, aerosol, foam, micelle, soap bar, wipe, liquid
crystal or encapsulation.
[0089] In an embodiment of the present disclosure, there is provided a formulation
selected from the group consisting of soap, shower gel, hand wash, face wash, body
wash, hand / body sanitizer dusting powder, shampoo, conditioner, deodorant, body
spray, wet wipes, anti-microbial/antiseptic/disinfectant liquid or gel, lip stick, skin
cream, lip balm, foundation, or makeup.
[0090] Although the subject matter has been described in considerable detail with
reference to certain preferred embodiments thereof, other embodiments are possible.
EXAMPLES
[0091] The disclosure will now be illustrated with working examples, which is
intended to illustrate the working of disclosure and not intended to be taken
restrictively to imply any limitations on the scope of the present disclosure.
[0092] Unless defined otherwise, all technical and scientific terms used herein have
the same meaning as commonly understood to one of ordinary skill in the art to
which this disclosure belongs.
[0093] Although methods and materials similar or equivalent to those described
herein can be used in the practice of the disclosed methods and compositions, the
exemplary methods, devices and materials are described herein. It is to be understood
that this disclosure is not limited to particular methods, and experimental conditions
described, as such methods and conditions may apply.

Materials and methods
[0094] Benzethonium Chloride: Benzethonium chloride, also known as hyamine is a synthetic quaternary ammonium salt. Benzethonium chloride exhibits a broad spectrum of microbiocidal activity against bacteria, fungi, mold and viruses. Independent testing shows that benzethonium chloride is highly effective against such pathogens as methicillin-resistant Staphylococcus aureus, Salmonella, Escherichia coli, Clostridium difficile, hepatitis B virus, hepatitis C virus, herpes simplex virus (HSV), human immunodeficiency virus (HIV), respiratory syncytial virus (RSV), and norovirus.
[0095] Trachyspermum copticum oil: Trachyspermum copticum oil is obtained by steam distillation and is a potent antimicrobial agent which shows antimicrobial as well as antioxidants properties. Studies showed that Trachyspermum copticum oil has inhibitory effect against E. coli, K. pneumonia and S. aureus. It is also active against even multi drug resistant microbial pathogens.
[0096] Melaleuca ericifolia oil: Commonly known as swamp paper bark, is a plant in the myrtle family, Myrtaceae and the genus Melaleuca, native to south-eastern Australia. Traditionally Oil from the leaves was used for medicine. Melaleuca ericifolia leaves have been analyzed for their oil content and found to contain mostly 1,8-cineole (Eucalyptol) but also significant amounts of a-pinene. The aromatic oil in the leaves has been used for dental and medical preparations and in scented soaps. [0097] Linalool (Ex Basil): linalool (Ex Basil) is a naturally occurring terpene alcohol found in many flowers and spice plants. For ex. Lavender oil and Bergamot oil etc. Oil from leaves ofBursera aloexylan was found to contain a high linalool (Ex Basil) level (96.7%) and antimicrobial test indicates that oil was effective against Staphylococcus epidermides (0.15mg/ml) and Rhodococcus e^w/'(0.60mg/ml). [0098] Benzethonium Chloride was obtained from Qualigens. [0099] Trachyspermum copticum oil was obtained from IL Health & Beauty Natural Oils Co. (US)

[00100] Melaleuca ericifolia was obtained from Down Under enterprises (Australia).
[00101] linalool (Ex Basil) was obtained from Zhejiang Novorate Biotech Co., Ltd.
[00102] Mueller Hinton II Broth (cation-adjusted) (MHB) was obtained from BD.
[00103] Nutrient Broth (NB) was obtained from Hi Media.
[00104] Resazurin was obtained from Sigma Aldrich.
[00105] Staphylococcus aureus (MTCC 96) was obtained from Microbial Type
Culture Collection and Gene Bank (MTCC).
Anti-microbial activity assay:
Preparation of sample solutions and dye
[00106] Benzethonium Chloride: A stock of lmg/ml (1000 ppm) was prepared in
Ethanol. Further, a working stock of 9.7 ppm was prepared in MHB Broth.
[00107] Trachyspermum copticum oil: A stock of approx. 400 mg/lml (4,00,000
ppm) was prepared in MHB Broth.
[00108] Melaleuca ericifolia Oil: A stock of approx. 800 mg/lml (8,00,000 ppm)
was prepared in MHB Broth.
[00109] Linalool (Ex Basil): A stock of approx. 400 mg/lml (4,00,000 ppm) was
prepared in MHB Broth.
[00110] Resazurin: The stock of lmg/ml was prepared in sterile saline. Further, a
working solution was prepared by dissolving 1ml of stock solution in 4 ml of sterile
saline to get 200ug/ml solution.
Inoculum Preparation
[00111] A loopful of S. aureus culture was cultured in nutrient broth and incubated at 37°C for 24 hours under aerobic condition. Post incubation, the inoculum size was
o
adjusted to 0.1 absorbance at 600 nm (corresponding to approximately 1><10 CFU/mL) using spectrophotometer. Further diluted 100 fold in MHB broth to a concentration of approximately 106 CFU/ml.

Minimum Inhibitory Concentration (MIC) and its determination
[00112] Minimum inhibitory concentration (MIC) is the lowest concentration of an active that inhibits visible growth of bacteria. The MIC of an active is determined by preparing solutions of the active at increasing concentrations, incubating the solutions with the separate batches of cultured bacteria, and measuring the results using agar dilution or broth microdilution.
[00113] MIC of sample solution is determined by following these steps: A volume of 100 ul sterile nutrient broth was added to all 96 wells except first three wells of the round bottom microtitre plate A1B1C1 to which 200 ul of above prepared sample solution was added. From the first column A1B1C1, 100 ul of the sample was transferred to the first three wells of second column (A2B2C2) of plate and hence the sample serially diluted till D12E12F12 and 100 ul of the sample discarded from D12E12F12 to maintain same volume of the solution in all wells. To the wells containing sample, 100 ul bacterial suspension (106 CFU/ml) was added such that the final inoculum is 105 CFU/mL and the sample concentration is halved. Further Gl to G12 that contains bacterial cell suspension + broth medium 100 ul each, served as growth control and HI to H12 that contains only broth medium 200 ul, served as the broth control. The plates were then incubated at 37° C for 24 hours. After 24 hours of incubation, 10 ul of working solution of resazurin (200 ug/ml) was added to all wells. The plates were wrapped with aluminium film and incubated at 37° C for 1 hour. Post incubation, the color change was then assessed visually. Any color change from purple to pink was recorded as positive and indicated growth of microorganisms. This experiment was performed in triplicate under aseptic conditions.
Example 1
[00114] Effect of benzethonium chloride, Trachyspermum copticum oil, Melaleuca ericifolia oil and linalool (Ex Basil) on growth of S. aureus. Table 2. indicates the individual minimum inhibitory concentrations (MIC) for benzethonium chloride,

Trachyspermum copticum oil, Melaleuca ericifolia oil and linalool (Ex Basil) for inhibiting the growth of S. aureus.
Table 2.
Individual MIC for benzethonium chloride, Trachyspermum copticum oil, Melaleuca ericifolia oil and linalool (Ex Basil)

Checkerboard Assay method:
[00115] The checkerboard assay method provides synergistic concentration testing of anti-microbial compositions, the method determines interaction and potency of two actives when used concurrently. Using Lorian methodology, this assay method determines the effect of the combination of two actives on potency in comparison to their individual activities, and then represented as the Fractional Inhibitory Concentration (FIC) index value. To quantify the interactions between the two actives being tested, the FIC index (the combinations of actives that produced the greatest change from the individual active MIC) value is calculated for each microorganism species and the combination of actives, the steps involved in calculation are as follows:
FIC of Sample A = MIC of sample A in combination with sample B
MIC of sample A alone

FIC of Sample B= MIC of sample B in combination with Sample A
MIC of sample B alone
FIC index = FIC of Sample A + FIC of Sample B
The FIC index is interpreted to infer the effect of various combinations of active:
A concentration of combination of actives is inferred as synergistic if FIC index ^0.5
A concentration of combination of actives is inferred as indifferent if 2 > FIC index > 0.5
A concentration of combination of actives is inferred as antagonistic if FIC index > 2
Method of determining synergistic concentration of benzethonium chloride and an essential oil using checkerboard Assay:
[00116] The checkerboard assay is carried out through following steps. The concentrations tested for each sample were set four or five dilutions below as well as above the expected MIC. A 96 round bottom well microtitre plate as shown in Fig 1. was taken, 50 ul of the MUB broth added to all the wells and 100 ul of the broth added to well in 12th column, which acts as a sterility control. 50 ul of benzethonium chloride (Sample A) was added in the first column (Al to HI) and serially diluted on the x-axis till the 10th column (A10 to H10) where 50ul of the solution was discarded to maintain same volume of the solution in all wells. 50 ul of Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil) (Sample B) was added in the row (Al to A10), in which each row contains the same amount of Trachyspermum copticum oil or Melaleuca ericifolia Oil or linalool (Ex Basil) (Sample B) being diluted on column (A to H). As a result, each well in the checkerboard contains a unique combination of the two samples being tested. 100 ul

bacterial suspension (106 CFU/ml) of S. aureus culture was added to all the wells. A sterility control was set up from Al2 to HI2, and growth control (100 jal media +100 ul bacterial cell suspension) was setup from All to Hll. The plates were incubated at 25°C for 24 hours under aerobic condition. Based on the growth/inhibition of the microbial cultures, the MIC at various concentrations was determined. The MICs determined were used for calculating the synergistic/indifferent/ antagonistic effects. For each checkerboard, an MIC plate (for samples alone) was setup in parallel. MIC of the combination was compared with the MIC of the actives alone and the fractional inhibitory concentration values (FIC index) were determined as described in the Checkerboard Assay Method.
Example 2
[00117] Effect of combination of benzethonium chloride and Trachyspermum copticum oil at various concentrations on the growth of microorganism S. aureus. [00118] Results of test for synergistic effect of benzethonium chloride and Trachyspermum copticum oil samples. Combination of benzethonium chloride and Trachyspermum copticum oil at various concentrations was tested for synergistic effect on inhibiting growth of S. aureus and is indicated in Table 3.
Table 3.
Results of synergistic effect of benzethonium chloride and Trachyspermum copticum
oil.


MIC of ti: T = MIC of benzetfiomum cfilonde in combination with Trachyspermum copticum oil.
MIC of T: B = MIC of in Trachyspermum copticum oil in combination with benzethonium chloride.
The results of the above test indicate that a combination of benzethonium chloride in the range of 0.018 ppm to 0.075 ppm, and Trachyspermum copticum oil in the range of 195.31 ppm to 781.25ppm, surprisingly shows synergy in inhibiting the growth of microorganism S. aureus.
[00119] Individual MIC data for benzethonium chloride and Trachyspermum copticum oil samples is indicated in Table 4.
Table 4. Individual MIC of benzethonium chloride and Trachyspermum copticum oil

The results of above test indicate individual MIC of 0.244 ppm for benzethonium chloride and 3125 ppm for Trachyspermum copticum oil.

[00120] The results of test for combined concentrations of benzethonium chloride and Trachyspermum copticum oil samples, that indicate indifferent effect on inhibition of S. aureus is indicated in Table 5.
Table 5.
Results of combination of benzethonium chloride and Trachyspermum copticum oil
with indifferent effect.
ii i i i i i i
MIC of B: A = MIC of benzethonium chloride in combination with Trachyspermum copticum oil
MIC of A: B = MIC of in Trachyspermum copticum oil in combination with benzethonium chloride
The results of above test indicate a combination of benzethonium chloride in the range of 0.15 ppm to 0.244 ppm, and Trachyspermum copticum oil in the range of 1562.5 to 3125 ppm that shows indifferent effect on the inhibition of growth of S. aureus.
Example 3
[00121] Effect of combination of benzethonium chloride and Melaleuca ericifolia oil on growth/inhibition of S. aureus. Combination of benzethonium chloride and Melaleuca ericifolia oil at various concentrations was tested for its effect on inhibiting growth of S. aureus.
[00122] The synergistic effect of benzethonium chloride and Melaleuca ericifolia oil samples is indicated in Table 6.

Table 6.
Results of test for synergistic effect of benzethonium chloride and Melaleuca
ericifolia oil

MIC of B: M= MIC of benzethonium chloride in combination with Melaleuca ericifolia oil
MIC of M: B = MIC of in Melaleuca ericifolia oil in combination with benzethonium chloride
The results of above test indicate a combination of benzethonium chloride in the range of 0.0181 ppm to 0.075 ppm, and Melaleuca ericifolia oil in the range of 3125 ppm to 6250 ppm, surprisingly shows synergy in inhibiting the growth of S. aureus.
[00123] Individual MIC data of benzethonium chloride and Melaleuca ericifolia oil samples (or the positive data) is indicated in Table 7.
Table 7. Individual MIC data of benzethonium chloride and Melaleuca ericifolia oil


The resuus 01 aoove test indicate individual IVIIL. data 01 u.z^t^t ppm ior benzethonium chloride and 25000 ppm for Melaleuca ericifolia on the inhibition of S. aureus.
[00124] Results of test for combined concentrations of benzethonium chloride and Melaleuca ericifolia oil samples, that demonstrate indifferent anti-microbial effect on S. aureus is indicated in Table 8.
Table 8.
Results of combination of benzethonium chloride and Melaleuca ericifolia oil with
indifferent effect.
MIC oi'B: JV1= MIC of benzethonium chloride in combination with Melaleuca ericifolia oil
MIC of M: B = MIC of in Melaleuca ericifolia oil in combination with benzethonium chloride
The results of above test indicate a combination of benzethonium chloride in the range of 0.15 ppm to 0.244 ppm, and Melaleuca ericifolia oil in the range of 12500 ppm to 25000 ppm that shows indifferent effect on the inhibition of growth of S. aureus.
Example 4
[00125] Effect of combination of benzethonium chloride and linalool (Ex Basil) on
growth/inhibition of S. aureus. Combination of benzethonium chloride and linalool

(Ex Basil) at various concentrations was tested for its effect on inhibiting growth of S.
aureus.
[00126] The synergistic effect of benzethonium chloride and linalool (Ex Basil)
samples is indicated in Table 9.
Table 9. Results of test for synergistic effect of benzethonium chloride and linalool (Ex Basil)

MIC of B: Li= MIC of benzethonium chloride in combination with linalool (Ex Basil) MIC of Li: B = MIC of linalool (Ex Basil) in combination with benzethonium chloride
The results of above test indicate a combination of benzethonium chloride in the range of 0.018 ppm to 0.037 ppm, and linalool (Ex Basil) in the range of 1562.5 ppm to 2500 ppm that surprisingly shows synergy in inhibiting the growth of S. aureus.
[00127] Individual MIC data of benzethonium chloride and linalool (Ex Basil) samples (or the positive data) is indicated in Table 10.
Table 10. Individual MIC data of benzethonium chloride and linalool (Ex Basil).


The results of above test indicate individual MIC data of 0.244 ppm for benzethonium chloride and 6250 ppm for linalool (Ex Basil) on the inhibition of S. aureus.
[00128] Results of test for combined concentrations of benzethonium chloride and linalool (Ex Basil) samples, that demonstrate indifferent anti-microbial effect on S. aureus is indicated in Table 11.
Table 11. Results of combination of benzethonium chloride and linalool (Ex Basil) with
indifferent effect.
MIC of La: Li= MIC of benzethonium chloride in combination with linalool (Ex Basil) MIC of Li: La = MIC of linalool (Ex Basil) in combination with benzethonium chloride
The results of above test indicate a combination of benzethonium chloride in the range of 0.075 ppm to 0.244 ppm, and linalool (Ex Basil) in the range of 2500 ppm to 6250 ppm that shows indifferent effect on the inhibition of growth of S. aureus.
Example 5
[00129] Method of preparation of a formulation comprising of (a) benzethonium chloride and (b) Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil) is explained in the following steps. Laurie acid, Myristic acid and Palmitic acid were melted at 75-80°C in water bath. A 10% solution of neutralizing agent KOH was prepared and added drop by drop to a mixture of benzethonium chloride

and Trachyspermum copticum oil or Melaleuca ericifolia oil or linalool (Ex Basil). This was mixed at 50-60 rpm. After the neutralization, PEG-150 Distearate was added followed by adding Glycerin at 75°C and then mixed at 50-60 rpm. This was followed by adding fragrance at 40°C and then adding Phenoxyethanol and mixed till
5 all the components are mixed properly at 50-60 rpm. Next Sodium Chloride was added to adjust the viscosity. Finally, water was added to make up the volume to 100%.
[00130] Details of ingredients for example of formulation obtained by the method disclosed herein, that comprises of benzethonium chloride and Trachyspermum
0 copticum oil is indicated in Table 12.

[00131] Details of ingredients for an example of formulation, obtained by the method disclosed herein, that comprises of benzethonium chloride and Melaleuca ericifolia oil are indicated in Table 13.

[00132] Details of ingredients for an example of formulation, obtained by the method disclosed herein, that comprises of benzethonium chloride and linalool (Ex Basil) are indicated in Table 14.

Example 6
[00133] Method of preparation of a topical application formulation comprising benzethonium chloride and Melaleuca ericifolia oil includes: (a) mixing water, disodium EDTA, carbomer, and acrylates/C10-30 alkyl acrylate cross polymer under constant stirring at 300-500RPM, preferably 300RPM for 15-20 minutes at a temperature in the range of 22-28°C to obtain a mixture A; (b) mixing glycerin, and methylparaben to said mixture A with constant stirring at 600RPM for 10-15 minutes at a temperature in the range of 22-28°C to obtain a mixture B; (c) mixing triethanolamine with said mixture B under constant stirring at about 600RPM for 15-20 minutes at a temperature in the range of 22-28°C to obtain a mixture C; (d) mixing benzethonium chloride and Melaleuca ericifolia oil with said mixture C with constant stirring and 500-600 rpm for about 10 minutes; and (e) mixing perfume to mixture C and stirring at 500-600 rpm for about 10 minutes at a temperature in the range of 22-28°C to obtain said formulation.
[00134] Details of ingredients for an example of topical application formulation, that comprises benzethonium chloride and Melaleuca ericifolia oil are indicated in Table 15.

While this example describes topical application formulation using benzethonium chloride with Melaleuca ericifolia oil, it will be understood that a similar formulation can be prepared using benzethonium chloride with Trachyspermum copticum oil or linalool (Ex Basil).
Example 7
[00135] Details of ingredients for an example of hand wash formulation, that includes benzethonium chloride and Trachyspermum copticum oil are indicated in Table 16.

[00136] Details of ingredients for an example of face wash formulation, that comprises of benzethonium chloride and Trachyspermum copticum oil are indicated in Table 17.

Example 9
[00137] Details of ingredients for an example of soap bar formulation, that comprises of benzethonium chloride and Trachyspermum copticum oil are indicated in Table 18.

While the examples 7-9 describe formulations using benzethonium chloride with Trachyspermum copticum oil, it will be understood that a similar formulation can be prepared using benzethonium chloride with Melaleuca ericifolia oil or linalool (Ex Basil).
[00138] Overall, the present disclosure provides a composition for inhibiting growth of pathogenic microorganisms causing skin infections, the said composition comprises of benzethonium chloride and at least one essential oil, wherein essential oil is selected from the group consisting of Trachyspermum copticum oil, Melaleuca ericifolia oil, and linalool (Ex Basil) which at particular synergistic concentration ranges, surprisingly exhibits synergistic effect on inhibition of growth of pathogenic microorganisms causing skin infections. This effect is only apparent within particular concentration ranges, as data presented above clearly shows that not all ranges give a synergistic effect. The combination of the present disclosure is unexpected as not all combinations tested show a surprising non-additive effect, which is a deviation from the expected results.
[00139] Although the present subject matter has been described with reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the subject matter, will become apparent to persons skilled in the art upon reference to the description of the subject matter.

I/We claim:
1. A topical anti-microbial composition comprising synergistic
concentrations of:
i. benzethonium chloride, and
ii. at least one essential oil selected from the group consisting of Trachyspermum copticum oil, Melaleuca ericifolia oil and linalool.
2. The composition as claimed in claim 1, wherein the synergistic
concentration of benzethonium chloride is in the range of 0.018 - 0.075
ppm.
3. The composition as claimed in any of the claims 1-2, wherein the
synergistic concentration of at least one essential oil selected from:
i. Trachyspermum copticum oil in the range of 195.31 - 781.25 ppm,
and ii. Melaleuca ericifolia oil in the range of 3125 - 6250 ppm.
4. The composition as claimed in claim 1, wherein the synergistic concentration of benzethonium chloride is in the range of 0.018 - 0.037 ppm.
5. The composition as claimed in any of the claims 1 or 4, wherein the synergistic concentration of essential oil linalool is in the range of 1562.5 - 2500 ppm.
6. The composition as claimed in any of the claims 1-3, wherein the said composition comprises benzethonium chloride in the range of 0.018 -0.075 ppm, and Trachyspermum copticum oil in the range of 195.31 -781.25 ppm.
7. The composition as claimed in any of the claims 1-3, wherein said composition comprises benzethonium chloride in the range of 0.018 -0.075 ppm, and Melaleuca ericifolia oil in the range of 3125 - 6250 ppm.

8. The composition as claimed in any of the claims 1 or 4, wherein said composition comprises benzethonium chloride in the range of 0.018 -0.037 ppm, and linalool in the range of 1562.5 - 2500 ppm.
9. The composition as claimed in any of the claims 1-3 or 6, wherein the ratio of benzethonium chloride to Trachyspermum copticum oil is in the range of 1:2500 to 1:43500.
10. The composition as claimed in any of the claims 1-3 or 7, wherein the ratio of benzethonium chloride to Melaleuca ericifolia oil is in the range of 1:41000 to 1:350000.
11. The composition as claimed in any of the claims 1 or 4-5 or 8, wherein the ratio of benzethonium chloride to linalool is in the range of 1:40000 to 1: 140000.
12. An anti-microbial formulation comprising a composition as claimed in any of the claims 1-11.
13. The formulation as claimed in claim 12, further comprising one or more cosmetically acceptable excipients selected from the group consisting of fatty acids, alkalis, viscosity modifiers, polymers, humectants, colors, fragrances, surfactants, solubilizers, opacifiers, binders, preservatives, neutralizing agents, chelating agents, emulsifying agents, emollients, and diluents.
14. The formulation as claimed in claim 13, wherein
i. weight concentration of at least one fatty acid is in the range of 10 to
70 %; ii. weight concentration of at least one surfactant is in the range of 0.1
to 20 %; iii. weight concentration of at least one polymer is in the range of 0.005-
2%;

iv. weight concentration of at least one chelating agent is in the range of 0.001-5 %;
v. weight concentration of at least one emollient is in the range of 0.001-10%;
vi. weight concentration of at least one humectant is in the range of
0.01-5 %; vii. weight concentration of at least one emulsifying agent is in the range
of 0.01-5%; viii. weight concentration of at least one fragrance is in the range of 0.01-3 %; and
ix. weight concentration of at least one cosmetically acceptable additive is in the range of 0.001-5 %. 15. The formulation as claimed in any of the claims 12-14, wherein
i. at least one polymer is selected from the group consisting of (a) at least one starch; (b) at least one modified starch; (c) at least one gum; (d) at least one carbomer; (e) at least one silicone based polymer; (f) at least one acrylate; (g) at least one cellulose based polymer; and (h) combinations thereof;
ii. at least one chelating agent is selected from the group consisting of dimercapto-succinic acid (DMSA), dimercapto-propane sulfonate (DMPS), alpha lipoic acid (ALA), calcium disodium versante (CaNa2-EDTA), disodium EDTA, dimercaprol (BAL), etidronic acid, ethyleneglycoltetraacetic acid, ehtylenediaminedisuccinic acid, and combinations thereof;
iii. at least one emollient is selected from the group consisting of lanolin, octyldodecanol, hexyl decanol, oleyl alcohol, decyloleate, isopropyl stearate, isopropyl palmitate, isopropyl myri state, hexyl laureate, dioctyl cyclohexane, PPG-15 stearyl ether, isohexadecane,

stearic acid, cetyl alcohol, mineral oil, at least one ester, at least one
alcohol, at least one essential oil and extracts thereof, at least one
silicone oil, linear polydimethylsiloxanes, cyclic
polydimethylsiloxanes, volatile silicones, and combinations thereof;
iv. at least one humectant selected from the group consisting of at least one silicone, at least one silicone oil, at least one silicone polymer, at least one cyclized derivative of silicone, at least one polyol, at least one acrylate polymer, at least one biopolymer, and combinations thereof;
v. at least one emulsifying agent selected from the group consisting of oxyalkylenated fatty acid esters of polyols, PEG-100 stearate & glyceryl stearate, oxyalkylenated fatty acid esters of sorbitan, oxyalkylenated (oxyethylenated and/or oxypropylenated) fatty alcohol ethers, alkoxylated or non-alkoxylated sugar esters, sorbitan esters, esters of diacids, esters of fatty alcohols, acrylates, PEG based emulsifiers, polyglyceryl based emulsifiers, silicones, dimethicones, mixture of glycerylmonostearate and of polyethylene glycol stearate and combinations thereof;
vi. at least one fragrance selected from the group consisting of blend of essential oils, blend of synthetic aroma compounds, and combination thereof; and
vii. at least one cosmetically acceptable additive is selected from the group consisting of colorants, antioxidants, free radical scavengers, vitamins, anti-aging agents, thickeners, and combinations thereof. 16. An anti-microbial personal cleansing formulation, comprising:
i. at least one C8 to C18 fatty acid in a range of 10 to 25 % by weight;
ii. at least one alkali in a range of 0.001 to 5 % by weight;
iii. at least one solubilizer in a range of 5 to 40 % by weight;

iv. optionally, at least one chelating agent in a range of 0.001 to 5 % by
weight; v. benzethonium chloride in a range of 0.018 - 0.075 ppm; vi. Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or
Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and vii. water to make up 100 % weight.
17. An anti-microbial personal cleansing formulation, comprising:
i. at least one C8 to C18 fatty acid in a range of 10 to 25 % by weight; ii. at least one alkali in a range of 0.001 to 5 % by weight; iii. at least one solubilizer in a range of 5 to 40 % by weight; iv. optionally, at least one chelating agent in a range of 0.001 to 5 % by
weight; v. benzethonium chloride in a range of 0.018 - 0.037 ppm; vi. linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and vii. water to make up 100 % weight.
18. An anti-microbial personal cleansing formulation, comprising:
i. at least one surfactant in a range of 2 to 20 % by weight; ii. at least one polymer in a range of 0.1 to 15 % by weight; iii. at least one solubilizer in a range of 0 to 10 % by weight; iv. optionally, at least one chelating agent in a range of 0.001-5 % by
weight; v. benzethonium chloride in a range of 0.018 - 0.075 ppm; vi. Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or
Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and vii. water to make up 100 % weight.
19. An anti-microbial personal cleansing formulation, comprising:
i. at least one surfactant in a range of 2 to 20 % by weight; ii. at least one polymer in a range of 0.1 to 15 % by weight;

iii. at least one solubilizer in a range of 0 to 10 % by weight;
iv. optionally, at least one chelating agent in a range of 0.001-5 % by
weight; v. benzethonium chloride in a range of 0.018 - 0.037 ppm; vi. linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and vii. water to make up 100 % weight.
20. An anti-microbial soap bar formulation, comprising:
i. at least one sodium salt of fatty acid in a range of 30-95 % by
weight; ii. at least one humectant and emollient in a range of 0.5 to 10 % by
weight; iii. at least one binder in a range of 0.5 to 10 % by weight; iv. optionally at least one opacifier in a range of 0.001 to 3 % by
weight; v. optionally, at least one chelating agent in a range of 0.001-5 % by
weight; vi. benzethonium chloride in a range of 0.018 - 0.075 ppm; vii. Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or
Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and viii. water to make up 100 % weight.
21. An anti-microbial soap bar formulation, comprising:
i. at least one sodium salt of fatty acid in a range of 30-95 % by
weight; ii. at least one humectant and emollient in a range of 0.5 to 10 % by
weight; iii. at least one binder in a range of 0.5 to 10 % by weight; iv. optionally at least one opacifier in a range of 0.001 to 3 % by
weight;

v. optionally, at least one chelating agent in a range of 0.001-5 % by
weight; vi. benzethonium chloride in a range of 0.018 - 0.037 ppm; vii. linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and viii. water to make up 100 % weight.
22. A topical anti-microbial formulation, comprising:
i. at least one chelating agent in a range of 0.001-5 % by weight;
ii. at least one polymer in a range of 0.005-5 % by weight; iii. at least one emulsifying agent in a range of 0.01-5 % by weight; iv. at least one humectant in a range of 0.01-10 % by weight;
v. at least one preservative in a range of 0.001-1 % by weight; vi. at least one neutralizing agent in a range of 0-0.75 % by weight; vii. benzethonium chloride in a range of 0.018 - 0.075 ppm; viii. Trachyspermum copticum oil in a range of 195.31 - 781.25 ppm or Melaleuca ericifolia oil in a range of 3125 - 6250 ppm; and
ix. water to make up 100 % weight.
23. A topical anti-microbial formulation, comprising:
i. at least one chelating agent in a range of 0.001-5 % by weight;
ii. at least one polymer in a range of 0.005-5 % by weight; iii. at least one emulsifying agent in a range of 0.01-5 % by weight; iv. at least one humectant in a range of 0.01-10 % by weight;
v. at least one preservative in a range of 0.001-1 % by weight; vi. at least one neutralizing agent in a range of 0-0.75 % by weight; vii. benzethonium chloride in a range of 0.018 - 0.037 ppm;

viii. linalool (Ex Basil) in a range of 1562.5 - 2500 ppm; and ix. water to make up 100 % weight.