Abstract: The present invention relates to a Bio-microbial consortium Formulation. The bio- microbial formulation comprising. Herein, the Part A comprises of a bacterial composition, a Potash mobilizing bacteria KMB Frateuria aurantia, isolated from farm soil, a Zink Solubilizing Bacteria Pseudomonas aeruginosa, a Zink Solubilizing 5 Bacteria Pseudomonas aeruginosa, a Trichoderma, a Mycorrhiza sp, the Mycorrhiza sp is isolated from roots of wheatgrass, Humic Acid in the range of 5-15 %;w/v, Bio- enzymes in the range of 3-5%;v/v, Rock Phosphate in the range of 500 gm – 1500 gm, Mustard cake in the range of 500 gm – 2000 gm, Amrit mitti in the range of 40-50 gram, and Jaggery 1000 gm -2000 gm. Herein, the Part B comprising an indigenous 10 Cow dung in the range of 2-10kg and indigenous Cow urine.
Claims:1. A Bio-microbial consortium Formulation comprising a full-spectrum of the essential mineral, carbon-organic, and microbiological nutrients for soil and plant health incorporated into a composite blended-media chosen from the group 5 comprising liquid, powder, granular, and slurry. 2. The formulation claimed in claim 1, wherein the formulation comprises of : a Part A, the Part A includes ingredients comprising a) a bacterial composition, the bacterial composition consist of a mixture 10 of organisms selected from but not limited to Bacillus Subtilis, Bacillus megaterium, Azospirillum spp, Azotobacter spp, and Rhizobium spp; b) a Potash mobilizing bacteria KMB Frateuria aurantia, isolated from farm soil; c) a Zink Solublizing Bacteria Pseudomonas aeruginosa; 15 d) a Trichoderma; e) a Mycorrhiza sp, the Mycorrhiza sp is isolated from roots of wheat grass; f) Humic Acid in the range of 5-15 %;w/v g) Bio-enzymes in the range of 3-5%;v/v 20 h) Rock Phosphate in the range of 500 gm – 1500 gm; i) Mustard cake in the range of 500 gm – 2000 gm; j) Amrit mitti in the range of 40-50 gram;and k) Jaggrey 1000 gm -2000 gm 25 a Part B, the Part B includes ingredients comprising a) an indigenous Cow dung in the range of 2-10kg is collected , the indigenous Cow dung is not older than 3-7 days; and b) an indigenous Cow urine is collected, indigenous Cow urine is older than 15-20 day. 30 3. The formulation claimed in claim 1, wherein the formulation consists of all of said ingredients. 4. The formulation claimed in claim 1, wherein the formulation is a liquid.
9 5. The method for the preparation of the microbial consortium formulation used as a microbiological nutrients for soil and plant health, the method consists of: Mixing part A in equal proportion and put for incubation at temperature 25- 30oC; After reaching to optimum microbial cell count and the color changes to off 5 white to pale yellow to feint brown the material of part B is mixed and again put for incubation in natural condition at temperature 25-30oC, resulting in a solution; After the optimum microbial cell count 3X10^8 and more the said solution is filtered with the filter size 2-8 micron and use as dilution combination in respective 10 fields. , Description:BIO MICROBIAL ( CONSORTIUM) LIQUID FERTILIZER FIELD OF THE INVENTION The present invention relates to a microbial consortium formulation used as a 5 microbiological nutrient for soil and plant health. More particularly, the present invention relates to a bio-microbial formulation intended to restore soil structure, increase plant yields, neutralize toxic contaminants, protect the ecological food web and replenish vital nutrients. 10 BACKGROUND OF THE INVENTION One of the most critical problems associated with intensive agricultural activities has been the degradation of the natural environment due to the overuse of chemical fertilizers. Research has shown that the potential problems associated with the overuse of chemical fertilizers may outweigh the benefits of increases in crop production. 15 However, despite this, it has been predicted that “the use of chemical fertilizers must be expanded two- to threefold to maintain soil fertility and productivity in the developing countries over the next 25 years if the world is to feed itself.”. There are a number of reasons why increased use of chemical fertilizers has been 20 predicted, including increased human population and the replacement of traditional foods with cereals like wheat. However, a more worrying reason is that yields from traditional agricultural lands are falling. Although the reason for this is not known, even more, chemical fertilizer has usually been used in an attempt to reverse the trend. However, one factor that has been noted is that the amount of topsoil in these areas 25 has steadily been declining, and that normal microbial activity is reduced The green insurgency acquired astonishing results in food grain creation however with lacking concern for farming maintainability. The accessibility and reasonableness of petroleum product-based synthetic manures at the ranch level in 30 India have been guaranteed uniquely through imports and sponsorships which are generally reliant upon the GDP of the country. Reliance on synthetic substances for future farming requirements would bring about additional misfortune in soil wellbeing, conceivable outcomes of water tainting, and determined weight on the monetary
2 framework. Unpredictable engineered compost use has contaminated the dirt, water bowls, obliterated miniature life forms, and eco-accommodating creepy crawlies, made the yield more helpless to illnesses, and drained soil richness at the essential levels starting today, which is the fundamental concern of the development. In this basic setting, Microorganisms have arisen as the possible option for the efficiency, 5 dependability, and manageability of the worldwide natural way of life. Notwithstanding the aforementioned approaches, there remains a need is a need for an alternative to chemical fertilizers which increases crop production, while minimizing the degradation of the environment. In particular, there is a need to have an alternative 10 that reduces the incidence of disease in plants, improves root health and improves the levels of organic matter in the soil. OBJECTIVE OF THE PRESENT INVENTION 15 The main objective of the present invention is to provide a microbial consortium formulation used as a microbiological nutrient for soil and plant health. Another objective of the present invention is to provide a formulation used to improve plant growth, natural immunity, and resilience to change, as well as improve the 20 condition of the soil and protect the soil food web and larger ecosystem. Yet another objective of the present invention is to provide a chemical-free formulation. 25 Yet another objective of the present invention is to provide a formulation that is sustainable for the farmer, society, climate, economics and biodiversity. Yet another objective of the present invention is to provide a mineral-rich formulation used to manage/ improve the water level of the soil and provide better 30 growth/yield/health to the plant/crop in the soil/farmland.
3 Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided hereinbelow, in which various embodiments of the disclosed invention are illustrated by way of example SUMMARY OF THE INVENTION 5 The present invention relates to a Bio-microbial consortium Formulation. The bio- microbial formulation comprising a full spectrum of the essential mineral, carbon- organic, and microbiological nutrients for soil and plant health incorporated into a composite blended media chosen from the group comprising liquid, powder, granular, and slurry. The formulation comprises a Part A and a Part B. Herein, the Part A 10 comprises of a bacterial composition, a Potash mobilizing bacteria KMB Frateuria aurantia, isolated from farm soil, a Zink Solubilizing Bacteria Pseudomonas aeruginosa, a Zink Solubilizing Bacteria Pseudomonas aeruginosa, a Trichoderma, a Mycorrhiza sp, the Mycorrhiza sp is isolated from roots of wheatgrass, Humic Acid in the range of 5-15 %;w/v, Bio-enzymes in the range of 3-5%;v/v, Rock Phosphate in 15 the range of 500 gm – 1500 gm, Mustard cake in the range of 500 gm – 2000 gm, Amrit mitti in the range of 40-50 gram, and Jaggery 1000 gm -2000 gm. The bacterial composition consists of a mixture of organisms selected from but not limited to Bacillus Subtilis, Bacillus megaterium, Azospirillum spp, Azotobacter spp, and Rhizobium spp.Herein, the Part B comprising an indigenous Cow dung in the range 20 of 2-10kg is collected, the indigenous Cow dung is not older than 3-7 days, and indigenous Cow urine is collected, indigenous Cow urine is older than 15-20 days. The formulation consists of all of the said ingredients comprising both the parts Part A and Part B. Herein the formulation is a liquid form. The present invention relates to a method for the preparation of the microbial consortium formulation used as a 25 microbiological nutrient for soil and plant health. The method consists of mixing part A in equal proportion and put for incubation at a temperature 25-30oC. After reaching optimum microbial cell count and the color changes to off-white to pale yellow to faint brown the material of part B is mixed and again put for incubation in natural condition at temperature 25-30oC, resulting in a solution. After the optimum microbial 30 cell count, 3X10^8 and more the said solution is filtered with the filter size 2-8 micron and used as a dilution combination in respective fields.
4 The main advantage of the present invention is to provide a microbial consortium formulation used as a microbiological nutrient for soil and plant health. Another advantage of the present invention is to provide a formulation used to improve plant growth, natural immunity and resilience to change, as well as improve 5 the condition of the soil and protect the soil food web and larger ecosystem. Yet another advantage of the present invention is to provide a chemical free formulation. 10 Yet another advantage of the present invention is to provide a formulation that is sustainable for the farmer, society, climate, economics and biodiversity. Yet another advantage of the present invention is to provide a mineral rich formulation use to manage or improve the water level of the soil and provide better 15 growth/yield/health to the plant/crop in the soil/farm land. Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided hereinbelow, in which various embodiments of the disclosed invention are illustrated by way of example 20 DETAILED DESCRIPTION OF THE INVENTION Definition 25 The term “a” or “an”, as used herein, is defined as one. The term “plurality”, as used herein, is defined as two as or more than one. The term “another”, as used herein, is defined as at least a second or more. The terms “including” and/or “having”, as used herein, are defined as comprising (i.e., open language). 30 The term “comprising” is not intended to limit the present invention with such terminology rather is used in a wider sense. Any invention using the term comprising could be separated into one or more claims using “consisting” or “consisting of”. The
5 term “comprising” may be used interchangeably with the terms “having” or “containing”. Reference in this document to “one embodiment”, “certain embodiments”, “an embodiment”, “another embodiment”, and “yet another embodiment” or similar terms, 5 throughout the document means that a specific feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of such phrases in various places, this specification throughout are not necessarily all referring to the same embodiment. Furthermore, the specific features, structures, or characteristics are combined in any 10 suitable manner in one or more embodiments without limitation. The term “or” as used herein is to be interpreted as inclusive or meaning any one or more combinations. Therefore, “A, B or C” means any of the following: “A; B; C; A and B; A and C; B and C; A, B and C”. An exception to this definition will occur only 15 when a combination of elements, functions, steps, or acts are in mutually exclusive, inherently. As used herein, the term "one or more" generally refers to, but is not limited to, singular as well as the plural form of the term. 20 The drawings featured in the figures are to illustrate certain convenient embodiments of the present invention and are not to be considered as a limitation to that. Term "means" preceding a present participle of operation indicates the desired function for which there is one or more embodiments, i.e., one or more methods, for achieving the 25 desired function and that one skilled in the art could select from these or their equivalent in view of the disclosure herein and use of the term "means" is not intended to be limiting. The present invention relates to a Bio-microbial consortium Formulation. The bio- 30 microbial formulation comprising a full spectrum of the essential mineral, carbon- organic, and microbiological nutrients for soil and plant health incorporated into a composite blended media chosen from the group comprising liquid, powder, granular, and slurry.
6 In an embodiment of the present invention, the formulation comprises a Part A and a Part B. Herein, the Part A comprises of a bacterial composition, a Potash mobilizing bacteria KMB Frateuria aurantia, isolated from farm soil, a Zink Solubilizing Bacteria Pseudomonas aeruginosa, a Zink Solubilizing Bacteria Pseudomonas 5 aeruginosa, a Trichoderma, a Mycorrhiza sp, the Mycorrhiza sp is isolated from roots of wheatgrass, Humic Acid in the range of 5-15 %;w/v, Bio-enzymes in the range of 3-5%;v/v, Rock Phosphate in the range of 500 gm – 1500 gm, Mustard cake in the range of 500 gm – 2000 gm, Amrit mitti in the range of 40-50 gram, and Jaggery 1000 gm -2000 gm. The bacterial composition consist of a mixture of 10 organisms selected from but not limited to Bacillus Subtilis, Bacillus megaterium, Azospirillum spp, Azotobacter spp, and Rhizobium spp. Herein, the Part B comprising an indigenous Cow dung in the range of 2-10kg is collected, the indigenous Cow dung is not older than 3-7 days, and indigenous Cow 15 urine is collected, indigenous Cow urine is older than 15-20 days. In the preferred embodiment of the present invention, the formulation consists of all of the said ingredients comprising both the parts Part A and Part B. Herein the formulation is a liquid form. 20 The present invention relates to a method for the preparation of the microbial consortium formulation used as a microbiological nutrient for soil and plant health. The method consists of mixing part A in equal proportion and put for incubation at a temperature 25-30oC. After reaching optimum microbial cell count and the color 25 changes to off-white to pale yellow to faint brown the material of part B is mixed and again put for incubation in natural condition at temperature 25-30oC, resulting in a solution. After the optimum microbial cell count, 3X10^8 and more the said solution is filtered with the filter size 2-8 micron and used as a dilution combination in respective fields. 30 Those of skill in the art will appreciate that the application of the microbial consortium formulation used as a microbiological nutrient for soil may promote the
7 growth and viability of the bacterial population present in the composition and the existing soil microflora. Such persons will further appreciate that the microbial consortium formulation may provide protection of seeds, promote healthy development and result in higher rates of 5 seed germination in primary industry applications compared to untreated soil. In one preferred embodiment of the invention, seeds may be coated with the microbial consortium formulation of the present invention before planting or before packaging for sale. 10 Further objectives, advantages, and features of the present invention will become apparent from the detailed description provided herein, in which various embodiments of the disclosed present invention are illustrated by way of example and appropriate reference to accompanying drawings. Those skilled in the art to which the present invention pertains may make modifications resulting in other embodiments employing 15 principles of the present invention without departing from its spirit or characteristics, particularly upon considering the foregoing teachings. Accordingly, the described embodiments are to be considered in all respects only as illustrative, and not restrictive, and the scope of the present invention is, therefore, indicated by the appended claims rather than by the foregoing description or drawings. Consequently, 20 while the present invention has been described with reference to particular embodiments, modifications of structure, sequence, materials and the like apparent to those skilled in the art still fall within the scope of the invention as claimed by the applicant.
| # | Name | Date |
|---|---|---|
| 1 | 202241009374-STATEMENT OF UNDERTAKING (FORM 3) [22-02-2022(online)].pdf | 2022-02-22 |
| 2 | 202241009374-POWER OF AUTHORITY [22-02-2022(online)].pdf | 2022-02-22 |
| 3 | 202241009374-FORM FOR STARTUP [22-02-2022(online)].pdf | 2022-02-22 |
| 4 | 202241009374-FORM FOR SMALL ENTITY(FORM-28) [22-02-2022(online)].pdf | 2022-02-22 |
| 5 | 202241009374-FORM 1 [22-02-2022(online)].pdf | 2022-02-22 |
| 6 | 202241009374-EVIDENCE FOR REGISTRATION UNDER SSI(FORM-28) [22-02-2022(online)].pdf | 2022-02-22 |
| 7 | 202241009374-EVIDENCE FOR REGISTRATION UNDER SSI [22-02-2022(online)].pdf | 2022-02-22 |
| 8 | 202241009374-DECLARATION OF INVENTORSHIP (FORM 5) [22-02-2022(online)].pdf | 2022-02-22 |
| 9 | 202241009374-COMPLETE SPECIFICATION [22-02-2022(online)].pdf | 2022-02-22 |
| 10 | 202241009374-Correspondence_Form-26_18-04-2022.pdf | 2022-04-18 |
| 11 | 202241009374-FORM-9 [19-05-2022(online)].pdf | 2022-05-19 |
| 12 | 202241009374-STARTUP [20-06-2022(online)].pdf | 2022-06-20 |
| 13 | 202241009374-FORM28 [20-06-2022(online)].pdf | 2022-06-20 |
| 14 | 202241009374-FORM 18A [20-06-2022(online)].pdf | 2022-06-20 |
| 15 | 202241009374-Correspondence_SIPP Scheme_15-07-2022.pdf | 2022-07-15 |
| 16 | 202241009374-FER.pdf | 2022-07-20 |
| 17 | 202241009374-Correspondence_SIPP Scheme_11-08-2022.pdf | 2022-08-11 |
| 18 | 202241009374-FORM 4(ii) [19-01-2023(online)].pdf | 2023-01-19 |
| 19 | 202241009374-OTHERS [20-02-2023(online)].pdf | 2023-02-20 |
| 20 | 202241009374-FORM 3 [20-02-2023(online)].pdf | 2023-02-20 |
| 21 | 202241009374-FER_SER_REPLY [20-02-2023(online)].pdf | 2023-02-20 |
| 22 | 202241009374-CORRESPONDENCE [20-02-2023(online)].pdf | 2023-02-20 |
| 23 | 202241009374-COMPLETE SPECIFICATION [20-02-2023(online)].pdf | 2023-02-20 |
| 24 | 202241009374-CLAIMS [20-02-2023(online)].pdf | 2023-02-20 |
| 25 | 202241009374-ABSTRACT [20-02-2023(online)].pdf | 2023-02-20 |
| 26 | 202241009374-US(14)-HearingNotice-(HearingDate-04-05-2023).pdf | 2023-04-06 |
| 27 | 202241009374-REQUEST FOR ADJOURNMENT OF HEARING UNDER RULE 129A [03-05-2023(online)].pdf | 2023-05-03 |
| 28 | 202241009374-US(14)-ExtendedHearingNotice-(HearingDate-20-06-2023).pdf | 2023-05-18 |
| 29 | 202241009374-Correspondence to notify the Controller [13-06-2023(online)].pdf | 2023-06-13 |
| 30 | 202241009374-Written submissions and relevant documents [05-07-2023(online)].pdf | 2023-07-05 |
| 31 | 202241009374-RELEVANT DOCUMENTS [05-07-2023(online)].pdf | 2023-07-05 |
| 32 | 202241009374-POA [05-07-2023(online)].pdf | 2023-07-05 |
| 33 | 202241009374-PETITION UNDER RULE 137 [05-07-2023(online)].pdf | 2023-07-05 |
| 34 | 202241009374-MARKED COPIES OF AMENDEMENTS [05-07-2023(online)].pdf | 2023-07-05 |
| 35 | 202241009374-FORM 13 [05-07-2023(online)].pdf | 2023-07-05 |
| 36 | 202241009374-AMMENDED DOCUMENTS [05-07-2023(online)].pdf | 2023-07-05 |
| 37 | 202241009374-NBA Approval Submission [23-12-2024(online)].pdf | 2024-12-23 |
| 38 | 202241009374-NBA Approval Submission [05-08-2025(online)].pdf | 2025-08-05 |
| 39 | 202241009374-PatentCertificate08-08-2025.pdf | 2025-08-08 |
| 40 | 202241009374-IntimationOfGrant08-08-2025.pdf | 2025-08-08 |
| 1 | SearchE_20-07-2022.pdf |